Rw5G047200

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000273
Physical Location & Seq
Reverse (-)
81696886 .. 81711997
15112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047200.1

Sequence Viewer

Length: 2817 bp
ATGGGAAATGAAGGGTATTTACCATTGTTTGAGACCAAGAGAGCAAAGGGAAGAGTCATATATAGGGTGTTTGCTGCTTCTTTGTTTGCAGGTATATGCTTGGTTTGGGTTTATAGGGCGAGTCACATACCAAAACCAGGAGAAGATGGAAGGTTTGGGTGGATGGGATTGTTGGGTGCTGAGTTATGGTTCGGGCTTTATTGGATCCTCACCCAATCCTGCCGATGGAACCTTACCTTTAGGCAAACTTTCAAGGATAGACTATCTCAGAGATTTGAGAATGAGTTGCCGGGAGTGGACATTTTTGTGACCACGGCAGACCCTATCATAGAGCCACCAATGATGGTCATGAACACCGTTCTGTCAGTCATGGCTTATGATTACCCAACCGAGAAGATCAGTGTGTATCTATCCGATGATGGTGGTTCCGAGCTTACATACTACGCACTCTTGGAGGCTGCTGAGTTTGCTAAGCAGTGGATACCATACTGCAAGAAATACAGTGTGGAGCCAAGGTCCCCAGTTGCTTACTTTGTATCAATAGCTACTGATGCAGTTGGTGTTGATGATAAAAAGGCTAATGACTTGGCTGTCATTAAGAAATCATACAAGGACATGGAGACTAAAATCGAAAATGCAGTCAAATTAGGCCACATTCCAGAGAAAGTACGATCGAAACAGAAAGGCTTTTCTCAATGGGACTCACATTTGTCTAAACGTGATCATGACACCTATCTTCAAGTAGTAATTGATGGAAGAGACCCAAAGGCAATCGACGTTGAAGGACTTGTACTGCCAACTTTAGTGTATTTAGCTCGGGAGAAAAGACCACAGTATCACCATAACTTCAAAGCTGGCTCTATGAATGCATTGATTAGGGTTTCTTCAAATATTAGCAACGGGAAGGTCATTCTAAATGTAGACTGTGACATGTATTCAAACAATTCCATGGCCATAAGGGATGCACTTTGCTGCTTAATGGATGAAGAAAAAGGCCATGAAGTTGCATTCGTACAATTCCCACAAAATTTTGAAAACCTCACCAAGAATGAATTATATATTCCATTCATGCGAACAATCAGCGAGGTGGAATTGCATGCTTTGGATGGTTTTGATGGCCCTTTTTATGTTGGAAGTGGATGCTTCCACAGAAGGGATACTCTTTGTGGAAGGAAGTTCACCAAGGGATGTAAGAGTGACATGAAATGGGAGATCAGTAGAAAAAGAGAAGAAACTACAATTCATGAATTGGAAGAAGATTCAAAAGTTCTTGCAAACTGTGCATACGAGCAAAACACTGAGTGGGGAAAAGAGATGGGTTTGAAATATGGTTGTGCAATTGAAGACGTGTTCACGGGGTTATCAATCCAATGCCGCGGTTGGAGATCGGTGTACTTGAACCCAACAAGGAAAGCTTTCTTAGGAGTAGCTCCAACGACATTGCTTCAGATGCTGGTGCAGCAAAAGAGATGGTCGGAAGGTAGTTTCCAGATTTTGCTTTCACGGCACAATCCTGCATTCTCTAATCAGATTAGCTTGGGCCAAAAACTTGGATACGCATATGTTGGGGCTTGGTCTATGAATTTCCTACCAACCCTATTTTACACAGTGATCCCTTCCCTTTACCTCCTTAAGGGCATTTCCTTATTTCCTCGGGTTTCAAGTCCATGGATCATACCATTTGCATATGTGATAATTGCCAAGTACACTGGGAGCTTTTTGGAGTTTCTATGGGCAGGTGGCTCAGCCTTAGGTTGGTGGAACGACCAACGAATGTGGCTATACAAAAGATCAAGCTCCTACATATTTTCCTTCATCGACACCATTTTGTACCTTTTGGGGTATTCTGATTCGGCTTTTGTAATAACAGCCAAGACGTCGGATGAAGATGAGTCGCAACGATATGAGAAAGAGATTATGGAATTCGGAGCCTCTTCTCCACTGTTTTCCGTATTGGCATCACTTGCAGTACTAAATTTGTATTGCTTTGCTGGTTTTGTGAAGGAAGCAATCAGTCTGGGATCAAAGGGCGGCATTGCAGAAGTTTACGATACAATGGCATTGCAAATTCTACTATGTGGGATTTTGATTCTCATCAACCTGCCTTTGTACCAAGCCCTTTATCTAAGGAAGGACAAGGGGAAGTTGCCAAGCTCCATAGCTTTGAAGTCGATGGCATTTGGTATGCAAATTTCAAATTGTGCTGCTGTTACATTGTTGGCTTTTTTATTGAGCCTACAAGGAGCTTTTGGGATCAGATTTGTGATCGACAGAGATGAATGCTTCTCTCATAATGTTTTGTATGAAGGAGATACTGTCCACCTCTCTTTTGTTGTAATTAAGGTTGATGCATCTTGGCATTATACTCAAGACGGTGTTGATCTCCTGGTGAAGGGGCCTTCTGGTGAGCAGATACATGAGTTTCGTGATAAGACAAGCGAGAAATTCGAGTTTGTCACTCATCACAAAGGAATCCATCAGTTTTGTTTCACCAACAAGTCACCTTATCATGAAACCATTGACTTTGACTTACATGTCAGCCATTTTACATACTATGATCAGCATGCAAGAGATGAGCATTTTAACCCTTTGTTAGATCAGATAACAAAGTTAGAGGAAGCTCTTTACAACATTCAGTTTGAGCAGCATTGGCTTGAGGCTCAGACTGAACGCCAGGCAATAGTGAACGACGCAATGAGCCGGAGGGCGATTCACAAGGCCTTTTTTGAATCAGCAGGACTTGTTGGTGCCAGTGTTCTCCAAGTTTACCTTCTTCGCCGCCTCTTTGAACGGAAGCTTGGGACGTCCAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

938

Amino Acids

106.94

Weight (kDa)

6.51

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulose_synt PF03552 99 - 396 1.6e-90 Cellulose synthase
Cellulose_synt PF03552 412 - 671 4.8e-44 Cellulose synthase
EMP24_GP25L PF01105 759 - 930 1.6e-30 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55850
fragaria_vesca FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42770 FvH4_3g42800 FvH4_3g42810 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42900
malus_domestica MD03G1028800.v1.1 MD03G1028900.v1.1 MD03G1029000.v1.1 MD03G1029100.v1.1
prunus_persica Prupe.6G024800_v2.0.a1 Prupe.6G024900_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025100_v2.0.a1 Prupe.6G025100_v2.0.a1
pyrus_communis pycom01g03430 pycom03g02330 pycom03g02340 pycom03g02360 pycom03g02380 pycom03g02400 pycom03g02410 pycom05g13920
rosa_chinensis RchiOBHm_Chr5g0076821 RchiOBHm_Chr5g0076831 RchiOBHm_Chr5g0076851 RchiOBHm_Chr5g0076871 RchiOBHm_Chr5g0076891 RchiOBHm_Chr5g0076901 RchiOBHm_Chr5g0076911 RchiOBHm_Chr5g0076921
rosa_laevigata RLG00000036625 RLG00000036626 RLG00000036627 RLG00000036630 RLG00000036632 RLG00000036634 RLG00000036635
rosa_multiflora Rmu_co8084432.1_g000001 Rmu_co8208886.1_g000001 Rmu_co8325177.1_g000001 Rmu_sc0003799.1_g000001 Rmu_sc0003799.1_g000002 Rmu_sc0003799.1_g000006 Rmu_sc0003799.1_g000009 Rmu_sc0004655.1_g000002 Rmu_sc0004655.1_g000014 Rmu_sc0004655.1_g000015 Rmu_sc0007021.1_g000003 Rmu_sc0007021.1_g000013 Rmu_sc0007021.1_g000014 Rmu_sc0008244.1_g000002 Rmu_sc0013263.1_g000004 Rmu_sc0014402.1_g000001 Rmu_ssc0000184.1_g000003 Rmu_ssc0000184.1_g000004 Rmu_ssc0000184.1_g000005 Rmu_ssc0000184.1_g000006 Rmu_ssc0000184.1_g000014 Rmu_ssc0000184.1_g000021
