Prupe.2G058600_v2.0.a1

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000444
Physical Location & Seq
Reverse (-)
7305985 .. 7313979
7995 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G058600.1

Sequence Viewer

Length: 2733 bp
ATGACGTCGTTTCATTACAATGAACCTGAGGAAAAAAAAAAAAAAAAACACGCGAGTCGTCTTCCTCGCAGAGTCGCAGCCAATTTGCAGAGTCGCAAAATTTCTTCCGGTTCAGAAGTGTCCAGATCTGGCGCGCCTTGCTCCGCCTCACTCCGCCTGGCCACGCCTTGCAACGCCTCCTCACGCCTTCCCCGCGCTAAACGTGCAACTCGTGAAACAGCCGACCGCCTCGGGCGATTTCCTGCCGCCTCTCTCCGACGCGCTCGCCGACGCGCGCGAAGGCATGCCTTTGAAAACACTGCTCAGGAGCTTTCTGACACTTCCCCAGATATGCAGTACAAGGATACTCAAGTTTCAGTGCCTCCCCGTGCATCCGATCCTGGCTGGGCTCATGGTATTATGGTGAATGGAGGTCGCCAGAAGATTAAGTGCAAATACTGCCATAAAGTAATGCTTGGGGGCGGAATTTCCAGACTAAAACAGCATTTGGCTGGGGAAAGGGGGAATGTAGCTCCGTGTGAGGAAGTACCTGAAGAAGTTAAAGTGCAGATTCAACAACATCTTGGTTTTAAAGTACTGGAGAAGCTTAAAAAGCAGAAAGGATTAAGTAATAGCAAGGATTCCGCACCCTACTTGCAGTATAGGGAAGGAGGAGAAAATGAAGATGGAATGAGAACTCAAAAGACCGTTTGCACACGAGGCCAGCGAAGGAGAAGGGAAAAAGAGGCTATGGAATGCATTTCAAATCAAATGAAGAAGCACAAAAAACGTTCTTTCCCTGCTGCAACAACTATTGTCGCTCAGCCTTTACATCAGAGTTTTGCTTCTCAAGAGATCATAGCCCAAGCTGATTTGGCTGTTGCAAGGTTTATGTATGAATCCGGTATACCATTCACTGCTGCGAATTCGCATTTTTATCAACAGATGGCTGATGCCATTGCTGCTGCAGGACCTGGCTATAAGATGCCTTCTTATCATGCTTTGAGGGGAAAACTGTTGAACAAAAGCGTTCAGGATGCAGAAGAATATGAAGAGGAACTCAGAAAATCTTGGGATGTGACTGGGTGTTCTGTCATGGTTGATAGGTGGGTGGATAAAACTGGTAATTCAGTTATAAACTTCTTTGTATACTGTCCTAAAGGCACTCTGTTCCTCAAATCTGCAGATGCATCAGACATTTCAGAATGTCCAGATGCACTTCTTAATTTATTTGACTGCGTTGTTCAAGAAGTTGGGCATAAGAGGATTGTCAATTTTGTCACAGATGCTTCAGCCAGTTGTAAAGCTGCGAGAAAACTTCTAATGGAGAAATATAAAACCTTTTTCTGTAGTACGTGTGGTGGATATGGTATTGAATTAATGCTTGAGGAAATTGGAAAAATGAATGAAGTTAAAGAGGTCTTGGCAAAGGCAAAAAAGATTACTCAATTTATATACAACAGTGCTTGGATGCTTAATCTAGTAAGGAGGAAAACAGGTGGAAGAGACATAGTCCAGCTCGCAACCACAAGATTTGCTTCCACCTTCCTGACACTACAGAATATGGTAGCTTTGAAGCACCGGCTCGAAAAGATATTTGCCAGTGCTGCTTGGATGCATTCAACCTTCTCTAAGCAAAAGGCTGGACTTGAGCTGGCAGAGATTATTGCGGACCAACTTTTCTGGTCACTGTGTGATCAGATTCTGAAGGTGACTAAGCCCCTACTTTCTGTTTTTCAGCTTATGGACTGCGAGGAGAAGCCATCTATTGGCTATGTGTATGATGCAATGGAAAAAGCAAAGAAAAGTATAATTGTGTCATTTGACAATAAGGAATCTGACTACGTGCCATATCTGGAGATTATTGATCACATTTGGCAAGAGGAGCTTCATAGTCCCCTTCATGCAGCTGCATACTATCTCAACCCATCTATATTTTATAACCCTAGCTTTTCTACTAACAAAGTCATCCAGAAGGGATTGCTGGACTGTATTGAAACCTTAGAGCCTAACTTAACAGCTCAAGTTGTGATTACGAGCAATATAAATTTCTACGAGGAAGCTGTAGGGGATTTTGGTCGACCTGTCGCATTACGTTGTCGAGAATCATTGGCCCCAGCTACTTGGTGGTCACTGTATGCAGCAGATTATCCCGATCTCCAACGATTAGCTGTCAGGATACTAAGTCAAACTTGCAGCATTATTCGATATAAGCGGAGTAGGACCATGTTTGAACGTATGTGTTCGAAGAAAAAGAACCGATTGGAGCAACAAAGGTTCAACCACCTTGCATTTGTTCATTATAACTTGCACCTTCAACATAGGCGTTCTGAAGCAAGTAAAGCCATTTATACGAGAGGTACACTCGATCCTATATGCTTAGAAGCTATCGATGCCAACATGGGAGATTGGGTGGAAGATCTGGGAGCTATTGGCAGCGATGGCCTAAGCTGGATGGACGTGACAGTCCCTGAAGTTAGCATTTATTTTCAGCTTTTTGGTCATTGGTCTTCTATTGCCTATAGCATATATGTCCAAAGAAAGAAGGAAATTAACTTCCAGAATTTAGTGAAAAAGTTGATCGCCGTTGTGGATGATGGGAGAAGCTATAATGGTCGAAGAGAGGAGAGGACTACATCTTGGAGAGAGCTGTCTCTCTGCATGGCAGCTTTGAACTCATCAAAGTATAATGTGAATAGAAACGACTTACAGCCATTATCATCTGTTGCTTTTGCCGATTCGACTGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

911

Amino Acids

103.13

Weight (kDa)

9.25

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1115, 1920, 2283
AasI GACNNNNNNGTC 1 cut(s) 2444
AatII GACGTC 1 cut(s) 8
AccB7I CCANNNNNTGG 1 cut(s) 1748
AccI GTMKAC 3 cut(s) 886, 1128, 2059
AccII CGCG 7 cut(s) 53, 134, 195, 261, 273, 275, 277
AciI CCGC 9 cut(s) 144, 154, 193, 226, 246, 462, 624, 1649, 2194
AclI AACGTT 1 cut(s) 769
AclWI GGATC 2 cut(s) 371, 2342
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 5 cut(s) 99, 465, 904, 2026, 2542
AcuI CTGAAG 5 cut(s) 552, 1254, 1706, 2331, 2472
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1673
AfaI GTAC 5 cut(s) 338, 528, 576, 1333, 2341
AfiI CCNNNNNNNGG 1 cut(s) 1748
AflIII ACRYGT 1 cut(s) 1334
AjiI CACGTC 1 cut(s) 2440
AjnI CCWGG 3 cut(s) 156, 379, 952
Alw26I GTCTC 2 cut(s) 1479, 2637
AlwI GGATC 2 cut(s) 371, 2342
AlwNI CAGNNNCTG 3 cut(s) 953, 1684, 2450
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 4 cut(s) 159, 700, 2091, 2422
ApoI RAATTY 5 cut(s) 99, 465, 904, 2026, 2542
AscI GGCGCGCC 1 cut(s) 132
AseI ATTAAT 1 cut(s) 1358
AspLEI GCGC 6 cut(s) 134, 136, 197, 263, 275, 277
AspS9I GGNCC 4 cut(s) 950, 1651, 2092, 2202
AsuHPI GGTGA 2 cut(s) 415, 1702
AsuII TTCGAA 1 cut(s) 2225
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 3 cut(s) 950, 1651, 2202
AxyI CCTNAGG 1 cut(s) 27
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BalI TGGCCA 1 cut(s) 161
BanII GRGCYC 1 cut(s) 391
BauI CACGAG 2 cut(s) 210, 696
BbsI GAAGAC 2 cut(s) 53, 2481
BccI CCATC 7 cut(s) 659, 919, 1750, 1915, 2414, 2428, 2570
BceAI ACGGC 1 cut(s) 2549
