Rh1CG323500

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000444
Physical Location & Seq
Forward (+)
59877339 .. 59881585
4247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG323500.1

Sequence Viewer

Length: 1812 bp
ATGGCTACAGGTTTGGTAATGGAGCCTGTGGCGATTACGTCACAGAAACATGACCCGGCTTGGAAACATTGCCAAATGTTTAAGAGTGGGGATAGAATTCAGCTCAAGTGTATATATTGTAGCAAGTTGTTTAGGGGTGGAGGGATTCATAGGATTAAAGAACACCTTGCAGGCCAGAAGGGCAATGCTTCCACTTGTTTGAGGGTTCCGCCGGATGTTCGGGCTCTAATGCAGCAGAGTTTAGATGGGGTTGTGGTTAAGAAGAGGAATAGGCAGAAGCTTGATGAGGAGATTACTAATATTACTCCCCCGCCGGATGGTGAGGTGGACTCACTTGGTGGGATTCAGAGTGATGTGAATAATGCGCTTGAGTTGACTGGAGTTCCACTTGAGACTAGTTCGAGGTTGTTGGTGAATCGAGAGGGGATAATGAGTGTTAGGAGGAAGAGGGGGAGAGGGAAAAGCTCTTCATCGTCCCATGGTGCTCATGGTGTCTGTAATAGTGGTGCATTGGTTTCTAGAAAAGTGAACAGTTATGTTCACGAGGCAATTGGGAGATTTTTGTTTGATATTGGAGCACATCCGGAGGCAGTGAACTCAGATTATTTCCAACCGATGATTGATGCAATTGCTTCGGGAGGTCAAGGGGTTGAGCCTCCCACGTATCATGATCTTCGGAGTTGGATATTGAAGAATTCGGTTGAGGAAGCGAGGAATAATATTGATAAACATAGGGCAACGTGGGGAAGGACTGGTTGCTCTATTTTGGTTGATCAGTGGAACACCGAGTTAGATAATGTTATGCTAAGTTTTCTGGTGTCCTGTCCCGAAGGAACTGTGTTTTTGGAATCTGTGGATGCATCAGCGATTATAAATTCCTCAGATGCTCTTTACGATTTGCTTAGGAGGGTGGTAGAATATGTTGGAGTAGGAGATGTAGTTCAAGTGATTACTAGCGGTGAAGAGCAATTTATAGTTGCAGGGAGAAGGTTGACTGATACATTCCCTACTCTGTATTGGATTCCATGTGCAGCTCGTTGTCTGGACTTGATACTTGAGGATTTTTGTAGCATTGAGTGGATACATGCAGTAATTGAGCAAGCTAGATCCATCACAAAGTTTGTGTACAATCACAATGTAGTCTTAAATATGGTTAGAAGGTCCACTTTTGGCAATGATATAGTAGAACCAGGAGTCACTCGCTCTGGTACAAACTTTACAACATTGAAACGACTGGCTGAGCTGAAACACTGTTTACAGGTCATGGTGACTTCGCAAGAGTGGATGGATTGCCCATATTCAAAGGAACCAGGGGGATTGGAGATGTCAGATCTTATAAGTGACAGGGATCAATCATTTTGGTCCTCATGCACCCTGGTTGTTCGTCTGACAAGTCCTCTCTTGCGAGTTTTGGGAATGGTTGGTTGTGAGAAGAGGCCTGCAATGGGTTTTATTTATGCTGGAATATATCGGGCAAAAGAAGCAATTAAGAAAGAACTTGTCAAGAGAGAGGAGTACATGGTGTACTGGAATATTATAGATCAACGATGGGAACAGCATTGGAATTTTCCTCTTCATACTGCAGGGTTCTACCTTAACCCTAAAATATTTTATAGCATTGAAGGAGATATCCATAATAGTATCCAGTCAGGAATGTATGATTGCATAGAGAGATTGGTTCCCGATATAAAAGTCCAAGATAAAATCATGAAAGAGATAATTTCGTACAAGAATGCTGCCGGGGATTTTCGGAGGAAGATGGCCATTCGGGCAAGAGACACTCTGCTTCCTGGTGAGAGCTCATTATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

603

Amino Acids

67.8

Weight (kDa)

