Rorug02G0441200

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000444
Physical Location & Seq
Forward (+)
56583958 .. 56587466
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0441200.1

Sequence Viewer

Length: 531 bp
ATGGCAGGGGAAGATGAGGACTTACCAAGAGACGCAAAGATTGTGAAGACGCTGTTAAAATCAATGGGCGTGGAGGACTATGAACCTCGCGTTATTCACCAATTCTTGGAGCTATGGTATCGGTACGTGGTCGATGTACTCAGTGATGCTCAAGTTTACTCTGATCACGCGGGCAAGGGTGCGATTGATTGCGATGATATCAAGCTTGCCATTCAGTCCAAGGTCAATTTCAGCTTCTCGCAACCCCCTCCAAGAGAGGTTCTACTGGAGTTGGCCAGAAACAGGAACAAAATTCCATTACCAAAATCCATCTCAGGGCCTGGTGTGGCGCTACCACCTGACCAGGATACCTTGATCAGCCCAAATTATCAACTGGCAGTCCCTAGGAAACGACCAGCTCAAGCAGTTGAAGAAATGGAAGAGGATGAAGAAACTGTTGAACCCAATCCACCCCAAGAGCAGAAGCCTACTGATATACCGCAGAATACATCCCAAAAGGTATCTTTTCCCCTTGCTAAACGCACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

176

Amino Acids

19.8

Weight (kDa)

