RchiOBHm_Chr1g0368051

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000444
Physical Location & Seq
Forward (+)
58283338 .. 58286932
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59242

Sequence Viewer

Length: 2265 bp
ATGGAGCCTGTGGCGATTACGTCACAGAAACATGACCCGGCTTGGAAACACTGCCAAATGTTTAAGAGTGGGGATAGAATTCAGCTCAAGTGTATATATTGTAGCAAGTTGTTTAGGGGTGGAGGGATTCATAGGATTAAGGAACACCTTGCAGGCCAGAAGGGCAATGCTTCCACTTGTTTGAGGGTTCCGCCGGATGTTCGGGCTTTAATGCAGCAGAGTTTAGATGGGGTTGTGGTTAAGAAGAGGAATAGGCAGAAGCTTGATGAGGAGATTACTAACATTACTCCCCCGCCGGATGGTGAGGTGGACTCACTTGGTGGGATTCAGAGTGATGTCAATAATGCGCTTGAGTTGACTGGAGTTCCACTTGAGACTAGTTCGAGGTTGTTGGTGAATCGAGAGGGGATAATGAGTGTTAGGAGTATGGATAGGAGGAAGAGGGGGAGAGGGAAAAGCTCTTCATCGTCCCATGGTGCTCATGGTGTCTGTAATAGTGGTGCATTGGTTTCTAGAAAAGTGAACAGTTATGTTCACGAGGCAATTGGGAGATTTTTGTTTGATATTGGGGCACCTCCGGAGGCATTGAACTCAGATTATCTCCAACCAATGATTGATGCAATTGCTTCGGGAGGTCAAGGGGTTGAGCCTCCCACGTATCATGATCTTCGGAGTTGGATATTGAAGAATTCGGTTGAGGAAGCGAGGAATAATATTGATAAACATAGGGCAACGTGGGGAAGGACTGGTTGCTCTATTTTGGTTGATCAGTGGAACACCGAGTTAGATAATGTTATGCTAAGTTTTCTGGTGTACTGTCCCGAAGGAACTGTGTTTTTGGAATCTATGGATGCATCAGCGATTATAAATTCCTCAGATGCTCTTTACGATTTGCTTAGGAGAGTGGTAGAATATGTTGGAGTAGGAGATGTAGTTCAAGTGATTACTAGCTGTGAAGAGCAATTTATAGTTGCAGGGAGAAGGTTGACTGATACATTCCCTACTCTGTATTGGATTCCATGTGCAGCTCGTTGTCTGGACTTGATACTTGAGGATTTTTGTAGCATTGAGTGGATACATGCAGTAATTGAGCAAGCTAGATCCATCACAAAGTTTGTGTACAATAACAATGTAGTTTTAAATATGGTTAGAAGGTCCACTTTTGGCAATGATATAGTAGAACCAGGAGTCACTCGCTTTGGTACAAACTTTACAACATTGAAACGACTGGCTGAGCTGAAACACTGTTTACAGGTCATGGTGACTTCGCAAGAGTGGATGGAGTGCCCATATTCAAAGGAACCAGGGGGATTGGAGATGTCAGATCTTATAAGTGACAGGGATCAATCATTTTGGTCCTCATGCACCCTGGTTGTTCGTCTGACAGGTCCTCTCTTGCGAGTTTTGAGAATGGTTGGTTGTGAGAAGAGGCCTGCAATGGGTTTTATTTATGCTGGAATGTATCGGGCAAAAGAAGCAATTAAGAAAGAACTTGTCAAGAGAGAGGAGTACATGGTGTACTGGAATATTATAGATCAACGATGGGAACAGCATTGGAATTTTCCTCTTCATACTGCAGGGTTCTACCTTAACCCTAAAATATTTTATAGCATTGAAGGAGATATCCATAATAGTATCCAGTCAGGAATGTATGATTGCATAGAGAGATTGGTTCCCGATATAAAAGTCCAAGATAAAATCATGAAAGAGATAATTTCATACAAGAATGCTGCCGGGGATTTTCGGAGGAAGATGGCCATTCGGGCAAGAGACACTCTGCTTCCTGCTGAATGGTGGTCAACATACGGTGGCGGTTGCCCAAATCTAGCTCGCTTGGCAATTCGCGTTCTCAGTCAAACTTGTGGTTCAATTGGGTATAGGCAAAGTCAGATTCCTTTTGAGAAAGCACACAGCATAAGGAACTGCCTAGAGCGCCAACGTCTCAGGGACCTTGTCTTTGTTCAGTACAACCTACGACTCAGACAAATGGTTGATAAAAACATTGGAGAAGATTGTATGGATCCTATATCATTTGCAAGCATTAGCATTGTTGAAGACTGGGTAACAGGGAAAGACGTGTGCTCGGAAGACCTTGAGAGTTCAAGTTGGATGGCACTTGACTCACCCTCTGCTAGCACGATGCTTTTAGGACCCTCAAATGATGATGCCGAGGATTTGGGCTCTGGGTTTTATGATGGTGAGATTTTCAGTAGGGGGAAAGACGGGGAAAATGAAATTGTTGAAGACAATGTAGAAAATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

754

Amino Acids

85.07

Weight (kDa)

5.9

