MD09G1137900.v1.1

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000444
Physical Location & Seq
Forward (+)
10712793 .. 10718351
5559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1137900.v1.1.491

Sequence Viewer

Length: 2754 bp
ATGATATCTTTGGAGGGTTTCGAGCCCGTAGCTGTTTCTCCCCAGAAACAAGACCCGGCATGGAAACACTGCCAAGTTTTCATGAAAGACCAACCCAATGGGGTTAGAGTTGAGTTGAAAAAGTGTATATATTGCGGCAAGTTGTTTCAGGGTGGTGGGATTACTCGGCTTAAATCCCACCTTGCCGGTCGGAAGGGCAATGGCCCCACCTGTGAGCAGGCCCCGCCGGATGTTAGAATCGCAATGTTGCAGAGTTTGGATGATGGGATTGCAGCGTTTAGGCAGAGGAAGAGTCGAATTGTTGCTAACCCGCATTGTGGCATTAGCGAAATTGACGGTTCATTAGGCGAAAATGGAGAGTATAAATTGGTTGTAGGACACTTTTCGAATGCGGTGGTGAATGATGAGGAGGCTGGTGGGATGAGTGGGAATGGAGGAATTAGGTTAAGTGATAATTTGACGAATGTGCCCCTTGAAGTAGATAATTCTTACAGGAGTAATGCTCAGCAGGGATTAATTGGAGTGCCCAAATTGGATTTTTCGGTAGATGAAGAGGAAGATGGTGGGACAAGTGTTGATAGAAGAGTACAGCGGAGGGGTAGACCTTCAGGTGGTGCTACCTGTTCTGGTTCAGTTCATGCTGGTGTTACGAAATTGAGTAGTGTTTCAAATCCTTTTCCTATTTCAGTAGGCAATGCTTTTCCCAATTCAAATTTATCGCAAAACGAAGAAGAAGTTGGCATCAAACTGAATCAGGAAGATGTTGGATTGAGTATTGATAGAAGAGTAAGGCGGAGGGGTAGACCTTCTAAAGCAGCAACTGGTTCAGTTAATGCTGTTATGAAATTGAGTAATGCTTCACCTCCTGTTCCCATTGCATTAGGTAATGCTTTTCCCAATTCAAATTTGTTGGAAAATGAAGACGTTGGCATAAGTAATACAAATGTGAGTGTGAAGAAGAGGGTAAGGGATGAAAATGCTTTTGGGGCTGCGAATGATGGAACCATCGGCAATGATTACGAGGTAAAAAGGTTGAGCATTGAACAAATACATATGGCAATTGGTCGGTTTTTGTATGAAATTCAGGCTCCCCTGGATGCCGTGAAGAACTCTGTTTATTTCCAGCCAATGATTGACGCAATTGCCTCTGGGGGAAAGGAGACTGTGGCACCCTCATATGATGACATTCGGGGTTGGATGTTGAAGAATGCAGTTGAAGAGATGAAAAGTGAAATTCAACAACACAAGGAAACTTGGGCGAGGACAGGTTGTTCTCTTCTAGTCAGCCAATGGAATTCTGAAAAGGGAAGAACTTTGCTAAATTTTGCTGTGTACTCTTCTGAAGGAACAGCCTATTTGAAATCTGTGGATGCTTCCTACTTTATTTTTTCCCCAGATGCTTTGTTTGCATTTTTCAAGGAAATGGTGGAAGAAATTGGAGTGGTACATGTTTTGCAAGTCATTACAAATACTGAAGAGCAGTTTTCTGTTGTTGGGAAGAAGTTGATGGATACGTTCCCTACCCTGTACTGGTCTCCCTGTTCCACTGCTTGCATAGATTCGATTCTTGAGGATTTCGGAAAAGTTGAGTGGATAAATTCTGTAATTGAACAGGCTAGATCCGTTACAAGATTTATCTACAAACACGTTGTCATTTTGAACATGATGAGAAGGTATACGTTCGGGAATGACATTGTTAGGCTGGGGGTATCTAGGTTTGCAACAAACTTTATGACGTTGAAACAAATGGCTGGCCTTAAATATAATTTGCAGTCGATGGTTACTTCAAATGAGTGGATGGGCTGCCCCTTTTCTAAAACACCAGAAGGATCGGCAGTGTTAGATATATTCAGTAATCACTCATTTTGGTCTTCGTGCATTTTGATCACCCGTTTAACAAATCCACTCTTGCGAGTTTTGAGAATTGTTGGTAGCCAGAAGAGGCCTGCGATGGGATATGTTTTTGCAGGGATGTATCGAGCAAAAGAAACAATCAAAATAGAACTTGTCAAGAAAGAAGAATATATGGTGTACTGGAACATTATAGATCACAGATGGAAAAAACTGTGGGATTTTCCCCTTCACGCTGCAGGATTTTACCTTAATCCCAAGTTCTTTTATGGCTTCAAAGGAGACATGCATAATGAAATTGTGTCGAGGATGTTTGACTGCATAGAGAGATTGGTTCCTGATATAAAAGTCCAAGATGAAGTAATCAAAGAGATAAACTTGTACAAAAATGCTGTTGGAGATTTGGGGAGGAATTTGGCAGTCCGAGCAAGGGACAATCTGCTTCCTGCTGAATGGTGGTCTACATATGGTGGTGGTTGCCCAAACCTTGCACGCTTGGCAATTCGTATTCTCAGTCAAACTTGCAGCTTAGTTCCGTGTCAGGATCATCAGATTCCTTTCGAACAGTTGCACAAGACAAGGAACAGCGTAGAACATCAGCGGCTTAATGATCTTGTTTTCGTTCAATACAACCTCAAACTAAAACAGAAGGTTCATAAGCACAAAGAACAGGAGTACGCGGATCCTATATCATTTGACCGCAATAGCATTGCCGAAGAGTGGGTAGCAGAGATGGAGATGCACCAAGAAGACACTGAAAATCCTGATTGGATGTCACTTGATCCCCCCTCTGAGAACTCGAGGCTTTTAGAACTTTCGGTTGATGAAGTTGAAGAATTGGGTTCAGGGTTTGATGACAATGAAATTTTGAGCGGATTGAAAGTGGTTAAAGAAGAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

918

Amino Acids

102.71

Weight (kDa)

5.97

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 391 - 542 1.8e-52 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 754 - 829 3.8e-09 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1168
AccBSI CCGCTC 1 cut(s) 2724
AccI GTMKAC 4 cut(s) 601, 802, 1676, 2312
AccII CGCG 1 cut(s) 2531
AclWI GGATC 6 cut(s) 1614, 1837, 2403, 2528, 2541, 2627
AcsI RAATTY 9 cut(s) 712, 904, 1080, 1233, 1294, 1321, 1597, 2263, 2715
AcuI CTGAAG 3 cut(s) 591, 1362, 1494
AfaI GTAC 7 cut(s) 588, 1334, 1446, 1529, 2033, 2234, 2528
AfiI CCNNNNNNNGG 6 cut(s) 317, 611, 1530, 1952, 2281, 2571
AflIII ACRYGT 2 cut(s) 1447, 1645
AjnI CCWGG 1 cut(s) 1092
AjuI GAANNNNNNNTTGG 4 cut(s) 720, 752, 966, 998
AloI GAACNNNNNNTCC 2 cut(s) 1255, 1287
AluBI AGCT 2 cut(s) 32, 2379
AluI AGCT 2 cut(s) 32, 2379
Alw26I GTCTC 3 cut(s) 1154, 1539, 2127
AlwI GGATC 6 cut(s) 1614, 1837, 2403, 2528, 2541, 2627
AlwNI CAGNNNCTG 1 cut(s) 821
Ama87I CYCGRG 1 cut(s) 2650
AoxI GGCC 4 cut(s) 202, 219, 1753, 1943
ApeKI GCWGC 6 cut(s) 272, 815, 989, 1803, 2087, 2376
ApoI RAATTY 9 cut(s) 712, 904, 1080, 1233, 1294, 1321, 1597, 2263, 2715
ArsI GACNNNNNNTTYG 2 cut(s) 1635, 1667
AseI ATTAAT 1 cut(s) 515
AspS9I GGNCC 2 cut(s) 203, 220
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 409, 852, 1879
AsuII TTCGAA 2 cut(s) 386, 2412
AvaI CYCGRG 1 cut(s) 2650
BaeGI GKGCMC 2 cut(s) 471, 528
BamHI GGATCC 1 cut(s) 2533
BanI GGYRCC 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 3 cut(s) 927, 1863, 2607
BbvI GCAGC 6 cut(s) 284, 827, 976, 1790, 2074, 2388
BceAI ACGGC 1 cut(s) 1085
BciT130I CCWGG 1 cut(s) 1094
BciVI GTATCC 1 cut(s) 1504
BclI TGATCA 1 cut(s) 1884
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 3 cut(s) 1154, 1539, 2127
BfaI CTAG 3 cut(s) 1280, 1617, 1713
BfmI CTRYAG 1 cut(s) 2088
BfuI GTATCC 1 cut(s) 1504
BisI GCNGC 8 cut(s) 136, 273, 816, 990, 1804, 2088, 2377, 2453