7.22

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 220 - 371 2.2e-54 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 872, 1337
AccIII TCCGGA 1 cut(s) 583
AciI CCGC 3 cut(s) 209, 311, 957
AclWI GGATC 2 cut(s) 1101, 1356
AcoI YGGCCR 1 cut(s) 1761
AcsI RAATTY 4 cut(s) 96, 694, 874, 1564
AdeI CACNNNGTG 1 cut(s) 338
AfaI GTAC 5 cut(s) 1127, 1210, 1517, 1526, 1727
AfiI CCNNNNNNNGG 2 cut(s) 317, 1445
AgsI TTSAA 5 cut(s) 691, 944, 1228, 1302, 1622
AhlI ACTAGT 1 cut(s) 395
AjnI CCWGG 4 cut(s) 1189, 1309, 1374, 1789
AloI GAACNNNNNNTCC 2 cut(s) 924, 956
AluBI AGCT 7 cut(s) 103, 280, 465, 1034, 1103, 1243, 1800
AluI AGCT 7 cut(s) 103, 280, 465, 1034, 1103, 1243, 1800
Alw21I GWGCWC 3 cut(s) 487, 580, 1802
Alw26I GTCTC 2 cut(s) 386, 1770
AlwI GGATC 2 cut(s) 1101, 1356
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 3 cut(s) 172, 1436, 1761
ApeKI GCWGC 3 cut(s) 232, 1031, 1736
ApoI RAATTY 4 cut(s) 96, 694, 874, 1564
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 2 cut(s) 1161, 1362
AsuC2I CCSGG 2 cut(s) 56, 1741
AsuHPI GGTGA 5 cut(s) 332, 424, 971, 1279, 1805
AvaII GGWCC 2 cut(s) 1161, 1362
BalI TGGCCA 1 cut(s) 1763
BanII GRGCYC 2 cut(s) 226, 1802
BauI CACGAG 1 cut(s) 542
Bbv12I GWGCWC 3 cut(s) 487, 580, 1802
BbvI GCAGC 3 cut(s) 244, 1043, 1723
BccI CCATC 6 cut(s) 239, 311, 1118, 1279, 1542, 1753
BcgI CGANNNNNNTGC 2 cut(s) 615, 649
BciT130I CCWGG 4 cut(s) 1191, 1311, 1376, 1791
BciVI GTATCC 2 cut(s) 1074, 1652
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 2 cut(s) 56, 1741
BcoDI GTCTC 2 cut(s) 386, 1770
BcuI ACTAGT 1 cut(s) 395
BfaI CTAG 4 cut(s) 396, 519, 954, 1104
BfmI CTRYAG 2 cut(s) 6, 1581
BfuI GTATCC 2 cut(s) 1074, 1652
BglI GCCNNNNNGGC 2 cut(s) 180, 1769
BglII AGATCT 1 cut(s) 1330
BisI GCNGC 3 cut(s) 233, 1032, 1737
BlpI GCTNAGC 1 cut(s) 1239
BlsI GCNGC 3 cut(s) 234, 1033, 1738
Bme1390I CCNGG 6 cut(s) 56, 1191, 1311, 1376, 1741, 1791
Bme18I GGWCC 2 cut(s) 1161, 1362
BmgT120I GGNCC 2 cut(s) 1161, 1362
BmiI GGNNCC 4 cut(s) 24, 207, 1308, 1680
BmrFI CCNGG 6 cut(s) 56, 1191, 1311, 1376, 1741, 1791
BmsI GCATC 4 cut(s) 613, 847, 869, 874
BplI GAGNNNNNCTC 2 cut(s) 314, 346
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 902
Bpu1102I GCTNAGC 1 cut(s) 1239
BpuEI CTTGAG 4 cut(s) 89, 389, 410, 1076
BpuMI CCSGG 2 cut(s) 56, 1741
BsaAI YACGTR 1 cut(s) 663
BsaJI CCNNGG 4 cut(s) 478, 1310, 1374, 1740
BsaWI WCCGGW 1 cut(s) 583
BsaXI ACNNNNNCTCC 2 cut(s) 924, 954
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 1445
Bse1I ACTGG 5 cut(s) 382, 757, 1239, 1532, 1645
Bse3DI GCAATG 4 cut(s) 67, 190, 1180, 1449
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 4 cut(s) 1191, 1311, 1376, 1791
BseDI CCNNGG 4 cut(s) 478, 1310, 1374, 1740
BseGI GGATG 5 cut(s) 220, 322, 580, 862, 1290
BseLI CCNNNNNNNGG 2 cut(s) 317, 1445
BseMI GCAATG 4 cut(s) 67, 190, 1180, 1449