rosa_roxburghii Rroxscaffold_1G00005210 Rroxscaffold_1G00005220 Rroxscaffold_1G00005250 Rroxscaffold_1G00005280 Rroxscaffold_1G00005300 Rroxscaffold_1G00005310
rosa_rugosa Rorug05G0449200 Rorug05G0449300 Rorug05G0449300 Rorug05G0449300 Rorug05G0449500 Rorug05G0449600
rosa_samantha Rh5AG503600 Rh5AG503700 Rh5AG503900 Rh5AG504000 Rh5AG504100 Rh5BG525000 Rh5BG525200 Rh5BG525300 Rh5BG525500 Rh5BG525700 Rh5BG525900 Rh5BG526000 Rh5CG548600 Rh5CG548800 Rh5CG548900 Rh5CG549100 Rh5CG549300 Rh5CG549500 Rh5DG537300 Rh5DG537400 Rh5DG537500 Rh5DG537700 Rh5DG537900 Rh5DG538000 Rh5DG544100
rosa_wichuraiana Rw0G009920 Rw5G046720 Rw5G046730 Rw5G047200 Rw5G047210 Rw5G047240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1727
AasI GACNNNNNNGTC 2 cut(s) 590, 2534
AatII GACGTC 2 cut(s) 1880, 2807
Acc36I ACCTGC 3 cut(s) 80, 1727, 2109
AccB1I GGYRCC 1 cut(s) 2747
AccI GTMKAC 1 cut(s) 921
AccII CGCG 1 cut(s) 1377
AciI CCGC 4 cut(s) 1375, 1377, 2031, 2779
AclWI GGATC 6 cut(s) 199, 212, 1606, 1679, 2029, 2261
AcoI YGGCCR 1 cut(s) 951
AcsI RAATTY 7 cut(s) 1027, 1582, 1922, 1975, 2067, 2190, 2444
AcuI CTGAAG 1 cut(s) 1430
AcyI GRCGYC 2 cut(s) 1877, 2804
AdeI CACNNNGTG 1 cut(s) 1302
AfaI GTAC 8 cut(s) 669, 792, 1014, 1394, 1706, 1832, 1971, 2111
AfiI CCNNNNNNNGG 6 cut(s) 137, 225, 1153, 1633, 1755, 2392
AflII CTTAAG 1 cut(s) 1631
AflIII ACRYGT 3 cut(s) 930, 1347, 2533
AjiI CACGTC 1 cut(s) 1348
AjnI CCWGG 3 cut(s) 136, 2385, 2673
AjuI GAANNNNNNNTTGG 4 cut(s) 2754, 2781, 2786, 2813
Alw26I GTCTC 3 cut(s) 26, 614, 753
AlwI GGATC 6 cut(s) 199, 212, 1606, 1679, 2029, 2261
AlwNI CAGNNNCTG 2 cut(s) 1453, 2814
Ama87I CYCGRG 2 cut(s) 816, 1653
AoxI GGCC 7 cut(s) 649, 951, 994, 1117, 1540, 2396, 2718
ApeKI GCWGC 6 cut(s) 74, 458, 972, 1459, 2204, 2644
ApoI RAATTY 7 cut(s) 1027, 1582, 1922, 1975, 2067, 2190, 2444
ArsI GACNNNNNNTTYG 2 cut(s) 624, 656
Asp700I GAANNNNTTC 1 cut(s) 1415
AspS9I GGNCC 4 cut(s) 516, 1118, 1540, 2396
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 8 cut(s) 202, 830, 1033, 1171, 2401, 2417, 2482, 2493