BciT130I CCWGG 3 cut(s) 158, 381, 954
BciVI GTATCC 2 cut(s) 337, 2151
BclI TGATCA 2 cut(s) 1675, 1846
BcoDI GTCTC 2 cut(s) 1479, 2637
BfaI CTAG 2 cut(s) 1460, 1926
BfmI CTRYAG 6 cut(s) 945, 1161, 1327, 1535, 2043, 2500
BfuI GTATCC 2 cut(s) 337, 2151
BglII AGATCT 2 cut(s) 125, 2398
BlpI GCTNAGC 1 cut(s) 801
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 3 cut(s) 158, 381, 954
Bme18I GGWCC 3 cut(s) 950, 1651, 2202
BmeT110I CYCGRG 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 2440
BmgT120I GGNCC 4 cut(s) 950, 1651, 2092, 2202
BmiI GGNNCC 1 cut(s) 2094
BmrFI CCNGG 3 cut(s) 158, 381, 954
BmrI ACTGGG 1 cut(s) 1071
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 2 cut(s) 53, 2481
BplI GAGNNNNNCTC 2 cut(s) 2328, 2360
BpmI CTGGAG 2 cut(s) 599, 1856
Bpu10I CCTNAGC 2 cut(s) 303, 2426
Bpu1102I GCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 2225
BpuEI CTTGAG 5 cut(s) 333, 813, 1385, 1649, 1986
Bsa29I ATCGAT 1 cut(s) 2370
BsaAI YACGTR 2 cut(s) 1335, 1825
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 2 cut(s) 107, 881
BsaXI ACNNNNNCTCC 2 cut(s) 2376, 2406
Bsc4I CCNNNNNNNGG 1 cut(s) 1748
Bse118I RCCGGY 1 cut(s) 1560
Bse1I ACTGG 5 cut(s) 582, 1066, 1105, 1275, 1581
Bse21I CCTNAGG 1 cut(s) 27
Bse3DI GCAATG 2 cut(s) 936, 1773
BseBI CCWGG 3 cut(s) 158, 381, 954
BseCI ATCGAT 1 cut(s) 2370
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 8 cut(s) 371, 1021, 1060, 1455, 1599, 1947, 2439, 2578
BseLI CCNNNNNNNGG 1 cut(s) 1748
BseMI GCAATG 2 cut(s) 936, 1773
BseMII CTCAG 5 cut(s) 18, 317, 815, 1054, 2715
BseNI ACTGG 5 cut(s) 582, 1066, 1105, 1275, 1581
BsePI GCGCGC 2 cut(s) 132, 273
BseRI GAGGAG 5 cut(s) 169, 666, 1748, 1877, 2618
BseYI CCCAGC 3 cut(s) 384, 491, 2095
BsgI GTGCAG 1 cut(s) 566
Bsh1236I CGCG 7 cut(s) 53, 134, 195, 261, 273, 275, 277
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 4 cut(s) 161, 702, 2093, 2424
BshVI ATCGAT 1 cut(s) 2370
BsiEI CGRYCG 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 3 cut(s) 108, 882, 1561
BslFI GGGAC 2 cut(s) 1860, 2432
BslI CCNNNNNNNGG 1 cut(s) 1748
BsmAI GTCTC 2 cut(s) 1479, 2637
BsmFI GGGAC 2 cut(s) 1860, 2432
BsmI GAATGC 2 cut(s) 740, 1597
BsnI GGCC 4 cut(s) 161, 702, 2093, 2424
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 2225
Bsp1286I GDGCHC 1 cut(s) 391