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 218 - 369 1.8e-54 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 585 - 658 2e-06 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 866, 1331
AccB1I GGYRCC 1 cut(s) 571
AccII CGCG 1 cut(s) 1845
AccIII TCCGGA 1 cut(s) 577
AciI CCGC 3 cut(s) 191, 293, 1812
AclWI GGATC 4 cut(s) 1095, 1350, 2015, 2028
AcoI YGGCCR 1 cut(s) 1755
AcsI RAATTY 4 cut(s) 78, 688, 868, 1558
AdeI CACNNNGTG 1 cut(s) 320
AfaI GTAC 6 cut(s) 815, 1121, 1204, 1511, 1520, 1967
AfiI CCNNNNNNNGG 2 cut(s) 299, 1439
AflIII ACRYGT 1 cut(s) 2076
AhlI ACTAGT 1 cut(s) 377
AjiI CACGTC 1 cut(s) 2077
AjnI CCWGG 3 cut(s) 1183, 1303, 1368
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
AluBI AGCT 8 cut(s) 85, 262, 459, 951, 1028, 1097, 1237, 1829
AluI AGCT 8 cut(s) 85, 262, 459, 951, 1028, 1097, 1237, 1829
Alw21I GWGCWC 2 cut(s) 481, 2084
Alw26I GTCTC 3 cut(s) 368, 1764, 1946
AlwI GGATC 4 cut(s) 1095, 1350, 2015, 2028
Aor13HI TCCGGA 1 cut(s) 577
AoxI GGCC 3 cut(s) 154, 1430, 1755
ApeKI GCWGC 3 cut(s) 214, 1025, 1730
ApoI RAATTY 4 cut(s) 78, 688, 868, 1558
ArsI GACNNNNNNTTYG 2 cut(s) 1940, 1972
AspLEI GCGC 2 cut(s) 349, 1935
AspS9I GGNCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
AsuC2I CCSGG 2 cut(s) 38, 1735
AsuHPI GGTGA 5 cut(s) 314, 406, 1273, 2115, 2210
AsuNHI GCTAGC 1 cut(s) 2132
AvaII GGWCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
BaeGI GKGCMC 2 cut(s) 574, 1289
BalI TGGCCA 1 cut(s) 1757
BamHI GGATCC 1 cut(s) 2020
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 2183
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 3 cut(s) 2061, 2094, 2250
Bbv12I GWGCWC 2 cut(s) 481, 2084
BbvI GCAGC 3 cut(s) 226, 1037, 1717
BccI CCATC 8 cut(s) 221, 293, 1112, 1273, 1536, 1747, 2104, 2189
BcgI CGANNNNNNTGC 2 cut(s) 609, 643
BciT130I CCWGG 3 cut(s) 1185, 1305, 1370
BciVI GTATCC 2 cut(s) 1068, 1646
BclI TGATCA 1 cut(s) 766
BcnI CCSGG 2 cut(s) 38, 1735
BcoDI GTCTC 3 cut(s) 368, 1764, 1946
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 7 cut(s) 378, 513, 948, 1098, 1826, 1928, 2133
BfmI CTRYAG 1 cut(s) 1575
BfoI RGCGCY 1 cut(s) 1936
BfuI GTATCC 2 cut(s) 1068, 1646
BglI GCCNNNNNGGC 2 cut(s) 162, 1763
BglII AGATCT 1 cut(s) 1324
BisI GCNGC 3 cut(s) 215, 1026, 1731
BlpI GCTNAGC 1 cut(s) 1233
BlsI GCNGC 3 cut(s) 216, 1027, 1732
Bme1390I CCNGG 5 cut(s) 38, 1185, 1305, 1370, 1735
Bme18I GGWCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
BmgBI CACGTC 1 cut(s) 2077
BmgT120I GGNCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
BmiI GGNNCC 8 cut(s) 6, 189, 573, 1302, 1674, 1949, 2022, 2152
BmrFI CCNGG 5 cut(s) 38, 1185, 1305, 1370, 1735
