Belongs to the glycosyltransferase 2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 782 |
| AccB1I | GGYRCC | 2 cut(s) | 1169, 1583 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 522, 1285 |
| AccBSI | CCGCTC | 1 cut(s) | 1154 |
| AccI | GTMKAC | 1 cut(s) | 843 |
| AciI | CCGC | 5 cut(s) | 380, 468, 1058, 1121, 1154 |
| AclWI | GGATC | 2 cut(s) | 257, 671 |
| AcoI | YGGCCR | 2 cut(s) | 1030, 1249 |
| AcyI | GRCGYC | 1 cut(s) | 503 |
| AfaI | GTAC | 5 cut(s) | 52, 158, 971, 1261, 1701 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 182, 373, 522, 1285, 1615 |
| AflIII | ACRYGT | 2 cut(s) | 846, 966 |
| AgsI | TTSAA | 7 cut(s) | 547, 554, 680, 886, 1127, 1218, 1496 |
| AhlI | ACTAGT | 1 cut(s) | 1115 |
| AleI | CACNNNNGTG | 1 cut(s) | 1581 |
| AluBI | AGCT | 4 cut(s) | 182, 691, 890, 1320 |
| AluI | AGCT | 4 cut(s) | 182, 691, 890, 1320 |
| Alw26I | GTCTC | 6 cut(s) | 510, 789, 856, 1031, 1202, 1540 |
| AlwI | GGATC | 2 cut(s) | 257, 671 |
| AoxI | GGCC | 4 cut(s) | 1030, 1155, 1249, 1445 |
| ApeKI | GCWGC | 1 cut(s) | 1317 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1180, 1212 |
| AspS9I | GGNCC | 1 cut(s) | 614 |
| AsuC2I | CCSGG | 1 cut(s) | 336 |
| AsuHPI | GGTGA | 2 cut(s) | 403, 555 |
| AvaII | GGWCC | 1 cut(s) | 614 |
| BaeGI | GKGCMC | 1 cut(s) | 1588 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1258, 1291 |
| BalI | TGGCCA | 2 cut(s) | 1032, 1251 |
| BanI | GGYRCC | 2 cut(s) | 1169, 1583 |
| BanII | GRGCYC | 1 cut(s) | 1621 |
| BauI | CACGAG | 1 cut(s) | 1034 |
| BbvI | GCAGC | 1 cut(s) | 1329 |
| BccI | CCATC | 8 cut(s) | 192, 439, 458, 782, 812, 1191, 1241, 1345 |
| BceAI | ACGGC | 1 cut(s) | 791 |
| BciVI | GTATCC | 2 cut(s) | 519, 1286 |
| BclI | TGATCA | 1 cut(s) | 757 |
| BcnI | CCSGG | 1 cut(s) | 336 |
| BcoDI | GTCTC | 6 cut(s) | 510, 789, 856, 1031, 1202, 1540 |
| BcuI | ACTAGT | 1 cut(s) | 1115 |
| BfaI | CTAG | 2 cut(s) | 1116, 1478 |
| BfmI | CTRYAG | 1 cut(s) | 360 |
| BfuI | GTATCC | 2 cut(s) | 519, 1286 |
| BisI | GCNGC | 3 cut(s) | 380, 1155, 1318 |
| BlsI | GCNGC | 3 cut(s) | 381, 1156, 1319 |
| BmcAI | AGTACT | 2 cut(s) | 52, 1261 |
| Bme1390I | CCNGG | 1 cut(s) | 336 |
| Bme18I | GGWCC | 1 cut(s) | 614 |
| BmgT120I | GGNCC | 1 cut(s) | 614 |
| BmiI | GGNNCC | 6 cut(s) | 378, 737, 895, 1171, 1585, 1620 |
| BmrFI | CCNGG | 1 cut(s) | 336 |
| BmsI | GCATC | 3 cut(s) | 812, 976, 1358 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1182, 1214 |
| BpmI | CTGGAG | 4 cut(s) | 204, 292, 912, 1605 |
| BpuEI | CTTGAG | 1 cut(s) | 281 |
| BpuMI | CCSGG | 1 cut(s) | 336 |
| Bsa29I | ATCGAT | 2 cut(s) | 666, 1311 |
| BsaHI | GRCGYC | 1 cut(s) | 503 |
| BsaI | GGTCTC | 1 cut(s) | 856 |
| BsaJI | CCNNGG | 4 cut(s) | 557, 807, 1675, 1695 |
| BsaWI | WCCGGW | 2 cut(s) | 565, 1172 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 182, 373, 522, 1285, 1615 |
| Bse1I | ACTGG | 4 cut(s) | 1033, 1134, 1396, 1622 |
| Bse3DI | GCAATG | 1 cut(s) | 940 |
| BseCI | ATCGAT | 2 cut(s) | 666, 1311 |
| BseDI | CCNNGG | 4 cut(s) | 557, 807, 1675, 1695 |
| BseGI | GGATG | 7 cut(s) | 171, 466, 827, 1024, 1054, 1181, 1373 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 182, 373, 522, 1285, 1615 |
| BseMI | GCAATG | 1 cut(s) | 940 |
| BseMII | CTCAG | 6 cut(s) | 216, 272, 426, 741, 902, 1503 |
| BseNI | ACTGG | 4 cut(s) | 1033, 1134, 1396, 1622 |
| BseRI | GAGGAG | 2 cut(s) | 338, 1650 |
| BseSI | GKGCMC | 1 cut(s) | 1588 |
| BseXI | GCAGC | 1 cut(s) | 1329 |
| BseYI | CCCAGC | 1 cut(s) | 1133 |
| BsgI | GTGCAG | 1 cut(s) | 794 |
| BshFI | GGCC | 4 cut(s) | 1032, 1157, 1251, 1447 |
| BshNI | GGYRCC | 2 cut(s) | 1169, 1583 |
| BshVI | ATCGAT | 2 cut(s) | 666, 1311 |
| BsiSI | CCGG | 3 cut(s) | 335, 566, 1173 |
| BslFI | GGGAC | 1 cut(s) | 1368 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 182, 373, 522, 1285, 1615 |
| BsmAI | GTCTC | 6 cut(s) | 510, 789, 856, 1031, 1202, 1540 |
| BsmBI | CGTCTC | 1 cut(s) | 510 |
| BsmFI | GGGAC | 1 cut(s) | 1368 |
| BsmI | GAATGC | 3 cut(s) | 586, 907, 1680 |
| BsnI | GGCC | 4 cut(s) | 1032, 1157, 1251, 1447 |
| Bso31I | GGTCTC | 1 cut(s) | 856 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1588, 1621 |
| Bsp1407I | TGTACA | 1 cut(s) | 156 |
| Bsp143I | GATC | 3 cut(s) | 249, 663, 757 |
| Bsp19I | CCATGG | 1 cut(s) | 1675 |
| BspACI | CCGC | 5 cut(s) | 380, 468, 1058, 1121, 1154 |
| BspANI | GGCC | 4 cut(s) | 1032, 1157, 1251, 1447 |
| BspCNI | CTCAG | 6 cut(s) | 217, 271, 427, 740, 903, 1504 |
| BspDI | ATCGAT | 2 cut(s) | 666, 1311 |
| BspLI | GGNNCC | 6 cut(s) | 378, 737, 895, 1171, 1585, 1620 |
| BspMAI | CTGCAG | 1 cut(s) | 364 |
| BspPI | GGATC | 2 cut(s) | 257, 671 |
| BspT107I | GGYRCC | 2 cut(s) | 1169, 1583 |