Bsp143I GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
Bsp1720I GCTNAGC 1 cut(s) 801
BspACI CCGC 9 cut(s) 144, 154, 193, 226, 246, 462, 624, 1649, 2194
BspANI GGCC 4 cut(s) 161, 702, 2093, 2424
BspCNI CTCAG 5 cut(s) 19, 316, 814, 1053, 2716
BspDI ATCGAT 1 cut(s) 2370
BspFNI CGCG 7 cut(s) 53, 134, 195, 261, 273, 275, 277
BspLI GGNNCC 1 cut(s) 2094
BspMAI CTGCAG 2 cut(s) 949, 1165
BspPI GGATC 2 cut(s) 371, 2342
BspT104I TTCGAA 1 cut(s) 2225
BsrDI GCAATG 2 cut(s) 936, 1773
BsrFI RCCGGY 1 cut(s) 1560
BsrI ACTGG 5 cut(s) 582, 1066, 1105, 1275, 1581
BssAI RCCGGY 1 cut(s) 1560
BssECI CCNNGG 1 cut(s) 229
BssHII GCGCGC 2 cut(s) 132, 273
BssMI GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
BssNAI GTATAC 2 cut(s) 887, 1129
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 210, 696
Bst1107I GTATAC 2 cut(s) 887, 1129
Bst2BI CACGAG 2 cut(s) 210, 696
Bst2UI CCWGG 3 cut(s) 158, 381, 954
Bst4CI ACNGT 8 cut(s) 688, 996, 1133, 1442, 1671, 1970, 2115, 2446
Bst6I CTCTTC 3 cut(s) 1026, 1477, 2593
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 438
BstBAI YACGTR 2 cut(s) 1335, 1825
BstBI TTCGAA 1 cut(s) 2225
BstC8I GCNNGC 7 cut(s) 134, 265, 275, 285, 704, 1500, 1635
BstF5I GGATG 8 cut(s) 371, 1021, 1060, 1455, 1599, 1947, 2439, 2578
BstFNI CGCG 7 cut(s) 53, 134, 195, 261, 273, 275, 277
BstHHI GCGC 6 cut(s) 134, 136, 197, 263, 275, 277
BstKTI GATC 9 cut(s) 128, 379, 837, 1678, 1849, 2137, 2350, 2401, 2562
BstMAI GTCTC 2 cut(s) 1479, 2637
BstMBI GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
BstMCI CGRYCG 1 cut(s) 226
BstNI CCWGG 3 cut(s) 158, 381, 954
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 3 cut(s) 156, 379, 952
BstSFI CTRYAG 6 cut(s) 945, 1161, 1327, 1535, 2043, 2500
BstUI CGCG 7 cut(s) 53, 134, 195, 261, 273, 275, 277
BstV2I GAAGAC 2 cut(s) 53, 2481
BstX2I RGATCY 2 cut(s) 125, 2398
BstXI CCANNNNNNTGG 1 cut(s) 2103
BstYI RGATCY 2 cut(s) 125, 2398
BstZ17I GTATAC 2 cut(s) 887, 1129
Bsu15I ATCGAT 1 cut(s) 2370
Bsu36I CCTNAGG 1 cut(s) 27
BsuI GTATCC 2 cut(s) 337, 2151
BsuRI GGCC 4 cut(s) 161, 702, 2093, 2424
BsuTUI ATCGAT 1 cut(s) 2370
BtgZI GCGATG 1 cut(s) 2433
BtrI CACGTC 1 cut(s) 2440
BtsCI GGATG 8 cut(s) 371, 1021, 1060, 1455, 1599, 1947, 2439, 2578
BtsI GCAGTG 2 cut(s) 297, 894
BtsIMutI CAGTG 7 cut(s) 297, 363, 894, 1447, 1588, 1667, 2111