BmrI ACTGGG 1 cut(s) 2068
BmsI GCATC 6 cut(s) 607, 841, 863, 868, 2130, 2155
BmtI GCTAGC 1 cut(s) 2136
BmuI ACTGGG 1 cut(s) 2068
BpiI GAAGAC 3 cut(s) 2061, 2094, 2250
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 896
Bpu1102I GCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 5 cut(s) 71, 371, 392, 1070, 2114
BpuMI CCSGG 2 cut(s) 38, 1735
BsaAI YACGTR 1 cut(s) 657
BsaJI CCNNGG 5 cut(s) 472, 1304, 1368, 1734, 2169
BsaWI WCCGGW 1 cut(s) 577
BsaXI ACNNNNNCTCC 2 cut(s) 918, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 1439
Bse1I ACTGG 6 cut(s) 364, 751, 1233, 1526, 1639, 2063
Bse3DI GCAATG 3 cut(s) 172, 1174, 1443
BseAI TCCGGA 1 cut(s) 577
BseBI CCWGG 3 cut(s) 1185, 1305, 1370
BseDI CCNNGG 5 cut(s) 472, 1304, 1368, 1734, 2169
BseGI GGATG 5 cut(s) 202, 304, 856, 1284, 2115
BseLI CCNNNNNNNGG 2 cut(s) 299, 1439
BseMI GCAATG 3 cut(s) 172, 1174, 1443
BseMII CTCAG 6 cut(s) 606, 888, 1224, 1864, 1957, 1993
BseNI ACTGG 6 cut(s) 364, 751, 1233, 1526, 1639, 2063
BseRI GAGGAG 2 cut(s) 284, 1520
BseSI GKGCMC 2 cut(s) 574, 1289
BseXI GCAGC 3 cut(s) 226, 1037, 1717
BsgI GTGCAG 1 cut(s) 1044
Bsh1236I CGCG 1 cut(s) 1845
BshFI GGCC 3 cut(s) 156, 1432, 1757
BshNI GGYRCC 1 cut(s) 571
BsiHKAI GWGCWC 2 cut(s) 481, 2084
BsiSI CCGG 5 cut(s) 38, 194, 296, 578, 1734
BslFI GGGAC 3 cut(s) 454, 804, 1961
BslI CCNNNNNNNGG 2 cut(s) 299, 1439
BsmAI GTCTC 3 cut(s) 368, 1764, 1946
BsmBI CGTCTC 1 cut(s) 1946
BsmFI GGGAC 3 cut(s) 454, 804, 1961
BsmI GAATGC 1 cut(s) 1732
BsnI GGCC 3 cut(s) 156, 1432, 1757
Bsp1286I GDGCHC 5 cut(s) 481, 574, 1289, 2084, 2183
Bsp13I TCCGGA 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 1119
Bsp143I GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
Bsp1720I GCTNAGC 1 cut(s) 1233
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 3 cut(s) 191, 293, 1812
BspANI GGCC 3 cut(s) 156, 1432, 1757
BspCNI CTCAG 6 cut(s) 605, 887, 1225, 1863, 1956, 1992
BspEI TCCGGA 1 cut(s) 577
BspFNI CGCG 1 cut(s) 1845
BspHI TCATGA 2 cut(s) 661, 1701
BspLI GGNNCC 8 cut(s) 6, 189, 573, 1302, 1674, 1949, 2022, 2152
BspMAI CTGCAG 1 cut(s) 1579
BspOI GCTAGC 1 cut(s) 2136
BspPI GGATC 4 cut(s) 1095, 1350, 2015, 2028
BspQI GCTCTTC 2 cut(s) 466, 951
BspT107I GGYRCC 1 cut(s) 571
BsrDI GCAATG 3 cut(s) 172, 1174, 1443
BsrGI TGTACA 1 cut(s) 1119
BsrI ACTGG 6 cut(s) 364, 751, 1233, 1526, 1639, 2063
BssECI CCNNGG 5 cut(s) 472, 1304, 1368, 1734, 2169
BssMI GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 1 cut(s) 472
Bst2BI CACGAG 1 cut(s) 536
Bst2UI CCWGG 3 cut(s) 1185, 1305, 1370
Bst4CI ACNGT 5 cut(s) 527, 818, 832, 1247, 1808
Bst6I CTCTTC 6 cut(s) 239, 434, 466, 951, 1421, 1572
