Rorug07G0297200

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000444
Physical Location & Seq
Forward (+)
29305570 .. 29312333
6764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0297200.1

Sequence Viewer

Length: 3231 bp
ATGGAAGAGGTTGGTGCCCAAGTTGCTGCCCCAATTTTCCTCCGGCAATCGCTTTCGAGTCGGTTCTGCGACGCGCCGGCGGCCATGGGTAAGAAGAAGCGCGATCTCCCTTACCAGACCCCAAACTATCAACACCCAAATTCGCAGCCGAGGTTCGCAAACCCCGGGACCAACAGCTGGAACCCAAACGTTTGGGGCTGGGACAGTATGAGGTTTGTGGCCAAGCCACTGGACACTGAGATGATGGGTACTTCCAATTCAGAGCCCAAGAGGAAGGAGGAAGCTGCTGCTGCTGCTGCTGCTGGAGTTGTGAAGAGCACCGCCGTGGTGGAGGATGATGATGAACGCCTTCAGCTGAATCTGGGTGGTGGGTTGGCTTCTGTGGAGGAGCCGGCGGCATCCAGGCCGAATAAGAGGGTCCGGTCGGGATCTCCGGGGAATAATGGTGGAAGCTACCCCATGTGCCAAGTTGATGATTGTAAAGAGGATCTGTCAAGTGCCAAAGACTATCATCGCCGTCATAAAGTCTGTGAGAGTCACAGTAAATCCACCAAAGCCCTAGTTGCTAAGCAGATGCAGAGGTTCTGCCAGCAGTGCAGCAGGTTTCACCCGCTTTCAGAGTTTGATGAGGGGAAGAGAAGTTGCAGAAGGAGACTTGCGGGGCATAACAGGCGAAGAAGGAAAACCCAGCCTGAGGATGTTACCTCAAGGCTCACTATCCCTGGAGATGGGGATAACAAAACTAGTGGAAATTTGGATATTGTCAACTTGTTAGCTGCTATAACTCGTCCACAGGGGAAAAATGATGTCCGAACTACCAACGGCTCGTCGGTACTGGACAGAGAGCAGCTCCTTCAGATTCTTAGTAAGATAAATTCATTGCCTTTGCCAGTAGACCTTGCTGCCAAGTTGCCGAATCTCGGAAATTTAAATTGGAAAGCTTCAGACCTACTTTCTTTAGATCTTCAAAACAAACTGAATGGAAAAACATCTGCGTCAACCATGGACTTGATTACTGTTCTTTCAGCTACTCTAGCAGCACCTTCTTCTGACACACTTGCTATGTTATCTCAAAAGAGCAGTCAGAGTAGTGACAGTGAGAAAACTAAGTTAACATGCTCTGACCAGGCAGCTGGTCCTAATTTGCAGAAGAGGCCTCCTCAAGAGTTTCATTCAGCTGGGGGAGAGAGAAGTAGTACTAGCTACCAGTCTCCTACAGAAGATTCAGATTGCCAGGTTCAAGAGACTCGAGTCAAATTACCTTTACAGCTATTCAGCTCCTCACCTGAGGATGACAGTCCACCAAAACTGGCTTCTTCCAGAAAGTATTTCTCTTCAGACAGTAGTAATCGCACAGAGGAGAGGTCTCCATCATCGTCACCTCCTGTAATGCAAACACTGTTTCCAATGAAGAGTATGGCAGACACTGTCAAGTCGGAGAAACTTTCAATAAGCAAAGATTTGAATGTGAACCTGGACTCTAGCATGAACGGTGGTAGTAACTTGCCTTTTGATCTTTTTAGAGGGCCAAATAGAGGAGCTGTCAGCTCCTCCATTCAAAATTTTCCACCCCAAGCTGGCTATACATCTTCTAGTTCTGATCATTCACCGTCTAGCTTGAATTCTGATCCTCAGGATCGTACTGGAAGAATACTGTTTAAACTATTTGATAAGGATCCGAGTCAGTTACCAGGGACATTGCGGACACAGGTATACAGTTGGCTTTCTAACAGTCCGTCTGAAATGGAGAGCTACATAAGGCCTGGTTGTGTGGTCCTATCAGTTTATGTGTCAATGTCATTTGCTGCCTGGGAGCATCTTGAAGAAAACCTGGTCCAACGTGTCAGTTCTTTAATACAAAGTTCAGATTCTGATTTTTGGAGAAGTGGAAGGTTTTTAGTAAATACAGGGAGGCAATTAGCTTCTCATAAAGATGGAAAGATTCGTCTATGCAAAGCCTGGACAACATTTAGTTCACCAGAGTTAATCTCTGTGTCCCCTTTGGCTGTCGTGGGCGGGCAAAAAACCTCTCTTTCAATAAGGGGTAGAAATCTGACTAATCTTGGCACCAAGATTCATTGCACGTATATGGGTGGTTACACATCAAAGGAAGTTGGAACAACATATCATGGAACCGAGTATGATGAGATAAATTTGGGAAGTTTTCAAATTCATGATGCATCTCCCGGTGTTCTAGGTCGTTGTTTCATAGAGGTGGAAAATGGTTTTAAGGGAAACAGCTTTCCTGTGATCATAGCTGATGCTACAATCTGTAGCGAATTGAATCTCATTGAGTCTGAGTTTGATGGGGAGACAAAGGTATGCAGTGCAATTTCAGAAGATGAAAATCATGATTATGGGCGGCCCAGGTCAAGGGAGGAAGTCCTTCACTTTCTGAACGAGCTAGGATGGCTCTTCCAAAGGAAAAAGATCTCTTCCATGTTTCAAGGGTCCGGCTACTCTCTTAGCCGTTTCAAATTTTTACTCACATTCACTGTTGAGAGAGACTGTTGTACTTTGGTGAAAACACTTCTAGACATATTGGTAAATTTCGATGGGAATGAGCTATCAAGAGAGTCATTGGGGATGCTGTCAGATGTCCAGCTGTTAAACCGGGCTGTAAAAAGGAGGTGCAGGAAGATGATTGACTTGCTTATTAATTATTCTGTGATTAGCAGTGACAAGAGATACATTTTTCCTCCAAATCTTGCTGGACCTGGTGGCCTGACACCTCTTCATTTGGCTGCTTCTTTGTCAAATTCGGAGGATATGATTGATGCTTTGACGAATGATCCACAGGAGATCGGATTGAACTGCTGGCATTCCCTTTTGGATGGTAATGGGCAGTCTCCATATGCTTATGCTATGATGAGGAATAATTACTCTTACAACAATCTGGTGGCACGCAAACTTACTGACAGAAGAAATAGTCAGGTTACCTTAACAATTGGAAATGAGATAGACCAAACCCATATGGCGATGGAGCTGGAGCGTAGGACAAGTATCCAATTCAGACAAGGTAGCAGATCATGTGCCAAATGCGCAGTTGCAGCAACAAAATACACTAGGAGGGTTCCTGGTGCTCAAGGCTTGCTTCAGCGTCCATTCATTCATTCTATGCTTGCCATAGCTGCTGTTTGTGTTTGTGTCTGCTTGTTCTTGAGAGGTTCCCCAGACATTGGCTCAGTTGCTCCTTTCAAGTGGGAGAATTTGGATTTTGGCACAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

1076

Amino Acids

118.64

Weight (kDa)

8.49

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 155 - 228 3.4e-30 SBP domain
Ig_SPL7 PF26102 658 - 762 8e-35 SPL7-like, immunoglobulin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3043
Acc36I ACCTGC 1 cut(s) 591
AccB1I GGYRCC 2 cut(s) 14, 2066
AccB7I CCANNNNNTGG 2 cut(s) 177, 3179
AccI GTMKAC 2 cut(s) 894, 1713
AccII CGCG 2 cut(s) 74, 102
AciI CCGC 8 cut(s) 80, 321, 395, 611, 659, 1702, 2016, 2362
AclI AACGTT 1 cut(s) 189
AclWI GGATC 7 cut(s) 436, 495, 1622, 1644, 1670, 1683, 2786
AcoI YGGCCR 2 cut(s) 81, 219
AcuI CTGAAG 5 cut(s) 335, 839, 927, 1320, 3080
AfaI GTAC 5 cut(s) 250, 834, 1198, 1642, 2515
AfiI CCNNNNNNNGG 8 cut(s) 177, 694, 728, 920, 1535, 1577, 2373, 3179
AflIII ACRYGT 1 cut(s) 1840
