FvH4_6g39601

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000444
Physical Location & Seq
Reverse (-)
31279886 .. 31283423
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39601.t2

Sequence Viewer

Length: 2610 bp
ATGTCATCTTTGGAGGGTTTTGAGCCCGTAGCTATTTCCCCACACAAACAAGACCCAGGATGGAAGCACTGTCAAATTTTTAGTAAAATTGGGGACCCAAAAGTTGAGGTGAAGAAGTGTTTATATTGTGGCAAAGTGTTCCAGGGTGGAGGGATTAGTAGGCTTAAATTTCACCTTGCTGGTAGAAAAGGAAATGGCCCTATTTGTGATCAGGTCCCTCCCGATGTTCGGGTTTCGATGCTGCAGAACTTGGATGAAAAAGTTGGAACTTCTAGGCAGAGAAAGAGTCAATTGGGTACGAACCTGAGTCATAGTTTTAGTGAATTAGGGAATGTGTTTGCTGAAAATGAGGATAGCAGATTGGTTGGAGGGTCCGATTTGGTGAGTCAGGAGGAAGATGTGGGAATGAGGGTTGATAATGGGATTAGGGTTGGTGATAATTTGACTGATGTGCCTCTTGAGGTTGATAATTCTTTTAGGAGTGATGTGTCTCTTGCAATAGTCGACTCGTATAAGGGTAATTCTCTAAGGAGAAAAGGTGGAGTAGCGAATTCATGTGAAAATCGAGAGGAGGGTGTTGATAGACGAGTAGGGTGGAGGGGTGGAACTTCTTTTGTAGCTACTAGTGCCGGTTTGGGTTCTGGTTCTGTTAATGGTACCAAATTGAGTTATGTCCCAGCTCCTATTCGTAGTGCGGTACAAAGTCCATTTTACAGAAACACCAAAGGCAGATCAGTTGGTTATTCTGATTCAGATTTGTTTGAAAATGAAGCAGAAGTTGGCACAAGTAATACAAATGACAGGAAGAGGGTTAGAGATGAAAATGGATCAATGGCTGCCAATGCTGGCACTGTTGGCAACAATGGTGAAGTTGAAAAGGCGAACAGTCAGCAGATTCAGATGGCAATAGGACGATTTCTATATGAAATTCAGGCTCCTCTGGATGCTGTGAAGAACTCTCTTTATTTTCAGCCAATGATTGATGCAATTGCCTCTGGTGGAATGGAGAGTAAAGCACCCTCATATCATGATCTTCGGGGTTGGATACTGAATGATGCGGCTGAAGAAGTTAAGAATGAGATTTATCAGCACACTAATTCTTGGGAAAGGAATGGTTGCTCTCTTCTAGTAAACCAATTTAACTCCGAAAAGGGTAGGATATTGCTAAATTTCTCTGTGTACTGTCCTGAAGGAACTACATACTTGAAATCTGTGGATGCTTCTACATTTATAAATTCTCCAGATGCTTTGTATGAGATTCTTAAGCAAGTAGTGGAAGAAGTTGGAGTTAGGCGTGTTCTGCAAGTGATTACAAATAGTGAAGAGCATTATGTTGTTGCTGGGAAGAGGTTGATGGATACATTCCCAACGCTGTACTGGTCTCCCTGTGCTGCTGCGTGCATAAATTCGATACTTGAGGATTTTGGAAAATTTGAGTGGATAAACTCTATAATAGCACAGGCTAGATCTGTTACAAGATTTATCTATAAACACGTCGTCATTTTGAATATGATGCGGAGGTATACTTTTGGAAATGACATTGTGAAGCTGGGAATAACTCGGTATGCTACAGACTTTATGACTTTGAAACAAATGGCTGACCTTAAATTTAATTTGCAGACTATGGTTACTTCAAAAGAATGGGAAGGCTGCCCCTATTCTAAAACACCAGAAGGATTAGCAATGCTGGATTTATTAAGTAATCATACATTTTGGTCTTCGTGCATTATGATCACCCGATTTACAAATCCACTACTGCAAGTTTTGAGAATTGTTGGTAGCCAGAAGAAGGCAGCAATGGGATATGTTTTTGGTGGAATGTATCGAGCAAAAGAAACAATCAAGAGAGAACTTGTCAAGAAAGAAGTATATACAGCTTACTGGAATATCATAGATTACCGGTGGGCAAAATTATGGGATCACCCTCTTCATGCTGCTGGCTTTTACCTTAATCCTAAATTTTTCTATAGCATCAAGGGAGAAATGCACAAGGTGATTATGTCAAGGATGTTTGACTGCATAGAGAAATTGGTTCCAGATTTGAAAGTTCAAGACGAAATTAGCAAAGAGATAAACTTGTACCAAAACGCTGTTGGAGATATGGGGAGGAATTTGGCAATTAGAGCTAGAGACACTCTGCTTCCTGCTGAATGGTGGTCTACATATGGCAGTGGTTGCCCAAATATGGCGCGCTTGGCAGTTCATATTCTCAGTCAAACTTGCAGCTTAATTCAATGCAAGGAGAATCAGATTCCTTTTGACCAGTTGCACAAGACAAGGAACAGTTTAGAGCATCAACGTCTCAGTGATTTTGTATTTCTTCAGTACAACCTACAACTAAGGCAGATGGTTCATAAGAACAAAGAGCATGCATATGTGGATCCTATATCATTTGAAAACACTGGCGTTGTGGAAGACTGGGTAACGGAGCCTGAAATGTACCTAGAAAACGATGAAAATACTGATTGGAAGGCACTTGATCCACCCTCTTATAATTCAAGGCTTTTGGAACTTTCTGTTGACGAAGGTGAAGACTTGGGTTCAGGATTTGATGATTACGAGATTTTCTTTAATGGACTGCAAGTGGTTAAAGAAGAAGGCAGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

870

Amino Acids

97.94

Weight (kDa)

5.96

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 340 - 491 1.3e-55 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 699 - 778 1.6e-07 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1232, 2493
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccI GTMKAC 3 cut(s) 504, 1523, 2159
AccII CGCG 1 cut(s) 2191
AciI CCGC 3 cut(s) 695, 1058, 1516
AclWI GGATC 5 cut(s) 835, 1926, 2375, 2388, 2474
AcuI CTGAAG 3 cut(s) 1083, 1209, 2306
AdeI CACNNNGTG 1 cut(s) 1993
AfaI GTAC 8 cut(s) 298, 658, 699, 1181, 1376, 2081, 2327, 2441
AfiI CCNNNNNNNGG 3 cut(s) 228, 1789, 2185
AflII CTTAAG 1 cut(s) 1262
AflIII ACRYGT 1 cut(s) 1492
AgeI ACCGGT 1 cut(s) 1899
AhlI ACTAGT 1 cut(s) 623
AjiI CACGTC 1 cut(s) 1495
AjnI CCWGG 2 cut(s) 55, 141
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AluBI AGCT 7 cut(s) 32, 620, 680, 1549, 1877, 2126, 2226
AluI AGCT 7 cut(s) 32, 620, 680, 1549, 1877, 2126, 2226
Alw26I GTCTC 4 cut(s) 495, 1386, 2124, 2306
AlwI GGATC 5 cut(s) 835, 1926, 2375, 2388, 2474
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 8 cut(s) 241, 836, 1391, 1394, 1650, 1793, 1934, 2223
AsiGI ACCGGT 1 cut(s) 1899
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 2 cut(s) 2191, 2193
AspS9I GGNCC 4 cut(s) 94, 197, 214, 372
AsuHPI GGTGA 9 cut(s) 121, 164, 394, 446, 878, 1726, 1913, 2005, 2540
AvaII GGWCC 3 cut(s) 94, 214, 372
BamHI GGATCC 1 cut(s) 2380
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 3 cut(s) 1710, 2421, 2538
BbvI GCAGC 8 cut(s) 228, 823, 1378, 1381, 1637, 1805, 1921, 2235
BccI CCATC 4 cut(s) 54, 895, 1348, 2341
BciT130I CCWGG 2 cut(s) 57, 143
BciVI GTATCC 2 cut(s) 1038, 1351
BclI TGATCA 2 cut(s) 208, 1731
BcoDI GTCTC 4 cut(s) 495, 1386, 2124, 2306
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 6 cut(s) 273, 624, 1127, 1464, 2127, 2444
BfmI CTRYAG 3 cut(s) 242, 1569, 1966
BfrI CTTAAG 1 cut(s) 1262
BfuI GTATCC 2 cut(s) 1038, 1351
BglII AGATCT 1 cut(s) 1466
BisI GCNGC 9 cut(s) 242, 837, 1059, 1392, 1395, 1651, 1794, 1935, 2224
BlsI GCNGC 9 cut(s) 243, 838, 1060, 1393, 1396, 1652, 1795, 1936, 2225
Bme1390I CCNGG 2 cut(s) 57, 143