BstAUI TGTACA 1 cut(s) 1119
BstBAI YACGTR 1 cut(s) 657
BstC8I GCNNGC 6 cut(s) 154, 1095, 1434, 1831, 2038, 2134
BstDEI CTNAG 8 cut(s) 592, 800, 874, 896, 1233, 1850, 1943, 1979
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 5 cut(s) 202, 304, 856, 1284, 2115
BstFNI CGCG 1 cut(s) 1845
BstH2I RGCGCY 1 cut(s) 1936
BstHHI GCGC 2 cut(s) 349, 1935
BstKTI GATC 7 cut(s) 667, 769, 1103, 1327, 1345, 1537, 2023
BstMAI GTCTC 3 cut(s) 368, 1764, 1946
BstMBI GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
BstMWI GCNNNNNNNGC 5 cut(s) 162, 1475, 1763, 1835, 1932
BstNI CCWGG 3 cut(s) 1185, 1305, 1370
BstNSI RCATGY 1 cut(s) 1082
BstSCI CCNGG 5 cut(s) 36, 1183, 1303, 1368, 1733
BstSFI CTRYAG 1 cut(s) 1575
BstSLI GKGCMC 2 cut(s) 574, 1289
BstUI CGCG 1 cut(s) 1845
BstV1I GCAGC 3 cut(s) 226, 1037, 1717
BstV2I GAAGAC 3 cut(s) 2061, 2094, 2250
BstX2I RGATCY 3 cut(s) 1100, 1324, 2020
BstYI RGATCY 3 cut(s) 1100, 1324, 2020
BsuI GTATCC 2 cut(s) 1068, 1646
BsuRI GGCC 3 cut(s) 156, 1432, 1757
BtgI CCRYGG 1 cut(s) 472
BtrI CACGTC 1 cut(s) 2077
BtsCI GGATG 5 cut(s) 202, 304, 856, 1284, 2115
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 3 cut(s) 49, 776, 1243
Cac8I GCNNGC 6 cut(s) 154, 1095, 1434, 1831, 2038, 2134
CciI TCATGA 2 cut(s) 661, 1701
CfoI GCGC 2 cut(s) 349, 1935
Cfr13I GGNCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
Csp6I GTAC 6 cut(s) 814, 1120, 1203, 1510, 1519, 1966
CviQI GTAC 6 cut(s) 814, 1120, 1203, 1510, 1519, 1966
DdeI CTNAG 8 cut(s) 592, 800, 874, 896, 1233, 1850, 1943, 1979
DpnI GATC 7 cut(s) 666, 768, 1102, 1326, 1344, 1536, 2022
DpnII GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
DraI TTTAAA 1 cut(s) 1140
DraIII CACNNNGTG 1 cut(s) 320
EaeI YGGCCR 1 cut(s) 1755
Eam1104I CTCTTC 6 cut(s) 239, 434, 466, 951, 1421, 1572
EarI CTCTTC 6 cut(s) 239, 434, 466, 951, 1421, 1572
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 472
Eco147I AGGCCT 1 cut(s) 1432
Eco24I GRGCYC 1 cut(s) 2183
Eco32I GATATC 1 cut(s) 1624
Eco47I GGWCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
EcoO109I RGGNCCY 3 cut(s) 1388, 1948, 2150
EcoRI GAATTC 2 cut(s) 78, 688
EcoRII CCWGG 3 cut(s) 1183, 1303, 1368
EcoRV GATATC 1 cut(s) 1624
EcoT14I CCWWGG 1 cut(s) 472
EcoT22I ATGCAT 1 cut(s) 856
EcoT38I GRGCYC 1 cut(s) 2183
ErhI CCWWGG 1 cut(s) 472
Esp3I CGTCTC 1 cut(s) 1946
FalI AAGNNNNNCTT 2 cut(s) 1144, 1176
FaqI GGGAC 3 cut(s) 454, 804, 1961
FauI CCCGC 1 cut(s) 300
FbaI TGATCA 1 cut(s) 766
Fnu4HI GCNGC 3 cut(s) 215, 1026, 1731
FokI GGATG 5 cut(s) 209, 311, 863, 1291, 2122
FriOI GRGCYC 1 cut(s) 2183
Fsp4HI GCNGC 3 cut(s) 215, 1026, 1731
