RLG00000036635

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000273
Physical Location & Seq
Forward (+)
82786950 .. 82791992
5043 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036635

Sequence Viewer

Length: 2175 bp
ATGCCAAGTGATGCAACATCGGAGGTGGACTTGGGCTTGGATTTAGGTATATGCTTGGTTTGGGTTTATAGGGCGAGTCACATACCAAAACCAGGAGAAGATGGAAGGTTTGGGTGGATGGGATTATTGGGCGCTGAGTTATGGTTCGGGCTTTATTGGATCCTCACCCAATCCTGCCGATGGAACCTTACCTTTAGGCAAACTTTCAAGGATAGACTATCTCAGAGATTTGAGAATGAGTTGCCGGGAGTGGACATTTTTGTGACCACGGCAGACCCTATCATAGAGCCACCAATGATGGTCATGAACACCGTTCTGTCAGTCATGGCTTATGATTACCCAACCGAGAAGATCAGCGTGTATCTATCCGATGATGGCGGTTCCGAGCTTACATACTACTCACTCTTGGAGGCTGCTGAGTTTGCCAAGCAGTGGATACCATACTGCAAGAAATACAGTGTGGAGCCAAGGTCCCCAGTTGCTTACTTTGTCTCAATAGCTACTGATGCAGTTGGTGTTGATGATAACAAGGCTAATGACTTGGCTGTCATTAAGAAATCATACAAGGACATGGAGACTAAAATCGAAAATGCAGTCAAATTAGGCCACATTCCAGAGAAAGTACGATCGAAACAGAAAGGCTTTTCTCAATGGGACTCACATTTGTCTAAACGTGATCATGACACCTATCTTAAAGTAGTAATTGATGGAAGAGACCCAAAGGCAATCGATGTTGAAGGACTTGTACTGCCAACTTTAGTGTATTTAGCTCGGGAGAAAAGACCACAGTATCACCATAACTTCAAAGCTGGTTCTATGAATGCATTGATTAGGGTTTCTTCAAATATTAGCAACGGGAAGGTCATTCTAAATGTAGACTGTGACATGTATTCAAACAATTCCATGGCCATAAGGGATGCACTTTGCTGCTTAATGGATGAAGAAAAAGGCCATGAAGTTGCATTCATACAATTCCCACAAAATTTTGAAAACCTCACCAAGAATGAATTATATATTCCATTCATGCGAACAATCAGCGAGGTGGAATTGCATGCTTTGGATGGTTTTGATGGCCCTTTTTACGTTGGAAGTGGATGCTTCCACAGAAGGGATACTCTTTGTGGAAGGAAGTTCACCAAGGGATGTAAGAGTGACATGAAATGGGAGATCAGTAGAAAAAGAGAAGAAACTACAATTCATGAATTGGAAGAAGATTCAAAAGTTCTTGCAAACTGTGCATACGAGCAAAACACTGAGTGGGGAAAAGAGATGGGTTTGAAATATGGTTGTGCAATTGAAGACGTGTTCACGGGGTTATCAATCCAATGCCGCGGTTGGAGATCGGTGTACTTGAACCCAACAAGGAAAGCTTTCTTAGGAGTAGCTCCAACGTCATTGCTTCAGATGCTGGTGCAGCAAAAGAGATGGTCGGAAGGTAGTTTCCAGATTTTGCTTTCACGGCACAATCCTGCATTCTCTGATCAGATTAGCTTGGGCCAAAAACTTGGATACGCATATGTCGGGGCTTGGTCTATCAATTTCCTACCAACCCTATTTTACTCAGTGATCCCTTCCCTTTACCTCCTTAAGGGCATTTCCTTATTTCCTCGGGTTTCAAGTCCATGGATCATACCATTTGCATATGTGATAATTGCCAAGTACACTGGGAGCTTTTTGGAGTTTCTTTGGGCAGGTGGCTCAGCCTTAGGTTGGTGGAACGACCAACGAATGTGGCTATACAAAAGATCAAGCTCCTACATATTTTCCTTCATCGACACCATTTTGTACCTTCTGGGATATTCTGATTCGGCATTTGTAATAACAGCCAAGACGTCGGACGAAGATGAGTCGCAACGATATGAGAAAGAGATTATGGAATTCGGAGTCTCTTCTCCACTGTTTACCGTTTTGGCATCACTTGCAGTACTAAATTTGTATTGCTTTGCTGGTTTTGTGAAGGAAGCAATCAGTCTGGGATCAAAGGGCGGCATTGGAGAAGTTTACGATACAATGGCATTGCAAATTCTACTATGTGGGATTTTGATTCTCATCAACCTGCCTTTGTACCAAGCCCTTTATCTAAGGAAGGACAAGGGGAAGTTGCCAAGCTCCATAGCTCTGAAGTCGATGGCATTTGGTGTCTTTGGTTTAACATGTTTCAATTTTTCGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

