MD01G1137200.v1.1

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000444
Physical Location & Seq
Forward (+)
24627191 .. 24630874
3684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1137200.v1.1.491

Sequence Viewer

Length: 2280 bp
ATGGCTTCTGGTCTGGTAATGGAGCCCGTGCCGATAACGTCACAAAAGCACGACCCGGCATGGAAACACTGTCAAATGTTTAAGATTGGGGAGAGGGTTCAGCTGAAGTGCATATATTGTAACAAATTGTTTAAGGGTGGTGGGATTCATAGGATTAAGGAACACCTTGCCGGTCAGAAGGGTAATGCGTCGACTTGTCTGAGAGTTCCGCCGGATGTTCGGGCTCAAATGCAGCAGAGTCTAGATGGGGTAGTGCTGAAGAAGAGGAATAGGCAGAAGCTTGATGAGGAGATTACTAACATTAATCCCTCACCTCATGGTGAGGCAGAGCCGATTGCTGATCAGAGTGATGTGAGTAATGGGGGTCAGTTGATTGGAGTTCCCGAGGCGCTTGAGCACAATTCGGGGTTGTTGGGGAACCAAGAAGGAATGACAAGTGGGAGGAGTTTGGGGAGAAGGAAAAGGGGGAGAGGCAAAAGTTCTTTTGCAGGACATAGTGCTCTTGTTGTTAGTAATAGTGCTGCTTTAGGTTCTCCAAAGGTGAACAATTTTGTTCACGAGGCAATTGGAAGGTTTTTGTATGATATTGGGGCGCCTCCAGATGCAGTGAACTCTGCGTATTTCCAACCGATGATTGATGCAATTGCTTCAGGTGGTTCAAGGGTTGTGCCTCCTACGTACCATGATATTCGTAGTTGGATATTGAAGAATTCAGTTGAAGAAGTGAGGAACAGTATTGATAAACATAGGGAAACGTGGGGAAGAACGGGTTGTTCAGTCTTGGTCGATCAGTGGAACACAGAGTCAGGTAACGTTTTGCTAAGTTTTTTGGTGTACTGTCCTGAAGGAACAGTTTTTTGGGAATCTGTTGATGCATCAGACATTATAAATTCCCCGGATGCGCTTTACGAACTGCTTAGGAGAGTGGTAGAGGAAGTTGGAGTAAAAGATGTGCTGCAAGTGATTACTAGTAGTGAAGAGCAATATATGGTTGCGGGGAGGAGGTTGACTGATACTTTCCCTGCGCTATATTGGACTCCATGTGCAGCTCGGTGCTTAGATTTAATACTCGAAGATTTTGGTAATATTGAGTGGATAAATACGGTCATTGAACAAGCAAGATCCATTACAAAGTTTGTGTACAATCATAGTGCAGTGTTGAATATGGTCAGAAGGTCTACTTTTGGCAATGATATAGTAGAACCAGGCGCCACTCGCTTCGCTACAAACTTTACAACATTAAAACGACTGGTTGATCTAAAACACTGCGTACAGGTCATGGTTACTTCGCAGGAGTGGATGGACTCCCTATATTCAAAGGAACCGGGGGGACTGGAGATGTTGGACCTTGTTAATAATCAGTCATTTTGGTCCTCATGCATCTTGATTGTTCATCTGACAGATCCTCTCTTGCGAGTTTTGAGAATGGTTGGTAGTGAGAAGAGGCCTGCAATGGGATACGTGTATGCTGGAATGTATTGGGCAAAAGAAACAATTAAGAAAGAACTCATTAAGAGGGAGGAGTACATGGTATACTGGAATATCATAGATCAACGATGGGAACAGCTGTGGCATTCTCCTCTTTATTCTGCAGGCTTCTACCTTAACCCCAAAATATTTTACAGTATCAAAGGAGATATACATAGTGATATTGTCTCGGGTACTTTTGATTGCATCGAGCGATTGGTTCCTGATACAAATGTCCAAGATCAAATCATCAAAGAGCTAAATTTGTACAAGAGTGCTGCTGGAGATTTTCGAAGGAAGATGGCAATTAGGGCTAGAGACACTCTGCTTCCTGCTGAATGGTGGTCAACATATGGAGGAGGTTGCCCAAATTTGACACGCTTGGCAATCCGAATTCTCAGTCAAACTTGCAGTTCGATTGGCTGCAGGCGAAATGAGAATCCTTTTGGGAAAGCACACAACACAAGGAACTGCCTTGAGCGTCAACGTATCAGTGACCTCGTCTTTGTTCGTTATAACTTGCGACTGAAACAAATGGTTGGTAAGAACATCGGACAAGATGTTATGGATCCCATATCATTTGAAAACATTAGCATGACCGAAGACTGGGTTACCGGGAAGGACATGTGCTTAGATGACAATGGGAGTTCCGACTGGATGGAACTCGATTCAAACTCTGTAAGCACAATGCTTCTAGGGCCATCAAATGATGATGCCGATGACTTGGGCTCTGGGTTTGACGATTATGAGATCTTCAGTAGAGCAAAAGACGGTGAAGAGGAAAATGTTGAAGACAGCGTAGAAAATCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

