FvH4_7g22891

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000444
Physical Location & Seq
Forward (+)
17987709 .. 17991263
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22891.t3

Sequence Viewer

Length: 2265 bp
ATGGAGCCTGTGCCCATTACGTCACAGAAACATGACCCGGCTTGGAAGCATTGCCAAATGTTTAAGAGTGGGGATAGAATTCAGCTCAAGTGTATATATTGTAGCAAGTTGTTTAGGGGTGGAGGGATTCATAGGATTAAGGAACACCTTGCAGGCCAAAAGGGCAATGCTTCAACTTGTTTGAGGGTCCCGCCGGATGTTCGGGGTTTAATGCAGCAGAGTTTGGATGGGGTGGTTGTGAAGAAAAGGAATAGGCAGAAGCTAGATGAGGAAATTACTAACATTACTCCCCCGCAGGATGGCGACGTGGACTCACTTGGTGGGACTCAGAGTGATGTGAATAATGCGGTTCAGTTGGTTGGAGTTTCGGTTGAGCCTATATCGAGGTTGTTGGTGAATCGAGAGGGGGTGACGAGTGTTAGGAGTATGGATAGGAGGAAGAGGGGGAGAGGGAAAAGCTCATGGTCGTCCCATGGTGTTCATGGTGTTTGTAATGGTGGTGCATTGGTTTCTAGGAAAGTGAATAGCTATGTTCATGAGGCGATTGGGAGGTTTTTGTTTGATATTGGGGCACCTCCGGAGGCAGTGAACTCGGCTTATTTTCAACCGATGATTGATGCAATTGCTTCAGGAGGTCCGGGGATGGAGCCTCCCACGTGTCATGATCTTCGGAGTTGGATATTGAAGAATTCGGTGGAGGAGGCGAGGAATAATATTGATAAACACAGGGCGACGTGGGGAAGGACTGGTTGCTCTATTTTGGTTGATCAGTGGAACACGGAGTTAGATAATGTTATGCTTAGTTTTCTGGTGTACTCTCCTGAAGGAACTGTGTTTTTGGAATCAGTGGACGCATCAGCGATTATAAATTCCTCAGATGCTCTTTATGATTTGCTTAGAAGAGTGGTAGAAGATGTTGGAGTAGGAGATGTAGTGCAAGTGATTACTAGCGGTGAAGAGCAATTTGTAGTTGCAGGCAGAAGGTTGGCTGATACTTTCCCTAATCTGTTTTGGATTCCATGTGCAGCTCGTTGTCTGGACCTGATCCTTGAGGATTTTGGTAGTCTTGATTGGATACATGCAGTAATTGAACAAGCTAGATCCATCACAAAGTTTGTATACAATCACAACGTAGTCTTAAATCTGGTTAGAAGGTCCACTTTTGGCAATGATATAGTGGAACCAGGAGTTACTCGCTTTGGTACAAGCTTTACAACATTGAAACGACTGGTTGATCTGAAACACTGTTTACAGGTCATGGTGACTTCACAAGAGTGGATGGATTGCCCATATTCAAAGGAACCAGGGGGATTGGAGATTTCAGATCTTATTAGCGACAGGGATCAATCATTTTGGTCCTCATGCACCCTGATTGTTCGTTTGACAAGTCCTCTGTTGCGAGTTTTGAGAATGGTTGGTTGTGAGAAGAGGCCTGCAATGGGTTTTATTTATGCTGGAATGTATCGAGCAAAAGAAGCAATTAAGAAGGAACTTGTTAAGAGAGAGGAATACATGGTGTACTGGAATATTATAGATCAAAGATGGGAACAGCATTGGAATTTTCCTCTTCATGCTGCAGGGTTCTACCTTAACCCCAAAATCTTTTATAGTATTGAAGGAGATATTCATAATAGTATCCAATCGGGTATGTATGACTGCATAGAGAGAATGGTTCCTGATATAAAAGTCCAAGATAAAATCATGAAAGAGATAATTTCGTACAAGAATGCTGCTGGGGATTTTCGAAGGAAGATGGCCATTCGGGCTAGAGATACTCTGCTTCCTGCTGAATGGTGGTCAACATATGGTGGAGGTTGCCCGAATCTGGCTCGCTTGGCAATTCGCATTCTGAGTCAAACTTGTGGTTCAATTGGGTATAGGCAAAGTCAGATTCCTTTTGAGAAAGCACATGGCATAAGGAACTGCCTAGAGCGCCAACGTCTCAGGGACCTTGTCTTTGTTCAGTACAACCTACGACTGAGGCAAATGGTTGATAAAAACAATGGAGAAGATTGTATGGATCCTATATCATTTGACAGCATTAGCTTAGTTGAAGACTGGGTTACAGGGAAGGACGTGTGCTCGGAAGACTTTGAAGGTTCAAGTTGGATGTCACTTGACTCACCCTCTGCTAGCACGATGCTTTTAGGGCCCTCAAATGATGATGCCGAGGATTTGGGCTCTGGGTTTTATGATGGTGAGATTTTCAGTAGGGGAAAAGACGGGGAAATAGAAATTCTCGAAGACAATGTAGAAAATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