725

Amino Acids

82.01

Weight (kDa)

6.13

Isoelectric Point (pI)

39.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulose_synt PF03552 84 - 380 1.2e-89 Cellulose synthase
Cellulose_synt PF03552 397 - 654 3.1e-44 Cellulose synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55850
fragaria_vesca FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42770 FvH4_3g42800 FvH4_3g42810 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42900
malus_domestica MD03G1028800.v1.1 MD03G1028900.v1.1 MD03G1029000.v1.1 MD03G1029100.v1.1
prunus_persica Prupe.6G024800_v2.0.a1 Prupe.6G024900_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025100_v2.0.a1 Prupe.6G025100_v2.0.a1
pyrus_communis pycom01g03430 pycom03g02330 pycom03g02340 pycom03g02360 pycom03g02380 pycom03g02400 pycom03g02410 pycom05g13920
rosa_chinensis RchiOBHm_Chr5g0076821 RchiOBHm_Chr5g0076831 RchiOBHm_Chr5g0076851 RchiOBHm_Chr5g0076871 RchiOBHm_Chr5g0076891 RchiOBHm_Chr5g0076901 RchiOBHm_Chr5g0076911 RchiOBHm_Chr5g0076921
rosa_laevigata RLG00000036625 RLG00000036626 RLG00000036627 RLG00000036630 RLG00000036632 RLG00000036634 RLG00000036635
rosa_multiflora Rmu_co8084432.1_g000001 Rmu_co8208886.1_g000001 Rmu_co8325177.1_g000001 Rmu_sc0003799.1_g000001 Rmu_sc0003799.1_g000002 Rmu_sc0003799.1_g000006 Rmu_sc0003799.1_g000009 Rmu_sc0004655.1_g000002 Rmu_sc0004655.1_g000014 Rmu_sc0004655.1_g000015 Rmu_sc0007021.1_g000003 Rmu_sc0007021.1_g000013 Rmu_sc0007021.1_g000014 Rmu_sc0008244.1_g000002 Rmu_sc0013263.1_g000004 Rmu_sc0014402.1_g000001 Rmu_ssc0000184.1_g000003 Rmu_ssc0000184.1_g000004 Rmu_ssc0000184.1_g000005 Rmu_ssc0000184.1_g000006 Rmu_ssc0000184.1_g000014 Rmu_ssc0000184.1_g000021
rosa_roxburghii Rroxscaffold_1G00005210 Rroxscaffold_1G00005220 Rroxscaffold_1G00005250 Rroxscaffold_1G00005280 Rroxscaffold_1G00005300 Rroxscaffold_1G00005310
rosa_rugosa Rorug05G0449200 Rorug05G0449300 Rorug05G0449300 Rorug05G0449300 Rorug05G0449500 Rorug05G0449600
rosa_samantha Rh5AG503600 Rh5AG503700 Rh5AG503900 Rh5AG504000 Rh5AG504100 Rh5BG525000 Rh5BG525200 Rh5BG525300 Rh5BG525500 Rh5BG525700 Rh5BG525900 Rh5BG526000 Rh5CG548600 Rh5CG548800 Rh5CG548900 Rh5CG549100 Rh5CG549300 Rh5CG549500 Rh5DG537300 Rh5DG537400 Rh5DG537500 Rh5DG537700 Rh5DG537900 Rh5DG538000 Rh5DG544100
rosa_wichuraiana Rw0G009920 Rw5G046720 Rw5G046730 Rw5G047200 Rw5G047210 Rw5G047240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1682