4.95

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIID-31kDa PF02291 6 - 125 3.7e-49 Transcription initiation factor IID, 31kD subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 106
AccII CGCG 2 cut(s) 90, 170
AciI CCGC 2 cut(s) 170, 479
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 528
AfaI GTAC 2 cut(s) 125, 138
AfiI CCNNNNNNNGG 3 cut(s) 106, 282, 315
AgsI TTSAA 2 cut(s) 410, 440
AjnI CCWGG 2 cut(s) 319, 342
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 4 cut(s) 112, 205, 234, 398
AluI AGCT 4 cut(s) 112, 205, 234, 398
Alw26I GTCTC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 2 cut(s) 273, 317
ApoI RAATTY 1 cut(s) 291
AspA2I CCTAGG 1 cut(s) 383
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 317
AsuHPI GGTGA 1 cut(s) 89
AvrII CCTAGG 1 cut(s) 383
BalI TGGCCA 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 53
BccI CCATC 1 cut(s) 317
BciT130I CCWGG 2 cut(s) 321, 344
BciVI GTATCC 1 cut(s) 340
BclI TGATCA 2 cut(s) 163, 354
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 1 cut(s) 384
BfoI RGCGCY 1 cut(s) 332
BfuI GTATCC 1 cut(s) 340
BlnI CCTAGG 1 cut(s) 383
Bme1390I CCNGG 2 cut(s) 321, 344
BmgT120I GGNCC 1 cut(s) 317
BmrFI CCNGG 2 cut(s) 321, 344
BmsI GCATC 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 135, 384
BsaAI YACGTR 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 219, 383
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 282, 315
Bse1I ACTGG 2 cut(s) 270, 378
BseBI CCWGG 2 cut(s) 321, 344
BseDI CCNNGG 2 cut(s) 219, 383
BseGI GGATG 2 cut(s) 430, 488
BseLI CCNNNNNNNGG 3 cut(s) 106, 282, 315
BseMII CTCAG 2 cut(s) 154, 327
BseNI ACTGG 2 cut(s) 270, 378
Bsh1236I CGCG 2 cut(s) 90, 170
BshFI GGCC 2 cut(s) 275, 319
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 3 cut(s) 106, 282, 315
BsmAI GTCTC 1 cut(s) 24
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 2 cut(s) 275, 319
Bsp143I GATC 2 cut(s) 163, 354
BspACI CCGC 2 cut(s) 170, 479
BspANI GGCC 2 cut(s) 275, 319
BspCNI CTCAG 2 cut(s) 153, 326
BspFNI CGCG 2 cut(s) 90, 170
BsrI ACTGG 2 cut(s) 270, 378
BssECI CCNNGG 2 cut(s) 219, 383
BssMI GATC 2 cut(s) 163, 354
BssT1I CCWWGG 2 cut(s) 219, 383
Bst2UI CCWGG 2 cut(s) 321, 344
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 127
BstC8I GCNNGC 2 cut(s) 172, 207
BstDEI CTNAG 2 cut(s) 140, 313
BstF5I GGATG 2 cut(s) 430, 488
BstFNI CGCG 2 cut(s) 90, 170
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 2 cut(s) 166, 357
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 2 cut(s) 163, 354
BstNI CCWGG 2 cut(s) 321, 344
BstSCI CCNGG 2 cut(s) 319, 342
BstUI CGCG 2 cut(s) 90, 170
BstV2I GAAGAC 1 cut(s) 53
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 2 cut(s) 275, 319
BtgZI GCGATG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 430, 488
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 2 cut(s) 172, 207
CaiI CAGNNNCTG 1 cut(s) 320
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 317
CseI GACGC 2 cut(s) 41, 58
Csp6I GTAC 2 cut(s) 124, 137
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviJI RGCY 8 cut(s) 112, 205, 234, 275, 319, 360, 398, 466
CviKI_1 RGCY 8 cut(s) 112, 205, 234, 275, 319, 360, 398, 466
CviQI GTAC 2 cut(s) 124, 137
DdeI CTNAG 2 cut(s) 140, 313
DpnI GATC 2 cut(s) 165, 356
DpnII GATC 2 cut(s) 163, 354
DraIII CACNNNGTG 1 cut(s) 528
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 219, 383
Eco32I GATATC 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 317
EcoRII CCWGG 2 cut(s) 319, 342
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 219, 383
ErhI CCWWGG 2 cut(s) 219, 383
Esp3I CGTCTC 1 cut(s) 24
FaiI YATR 3 cut(s) 81, 115, 476
FaqI GGGAC 1 cut(s) 365
FauI CCCGC 1 cut(s) 163
FbaI TGATCA 2 cut(s) 163, 354
FokI GGATG 2 cut(s) 437, 475
FspBI CTAG 1 cut(s) 384
GlaI GCGC 1 cut(s) 330
GsuI CTGGAG 1 cut(s) 287
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 2 cut(s) 275, 319
HgaI GACGC 2 cut(s) 41, 58
HhaI GCGC 1 cut(s) 331
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 203
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 163
Hpy8I GTNNAC 1 cut(s) 157
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 126
HpyF3I CTNAG 2 cut(s) 140, 313
HpySE526I ACGT 1 cut(s) 126
HspAI GCGC 1 cut(s) 329
Ksp22I TGATCA 2 cut(s) 163, 354
Kzo9I GATC 2 cut(s) 163, 354
LmnI GCTCC 1 cut(s) 109
LweI GCATC 1 cut(s) 136
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 126
MalI GATC 2 cut(s) 165, 356
MboI GATC 2 cut(s) 163, 354
MboII GAAGA 5 cut(s) 23, 58, 422, 431, 440
MlsI TGGCCA 1 cut(s) 275
MluCI AATT 4 cut(s) 101, 226, 291, 364
MluNI TGGCCA 1 cut(s) 275
MnlI CCTC 6 cut(s) 10, 67, 96, 250, 258, 415
Mox20I TGGCCA 1 cut(s) 275
MscI TGGCCA 1 cut(s) 275
MseI TTAA 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 321, 344
MvaI CCWGG 2 cut(s) 321, 344
MvnI CGCG 2 cut(s) 90, 170
NdeII GATC 2 cut(s) 163, 354
PflMI CCANNNNNTGG 1 cut(s) 106
Ppu21I YACGTR 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 319, 342
PspGI CCWGG 2 cut(s) 319, 342
PspPI GGNCC 1 cut(s) 317
PstNI CAGNNNCTG 1 cut(s) 320
RsaI GTAC 2 cut(s) 125, 138
RsaNI GTAC 2 cut(s) 124, 137
SaqAI TTAA 1 cut(s) 56
Sau3AI GATC 2 cut(s) 163, 354
Sau96I GGNCC 1 cut(s) 317
ScrFI CCNGG 2 cut(s) 321, 344
SfaNI GCATC 1 cut(s) 136
SmlI CTYRAG 2 cut(s) 150, 399
SmoI CTYRAG 2 cut(s) 150, 399
Sse9I AATT 4 cut(s) 101, 226, 291, 364
SsiI CCGC 2 cut(s) 170, 479
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 2 cut(s) 319, 342
StyI CCWWGG 2 cut(s) 219, 383
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 132
TasI AATT 4 cut(s) 101, 226, 291, 364
TatI WGTACW 1 cut(s) 136
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 96, 441
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 106
XapI RAATTY 1 cut(s) 291
XmaJI CCTAGG 1 cut(s) 383
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.