AhlI ACTAGT 1 cut(s) 743
AjuI GAANNNNNNNTTGG 4 cut(s) 916, 948, 1297, 1329
AleI CACNNNNGTG 1 cut(s) 323
Alw21I GWGCWC 2 cut(s) 320, 3085
Alw26I GTCTC 7 cut(s) 646, 1215, 1238, 1371, 2306, 2499, 2853
AlwI GGATC 7 cut(s) 436, 495, 1622, 1644, 1670, 1683, 2786
AlwNI CAGNNNCTG 2 cut(s) 1427, 1871
Ama87I CYCGRG 2 cut(s) 164, 1248
AoxI GGCC 8 cut(s) 81, 219, 404, 1154, 1526, 1760, 2363, 2722
ArsI GACNNNNNNTTYG 2 cut(s) 1066, 1098
AseI ATTAAT 1 cut(s) 2658
Asp700I GAANNNNTTC 2 cut(s) 348, 2385
AspLEI GCGC 3 cut(s) 76, 102, 3044
AspS9I GGNCC 9 cut(s) 168, 418, 1136, 1526, 1774, 1834, 2364, 2451, 2714
AsuC2I CCSGG 5 cut(s) 165, 166, 435, 2187, 2615
AsuHPI GGTGA 6 cut(s) 599, 1275, 1371, 1599, 1968, 2533
AvaI CYCGRG 2 cut(s) 164, 1248
AvaII GGWCC 7 cut(s) 168, 418, 1136, 1774, 1834, 2451, 2714
AxyI CCTNAGG 3 cut(s) 693, 1287, 1632
BaeGI GKGCMC 1 cut(s) 19
BalI TGGCCA 1 cut(s) 221
BamHI GGATCC 1 cut(s) 1675
BanI GGYRCC 2 cut(s) 14, 2066
BanII GRGCYC 1 cut(s) 267
Bbv12I GWGCWC 2 cut(s) 320, 3085
BccI CCATC 9 cut(s) 238, 722, 1378, 1928, 2300, 2403, 2549, 2828, 2974
BceAI ACGGC 4 cut(s) 308, 501, 838, 2454
BciVI GTATCC 1 cut(s) 3014
BclI TGATCA 2 cut(s) 1600, 2250
BcnI CCSGG 5 cut(s) 165, 166, 435, 2187, 2615
BcoDI GTCTC 7 cut(s) 646, 1215, 1238, 1371, 2306, 2499, 2853
BcuI ACTAGT 1 cut(s) 743
BfmI CTRYAG 2 cut(s) 1215, 2272
BfuAI ACCTGC 1 cut(s) 591
BfuI GTATCC 1 cut(s) 3014
BglII AGATCT 2 cut(s) 961, 2430
BlpI GCTNAGC 1 cut(s) 567
BmcAI AGTACT 1 cut(s) 1198
Bme18I GGWCC 7 cut(s) 168, 418, 1136, 1774, 1834, 2451, 2714
BmeT110I CYCGRG 2 cut(s) 164, 1248
BmgT120I GGNCC 9 cut(s) 168, 418, 1136, 1526, 1774, 1834, 2364, 2451, 2714
BmsI GCATC 8 cut(s) 407, 564, 1825, 2167, 2189, 2251, 2577, 2767
BoxI GACNNNNGTC 1 cut(s) 1250
BplI GAGNNNNNCTC 6 cut(s) 834, 866, 1144, 1176, 1973, 2005
BpmI CTGGAG 3 cut(s) 324, 744, 3008
Bpu1102I GCTNAGC 1 cut(s) 567
BpuEI CTTGAG 4 cut(s) 691, 1146, 3069, 3181
BpuMI CCSGG 5 cut(s) 165, 166, 435, 2187, 2615
BsaAI YACGTR 1 cut(s) 2085
BsaBI GATNNNNATC 2 cut(s) 1674, 2346
BsaI GGTCTC 1 cut(s) 1371
BsaWI WCCGGW 1 cut(s) 420
Bsc4I CCNNNNNNNGG 8 cut(s) 177, 694, 728, 920, 1535, 1577, 2373, 3179