760

Amino Acids

85.43

Weight (kDa)

5.71

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 225 - 376 3.8e-58 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 575 - 663 1.8e-11 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 887, 1983
AccB1I GGYRCC 2 cut(s) 592, 1208
AccI GTMKAC 3 cut(s) 191, 1178, 1533
AciI CCGC 2 cut(s) 209, 995
AclI AACGTT 1 cut(s) 813
AclWI GGATC 4 cut(s) 1116, 1397, 2030, 2043
AcsI RAATTY 5 cut(s) 709, 889, 1729, 1837, 1860
AcuI CTGAAG 5 cut(s) 125, 278, 633, 864, 2206
AcyI GRCGYC 2 cut(s) 593, 1209
AfaI GTAC 7 cut(s) 680, 836, 1142, 1272, 1526, 1663, 1736
AfiI CCNNNNNNNGG 2 cut(s) 1454, 2073
AflIII ACRYGT 2 cut(s) 1461, 2091
AgsI TTSAA 9 cut(s) 660, 706, 719, 1112, 1162, 1317, 2051, 2139, 2258
AhlI ACTAGT 1 cut(s) 968
AjnI CCWGG 1 cut(s) 1204
AjuI GAANNNNNNNTTGG 2 cut(s) 841, 873
AluBI AGCT 5 cut(s) 103, 280, 1049, 1567, 1726
AluI AGCT 5 cut(s) 103, 280, 1049, 1567, 1726
Alw21I GWGCWC 2 cut(s) 399, 502
Alw26I GTCTC 2 cut(s) 1660, 1779
AlwI GGATC 4 cut(s) 1116, 1397, 2030, 2043
Ama87I CYCGRG 2 cut(s) 383, 1657
AoxI GGCC 2 cut(s) 1445, 2165
ApeKI GCWGC 6 cut(s) 232, 521, 955, 1046, 1745, 1890
ApoI RAATTY 5 cut(s) 709, 889, 1729, 1837, 1860
ArsI GACNNNNNNTTYG 4 cut(s) 1852, 1884, 1955, 1987
AseI ATTAAT 1 cut(s) 303
AspLEI GCGC 5 cut(s) 391, 595, 904, 1027, 1211
AspS9I GGNCC 3 cut(s) 1345, 1371, 2165
AsuC2I CCSGG 4 cut(s) 56, 896, 1326, 2083
AsuHPI GGTGA 4 cut(s) 303, 332, 553, 2252
AsuII TTCGAA 1 cut(s) 1759
AvaI CYCGRG 2 cut(s) 383, 1657
AvaII GGWCC 2 cut(s) 1345, 1371
BaeI ACNNNNGTAYC 2 cut(s) 1686, 1719
BamHI GGATCC 1 cut(s) 2035
BanI GGYRCC 2 cut(s) 592, 1208
BanII GRGCYC 3 cut(s) 27, 226, 2198
BauI CACGAG 1 cut(s) 557
BbsI GAAGAC 2 cut(s) 2076, 2265
Bbv12I GWGCWC 2 cut(s) 399, 502
BbvI GCAGC 6 cut(s) 244, 508, 942, 1058, 1732, 1877
BccI CCATC 6 cut(s) 239, 1294, 1551, 1762, 2119, 2176
BciT130I CCWGG 1 cut(s) 1206
BciVI GTATCC 1 cut(s) 1451
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 4 cut(s) 56, 896, 1326, 2083
BcoDI GTCTC 2 cut(s) 1660, 1779
