Rroxscaffold_1G00005210

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000273
Physical Location & Seq
Reverse (-)
7048445 .. 7050939
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005210.1

Sequence Viewer

Length: 1764 bp
ATGATGGTCATGAACACCGTTTTGTCAGTCATGGCTTATGATTACCCAACCGAGAAGATCAGCGTGTATCTATCCGATGATGGCGGTTCCGAGCTTACATACTACGCACTCTTGGAGGCTGCTGAATTTGCTAAGCAGTGGATACCATACTGCAAGAAATACAGTGTGGAGCCAAGGTCCCCAGTTGCTTACTTTGTCTCAATAGCTGCTGATGCAGTTGGTGTTGATGGTAACAAGGCTAATGACTTGGCTGTCATTAAGAAATCATACAAGAACATGGAGACTAAAATCGAAAATGCAGTCAAATTAGGCCACATTCCAGAGAAAGTACGATCGAAACAGAAAGGCTTTTCTCAATGGGACTCACATTTGTCTAAACGTGATCATGACACCTATCTTCAAGTAGTAATTGATGGAAGAGACCCAAAGGCAATCGATGTTGAAGGACTTGTACTGCCAACTTTAGTGTATTTAGCTCGGGAGAAAAGACCACAGTATCATCATAACTTCAAAGCTGGCTCTATGAATGCATTGATTAGGGTTTCTTCAAATATTAGCAACGGGAAGGTCATTCTAAATGTAGACTGTGACATGTATTCAAACAATTCCATGGCCATAAGGGATGCACTTTGCTGCTTAATGGATGAAGAAAAAGGCCATGAAGTTGCATTCATACAATTCCCACAAAATTTTGAAAACCTCACCAAGAATGAATTATATATTCCATTCATGCGAACAATCAGCGAGGTGGAATTGCATGCTTTGGATGGTTTTGATGGCCCTTTTTACGTTGGAAGTGGATGCTTCCACAGAAGGGATACTCTTTGTGGAAGGAAGTTCACCAAGGGATGTAAGAGTGACATGAAATGGGAGATCAGTAGAAAAAGAGAAGAAACTACAATTCATGAATTGGAAGAAGATTCAAAAGTTCTTGCAAACTGTGCATACGAGCAAAACACTGAGTGGGGAAAAGAGATGGGTTTGAAATATGGTTGTGCAATTGAAGACGTGTTCACGGGGTTATCAATCCAATGCCGCGGTTGGAGATCGGTGTACTTGAACCCAACAAGGAAAGCTTTCTTAGGAGTAGCTCCAACGACATTGCTTCAGATGCTGGTGCAGCAAAAGAGATGGTCGGAAGGTAGTTTCCAGATTTTGCTTTCACGGCACAATCCTGCATTCTCTGATCAGATTAGCTTGGGCCAAAAACTTGGATACGCATATGTCGGGGCTTGGTCTATCAATTTCCTACCAACCCTATTTTACACAGTGATCCCTTCCCTTTACCTCCTTAAGGGCATTTCCTTATTTCCTCGGGTTTCAAGTCCATGGATCATACCATTTGCATATGTGATAATTGCCAAGTACACTGGGAGCTTTTTGGAGTTTCTATGGGCAGGTGGCTCAGCCTTAGCCAAGACGTCGGACGAAGATGAGTCGCAACGATATGAGAAAGAGATTATGGAATTCGGAGCCTCTTCTCCACTGTTTACCGTATTGGCATCACTTGCAGTACTAAATTTGTATTGCTTTGCTGGTTTTGTGAAGGAAGCAATCAGTCTGGGATCAAAGGGAGGCATTGCAGAAGTTTACGATACAATGGCTTTGCAAATTCTACTATGTTGGATTTTGATTCTCATCAACCTGCCTTTGTACCAAGCCCTATATCTAAGGAAGGACAAGGGGAAGTTGCCAAGCTCCATAGCTCTGAAGTCGATGGCATTTGGTGTCTTTGGTTTAACATGTTTCAATTTTTCGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

587

Amino Acids

66.13

Weight (kDa)

7.12

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulose_synt PF03552 2 - 282 3.4e-82 Cellulose synthase
Cellulose_synt PF03552 299 - 547 2.2e-34 Cellulose synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55850
fragaria_vesca FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42770 FvH4_3g42800 FvH4_3g42810 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42900
malus_domestica MD03G1028800.v1.1 MD03G1028900.v1.1 MD03G1029000.v1.1 MD03G1029100.v1.1