BseMII CTCAG 3 cut(s) 612, 894, 1230
BseNI ACTGG 5 cut(s) 382, 757, 1239, 1532, 1645
BseRI GAGGAG 2 cut(s) 302, 1526
BseXI GCAGC 3 cut(s) 244, 1043, 1723
BsgI GTGCAG 1 cut(s) 1050
BshFI GGCC 3 cut(s) 174, 1438, 1763
BsiHKAI GWGCWC 3 cut(s) 487, 580, 1802
BsiSI CCGG 5 cut(s) 56, 212, 314, 584, 1740
BslFI GGGAC 2 cut(s) 460, 810
BslI CCNNNNNNNGG 2 cut(s) 317, 1445
BsmAI GTCTC 2 cut(s) 386, 1770
BsmFI GGGAC 2 cut(s) 460, 810
BsmI GAATGC 1 cut(s) 1738
BsnI GGCC 3 cut(s) 174, 1438, 1763
Bsp1286I GDGCHC 4 cut(s) 226, 487, 580, 1802
Bsp13I TCCGGA 1 cut(s) 583
Bsp1407I TGTACA 1 cut(s) 1125
Bsp143I GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
Bsp1720I GCTNAGC 1 cut(s) 1239
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 3 cut(s) 209, 311, 957
BspANI GGCC 3 cut(s) 174, 1438, 1763
BspCNI CTCAG 3 cut(s) 611, 893, 1231
BspEI TCCGGA 1 cut(s) 583
BspHI TCATGA 2 cut(s) 667, 1707
BspLI GGNNCC 4 cut(s) 24, 207, 1308, 1680
BspMAI CTGCAG 1 cut(s) 1585
BspPI GGATC 2 cut(s) 1101, 1356
BspQI GCTCTTC 2 cut(s) 472, 957
BsrDI GCAATG 4 cut(s) 67, 190, 1180, 1449
BsrGI TGTACA 1 cut(s) 1125
BsrI ACTGG 5 cut(s) 382, 757, 1239, 1532, 1645
BssECI CCNNGG 4 cut(s) 478, 1310, 1374, 1740
BssMI GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 1 cut(s) 478
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 4 cut(s) 1191, 1311, 1376, 1791
Bst4CI ACNGT 3 cut(s) 533, 838, 1253
Bst6I CTCTTC 6 cut(s) 257, 440, 472, 957, 1427, 1578
BstAUI TGTACA 1 cut(s) 1125
BstBAI YACGTR 1 cut(s) 663
BstC8I GCNNGC 3 cut(s) 172, 1101, 1440
BstDEI CTNAG 5 cut(s) 598, 806, 880, 902, 1239
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 5 cut(s) 220, 322, 580, 862, 1290
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 6 cut(s) 673, 775, 1109, 1333, 1351, 1543
BstMAI GTCTC 2 cut(s) 386, 1770
BstMBI GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
BstMWI GCNNNNNNNGC 3 cut(s) 180, 1481, 1769
BstNI CCWGG 4 cut(s) 1191, 1311, 1376, 1791
BstNSI RCATGY 1 cut(s) 1088
BstSCI CCNGG 6 cut(s) 54, 1189, 1309, 1374, 1739, 1789
BstSFI CTRYAG 2 cut(s) 6, 1581
BstV1I GCAGC 3 cut(s) 244, 1043, 1723
BstX2I RGATCY 2 cut(s) 1106, 1330
BstYI RGATCY 2 cut(s) 1106, 1330
BsuI GTATCC 2 cut(s) 1074, 1652
BsuRI GGCC 3 cut(s) 174, 1438, 1763
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 5 cut(s) 220, 322, 580, 862, 1290
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 3 cut(s) 597, 782, 1249
Cac8I GCNNGC 3 cut(s) 172, 1101, 1440
CciI TCATGA 2 cut(s) 667, 1707
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 2 cut(s) 1161, 1362
Csp6I GTAC 5 cut(s) 1126, 1209, 1516, 1525, 1726
CviQI GTAC 5 cut(s) 1126, 1209, 1516, 1525, 1726
DdeI CTNAG 5 cut(s) 598, 806, 880, 902, 1239
DpnI GATC 6 cut(s) 672, 774, 1108, 1332, 1350, 1542
DpnII GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