755

Amino Acids

84.84

Weight (kDa)

5.93

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 224 - 375 1.5e-54 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 591 - 664 5.4e-06 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 571
AccI GTMKAC 1 cut(s) 1119
AccIII TCCGGA 1 cut(s) 577
AciI CCGC 4 cut(s) 191, 293, 347, 951
AclWI GGATC 5 cut(s) 1039, 1095, 1350, 2015, 2028
AcoI YGGCCR 1 cut(s) 1755
AcsI RAATTY 5 cut(s) 78, 688, 868, 1558, 2235
AcuI CTGAAG 2 cut(s) 612, 843
AcvI CACGTG 1 cut(s) 657
AdeI CACNNNGTG 1 cut(s) 320
AfaI GTAC 5 cut(s) 815, 1204, 1520, 1721, 1967
AfiI CCNNNNNNNGG 3 cut(s) 299, 1439, 1825
AflIII ACRYGT 2 cut(s) 656, 2076
AjiI CACGTC 3 cut(s) 307, 735, 2077
AjnI CCWGG 2 cut(s) 1183, 1303
AleI CACNNNNGTG 1 cut(s) 1273
AluBI AGCT 8 cut(s) 85, 262, 459, 528, 1028, 1097, 1209, 2046
AluI AGCT 8 cut(s) 85, 262, 459, 528, 1028, 1097, 1209, 2046
Alw21I GWGCWC 1 cut(s) 2084
Alw26I GTCTC 1 cut(s) 1946
AlwI GGATC 5 cut(s) 1039, 1095, 1350, 2015, 2028
Aor13HI TCCGGA 1 cut(s) 577
AoxI GGCC 4 cut(s) 154, 1430, 1755, 2150
ApaI GGGCCC 1 cut(s) 2154
ApeKI GCWGC 4 cut(s) 214, 1025, 1574, 1730
ApoI RAATTY 5 cut(s) 78, 688, 868, 1558, 2235
ArsI GACNNNNNNTTYG 2 cut(s) 1940, 1972
AspLEI GCGC 1 cut(s) 1935
AspS9I GGNCC 8 cut(s) 187, 635, 1039, 1155, 1356, 1948, 2150, 2151
AsuC2I CCSGG 2 cut(s) 38, 639
AsuHPI GGTGA 6 cut(s) 406, 421, 965, 1273, 2115, 2210
AsuII TTCGAA 1 cut(s) 1744
AsuNHI GCTAGC 1 cut(s) 2132
AvaII GGWCC 6 cut(s) 187, 635, 1039, 1155, 1356, 1948
BaeGI GKGCMC 3 cut(s) 15, 574, 2154
BalI TGGCCA 1 cut(s) 1757
BamHI GGATCC 1 cut(s) 2020
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 2 cut(s) 2154, 2183
BbrPI CACGTG 1 cut(s) 657
BbsI GAAGAC 3 cut(s) 2061, 2094, 2250
Bbv12I GWGCWC 1 cut(s) 2084
BbvI GCAGC 4 cut(s) 226, 1037, 1561, 1717
BccI CCATC 8 cut(s) 221, 293, 637, 1112, 1273, 1536, 1747, 2189
BcgI CGANNNNNNTGC 2 cut(s) 573, 607
BciT130I CCWGG 2 cut(s) 1185, 1305