| BspTNI | GGTCTC | 1 cut(s) | 856 |
| BsrBI | CCGCTC | 1 cut(s) | 1154 |
| BsrDI | GCAATG | 1 cut(s) | 940 |
| BsrGI | TGTACA | 1 cut(s) | 156 |
| BsrI | ACTGG | 4 cut(s) | 1033, 1134, 1396, 1622 |
| BssECI | CCNNGG | 4 cut(s) | 557, 807, 1675, 1695 |
| BssMI | GATC | 3 cut(s) | 249, 663, 757 |
| BssNI | GRCGYC | 1 cut(s) | 503 |
| BssSI | CACGAG | 1 cut(s) | 1034 |
| BssT1I | CCWWGG | 3 cut(s) | 557, 1675, 1695 |
| Bst2BI | CACGAG | 1 cut(s) | 1034 |
| Bst4CI | ACNGT | 3 cut(s) | 403, 477, 962 |
| Bst6I | CTCTTC | 6 cut(s) | 13, 36, 69, 787, 1183, 1250 |
| BstACI | GRCGYC | 1 cut(s) | 503 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 980 |
| BstAUI | TGTACA | 1 cut(s) | 156 |
| BstC8I | GCNNGC | 2 cut(s) | 1105, 1670 |
| BstDEI | CTNAG | 6 cut(s) | 225, 258, 435, 727, 911, 1512 |
| BstDSI | CCRYGG | 2 cut(s) | 807, 1675 |
| BstEII | GGTNACC | 1 cut(s) | 561 |
| BstF5I | GGATG | 7 cut(s) | 171, 466, 827, 1024, 1054, 1181, 1373 |
| BstKTI | GATC | 3 cut(s) | 252, 666, 760 |
| BstMAI | GTCTC | 6 cut(s) | 510, 789, 856, 1031, 1202, 1540 |
| BstMBI | GATC | 3 cut(s) | 249, 663, 757 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 902, 980, 1057 |
| BstNSI | RCATGY | 4 cut(s) | 850, 970, 1107, 1522 |
| BstPI | GGTNACC | 1 cut(s) | 561 |
| BstSCI | CCNGG | 1 cut(s) | 334 |
| BstSFI | CTRYAG | 1 cut(s) | 360 |
| BstSLI | GKGCMC | 1 cut(s) | 1588 |
| BstV1I | GCAGC | 1 cut(s) | 1329 |
| Bsu15I | ATCGAT | 2 cut(s) | 666, 1311 |
| BsuI | GTATCC | 2 cut(s) | 519, 1286 |
| BsuRI | GGCC | 4 cut(s) | 1032, 1157, 1251, 1447 |
| BsuTUI | ATCGAT | 2 cut(s) | 666, 1311 |
| BtgI | CCRYGG | 2 cut(s) | 807, 1675 |
| BtsCI | GGATG | 7 cut(s) | 171, 466, 827, 1024, 1054, 1181, 1373 |
| BtsI | GCAGTG | 2 cut(s) | 357, 905 |
| BtsIMutI | CAGTG | 3 cut(s) | 357, 399, 905 |
| Cac8I | GCNNGC | 2 cut(s) | 1105, 1670 |
| Cfr13I | GGNCC | 1 cut(s) | 614 |
| ClaI | ATCGAT | 2 cut(s) | 666, 1311 |
| CseI | GACGC | 1 cut(s) | 492 |
| Csp6I | GTAC | 5 cut(s) | 51, 157, 970, 1260, 1700 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 789, 824, 1487, 1522 |
| CviAII | CATG | 8 cut(s) | 220, 847, 898, 967, 1104, 1519, 1565, 1676 |
| CviQI | GTAC | 5 cut(s) | 51, 157, 970, 1260, 1700 |
| DdeI | CTNAG | 6 cut(s) | 225, 258, 435, 727, 911, 1512 |
| DpnI | GATC | 3 cut(s) | 251, 665, 759 |