Bme18I GGWCC 3 cut(s) 94, 214, 372
BmgBI CACGTC 1 cut(s) 1495
BmgT120I GGNCC 4 cut(s) 94, 197, 214, 372
BmiI GGNNCC 9 cut(s) 95, 96, 216, 373, 658, 936, 2034, 2382, 2430
BmrFI CCNGG 2 cut(s) 57, 143
BmrI ACTGGG 1 cut(s) 2428
BmsI GCATC 9 cut(s) 228, 934, 973, 1045, 1207, 1234, 1503, 1980, 2302
BmuI ACTGGG 1 cut(s) 2428
BpiI GAAGAC 3 cut(s) 1710, 2421, 2538
BpmI CTGGAG 1 cut(s) 1224
BpuEI CTTGAG 2 cut(s) 479, 1436
BsaI GGTCTC 1 cut(s) 1386
BsaJI CCNNGG 2 cut(s) 55, 142
BsaWI WCCGGW 1 cut(s) 1899
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 1789, 2185
Bse118I RCCGGY 2 cut(s) 629, 1899
Bse1I ACTGG 5 cut(s) 1382, 1886, 2263, 2407, 2423
Bse3DI GCAATG 2 cut(s) 1689, 1803
BseBI CCWGG 2 cut(s) 57, 143
BseDI CCNNGG 2 cut(s) 55, 142
BseGI GGATG 5 cut(s) 65, 259, 949, 1222, 2013
BseLI CCNNNNNNNGG 3 cut(s) 228, 1789, 2185
BseMI GCAATG 2 cut(s) 1689, 1803
BseMII CTCAG 3 cut(s) 296, 2224, 2317
BseNI ACTGG 5 cut(s) 1382, 1886, 2263, 2407, 2423
BsePI GCGCGC 1 cut(s) 2189
BseRI GAGGAG 2 cut(s) 584, 927
BseXI GCAGC 8 cut(s) 228, 823, 1378, 1381, 1637, 1805, 1921, 2235
BseYI CCCAGC 3 cut(s) 676, 1340, 1549
Bsh1236I CGCG 1 cut(s) 2191
BshFI GGCC 1 cut(s) 198
BshNI GGYRCC 1 cut(s) 656
BshTI ACCGGT 1 cut(s) 1899
BsiSI CCGG 2 cut(s) 630, 1900
BslFI GGGAC 3 cut(s) 107, 200, 659
BslI CCNNNNNNNGG 3 cut(s) 228, 1789, 2185
BsmAI GTCTC 4 cut(s) 495, 1386, 2124, 2306
BsmBI CGTCTC 1 cut(s) 2306
BsmFI GGGAC 3 cut(s) 107, 200, 659
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 1386
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
BspACI CCGC 3 cut(s) 695, 1058, 1516
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 3 cut(s) 297, 2223, 2316
BspFNI CGCG 1 cut(s) 2191
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 9 cut(s) 95, 96, 216, 373, 658, 936, 2034, 2382, 2430
BspMAI CTGCAG 1 cut(s) 246
BspPI GGATC 5 cut(s) 835, 1926, 2375, 2388, 2474
BspQI GCTCTTC 1 cut(s) 1317
BspT107I GGYRCC 1 cut(s) 656
BspTI CTTAAG 1 cut(s) 1262
BspTNI GGTCTC 1 cut(s) 1386
BsrDI GCAATG 2 cut(s) 1689, 1803
BsrFI RCCGGY 2 cut(s) 629, 1899
BsrI ACTGG 5 cut(s) 1382, 1886, 2263, 2407, 2423
BssAI RCCGGY 2 cut(s) 629, 1899
BssECI CCNNGG 2 cut(s) 55, 142
BssHII GCGCGC 1 cut(s) 2189
BssMI GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
BssNAI GTATAC 1 cut(s) 1524
Bst1107I GTATAC 1 cut(s) 1524
Bst2UI CCWGG 2 cut(s) 57, 143
Bst4CI ACNGT 5 cut(s) 71, 853, 887, 1184, 2285
Bst6I CTCTTC 5 cut(s) 800, 1128, 1317, 1340, 1932
BstAFI CTTAAG 1 cut(s) 1262
BstAPI GCANNNNNTGC 1 cut(s) 2175