AvaI CYCGRG 2 cut(s) 816, 1653
AvaII GGWCC 1 cut(s) 516
AxyI CCTNAGG 1 cut(s) 1750
BaeI ACNNNNGTAYC 4 cut(s) 818, 851, 2405, 2438
BalI TGGCCA 1 cut(s) 953
BamHI GGATCC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 2747
BarI GAAGNNNNNNTAC 3 cut(s) 35, 774, 806
BbsI GAAGAC 1 cut(s) 1350
BbvI GCAGC 6 cut(s) 61, 445, 959, 1471, 2191, 2656
BceAI ACGGC 2 cut(s) 330, 1520
BciT130I CCWGG 3 cut(s) 138, 2387, 2675
BciVI GTATCC 3 cut(s) 474, 1150, 1547
BclI TGATCA 2 cut(s) 721, 2557
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 3 cut(s) 26, 614, 753
BfrI CTTAAG 1 cut(s) 1631
BfuAI ACCTGC 3 cut(s) 80, 1727, 2109
BfuI GTATCC 3 cut(s) 474, 1150, 1547
BisI GCNGC 9 cut(s) 75, 459, 973, 1375, 1460, 2032, 2205, 2645, 2779
BlpI GCTNAGC 2 cut(s) 471, 1744
BlsI GCNGC 9 cut(s) 76, 460, 974, 1376, 1461, 2033, 2206, 2646, 2780
BmcAI AGTACT 1 cut(s) 1971
Bme1390I CCNGG 4 cut(s) 138, 291, 2387, 2675
Bme18I GGWCC 1 cut(s) 516
BmeT110I CYCGRG 2 cut(s) 816, 1653
BmgBI CACGTC 1 cut(s) 1348
BmgT120I GGNCC 4 cut(s) 516, 1118, 1540, 2396
BmiI GGNNCC 8 cut(s) 206, 230, 427, 510, 518, 1930, 2397, 2749
BmrFI CCNGG 4 cut(s) 138, 291, 2387, 2675
BmrI ACTGGG 2 cut(s) 515, 1719
BmsI GCATC 7 cut(s) 541, 952, 1130, 1440, 1967, 2338, 2360
BmuI ACTGGG 2 cut(s) 515, 1719
BpiI GAAGAC 1 cut(s) 1350
Bpu1102I GCTNAGC 2 cut(s) 471, 1744
BpuEI CTTGAG 2 cut(s) 2352, 2675
BpuMI CCSGG 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 2093
BsaHI GRCGYC 2 cut(s) 1877, 2804
BsaI GGTCTC 2 cut(s) 26, 753
BsaJI CCNNGG 7 cut(s) 312, 512, 948, 1182, 1375, 1652, 1667
BsaXI ACNNNNNCTCC 4 cut(s) 500, 530, 2301, 2331
Bsc4I CCNNNNNNNGG 6 cut(s) 137, 225, 1153, 1633, 1755, 2392
Bse1I ACTGG 3 cut(s) 521, 1714, 2751
Bse21I CCTNAGG 1 cut(s) 1750
Bse3DI GCAATG 4 cut(s) 1439, 2034, 2060, 2700
Bse8I GATNNNNATC 1 cut(s) 2093
BseBI CCWGG 3 cut(s) 138, 2387, 2675
BseDI CCNNGG 7 cut(s) 312, 512, 948, 1182, 1375, 1652, 1667
BseGI GGATG 7 cut(s) 168, 967, 988, 1111, 1145, 1193, 1888
BseJI GATNNNNATC 1 cut(s) 2093
BseLI CCNNNNNNNGG 6 cut(s) 137, 225, 1153, 1633, 1755, 2392
BseMI GCAATG 4 cut(s) 1439, 2034, 2060, 2700
BseMII CTCAG 6 cut(s) 171, 281, 453, 1290, 1758, 2675
BseNI ACTGG 3 cut(s) 521, 1714, 2751
BseXI GCAGC 6 cut(s) 61, 445, 959, 1471, 2191, 2656