BciVI GTATCC 2 cut(s) 1068, 1646
BclI TGATCA 1 cut(s) 766
BcnI CCSGG 2 cut(s) 38, 639
BcoDI GTCTC 1 cut(s) 1946
BfaI CTAG 8 cut(s) 263, 513, 948, 1098, 1767, 1928, 2133, 2263
BfmI CTRYAG 1 cut(s) 1575
BfoI RGCGCY 1 cut(s) 1936
BfuI GTATCC 2 cut(s) 1068, 1646
BglI GCCNNNNNGGC 2 cut(s) 162, 1763
BglII AGATCT 1 cut(s) 1324
BisI GCNGC 4 cut(s) 215, 1026, 1575, 1731
BlsI GCNGC 4 cut(s) 216, 1027, 1576, 1732
Bme1390I CCNGG 4 cut(s) 38, 639, 1185, 1305
Bme18I GGWCC 6 cut(s) 187, 635, 1039, 1155, 1356, 1948
BmgBI CACGTC 3 cut(s) 307, 735, 2077
BmgT120I GGNCC 8 cut(s) 187, 635, 1039, 1155, 1356, 1948, 2150, 2151
BmrFI CCNGG 4 cut(s) 38, 639, 1185, 1305
BmrI ACTGGG 1 cut(s) 2068
BmsI GCATC 5 cut(s) 607, 863, 868, 2130, 2155
BmtI GCTAGC 1 cut(s) 2136
BmuI ACTGGG 1 cut(s) 2068
BpiI GAAGAC 3 cut(s) 2061, 2094, 2250
Bpu14I TTCGAA 1 cut(s) 1744
BpuEI CTTGAG 2 cut(s) 71, 1070
BpuMI CCSGG 2 cut(s) 38, 639
BsaAI YACGTR 1 cut(s) 657
BsaJI CCNNGG 4 cut(s) 472, 638, 1304, 2169
BsaWI WCCGGW 1 cut(s) 577
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 918, 948
Bsc4I CCNNNNNNNGG 3 cut(s) 299, 1439, 1825
Bse1I ACTGG 4 cut(s) 751, 1233, 1526, 2063
Bse3DI GCAATG 4 cut(s) 49, 172, 1174, 1443
BseAI TCCGGA 1 cut(s) 577
BseBI CCWGG 2 cut(s) 1185, 1305
BseDI CCNNGG 4 cut(s) 472, 638, 1304, 2169
BseGI GGATG 6 cut(s) 202, 232, 304, 648, 1284, 2115
BseLI CCNNNNNNNGG 3 cut(s) 299, 1439, 1825
BseMI GCAATG 4 cut(s) 49, 172, 1174, 1443
BseMII CTCAG 5 cut(s) 341, 888, 1841, 1957, 1970
BseNI ACTGG 4 cut(s) 751, 1233, 1526, 2063
BseRI GAGGAG 1 cut(s) 713
BseSI GKGCMC 3 cut(s) 15, 574, 2154
BseXI GCAGC 4 cut(s) 226, 1037, 1561, 1717
BseYI CCCAGC 1 cut(s) 1733
BsgI GTGCAG 1 cut(s) 1044
BshFI GGCC 4 cut(s) 156, 1432, 1757, 2152
BshNI GGYRCC 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 2084
BsiSI CCGG 4 cut(s) 38, 194, 578, 638
BslFI GGGAC 4 cut(s) 173, 337, 454, 1961
BslI CCNNNNNNNGG 3 cut(s) 299, 1439, 1825
BsmAI GTCTC 1 cut(s) 1946
BsmBI CGTCTC 1 cut(s) 1946
BsmFI GGGAC 4 cut(s) 173, 337, 454, 1961
BsmI GAATGC 2 cut(s) 1732, 1845
BsnI GGCC 4 cut(s) 156, 1432, 1757, 2152
Bsp119I TTCGAA 1 cut(s) 1744
Bsp120I GGGCCC 1 cut(s) 2150
Bsp1286I GDGCHC 5 cut(s) 15, 574, 2084, 2154, 2183
Bsp13I TCCGGA 1 cut(s) 577
