Protein of unknown function (DUF 659)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 141 |
| AciI | CCGC | 1 cut(s) | 141 |
| AclWI | GGATC | 1 cut(s) | 44 |
| AcsI | RAATTY | 1 cut(s) | 132 |
| AgsI | TTSAA | 2 cut(s) | 101, 148 |
| AlwI | GGATC | 1 cut(s) | 44 |
| Ama87I | CYCGRG | 1 cut(s) | 67 |
| ApoI | RAATTY | 1 cut(s) | 132 |
| AvaI | CYCGRG | 1 cut(s) | 67 |
| BbsI | GAAGAC | 1 cut(s) | 24 |
| BmeT110I | CYCGRG | 1 cut(s) | 67 |
| BpiI | GAAGAC | 1 cut(s) | 24 |
| BseGI | GGATG | 1 cut(s) | 45 |
| BseMII | CTCAG | 1 cut(s) | 51 |
| BsiHKCI | CYCGRG | 1 cut(s) | 67 |
| BsoBI | CYCGRG | 1 cut(s) | 67 |
| Bsp143I | GATC | 1 cut(s) | 49 |
| BspACI | CCGC | 1 cut(s) | 141 |
| BspCNI | CTCAG | 1 cut(s) | 52 |
| BspPI | GGATC | 1 cut(s) | 44 |
| BsrBI | CCGCTC | 1 cut(s) | 141 |
| BssMI | GATC | 1 cut(s) | 49 |
| BstDEI | CTNAG | 1 cut(s) | 60 |
| BstF5I | GGATG | 1 cut(s) | 45 |
| BstKTI | GATC | 1 cut(s) | 52 |
| BstMBI | GATC | 1 cut(s) | 49 |
| BstV2I | GAAGAC | 1 cut(s) | 24 |
| BtsCI | GGATG | 1 cut(s) | 45 |
| BtsIMutI | CAGTG | 1 cut(s) | 21 |
| CviJI | RGCY | 1 cut(s) | 73 |
| CviKI_1 | RGCY | 1 cut(s) | 73 |
| DdeI | CTNAG | 1 cut(s) | 60 |
| DpnI | GATC | 1 cut(s) | 51 |
| DpnII | GATC | 1 cut(s) | 49 |
| Eco88I | CYCGRG | 1 cut(s) | 67 |
| FokI | GGATG | 1 cut(s) | 52 |
| Hpy188I | TCNGA | 1 cut(s) | 61 |
| Hpy188III | TCNNGA | 1 cut(s) | 32 |
| HpyCH4V | TGCA | 1 cut(s) | 10 |
| HpyF3I | CTNAG | 1 cut(s) | 60 |
| Kzo9I | GATC | 1 cut(s) | 49 |
| LpnPI | CCDG | 2 cut(s) | 45, 99 |
| MaeIII | GTNAC | 1 cut(s) | 42 |
| MalI | GATC | 1 cut(s) | 51 |
| MbiI | CCGCTC | 1 cut(s) | 141 |
| MboI | GATC | 1 cut(s) | 49 |
| MboII | GAAGA | 2 cut(s) | 29, 113 |
| MluCI | AATT | 2 cut(s) | 104, 132 |
| MnlI | CCTC | 2 cut(s) | 63, 67 |
| MseI | TTAA | 2 cut(s) | 156, 169 |
| NdeII | GATC | 1 cut(s) | 49 |
| NmuCI | GTSAC | 1 cut(s) | 42 |
| PaeR7I | CTCGAG | 1 cut(s) | 67 |
| PspXI | VCTCGAGB | 1 cut(s) | 67 |
| SaqAI | TTAA | 2 cut(s) | 156, 169 |
| Sau3AI | GATC | 1 cut(s) | 49 |
| Sfr274I | CTCGAG | 1 cut(s) | 67 |
| SgeI | CNNG | 6 cut(s) | 26, 44, 59, 79, 81, 126 |
| SlaI | CTCGAG | 1 cut(s) | 67 |
| SmlI | CTYRAG | 1 cut(s) | 67 |
| SmoI | CTYRAG | 1 cut(s) | 67 |
| Sse9I | AATT | 2 cut(s) | 104, 132 |
| SsiI | CCGC | 1 cut(s) | 141 |
| TaqI | TCGA | 1 cut(s) | 68 |
| TasI | AATT | 2 cut(s) | 104, 132 |
| Tru1I | TTAA | 2 cut(s) | 156, 169 |
| Tru9I | TTAA | 2 cut(s) | 156, 169 |
| TscAI | CASTG | 1 cut(s) | 28 |
| TseFI | GTSAC | 1 cut(s) | 42 |
| Tsp45I | GTSAC | 1 cut(s) | 42 |
| TspDTI | ATGAA | 2 cut(s) | 108, 144 |
| TspRI | CASTG | 1 cut(s) | 28 |
| XapI | RAATTY | 1 cut(s) | 132 |
| XhoI | CTCGAG | 1 cut(s) | 67 |