prunus_persica Prupe.6G024800_v2.0.a1 Prupe.6G024900_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025100_v2.0.a1 Prupe.6G025100_v2.0.a1
pyrus_communis pycom01g03430 pycom03g02330 pycom03g02340 pycom03g02360 pycom03g02380 pycom03g02400 pycom03g02410 pycom05g13920
rosa_chinensis RchiOBHm_Chr5g0076821 RchiOBHm_Chr5g0076831 RchiOBHm_Chr5g0076851 RchiOBHm_Chr5g0076871 RchiOBHm_Chr5g0076891 RchiOBHm_Chr5g0076901 RchiOBHm_Chr5g0076911 RchiOBHm_Chr5g0076921
rosa_laevigata RLG00000036625 RLG00000036626 RLG00000036627 RLG00000036630 RLG00000036632 RLG00000036634 RLG00000036635
rosa_multiflora Rmu_co8084432.1_g000001 Rmu_co8208886.1_g000001 Rmu_co8325177.1_g000001 Rmu_sc0003799.1_g000001 Rmu_sc0003799.1_g000002 Rmu_sc0003799.1_g000006 Rmu_sc0003799.1_g000009 Rmu_sc0004655.1_g000002 Rmu_sc0004655.1_g000014 Rmu_sc0004655.1_g000015 Rmu_sc0007021.1_g000003 Rmu_sc0007021.1_g000013 Rmu_sc0007021.1_g000014 Rmu_sc0008244.1_g000002 Rmu_sc0013263.1_g000004 Rmu_sc0014402.1_g000001 Rmu_ssc0000184.1_g000003 Rmu_ssc0000184.1_g000004 Rmu_ssc0000184.1_g000005 Rmu_ssc0000184.1_g000006 Rmu_ssc0000184.1_g000014 Rmu_ssc0000184.1_g000021
rosa_roxburghii Rroxscaffold_1G00005210 Rroxscaffold_1G00005220 Rroxscaffold_1G00005250 Rroxscaffold_1G00005280 Rroxscaffold_1G00005300 Rroxscaffold_1G00005310
rosa_rugosa Rorug05G0449200 Rorug05G0449300 Rorug05G0449300 Rorug05G0449300 Rorug05G0449500 Rorug05G0449600
rosa_samantha Rh5AG503600 Rh5AG503700 Rh5AG503900 Rh5AG504000 Rh5AG504100 Rh5BG525000 Rh5BG525200 Rh5BG525300 Rh5BG525500 Rh5BG525700 Rh5BG525900 Rh5BG526000 Rh5CG548600 Rh5CG548800 Rh5CG548900 Rh5CG549100 Rh5CG549300 Rh5CG549500 Rh5DG537300 Rh5DG537400 Rh5DG537500 Rh5DG537700 Rh5DG537900 Rh5DG538000 Rh5DG544100
rosa_wichuraiana Rw0G009920 Rw5G046720 Rw5G046730 Rw5G047200 Rw5G047210 Rw5G047240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1388
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 1424
Acc36I ACCTGC 2 cut(s) 1388, 1653
AccI GTMKAC 1 cut(s) 582
AccII CGCG 1 cut(s) 1038
AciI CCGC 3 cut(s) 84, 1036, 1038
AclWI GGATC 3 cut(s) 1267, 1340, 1573
AcoI YGGCCR 1 cut(s) 612
AcsI RAATTY 5 cut(s) 125, 688, 1466, 1519, 1611
AcuI CTGAAG 2 cut(s) 1091, 1730
AcyI GRCGYC 1 cut(s) 1421
AdeI CACNNNGTG 1 cut(s) 963
AfaI GTAC 6 cut(s) 330, 453, 1055, 1367, 1515, 1655
AfiI CCNNNNNNNGG 2 cut(s) 814, 1294
AflII CTTAAG 1 cut(s) 1292
AflIII ACRYGT 3 cut(s) 591, 1008, 1742
AjiI CACGTC 1 cut(s) 1009
Alw26I GTCTC 3 cut(s) 202, 275, 414
AlwI GGATC 3 cut(s) 1267, 1340, 1573
AlwNI CAGNNNCTG 1 cut(s) 1114