BcuI ACTAGT 1 cut(s) 968
BfaI CTAG 4 cut(s) 242, 969, 1782, 2162
BfmI CTRYAG 2 cut(s) 1590, 1891
BfoI RGCGCY 3 cut(s) 392, 596, 1212
BfuI GTATCC 1 cut(s) 1451
BglII AGATCT 1 cut(s) 2217
BisI GCNGC 6 cut(s) 233, 522, 956, 1047, 1746, 1891
BlsI GCNGC 6 cut(s) 234, 523, 957, 1048, 1747, 1892
Bme1390I CCNGG 5 cut(s) 56, 896, 1206, 1326, 2083
Bme18I GGWCC 2 cut(s) 1345, 1371
BmeT110I CYCGRG 2 cut(s) 383, 1657
BmgT120I GGNCC 3 cut(s) 1345, 1371, 2165
BmiI GGNNCC 7 cut(s) 24, 419, 594, 1210, 1323, 1689, 2037
BmrFI CCNGG 5 cut(s) 56, 896, 1206, 1326, 2083
BmrI ACTGGG 1 cut(s) 2083
BmsI GCATC 8 cut(s) 592, 628, 862, 884, 889, 1389, 1683, 2170
BmuI ACTGGG 1 cut(s) 2083
BpiI GAAGAC 2 cut(s) 2076, 2265
BpmI CTGGAG 3 cut(s) 582, 1355, 1770
Bpu10I CCTNAGC 1 cut(s) 917
Bpu14I TTCGAA 1 cut(s) 1759
BpuEI CTTGAG 2 cut(s) 413, 1964
BpuMI CCSGG 4 cut(s) 56, 896, 1326, 2083
BsaAI YACGTR 2 cut(s) 678, 1462
BsaHI GRCGYC 2 cut(s) 593, 1209
BsaJI CCNNGG 3 cut(s) 384, 894, 1325
Bsc4I CCNNNNNNNGG 2 cut(s) 1454, 2073
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 5 cut(s) 1254, 1338, 1541, 2078, 2126
Bse3DI GCAATG 2 cut(s) 1195, 1458
BseBI CCWGG 1 cut(s) 1206
BseDI CCNNGG 3 cut(s) 384, 894, 1325
BseGI GGATG 4 cut(s) 220, 904, 1305, 2130
BseLI CCNNNNNNNGG 2 cut(s) 1454, 2073
BseMI GCAATG 2 cut(s) 1195, 1458
BseMII CTCAG 2 cut(s) 191, 1879
BseNI ACTGG 5 cut(s) 1254, 1338, 1541, 2078, 2126
BseRI GAGGAG 6 cut(s) 302, 457, 1015, 1535, 1569, 1839
BseXI GCAGC 6 cut(s) 244, 508, 942, 1058, 1732, 1877
BsgI GTGCAG 2 cut(s) 1065, 1173
BshFI GGCC 2 cut(s) 1447, 2167
BshNI GGYRCC 2 cut(s) 592, 1208
BsiHKAI GWGCWC 2 cut(s) 399, 502
BsiHKCI CYCGRG 2 cut(s) 383, 1657
BsiSI CCGG 6 cut(s) 56, 171, 212, 896, 1325, 2082
BslFI GGGAC 1 cut(s) 1344
BslI CCNNNNNNNGG 2 cut(s) 1454, 2073
BsmAI GTCTC 2 cut(s) 1660, 1779
BsmFI GGGAC 1 cut(s) 1344
BsmI GAATGC 1 cut(s) 1573
BsnI GGCC 2 cut(s) 1447, 2167
BsoBI CYCGRG 2 cut(s) 383, 1657
Bsp119I TTCGAA 1 cut(s) 1759
Bsp1286I GDGCHC 5 cut(s) 27, 226, 399, 502, 2198
Bsp1407I TGTACA 2 cut(s) 1140, 1734
Bsp143I GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
BspACI CCGC 2 cut(s) 209, 995
BspANI GGCC 2 cut(s) 1447, 2167
BspCNI CTCAG 2 cut(s) 192, 1878
BspLI GGNNCC 7 cut(s) 24, 419, 594, 1210, 1323, 1689, 2037
BspMAI CTGCAG 2 cut(s) 1594, 1895
BspPI GGATC 4 cut(s) 1116, 1397, 2030, 2043
