MD02G1265900.v1.1

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000444
Physical Location & Seq
Forward (+)
31993324 .. 31999677
6354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1265900.v1.1.491

Sequence Viewer

Length: 2223 bp
ATGCAGCACAAGGATAATCAAGGTTCAGTTCCACCTCGTGCTTCTGATCCTGGCTGGGCCCATGGCATTATGGTGAATGGAGGTCGTCAGAAGATTAAGTGCAAATACTGCCACAAAGTAATGCTTGGGGGTGGAATTTCCAGACTAAAACAGCATCTGGCTGGGGAAAGGGGGAATGTAGCTCCATGCGAGGAGGTACCTGAAGAAGTTAAAATGAAGATTCAACAACACCTAGGTTTTAAAGTACTTGAGAAGCTTAAAAAGCAGAAAGGATTAAACACTAGCAGGGATTCCGCATCCTACCAGTATAGGGAAGAAGAAGACAATGAAGATGGAATGAGAACTCAAAAGAAAGTTTCCACACGAGGCACGCGAAGGAGAAGGGGAAAAGAGGCTACAGAAGGAATTTCCAATAAGAAACACAAAAAGCATTCTTTTCCTGCTGCAACAGCTAATGTTGCTCAGCCTTTGCATCAGAGTTTTGCTTCTCAAGATATGATAGATCAAGCTGATTTGGCTGTTGCAAGATTTATGTATGAATCTGGTATACCATTCACTGCTGCAAATTCGCATTTTTATCAACAGATGGCTGATGCAATTGCTGCTGTTGGTCCTGGCTATAAGATGCCTTCTTATAATGCTTTGGGGGGAAAATTGTTGAACCACAGCGTTCAGGATGCAGAAGAATATGGAGAGGAACTGAGAAGATCTTGGGATGTGACTGGGTGTTCAGTCATGGTTGATAGGTGGGTGGATAAAGGTGGTCATACAGTTATCAACTTCTTTGTATACTGCCCCAAGGGCACTGTGTTCCTCAAATCTGCAGATGCGTCAGACATCTCAGAATGTCCAGAAGCACTTCTTAATTTATTTGACGGTGTTGTTCAAGAAGTTGGGCCTCAGAGGATTGTCAATTTTGTGACAGATGCTTCTCCCAGTTGTAAAGCTGCTAGAAAGCTTTTAATGGAGAAATATAAGACCTTTTTCTGCAGTACCTGCGGAGCACATGGTGTTGACTTAATGCTTGAGGAAATTGGGAAAATGAATGAAGTTAAAGAGGTCTTGGCAAAGGCAAAAAGAGTAACTCGATTTGTATACAACAGTGCTTGGATGCTTAATCTGGTAAGGAAGAAAACAGGTGGAAGAGAGATTGTCCAGCTTGCAACCACAAGGTTTGCTTCTGTCTTCCTTACGCTGCAGAATATTGTAGCTCTGAAGCACCAGCTCCAACAAATATTTACCACTGCTGCTTGGATGCAGTCAAATTTCTCTAAGCAAAGGGCTGGACTAGAGGTAGCAGAGATTGTTGCAGACCAACTTTTCTGGTCACTGTGTGATCAAACTCTGAAGGTGACAAAGCACCTACTCTCGGTTTTTCATCTTATGGATTGTGAGGAGAAACCATCTATTGGTTACTTATACGATGCAATTGAAAAGGCAAAGAAAAGTGTAATTGTGGCATTTGACAATAATGAACCTGATTACTTGCCATATTTGAAGGTTATTGATCACGTTTGGCAAGAGGAGCTCCATAGCCCACTTCATGCAGCTGCATGCTATCTCAACCCGTCTATATTTTATAGCCCTACCTTTTCTACTAAAAAAGTCATCCAGAAGGGATTGCTTGACTGTATTGAAACCATAGAGCCTAACTTAACAGCTCAAGTTGTGATTACAAGCAACATAAATTTCTATGAGGAAGCTGTAGGGGATTTTGGTCGACCCGTGGCTTTACGATGTCGAGAATCATTGGCCCCAGCTACTTGGTGGTCCCTGTATGGAGCTGATTACCCTGATCTCCAACGGTTAGCTGTCAGGATACTAAGTCAAACTTGCAGCATTATCCGGTCTAAGAGGAGTGGGAGCATATTTGAACGTATGTATTCAAAGAAAAGGAACCGATTGGAGCAACAGAGGTTGAACGACCTTGTATTTGTTCATCATAACTTGCACCTTCAACATATGCGTTCTGAAGCAAGTAAAGGCGTATATACAAGAGGTACCCTGGATCCAATATGTTTAGAAGCTATGGATTCCAACATTGAAGATTGGGTGGAAGACCTGGGAGATACTGGCAGCGATGAGTTAAGGTGGATGGATGTGATGGTCCCTGGTGAGTGGAAAGTTTTGAATCATAGAGTACAGAGTACCAACGATTGTAATGACAGTACAGATGACAGAGGTAGTGATGATGGGAGAGGTATGGACATAAATGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