BstC8I GCNNGC 5 cut(s) 847, 1399, 1939, 2191, 2370
BstDEI CTNAG 5 cut(s) 305, 527, 2210, 2303, 2339
BstF5I GGATG 5 cut(s) 65, 259, 949, 1222, 2013
BstFNI CGCG 1 cut(s) 2191
BstHHI GCGC 2 cut(s) 2191, 2193
BstKTI GATC 9 cut(s) 211, 734, 830, 1033, 1469, 1734, 1921, 2383, 2482
BstMAI GTCTC 4 cut(s) 495, 1386, 2124, 2306
BstMBI GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
BstMWI GCNNNNNNNGC 7 cut(s) 626, 842, 855, 1300, 2123, 2175, 2195
BstNI CCWGG 2 cut(s) 57, 143
BstNSI RCATGY 1 cut(s) 2372
BstSCI CCNGG 2 cut(s) 55, 141
BstSFI CTRYAG 3 cut(s) 242, 1569, 1966
BstUI CGCG 1 cut(s) 2191
BstV1I GCAGC 8 cut(s) 228, 823, 1378, 1381, 1637, 1805, 1921, 2235
BstV2I GAAGAC 3 cut(s) 1710, 2421, 2538
BstX2I RGATCY 2 cut(s) 1466, 2380
BstYI RGATCY 2 cut(s) 1466, 2380
BstZ17I GTATAC 1 cut(s) 1524
BsuI GTATCC 2 cut(s) 1038, 1351
BsuRI GGCC 1 cut(s) 198
BtrI CACGTC 1 cut(s) 1495
BtsCI GGATG 5 cut(s) 65, 259, 949, 1222, 2013
BtsI GCAGTG 1 cut(s) 2176
BtsIMutI CAGTG 5 cut(s) 67, 849, 2176, 2311, 2400
Cac8I GCNNGC 5 cut(s) 847, 1399, 1939, 2191, 2370
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 2 cut(s) 2191, 2193
Cfr10I RCCGGY 2 cut(s) 629, 1899
Cfr13I GGNCC 4 cut(s) 94, 197, 214, 372
Csp6I GTAC 8 cut(s) 297, 657, 698, 1180, 1375, 2080, 2326, 2440
CspAI ACCGGT 1 cut(s) 1899
CviAII CATG 5 cut(s) 555, 1028, 1931, 2369, 2607
CviQI GTAC 8 cut(s) 297, 657, 698, 1180, 1375, 2080, 2326, 2440
DdeI CTNAG 5 cut(s) 305, 527, 2210, 2303, 2339
DpnI GATC 9 cut(s) 210, 733, 829, 1032, 1468, 1733, 1920, 2382, 2481
DpnII GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
DraIII CACNNNGTG 1 cut(s) 1993
Eam1104I CTCTTC 5 cut(s) 800, 1128, 1317, 1340, 1932
EarI CTCTTC 5 cut(s) 800, 1128, 1317, 1340, 1932
Eco24I GRGCYC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 1386
Eco47I GGWCC 3 cut(s) 94, 214, 372
Eco57I CTGAAG 3 cut(s) 1083, 1209, 2306
EcoO109I RGGNCCY 2 cut(s) 94, 214
EcoRI GAATTC 1 cut(s) 550
EcoRII CCWGG 2 cut(s) 55, 141
EcoT22I ATGCAT 1 cut(s) 2374
EcoT38I GRGCYC 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 2306
FaeI CATG 5 cut(s) 558, 1031, 1934, 2372, 2610
FaqI GGGAC 3 cut(s) 107, 200, 659
FatI CATG 5 cut(s) 554, 1027, 1930, 2368, 2606
FauNDI CATATG 2 cut(s) 2164, 2374
FbaI TGATCA 2 cut(s) 208, 1731
FblI GTMKAC 3 cut(s) 504, 1523, 2159
Fnu4HI GCNGC 9 cut(s) 242, 837, 1059, 1392, 1395, 1651, 1794, 1935, 2224
FokI GGATG 5 cut(s) 72, 266, 956, 1229, 2020
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 9 cut(s) 242, 837, 1059, 1392, 1395, 1651, 1794, 1935, 2224