BspQI GCTCTTC 1 cut(s) 972
BspT104I TTCGAA 1 cut(s) 1759
BspT107I GGYRCC 2 cut(s) 592, 1208
BsrDI GCAATG 2 cut(s) 1195, 1458
BsrFI RCCGGY 1 cut(s) 170
BsrGI TGTACA 2 cut(s) 1140, 1734
BsrI ACTGG 5 cut(s) 1254, 1338, 1541, 2078, 2126
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 384, 894, 1325
BssMI GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
BssNAI GTATAC 1 cut(s) 1534
BssNI GRCGYC 2 cut(s) 593, 1209
BssSI CACGAG 1 cut(s) 557
Bst1107I GTATAC 1 cut(s) 1534
Bst2BI CACGAG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 1206
Bst4CI ACNGT 7 cut(s) 71, 734, 839, 853, 1105, 1625, 2240
Bst6I CTCTTC 4 cut(s) 257, 972, 1436, 2238
BstACI GRCGYC 2 cut(s) 593, 1209
BstAUI TGTACA 2 cut(s) 1140, 1734
BstBAI YACGTR 2 cut(s) 678, 1462
BstBI TTCGAA 1 cut(s) 1759
BstC8I GCNNGC 3 cut(s) 1449, 1594, 1895
BstDEI CTNAG 6 cut(s) 200, 821, 917, 1057, 1865, 2098
BstEII GGTNACC 1 cut(s) 2077
BstF5I GGATG 4 cut(s) 220, 904, 1305, 2130
BstH2I RGCGCY 3 cut(s) 392, 596, 1212
BstHHI GCGC 5 cut(s) 391, 595, 904, 1027, 1211
BstKTI GATC 9 cut(s) 343, 790, 1124, 1258, 1405, 1552, 1711, 2038, 2220
BstMAI GTCTC 2 cut(s) 1660, 1779
BstMBI GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
BstMWI GCNNNNNNNGC 3 cut(s) 1215, 1778, 2164
BstNI CCWGG 1 cut(s) 1206
BstNSI RCATGY 1 cut(s) 2095
BstPI GGTNACC 1 cut(s) 2077
BstSCI CCNGG 5 cut(s) 54, 894, 1204, 1324, 2081
BstSFI CTRYAG 2 cut(s) 1590, 1891
BstSNI TACGTA 1 cut(s) 678
BstV1I GCAGC 6 cut(s) 244, 508, 942, 1058, 1732, 1877
BstV2I GAAGAC 2 cut(s) 2076, 2265
BstX2I RGATCY 4 cut(s) 1121, 1402, 2035, 2217
BstYI RGATCY 4 cut(s) 1121, 1402, 2035, 2217
BstZ17I GTATAC 1 cut(s) 1534
BsuI GTATCC 1 cut(s) 1451
BsuRI GGCC 2 cut(s) 1447, 2167
BtsCI GGATG 4 cut(s) 220, 904, 1305, 2130
BtsI GCAGTG 3 cut(s) 612, 1161, 1264
BtsIMutI CAGTG 6 cut(s) 67, 612, 797, 1161, 1264, 1966
Cac8I GCNNGC 3 cut(s) 1449, 1594, 1895
CfoI GCGC 5 cut(s) 391, 595, 904, 1027, 1211
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 3 cut(s) 1345, 1371, 2165
CseI GACGC 2 cut(s) 177, 1937
Csp6I GTAC 7 cut(s) 679, 835, 1141, 1271, 1525, 1662, 1735
CviAII CATG 9 cut(s) 60, 317, 683, 1041, 1279, 1377, 1528, 2062, 2092
CviQI GTAC 7 cut(s) 679, 835, 1141, 1271, 1525, 1662, 1735
DdeI CTNAG 6 cut(s) 200, 821, 917, 1057, 1865, 2098
DinI GGCGCC 2 cut(s) 594, 1210