AasI GACNNNNNNGTC 1 cut(s) 545
AatII GACGTC 1 cut(s) 1835
Acc36I ACCTGC 2 cut(s) 1682, 2064
AccB7I CCANNNNNTGG 1 cut(s) 432
AccI GTMKAC 1 cut(s) 876
AccII CGCG 1 cut(s) 1332
AciI CCGC 4 cut(s) 378, 1330, 1332, 1986
AclWI GGATC 5 cut(s) 154, 167, 1561, 1634, 1984
AcoI YGGCCR 1 cut(s) 906
AcsI RAATTY 4 cut(s) 982, 1877, 1930, 2022
AcuI CTGAAG 2 cut(s) 1385, 2141
AcyI GRCGYC 1 cut(s) 1832
AdeI CACNNNGTG 1 cut(s) 1257
AfaI GTAC 7 cut(s) 624, 747, 1349, 1661, 1787, 1926, 2066
AfiI CCNNNNNNNGG 6 cut(s) 92, 180, 432, 1108, 1588, 1710
AflII CTTAAG 1 cut(s) 1586
AflIII ACRYGT 3 cut(s) 885, 1302, 2153
AjiI CACGTC 1 cut(s) 1303
AjnI CCWGG 1 cut(s) 91
Alw26I GTCTC 4 cut(s) 496, 569, 708, 1891
AlwI GGATC 5 cut(s) 154, 167, 1561, 1634, 1984
AlwNI CAGNNNCTG 1 cut(s) 1408
Ama87I CYCGRG 2 cut(s) 771, 1608
AoxI GGCC 5 cut(s) 604, 906, 949, 1072, 1495
ApeKI GCWGC 3 cut(s) 413, 927, 1414
ApoI RAATTY 4 cut(s) 982, 1877, 1930, 2022
ArsI GACNNNNNNTTYG 2 cut(s) 579, 611
Asp700I GAANNNNTTC 1 cut(s) 1370
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 3 cut(s) 471, 1073, 1495
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 4 cut(s) 157, 785, 988, 1126
AvaI CYCGRG 2 cut(s) 771, 1608
AvaII GGWCC 1 cut(s) 471
AxyI CCTNAGG 1 cut(s) 1705
BaeI ACNNNNGTAYC 2 cut(s) 773, 806
BalI TGGCCA 1 cut(s) 908
BamHI GGATCC 1 cut(s) 159
BarI GAAGNNNNNNTAC 2 cut(s) 729, 761
BbsI GAAGAC 1 cut(s) 1305
BbvI GCAGC 3 cut(s) 400, 914, 1426
BceAI ACGGC 2 cut(s) 285, 1475
BciT130I CCWGG 1 cut(s) 93
BciVI GTATCC 3 cut(s) 429, 1105, 1502
BclI TGATCA 2 cut(s) 676, 1480
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 4 cut(s) 496, 569, 708, 1891
BfoI RGCGCY 1 cut(s) 135
BfrI CTTAAG 1 cut(s) 1586
BfuAI ACCTGC 2 cut(s) 1682, 2064
BfuI GTATCC 3 cut(s) 429, 1105, 1502
BisI GCNGC 5 cut(s) 414, 928, 1330, 1415, 1987
BlpI GCTNAGC 1 cut(s) 1699
BlsI GCNGC 5 cut(s) 415, 929, 1331, 1416, 1988
BmcAI AGTACT 1 cut(s) 1926
Bme1390I CCNGG 2 cut(s) 93, 246
Bme18I GGWCC 1 cut(s) 471
BmeT110I CYCGRG 2 cut(s) 771, 1608
BmgBI CACGTC 1 cut(s) 1303
BmgT120I GGNCC 3 cut(s) 471, 1073, 1495
BmiI GGNNCC 5 cut(s) 161, 185, 382, 465, 473
BmrFI CCNGG 2 cut(s) 93, 246
BmrI ACTGGG 2 cut(s) 470, 1674
BmsI GCATC 5 cut(s) 496, 907, 1085, 1395, 1922
BmuI ACTGGG 2 cut(s) 470, 1674
BpiI GAAGAC 1 cut(s) 1305
Bpu1102I GCTNAGC 1 cut(s) 1699
BpuMI CCSGG 1 cut(s) 246
