Rh2CG490100

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000444
Physical Location & Seq
Reverse (-)
65670707 .. 65676210
5504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG490100.1

Sequence Viewer

Length: 2703 bp
ATGAGGCGTCGGGGAATCTGGAGTACGATTTCTATTGGAACCCAAAAAAGTGAAGGCAGAGACCATTGTGAGATGACATCTTTGGAGGGTTTTGAGCCCATAGCTATTTCCCCACAGAAACAAGACCCAGGATGGAAACACTGTCAACTTTACATAAAAACTGGGGACCCAAAAGTTGAGTTAAAGAAGTGTTTATATTGTGGCAAAGTGTTTCAGGGTGGAGGGATTAATAGGTTTAAATCTCACCTTGCTGGTAGAAAAGGAAATGGTCCCATTTGTGATCAGGTCCCTCCTCATGTTCGAGTTTCCATGTTGCAGTGCTTAGATGAAAAAATTGCATCTTTAAGGCACAGAAAGAGTCAAATGGCTGTGAACCCGAGTCGTAGTTTTAGTGAACTAGACAATGTGTTTGCTGAAAATGAAGTTGGAGGGCCCAATTTGATGAATCAAGAGGAAGATGTGGGAATGAGTGTTGATAATGGGATTAGGGTTGGTGATAATCTGACTGATGTGCCTCTTGAGATTGATGATTGTTTTAGGAGTGATGGTCTAGAGGGATTGACTACTGTGCCTCTTGCAGCAGTTGACTCGTATAAGGGTAATGCTCTAGGGAGAAAAGCTGGAGTACCGAATCACAGTTTTTCGGAAAATCGAGAGGTGGGTGTTGATAGACGAGTAGGGTGGAGAGGCGGAACTTCTTTTGTAGCTACTACTGCTGGTTTCGGTTCTGGTTCTGCTAATGCTACTAAATTGAGTTATGTCCCAGCGCCTATTCGTAGTGAGGTAGACAAGCCATTTTACGAAAACACGCAAGGAAGATCAGTTGGTCATCCCAATTCATATTTGTTTGAAAATGAAACAGATGTTGGCACAAGTAATACAAATGGGAAGAACAGGAAGAGGGTAAGAGATGAAAATGGATCAATGGTTGCTAATGCTGGCACTATTGGAAATTATGGTGAAGTTGAAAAGGTGAACAGTCAGCAGATTCATATGGCAATAGGACGGTTCCTATATGAAATTCAGGCTCCTCTGGGTGCCGTGAAGAACTCTGTTTATTTTCAGCCAATGATTGATGCAATTGCTTCTGGGGGAATGGAGAGTAAACCACCCTCGTATCATGATCTTCGGGGTTGGATATTAAATAATGTGGTTGAAGAAGTAAAGAATGATATTCATCAGCACATGAATACTTGGGAAAGGAATGGTTGCTCTCTTCTAGTAAACCAATTGAACTCTGAAAAGGGTAGAATATTGCTAAATTTCTCAGTGTACAGTCCCGAAGGAACAACATATTTGAAATCTGTGGATGCTTCCTCATTTATAAATTCTCCAGATGCTTTGTATGAGATTCTTAAGCAAGTGTTGGAAGAAGTTGGAGTTGGGCGTGTTTTGCAAGTAATTACAAATAGTGAAGAGCACTATGTTGTTGCTGGGAAGAGGTTGATGGATACGTTTCCTACACTGTACTGGTCTCCCTGTGCTGCTGCTTGCATAAATCTGATACTTGAGGATTTTGGAAAAGTTGAGTGGATAAAATCTATTATAGAACAGGCTAGATCTGTTACAAGATTTATCTATAAACATGTCGTCATTCTGAATATGATGCGGAGGTATACTTTTGGAAATGACATTGTGAAGGTGGGAGTAACTCGGTTTGCTACAGACTTTATGACTTTGAAACAAATGGCTGACCTTAAATTCAATTTGCAGACTATGGTTACTTCAAAAGAATGGGAAGGGTGCCCTTATTCTCAAACATCAGAAGGATCAGCAATGCTGGATGTAATAAGTAATCACACATTTTGGTCTTCGTGCATTATGATCACCCGATTAACAAATCCGCTCCTGCGAGTTTTGAGAATTGTTGGTAGCCAGAAGAAGGCAGCAATGGGATATGTATTTGGAGGAATGTATCGAGCAAAAGAAACAATCAAGAGAGAACTTGTCAAGAAAGAAGAATATATGGCTTACTGGAATATTATAGATTACAGGTGGCAAAAATTATGGGATCTTCCTCTTCATGCTGCTGGTTTTTACCTTAATCCCAAGTTTTTCTATAGCATCAAGGGAGAAATGCACAAGGTGATTATGTCAAAAATGCTTGACTGCATAGAGAAATTGGTTCCAGATTTAAAAGTTCAAGATGAAATTAGCAAAGAGATAAACTTGTACAAAAATGCTGTTGGAGATCTGGGGAGGAATTTGGCCATTAGAGCTAGAGACACTCTGCTTCCCGCTGAATGGTGGTCTACATATGGCGGTGGTTGCCCAAATATGGCACGTTTTGCAGTTCATATTCTCAGTCAAACTTGCAGCTTAATTCAGTGCAAGGAGAATCAGATTCCTTTTGACCAACTGCACAAGACAAGGAACAGCTTAGAGCATCAACGGCTTAGTGATTTTGTCTTTGTTCAGTACAACTTGCAACTAAGACAGATGGTTCATAAGAACAAAGAGCATGAGTATGTGGATCCTATATCATTTGACAACACTAGCACTGTTGAAGACTGGATAACAGAGACTGAAATGTACCTAGAAGACAATGAAAATACTGATTGGAAGGCACTTGAACCGCCCTCTTATAATTCGAGGCTTTTAGAACTTTCAGTTGATGAAGCTGAAGACTTGGGTTCAGGGTTTGATGATAACGAGATTTTCTTAAACGGATTGCAATTGGTTAAAGAAGAAGGCAGAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

900

Amino Acids

101.66

Weight (kDa)

6.15

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 371 - 522 1.9e-55 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 732 - 809 4.7e-07 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1325, 2586
AccB1I GGYRCC 2 cut(s) 1037, 1743
AccBSI CCGCTC 1 cut(s) 1846
AccI GTMKAC 3 cut(s) 786, 1616, 2252
AciI CCGC 6 cut(s) 690, 1609, 1844, 2238, 2262, 2576
AclWI GGATC 5 cut(s) 928, 1777, 2019, 2468, 2481
AcoI YGGCCR 1 cut(s) 2208
AcsI RAATTY 5 cut(s) 1020, 1261, 1327, 1700, 2203
AcuI CTGAAG 1 cut(s) 2643
AcyI GRCGYC 1 cut(s) 7
AdeI CACNNNGTG 1 cut(s) 2086
AfaI GTAC 7 cut(s) 25, 627, 1274, 1469, 2174, 2420, 2534
AfiI CCNNNNNNNGG 3 cut(s) 1882, 2244, 2278
AflII CTTAAG 1 cut(s) 1355
AflIII ACRYGT 1 cut(s) 1585
AjnI CCWGG 1 cut(s) 127
AjuI GAANNNNNNNTTGG 6 cut(s) 408, 440, 849, 881, 1365, 1397
AluBI AGCT 7 cut(s) 104, 620, 707, 2219, 2319, 2379, 2621
AluI AGCT 7 cut(s) 104, 620, 707, 2219, 2319, 2379, 2621
Alw21I GWGCWC 1 cut(s) 1422
Alw26I GTCTC 4 cut(s) 54, 1479, 2217, 2516
AlwI GGATC 5 cut(s) 928, 1777, 2019, 2468, 2481
AlwNI CAGNNNCTG 1 cut(s) 2525
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 2 cut(s) 431, 2208
ApaI GGGCCC 1 cut(s) 435
ApeKI GCWGC 6 cut(s) 578, 1484, 1487, 1886, 2027, 2316
ApoI RAATTY 5 cut(s) 1020, 1261, 1327, 1700, 2203
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AseI ATTAAT 1 cut(s) 228
AspLEI GCGC 1 cut(s) 769
AspS9I GGNCC 5 cut(s) 166, 269, 286, 431, 432
AsuHPI GGTGA 6 cut(s) 236, 506, 971, 985, 1819, 2098
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 3 cut(s) 166, 269, 286
BaeGI GKGCMC 2 cut(s) 435, 1748
BaeI ACNNNNGTAYC 2 cut(s) 1100, 1133
BalI TGGCCA 1 cut(s) 2210
BamHI GGATCC 1 cut(s) 2473
BanI GGYRCC 2 cut(s) 1037, 1743
BanII GRGCYC 2 cut(s) 99, 435
BbsI GAAGAC 4 cut(s) 1803, 2514, 2547, 2631
Bbv12I GWGCWC 1 cut(s) 1422
BbvI GCAGC 6 cut(s) 590, 1471, 1474, 1898, 2014, 2328