DpnI GATC 9 cut(s) 342, 789, 1123, 1257, 1404, 1551, 1710, 2037, 2219
DpnII GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
Eam1104I CTCTTC 4 cut(s) 257, 972, 1436, 2238
EarI CTCTTC 4 cut(s) 257, 972, 1436, 2238
EciI GGCGGA 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 678
Eco147I AGGCCT 1 cut(s) 1447
Eco24I GRGCYC 3 cut(s) 27, 226, 2198
Eco47I GGWCC 2 cut(s) 1345, 1371
Eco57I CTGAAG 5 cut(s) 125, 278, 633, 864, 2206
Eco88I CYCGRG 2 cut(s) 383, 1657
Eco91I GGTNACC 1 cut(s) 2077
EcoO65I GGTNACC 1 cut(s) 2077
EcoRI GAATTC 2 cut(s) 709, 1860
EcoRII CCWGG 1 cut(s) 1204
EcoT22I ATGCAT 2 cut(s) 877, 1382
EcoT38I GRGCYC 3 cut(s) 27, 226, 2198
EgeI GGCGCC 2 cut(s) 594, 1210
EheI GGCGCC 2 cut(s) 594, 1210
FaeI CATG 9 cut(s) 63, 320, 686, 1044, 1282, 1380, 1531, 2065, 2095
FalI AAGNNNNNCTT 2 cut(s) 1165, 1197
FaqI GGGAC 1 cut(s) 1344
FatI CATG 9 cut(s) 59, 316, 682, 1040, 1278, 1376, 1527, 2061, 2091
FauI CCCGC 1 cut(s) 988
FauNDI CATATG 1 cut(s) 1819
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 3 cut(s) 191, 1178, 1533
Fnu4HI GCNGC 6 cut(s) 233, 522, 956, 1047, 1746, 1891
FokI GGATG 4 cut(s) 227, 911, 1312, 2137
FriOI GRGCYC 3 cut(s) 27, 226, 2198
Fsp4HI GCNGC 6 cut(s) 233, 522, 956, 1047, 1746, 1891
FspBI CTAG 4 cut(s) 242, 969, 1782, 2162
GlaI GCGC 5 cut(s) 390, 594, 903, 1026, 1210
GluI GCNGC 6 cut(s) 233, 522, 956, 1047, 1746, 1891
GsuI CTGGAG 3 cut(s) 582, 1355, 1770
HaeII RGCGCY 3 cut(s) 392, 596, 1212
HaeIII GGCC 2 cut(s) 1447, 2167
HapII CCGG 6 cut(s) 56, 171, 212, 896, 1325, 2082
HgaI GACGC 2 cut(s) 177, 1937
HhaI GCGC 5 cut(s) 391, 595, 904, 1027, 1211
Hin1I GRCGYC 2 cut(s) 593, 1209
Hin1II CATG 9 cut(s) 63, 320, 686, 1044, 1282, 1380, 1531, 2065, 2095
Hin6I GCGC 5 cut(s) 389, 593, 902, 1025, 1209
HinP1I GCGC 5 cut(s) 389, 593, 902, 1025, 1209
HincII GTYRAC 4 cut(s) 192, 1008, 1815, 1952
HindII GTYRAC 4 cut(s) 192, 1008, 1815, 1952
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 8 cut(s) 145, 238, 803, 863, 1036, 1304, 1906, 2135
HpaII CCGG 6 cut(s) 56, 171, 212, 896, 1325, 2082
HphI GGTGA 4 cut(s) 303, 332, 553, 2252
Hpy188I TCNGA 9 cut(s) 177, 201, 345, 880, 1172, 1398, 1859, 2021, 2119
Hpy188III TCNNGA 7 cut(s) 242, 383, 557, 599, 842, 1384, 1691
Hpy99I CGWCG 1 cut(s) 193
HpyAV CCTTC 8 cut(s) 172, 419, 450, 564, 839, 1167, 1755, 2080