DraIII CACNNNGTG 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 1761
Eam1104I CTCTTC 6 cut(s) 257, 440, 472, 957, 1427, 1578
EarI CTCTTC 6 cut(s) 257, 440, 472, 957, 1427, 1578
EciI GGCGGA 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 1800
Eco130I CCWWGG 1 cut(s) 478
Eco147I AGGCCT 1 cut(s) 1438
Eco24I GRGCYC 2 cut(s) 226, 1802
Eco32I GATATC 1 cut(s) 1630
Eco47I GGWCC 2 cut(s) 1161, 1362
Eco53kI GAGCTC 1 cut(s) 1800
EcoICRI GAGCTC 1 cut(s) 1800
EcoRI GAATTC 2 cut(s) 96, 694
EcoRII CCWGG 4 cut(s) 1189, 1309, 1374, 1789
EcoRV GATATC 1 cut(s) 1630
EcoT14I CCWWGG 1 cut(s) 478
EcoT22I ATGCAT 1 cut(s) 862
EcoT38I GRGCYC 2 cut(s) 226, 1802
ErhI CCWWGG 1 cut(s) 478
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 1150, 1182
FaqI GGGAC 2 cut(s) 460, 810
FauI CCCGC 1 cut(s) 318
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 3 cut(s) 233, 1032, 1737
FokI GGATG 5 cut(s) 227, 329, 567, 869, 1297
FriOI GRGCYC 2 cut(s) 226, 1802
Fsp4HI GCNGC 3 cut(s) 233, 1032, 1737
FspBI CTAG 4 cut(s) 396, 519, 954, 1104
GlaI GCGC 1 cut(s) 366
GluI GCNGC 3 cut(s) 233, 1032, 1737
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 174, 1438, 1763
HapII CCGG 5 cut(s) 56, 212, 314, 584, 1740
HhaI GCGC 1 cut(s) 367
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 2 cut(s) 375, 993
HindII GTYRAC 2 cut(s) 375, 993
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 7 cut(s) 145, 329, 343, 415, 848, 1021, 1194
HpaII CCGG 5 cut(s) 56, 212, 314, 584, 1740
HphI GGTGA 5 cut(s) 332, 424, 971, 1279, 1805
Hpy188I TCNGA 7 cut(s) 348, 601, 678, 883, 1330, 1389, 1752
HpyAV CCTTC 6 cut(s) 172, 741, 824, 981, 1152, 1616
HpyCH4III ACNGT 3 cut(s) 533, 838, 1253
HpyCH4IV ACGT 3 cut(s) 38, 662, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 1481, 1769
HpyF3I CTNAG 5 cut(s) 598, 806, 880, 902, 1239
HpySE526I ACGT 3 cut(s) 38, 662, 740
HspAI GCGC 1 cut(s) 365
Kpn2I TCCGGA 1 cut(s) 583
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
LguI GCTCTTC 2 cut(s) 472, 957
LmnI GCTCC 2 cut(s) 22, 575
Lsp1109I GCAGC 3 cut(s) 244, 1043, 1723
LweI GCATC 4 cut(s) 613, 847, 869, 874
MaeI CTAG 4 cut(s) 396, 519, 954, 1104
MaeII ACGT 3 cut(s) 38, 662, 740
MaeIII GTNAC 4 cut(s) 39, 1195, 1267, 1340
MalI GATC 6 cut(s) 672, 774, 1108, 1332, 1350, 1542
MboI GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
MboII GAAGA 9 cut(s) 274, 457, 459, 665, 703, 974, 1444, 1565, 1768
MfeI CAATTG 2 cut(s) 549, 627
MflI RGATCY 2 cut(s) 1106, 1330
MhlI GDGCHC 4 cut(s) 226, 487, 580, 1802
MlsI TGGCCA 1 cut(s) 1763
MluNI TGGCCA 1 cut(s) 1763
MlyI GAGTC 2 cut(s) 323, 1203
MmeI TCCRAC 3 cut(s) 634, 662, 904
Mox20I TGGCCA 1 cut(s) 1763
Mph1103I ATGCAT 1 cut(s) 862
MroI TCCGGA 1 cut(s) 583
MscI TGGCCA 1 cut(s) 1763
MseI TTAA 7 cut(s) 81, 156, 258, 1145, 1488, 1596, 1810
Msp20I TGGCCA 1 cut(s) 1763