Cac8I GCNNGC 7 cut(s) 134, 265, 275, 285, 704, 1500, 1635
CaiI CAGNNNCTG 3 cut(s) 953, 1684, 2450
CfoI GCGC 6 cut(s) 134, 136, 197, 263, 275, 277
Cfr10I RCCGGY 1 cut(s) 1560
Cfr13I GGNCC 4 cut(s) 950, 1651, 2092, 2202
ClaI ATCGAT 1 cut(s) 2370
CseI GACGC 2 cut(s) 267, 279
Csp6I GTAC 5 cut(s) 337, 527, 575, 1332, 2340
CspCI CAANNNNNGTGG 2 cut(s) 1495, 1530
CviAII CATG 8 cut(s) 284, 392, 977, 1075, 1883, 2206, 2380, 2641
CviQI GTAC 5 cut(s) 337, 527, 575, 1332, 2340
DpnI GATC 9 cut(s) 127, 378, 836, 1677, 1848, 2136, 2349, 2400, 2561
DpnII GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
DraI TTTAAA 1 cut(s) 571
DraIII CACNNNGTG 1 cut(s) 1673
DrdI GACNNNNNNGTC 1 cut(s) 2444
DseDI GACNNNNNNGTC 1 cut(s) 2444
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 3 cut(s) 1026, 1477, 2593
EarI CTCTTC 3 cut(s) 1026, 1477, 2593
EciI GGCGGA 3 cut(s) 133, 143, 477
Eco24I GRGCYC 1 cut(s) 391
Eco47I GGWCC 3 cut(s) 950, 1651, 2202
Eco57I CTGAAG 5 cut(s) 552, 1254, 1706, 2331, 2472
Eco81I CCTNAGG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 950
EcoRI GAATTC 1 cut(s) 904
EcoRII CCWGG 3 cut(s) 156, 379, 952
EcoT22I ATGCAT 3 cut(s) 740, 1171, 1599
EcoT38I GRGCYC 1 cut(s) 391
FaeI CATG 8 cut(s) 287, 395, 980, 1078, 1886, 2209, 2383, 2644
FalI AAGNNNNNCTT 8 cut(s) 438, 470, 1501, 1533, 1851, 1883, 2155, 2187
FaqI GGGAC 2 cut(s) 1860, 2432
FatI CATG 8 cut(s) 283, 391, 976, 1074, 1882, 2205, 2379, 2640
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 2 cut(s) 1675, 1846
FblI GTMKAC 3 cut(s) 886, 1128, 2059
FokI GGATG 8 cut(s) 358, 1028, 1067, 1462, 1606, 1934, 2446, 2585
FriOI GRGCYC 1 cut(s) 391
FspBI CTAG 2 cut(s) 1460, 1926
GlaI GCGC 6 cut(s) 133, 135, 196, 262, 274, 276
GsaI CCCAGC 3 cut(s) 388, 495, 2099
GsuI CTGGAG 2 cut(s) 599, 1856
HaeIII GGCC 4 cut(s) 161, 702, 2093, 2424
HapII CCGG 3 cut(s) 108, 882, 1561
HgaI GACGC 2 cut(s) 267, 279
HhaI GCGC 6 cut(s) 134, 136, 197, 263, 275, 277
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 287, 395, 980, 1078, 1886, 2209, 2383, 2644
Hin6I GCGC 6 cut(s) 132, 134, 195, 261, 273, 275
HinP1I GCGC 6 cut(s) 132, 134, 195, 261, 273, 275
HincII GTYRAC 1 cut(s) 2060
HindII GTYRAC 1 cut(s) 2060
HindIII AAGCTT 1 cut(s) 584
HpaII CCGG 3 cut(s) 108, 882, 1561
HphI GGTGA 2 cut(s) 415, 1702