HpyCH4III ACNGT 7 cut(s) 71, 734, 839, 853, 1105, 1625, 2240
HpyCH4IV ACGT 6 cut(s) 38, 677, 755, 813, 1461, 1954
HpyF10VI GCNNNNNNNGC 3 cut(s) 1215, 1778, 2164
HpyF3I CTNAG 6 cut(s) 200, 821, 917, 1057, 1865, 2098
HpySE526I ACGT 6 cut(s) 38, 677, 755, 813, 1461, 1954
Hsp92I GRCGYC 2 cut(s) 593, 1209
Hsp92II CATG 9 cut(s) 63, 320, 686, 1044, 1282, 1380, 1531, 2065, 2095
HspAI GCGC 5 cut(s) 389, 593, 902, 1025, 1209
KasI GGCGCC 2 cut(s) 592, 1208
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 6 cut(s) 244, 508, 942, 1058, 1732, 1877
LweI GCATC 8 cut(s) 592, 628, 862, 884, 889, 1389, 1683, 2170
MaeI CTAG 4 cut(s) 242, 969, 1782, 2162
MaeII ACGT 6 cut(s) 38, 677, 755, 813, 1461, 1954
MaeIII GTNAC 6 cut(s) 39, 119, 809, 1282, 1961, 2077
MalI GATC 9 cut(s) 342, 789, 1123, 1257, 1404, 1551, 1710, 2037, 2219
MboI GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
MfeI CAATTG 2 cut(s) 564, 642
MflI RGATCY 4 cut(s) 1121, 1402, 2035, 2217
MhlI GDGCHC 5 cut(s) 27, 226, 399, 502, 2198
Mly113I GGCGCC 2 cut(s) 593, 1209
MlyI GAGTC 4 cut(s) 247, 812, 1030, 1298
MmeI TCCRAC 5 cut(s) 649, 677, 919, 1323, 2142
Mph1103I ATGCAT 2 cut(s) 877, 1382
MslI CAYNNNNRTG 1 cut(s) 2060
MspA1I CMGCKG 2 cut(s) 103, 1567
MspI CCGG 6 cut(s) 56, 171, 212, 896, 1325, 2082
MspR9I CCNGG 5 cut(s) 56, 896, 1206, 1326, 2083
MunI CAATTG 2 cut(s) 564, 642
Mva1269I GAATGC 1 cut(s) 1573
MvaI CCWGG 1 cut(s) 1206
MwoI GCNNNNNNNGC 3 cut(s) 1215, 1778, 2164
NarI GGCGCC 2 cut(s) 593, 1209
NciI CCSGG 4 cut(s) 56, 896, 1326, 2083
NdeI CATATG 1 cut(s) 1819
NdeII GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
NlaIII CATG 9 cut(s) 63, 320, 686, 1044, 1282, 1380, 1531, 2065, 2095
NlaIV GGNNCC 7 cut(s) 24, 419, 594, 1210, 1323, 1689, 2037
NmuCI GTSAC 2 cut(s) 39, 1961
NsiI ATGCAT 2 cut(s) 877, 1382
NspI RCATGY 1 cut(s) 2095
NspV TTCGAA 1 cut(s) 1759
PceI AGGCCT 1 cut(s) 1447
PciI ACATGT 1 cut(s) 2091
PciSI GCTCTTC 1 cut(s) 972
PctI GAATGC 1 cut(s) 1573
PfeI GAWTC 4 cut(s) 145, 863, 1906, 2135
PflFI GACNNNGTC 1 cut(s) 1967
PkrI GCNGC 6 cut(s) 234, 523, 957, 1048, 1747, 1892
PleI GAGTC 4 cut(s) 246, 811, 1030, 1298
PluTI GGCGCC 2 cut(s) 596, 1212
PpsI GAGTC 4 cut(s) 246, 811, 1030, 1298
Ppu21I YACGTR 2 cut(s) 678, 1462
PscI ACATGT 1 cut(s) 2091
PshBI ATTAAT 1 cut(s) 303
PsiI TTATAA 2 cut(s) 887, 1983