| DpnII | GATC | 3 cut(s) | 249, 663, 757 |
| EaeI | YGGCCR | 2 cut(s) | 1030, 1249 |
| Eam1104I | CTCTTC | 6 cut(s) | 13, 36, 69, 787, 1183, 1250 |
| EarI | CTCTTC | 6 cut(s) | 13, 36, 69, 787, 1183, 1250 |
| Eco130I | CCWWGG | 3 cut(s) | 557, 1675, 1695 |
| Eco147I | AGGCCT | 1 cut(s) | 1447 |
| Eco24I | GRGCYC | 1 cut(s) | 1621 |
| Eco31I | GGTCTC | 1 cut(s) | 856 |
| Eco47I | GGWCC | 1 cut(s) | 614 |
| Eco91I | GGTNACC | 1 cut(s) | 561 |
| EcoO65I | GGTNACC | 1 cut(s) | 561 |
| EcoT14I | CCWWGG | 3 cut(s) | 557, 1675, 1695 |
| EcoT22I | ATGCAT | 1 cut(s) | 1105 |
| EcoT38I | GRGCYC | 1 cut(s) | 1621 |
| ErhI | CCWWGG | 3 cut(s) | 557, 1675, 1695 |
| Esp3I | CGTCTC | 1 cut(s) | 510 |
| FaeI | CATG | 8 cut(s) | 223, 850, 901, 970, 1107, 1522, 1568, 1679 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1562, 1594 |
| FaqI | GGGAC | 1 cut(s) | 1368 |
| FatI | CATG | 8 cut(s) | 219, 846, 897, 966, 1103, 1518, 1564, 1675 |
| FbaI | TGATCA | 1 cut(s) | 757 |
| FblI | GTMKAC | 1 cut(s) | 843 |
| Fnu4HI | GCNGC | 3 cut(s) | 380, 1155, 1318 |
| FokI | GGATG | 7 cut(s) | 158, 473, 834, 1031, 1041, 1188, 1380 |
| FriOI | GRGCYC | 1 cut(s) | 1621 |
| Fsp4HI | GCNGC | 3 cut(s) | 380, 1155, 1318 |
| FspBI | CTAG | 2 cut(s) | 1116, 1478 |
| GluI | GCNGC | 3 cut(s) | 380, 1155, 1318 |
| GsaI | CCCAGC | 1 cut(s) | 1137 |
| GsuI | CTGGAG | 4 cut(s) | 204, 292, 912, 1605 |
| HaeIII | GGCC | 4 cut(s) | 1032, 1157, 1251, 1447 |
| HapII | CCGG | 3 cut(s) | 335, 566, 1173 |
| HgaI | GACGC | 1 cut(s) | 492 |
| Hin1I | GRCGYC | 1 cut(s) | 503 |
| Hin1II | CATG | 8 cut(s) | 223, 850, 901, 970, 1107, 1522, 1568, 1679 |
| HincII | GTYRAC | 1 cut(s) | 290 |
| HindII | GTYRAC | 1 cut(s) | 290 |
| HinfI | GANTC | 4 cut(s) | 306, 668, 709, 1292 |
| HpaII | CCGG | 3 cut(s) | 335, 566, 1173 |
| HphI | GGTGA | 2 cut(s) | 403, 555 |
| Hpy166II | GTNNAC | 6 cut(s) | 157, 290, 473, 480, 811, 844 |
| Hpy188I | TCNGA | 9 cut(s) | 436, 460, 592, 663, 730, 925, 1513, 1527, 1540 |
| Hpy188III | TCNNGA | 5 cut(s) | 175, 298, 617, 852, 1439 |
| Hpy8I | GTNNAC | 6 cut(s) | 157, 290, 473, 480, 811, 844 |
| HpyAV | CCTTC | 8 cut(s) | 4, 8, 17, 23, 29, 952, 1458, 1619 |
| HpyCH4III | ACNGT | 3 cut(s) | 403, 477, 962 |
| HpyCH4IV | ACGT | 1 cut(s) | 594 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 902, 980, 1057 |