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 8 cut(s) 137, 274, 817, 991, 1805, 2089, 2378, 2454
Bme1390I CCNGG 2 cut(s) 56, 1094
BmeT110I CYCGRG 1 cut(s) 2650
BmgT120I GGNCC 2 cut(s) 203, 220
BmiI GGNNCC 7 cut(s) 205, 222, 1003, 1089, 1170, 2187, 2535
BmrFI CCNGG 2 cut(s) 56, 1094
BmsI GCATC 5 cut(s) 750, 1087, 1360, 1387, 2580
BpiI GAAGAC 3 cut(s) 927, 1863, 2607
BplI GAGNNNNNCTC 2 cut(s) 487, 519
Bpu1102I GCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 2 cut(s) 386, 2412
BpuEI CTTGAG 1 cut(s) 1589
BpuMI CCSGG 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 1539
BsaJI CCNNGG 1 cut(s) 1092
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 611, 1530, 1952, 2281, 2571
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 826, 1535, 2039
Bse3DI GCAATG 6 cut(s) 205, 249, 700, 873, 1018, 2559
BseBI CCWGG 1 cut(s) 1094
BseDI CCNNGG 1 cut(s) 1092
BseLI CCNNNNNNNGG 6 cut(s) 317, 611, 1530, 1952, 2281, 2571
BseMI GCAATG 6 cut(s) 205, 249, 700, 873, 1018, 2559
BseMII CTCAG 3 cut(s) 518, 2377, 2634
BseNI ACTGG 3 cut(s) 826, 1535, 2039
BseRI GAGGAG 1 cut(s) 422
BseSI GKGCMC 2 cut(s) 471, 528
BseXI GCAGC 6 cut(s) 284, 827, 976, 1790, 2074, 2388
BseYI CCCAGC 1 cut(s) 1702
Bsh1236I CGCG 1 cut(s) 2531
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 4 cut(s) 204, 221, 1755, 1945
BshNI GGYRCC 1 cut(s) 1168
BsiEI CGRYCG 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 2650
BsiSI CCGG 3 cut(s) 56, 186, 227
BslFI GGGAC 2 cut(s) 580, 2297
BslI CCNNNNNNNGG 6 cut(s) 317, 611, 1530, 1952, 2281, 2571
BsmAI GTCTC 3 cut(s) 1154, 1539, 2127
BsmFI GGGAC 2 cut(s) 580, 2297
BsmI GAATGC 2 cut(s) 394, 1213
BsnI GGCC 4 cut(s) 204, 221, 1755, 1945
Bso31I GGTCTC 1 cut(s) 1539
BsoBI CYCGRG 1 cut(s) 2650
Bsp119I TTCGAA 2 cut(s) 386, 2412
Bsp1286I GDGCHC 3 cut(s) 27, 471, 528
Bsp1407I TGTACA 1 cut(s) 2232
Bsp143I GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
Bsp1720I GCTNAGC 1 cut(s) 504
BspANI GGCC 4 cut(s) 204, 221, 1755, 1945
BspCNI CTCAG 3 cut(s) 517, 2376, 2635
BspFNI CGCG 1 cut(s) 2531
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 7 cut(s) 205, 222, 1003, 1089, 1170, 2187, 2535
BspMAI CTGCAG 1 cut(s) 2092
BspPI GGATC 6 cut(s) 1614, 1837, 2403, 2528, 2541, 2627
BspQI GCTCTTC 1 cut(s) 1470
BspT104I TTCGAA 2 cut(s) 386, 2412
BspT107I GGYRCC 1 cut(s) 1168
BspTNI GGTCTC 1 cut(s) 1539
BsrBI CCGCTC 1 cut(s) 2724
BsrDI GCAATG 6 cut(s) 205, 249, 700, 873, 1018, 2559
