Protein of unknown function (DUF 659)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 407 |
| Acc36I | ACCTGC | 3 cut(s) | 407, 617, 724 |
| AccB1I | GGYRCC | 1 cut(s) | 779 |
| AccBSI | CCGCTC | 1 cut(s) | 251 |
| AciI | CCGC | 3 cut(s) | 249, 621, 824 |
| AclWI | GGATC | 1 cut(s) | 731 |
| AcsI | RAATTY | 1 cut(s) | 269 |
| AcuI | CTGAAG | 1 cut(s) | 839 |
| AfaI | GTAC | 2 cut(s) | 419, 791 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 129, 554, 602 |
| AgsI | TTSAA | 2 cut(s) | 196, 234 |
| AjnI | CCWGG | 1 cut(s) | 912 |
| AleI | CACNNNNGTG | 1 cut(s) | 785 |
| AluBI | AGCT | 2 cut(s) | 678, 908 |
| AluI | AGCT | 2 cut(s) | 678, 908 |
| AlwI | GGATC | 1 cut(s) | 731 |
| ApeKI | GCWGC | 3 cut(s) | 651, 732, 905 |
| ApoI | RAATTY | 1 cut(s) | 269 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 211 |
| AspLEI | GCGC | 4 cut(s) | 404, 425, 626, 656 |
| AspS9I | GGNCC | 1 cut(s) | 388 |
| AsuHPI | GGTGA | 4 cut(s) | 344, 388, 767, 991 |
| AvaII | GGWCC | 1 cut(s) | 388 |
| BaeGI | GKGCMC | 1 cut(s) | 782 |
| BanI | GGYRCC | 1 cut(s) | 779 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 1021 |
| BbsI | GAAGAC | 2 cut(s) | 59, 206 |
| BbvI | GCAGC | 3 cut(s) | 638, 744, 917 |
| BccI | CCATC | 5 cut(s) | 118, 605, 699, 822, 946 |
| BceAI | ACGGC | 1 cut(s) | 436 |
| BciT130I | CCWGG | 1 cut(s) | 914 |
| BclI | TGATCA | 1 cut(s) | 843 |
| BfaI | CTAG | 1 cut(s) | 878 |
| BfmI | CTRYAG | 1 cut(s) | 903 |
| BfuAI | ACCTGC | 3 cut(s) | 407, 617, 724 |
| BisI | GCNGC | 4 cut(s) | 622, 652, 733, 906 |
| BlsI | GCNGC | 4 cut(s) | 623, 653, 734, 907 |
| Bme1390I | CCNGG | 1 cut(s) | 914 |
| Bme18I | GGWCC | 1 cut(s) | 388 |
| BmgT120I | GGNCC | 1 cut(s) | 388 |
| BmiI | GGNNCC | 2 cut(s) | 486, 781 |
| BmrFI | CCNGG | 1 cut(s) | 914 |
| BmsI | GCATC | 8 cut(s) | 19, 327, 608, 630, 652, 728, 876, 926 |
| BpiI | GAAGAC | 2 cut(s) | 59, 206 |
| BpmI | CTGGAG | 1 cut(s) | 474 |
| BpuEI | CTTGAG | 1 cut(s) | 906 |
| Bsa29I | ATCGAT | 1 cut(s) | 116 |
| BsaJI | CCNNGG | 3 cut(s) | 264, 480, 940 |
| BsaWI | WCCGGW | 1 cut(s) | 385 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 129, 554, 602 |
| Bse118I | RCCGGY | 1 cut(s) | 404 |
| Bse1I | ACTGG | 3 cut(s) | 491, 534, 548 |
| Bse3DI | GCAATG | 3 cut(s) | 514, 793, 1024 |
| BseBI | CCWGG | 1 cut(s) | 914 |
| BseCI | ATCGAT | 1 cut(s) | 116 |
| BseDI | CCNNGG | 3 cut(s) | 264, 480, 940 |
| BseGI | GGATG | 4 cut(s) | 361, 505, 643, 691 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 129, 554, 602 |