Hpy166II GTNNAC 4 cut(s) 887, 1129, 2060, 2342
Hpy8I GTNNAC 4 cut(s) 887, 1129, 2060, 2342
Hpy99I CGWCG 3 cut(s) 10, 261, 273
HpyCH4III ACNGT 8 cut(s) 688, 996, 1133, 1442, 1671, 1970, 2115, 2446
HpyCH4IV ACGT 8 cut(s) 5, 202, 769, 1334, 1824, 2074, 2215, 2439
HpySE526I ACGT 8 cut(s) 5, 202, 769, 1334, 1824, 2074, 2215, 2439
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 287, 395, 980, 1078, 1886, 2209, 2383, 2644
HspAI GCGC 6 cut(s) 132, 134, 195, 261, 273, 275
Ksp22I TGATCA 2 cut(s) 1675, 1846
Kzo9I GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
LmnI GCTCC 6 cut(s) 146, 307, 517, 1864, 2245, 2405
MaeI CTAG 2 cut(s) 1460, 1926
MaeII ACGT 8 cut(s) 5, 202, 769, 1334, 1824, 2074, 2215, 2439
MaeIII GTNAC 6 cut(s) 1057, 1258, 1665, 1690, 2109, 2440
MalI GATC 9 cut(s) 127, 378, 836, 1677, 1848, 2136, 2349, 2400, 2561
MauBI CGCGCGCG 1 cut(s) 273
MboI GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
MflI RGATCY 2 cut(s) 125, 2398
MhlI GDGCHC 1 cut(s) 391
MlsI TGGCCA 1 cut(s) 161
MluNI TGGCCA 1 cut(s) 161
MlyI GAGTC 3 cut(s) 64, 81, 100
MmeI TCCRAC 2 cut(s) 280, 2164
Mox20I TGGCCA 1 cut(s) 161
Mph1103I ATGCAT 3 cut(s) 740, 1171, 1599
MscI TGGCCA 1 cut(s) 161
MslI CAYNNNNRTG 1 cut(s) 18
Msp20I TGGCCA 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 1889
MspI CCGG 3 cut(s) 108, 882, 1561
MspR9I CCNGG 3 cut(s) 158, 381, 954
Mva1269I GAATGC 2 cut(s) 740, 1597
MvaI CCWGG 3 cut(s) 158, 381, 954
MvnI CGCG 7 cut(s) 53, 134, 195, 261, 273, 275, 277
NdeII GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
NlaIII CATG 8 cut(s) 287, 395, 980, 1078, 1886, 2209, 2383, 2644
NlaIV GGNNCC 1 cut(s) 2094
NmuCI GTSAC 6 cut(s) 1057, 1258, 1665, 1690, 2109, 2440
NsiI ATGCAT 3 cut(s) 740, 1171, 1599
NspI RCATGY 1 cut(s) 287
NspV TTCGAA 1 cut(s) 2225
PaeI GCATGC 1 cut(s) 287
PalAI GGCGCGCC 1 cut(s) 132
PauI GCGCGC 2 cut(s) 132, 273
PcsI WCGNNNNNNNCGW 3 cut(s) 208, 265, 703
PctI GAATGC 2 cut(s) 740, 1597
PfeI GAWTC 7 cut(s) 550, 620, 878, 1681, 1814, 2084, 2717
PflFI GACNNNGTC 1 cut(s) 1490
PflMI CCANNNNNTGG 1 cut(s) 1748
PleI GAGTC 3 cut(s) 63, 80, 99
PpsI GAGTC 3 cut(s) 63, 80, 99
Ppu21I YACGTR 2 cut(s) 1335, 1825
PpuMI RGGWCCY 1 cut(s) 950
PshBI ATTAAT 1 cut(s) 1358
PsiI TTATAA 3 cut(s) 1115, 1920, 2283
Psp1406I AACGTT 1 cut(s) 769
Psp5II RGGWCCY 1 cut(s) 950