Bsp143I GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 4 cut(s) 191, 293, 347, 951
BspANI GGCC 4 cut(s) 156, 1432, 1757, 2152
BspCNI CTCAG 5 cut(s) 340, 887, 1842, 1956, 1971
BspEI TCCGGA 1 cut(s) 577
BspHI TCATGA 3 cut(s) 535, 661, 1701
BspMAI CTGCAG 1 cut(s) 1579
BspOI GCTAGC 1 cut(s) 2136
BspPI GGATC 5 cut(s) 1039, 1095, 1350, 2015, 2028
BspQI GCTCTTC 1 cut(s) 951
BspT104I TTCGAA 1 cut(s) 1744
BspT107I GGYRCC 1 cut(s) 571
BsrDI GCAATG 4 cut(s) 49, 172, 1174, 1443
BsrI ACTGG 4 cut(s) 751, 1233, 1526, 2063
BssECI CCNNGG 4 cut(s) 472, 638, 1304, 2169
BssMI GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
BssNAI GTATAC 1 cut(s) 1120
BssT1I CCWWGG 1 cut(s) 472
Bst1107I GTATAC 1 cut(s) 1120
Bst2UI CCWGG 2 cut(s) 1185, 1305
Bst4CI ACNGT 2 cut(s) 832, 1247
Bst6I CTCTTC 5 cut(s) 434, 895, 951, 1421, 1572
BstBAI YACGTR 1 cut(s) 657
BstBI TTCGAA 1 cut(s) 1744
BstC8I GCNNGC 5 cut(s) 154, 976, 1434, 1831, 2134
BstDEI CTNAG 8 cut(s) 327, 800, 874, 896, 1850, 1943, 1979, 2047
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 6 cut(s) 202, 232, 304, 648, 1284, 2115
BstH2I RGCGCY 1 cut(s) 1936
BstHHI GCGC 1 cut(s) 1935
BstKTI GATC 9 cut(s) 667, 769, 1047, 1103, 1237, 1327, 1345, 1537, 2023
BstMAI GTCTC 1 cut(s) 1946
BstMBI GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
BstMWI GCNNNNNNNGC 6 cut(s) 162, 1475, 1763, 1835, 1932, 2149
BstNI CCWGG 2 cut(s) 1185, 1305
BstNSI RCATGY 1 cut(s) 1082
BstSCI CCNGG 4 cut(s) 36, 637, 1183, 1303
BstSFI CTRYAG 1 cut(s) 1575
BstSLI GKGCMC 3 cut(s) 15, 574, 2154
BstV1I GCAGC 4 cut(s) 226, 1037, 1561, 1717
BstV2I GAAGAC 3 cut(s) 2061, 2094, 2250
BstX2I RGATCY 3 cut(s) 1100, 1324, 2020
BstYI RGATCY 3 cut(s) 1100, 1324, 2020
BstZ17I GTATAC 1 cut(s) 1120
BsuI GTATCC 2 cut(s) 1068, 1646
BsuRI GGCC 4 cut(s) 156, 1432, 1757, 2152
BtgI CCRYGG 1 cut(s) 472
BtrI CACGTC 3 cut(s) 307, 735, 2077
BtsCI GGATG 6 cut(s) 202, 232, 304, 648, 1284, 2115
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 4 cut(s) 591, 776, 852, 1243
Cac8I GCNNGC 5 cut(s) 154, 976, 1434, 1831, 2134
CciI TCATGA 3 cut(s) 535, 661, 1701
CfoI GCGC 1 cut(s) 1935
Cfr13I GGNCC 8 cut(s) 187, 635, 1039, 1155, 1356, 1948, 2150, 2151
CseI GACGC 1 cut(s) 860
Csp6I GTAC 5 cut(s) 814, 1203, 1519, 1720, 1966
CviQI GTAC 5 cut(s) 814, 1203, 1519, 1720, 1966