BsgI GTGCAG 1 cut(s) 1478
Bsh1236I CGCG 1 cut(s) 1377
Bsh1285I CGRYCG 1 cut(s) 674
BshFI GGCC 7 cut(s) 651, 953, 996, 1119, 1542, 2398, 2720
BshNI GGYRCC 1 cut(s) 2747
BsiEI CGRYCG 1 cut(s) 674
BsiHKCI CYCGRG 2 cut(s) 816, 1653
BsiSI CCGG 2 cut(s) 290, 2701
BslFI GGGAC 2 cut(s) 502, 713
BslI CCNNNNNNNGG 6 cut(s) 137, 225, 1153, 1633, 1755, 2392
BsmAI GTCTC 3 cut(s) 26, 614, 753
BsmFI GGGAC 2 cut(s) 502, 713
BsmI GAATGC 4 cut(s) 871, 1007, 1517, 2285
BsnI GGCC 7 cut(s) 651, 953, 996, 1119, 1542, 2398, 2720
Bso31I GGTCTC 2 cut(s) 26, 753
BsoBI CYCGRG 2 cut(s) 816, 1653
Bsp1720I GCTNAGC 2 cut(s) 471, 1744
Bsp19I CCATGG 2 cut(s) 948, 1667
BspACI CCGC 4 cut(s) 1375, 1377, 2031, 2779
BspANI GGCC 7 cut(s) 651, 953, 996, 1119, 1542, 2398, 2720
BspCNI CTCAG 6 cut(s) 172, 280, 454, 1291, 1757, 2674
BspFNI CGCG 1 cut(s) 1377
BspHI TCATGA 4 cut(s) 348, 724, 1243, 2509
BspLI GGNNCC 8 cut(s) 206, 230, 427, 510, 518, 1930, 2397, 2749
BspMI ACCTGC 3 cut(s) 80, 1727, 2109
BspPI GGATC 6 cut(s) 199, 212, 1606, 1679, 2029, 2261
BspT107I GGYRCC 1 cut(s) 2747
BspTI CTTAAG 1 cut(s) 1631
BspTNI GGTCTC 2 cut(s) 26, 753
BsrDI GCAATG 4 cut(s) 1439, 2034, 2060, 2700
BsrI ACTGG 3 cut(s) 521, 1714, 2751
BssECI CCNNGG 7 cut(s) 312, 512, 948, 1182, 1375, 1652, 1667
BssNI GRCGYC 2 cut(s) 1877, 2804
BssT1I CCWWGG 4 cut(s) 512, 948, 1182, 1667
Bst2UI CCWGG 3 cut(s) 138, 2387, 2675
Bst4CI ACNGT 9 cut(s) 358, 503, 834, 926, 1280, 1609, 1944, 2317, 2375
Bst6I CTCTTC 3 cut(s) 46, 751, 1939
BstACI GRCGYC 2 cut(s) 1877, 2804
BstAFI CTTAAG 1 cut(s) 1631
BstAPI GCANNNNNTGC 2 cut(s) 1280, 1964
BstC8I GCNNGC 3 cut(s) 856, 1098, 2565
BstDSI CCRYGG 4 cut(s) 312, 948, 1375, 1667
BstENI CCTNNNNNAGG 2 cut(s) 1631, 2390
BstF5I GGATG 7 cut(s) 168, 967, 988, 1111, 1145, 1193, 1888
BstFNI CGCG 1 cut(s) 1377
BstMAI GTCTC 3 cut(s) 26, 614, 753
BstMCI CGRYCG 1 cut(s) 674
BstMWI GCNNNNNNNGC 8 cut(s) 467, 551, 1280, 1450, 1459, 1504, 1964, 2650
BstNI CCWGG 3 cut(s) 138, 2387, 2675
BstNSI RCATGY 4 cut(s) 934, 1100, 2537, 2567
BstSCI CCNGG 4 cut(s) 136, 289, 2385, 2673
BstUI CGCG 1 cut(s) 1377
BstV1I GCAGC 6 cut(s) 61, 445, 959, 1471, 2191, 2656
BstV2I GAAGAC 1 cut(s) 1350
BstX2I RGATCY 1 cut(s) 204
BstXI CCANNNNNNTGG 1 cut(s) 1550
BstYI RGATCY 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 1750