BccI CCATC 4 cut(s) 126, 539, 1441, 2434
BceAI ACGGC 2 cut(s) 1025, 2408
BcgI CGANNNNNNTGC 2 cut(s) 570, 604
BciT130I CCWGG 1 cut(s) 129
BciVI GTATCC 1 cut(s) 1444
BclI TGATCA 2 cut(s) 280, 1824
BcoDI GTCTC 4 cut(s) 54, 1479, 2217, 2516
BfaI CTAG 8 cut(s) 398, 551, 608, 1220, 1557, 2220, 2496, 2537
BfmI CTRYAG 2 cut(s) 1662, 2059
BfoI RGCGCY 1 cut(s) 770
BfrI CTTAAG 1 cut(s) 1355
BfuI GTATCC 1 cut(s) 1444
BglII AGATCT 2 cut(s) 1559, 2191
BisI GCNGC 6 cut(s) 579, 1485, 1488, 1887, 2028, 2317
BlsI GCNGC 6 cut(s) 580, 1486, 1489, 1888, 2029, 2318
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 3 cut(s) 166, 269, 286
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 5 cut(s) 166, 269, 286, 431, 432
BmrFI CCNGG 1 cut(s) 129
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 7 cut(s) 347, 1066, 1300, 1327, 1596, 2073, 2395
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 4 cut(s) 1803, 2514, 2547, 2631
BpmI CTGGAG 3 cut(s) 40, 642, 1317
BpuEI CTTGAG 2 cut(s) 539, 1529
BsaBI GATNNNNATC 1 cut(s) 1176
BsaHI GRCGYC 1 cut(s) 7
BsaI GGTCTC 2 cut(s) 54, 1479
BsaJI CCNNGG 1 cut(s) 127
BsaXI ACNNNNNCTCC 2 cut(s) 532, 562
Bsc4I CCNNNNNNNGG 3 cut(s) 1882, 2244, 2278
Bse1I ACTGG 4 cut(s) 166, 1475, 1979, 2516
Bse3DI GCAATG 2 cut(s) 1782, 1896
Bse8I GATNNNNATC 1 cut(s) 1176
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 127
BseGI GGATG 4 cut(s) 137, 829, 1315, 1789
BseJI GATNNNNATC 1 cut(s) 1176
BseLI CCNNNNNNNGG 3 cut(s) 1882, 2244, 2278
BseMI GCAATG 2 cut(s) 1782, 1896
BseMII CTCAG 2 cut(s) 1281, 2317
BseNI ACTGG 4 cut(s) 166, 1475, 1979, 2516
BseRI GAGGAG 2 cut(s) 282, 1020
BseSI GKGCMC 2 cut(s) 435, 1748
BseXI GCAGC 6 cut(s) 590, 1471, 1474, 1898, 2014, 2328
BseYI CCCAGC 2 cut(s) 763, 1433
BsgI GTGCAG 1 cut(s) 2345
BshFI GGCC 2 cut(s) 433, 2210
BshNI GGYRCC 2 cut(s) 1037, 1743
BsiHKAI GWGCWC 1 cut(s) 1422
BsiHKCI CYCGRG 1 cut(s) 376
BslFI GGGAC 5 cut(s) 179, 255, 272, 746, 1263
BslI CCNNNNNNNGG 3 cut(s) 1882, 2244, 2278
BsmAI GTCTC 4 cut(s) 54, 1479, 2217, 2516
BsmFI GGGAC 5 cut(s) 179, 255, 272, 746, 1263
BsnI GGCC 2 cut(s) 433, 2210
Bso31I GGTCTC 2 cut(s) 54, 1479
BsoBI CYCGRG 1 cut(s) 376
Bsp120I GGGCCC 1 cut(s) 431
Bsp1286I GDGCHC 4 cut(s) 99, 435, 1422, 1748
Bsp1407I TGTACA 2 cut(s) 1272, 2172
BspACI CCGC 6 cut(s) 690, 1609, 1844, 2238, 2262, 2576
BspANI GGCC 2 cut(s) 433, 2210
BspCNI CTCAG 2 cut(s) 1280, 2316
BspHI TCATGA 1 cut(s) 1120