Ama87I CYCGRG 2 cut(s) 477, 1314
AoxI GGCC 5 cut(s) 310, 612, 655, 778, 1201
ApeKI GCWGC 4 cut(s) 119, 206, 633, 1120
ApoI RAATTY 5 cut(s) 125, 688, 1466, 1519, 1611
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
Asp700I GAANNNNTTC 1 cut(s) 1076
AspS9I GGNCC 3 cut(s) 177, 779, 1201
AsuHPI GGTGA 2 cut(s) 694, 832
AvaI CYCGRG 2 cut(s) 477, 1314
AvaII GGWCC 1 cut(s) 177
BaeI ACNNNNGTAYC 2 cut(s) 479, 512
BalI TGGCCA 1 cut(s) 614
BarI GAAGNNNNNNTAC 2 cut(s) 435, 467
BbsI GAAGAC 1 cut(s) 1011
BbvI GCAGC 4 cut(s) 106, 193, 620, 1132
BccI CCATC 8 cut(s) 74, 221, 407, 761, 770, 970, 1125, 1711
BceAI ACGGC 1 cut(s) 1181
BciVI GTATCC 3 cut(s) 135, 811, 1208
BclI TGATCA 2 cut(s) 382, 1186
BcoDI GTCTC 3 cut(s) 202, 275, 414
BfrI CTTAAG 1 cut(s) 1292
BfuAI ACCTGC 2 cut(s) 1388, 1653
BfuI GTATCC 3 cut(s) 135, 811, 1208
BisI GCNGC 5 cut(s) 120, 207, 634, 1036, 1121
BlpI GCTNAGC 2 cut(s) 132, 1405
BlsI GCNGC 5 cut(s) 121, 208, 635, 1037, 1122
BmcAI AGTACT 1 cut(s) 1515
Bme18I GGWCC 1 cut(s) 177
BmeT110I CYCGRG 2 cut(s) 477, 1314
BmgBI CACGTC 1 cut(s) 1009
BmgT120I GGNCC 3 cut(s) 177, 779, 1201
BmiI GGNNCC 4 cut(s) 88, 171, 179, 1474
BmrI ACTGGG 2 cut(s) 176, 1380
BmsI GCATC 5 cut(s) 202, 613, 791, 1101, 1511
BmuI ACTGGG 2 cut(s) 176, 1380
BpiI GAAGAC 1 cut(s) 1011
Bpu10I CCTNAGC 1 cut(s) 1411
Bpu1102I GCTNAGC 2 cut(s) 132, 1405
Bsa29I ATCGAT 1 cut(s) 435
BsaBI GATNNNNATC 1 cut(s) 1637
BsaHI GRCGYC 1 cut(s) 1421
BsaI GGTCTC 1 cut(s) 414
BsaJI CCNNGG 6 cut(s) 173, 609, 843, 1036, 1313, 1328
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 814, 1294
Bse1I ACTGG 2 cut(s) 182, 1375
Bse3DI GCAATG 2 cut(s) 1100, 1578
Bse8I GATNNNNATC 1 cut(s) 1637
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 6 cut(s) 173, 609, 843, 1036, 1313, 1328
BseGI GGATG 5 cut(s) 628, 649, 772, 806, 854
BseJI GATNNNNATC 1 cut(s) 1637
BseLI CCNNNNNNNGG 2 cut(s) 814, 1294
BseMI GCAATG 2 cut(s) 1100, 1578
BseMII CTCAG 2 cut(s) 951, 1419
BseNI ACTGG 2 cut(s) 182, 1375
BseXI GCAGC 4 cut(s) 106, 193, 620, 1132
BsgI GTGCAG 1 cut(s) 1139
Bsh1236I CGCG 1 cut(s) 1038
Bsh1285I CGRYCG 1 cut(s) 335
BshFI GGCC 5 cut(s) 312, 614, 657, 780, 1203
BshVI ATCGAT 1 cut(s) 435
BsiEI CGRYCG 1 cut(s) 335
BsiHKCI CYCGRG 2 cut(s) 477, 1314
BslFI GGGAC 2 cut(s) 163, 374
BslI CCNNNNNNNGG 2 cut(s) 814, 1294
BsmAI GTCTC 3 cut(s) 202, 275, 414
BsmFI GGGAC 2 cut(s) 163, 374
BsmI GAATGC 3 cut(s) 532, 668, 1178
BsnI GGCC 5 cut(s) 312, 614, 657, 780, 1203
Bso31I GGTCTC 1 cut(s) 414
BsoBI CYCGRG 2 cut(s) 477, 1314
Bsp143I GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