Bsa29I ATCGAT 1 cut(s) 729
BsaBI GATNNNNATC 1 cut(s) 2048
BsaHI GRCGYC 1 cut(s) 1832
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 7 cut(s) 267, 467, 903, 1137, 1330, 1607, 1622
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 6 cut(s) 92, 180, 432, 1108, 1588, 1710
Bse1I ACTGG 2 cut(s) 476, 1669
Bse21I CCTNAGG 1 cut(s) 1705
Bse3DI GCAATG 2 cut(s) 1394, 2015
Bse8I GATNNNNATC 1 cut(s) 2048
BseBI CCWGG 1 cut(s) 93
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 7 cut(s) 267, 467, 903, 1137, 1330, 1607, 1622
BseGI GGATG 6 cut(s) 123, 922, 943, 1066, 1100, 1148
BseJI GATNNNNATC 1 cut(s) 2048
BseLI CCNNNNNNNGG 6 cut(s) 92, 180, 432, 1108, 1588, 1710
BseMI GCAATG 2 cut(s) 1394, 2015
BseMII CTCAG 6 cut(s) 126, 236, 408, 1245, 1575, 1713
BseNI ACTGG 2 cut(s) 476, 1669
BseXI GCAGC 3 cut(s) 400, 914, 1426
BsgI GTGCAG 1 cut(s) 1433
Bsh1236I CGCG 1 cut(s) 1332
Bsh1285I CGRYCG 1 cut(s) 629
BshFI GGCC 5 cut(s) 606, 908, 951, 1074, 1497
BshVI ATCGAT 1 cut(s) 729
BsiEI CGRYCG 1 cut(s) 629
BsiHKCI CYCGRG 2 cut(s) 771, 1608
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 2 cut(s) 457, 668
BslI CCNNNNNNNGG 6 cut(s) 92, 180, 432, 1108, 1588, 1710
BsmAI GTCTC 4 cut(s) 496, 569, 708, 1891
BsmFI GGGAC 2 cut(s) 457, 668
BsmI GAATGC 3 cut(s) 826, 962, 1472
BsnI GGCC 5 cut(s) 606, 908, 951, 1074, 1497
Bso31I GGTCTC 1 cut(s) 708
BsoBI CYCGRG 2 cut(s) 771, 1608
Bsp1720I GCTNAGC 1 cut(s) 1699
Bsp19I CCATGG 2 cut(s) 903, 1622
BspACI CCGC 4 cut(s) 378, 1330, 1332, 1986
BspANI GGCC 5 cut(s) 606, 908, 951, 1074, 1497
BspCNI CTCAG 6 cut(s) 127, 235, 409, 1246, 1574, 1712
BspDI ATCGAT 1 cut(s) 729
BspFNI CGCG 1 cut(s) 1332
BspHI TCATGA 3 cut(s) 303, 679, 1198
BspLI GGNNCC 5 cut(s) 161, 185, 382, 465, 473
BspMI ACCTGC 2 cut(s) 1682, 2064
BspPI GGATC 5 cut(s) 154, 167, 1561, 1634, 1984
BspTI CTTAAG 1 cut(s) 1586
BspTNI GGTCTC 1 cut(s) 708
BsrDI GCAATG 2 cut(s) 1394, 2015
BsrI ACTGG 2 cut(s) 476, 1669
BssECI CCNNGG 7 cut(s) 267, 467, 903, 1137, 1330, 1607, 1622
BssNI GRCGYC 1 cut(s) 1832
BssT1I CCWWGG 4 cut(s) 467, 903, 1137, 1622
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 7 cut(s) 313, 458, 789, 881, 1235, 1899, 1906
Bst6I CTCTTC 2 cut(s) 706, 1894
BstACI GRCGYC 1 cut(s) 1832
BstAFI CTTAAG 1 cut(s) 1586
BstAPI GCANNNNNTGC 2 cut(s) 1235, 1919
BstC8I GCNNGC 1 cut(s) 1053
BstDEI CTNAG 9 cut(s) 135, 222, 417, 1254, 1375, 1561, 1699, 1705, 2081