| HpyF3I | CTNAG | 6 cut(s) | 225, 258, 435, 727, 911, 1512 |
| HpySE526I | ACGT | 1 cut(s) | 594 |
| Hsp92I | GRCGYC | 1 cut(s) | 503 |
| Hsp92II | CATG | 8 cut(s) | 223, 850, 901, 970, 1107, 1522, 1568, 1679 |
| Ksp22I | TGATCA | 1 cut(s) | 757 |
| Kzo9I | GATC | 3 cut(s) | 249, 663, 757 |
| LmnI | GCTCC | 7 cut(s) | 137, 376, 893, 1151, 1471, 1624, 1677 |
| Lsp1109I | GCAGC | 1 cut(s) | 1329 |
| LweI | GCATC | 3 cut(s) | 812, 976, 1358 |
| MaeI | CTAG | 2 cut(s) | 1116, 1478 |
| MaeII | ACGT | 1 cut(s) | 594 |
| MaeIII | GTNAC | 6 cut(s) | 277, 561, 962, 1111, 1223, 1387 |
| MalI | GATC | 3 cut(s) | 251, 665, 759 |
| MbiI | CCGCTC | 1 cut(s) | 1154 |
| MboI | GATC | 3 cut(s) | 249, 663, 757 |
| MfeI | CAATTG | 1 cut(s) | 768 |
| MhlI | GDGCHC | 2 cut(s) | 1588, 1621 |
| MlsI | TGGCCA | 2 cut(s) | 1032, 1251 |
| MluCI | AATT | 3 cut(s) | 424, 768, 1283 |
| MluNI | TGGCCA | 2 cut(s) | 1032, 1251 |
| MlyI | GAGTC | 1 cut(s) | 300 |
| MmeI | TCCRAC | 1 cut(s) | 1397 |
| Mox20I | TGGCCA | 2 cut(s) | 1032, 1251 |
| Mph1103I | ATGCAT | 1 cut(s) | 1105 |
| MscI | TGGCCA | 2 cut(s) | 1032, 1251 |
| MseI | TTAA | 4 cut(s) | 396, 597, 741, 1711 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1581 |
| Msp20I | TGGCCA | 2 cut(s) | 1032, 1251 |
| MspI | CCGG | 3 cut(s) | 335, 566, 1173 |
| MspR9I | CCNGG | 1 cut(s) | 336 |
| MunI | CAATTG | 1 cut(s) | 768 |
| Mva1269I | GAATGC | 3 cut(s) | 586, 907, 1680 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 902, 980, 1057 |
| NciI | CCSGG | 1 cut(s) | 336 |
| NcoI | CCATGG | 1 cut(s) | 1675 |
| NdeII | GATC | 3 cut(s) | 249, 663, 757 |
| NlaIII | CATG | 8 cut(s) | 223, 850, 901, 970, 1107, 1522, 1568, 1679 |
| NlaIV | GGNNCC | 6 cut(s) | 378, 737, 895, 1171, 1585, 1620 |
| NmuCI | GTSAC | 3 cut(s) | 277, 561, 962 |
| NsiI | ATGCAT | 1 cut(s) | 1105 |
| NspI | RCATGY | 4 cut(s) | 850, 970, 1107, 1522 |
| OliI | CACNNNNGTG | 1 cut(s) | 1581 |
| PaeI | GCATGC | 1 cut(s) | 1107 |
| PceI | AGGCCT | 1 cut(s) | 1447 |
| PciI | ACATGT | 2 cut(s) | 846, 966 |
| PctI | GAATGC | 3 cut(s) | 586, 907, 1680 |
| PfeI | GAWTC | 3 cut(s) | 668, 709, 1292 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 522, 1285 |
| PkrI | GCNGC | 3 cut(s) | 381, 1156, 1319 |
| PleI | GAGTC | 1 cut(s) | 300 |
| PpsI | GAGTC | 1 cut(s) | 300 |
| PscI | ACATGT | 2 cut(s) | 846, 966 |