Bse118I RCCGGY 2 cut(s) 76, 391
Bse1I ACTGG 6 cut(s) 234, 840, 890, 1207, 1314, 1648
Bse21I CCTNAGG 3 cut(s) 693, 1287, 1632
Bse3DI GCAATG 3 cut(s) 878, 1697, 2077
Bse8I GATNNNNATC 2 cut(s) 1674, 2346
BseGI GGATG 7 cut(s) 340, 398, 703, 1297, 2414, 2592, 2839
BseJI GATNNNNATC 2 cut(s) 1674, 2346
BseLI CCNNNNNNNGG 8 cut(s) 177, 694, 728, 920, 1535, 1577, 2373, 3179
BseMI GCAATG 3 cut(s) 878, 1697, 2077
BseMII CTCAG 6 cut(s) 228, 684, 1278, 1646, 2289, 3198
BseNI ACTGG 6 cut(s) 234, 840, 890, 1207, 1314, 1648
BseRI GAGGAG 6 cut(s) 401, 1149, 1270, 1373, 1540, 1551
BseSI GKGCMC 1 cut(s) 19
BseYI CCCAGC 3 cut(s) 198, 687, 1178
BsgI GTGCAG 2 cut(s) 616, 2653
Bsh1236I CGCG 2 cut(s) 74, 102
Bsh1285I CGRYCG 1 cut(s) 425
BshFI GGCC 8 cut(s) 83, 221, 406, 1156, 1528, 1762, 2365, 2724
BshNI GGYRCC 2 cut(s) 14, 2066
BsiEI CGRYCG 1 cut(s) 425
BsiHKAI GWGCWC 2 cut(s) 320, 3085
BsiHKCI CYCGRG 2 cut(s) 164, 1248
BsiSI CCGG 9 cut(s) 43, 77, 165, 392, 421, 434, 2187, 2454, 2614
BslFI GGGAC 4 cut(s) 181, 215, 1708, 1981
BslI CCNNNNNNNGG 8 cut(s) 177, 694, 728, 920, 1535, 1577, 2373, 3179
BsmAI GTCTC 7 cut(s) 646, 1215, 1238, 1371, 2306, 2499, 2853
BsmFI GGGAC 4 cut(s) 181, 215, 1708, 1981
BsmI GAATGC 1 cut(s) 2821
BsnI GGCC 8 cut(s) 83, 221, 406, 1156, 1528, 1762, 2365, 2724
Bso31I GGTCTC 1 cut(s) 1371
BsoBI CYCGRG 2 cut(s) 164, 1248
Bsp1286I GDGCHC 4 cut(s) 19, 267, 320, 3085
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp19I CCATGG 2 cut(s) 84, 1002
BspACI CCGC 8 cut(s) 80, 321, 395, 611, 659, 1702, 2016, 2362
BspANI GGCC 8 cut(s) 83, 221, 406, 1156, 1528, 1762, 2365, 2724
BspCNI CTCAG 6 cut(s) 229, 685, 1279, 1645, 2290, 3197
BspFNI CGCG 2 cut(s) 74, 102
BspHI TCATGA 2 cut(s) 2173, 2350
BspMI ACCTGC 1 cut(s) 591
BspPI GGATC 7 cut(s) 436, 495, 1622, 1644, 1670, 1683, 2786
BspQI GCTCTTC 2 cut(s) 308, 2420
BspT107I GGYRCC 2 cut(s) 14, 2066
BspTNI GGTCTC 1 cut(s) 1371
BsrDI GCAATG 3 cut(s) 878, 1697, 2077
BsrFI RCCGGY 2 cut(s) 76, 391
BsrI ACTGG 6 cut(s) 234, 840, 890, 1207, 1314, 1648
BssAI RCCGGY 2 cut(s) 76, 391
BssNAI GTATAC 1 cut(s) 1714
BssT1I CCWWGG 2 cut(s) 84, 1002
Bst1107I GTATAC 1 cut(s) 1714