BstDSI CCRYGG 4 cut(s) 267, 903, 1330, 1622
BstENI CCTNNNNNAGG 1 cut(s) 1586
BstF5I GGATG 6 cut(s) 123, 922, 943, 1066, 1100, 1148
BstFNI CGCG 1 cut(s) 1332
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstMAI GTCTC 4 cut(s) 496, 569, 708, 1891
BstMCI CGRYCG 1 cut(s) 629
BstMWI GCNNNNNNNGC 7 cut(s) 422, 506, 1235, 1405, 1414, 1459, 1919
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 3 cut(s) 889, 1055, 2157
BstSCI CCNGG 2 cut(s) 91, 244
BstUI CGCG 1 cut(s) 1332
BstV1I GCAGC 3 cut(s) 400, 914, 1426
BstV2I GAAGAC 1 cut(s) 1305
BstX2I RGATCY 1 cut(s) 159
BstXI CCANNNNNNTGG 1 cut(s) 1505
BstYI RGATCY 1 cut(s) 159
Bsu15I ATCGAT 1 cut(s) 729
Bsu36I CCTNAGG 1 cut(s) 1705
BsuI GTATCC 3 cut(s) 429, 1105, 1502
BsuRI GGCC 5 cut(s) 606, 908, 951, 1074, 1497
BsuTUI ATCGAT 1 cut(s) 729
BtgI CCRYGG 4 cut(s) 267, 903, 1330, 1622
BtrI CACGTC 1 cut(s) 1303
BtsCI GGATG 6 cut(s) 123, 922, 943, 1066, 1100, 1148
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 6 cut(s) 437, 463, 1251, 1569, 1662, 1895
BveI ACCTGC 2 cut(s) 1682, 2064
Cac8I GCNNGC 1 cut(s) 1053
CaiI CAGNNNCTG 1 cut(s) 1408
CciI TCATGA 3 cut(s) 303, 679, 1198
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 3 cut(s) 471, 1073, 1495
Cfr42I CCGCGG 1 cut(s) 1333
ClaI ATCGAT 1 cut(s) 729
Csp6I GTAC 7 cut(s) 623, 746, 1348, 1660, 1786, 1925, 2065
CspCI CAANNNNNGTGG 2 cut(s) 1712, 1747
CviQI GTAC 7 cut(s) 623, 746, 1348, 1660, 1786, 1925, 2065
DdeI CTNAG 9 cut(s) 135, 222, 417, 1254, 1375, 1561, 1699, 1705, 2081
DraIII CACNNNGTG 1 cut(s) 1257
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 1 cut(s) 906
Eam1104I CTCTTC 2 cut(s) 706, 1894
EarI CTCTTC 2 cut(s) 706, 1894
Eco130I CCWWGG 4 cut(s) 467, 903, 1137, 1622
Eco31I GGTCTC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 471
Eco57I CTGAAG 2 cut(s) 1385, 2141
Eco81I CCTNAGG 1 cut(s) 1705
Eco88I CYCGRG 2 cut(s) 771, 1608
EcoNI CCTNNNNNAGG 1 cut(s) 1586
EcoO109I RGGNCCY 1 cut(s) 471
EcoRI GAATTC 1 cut(s) 1877
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 4 cut(s) 467, 903, 1137, 1622
EcoT22I ATGCAT 1 cut(s) 826
ErhI CCWWGG 4 cut(s) 467, 903, 1137, 1622
FalI AAGNNNNNCTT 2 cut(s) 1354, 1386
FaqI GGGAC 2 cut(s) 457, 668
FauNDI CATATG 2 cut(s) 1516, 1642
FbaI TGATCA 2 cut(s) 676, 1480
FblI GTMKAC 1 cut(s) 876
Fnu4HI GCNGC 5 cut(s) 414, 928, 1330, 1415, 1987
FokI GGATG 6 cut(s) 130, 929, 950, 1073, 1107, 1155
Fsp4HI GCNGC 5 cut(s) 414, 928, 1330, 1415, 1987