741

Amino Acids

83.45

Weight (kDa)

7.3

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 210 - 361 5.4e-54 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 583 - 650 3.8e-14 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
Acc36I ACCTGC 1 cut(s) 1004
Acc65I GGTACC 2 cut(s) 196, 2000
AccB1I GGYRCC 2 cut(s) 196, 2000
AccB7I CCANNNNNTGG 1 cut(s) 1409
AccI GTMKAC 4 cut(s) 547, 789, 1095, 1720
AccII CGCG 1 cut(s) 373
AciI CCGC 2 cut(s) 294, 999
AclWI GGATC 3 cut(s) 41, 2003, 2016
AcsI RAATTY 5 cut(s) 135, 405, 565, 1264, 1687
AcuI CTGAAG 4 cut(s) 222, 1235, 1367, 1992
AdeI CACNNNGTG 3 cut(s) 38, 1010, 1334
AfaI GTAC 7 cut(s) 198, 246, 994, 2002, 2142, 2149, 2170
AfiI CCNNNNNNNGG 3 cut(s) 310, 1369, 1409
AjnI CCWGG 5 cut(s) 49, 613, 2004, 2061, 2110
Alw21I GWGCWC 2 cut(s) 1006, 1530
AlwI GGATC 3 cut(s) 41, 2003, 2016
AlwNI CAGNNNCTG 2 cut(s) 157, 996
AoxI GGCC 3 cut(s) 57, 896, 1752
ApaI GGGCCC 1 cut(s) 61
ApoI RAATTY 5 cut(s) 135, 405, 565, 1264, 1687
ArsI GACNNNNNNTTYG 2 cut(s) 1916, 1948
Asp700I GAANNNNTTC 1 cut(s) 858
Asp718I GGTACC 2 cut(s) 196, 2000
AspA2I CCTAGG 1 cut(s) 232
AspS9I GGNCC 7 cut(s) 57, 58, 611, 896, 1753, 1770, 2107
AsuHPI GGTGA 3 cut(s) 85, 1363, 2126
AvaII GGWCC 3 cut(s) 611, 1770, 2107
AvrII CCTAGG 1 cut(s) 232
BaeGI GKGCMC 2 cut(s) 61, 806
BamHI GGATCC 1 cut(s) 2008
BanI GGYRCC 2 cut(s) 196, 2000
BanII GRGCYC 2 cut(s) 61, 1530
BauI CACGAG 2 cut(s) 36, 363
BbsI GAAGAC 3 cut(s) 327, 1177, 2064
Bbv12I GWGCWC 2 cut(s) 1006, 1530
BccI CCATC 6 cut(s) 326, 580, 1411, 2089, 2098, 2186
BciT130I CCWGG 5 cut(s) 51, 615, 2006, 2063, 2112
BciVI GTATCC 1 cut(s) 1812
BclI TGATCA 2 cut(s) 1336, 1507
BfaI CTAG 4 cut(s) 233, 282, 951, 1289
BfmI CTRYAG 5 cut(s) 396, 822, 988, 1196, 1704
BfuAI ACCTGC 1 cut(s) 1004
BfuI GTATCC 1 cut(s) 1812
BglI GCCNNNNNGGC 1 cut(s) 801
BglII AGATCT 1 cut(s) 707
BlnI CCTAGG 1 cut(s) 232
BlpI GCTNAGC 1 cut(s) 462
BmcAI AGTACT 1 cut(s) 246
Bme1390I CCNGG 5 cut(s) 51, 615, 2006, 2063, 2112
Bme18I GGWCC 3 cut(s) 611, 1770, 2107
BmgT120I GGNCC 7 cut(s) 57, 58, 611, 896, 1753, 1770, 2107
BmiI GGNNCC 8 cut(s) 59, 198, 1755, 1772, 1898, 2002, 2010, 2109
BmrFI CCNGG 5 cut(s) 51, 615, 2006, 2063, 2112
BmrI ACTGGG 2 cut(s) 732, 930