Bst6I CTCTTC 8 cut(s) 308, 629, 1145, 1339, 1406, 2420, 2440, 2739
BstBAI YACGTR 1 cut(s) 2085
BstC8I GCNNGC 9 cut(s) 78, 393, 590, 1579, 2018, 2819, 2905, 3092, 3123
BstDSI CCRYGG 3 cut(s) 84, 324, 1002
BstEII GGTNACC 1 cut(s) 2935
BstF5I GGATG 7 cut(s) 340, 398, 703, 1297, 2414, 2592, 2839
BstFNI CGCG 2 cut(s) 74, 102
BstHHI GCGC 3 cut(s) 76, 102, 3044
BstMAI GTCTC 7 cut(s) 646, 1215, 1238, 1371, 2306, 2499, 2853
BstMCI CGRYCG 1 cut(s) 425
BstNSI RCATGY 1 cut(s) 1119
BstPAI GACNNNNGTC 1 cut(s) 1250
BstPI GGTNACC 1 cut(s) 2935
BstSFI CTRYAG 2 cut(s) 1215, 2272
BstSLI GKGCMC 1 cut(s) 19
BstUI CGCG 2 cut(s) 74, 102
BstX2I RGATCY 5 cut(s) 428, 487, 961, 1675, 2430
BstXI CCANNNNNNTGG 3 cut(s) 192, 229, 1133
BstYI RGATCY 5 cut(s) 428, 487, 961, 1675, 2430
BstZ17I GTATAC 1 cut(s) 1714
Bsu36I CCTNAGG 3 cut(s) 693, 1287, 1632
BsuI GTATCC 1 cut(s) 3014
BsuRI GGCC 8 cut(s) 83, 221, 406, 1156, 1528, 1762, 2365, 2724
BtgI CCRYGG 3 cut(s) 84, 324, 1002
BtgZI GCGATG 2 cut(s) 497, 2993
BtsCI GGATG 7 cut(s) 340, 398, 703, 1297, 2414, 2592, 2839
BtsI GCAGTG 3 cut(s) 599, 2332, 2683
BtsIMutI CAGTG 9 cut(s) 227, 234, 599, 1102, 1397, 1425, 2332, 2493, 2683
BveI ACCTGC 1 cut(s) 591
Cac8I GCNNGC 9 cut(s) 78, 393, 590, 1579, 2018, 2819, 2905, 3092, 3123
CaiI CAGNNNCTG 2 cut(s) 1427, 1871
CciI TCATGA 2 cut(s) 2173, 2350
CfoI GCGC 3 cut(s) 76, 102, 3044
Cfr10I RCCGGY 2 cut(s) 76, 391
Cfr13I GGNCC 9 cut(s) 168, 418, 1136, 1526, 1774, 1834, 2364, 2451, 2714
Cfr9I CCCGGG 1 cut(s) 164
CseI GACGC 3 cut(s) 80, 984, 3089
CsiI ACCWGGT 2 cut(s) 1830, 2716
Csp6I GTAC 5 cut(s) 249, 833, 1197, 1641, 2514
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CviQI GTAC 5 cut(s) 249, 833, 1197, 1641, 2514
DraI TTTAAA 2 cut(s) 930, 1660
EaeI YGGCCR 2 cut(s) 81, 219
Eam1104I CTCTTC 8 cut(s) 308, 629, 1145, 1339, 1406, 2420, 2440, 2739
EarI CTCTTC 8 cut(s) 308, 629, 1145, 1339, 1406, 2420, 2440, 2739
Eco130I CCWWGG 2 cut(s) 84, 1002
Eco147I AGGCCT 2 cut(s) 1156, 1762
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 1371
Eco47I GGWCC 7 cut(s) 168, 418, 1136, 1774, 1834, 2451, 2714
Eco57I CTGAAG 5 cut(s) 335, 839, 927, 1320, 3080