BsrFI RCCGGY 1 cut(s) 185
BsrGI TGTACA 1 cut(s) 2232
BsrI ACTGG 3 cut(s) 826, 1535, 2039
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 1092
BssMI GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
BssNAI GTATAC 1 cut(s) 1677
Bst1107I GTATAC 1 cut(s) 1677
Bst2UI CCWGG 1 cut(s) 1094
Bst4CI ACNGT 4 cut(s) 338, 1165, 2067, 2418
BstAUI TGTACA 1 cut(s) 2232
BstBI TTCGAA 2 cut(s) 386, 2412
BstC8I GCNNGC 5 cut(s) 219, 1552, 1753, 1947, 2344
BstDEI CTNAG 4 cut(s) 504, 2363, 2380, 2643
BstFNI CGCG 1 cut(s) 2531
BstKTI GATC 8 cut(s) 1622, 1832, 1887, 2050, 2398, 2464, 2536, 2635
BstMAI GTCTC 3 cut(s) 1154, 1539, 2127
BstMBI GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 5 cut(s) 223, 986, 1406, 2276, 2348
BstNI CCWGG 1 cut(s) 1094
BstNSI RCATGY 2 cut(s) 1451, 2140
BstSCI CCNGG 2 cut(s) 54, 1092
BstSFI CTRYAG 1 cut(s) 2088
BstSLI GKGCMC 2 cut(s) 471, 528
BstUI CGCG 1 cut(s) 2531
BstV1I GCAGC 6 cut(s) 284, 827, 976, 1790, 2074, 2388
BstV2I GAAGAC 3 cut(s) 927, 1863, 2607
BstX2I RGATCY 2 cut(s) 1619, 2533
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 2 cut(s) 1619, 2533
BstZ17I GTATAC 1 cut(s) 1677
BsuI GTATCC 1 cut(s) 1504
BsuRI GGCC 4 cut(s) 204, 221, 1755, 1945
BtgZI GCGATG 1 cut(s) 1964
BtsI GCAGTG 3 cut(s) 67, 1545, 1842
BtsIMutI CAGTG 4 cut(s) 67, 1545, 1842, 2604
Cac8I GCNNGC 5 cut(s) 219, 1552, 1753, 1947, 2344
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 203, 220
CseI GACGC 1 cut(s) 1145
Csp6I GTAC 7 cut(s) 587, 1333, 1445, 1528, 2032, 2233, 2527
CviAII CATG 6 cut(s) 60, 82, 638, 1448, 1663, 2137
CviQI GTAC 7 cut(s) 587, 1333, 1445, 1528, 2032, 2233, 2527
DdeI CTNAG 4 cut(s) 504, 2363, 2380, 2643
DpnI GATC 8 cut(s) 1621, 1831, 1886, 2049, 2397, 2463, 2535, 2634
DpnII GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
EciI GGCGGA 1 cut(s) 808
Eco147I AGGCCT 1 cut(s) 1945
Eco24I GRGCYC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 1539
Eco32I GATATC 1 cut(s) 6
Eco57I CTGAAG 3 cut(s) 591, 1362, 1494
Eco88I CYCGRG 1 cut(s) 2650
EcoO109I RGGNCCY 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 1294
EcoRII CCWGG 1 cut(s) 1092
EcoRV GATATC 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 2142
EcoT38I GRGCYC 1 cut(s) 27
FaeI CATG 6 cut(s) 63, 85, 641, 1451, 1666, 2140
FaqI GGGAC 2 cut(s) 580, 2297
FatI CATG 6 cut(s) 59, 81, 637, 1447, 1662, 2136
FauI CCCGC 2 cut(s) 231, 318
FauNDI CATATG 3 cut(s) 1053, 1177, 2317
FbaI TGATCA 1 cut(s) 1884
FblI GTMKAC 4 cut(s) 601, 802, 1676, 2312