BspPI GGATC 5 cut(s) 928, 1777, 2019, 2468, 2481
BspQI GCTCTTC 1 cut(s) 1410
BspT107I GGYRCC 2 cut(s) 1037, 1743
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 2 cut(s) 54, 1479
BsrBI CCGCTC 1 cut(s) 1846
BsrDI GCAATG 2 cut(s) 1782, 1896
BsrGI TGTACA 2 cut(s) 1272, 2172
BsrI ACTGG 4 cut(s) 166, 1475, 1979, 2516
BssECI CCNNGG 1 cut(s) 127
BssNAI GTATAC 1 cut(s) 1617
BssNI GRCGYC 1 cut(s) 7
Bst1107I GTATAC 1 cut(s) 1617
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 8 cut(s) 143, 568, 638, 980, 1008, 1277, 1467, 2503
Bst6I CTCTTC 5 cut(s) 893, 1221, 1410, 1433, 2025
BstACI GRCGYC 1 cut(s) 7
BstAFI CTTAAG 1 cut(s) 1355
BstAPI GCANNNNNTGC 1 cut(s) 2288
BstAUI TGTACA 2 cut(s) 1272, 2172
BstC8I GCNNGC 2 cut(s) 940, 1492
BstDEI CTNAG 6 cut(s) 322, 1267, 2303, 2380, 2396, 2432
BstF5I GGATG 4 cut(s) 137, 829, 1315, 1789
BstH2I RGCGCY 1 cut(s) 770
BstHHI GCGC 1 cut(s) 769
BstMAI GTCTC 4 cut(s) 54, 1479, 2217, 2516
BstMWI GCNNNNNNNGC 6 cut(s) 713, 1393, 2216, 2268, 2288, 2392
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 1589
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 2 cut(s) 1662, 2059
BstSLI GKGCMC 2 cut(s) 435, 1748
BstV1I GCAGC 6 cut(s) 590, 1471, 1474, 1898, 2014, 2328
BstV2I GAAGAC 4 cut(s) 1803, 2514, 2547, 2631
BstX2I RGATCY 4 cut(s) 1559, 2011, 2191, 2473
BstYI RGATCY 4 cut(s) 1559, 2011, 2191, 2473
BstZ17I GTATAC 1 cut(s) 1617
BsuI GTATCC 1 cut(s) 1444
BsuRI GGCC 2 cut(s) 433, 2210
BtsCI GGATG 4 cut(s) 137, 829, 1315, 1789
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 6 cut(s) 139, 323, 1275, 1463, 2333, 2499
Cac8I GCNNGC 2 cut(s) 940, 1492
CaiI CAGNNNCTG 1 cut(s) 2525
CciI TCATGA 1 cut(s) 1120
CfoI GCGC 1 cut(s) 769
Cfr13I GGNCC 5 cut(s) 166, 269, 286, 431, 432
Csp6I GTAC 7 cut(s) 24, 626, 1273, 1468, 2173, 2419, 2533
CviAII CATG 7 cut(s) 296, 310, 1121, 1186, 1586, 2024, 2462
CviQI GTAC 7 cut(s) 24, 626, 1273, 1468, 2173, 2419, 2533
DdeI CTNAG 6 cut(s) 322, 1267, 2303, 2380, 2396, 2432
DraI TTTAAA 2 cut(s) 238, 2136
DraIII CACNNNGTG 1 cut(s) 2086
EaeI YGGCCR 1 cut(s) 2208
Eam1104I CTCTTC 5 cut(s) 893, 1221, 1410, 1433, 2025
EarI CTCTTC 5 cut(s) 893, 1221, 1410, 1433, 2025
EciI GGCGGA 1 cut(s) 705
Eco24I GRGCYC 2 cut(s) 99, 435
Eco31I GGTCTC 2 cut(s) 54, 1479
Eco47I GGWCC 3 cut(s) 166, 269, 286
Eco57I CTGAAG 1 cut(s) 2643
Eco88I CYCGRG 1 cut(s) 376
EcoO109I RGGNCCY 3 cut(s) 166, 286, 431
EcoRII CCWGG 1 cut(s) 127
EcoT38I GRGCYC 2 cut(s) 99, 435