BsuI GTATCC 3 cut(s) 474, 1150, 1547
BsuRI GGCC 7 cut(s) 651, 953, 996, 1119, 1542, 2398, 2720
BtgI CCRYGG 4 cut(s) 312, 948, 1375, 1667
BtrI CACGTC 1 cut(s) 1348
BtsCI GGATG 7 cut(s) 168, 967, 988, 1111, 1145, 1193, 1888
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 8 cut(s) 406, 482, 508, 1296, 1614, 1707, 1940, 2758
BveI ACCTGC 3 cut(s) 80, 1727, 2109
Cac8I GCNNGC 3 cut(s) 856, 1098, 2565
CaiI CAGNNNCTG 2 cut(s) 1453, 2814
CciI TCATGA 4 cut(s) 348, 724, 1243, 2509
Cfr13I GGNCC 4 cut(s) 516, 1118, 1540, 2396
Cfr42I CCGCGG 1 cut(s) 1378
CseI GACGC 1 cut(s) 2699
Csp6I GTAC 8 cut(s) 668, 791, 1013, 1393, 1705, 1831, 1970, 2110
CspCI CAANNNNNGTGG 2 cut(s) 1757, 1792
CviQI GTAC 8 cut(s) 668, 791, 1013, 1393, 1705, 1831, 1970, 2110
DraIII CACNNNGTG 1 cut(s) 1302
DrdI GACNNNNNNGTC 2 cut(s) 590, 2534
DseDI GACNNNNNNGTC 2 cut(s) 590, 2534
EaeI YGGCCR 1 cut(s) 951
Eam1104I CTCTTC 3 cut(s) 46, 751, 1939
EarI CTCTTC 3 cut(s) 46, 751, 1939
Eco130I CCWWGG 4 cut(s) 512, 948, 1182, 1667
Eco147I AGGCCT 1 cut(s) 2720
Eco31I GGTCTC 2 cut(s) 26, 753
Eco47I GGWCC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 1430
Eco81I CCTNAGG 1 cut(s) 1750
Eco88I CYCGRG 2 cut(s) 816, 1653
EcoNI CCTNNNNNAGG 2 cut(s) 1631, 2390
EcoO109I RGGNCCY 2 cut(s) 516, 2396
EcoRI GAATTC 1 cut(s) 1922
EcoRII CCWGG 3 cut(s) 136, 2385, 2673
EcoT14I CCWWGG 4 cut(s) 512, 948, 1182, 1667
EcoT22I ATGCAT 2 cut(s) 871, 2353
ErhI CCWWGG 4 cut(s) 512, 948, 1182, 1667
FalI AAGNNNNNCTT 4 cut(s) 1399, 1431, 2754, 2786
FaqI GGGAC 2 cut(s) 502, 713
FauNDI CATATG 2 cut(s) 1561, 1687
FbaI TGATCA 2 cut(s) 721, 2557
FblI GTMKAC 1 cut(s) 921
Fnu4HI GCNGC 9 cut(s) 75, 459, 973, 1375, 1460, 2032, 2205, 2645, 2779
FokI GGATG 7 cut(s) 175, 974, 995, 1118, 1152, 1200, 1895
Fsp4HI GCNGC 9 cut(s) 75, 459, 973, 1375, 1460, 2032, 2205, 2645, 2779
GluI GCNGC 9 cut(s) 75, 459, 973, 1375, 1460, 2032, 2205, 2645, 2779
HaeIII GGCC 7 cut(s) 651, 953, 996, 1119, 1542, 2398, 2720
HapII CCGG 2 cut(s) 290, 2701
HgaI GACGC 1 cut(s) 2699
Hin1I GRCGYC 2 cut(s) 1877, 2804
HindIII AAGCTT 2 cut(s) 1413, 2795
HpaII CCGG 2 cut(s) 290, 2701
HphI GGTGA 8 cut(s) 202, 830, 1033, 1171, 2401, 2417, 2482, 2493
Hpy99I CGWCG 3 cut(s) 779, 1882, 2693