Fnu4HI GCNGC 8 cut(s) 136, 273, 816, 990, 1804, 2088, 2377, 2453
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 8 cut(s) 136, 273, 816, 990, 1804, 2088, 2377, 2453
FspBI CTAG 3 cut(s) 1280, 1617, 1713
GluI GCNGC 8 cut(s) 136, 273, 816, 990, 1804, 2088, 2377, 2453
GsaI CCCAGC 1 cut(s) 1706
HaeIII GGCC 4 cut(s) 204, 221, 1755, 1945
HapII CCGG 3 cut(s) 56, 186, 227
HgaI GACGC 1 cut(s) 1145
Hin1II CATG 6 cut(s) 63, 85, 641, 1451, 1666, 2140
HinfI GANTC 6 cut(s) 237, 292, 751, 1559, 1564, 2404
HpaII CCGG 3 cut(s) 56, 186, 227
HphI GGTGA 3 cut(s) 409, 852, 1879
Hpy166II GTNNAC 6 cut(s) 602, 803, 1333, 1677, 2032, 2313
Hpy188I TCNGA 7 cut(s) 192, 1300, 1342, 1580, 2276, 2403, 2644
Hpy188III TCNNGA 8 cut(s) 82, 755, 1568, 1684, 2011, 2189, 2393, 2615
Hpy8I GTNNAC 6 cut(s) 602, 803, 1333, 1677, 2032, 2313
HpyAV CCTTC 8 cut(s) 187, 615, 816, 1337, 1665, 1820, 2090, 2494
HpyCH4III ACNGT 4 cut(s) 338, 1165, 2067, 2418
HpyCH4IV ACGT 5 cut(s) 924, 1514, 1647, 1679, 1736
HpyF10VI GCNNNNNNNGC 5 cut(s) 223, 986, 1406, 2276, 2348
HpyF3I CTNAG 4 cut(s) 504, 2363, 2380, 2643
HpySE526I ACGT 5 cut(s) 924, 1514, 1647, 1679, 1736
Hsp92II CATG 6 cut(s) 63, 85, 641, 1451, 1666, 2140
Ksp22I TGATCA 1 cut(s) 1884
Kzo9I GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
LguI GCTCTTC 1 cut(s) 1470
LmnI GCTCC 1 cut(s) 1093
Lsp1109I GCAGC 6 cut(s) 284, 827, 976, 1790, 2074, 2388
LweI GCATC 5 cut(s) 750, 1087, 1360, 1387, 2580
MaeI CTAG 3 cut(s) 1280, 1617, 1713
MaeII ACGT 5 cut(s) 924, 1514, 1647, 1679, 1736
MaeIII GTNAC 4 cut(s) 646, 1624, 1780, 2625
MalI GATC 8 cut(s) 1621, 1831, 1886, 2049, 2397, 2463, 2535, 2634
MbiI CCGCTC 1 cut(s) 2724
MboI GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
MfeI CAATTG 2 cut(s) 1059, 1140
MflI RGATCY 2 cut(s) 1619, 2533
MhlI GDGCHC 3 cut(s) 27, 471, 528
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 5 cut(s) 170, 745, 891, 1175, 2227
Mph1103I ATGCAT 1 cut(s) 2142
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 2 cut(s) 592, 2452
MspI CCGG 3 cut(s) 56, 186, 227
MspR9I CCNGG 2 cut(s) 56, 1094
MunI CAATTG 2 cut(s) 1059, 1140
Mva1269I GAATGC 2 cut(s) 394, 1213
MvaI CCWGG 1 cut(s) 1094
MvnI CGCG 1 cut(s) 2531
MwoI GCNNNNNNNGC 5 cut(s) 223, 986, 1406, 2276, 2348
NciI CCSGG 1 cut(s) 56
NdeI CATATG 3 cut(s) 1053, 1177, 2317
NdeII GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
NlaIII CATG 6 cut(s) 63, 85, 641, 1451, 1666, 2140
NlaIV GGNNCC 7 cut(s) 205, 222, 1003, 1089, 1170, 2187, 2535