Eco81I CCTNAGG 3 cut(s) 693, 1287, 1632
Eco88I CYCGRG 2 cut(s) 164, 1248
Eco91I GGTNACC 1 cut(s) 2935
EcoO65I GGTNACC 1 cut(s) 2935
EcoRI GAATTC 1 cut(s) 1621
EcoT14I CCWWGG 2 cut(s) 84, 1002
EcoT22I ATGCAT 1 cut(s) 2182
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 84, 1002
FalI AAGNNNNNCTT 2 cut(s) 3078, 3110
FaqI GGGAC 4 cut(s) 181, 215, 1708, 1981
FauI CCCGC 3 cut(s) 618, 652, 2009
FauNDI CATATG 2 cut(s) 2854, 2973
FbaI TGATCA 2 cut(s) 1600, 2250
FblI GTMKAC 2 cut(s) 894, 1713
FokI GGATG 7 cut(s) 347, 385, 710, 1304, 2421, 2599, 2846
FriOI GRGCYC 1 cut(s) 267
FspI TGCGCA 1 cut(s) 3043
GlaI GCGC 3 cut(s) 75, 101, 3043
GsaI CCCAGC 3 cut(s) 202, 691, 1182
GsuI CTGGAG 3 cut(s) 324, 744, 3008
HaeIII GGCC 8 cut(s) 83, 221, 406, 1156, 1528, 1762, 2365, 2724
HapII CCGG 9 cut(s) 43, 77, 165, 392, 421, 434, 2187, 2454, 2614
HgaI GACGC 3 cut(s) 80, 984, 3089
HhaI GCGC 3 cut(s) 76, 102, 3044
Hin6I GCGC 3 cut(s) 74, 100, 3042
HinP1I GCGC 3 cut(s) 74, 100, 3042
HincII GTYRAC 3 cut(s) 766, 999, 1113
HindII GTYRAC 3 cut(s) 766, 999, 1113
HindIII AAGCTT 1 cut(s) 939
HpaI GTTAAC 1 cut(s) 1113
HpaII CCGG 9 cut(s) 43, 77, 165, 392, 421, 434, 2187, 2454, 2614
HphI GGTGA 6 cut(s) 599, 1275, 1371, 1599, 1968, 2533
Hpy166II GTNNAC 9 cut(s) 766, 791, 895, 999, 1113, 1301, 1471, 1714, 1976
Hpy8I GTNNAC 9 cut(s) 766, 791, 895, 999, 1113, 1301, 1471, 1714, 1976
Hpy99I CGWCG 2 cut(s) 74, 832
HpyAV CCTTC 8 cut(s) 268, 359, 642, 672, 863, 1053, 1884, 2396
HpyCH4IV ACGT 3 cut(s) 189, 1840, 2084
HpySE526I ACGT 3 cut(s) 189, 1840, 2084
HspAI GCGC 3 cut(s) 74, 100, 3042
KroI GCCGGC 2 cut(s) 76, 391
KroNI GCCGGC 2 cut(s) 78, 393
Ksp22I TGATCA 2 cut(s) 1600, 2250
KspAI GTTAAC 1 cut(s) 1113
LguI GCTCTTC 2 cut(s) 308, 2420
LmnI GCTCC 9 cut(s) 388, 855, 1283, 1538, 1553, 1813, 2983, 2989, 3196
LweI GCATC 8 cut(s) 407, 564, 1825, 2167, 2189, 2251, 2577, 2767
MabI ACCWGGT 2 cut(s) 1830, 2716
MaeII ACGT 3 cut(s) 189, 1840, 2084
MaeIII GTNAC 9 cut(s) 536, 700, 1091, 1377, 1499, 1686, 2096, 2678, 2935
MfeI CAATTG 1 cut(s) 2946
MflI RGATCY 5 cut(s) 428, 487, 961, 1675, 2430
MhlI GDGCHC 4 cut(s) 19, 267, 320, 3085