BmuI ACTGGG 2 cut(s) 732, 930
BpiI GAAGAC 3 cut(s) 327, 1177, 2064
Bpu1102I GCTNAGC 1 cut(s) 462
BpuEI CTTGAG 4 cut(s) 269, 474, 1046, 1647
BsaJI CCNNGG 7 cut(s) 61, 232, 798, 1725, 2004, 2062, 2110
BsaWI WCCGGW 1 cut(s) 1845
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 1369, 1409
Bse1I ACTGG 4 cut(s) 304, 727, 936, 2077
BseBI CCWGG 5 cut(s) 51, 615, 2006, 2063, 2112
BseDI CCNNGG 7 cut(s) 61, 232, 798, 1725, 2004, 2062, 2110
BseGI GGATG 8 cut(s) 296, 682, 721, 1116, 1260, 1608, 2100, 2104
BseLI CCNNNNNNNGG 3 cut(s) 310, 1369, 1409
BseMII CTCAG 4 cut(s) 476, 692, 855, 914
BseNI ACTGG 4 cut(s) 304, 727, 936, 2077
BseRI GAGGAG 4 cut(s) 206, 1409, 1538, 1870
BseSI GKGCMC 2 cut(s) 61, 806
BseYI CCCAGC 3 cut(s) 54, 161, 1756
Bsh1236I CGCG 1 cut(s) 373
BshFI GGCC 3 cut(s) 59, 898, 1754
BshNI GGYRCC 2 cut(s) 196, 2000
BsiHKAI GWGCWC 2 cut(s) 1006, 1530
BsiSI CCGG 1 cut(s) 1846
BslFI GGGAC 2 cut(s) 1756, 2093
BslI CCNNNNNNNGG 3 cut(s) 310, 1369, 1409
BsmFI GGGAC 2 cut(s) 1756, 2093
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 3 cut(s) 59, 898, 1754
Bsp120I GGGCCC 1 cut(s) 57
Bsp1286I GDGCHC 4 cut(s) 61, 806, 1006, 1530
Bsp143I GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
Bsp1720I GCTNAGC 1 cut(s) 462
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 2 cut(s) 294, 999
BspANI GGCC 3 cut(s) 59, 898, 1754
BspCNI CTCAG 4 cut(s) 475, 693, 854, 913
BspFNI CGCG 1 cut(s) 373
BspLI GGNNCC 8 cut(s) 59, 198, 1755, 1772, 1898, 2002, 2010, 2109
BspMAI CTGCAG 3 cut(s) 826, 992, 1200
BspMI ACCTGC 1 cut(s) 1004
BspPI GGATC 3 cut(s) 41, 2003, 2016
BspT107I GGYRCC 2 cut(s) 196, 2000
BsrI ACTGG 4 cut(s) 304, 727, 936, 2077
BssECI CCNNGG 7 cut(s) 61, 232, 798, 1725, 2004, 2062, 2110
BssMI GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
BssNAI GTATAC 3 cut(s) 548, 790, 1096
BssSI CACGAG 2 cut(s) 36, 363
BssT1I CCWWGG 3 cut(s) 61, 232, 798
Bst1107I GTATAC 3 cut(s) 548, 790, 1096
Bst2BI CACGAG 2 cut(s) 36, 363
Bst2UI CCWGG 5 cut(s) 51, 615, 2006, 2063, 2112
Bst4CI ACNGT 8 cut(s) 772, 808, 878, 1103, 1332, 1631, 1806, 2168
Bst6I CTCTTC 1 cut(s) 1138
BstAPI GCANNNNNTGC 3 cut(s) 108, 602, 996
BstC8I GCNNGC 3 cut(s) 371, 1161, 1555
BstDEI CTNAG 7 cut(s) 462, 701, 841, 900, 1272, 1823, 1851
BstDSI CCRYGG 2 cut(s) 61, 1725
BstF5I GGATG 8 cut(s) 296, 682, 721, 1116, 1260, 1608, 2100, 2104
BstFNI CGCG 1 cut(s) 373