FaeI CATG 7 cut(s) 299, 313, 1124, 1189, 1589, 2027, 2465
FaqI GGGAC 5 cut(s) 179, 255, 272, 746, 1263
FatI CATG 7 cut(s) 295, 309, 1120, 1185, 1585, 2023, 2461
FauI CCCGC 1 cut(s) 2245
FauNDI CATATG 2 cut(s) 993, 2257
FbaI TGATCA 2 cut(s) 280, 1824
FblI GTMKAC 3 cut(s) 786, 1616, 2252
Fnu4HI GCNGC 6 cut(s) 579, 1485, 1488, 1887, 2028, 2317
FokI GGATG 4 cut(s) 144, 816, 1322, 1796
FriOI GRGCYC 2 cut(s) 99, 435
Fsp4HI GCNGC 6 cut(s) 579, 1485, 1488, 1887, 2028, 2317
FspBI CTAG 8 cut(s) 398, 551, 608, 1220, 1557, 2220, 2496, 2537
GlaI GCGC 1 cut(s) 768
GluI GCNGC 6 cut(s) 579, 1485, 1488, 1887, 2028, 2317
GsaI CCCAGC 2 cut(s) 767, 1437
GsuI CTGGAG 3 cut(s) 40, 642, 1317
HaeII RGCGCY 1 cut(s) 770
HaeIII GGCC 2 cut(s) 433, 2210
HhaI GCGC 1 cut(s) 769
Hin1I GRCGYC 1 cut(s) 7
Hin1II CATG 7 cut(s) 299, 313, 1124, 1189, 1589, 2027, 2465
Hin6I GCGC 1 cut(s) 767
HinP1I GCGC 1 cut(s) 767
HincII GTYRAC 2 cut(s) 146, 586
HindII GTYRAC 2 cut(s) 146, 586
HphI GGTGA 6 cut(s) 236, 506, 971, 985, 1819, 2098
Hpy188I TCNGA 7 cut(s) 504, 646, 1240, 1503, 1599, 1765, 2343
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 8 cut(s) 47, 1277, 1633, 1733, 1760, 1876, 2557, 2684
HpyCH4III ACNGT 8 cut(s) 143, 568, 638, 980, 1008, 1277, 1467, 2503
HpyCH4IV ACGT 2 cut(s) 1454, 2284
HpyF10VI GCNNNNNNNGC 6 cut(s) 713, 1393, 2216, 2268, 2288, 2392
HpyF3I CTNAG 6 cut(s) 322, 1267, 2303, 2380, 2396, 2432
HpySE526I ACGT 2 cut(s) 1454, 2284
Hsp92I GRCGYC 1 cut(s) 7
Hsp92II CATG 7 cut(s) 299, 313, 1124, 1189, 1589, 2027, 2465
HspAI GCGC 1 cut(s) 767
KflI GGGWCCC 1 cut(s) 166
Ksp22I TGATCA 2 cut(s) 280, 1824
LguI GCTCTTC 1 cut(s) 1410
LmnI GCTCC 2 cut(s) 1033, 1851
Lsp1109I GCAGC 6 cut(s) 590, 1471, 1474, 1898, 2014, 2328
LweI GCATC 7 cut(s) 347, 1066, 1300, 1327, 1596, 2073, 2395
MaeI CTAG 8 cut(s) 398, 551, 608, 1220, 1557, 2220, 2496, 2537
MaeII ACGT 2 cut(s) 1454, 2284
MaeIII GTNAC 3 cut(s) 1564, 1648, 1720
MbiI CCGCTC 1 cut(s) 1846
MfeI CAATTG 3 cut(s) 1080, 1229, 2675
MflI RGATCY 4 cut(s) 1559, 2011, 2191, 2473
MhlI GDGCHC 4 cut(s) 99, 435, 1422, 1748
MlsI TGGCCA 1 cut(s) 2210
MluNI TGGCCA 1 cut(s) 2210
MlyI GAGTC 3 cut(s) 367, 388, 581
MmeI TCCRAC 5 cut(s) 406, 1115, 1347, 1357, 2167
Mox20I TGGCCA 1 cut(s) 2210
MscI TGGCCA 1 cut(s) 2210
MslI CAYNNNNRTG 1 cut(s) 2466
Msp20I TGGCCA 1 cut(s) 2210
MspA1I CMGCKG 1 cut(s) 2240
MspCI CTTAAG 1 cut(s) 1355
MspR9I CCNGG 1 cut(s) 129
MunI CAATTG 3 cut(s) 1080, 1229, 2675