Psp1406I AACGTT 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 1204
PspEI GGTNACC 1 cut(s) 2077
PspGI CCWGG 1 cut(s) 1204
PspN4I GGNNCC 7 cut(s) 24, 419, 594, 1210, 1323, 1689, 2037
PspPI GGNCC 3 cut(s) 1345, 1371, 2165
PstI CTGCAG 2 cut(s) 1594, 1895
PsuI RGATCY 4 cut(s) 1121, 1402, 2035, 2217
PsyI GACNNNGTC 1 cut(s) 1967
PvuII CAGCTG 2 cut(s) 103, 1567
RsaI GTAC 7 cut(s) 680, 836, 1142, 1272, 1526, 1663, 1736
RsaNI GTAC 7 cut(s) 679, 835, 1141, 1271, 1525, 1662, 1735
RseI CAYNNNNRTG 1 cut(s) 2060
SalI GTCGAC 1 cut(s) 190
SapI GCTCTTC 1 cut(s) 972
SatI GCNGC 6 cut(s) 233, 522, 956, 1047, 1746, 1891
Sau3AI GATC 9 cut(s) 340, 787, 1121, 1255, 1402, 1549, 1708, 2035, 2217
Sau96I GGNCC 3 cut(s) 1345, 1371, 2165
SchI GAGTC 4 cut(s) 247, 812, 1030, 1298
ScrFI CCNGG 5 cut(s) 56, 896, 1206, 1326, 2083
SduI GDGCHC 5 cut(s) 27, 226, 399, 502, 2198
SfaNI GCATC 8 cut(s) 592, 628, 862, 884, 889, 1389, 1683, 2170
SfcI CTRYAG 2 cut(s) 1590, 1891
SfoI GGCGCC 2 cut(s) 594, 1210
SfuI TTCGAA 1 cut(s) 1759
SinI GGWCC 2 cut(s) 1345, 1371
SmiMI CAYNNNNRTG 1 cut(s) 2060
SmlI CTYRAG 2 cut(s) 392, 1943
SmoI CTYRAG 2 cut(s) 392, 1943
SnaBI TACGTA 1 cut(s) 678
SpeI ACTAGT 1 cut(s) 968
SseBI AGGCCT 1 cut(s) 1447
SsiI CCGC 2 cut(s) 209, 995
SspDI GGCGCC 2 cut(s) 592, 1208
SspI AATATT 2 cut(s) 1087, 1617
SspMI CTAG 4 cut(s) 242, 969, 1782, 2162
StuI AGGCCT 1 cut(s) 1447
StyD4I CCNGG 5 cut(s) 54, 894, 1204, 1324, 2081
TaaI ACNGT 7 cut(s) 71, 734, 839, 853, 1105, 1625, 2240
TaiI ACGT 6 cut(s) 41, 680, 758, 816, 1464, 1957
TaqI TCGA 7 cut(s) 191, 786, 1071, 1677, 1759, 1883, 2133
TaqII GACCGA 1 cut(s) 2081
TatI WGTACW 4 cut(s) 834, 1140, 1524, 1734
TfiI GAWTC 4 cut(s) 145, 863, 1906, 2135
TscAI CASTG 6 cut(s) 74, 612, 797, 1161, 1271, 1966
TseFI GTSAC 2 cut(s) 39, 1961
TseI GCWGC 6 cut(s) 232, 521, 955, 1046, 1745, 1890
Tsp45I GTSAC 2 cut(s) 39, 1961
TspDTI ATGAA 2 cut(s) 137, 1382
TspRI CASTG 6 cut(s) 74, 612, 797, 1161, 1271, 1966
Tth111I GACNNNGTC 1 cut(s) 1967
VpaK11BI GGWCC 2 cut(s) 1345, 1371
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 5 cut(s) 709, 889, 1729, 1837, 1860
XbaI TCTAGA 1 cut(s) 241
XceI RCATGY 1 cut(s) 2095
XmiI GTMKAC 3 cut(s) 191, 1178, 1533
XspI CTAG 4 cut(s) 242, 969, 1782, 2162
Zsp2I ATGCAT 2 cut(s) 877, 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.