DdeI CTNAG 8 cut(s) 327, 800, 874, 896, 1850, 1943, 1979, 2047
DpnI GATC 9 cut(s) 666, 768, 1046, 1102, 1236, 1326, 1344, 1536, 2022
DpnII GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
DraIII CACNNNGTG 1 cut(s) 320
EaeI YGGCCR 1 cut(s) 1755
Eam1104I CTCTTC 5 cut(s) 434, 895, 951, 1421, 1572
EarI CTCTTC 5 cut(s) 434, 895, 951, 1421, 1572
Eco130I CCWWGG 1 cut(s) 472
Eco147I AGGCCT 1 cut(s) 1432
Eco24I GRGCYC 2 cut(s) 2154, 2183
Eco47I GGWCC 6 cut(s) 187, 635, 1039, 1155, 1356, 1948
Eco57I CTGAAG 2 cut(s) 612, 843
Eco72I CACGTG 1 cut(s) 657
EcoO109I RGGNCCY 4 cut(s) 187, 1948, 2150, 2151
EcoRI GAATTC 2 cut(s) 78, 688
EcoRII CCWGG 2 cut(s) 1183, 1303
EcoT14I CCWWGG 1 cut(s) 472
EcoT38I GRGCYC 2 cut(s) 2154, 2183
ErhI CCWWGG 1 cut(s) 472
Esp3I CGTCTC 1 cut(s) 1946
FalI AAGNNNNNCTT 2 cut(s) 1144, 1176
FaqI GGGAC 4 cut(s) 173, 337, 454, 1961
FauI CCCGC 2 cut(s) 198, 300
FauNDI CATATG 1 cut(s) 1804
FbaI TGATCA 1 cut(s) 766
FblI GTMKAC 1 cut(s) 1119
Fnu4HI GCNGC 4 cut(s) 215, 1026, 1575, 1731
FokI GGATG 6 cut(s) 209, 239, 311, 655, 1291, 2122
FriOI GRGCYC 2 cut(s) 2154, 2183
Fsp4HI GCNGC 4 cut(s) 215, 1026, 1575, 1731
FspBI CTAG 8 cut(s) 263, 513, 948, 1098, 1767, 1928, 2133, 2263
GlaI GCGC 1 cut(s) 1934
GluI GCNGC 4 cut(s) 215, 1026, 1575, 1731
GsaI CCCAGC 1 cut(s) 1737
HaeII RGCGCY 1 cut(s) 1936
HaeIII GGCC 4 cut(s) 156, 1432, 1757, 2152
HapII CCGG 4 cut(s) 38, 194, 578, 638
HgaI GACGC 1 cut(s) 860
HhaI GCGC 1 cut(s) 1935
Hin6I GCGC 1 cut(s) 1933
HinP1I GCGC 1 cut(s) 1933
HincII GTYRAC 1 cut(s) 1800
HindII GTYRAC 1 cut(s) 1800
HindIII AAGCTT 1 cut(s) 1207
HpaII CCGG 4 cut(s) 38, 194, 578, 638
HphI GGTGA 6 cut(s) 406, 421, 965, 1273, 2115, 2210
Hpy166II GTNNAC 9 cut(s) 310, 589, 814, 850, 1120, 1158, 1250, 1519, 1800
Hpy188I TCNGA 8 cut(s) 330, 672, 877, 1239, 1324, 1851, 1890, 2086
Hpy8I GTNNAC 9 cut(s) 310, 589, 814, 850, 1120, 1158, 1250, 1519, 1800
Hpy99I CGWCG 2 cut(s) 308, 736
HpyAV CCTTC 9 cut(s) 735, 818, 975, 1146, 1480, 1610, 1740, 2065, 2090
HpyCH4III ACNGT 2 cut(s) 832, 1247
HpyCH4IV ACGT 7 cut(s) 20, 306, 656, 734, 1131, 1939, 2076
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 1475, 1763, 1835, 1932, 2149
HpyF3I CTNAG 8 cut(s) 327, 800, 874, 896, 1850, 1943, 1979, 2047