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 6 cut(s) 713, 1393, 2216, 2268, 2288, 2392
NdeI CATATG 2 cut(s) 993, 2257
NlaIII CATG 7 cut(s) 299, 313, 1124, 1189, 1589, 2027, 2465
NspI RCATGY 1 cut(s) 1589
PagI TCATGA 1 cut(s) 1120
PciI ACATGT 1 cut(s) 1585
PciSI GCTCTTC 1 cut(s) 1410
PfeI GAWTC 7 cut(s) 15, 445, 631, 988, 1351, 2338, 2344
PkrI GCNGC 6 cut(s) 580, 1486, 1489, 1888, 2029, 2318
PleI GAGTC 3 cut(s) 366, 387, 581
PpsI GAGTC 3 cut(s) 366, 387, 581
PpuMI RGGWCCY 2 cut(s) 166, 286
PscI ACATGT 1 cut(s) 1585
PshBI ATTAAT 1 cut(s) 228
PsiI TTATAA 2 cut(s) 1325, 2586
Psp5II RGGWCCY 2 cut(s) 166, 286
Psp6I CCWGG 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 763, 1433
PspGI CCWGG 1 cut(s) 127
PspOMI GGGCCC 1 cut(s) 431
PspPI GGNCC 5 cut(s) 166, 269, 286, 431, 432
PspPPI RGGWCCY 2 cut(s) 166, 286
PstNI CAGNNNCTG 1 cut(s) 2525
PsuI RGATCY 4 cut(s) 1559, 2011, 2191, 2473
RsaI GTAC 7 cut(s) 25, 627, 1274, 1469, 2174, 2420, 2534
RsaNI GTAC 7 cut(s) 24, 626, 1273, 1468, 2173, 2419, 2533
RseI CAYNNNNRTG 1 cut(s) 2466
SapI GCTCTTC 1 cut(s) 1410
SatI GCNGC 6 cut(s) 579, 1485, 1488, 1887, 2028, 2317
Sau96I GGNCC 5 cut(s) 166, 269, 286, 431, 432
SchI GAGTC 3 cut(s) 367, 388, 581
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 4 cut(s) 99, 435, 1422, 1748
SfaNI GCATC 7 cut(s) 347, 1066, 1300, 1327, 1596, 2073, 2395
SfcI CTRYAG 2 cut(s) 1662, 2059
SinI GGWCC 3 cut(s) 166, 269, 286
SmiMI CAYNNNNRTG 1 cut(s) 2466
SmlI CTYRAG 3 cut(s) 518, 1355, 1508
SmoI CTYRAG 3 cut(s) 518, 1355, 1508
SsiI CCGC 6 cut(s) 690, 1609, 1844, 2238, 2262, 2576
SspI AATATT 2 cut(s) 1254, 1981
SspMI CTAG 8 cut(s) 398, 551, 608, 1220, 1557, 2220, 2496, 2537
StyD4I CCNGG 1 cut(s) 127
TaaI ACNGT 8 cut(s) 143, 568, 638, 980, 1008, 1277, 1467, 2503
TaiI ACGT 2 cut(s) 1457, 2287
TaqI TCGA 4 cut(s) 301, 652, 1918, 2591
TatI WGTACW 4 cut(s) 1272, 1467, 2172, 2418
TfiI GAWTC 7 cut(s) 15, 445, 631, 988, 1351, 2338, 2344
TscAI CASTG 6 cut(s) 146, 323, 1275, 1470, 2333, 2506
TseI GCWGC 6 cut(s) 578, 1484, 1487, 1886, 2027, 2316
TspGWI ACGGA 1 cut(s) 2682
TspRI CASTG 6 cut(s) 146, 323, 1275, 1470, 2333, 2506
Vha464I CTTAAG 1 cut(s) 1355
VpaK11BI GGWCC 3 cut(s) 166, 269, 286
VspI ATTAAT 1 cut(s) 228
XapI RAATTY 5 cut(s) 1020, 1261, 1327, 1700, 2203
XbaI TCTAGA 1 cut(s) 550
XceI RCATGY 1 cut(s) 1589
XmiI GTMKAC 3 cut(s) 786, 1616, 2252
XspI CTAG 8 cut(s) 398, 551, 608, 1220, 1557, 2220, 2496, 2537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.