MspI CCGG 5 cut(s) 56, 212, 314, 584, 1740
MspR9I CCNGG 6 cut(s) 56, 1191, 1311, 1376, 1741, 1791
MunI CAATTG 2 cut(s) 549, 627
Mva1269I GAATGC 1 cut(s) 1738
MvaI CCWGG 4 cut(s) 1191, 1311, 1376, 1791
MwoI GCNNNNNNNGC 3 cut(s) 180, 1481, 1769
NciI CCSGG 2 cut(s) 56, 1741
NcoI CCATGG 1 cut(s) 478
NdeII GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
NlaIV GGNNCC 4 cut(s) 24, 207, 1308, 1680
NmuCI GTSAC 4 cut(s) 39, 1195, 1267, 1340
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 1 cut(s) 1088
PagI TCATGA 2 cut(s) 667, 1707
PceI AGGCCT 1 cut(s) 1438
PciSI GCTCTTC 2 cut(s) 472, 957
PctI GAATGC 1 cut(s) 1738
PfeI GAWTC 5 cut(s) 145, 343, 415, 848, 1021
PkrI GCNGC 3 cut(s) 234, 1033, 1738
PleI GAGTC 2 cut(s) 323, 1202
PpsI GAGTC 2 cut(s) 323, 1202
Ppu21I YACGTR 1 cut(s) 663
PsiI TTATAA 2 cut(s) 872, 1337
Psp124BI GAGCTC 1 cut(s) 1802
Psp6I CCWGG 4 cut(s) 1189, 1309, 1374, 1789
PspGI CCWGG 4 cut(s) 1189, 1309, 1374, 1789
PspN4I GGNNCC 4 cut(s) 24, 207, 1308, 1680
PspPI GGNCC 2 cut(s) 1161, 1362
PstI CTGCAG 1 cut(s) 1585
PsuI RGATCY 2 cut(s) 1106, 1330
RsaI GTAC 5 cut(s) 1127, 1210, 1517, 1526, 1727
RsaNI GTAC 5 cut(s) 1126, 1209, 1516, 1525, 1726
SacI GAGCTC 1 cut(s) 1802
SapI GCTCTTC 2 cut(s) 472, 957
SaqAI TTAA 7 cut(s) 81, 156, 258, 1145, 1488, 1596, 1810
SatI GCNGC 3 cut(s) 233, 1032, 1737
Sau3AI GATC 6 cut(s) 670, 772, 1106, 1330, 1348, 1540
Sau96I GGNCC 2 cut(s) 1161, 1362
SchI GAGTC 2 cut(s) 323, 1203
ScrFI CCNGG 6 cut(s) 56, 1191, 1311, 1376, 1741, 1791
SduI GDGCHC 4 cut(s) 226, 487, 580, 1802
SfaNI GCATC 4 cut(s) 613, 847, 869, 874
SfcI CTRYAG 2 cut(s) 6, 1581
SinI GGWCC 2 cut(s) 1161, 1362
SmlI CTYRAG 4 cut(s) 104, 368, 389, 1055
SmoI CTYRAG 4 cut(s) 104, 368, 389, 1055
SpeI ACTAGT 1 cut(s) 395
SseBI AGGCCT 1 cut(s) 1438
SsiI CCGC 3 cut(s) 209, 311, 957
SspI AATATT 4 cut(s) 301, 721, 1534, 1608
SspMI CTAG 4 cut(s) 396, 519, 954, 1104
SstI GAGCTC 1 cut(s) 1802
StuI AGGCCT 1 cut(s) 1438
StyD4I CCNGG 6 cut(s) 54, 1189, 1309, 1374, 1739, 1789
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 3 cut(s) 533, 838, 1253
TaiI ACGT 3 cut(s) 41, 665, 743
TaqI TCGA 2 cut(s) 401, 418
TatI WGTACW 3 cut(s) 1125, 1515, 1524
TfiI GAWTC 5 cut(s) 145, 343, 415, 848, 1021
Tru1I TTAA 7 cut(s) 81, 156, 258, 1145, 1488, 1596, 1810
Tru9I TTAA 7 cut(s) 81, 156, 258, 1145, 1488, 1596, 1810
TscAI CASTG 3 cut(s) 597, 782, 1256
TseFI GTSAC 4 cut(s) 39, 1195, 1267, 1340
TseI GCWGC 3 cut(s) 232, 1031, 1736
Tsp45I GTSAC 4 cut(s) 39, 1195, 1267, 1340
TspDTI ATGAA 4 cut(s) 137, 459, 1565, 1724
TspRI CASTG 3 cut(s) 597, 782, 1256
VpaK11BI GGWCC 2 cut(s) 1161, 1362
XapI RAATTY 4 cut(s) 96, 694, 874, 1564
XbaI TCTAGA 1 cut(s) 518
XceI RCATGY 1 cut(s) 1088
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XspI CTAG 4 cut(s) 396, 519, 954, 1104
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.