HpyCH4III ACNGT 9 cut(s) 358, 503, 834, 926, 1280, 1609, 1944, 2317, 2375
HpyCH4IV ACGT 5 cut(s) 718, 777, 1347, 1877, 2804
HpyF10VI GCNNNNNNNGC 8 cut(s) 467, 551, 1280, 1450, 1459, 1504, 1964, 2650
HpySE526I ACGT 5 cut(s) 718, 777, 1347, 1877, 2804
Hsp92I GRCGYC 2 cut(s) 1877, 2804
Ksp22I TGATCA 2 cut(s) 721, 2557
KspI CCGCGG 1 cut(s) 1378
LmnI GCTCC 7 cut(s) 508, 1435, 1713, 1802, 1928, 2159, 2243
Lsp1109I GCAGC 6 cut(s) 61, 445, 959, 1471, 2191, 2656
LweI GCATC 7 cut(s) 541, 952, 1130, 1440, 1967, 2338, 2360
MaeII ACGT 5 cut(s) 718, 777, 1347, 1877, 2804
MaeIII GTNAC 7 cut(s) 122, 307, 926, 1196, 2209, 2455, 2499
MfeI CAATTG 1 cut(s) 1338
MflI RGATCY 1 cut(s) 204
MlsI TGGCCA 1 cut(s) 953
MluNI TGGCCA 1 cut(s) 953
MlyI GAGTC 4 cut(s) 63, 130, 695, 1901
MmeI TCCRAC 5 cut(s) 1111, 1361, 1455, 1457, 1860
Mox20I TGGCCA 1 cut(s) 953
Mph1103I ATGCAT 2 cut(s) 871, 2353
MroXI GAANNNNTTC 1 cut(s) 1415
MscI TGGCCA 1 cut(s) 953
MseI TTAA 5 cut(s) 597, 977, 1632, 2340, 2583
MslI CAYNNNNRTG 1 cut(s) 305
Msp20I TGGCCA 1 cut(s) 953
MspA1I CMGCKG 1 cut(s) 1377
MspCI CTTAAG 1 cut(s) 1631
MspI CCGG 2 cut(s) 290, 2701
MspR9I CCNGG 4 cut(s) 138, 291, 2387, 2675
MunI CAATTG 1 cut(s) 1338
Mva1269I GAATGC 4 cut(s) 871, 1007, 1517, 2285
MvaI CCWGG 3 cut(s) 138, 2387, 2675
MvnI CGCG 1 cut(s) 1377
MwoI GCNNNNNNNGC 8 cut(s) 467, 551, 1280, 1450, 1459, 1504, 1964, 2650
NciI CCSGG 1 cut(s) 291
NcoI CCATGG 2 cut(s) 948, 1667
NdeI CATATG 2 cut(s) 1561, 1687
NlaIV GGNNCC 8 cut(s) 206, 230, 427, 510, 518, 1930, 2397, 2749
NmuCI GTSAC 6 cut(s) 122, 307, 926, 1196, 2455, 2499
NsiI ATGCAT 2 cut(s) 871, 2353
NspI RCATGY 4 cut(s) 934, 1100, 2537, 2567
PaeI GCATGC 2 cut(s) 1100, 2567
PagI TCATGA 4 cut(s) 348, 724, 1243, 2509
PaqCI CACCTGC 1 cut(s) 1727
PceI AGGCCT 1 cut(s) 2720
PciI ACATGT 2 cut(s) 930, 2533
PctI GAATGC 4 cut(s) 871, 1007, 1517, 2285
PdmI GAANNNNTTC 1 cut(s) 1415
PfeI GAWTC 6 cut(s) 1259, 1850, 2089, 2472, 2710, 2729
PkrI GCNGC 9 cut(s) 76, 460, 974, 1376, 1461, 2033, 2206, 2646, 2780
Ple19I CGATCG 1 cut(s) 674
PleI GAGTC 4 cut(s) 62, 129, 695, 1900
PpsI GAGTC 4 cut(s) 62, 129, 695, 1900
PpuMI RGGWCCY 1 cut(s) 516
PscI ACATGT 2 cut(s) 930, 2533
Psp5II RGGWCCY 1 cut(s) 516
Psp6I CCWGG 3 cut(s) 136, 2385, 2673
PspGI CCWGG 3 cut(s) 136, 2385, 2673