MlsI TGGCCA 1 cut(s) 221
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 8 cut(s) 67, 544, 1240, 1260, 1472, 1690, 2303, 2585
MmeI TCCRAC 3 cut(s) 1416, 1861, 2095
Mox20I TGGCCA 1 cut(s) 221
Mph1103I ATGCAT 1 cut(s) 2182
MreI CGCCGGCG 1 cut(s) 76
MroNI GCCGGC 2 cut(s) 76, 391
MroXI GAANNNNTTC 2 cut(s) 348, 2385
MscI TGGCCA 1 cut(s) 221
MseI TTAA 9 cut(s) 929, 1112, 1659, 1853, 1985, 2229, 2609, 2658, 2942
MslI CAYNNNNRTG 6 cut(s) 239, 323, 1932, 2087, 2213, 2355
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 5 cut(s) 177, 355, 1133, 1178, 2605
MspI CCGG 9 cut(s) 43, 77, 165, 392, 421, 434, 2187, 2454, 2614
MssI GTTTAAAC 1 cut(s) 1660
MunI CAATTG 1 cut(s) 2946
Mva1269I GAATGC 1 cut(s) 2821
MvnI CGCG 2 cut(s) 74, 102
NaeI GCCGGC 2 cut(s) 78, 393
NciI CCSGG 5 cut(s) 165, 166, 435, 2187, 2615
NcoI CCATGG 2 cut(s) 84, 1002
NdeI CATATG 2 cut(s) 2854, 2973
NgoMIV GCCGGC 2 cut(s) 76, 391
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 4 cut(s) 536, 1091, 1377, 2678
NsbI TGCGCA 1 cut(s) 3043
NsiI ATGCAT 1 cut(s) 2182
NspI RCATGY 1 cut(s) 1119
OliI CACNNNNGTG 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 1248
PagI TCATGA 2 cut(s) 2173, 2350
PceI AGGCCT 2 cut(s) 1156, 1762
PciSI GCTCTTC 2 cut(s) 308, 2420
PctI GAATGC 1 cut(s) 2821
PdiI GCCGGC 2 cut(s) 78, 393
PdmI GAANNNNTTC 2 cut(s) 348, 2385
PfeI GAWTC 8 cut(s) 358, 859, 916, 1223, 1868, 1942, 2074, 2284
PflFI GACNNNGTC 1 cut(s) 1427
PflMI CCANNNNNTGG 2 cut(s) 177, 3179
PleI GAGTC 8 cut(s) 66, 543, 1240, 1259, 1472, 1689, 2302, 2584
PmeI GTTTAAAC 1 cut(s) 1660
PpsI GAGTC 8 cut(s) 66, 543, 1240, 1259, 1472, 1689, 2302, 2584
Ppu21I YACGTR 1 cut(s) 2085
PshAI GACNNNNGTC 1 cut(s) 1250
PshBI ATTAAT 1 cut(s) 2658
Psp1406I AACGTT 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 2935
PspFI CCCAGC 3 cut(s) 198, 687, 1178
PspPI GGNCC 9 cut(s) 168, 418, 1136, 1526, 1774, 1834, 2364, 2451, 2714
PspXI VCTCGAGB 1 cut(s) 1248
PstNI CAGNNNCTG 2 cut(s) 1427, 1871
PsuI RGATCY 5 cut(s) 428, 487, 961, 1675, 2430
PsyI GACNNNGTC 1 cut(s) 1427
PvuII CAGCTG 5 cut(s) 177, 355, 1133, 1178, 2605
RsaI GTAC 5 cut(s) 250, 834, 1198, 1642, 2515