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 2625
NsiI ATGCAT 1 cut(s) 2142
NspI RCATGY 2 cut(s) 1451, 2140
NspV TTCGAA 2 cut(s) 386, 2412
PaeR7I CTCGAG 1 cut(s) 2650
PagI TCATGA 1 cut(s) 81
PceI AGGCCT 1 cut(s) 1945
PciI ACATGT 1 cut(s) 1447
PciSI GCTCTTC 1 cut(s) 1470
PctI GAATGC 2 cut(s) 394, 1213
PfeI GAWTC 5 cut(s) 237, 751, 1559, 1564, 2404
PkrI GCNGC 8 cut(s) 137, 274, 817, 991, 1805, 2089, 2378, 2454
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PscI ACATGT 1 cut(s) 1447
PshBI ATTAAT 1 cut(s) 515
Psp6I CCWGG 1 cut(s) 1092
PspFI CCCAGC 1 cut(s) 1702
PspGI CCWGG 1 cut(s) 1092
PspN4I GGNNCC 7 cut(s) 205, 222, 1003, 1089, 1170, 2187, 2535
PspPI GGNCC 2 cut(s) 203, 220
PspXI VCTCGAGB 1 cut(s) 2650
PstI CTGCAG 1 cut(s) 2092
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 2 cut(s) 1619, 2533
RsaI GTAC 7 cut(s) 588, 1334, 1446, 1529, 2033, 2234, 2528
RsaNI GTAC 7 cut(s) 587, 1333, 1445, 1528, 2032, 2233, 2527
RseI CAYNNNNRTG 1 cut(s) 642
SapI GCTCTTC 1 cut(s) 1470
SatI GCNGC 8 cut(s) 136, 273, 816, 990, 1804, 2088, 2377, 2453
Sau3AI GATC 8 cut(s) 1619, 1829, 1884, 2047, 2395, 2461, 2533, 2632
Sau96I GGNCC 2 cut(s) 203, 220
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 2 cut(s) 56, 1094
SduI GDGCHC 3 cut(s) 27, 471, 528
SfaNI GCATC 5 cut(s) 750, 1087, 1360, 1387, 2580
SfcI CTRYAG 1 cut(s) 2088
Sfr274I CTCGAG 1 cut(s) 2650
SfuI TTCGAA 2 cut(s) 386, 2412
SlaI CTCGAG 1 cut(s) 2650
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 1568, 2650
SmoI CTYRAG 2 cut(s) 1568, 2650
SseBI AGGCCT 1 cut(s) 1945
SspMI CTAG 3 cut(s) 1280, 1617, 1713
StuI AGGCCT 1 cut(s) 1945
StyD4I CCNGG 2 cut(s) 54, 1092
TaaI ACNGT 4 cut(s) 338, 1165, 2067, 2418
TaiI ACGT 5 cut(s) 927, 1517, 1650, 1682, 1739
TaqI TCGA 9 cut(s) 21, 295, 386, 1562, 1775, 1978, 2156, 2412, 2651
TatI WGTACW 5 cut(s) 586, 1332, 1527, 2031, 2232
TauI GCSGC 2 cut(s) 138, 2455
TfiI GAWTC 5 cut(s) 237, 751, 1559, 1564, 2404
TscAI CASTG 4 cut(s) 74, 1552, 1842, 2611
TseFI GTSAC 1 cut(s) 2625
TseI GCWGC 6 cut(s) 272, 815, 989, 1803, 2087, 2376
Tsp45I GTSAC 1 cut(s) 2625
TspGWI ACGGA 2 cut(s) 1612, 2376
TspRI CASTG 4 cut(s) 74, 1552, 1842, 2611
VspI ATTAAT 1 cut(s) 515
XapI RAATTY 9 cut(s) 712, 904, 1080, 1233, 1294, 1321, 1597, 2263, 2715
XceI RCATGY 2 cut(s) 1451, 2140
XhoI CTCGAG 1 cut(s) 2650
XmiI GTMKAC 4 cut(s) 601, 802, 1676, 2312
XspI CTAG 3 cut(s) 1280, 1617, 1713
Zsp2I ATGCAT 1 cut(s) 2142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.