Bsp1720I GCTNAGC 2 cut(s) 132, 1405
Bsp19I CCATGG 2 cut(s) 609, 1328
BspACI CCGC 3 cut(s) 84, 1036, 1038
BspANI GGCC 5 cut(s) 312, 614, 657, 780, 1203
BspCNI CTCAG 2 cut(s) 952, 1418
BspDI ATCGAT 1 cut(s) 435
BspFNI CGCG 1 cut(s) 1038
BspHI TCATGA 3 cut(s) 9, 385, 904
BspLI GGNNCC 4 cut(s) 88, 171, 179, 1474
BspMI ACCTGC 2 cut(s) 1388, 1653
BspPI GGATC 3 cut(s) 1267, 1340, 1573
BspTI CTTAAG 1 cut(s) 1292
BspTNI GGTCTC 1 cut(s) 414
BsrDI GCAATG 2 cut(s) 1100, 1578
BsrI ACTGG 2 cut(s) 182, 1375
BssECI CCNNGG 6 cut(s) 173, 609, 843, 1036, 1313, 1328
BssMI GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
BssNI GRCGYC 1 cut(s) 1421
BssT1I CCWWGG 4 cut(s) 173, 609, 843, 1328
Bst4CI ACNGT 8 cut(s) 19, 164, 495, 587, 941, 1270, 1488, 1495
Bst6I CTCTTC 2 cut(s) 412, 1483
BstACI GRCGYC 1 cut(s) 1421
BstAFI CTTAAG 1 cut(s) 1292
BstAPI GCANNNNNTGC 2 cut(s) 941, 1508
BstC8I GCNNGC 2 cut(s) 517, 759
BstDEI CTNAG 6 cut(s) 132, 960, 1081, 1405, 1411, 1670
BstDSI CCRYGG 3 cut(s) 609, 1036, 1328
BstENI CCTNNNNNAGG 1 cut(s) 1292
BstF5I GGATG 5 cut(s) 628, 649, 772, 806, 854
BstFNI CGCG 1 cut(s) 1038
BstKTI GATC 9 cut(s) 60, 335, 385, 876, 1049, 1189, 1275, 1335, 1568
BstMAI GTCTC 3 cut(s) 202, 275, 414
BstMBI GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
BstMCI CGRYCG 1 cut(s) 335
BstMWI GCNNNNNNNGC 7 cut(s) 128, 212, 941, 1111, 1120, 1165, 1508
BstNSI RCATGY 3 cut(s) 595, 761, 1746
BstUI CGCG 1 cut(s) 1038
BstV1I GCAGC 4 cut(s) 106, 193, 620, 1132
BstV2I GAAGAC 1 cut(s) 1011
BstXI CCANNNNNNTGG 1 cut(s) 1211
Bsu15I ATCGAT 1 cut(s) 435
BsuI GTATCC 3 cut(s) 135, 811, 1208
BsuRI GGCC 5 cut(s) 312, 614, 657, 780, 1203
BsuTUI ATCGAT 1 cut(s) 435
BtgI CCRYGG 3 cut(s) 609, 1036, 1328
BtrI CACGTC 1 cut(s) 1009
BtsCI GGATG 5 cut(s) 628, 649, 772, 806, 854
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 6 cut(s) 143, 169, 957, 1275, 1368, 1484
BveI ACCTGC 2 cut(s) 1388, 1653
Cac8I GCNNGC 2 cut(s) 517, 759
CaiI CAGNNNCTG 1 cut(s) 1114
CciI TCATGA 3 cut(s) 9, 385, 904
Cfr13I GGNCC 3 cut(s) 177, 779, 1201
Cfr42I CCGCGG 1 cut(s) 1039
ClaI ATCGAT 1 cut(s) 435
Csp6I GTAC 6 cut(s) 329, 452, 1054, 1366, 1514, 1654
CviQI GTAC 6 cut(s) 329, 452, 1054, 1366, 1514, 1654
DdeI CTNAG 6 cut(s) 132, 960, 1081, 1405, 1411, 1670
DpnI GATC 9 cut(s) 59, 334, 384, 875, 1048, 1188, 1274, 1334, 1567
DpnII GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
DraIII CACNNNGTG 1 cut(s) 963
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 2 cut(s) 412, 1483
EarI CTCTTC 2 cut(s) 412, 1483
Eco130I CCWWGG 4 cut(s) 173, 609, 843, 1328