FspBI CTAG 6 cut(s) 273, 624, 1127, 1464, 2127, 2444
GlaI GCGC 2 cut(s) 2190, 2192
GluI GCNGC 9 cut(s) 242, 837, 1059, 1392, 1395, 1651, 1794, 1935, 2224
GsaI CCCAGC 3 cut(s) 680, 1344, 1553
GsuI CTGGAG 1 cut(s) 1224
HaeIII GGCC 1 cut(s) 198
HapII CCGG 2 cut(s) 630, 1900
HhaI GCGC 2 cut(s) 2191, 2193
Hin1II CATG 5 cut(s) 558, 1031, 1934, 2372, 2610
Hin6I GCGC 2 cut(s) 2189, 2191
HinP1I GCGC 2 cut(s) 2189, 2191
HincII GTYRAC 2 cut(s) 505, 2521
HindII GTYRAC 2 cut(s) 505, 2521
HinfI GANTC 9 cut(s) 286, 307, 385, 506, 749, 895, 1258, 2245, 2251
HpaII CCGG 2 cut(s) 630, 1900
HphI GGTGA 9 cut(s) 121, 164, 394, 446, 878, 1726, 1913, 2005, 2540
Hpy166II GTNNAC 6 cut(s) 505, 1132, 1180, 1524, 2160, 2521
Hpy188I TCNGA 6 cut(s) 376, 748, 754, 900, 1147, 2250
Hpy8I GTNNAC 6 cut(s) 505, 1132, 1180, 1524, 2160, 2521
Hpy99I CGWCG 1 cut(s) 1499
HpyAV CCTTC 7 cut(s) 1184, 1640, 1667, 1783, 2464, 2519, 2591
HpyCH4III ACNGT 5 cut(s) 71, 853, 887, 1184, 2285
HpyCH4IV ACGT 2 cut(s) 1494, 2299
HpyF10VI GCNNNNNNNGC 7 cut(s) 626, 842, 855, 1300, 2123, 2175, 2195
HpyF3I CTNAG 5 cut(s) 305, 527, 2210, 2303, 2339
HpySE526I ACGT 2 cut(s) 1494, 2299
Hsp92II CATG 5 cut(s) 558, 1031, 1934, 2372, 2610
HspAI GCGC 2 cut(s) 2189, 2191
KflI GGGWCCC 1 cut(s) 94
KpnI GGTACC 1 cut(s) 660
Ksp22I TGATCA 2 cut(s) 208, 1731
Kzo9I GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
LguI GCTCTTC 1 cut(s) 1317
LmnI GCTCC 3 cut(s) 685, 940, 2428
Lsp1109I GCAGC 8 cut(s) 228, 823, 1378, 1381, 1637, 1805, 1921, 2235
LweI GCATC 9 cut(s) 228, 934, 973, 1045, 1207, 1234, 1503, 1980, 2302
MaeI CTAG 6 cut(s) 273, 624, 1127, 1464, 2127, 2444
MaeII ACGT 2 cut(s) 1494, 2299
MaeIII GTNAC 3 cut(s) 1471, 1627, 2422
MalI GATC 9 cut(s) 210, 733, 829, 1032, 1468, 1733, 1920, 2382, 2481
MboI GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
MfeI CAATTG 2 cut(s) 290, 987
MflI RGATCY 2 cut(s) 1466, 2380
MhlI GDGCHC 1 cut(s) 27
MlyI GAGTC 4 cut(s) 295, 316, 394, 500
MmeI TCCRAC 5 cut(s) 244, 346, 1022, 1264, 2074
Mph1103I ATGCAT 1 cut(s) 2374
MslI CAYNNNNRTG 1 cut(s) 2373
MspCI CTTAAG 1 cut(s) 1262
MspI CCGG 2 cut(s) 630, 1900
MspR9I CCNGG 2 cut(s) 57, 143
MunI CAATTG 2 cut(s) 290, 987
MvaI CCWGG 2 cut(s) 57, 143
MvnI CGCG 1 cut(s) 2191
MwoI GCNNNNNNNGC 7 cut(s) 626, 842, 855, 1300, 2123, 2175, 2195
NdeI CATATG 2 cut(s) 2164, 2374
NdeII GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
NlaIII CATG 5 cut(s) 558, 1031, 1934, 2372, 2610
NlaIV GGNNCC 9 cut(s) 95, 96, 216, 373, 658, 936, 2034, 2382, 2430