BstKTI GATC 7 cut(s) 49, 505, 710, 1339, 1510, 1798, 2011
BstMBI GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
BstMWI GCNNNNNNNGC 9 cut(s) 108, 262, 449, 458, 515, 602, 801, 996, 1525
BstNI CCWGG 5 cut(s) 51, 615, 2006, 2063, 2112
BstNSI RCATGY 1 cut(s) 1557
BstSCI CCNGG 5 cut(s) 49, 613, 2004, 2061, 2110
BstSFI CTRYAG 5 cut(s) 396, 822, 988, 1196, 1704
BstSLI GKGCMC 2 cut(s) 61, 806
BstUI CGCG 1 cut(s) 373
BstV2I GAAGAC 3 cut(s) 327, 1177, 2064
BstX2I RGATCY 2 cut(s) 707, 2008
BstXI CCANNNNNNTGG 1 cut(s) 1764
BstYI RGATCY 2 cut(s) 707, 2008
BstZ17I GTATAC 3 cut(s) 548, 790, 1096
BsuI GTATCC 1 cut(s) 1812
BsuRI GGCC 3 cut(s) 59, 898, 1754
BtgI CCRYGG 2 cut(s) 61, 1725
BtgZI GCGATG 1 cut(s) 2094
BtsCI GGATG 8 cut(s) 296, 682, 721, 1116, 1260, 1608, 2100, 2104
BtsI GCAGTG 2 cut(s) 555, 1242
BtsIMutI CAGTG 5 cut(s) 555, 804, 1108, 1242, 1328
BveI ACCTGC 1 cut(s) 1004
Cac8I GCNNGC 3 cut(s) 371, 1161, 1555
CaiI CAGNNNCTG 2 cut(s) 157, 996
Cfr13I GGNCC 7 cut(s) 57, 58, 611, 896, 1753, 1770, 2107
CseI GACGC 1 cut(s) 819
Csp6I GTAC 7 cut(s) 197, 245, 993, 2001, 2141, 2148, 2169
CspCI CAANNNNNGTGG 2 cut(s) 1156, 1191
CviAII CATG 6 cut(s) 62, 186, 736, 1007, 1544, 1554
CviQI GTAC 7 cut(s) 197, 245, 993, 2001, 2141, 2148, 2169
DdeI CTNAG 7 cut(s) 462, 701, 841, 900, 1272, 1823, 1851
DpnI GATC 7 cut(s) 48, 504, 709, 1338, 1509, 1797, 2010
DpnII GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
DraI TTTAAA 1 cut(s) 241
DraIII CACNNNGTG 3 cut(s) 38, 1010, 1334
Eam1104I CTCTTC 1 cut(s) 1138
EarI CTCTTC 1 cut(s) 1138
Ecl136II GAGCTC 1 cut(s) 1528
Eco130I CCWWGG 3 cut(s) 61, 232, 798
Eco24I GRGCYC 2 cut(s) 61, 1530
Eco47I GGWCC 3 cut(s) 611, 1770, 2107
Eco53kI GAGCTC 1 cut(s) 1528
Eco57I CTGAAG 4 cut(s) 222, 1235, 1367, 1992
EcoICRI GAGCTC 1 cut(s) 1528
EcoRII CCWGG 5 cut(s) 49, 613, 2004, 2061, 2110
EcoT14I CCWWGG 3 cut(s) 61, 232, 798
EcoT38I GRGCYC 2 cut(s) 61, 1530
ErhI CCWWGG 3 cut(s) 61, 232, 798
FaeI CATG 6 cut(s) 65, 189, 739, 1010, 1547, 1557
FalI AAGNNNNNCTT 8 cut(s) 108, 140, 846, 878, 1162, 1194, 1816, 1848
FaqI GGGAC 2 cut(s) 1756, 2093
FatI CATG 6 cut(s) 61, 185, 735, 1006, 1543, 1553
FauNDI CATATG 1 cut(s) 1962
FbaI TGATCA 2 cut(s) 1336, 1507
FblI GTMKAC 4 cut(s) 547, 789, 1095, 1720
FokI GGATG 8 cut(s) 283, 689, 728, 1123, 1267, 1595, 2107, 2111
FriOI GRGCYC 2 cut(s) 61, 1530