Eco31I GGTCTC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 1091, 1730
Eco88I CYCGRG 2 cut(s) 477, 1314
EcoNI CCTNNNNNAGG 1 cut(s) 1292
EcoO109I RGGNCCY 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 1466
EcoT14I CCWWGG 4 cut(s) 173, 609, 843, 1328
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 4 cut(s) 173, 609, 843, 1328
FalI AAGNNNNNCTT 2 cut(s) 1060, 1092
FaqI GGGAC 2 cut(s) 163, 374
FauNDI CATATG 2 cut(s) 1222, 1348
FbaI TGATCA 2 cut(s) 382, 1186
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 5 cut(s) 120, 207, 634, 1036, 1121
FokI GGATG 5 cut(s) 635, 656, 779, 813, 861
Fsp4HI GCNGC 5 cut(s) 120, 207, 634, 1036, 1121
GluI GCNGC 5 cut(s) 120, 207, 634, 1036, 1121
HaeIII GGCC 5 cut(s) 312, 614, 657, 780, 1203
Hin1I GRCGYC 1 cut(s) 1421
HindIII AAGCTT 1 cut(s) 1074
HinfI GANTC 4 cut(s) 362, 920, 1436, 1633
HphI GGTGA 2 cut(s) 694, 832
Hpy166II GTNNAC 7 cut(s) 583, 840, 1014, 1054, 1368, 1491, 1591
Hpy188I TCNGA 9 cut(s) 76, 91, 1110, 1138, 1186, 1191, 1426, 1472, 1710
Hpy188III TCNNGA 6 cut(s) 10, 320, 386, 479, 905, 1150
Hpy8I GTNNAC 7 cut(s) 583, 840, 1014, 1054, 1368, 1491, 1591
Hpy99I CGWCG 1 cut(s) 1426
HpyAV CCTTC 8 cut(s) 437, 559, 807, 825, 1133, 1287, 1540, 1669
HpyCH4III ACNGT 8 cut(s) 19, 164, 495, 587, 941, 1270, 1488, 1495
HpyCH4IV ACGT 4 cut(s) 379, 789, 1008, 1421
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 212, 941, 1111, 1120, 1165, 1508
HpyF3I CTNAG 6 cut(s) 132, 960, 1081, 1405, 1411, 1670
HpySE526I ACGT 4 cut(s) 379, 789, 1008, 1421
Hsp92I GRCGYC 1 cut(s) 1421
Ksp22I TGATCA 2 cut(s) 382, 1186
KspI CCGCGG 1 cut(s) 1039
Kzo9I GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
LmnI GCTCC 5 cut(s) 169, 1096, 1374, 1472, 1703
Lsp1109I GCAGC 4 cut(s) 106, 193, 620, 1132
LweI GCATC 5 cut(s) 202, 613, 791, 1101, 1511
MaeII ACGT 4 cut(s) 379, 789, 1008, 1421
MaeIII GTNAC 3 cut(s) 230, 587, 857
MalI GATC 9 cut(s) 59, 334, 384, 875, 1048, 1188, 1274, 1334, 1567
MboI GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
MfeI CAATTG 1 cut(s) 999
MlsI TGGCCA 1 cut(s) 614
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 2 cut(s) 356, 1445
MmeI TCCRAC 6 cut(s) 772, 1022, 1116, 1118, 1404, 1604
MnlI CCTC 7 cut(s) 109, 710, 739, 1298, 1323, 1486, 1568
Mox20I TGGCCA 1 cut(s) 614
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 1076
MscI TGGCCA 1 cut(s) 614
MseI TTAA 4 cut(s) 258, 638, 1293, 1739
Msp20I TGGCCA 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 1038
MspCI CTTAAG 1 cut(s) 1292
MunI CAATTG 1 cut(s) 999