HpySE526I ACGT 7 cut(s) 20, 306, 656, 734, 1131, 1939, 2076
HspAI GCGC 1 cut(s) 1933
KflI GGGWCCC 1 cut(s) 187
Kpn2I TCCGGA 1 cut(s) 577
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
LguI GCTCTTC 1 cut(s) 951
LmnI GCTCC 2 cut(s) 4, 646
Lsp1109I GCAGC 4 cut(s) 226, 1037, 1561, 1717
LweI GCATC 5 cut(s) 607, 863, 868, 2130, 2155
MaeI CTAG 8 cut(s) 263, 513, 948, 1098, 1767, 1928, 2133, 2263
MaeII ACGT 7 cut(s) 20, 306, 656, 734, 1131, 1939, 2076
MaeIII GTNAC 6 cut(s) 21, 409, 1189, 1261, 2062, 2112
MalI GATC 9 cut(s) 666, 768, 1046, 1102, 1236, 1326, 1344, 1536, 2022
MboI GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
MfeI CAATTG 2 cut(s) 621, 1869
MflI RGATCY 3 cut(s) 1100, 1324, 2020
MhlI GDGCHC 5 cut(s) 15, 574, 2084, 2154, 2183
MlsI TGGCCA 1 cut(s) 1757
MluNI TGGCCA 1 cut(s) 1757
MlyI GAGTC 4 cut(s) 305, 319, 1861, 2114
MmeI TCCRAC 4 cut(s) 340, 656, 898, 2087
Mox20I TGGCCA 1 cut(s) 1757
MroI TCCGGA 1 cut(s) 577
MscI TGGCCA 1 cut(s) 1757
MseI TTAA 7 cut(s) 63, 138, 209, 1139, 1482, 1497, 1590
MslI CAYNNNNRTG 1 cut(s) 1273
Msp20I TGGCCA 1 cut(s) 1757
MspI CCGG 4 cut(s) 38, 194, 578, 638
MspR9I CCNGG 4 cut(s) 38, 639, 1185, 1305
MunI CAATTG 2 cut(s) 621, 1869
Mva1269I GAATGC 2 cut(s) 1732, 1845
MvaI CCWGG 2 cut(s) 1185, 1305
MwoI GCNNNNNNNGC 6 cut(s) 162, 1475, 1763, 1835, 1932, 2149
NciI CCSGG 2 cut(s) 38, 639
NcoI CCATGG 1 cut(s) 472
NdeI CATATG 1 cut(s) 1804
NdeII GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
NheI GCTAGC 1 cut(s) 2132
NmeAIII GCCGAG 2 cut(s) 572, 2194
NmuCI GTSAC 4 cut(s) 21, 409, 1261, 2112
NspI RCATGY 1 cut(s) 1082
NspV TTCGAA 1 cut(s) 1744
OliI CACNNNNGTG 1 cut(s) 1273
PagI TCATGA 3 cut(s) 535, 661, 1701
PceI AGGCCT 1 cut(s) 1432
PciSI GCTCTTC 1 cut(s) 951
PctI GAATGC 2 cut(s) 1732, 1845
PfeI GAWTC 6 cut(s) 127, 397, 842, 1015, 1822, 1891
PflFI GACNNNGTC 1 cut(s) 1952
PkrI GCNGC 4 cut(s) 216, 1027, 1576, 1732
PleI GAGTC 4 cut(s) 305, 319, 1860, 2114
PmaCI CACGTG 1 cut(s) 657
PmlI CACGTG 1 cut(s) 657
PpsI GAGTC 4 cut(s) 305, 319, 1860, 2114
Ppu21I YACGTR 1 cut(s) 657
PpuMI RGGWCCY 2 cut(s) 187, 1948
PsiI TTATAA 1 cut(s) 866
Psp5II RGGWCCY 2 cut(s) 187, 1948
Psp6I CCWGG 2 cut(s) 1183, 1303
PspCI CACGTG 1 cut(s) 657
PspFI CCCAGC 1 cut(s) 1733