RsaNI GTAC 5 cut(s) 249, 833, 1197, 1641, 2514
RseI CAYNNNNRTG 6 cut(s) 239, 323, 1932, 2087, 2213, 2355
SapI GCTCTTC 2 cut(s) 308, 2420
SaqAI TTAA 9 cut(s) 929, 1112, 1659, 1853, 1985, 2229, 2609, 2658, 2942
Sau96I GGNCC 9 cut(s) 168, 418, 1136, 1526, 1774, 1834, 2364, 2451, 2714
ScaI AGTACT 1 cut(s) 1198
SchI GAGTC 8 cut(s) 67, 544, 1240, 1260, 1472, 1690, 2303, 2585
SduI GDGCHC 4 cut(s) 19, 267, 320, 3085
SexAI ACCWGGT 2 cut(s) 1830, 2716
SfaNI GCATC 8 cut(s) 407, 564, 1825, 2167, 2189, 2251, 2577, 2767
SfcI CTRYAG 2 cut(s) 1215, 2272
Sfr274I CTCGAG 1 cut(s) 1248
SgrAI CRCCGGYG 1 cut(s) 76
SinI GGWCC 7 cut(s) 168, 418, 1136, 1774, 1834, 2451, 2714
SlaI CTCGAG 1 cut(s) 1248
SmaI CCCGGG 1 cut(s) 166
SmiI ATTTAAAT 1 cut(s) 930
SmiMI CAYNNNNRTG 6 cut(s) 239, 323, 1932, 2087, 2213, 2355
SmlI CTYRAG 5 cut(s) 706, 1161, 1248, 3084, 3160
SmoI CTYRAG 5 cut(s) 706, 1161, 1248, 3084, 3160
SpeI ACTAGT 1 cut(s) 743
SseBI AGGCCT 2 cut(s) 1156, 1762
SsiI CCGC 8 cut(s) 80, 321, 395, 611, 659, 1702, 2016, 2362
StuI AGGCCT 2 cut(s) 1156, 1762
StyI CCWWGG 2 cut(s) 84, 1002
SwaI ATTTAAAT 1 cut(s) 930
TaiI ACGT 3 cut(s) 192, 1843, 2087
TaqI TCGA 3 cut(s) 56, 1249, 2553
TatI WGTACW 2 cut(s) 1196, 2513
TauI GCSGC 3 cut(s) 83, 398, 2365
TfiI GAWTC 8 cut(s) 358, 859, 916, 1223, 1868, 1942, 2074, 2284
Tru1I TTAA 9 cut(s) 929, 1112, 1659, 1853, 1985, 2229, 2609, 2658, 2942
Tru9I TTAA 9 cut(s) 929, 1112, 1659, 1853, 1985, 2229, 2609, 2658, 2942
TscAI CASTG 9 cut(s) 234, 241, 599, 1102, 1404, 1432, 2332, 2500, 2683
TseFI GTSAC 4 cut(s) 536, 1091, 1377, 2678
Tsp45I GTSAC 4 cut(s) 536, 1091, 1377, 2678
TspGWI ACGGA 1 cut(s) 1725
TspMI CCCGGG 1 cut(s) 164
TspRI CASTG 9 cut(s) 234, 241, 599, 1102, 1404, 1432, 2332, 2500, 2683
Tth111I GACNNNGTC 1 cut(s) 1427
Van91I CCANNNNNTGG 2 cut(s) 177, 3179
VpaK11BI GGWCC 7 cut(s) 168, 418, 1136, 1774, 1834, 2451, 2714
VspI ATTAAT 1 cut(s) 2658
XbaI TCTAGA 1 cut(s) 2533
XceI RCATGY 1 cut(s) 1119
XhoI CTCGAG 1 cut(s) 1248
XmaI CCCGGG 1 cut(s) 164
XmiI GTMKAC 2 cut(s) 894, 1713
XmnI GAANNNNTTC 2 cut(s) 348, 2385
ZrmI AGTACT 1 cut(s) 1198
Zsp2I ATGCAT 1 cut(s) 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.