NsiI ATGCAT 1 cut(s) 2374
NspI RCATGY 1 cut(s) 2372
PaeI GCATGC 1 cut(s) 2372
PagI TCATGA 1 cut(s) 1027
PauI GCGCGC 1 cut(s) 2189
PciSI GCTCTTC 1 cut(s) 1317
PfeI GAWTC 5 cut(s) 749, 895, 1258, 2245, 2251
PinAI ACCGGT 1 cut(s) 1899
PkrI GCNGC 9 cut(s) 243, 838, 1060, 1393, 1396, 1652, 1795, 1936, 2225
PleI GAGTC 4 cut(s) 294, 315, 393, 500
PpsI GAGTC 4 cut(s) 294, 315, 393, 500
PpuMI RGGWCCY 2 cut(s) 94, 214
PsiI TTATAA 2 cut(s) 1232, 2493
Psp5II RGGWCCY 2 cut(s) 94, 214
Psp6I CCWGG 2 cut(s) 55, 141
PspFI CCCAGC 3 cut(s) 676, 1340, 1549
PspGI CCWGG 2 cut(s) 55, 141
PspN4I GGNNCC 9 cut(s) 95, 96, 216, 373, 658, 936, 2034, 2382, 2430
PspPI GGNCC 4 cut(s) 94, 197, 214, 372
PspPPI RGGWCCY 2 cut(s) 94, 214
PstI CTGCAG 1 cut(s) 246
PsuI RGATCY 2 cut(s) 1466, 2380
PteI GCGCGC 1 cut(s) 2189
RsaI GTAC 8 cut(s) 298, 658, 699, 1181, 1376, 2081, 2327, 2441
RsaNI GTAC 8 cut(s) 297, 657, 698, 1180, 1375, 2080, 2326, 2440
RseI CAYNNNNRTG 1 cut(s) 2373
SalI GTCGAC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 1317
SatI GCNGC 9 cut(s) 242, 837, 1059, 1392, 1395, 1651, 1794, 1935, 2224
Sau3AI GATC 9 cut(s) 208, 731, 827, 1030, 1466, 1731, 1918, 2380, 2479
Sau96I GGNCC 4 cut(s) 94, 197, 214, 372
SchI GAGTC 4 cut(s) 295, 316, 394, 500
ScrFI CCNGG 2 cut(s) 57, 143
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 9 cut(s) 228, 934, 973, 1045, 1207, 1234, 1503, 1980, 2302
SfcI CTRYAG 3 cut(s) 242, 1569, 1966
SinI GGWCC 3 cut(s) 94, 214, 372
SmiMI CAYNNNNRTG 1 cut(s) 2373
SmlI CTYRAG 3 cut(s) 458, 1262, 1415
SmoI CTYRAG 3 cut(s) 458, 1262, 1415
SpeI ACTAGT 1 cut(s) 623
SphI GCATGC 1 cut(s) 2372
SsiI CCGC 3 cut(s) 695, 1058, 1516
SspMI CTAG 6 cut(s) 273, 624, 1127, 1464, 2127, 2444
StyD4I CCNGG 2 cut(s) 55, 141
TaaI ACNGT 5 cut(s) 71, 853, 887, 1184, 2285
TaiI ACGT 2 cut(s) 1497, 2302
TaqI TCGA 5 cut(s) 236, 504, 565, 1409, 1825
TatI WGTACW 3 cut(s) 1179, 1374, 2325
TauI GCSGC 1 cut(s) 1061
TfiI GAWTC 5 cut(s) 749, 895, 1258, 2245, 2251
TscAI CASTG 5 cut(s) 74, 856, 2176, 2311, 2407
TseI GCWGC 8 cut(s) 241, 836, 1391, 1394, 1650, 1793, 1934, 2223
TspDTI ATGAA 9 cut(s) 270, 543, 783, 834, 939, 1919, 2192, 2342, 2469
TspGWI ACGGA 1 cut(s) 2441
TspRI CASTG 5 cut(s) 74, 856, 2176, 2311, 2407
Vha464I CTTAAG 1 cut(s) 1262
VpaK11BI GGWCC 3 cut(s) 94, 214, 372
XceI RCATGY 1 cut(s) 2372
XcmI CCANNNNNNNNNTGG 2 cut(s) 1374, 2090
XmiI GTMKAC 3 cut(s) 504, 1523, 2159
XspI CTAG 6 cut(s) 273, 624, 1127, 1464, 2127, 2444
Zsp2I ATGCAT 1 cut(s) 2374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.