PspGI CCWGG 2 cut(s) 1183, 1303
PspOMI GGGCCC 1 cut(s) 2150
PspPI GGNCC 8 cut(s) 187, 635, 1039, 1155, 1356, 1948, 2150, 2151
PspPPI RGGWCCY 2 cut(s) 187, 1948
PstI CTGCAG 1 cut(s) 1579
PsuI RGATCY 3 cut(s) 1100, 1324, 2020
PsyI GACNNNGTC 1 cut(s) 1952
RsaI GTAC 5 cut(s) 815, 1204, 1520, 1721, 1967
RsaNI GTAC 5 cut(s) 814, 1203, 1519, 1720, 1966
RseI CAYNNNNRTG 1 cut(s) 1273
SapI GCTCTTC 1 cut(s) 951
SaqAI TTAA 7 cut(s) 63, 138, 209, 1139, 1482, 1497, 1590
SatI GCNGC 4 cut(s) 215, 1026, 1575, 1731
Sau3AI GATC 9 cut(s) 664, 766, 1044, 1100, 1234, 1324, 1342, 1534, 2020
Sau96I GGNCC 8 cut(s) 187, 635, 1039, 1155, 1356, 1948, 2150, 2151
SchI GAGTC 4 cut(s) 305, 319, 1861, 2114
ScrFI CCNGG 4 cut(s) 38, 639, 1185, 1305
SduI GDGCHC 5 cut(s) 15, 574, 2084, 2154, 2183
SfaNI GCATC 5 cut(s) 607, 863, 868, 2130, 2155
SfcI CTRYAG 1 cut(s) 1575
SfuI TTCGAA 1 cut(s) 1744
SinI GGWCC 6 cut(s) 187, 635, 1039, 1155, 1356, 1948
SmiMI CAYNNNNRTG 1 cut(s) 1273
SmlI CTYRAG 2 cut(s) 86, 1049
SmoI CTYRAG 2 cut(s) 86, 1049
SseBI AGGCCT 1 cut(s) 1432
SsiI CCGC 4 cut(s) 191, 293, 347, 951
SspI AATATT 2 cut(s) 715, 1528
SspMI CTAG 8 cut(s) 263, 513, 948, 1098, 1767, 1928, 2133, 2263
StuI AGGCCT 1 cut(s) 1432
StyD4I CCNGG 4 cut(s) 36, 637, 1183, 1303
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 2 cut(s) 832, 1247
TaiI ACGT 7 cut(s) 23, 309, 659, 737, 1134, 1942, 2079
TaqI TCGA 5 cut(s) 383, 400, 1465, 1744, 2241
TatI WGTACW 3 cut(s) 813, 1518, 1965
TfiI GAWTC 6 cut(s) 127, 397, 842, 1015, 1822, 1891
Tru1I TTAA 7 cut(s) 63, 138, 209, 1139, 1482, 1497, 1590
Tru9I TTAA 7 cut(s) 63, 138, 209, 1139, 1482, 1497, 1590
TscAI CASTG 4 cut(s) 591, 776, 852, 1250
TseFI GTSAC 4 cut(s) 21, 409, 1261, 2112
TseI GCWGC 4 cut(s) 214, 1025, 1574, 1730
Tsp45I GTSAC 4 cut(s) 21, 409, 1261, 2112
TspDTI ATGAA 6 cut(s) 119, 470, 524, 1559, 1616, 1718
TspGWI ACGGA 1 cut(s) 794
TspRI CASTG 4 cut(s) 591, 776, 852, 1250
Tth111I GACNNNGTC 1 cut(s) 1952
VpaK11BI GGWCC 6 cut(s) 187, 635, 1039, 1155, 1356, 1948
XapI RAATTY 5 cut(s) 78, 688, 868, 1558, 2235
XceI RCATGY 1 cut(s) 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XmiI GTMKAC 1 cut(s) 1119
XspI CTAG 8 cut(s) 263, 513, 948, 1098, 1767, 1928, 2133, 2263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.