FspBI CTAG 4 cut(s) 233, 282, 951, 1289
GsaI CCCAGC 3 cut(s) 58, 165, 1760
HaeIII GGCC 3 cut(s) 59, 898, 1754
HapII CCGG 1 cut(s) 1846
HgaI GACGC 1 cut(s) 819
Hin1II CATG 6 cut(s) 65, 189, 739, 1010, 1547, 1557
HincII GTYRAC 2 cut(s) 1015, 1721
HindII GTYRAC 2 cut(s) 1015, 1721
HindIII AAGCTT 2 cut(s) 254, 956
HinfI GANTC 6 cut(s) 220, 290, 539, 1745, 2033, 2131
HpaII CCGG 1 cut(s) 1846
HphI GGTGA 3 cut(s) 85, 1363, 2126
Hpy166II GTNNAC 5 cut(s) 548, 790, 1015, 1096, 1721
Hpy188I TCNGA 9 cut(s) 46, 90, 477, 835, 844, 903, 1215, 1347, 1972
Hpy188III TCNNGA 8 cut(s) 141, 491, 674, 851, 887, 1612, 1742, 1816
Hpy8I GTNNAC 5 cut(s) 548, 790, 1015, 1096, 1721
HpyAV CCTTC 8 cut(s) 369, 375, 395, 639, 1342, 1492, 1609, 1964
HpyCH4III ACNGT 8 cut(s) 772, 808, 878, 1103, 1332, 1631, 1806, 2168
HpyCH4IV ACGT 2 cut(s) 1512, 1876
HpyF10VI GCNNNNNNNGC 9 cut(s) 108, 262, 449, 458, 515, 602, 801, 996, 1525
HpyF3I CTNAG 7 cut(s) 462, 701, 841, 900, 1272, 1823, 1851
HpySE526I ACGT 2 cut(s) 1512, 1876
Hsp92II CATG 6 cut(s) 65, 189, 739, 1010, 1547, 1557
KpnI GGTACC 2 cut(s) 200, 2004
Ksp22I TGATCA 2 cut(s) 1336, 1507
Kzo9I GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
LmnI GCTCC 8 cut(s) 187, 1001, 1230, 1525, 1533, 1781, 1863, 1906
MaeI CTAG 4 cut(s) 233, 282, 951, 1289
MaeII ACGT 2 cut(s) 1512, 1876
MaeIII GTNAC 6 cut(s) 718, 919, 1081, 1326, 1351, 1412
MalI GATC 7 cut(s) 48, 504, 709, 1338, 1509, 1797, 2010
MboI GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
MfeI CAATTG 2 cut(s) 597, 1428
MflI RGATCY 2 cut(s) 707, 2008
MhlI GDGCHC 4 cut(s) 61, 806, 1006, 1530
MmeI TCCRAC 3 cut(s) 1252, 1825, 2061
MroXI GAANNNNTTC 1 cut(s) 858
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 1550
MspI CCGG 1 cut(s) 1846
MspR9I CCNGG 5 cut(s) 51, 615, 2006, 2063, 2112
MunI CAATTG 2 cut(s) 597, 1428
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 5 cut(s) 51, 615, 2006, 2063, 2112
MvnI CGCG 1 cut(s) 373
MwoI GCNNNNNNNGC 9 cut(s) 108, 262, 449, 458, 515, 602, 801, 996, 1525
NcoI CCATGG 1 cut(s) 61
NdeI CATATG 1 cut(s) 1962
NdeII GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
NlaIII CATG 6 cut(s) 65, 189, 739, 1010, 1547, 1557
NlaIV GGNNCC 8 cut(s) 59, 198, 1755, 1772, 1898, 2002, 2010, 2109
NmuCI GTSAC 4 cut(s) 718, 919, 1326, 1351
NspI RCATGY 1 cut(s) 1557
PaeI GCATGC 1 cut(s) 1557
PcsI WCGNNNNNNNCGW 1 cut(s) 370