| PsiI | TTATAA | 1 cut(s) | 782 |
| PspEI | GGTNACC | 1 cut(s) | 561 |
| PspFI | CCCAGC | 1 cut(s) | 1133 |
| PspN4I | GGNNCC | 6 cut(s) | 378, 737, 895, 1171, 1585, 1620 |
| PspPI | GGNCC | 1 cut(s) | 614 |
| PstI | CTGCAG | 1 cut(s) | 364 |
| RsaI | GTAC | 5 cut(s) | 52, 158, 971, 1261, 1701 |
| RsaNI | GTAC | 5 cut(s) | 51, 157, 970, 1260, 1700 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1581 |
| SaqAI | TTAA | 4 cut(s) | 396, 597, 741, 1711 |
| SatI | GCNGC | 3 cut(s) | 380, 1155, 1318 |
| Sau3AI | GATC | 3 cut(s) | 249, 663, 757 |
| Sau96I | GGNCC | 1 cut(s) | 614 |
| ScaI | AGTACT | 2 cut(s) | 52, 1261 |
| SchI | GAGTC | 1 cut(s) | 300 |
| ScrFI | CCNGG | 1 cut(s) | 336 |
| SduI | GDGCHC | 2 cut(s) | 1588, 1621 |
| SfaNI | GCATC | 3 cut(s) | 812, 976, 1358 |
| SfcI | CTRYAG | 1 cut(s) | 360 |
| SinI | GGWCC | 1 cut(s) | 614 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1581 |
| SmlI | CTYRAG | 1 cut(s) | 296 |
| SmoI | CTYRAG | 1 cut(s) | 296 |
| SpeI | ACTAGT | 1 cut(s) | 1115 |
| SphI | GCATGC | 1 cut(s) | 1107 |
| Sse9I | AATT | 3 cut(s) | 424, 768, 1283 |
| SseBI | AGGCCT | 1 cut(s) | 1447 |
| SsiI | CCGC | 5 cut(s) | 380, 468, 1058, 1121, 1154 |
| SspMI | CTAG | 2 cut(s) | 1116, 1478 |
| StuI | AGGCCT | 1 cut(s) | 1447 |
| StyD4I | CCNGG | 1 cut(s) | 334 |
| StyI | CCWWGG | 3 cut(s) | 557, 1675, 1695 |
| TaaI | ACNGT | 3 cut(s) | 403, 477, 962 |
| TaiI | ACGT | 1 cut(s) | 597 |
| TaqI | TCGA | 4 cut(s) | 666, 1186, 1311, 1377 |
| TasI | AATT | 3 cut(s) | 424, 768, 1283 |
| TatI | WGTACW | 3 cut(s) | 50, 156, 1259 |
| TauI | GCSGC | 2 cut(s) | 382, 1157 |
| TfiI | GAWTC | 3 cut(s) | 668, 709, 1292 |
| Tru1I | TTAA | 4 cut(s) | 396, 597, 741, 1711 |
| Tru9I | TTAA | 4 cut(s) | 396, 597, 741, 1711 |
| TscAI | CASTG | 3 cut(s) | 364, 406, 912 |
| TseFI | GTSAC | 3 cut(s) | 277, 561, 962 |
| TseI | GCWGC | 1 cut(s) | 1317 |
| Tsp45I | GTSAC | 3 cut(s) | 277, 561, 962 |
| TspDTI | ATGAA | 8 cut(s) | 660, 668, 914, 934, 1035, 1338, 1543, 1617 |
| TspRI | CASTG | 3 cut(s) | 364, 406, 912 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 522, 1285 |
| VpaK11BI | GGWCC | 1 cut(s) | 614 |
| XceI | RCATGY | 4 cut(s) | 850, 970, 1107, 1522 |
| XmiI | GTMKAC | 1 cut(s) | 843 |
| XspI | CTAG | 2 cut(s) | 1116, 1478 |
| ZrmI | AGTACT | 2 cut(s) | 52, 1261 |
| Zsp2I | ATGCAT | 1 cut(s) | 1105 |