FspBI CTAG 7 cut(s) 378, 513, 948, 1098, 1826, 1928, 2133
GlaI GCGC 2 cut(s) 348, 1934
GluI GCNGC 3 cut(s) 215, 1026, 1731
GsuI CTGGAG 1 cut(s) 381
HaeII RGCGCY 1 cut(s) 1936
HaeIII GGCC 3 cut(s) 156, 1432, 1757
HapII CCGG 5 cut(s) 38, 194, 296, 578, 1734
HhaI GCGC 2 cut(s) 349, 1935
Hin6I GCGC 2 cut(s) 347, 1933
HinP1I GCGC 2 cut(s) 347, 1933
HincII GTYRAC 3 cut(s) 357, 987, 1800
HindII GTYRAC 3 cut(s) 357, 987, 1800
HindIII AAGCTT 1 cut(s) 260
HpaII CCGG 5 cut(s) 38, 194, 296, 578, 1734
HphI GGTGA 5 cut(s) 314, 406, 1273, 2115, 2210
HpyAV CCTTC 6 cut(s) 154, 735, 818, 975, 1146, 1610
HpyCH4III ACNGT 5 cut(s) 527, 818, 832, 1247, 1808
HpyCH4IV ACGT 5 cut(s) 20, 656, 734, 1939, 2076
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 1475, 1763, 1835, 1932
HpyF3I CTNAG 8 cut(s) 592, 800, 874, 896, 1233, 1850, 1943, 1979
HpySE526I ACGT 5 cut(s) 20, 656, 734, 1939, 2076
HspAI GCGC 2 cut(s) 347, 1933
Kpn2I TCCGGA 1 cut(s) 577
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
LguI GCTCTTC 2 cut(s) 466, 951
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 3 cut(s) 226, 1037, 1717
LweI GCATC 6 cut(s) 607, 841, 863, 868, 2130, 2155
MaeI CTAG 7 cut(s) 378, 513, 948, 1098, 1826, 1928, 2133
MaeII ACGT 5 cut(s) 20, 656, 734, 1939, 2076
MaeIII GTNAC 5 cut(s) 21, 1189, 1261, 1334, 2062
MalI GATC 7 cut(s) 666, 768, 1102, 1326, 1344, 1536, 2022
MboI GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
MfeI CAATTG 3 cut(s) 543, 621, 1869
MflI RGATCY 3 cut(s) 1100, 1324, 2020
MhlI GDGCHC 5 cut(s) 481, 574, 1289, 2084, 2183
MlsI TGGCCA 1 cut(s) 1757
MluNI TGGCCA 1 cut(s) 1757
MlyI GAGTC 4 cut(s) 305, 1197, 1971, 2114
MmeI TCCRAC 4 cut(s) 628, 656, 898, 2087
Mox20I TGGCCA 1 cut(s) 1757
Mph1103I ATGCAT 1 cut(s) 856
MroI TCCGGA 1 cut(s) 577
MscI TGGCCA 1 cut(s) 1757
MseI TTAA 7 cut(s) 63, 138, 209, 240, 1139, 1482, 1590
Msp20I TGGCCA 1 cut(s) 1757
MspI CCGG 5 cut(s) 38, 194, 296, 578, 1734
MspR9I CCNGG 5 cut(s) 38, 1185, 1305, 1370, 1735
MunI CAATTG 3 cut(s) 543, 621, 1869
Mva1269I GAATGC 1 cut(s) 1732
MvaI CCWGG 3 cut(s) 1185, 1305, 1370
MvnI CGCG 1 cut(s) 1845
MwoI GCNNNNNNNGC 5 cut(s) 162, 1475, 1763, 1835, 1932
NciI CCSGG 2 cut(s) 38, 1735
NcoI CCATGG 1 cut(s) 472
NdeII GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
NheI GCTAGC 1 cut(s) 2132
NlaIV GGNNCC 8 cut(s) 6, 189, 573, 1302, 1674, 1949, 2022, 2152
NmeAIII GCCGAG 1 cut(s) 2194
NmuCI GTSAC 4 cut(s) 21, 1189, 1261, 1334
NsiI ATGCAT 1 cut(s) 856
NspI RCATGY 1 cut(s) 1082
PagI TCATGA 2 cut(s) 661, 1701
PceI AGGCCT 1 cut(s) 1432
PciSI GCTCTTC 2 cut(s) 466, 951
PctI GAATGC 1 cut(s) 1732