Psp6I CCWGG 3 cut(s) 156, 379, 952
PspFI CCCAGC 3 cut(s) 384, 491, 2095
PspGI CCWGG 3 cut(s) 156, 379, 952
PspN4I GGNNCC 1 cut(s) 2094
PspPI GGNCC 4 cut(s) 950, 1651, 2092, 2202
PspPPI RGGWCCY 1 cut(s) 950
PstI CTGCAG 2 cut(s) 949, 1165
PstNI CAGNNNCTG 3 cut(s) 953, 1684, 2450
PsuI RGATCY 2 cut(s) 125, 2398
PsyI GACNNNGTC 1 cut(s) 1490
PteI GCGCGC 2 cut(s) 132, 273
PvuII CAGCTG 1 cut(s) 1889
RsaI GTAC 5 cut(s) 338, 528, 576, 1333, 2341
RsaNI GTAC 5 cut(s) 337, 527, 575, 1332, 2340
RseI CAYNNNNRTG 1 cut(s) 18
SalI GTCGAC 1 cut(s) 2058
Sau3AI GATC 9 cut(s) 125, 376, 834, 1675, 1846, 2134, 2347, 2398, 2559
Sau96I GGNCC 4 cut(s) 950, 1651, 2092, 2202
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 3 cut(s) 64, 81, 100
ScrFI CCNGG 3 cut(s) 158, 381, 954
SduI GDGCHC 1 cut(s) 391
SfcI CTRYAG 6 cut(s) 945, 1161, 1327, 1535, 2043, 2500
SfuI TTCGAA 1 cut(s) 2225
SgsI GGCGCGCC 1 cut(s) 132
SinI GGWCC 3 cut(s) 950, 1651, 2202
SmiMI CAYNNNNRTG 1 cut(s) 18
SmlI CTYRAG 5 cut(s) 348, 828, 1364, 1628, 2001
SmoI CTYRAG 5 cut(s) 348, 828, 1364, 1628, 2001
SphI GCATGC 1 cut(s) 287
SsiI CCGC 9 cut(s) 144, 154, 193, 226, 246, 462, 624, 1649, 2194
SspMI CTAG 2 cut(s) 1460, 1926
StyD4I CCNGG 3 cut(s) 156, 379, 952
TaaI ACNGT 8 cut(s) 688, 996, 1133, 1442, 1671, 1970, 2115, 2446
TaiI ACGT 8 cut(s) 8, 205, 772, 1337, 1827, 2077, 2218, 2442
TaqI TCGA 9 cut(s) 1566, 2059, 2080, 2185, 2225, 2346, 2370, 2596, 2720
TatI WGTACW 2 cut(s) 336, 574
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 7 cut(s) 550, 620, 878, 1681, 1814, 2084, 2717
TscAI CASTG 7 cut(s) 304, 363, 901, 1447, 1588, 1674, 2118
TseFI GTSAC 6 cut(s) 1057, 1258, 1665, 1690, 2109, 2440
Tsp45I GTSAC 6 cut(s) 1057, 1258, 1665, 1690, 2109, 2440
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 7 cut(s) 304, 363, 901, 1447, 1588, 1674, 2118
Tth111I GACNNNGTC 1 cut(s) 1490
Van91I CCANNNNNTGG 1 cut(s) 1748
VpaK11BI GGWCC 3 cut(s) 950, 1651, 2202
VspI ATTAAT 1 cut(s) 1358
XapI RAATTY 5 cut(s) 99, 465, 904, 2026, 2542
XceI RCATGY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 2103
XmiI GTMKAC 3 cut(s) 886, 1128, 2059
XspI CTAG 2 cut(s) 1460, 1926
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 576
Zsp2I ATGCAT 3 cut(s) 740, 1171, 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.