PspN4I GGNNCC 8 cut(s) 206, 230, 427, 510, 518, 1930, 2397, 2749
PspPI GGNCC 4 cut(s) 516, 1118, 1540, 2396
PspPPI RGGWCCY 1 cut(s) 516
PstNI CAGNNNCTG 2 cut(s) 1453, 2814
PsuI RGATCY 1 cut(s) 204
PvuI CGATCG 1 cut(s) 674
RsaI GTAC 8 cut(s) 669, 792, 1014, 1394, 1706, 1832, 1971, 2111
RsaNI GTAC 8 cut(s) 668, 791, 1013, 1393, 1705, 1831, 1970, 2110
RseI CAYNNNNRTG 1 cut(s) 305
SacII CCGCGG 1 cut(s) 1378
SaqAI TTAA 5 cut(s) 597, 977, 1632, 2340, 2583
SatI GCNGC 9 cut(s) 75, 459, 973, 1375, 1460, 2032, 2205, 2645, 2779
Sau96I GGNCC 4 cut(s) 516, 1118, 1540, 2396
ScaI AGTACT 1 cut(s) 1971
SchI GAGTC 4 cut(s) 63, 130, 695, 1901
ScrFI CCNGG 4 cut(s) 138, 291, 2387, 2675
SfaNI GCATC 7 cut(s) 541, 952, 1130, 1440, 1967, 2338, 2360
Sfr303I CCGCGG 1 cut(s) 1378
SgrBI CCGCGG 1 cut(s) 1378
SinI GGWCC 1 cut(s) 516
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 3 cut(s) 1631, 2367, 2654
SmoI CTYRAG 3 cut(s) 1631, 2367, 2654
SphI GCATGC 2 cut(s) 1100, 2567
SseBI AGGCCT 1 cut(s) 2720
SsiI CCGC 4 cut(s) 1375, 1377, 2031, 2779
SspI AATATT 1 cut(s) 892
StuI AGGCCT 1 cut(s) 2720
StyD4I CCNGG 4 cut(s) 136, 289, 2385, 2673
StyI CCWWGG 4 cut(s) 512, 948, 1182, 1667
TaaI ACNGT 9 cut(s) 358, 503, 834, 926, 1280, 1609, 1944, 2317, 2375
TaiI ACGT 5 cut(s) 721, 780, 1350, 1880, 2807
TaqI TCGA 7 cut(s) 630, 674, 774, 1818, 2171, 2268, 2448
TatI WGTACW 4 cut(s) 790, 1392, 1704, 1969
TauI GCSGC 3 cut(s) 1377, 2034, 2781
TfiI GAWTC 6 cut(s) 1259, 1850, 2089, 2472, 2710, 2729
Tru1I TTAA 5 cut(s) 597, 977, 1632, 2340, 2583
Tru9I TTAA 5 cut(s) 597, 977, 1632, 2340, 2583
TscAI CASTG 8 cut(s) 406, 482, 508, 1303, 1614, 1714, 1947, 2758
TseFI GTSAC 6 cut(s) 122, 307, 926, 1196, 2455, 2499
TseI GCWGC 6 cut(s) 74, 458, 972, 1459, 2204, 2644
Tsp45I GTSAC 6 cut(s) 122, 307, 926, 1196, 2455, 2499
TspGWI ACGGA 2 cut(s) 1939, 2806
TspRI CASTG 8 cut(s) 406, 482, 508, 1303, 1614, 1714, 1947, 2758
Vha464I CTTAAG 1 cut(s) 1631
VpaK11BI GGWCC 1 cut(s) 516
XagI CCTNNNNNAGG 2 cut(s) 1631, 2390
XapI RAATTY 7 cut(s) 1027, 1582, 1922, 1975, 2067, 2190, 2444
XceI RCATGY 4 cut(s) 934, 1100, 2537, 2567
XmiI GTMKAC 1 cut(s) 921
XmnI GAANNNNTTC 1 cut(s) 1415
ZraI GACGTC 2 cut(s) 1878, 2805
ZrmI AGTACT 1 cut(s) 1971
Zsp2I ATGCAT 2 cut(s) 871, 2353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.