PctI GAATGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 858
PfeI GAWTC 6 cut(s) 220, 290, 539, 1745, 2033, 2131
PflMI CCANNNNNTGG 1 cut(s) 1409
PsiI TTATAA 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 1530
Psp6I CCWGG 5 cut(s) 49, 613, 2004, 2061, 2110
PspFI CCCAGC 3 cut(s) 54, 161, 1756
PspGI CCWGG 5 cut(s) 49, 613, 2004, 2061, 2110
PspN4I GGNNCC 8 cut(s) 59, 198, 1755, 1772, 1898, 2002, 2010, 2109
PspOMI GGGCCC 1 cut(s) 57
PspPI GGNCC 7 cut(s) 57, 58, 611, 896, 1753, 1770, 2107
PstI CTGCAG 3 cut(s) 826, 992, 1200
PstNI CAGNNNCTG 2 cut(s) 157, 996
PsuI RGATCY 2 cut(s) 707, 2008
PvuII CAGCTG 1 cut(s) 1550
RsaI GTAC 7 cut(s) 198, 246, 994, 2002, 2142, 2149, 2170
RsaNI GTAC 7 cut(s) 197, 245, 993, 2001, 2141, 2148, 2169
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 1530
SalI GTCGAC 1 cut(s) 1719
Sau3AI GATC 7 cut(s) 46, 502, 707, 1336, 1507, 1795, 2008
Sau96I GGNCC 7 cut(s) 57, 58, 611, 896, 1753, 1770, 2107
ScaI AGTACT 1 cut(s) 246
ScrFI CCNGG 5 cut(s) 51, 615, 2006, 2063, 2112
SduI GDGCHC 4 cut(s) 61, 806, 1006, 1530
SfcI CTRYAG 5 cut(s) 396, 822, 988, 1196, 1704
SinI GGWCC 3 cut(s) 611, 1770, 2107
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 4 cut(s) 248, 489, 1025, 1662
SmoI CTYRAG 4 cut(s) 248, 489, 1025, 1662
SphI GCATGC 1 cut(s) 1557
SsiI CCGC 2 cut(s) 294, 999
SspI AATATT 2 cut(s) 1204, 1236
SspMI CTAG 4 cut(s) 233, 282, 951, 1289
SstI GAGCTC 1 cut(s) 1530
StyD4I CCNGG 5 cut(s) 49, 613, 2004, 2061, 2110
StyI CCWWGG 3 cut(s) 61, 232, 798
TaaI ACNGT 8 cut(s) 772, 808, 878, 1103, 1332, 1631, 1806, 2168
TaiI ACGT 2 cut(s) 1515, 1879
TaqI TCGA 3 cut(s) 1087, 1720, 1741
TatI WGTACW 3 cut(s) 244, 2140, 2168
TfiI GAWTC 6 cut(s) 220, 290, 539, 1745, 2033, 2131
TscAI CASTG 5 cut(s) 562, 811, 1108, 1249, 1335
TseFI GTSAC 4 cut(s) 718, 919, 1326, 1351
Tsp45I GTSAC 4 cut(s) 718, 919, 1326, 1351
TspDTI ATGAA 9 cut(s) 230, 342, 552, 1058, 1062, 1367, 1488, 1532, 1928
TspRI CASTG 5 cut(s) 562, 811, 1108, 1249, 1335
Van91I CCANNNNNTGG 1 cut(s) 1409
VpaK11BI GGWCC 3 cut(s) 611, 1770, 2107
XapI RAATTY 5 cut(s) 135, 405, 565, 1264, 1687
XceI RCATGY 1 cut(s) 1557
XcmI CCANNNNNNNNNTGG 1 cut(s) 1764
XmaJI CCTAGG 1 cut(s) 232
XmiI GTMKAC 4 cut(s) 547, 789, 1095, 1720
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 4 cut(s) 233, 282, 951, 1289
ZrmI AGTACT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.