Mva1269I GAATGC 3 cut(s) 532, 668, 1178
MvnI CGCG 1 cut(s) 1038
MwoI GCNNNNNNNGC 7 cut(s) 128, 212, 941, 1111, 1120, 1165, 1508
NcoI CCATGG 2 cut(s) 609, 1328
NdeI CATATG 2 cut(s) 1222, 1348
NdeII GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
NlaIV GGNNCC 4 cut(s) 88, 171, 179, 1474
NmuCI GTSAC 2 cut(s) 587, 857
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 3 cut(s) 595, 761, 1746
PaeI GCATGC 1 cut(s) 761
PagI TCATGA 3 cut(s) 9, 385, 904
PaqCI CACCTGC 1 cut(s) 1388
PciI ACATGT 2 cut(s) 591, 1742
PctI GAATGC 3 cut(s) 532, 668, 1178
PdmI GAANNNNTTC 1 cut(s) 1076
PfeI GAWTC 2 cut(s) 920, 1633
PkrI GCNGC 5 cut(s) 121, 208, 635, 1037, 1122
Ple19I CGATCG 1 cut(s) 335
PleI GAGTC 2 cut(s) 356, 1444
PpsI GAGTC 2 cut(s) 356, 1444
PpuMI RGGWCCY 1 cut(s) 177
PscI ACATGT 2 cut(s) 591, 1742
Psp5II RGGWCCY 1 cut(s) 177
PspN4I GGNNCC 4 cut(s) 88, 171, 179, 1474
PspPI GGNCC 3 cut(s) 177, 779, 1201
PspPPI RGGWCCY 1 cut(s) 177
PstNI CAGNNNCTG 1 cut(s) 1114
PvuI CGATCG 1 cut(s) 335
RsaI GTAC 6 cut(s) 330, 453, 1055, 1367, 1515, 1655
RsaNI GTAC 6 cut(s) 329, 452, 1054, 1366, 1514, 1654
SacII CCGCGG 1 cut(s) 1039
SaqAI TTAA 4 cut(s) 258, 638, 1293, 1739
SatI GCNGC 5 cut(s) 120, 207, 634, 1036, 1121
Sau3AI GATC 9 cut(s) 57, 332, 382, 873, 1046, 1186, 1272, 1332, 1565
Sau96I GGNCC 3 cut(s) 177, 779, 1201
ScaI AGTACT 1 cut(s) 1515
SchI GAGTC 2 cut(s) 356, 1445
SfaNI GCATC 5 cut(s) 202, 613, 791, 1101, 1511
Sfr303I CCGCGG 1 cut(s) 1039
SgrBI CCGCGG 1 cut(s) 1039
SinI GGWCC 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 1292
SmoI CTYRAG 1 cut(s) 1292
SphI GCATGC 1 cut(s) 761
SsiI CCGC 3 cut(s) 84, 1036, 1038
SspI AATATT 1 cut(s) 553
StyI CCWWGG 4 cut(s) 173, 609, 843, 1328
TaaI ACNGT 8 cut(s) 19, 164, 495, 587, 941, 1270, 1488, 1495
TaiI ACGT 4 cut(s) 382, 792, 1011, 1424
TaqI TCGA 4 cut(s) 291, 335, 435, 1715
TatI WGTACW 4 cut(s) 451, 1053, 1365, 1513
TauI GCSGC 1 cut(s) 1038
TfiI GAWTC 2 cut(s) 920, 1633
Tru1I TTAA 4 cut(s) 258, 638, 1293, 1739
Tru9I TTAA 4 cut(s) 258, 638, 1293, 1739
TscAI CASTG 6 cut(s) 143, 169, 964, 1275, 1375, 1491
TseFI GTSAC 2 cut(s) 587, 857
TseI GCWGC 4 cut(s) 119, 206, 633, 1120
Tsp45I GTSAC 2 cut(s) 587, 857
TspRI CASTG 6 cut(s) 143, 169, 964, 1275, 1375, 1491
Vha464I CTTAAG 1 cut(s) 1292
VpaK11BI GGWCC 1 cut(s) 177
XagI CCTNNNNNAGG 1 cut(s) 1292
XapI RAATTY 5 cut(s) 125, 688, 1466, 1519, 1611
XceI RCATGY 3 cut(s) 595, 761, 1746
XmiI GTMKAC 1 cut(s) 582
XmnI GAANNNNTTC 1 cut(s) 1076
ZraI GACGTC 1 cut(s) 1422
ZrmI AGTACT 1 cut(s) 1515
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.