GlaI GCGC 1 cut(s) 133
GluI GCNGC 5 cut(s) 414, 928, 1330, 1415, 1987
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 5 cut(s) 606, 908, 951, 1074, 1497
HapII CCGG 1 cut(s) 245
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 1832
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 1368
HinfI GANTC 7 cut(s) 76, 656, 1214, 1805, 1847, 1884, 2044
HpaII CCGG 1 cut(s) 245
HphI GGTGA 4 cut(s) 157, 785, 988, 1126
Hpy166II GTNNAC 9 cut(s) 28, 253, 877, 1134, 1308, 1348, 1662, 1902, 2002
Hpy188III TCNNGA 6 cut(s) 304, 614, 680, 773, 1199, 1444
Hpy8I GTNNAC 9 cut(s) 28, 253, 877, 1134, 1308, 1348, 1662, 1902, 2002
Hpy99I CGWCG 1 cut(s) 1837
HpyCH4III ACNGT 7 cut(s) 313, 458, 789, 881, 1235, 1899, 1906
HpyCH4IV ACGT 5 cut(s) 673, 1083, 1302, 1391, 1832
HpyF10VI GCNNNNNNNGC 7 cut(s) 422, 506, 1235, 1405, 1414, 1459, 1919
HpyF3I CTNAG 9 cut(s) 135, 222, 417, 1254, 1375, 1561, 1699, 1705, 2081
HpySE526I ACGT 5 cut(s) 673, 1083, 1302, 1391, 1832
Hsp92I GRCGYC 1 cut(s) 1832
HspAI GCGC 1 cut(s) 132
Ksp22I TGATCA 2 cut(s) 676, 1480
KspI CCGCGG 1 cut(s) 1333
LmnI GCTCC 5 cut(s) 463, 1390, 1668, 1757, 2114
Lsp1109I GCAGC 3 cut(s) 400, 914, 1426
LweI GCATC 5 cut(s) 496, 907, 1085, 1395, 1922
MaeII ACGT 5 cut(s) 673, 1083, 1302, 1391, 1832
MaeIII GTNAC 4 cut(s) 77, 262, 881, 1151
MfeI CAATTG 1 cut(s) 1293
MflI RGATCY 1 cut(s) 159
MlsI TGGCCA 1 cut(s) 908
MluNI TGGCCA 1 cut(s) 908
MlyI GAGTC 4 cut(s) 85, 650, 1856, 1893
MmeI TCCRAC 5 cut(s) 1066, 1316, 1410, 1412, 1815
MnlI CCTC 7 cut(s) 16, 173, 403, 1004, 1033, 1592, 1617
Mox20I TGGCCA 1 cut(s) 908
Mph1103I ATGCAT 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 1370
MscI TGGCCA 1 cut(s) 908
MseI TTAA 5 cut(s) 552, 693, 932, 1587, 2150
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 908
MspA1I CMGCKG 1 cut(s) 1332
MspCI CTTAAG 1 cut(s) 1586
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 2 cut(s) 93, 246
MunI CAATTG 1 cut(s) 1293
Mva1269I GAATGC 3 cut(s) 826, 962, 1472
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 1 cut(s) 1332
MwoI GCNNNNNNNGC 7 cut(s) 422, 506, 1235, 1405, 1414, 1459, 1919
NciI CCSGG 1 cut(s) 246
NcoI CCATGG 2 cut(s) 903, 1622
NdeI CATATG 2 cut(s) 1516, 1642
NlaIV GGNNCC 5 cut(s) 161, 185, 382, 465, 473
NmuCI GTSAC 4 cut(s) 77, 262, 881, 1151
NsiI ATGCAT 1 cut(s) 826
NspI RCATGY 3 cut(s) 889, 1055, 2157
PaeI GCATGC 1 cut(s) 1055
PagI TCATGA 3 cut(s) 303, 679, 1198