PfeI GAWTC 6 cut(s) 127, 325, 397, 842, 1015, 1891
PflFI GACNNNGTC 1 cut(s) 1952
PkrI GCNGC 3 cut(s) 216, 1027, 1732
PleI GAGTC 4 cut(s) 305, 1196, 1971, 2114
PpsI GAGTC 4 cut(s) 305, 1196, 1971, 2114
Ppu21I YACGTR 1 cut(s) 657
PpuMI RGGWCCY 3 cut(s) 1388, 1948, 2150
PsiI TTATAA 2 cut(s) 866, 1331
Psp5II RGGWCCY 3 cut(s) 1388, 1948, 2150
Psp6I CCWGG 3 cut(s) 1183, 1303, 1368
PspGI CCWGG 3 cut(s) 1183, 1303, 1368
PspN4I GGNNCC 8 cut(s) 6, 189, 573, 1302, 1674, 1949, 2022, 2152
PspPI GGNCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
PspPPI RGGWCCY 3 cut(s) 1388, 1948, 2150
PstI CTGCAG 1 cut(s) 1579
PsuI RGATCY 3 cut(s) 1100, 1324, 2020
PsyI GACNNNGTC 1 cut(s) 1952
RsaI GTAC 6 cut(s) 815, 1121, 1204, 1511, 1520, 1967
RsaNI GTAC 6 cut(s) 814, 1120, 1203, 1510, 1519, 1966
SapI GCTCTTC 2 cut(s) 466, 951
SaqAI TTAA 7 cut(s) 63, 138, 209, 240, 1139, 1482, 1590
SatI GCNGC 3 cut(s) 215, 1026, 1731
Sau3AI GATC 7 cut(s) 664, 766, 1100, 1324, 1342, 1534, 2020
Sau96I GGNCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
SchI GAGTC 4 cut(s) 305, 1197, 1971, 2114
ScrFI CCNGG 5 cut(s) 38, 1185, 1305, 1370, 1735
SduI GDGCHC 5 cut(s) 481, 574, 1289, 2084, 2183
SfaNI GCATC 6 cut(s) 607, 841, 863, 868, 2130, 2155
SfcI CTRYAG 1 cut(s) 1575
SinI GGWCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
SmlI CTYRAG 5 cut(s) 86, 350, 371, 1049, 2093
SmoI CTYRAG 5 cut(s) 86, 350, 371, 1049, 2093
SpeI ACTAGT 1 cut(s) 377
SseBI AGGCCT 1 cut(s) 1432
SsiI CCGC 3 cut(s) 191, 293, 1812
SspI AATATT 3 cut(s) 715, 1528, 1602
SspMI CTAG 7 cut(s) 378, 513, 948, 1098, 1826, 1928, 2133
StuI AGGCCT 1 cut(s) 1432
StyD4I CCNGG 5 cut(s) 36, 1183, 1303, 1368, 1733
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 5 cut(s) 527, 818, 832, 1247, 1808
TaiI ACGT 5 cut(s) 23, 659, 737, 1942, 2079
TaqI TCGA 2 cut(s) 383, 400
TatI WGTACW 5 cut(s) 813, 1119, 1509, 1518, 1965
TfiI GAWTC 6 cut(s) 127, 325, 397, 842, 1015, 1891
Tru1I TTAA 7 cut(s) 63, 138, 209, 240, 1139, 1482, 1590
Tru9I TTAA 7 cut(s) 63, 138, 209, 240, 1139, 1482, 1590
TscAI CASTG 3 cut(s) 56, 776, 1250
TseFI GTSAC 4 cut(s) 21, 1189, 1261, 1334
TseI GCWGC 3 cut(s) 214, 1025, 1730
Tsp45I GTSAC 4 cut(s) 21, 1189, 1261, 1334
TspDTI ATGAA 6 cut(s) 119, 453, 1559, 1707, 1718, 2247
TspRI CASTG 3 cut(s) 56, 776, 1250
Tth111I GACNNNGTC 1 cut(s) 1952
VpaK11BI GGWCC 5 cut(s) 1155, 1356, 1388, 1948, 2150
XapI RAATTY 4 cut(s) 78, 688, 868, 1558
XbaI TCTAGA 1 cut(s) 512
XceI RCATGY 1 cut(s) 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XspI CTAG 7 cut(s) 378, 513, 948, 1098, 1826, 1928, 2133
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.