PaqCI CACCTGC 1 cut(s) 1682
PciI ACATGT 2 cut(s) 885, 2153
PctI GAATGC 3 cut(s) 826, 962, 1472
PdmI GAANNNNTTC 1 cut(s) 1370
PfeI GAWTC 3 cut(s) 1214, 1805, 2044
PflMI CCANNNNNTGG 1 cut(s) 432
PkrI GCNGC 5 cut(s) 415, 929, 1331, 1416, 1988
Ple19I CGATCG 1 cut(s) 629
PleI GAGTC 4 cut(s) 84, 650, 1855, 1892
PpsI GAGTC 4 cut(s) 84, 650, 1855, 1892
PpuMI RGGWCCY 1 cut(s) 471
PscI ACATGT 2 cut(s) 885, 2153
Psp5II RGGWCCY 1 cut(s) 471
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 5 cut(s) 161, 185, 382, 465, 473
PspPI GGNCC 3 cut(s) 471, 1073, 1495
PspPPI RGGWCCY 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 1408
PsuI RGATCY 1 cut(s) 159
PvuI CGATCG 1 cut(s) 629
RsaI GTAC 7 cut(s) 624, 747, 1349, 1661, 1787, 1926, 2066
RsaNI GTAC 7 cut(s) 623, 746, 1348, 1660, 1786, 1925, 2065
RseI CAYNNNNRTG 1 cut(s) 260
SacII CCGCGG 1 cut(s) 1333
SaqAI TTAA 5 cut(s) 552, 693, 932, 1587, 2150
SatI GCNGC 5 cut(s) 414, 928, 1330, 1415, 1987
Sau96I GGNCC 3 cut(s) 471, 1073, 1495
ScaI AGTACT 1 cut(s) 1926
SchI GAGTC 4 cut(s) 85, 650, 1856, 1893
ScrFI CCNGG 2 cut(s) 93, 246
SfaNI GCATC 5 cut(s) 496, 907, 1085, 1395, 1922
Sfr303I CCGCGG 1 cut(s) 1333
SgrBI CCGCGG 1 cut(s) 1333
SinI GGWCC 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 1586
SmoI CTYRAG 1 cut(s) 1586
SphI GCATGC 1 cut(s) 1055
SsiI CCGC 4 cut(s) 378, 1330, 1332, 1986
SspI AATATT 1 cut(s) 847
StyD4I CCNGG 2 cut(s) 91, 244
StyI CCWWGG 4 cut(s) 467, 903, 1137, 1622
TaaI ACNGT 7 cut(s) 313, 458, 789, 881, 1235, 1899, 1906
TaiI ACGT 5 cut(s) 676, 1086, 1305, 1394, 1835
TaqI TCGA 5 cut(s) 585, 629, 729, 1773, 2126
TatI WGTACW 4 cut(s) 745, 1347, 1659, 1924
TauI GCSGC 2 cut(s) 1332, 1989
TfiI GAWTC 3 cut(s) 1214, 1805, 2044
Tru1I TTAA 5 cut(s) 552, 693, 932, 1587, 2150
Tru9I TTAA 5 cut(s) 552, 693, 932, 1587, 2150
TscAI CASTG 6 cut(s) 437, 463, 1258, 1569, 1669, 1902
TseFI GTSAC 4 cut(s) 77, 262, 881, 1151
TseI GCWGC 3 cut(s) 413, 927, 1414
Tsp45I GTSAC 4 cut(s) 77, 262, 881, 1151
TspRI CASTG 6 cut(s) 437, 463, 1258, 1569, 1669, 1902
Van91I CCANNNNNTGG 1 cut(s) 432
Vha464I CTTAAG 1 cut(s) 1586
VpaK11BI GGWCC 1 cut(s) 471
XagI CCTNNNNNAGG 1 cut(s) 1586
XapI RAATTY 4 cut(s) 982, 1877, 1930, 2022
XceI RCATGY 3 cut(s) 889, 1055, 2157
XmiI GTMKAC 1 cut(s) 876
XmnI GAANNNNTTC 1 cut(s) 1370
ZraI GACGTC 1 cut(s) 1833
ZrmI AGTACT 1 cut(s) 1926
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.