| BseMI | GCAATG | 3 cut(s) | 514, 793, 1024 |
| BseNI | ACTGG | 3 cut(s) | 491, 534, 548 |
| BseRI | GAGGAG | 3 cut(s) | 303, 347, 477 |
| BseSI | GKGCMC | 1 cut(s) | 782 |
| BseXI | GCAGC | 3 cut(s) | 638, 744, 917 |
| BseYI | CCCAGC | 1 cut(s) | 887 |
| BsgI | GTGCAG | 1 cut(s) | 781 |
| BshNI | GGYRCC | 1 cut(s) | 779 |
| BshVI | ATCGAT | 1 cut(s) | 116 |
| BsiSI | CCGG | 2 cut(s) | 386, 405 |
| BslFI | GGGAC | 2 cut(s) | 260, 913 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 129, 554, 602 |
| BsmFI | GGGAC | 2 cut(s) | 260, 913 |
| Bsp1286I | GDGCHC | 1 cut(s) | 782 |
| Bsp143I | GATC | 3 cut(s) | 736, 843, 1004 |
| Bsp19I | CCATGG | 1 cut(s) | 264 |
| BspACI | CCGC | 3 cut(s) | 249, 621, 824 |
| BspDI | ATCGAT | 1 cut(s) | 116 |
| BspHI | TCATGA | 1 cut(s) | 466 |
| BspLI | GGNNCC | 2 cut(s) | 486, 781 |
| BspMAI | CTGCAG | 1 cut(s) | 907 |
| BspMI | ACCTGC | 3 cut(s) | 407, 617, 724 |
| BspPI | GGATC | 1 cut(s) | 731 |
| BspT107I | GGYRCC | 1 cut(s) | 779 |
| BsrBI | CCGCTC | 1 cut(s) | 251 |
| BsrDI | GCAATG | 3 cut(s) | 514, 793, 1024 |
| BsrFI | RCCGGY | 1 cut(s) | 404 |
| BsrI | ACTGG | 3 cut(s) | 491, 534, 548 |
| BssAI | RCCGGY | 1 cut(s) | 404 |
| BssECI | CCNNGG | 3 cut(s) | 264, 480, 940 |
| BssMI | GATC | 3 cut(s) | 736, 843, 1004 |
| BssT1I | CCWWGG | 3 cut(s) | 264, 480, 940 |
| Bst2UI | CCWGG | 1 cut(s) | 914 |
| Bst4CI | ACNGT | 1 cut(s) | 802 |
| Bst6I | CTCTTC | 3 cut(s) | 398, 764, 1006 |
| BstC8I | GCNNGC | 5 cut(s) | 406, 427, 593, 680, 910 |
| BstDEI | CTNAG | 1 cut(s) | 704 |
| BstDSI | CCRYGG | 1 cut(s) | 264 |
| BstF5I | GGATG | 4 cut(s) | 361, 505, 643, 691 |
| BstHHI | GCGC | 4 cut(s) | 404, 425, 626, 656 |
| BstKTI | GATC | 3 cut(s) | 739, 846, 1007 |
| BstMBI | GATC | 3 cut(s) | 736, 843, 1004 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 830, 914 |
| BstNI | CCWGG | 1 cut(s) | 914 |
| BstNSI | RCATGY | 1 cut(s) | 895 |
| BstSCI | CCNGG | 1 cut(s) | 912 |
| BstSFI | CTRYAG | 1 cut(s) | 903 |
| BstSLI | GKGCMC | 1 cut(s) | 782 |
| BstV1I | GCAGC | 3 cut(s) | 638, 744, 917 |
| BstV2I | GAAGAC | 2 cut(s) | 59, 206 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 895 |
| Bsu15I | ATCGAT | 1 cut(s) | 116 |
| BsuTUI | ATCGAT | 1 cut(s) | 116 |
| BtgI | CCRYGG | 1 cut(s) | 264 |
| BtsCI | GGATG | 4 cut(s) | 361, 505, 643, 691 |
| BveI | ACCTGC | 3 cut(s) | 407, 617, 724 |
| Cac8I | GCNNGC | 5 cut(s) | 406, 427, 593, 680, 910 |
| CciI | TCATGA | 1 cut(s) | 466 |
| CfoI | GCGC | 4 cut(s) | 404, 425, 626, 656 |
| Cfr10I | RCCGGY | 1 cut(s) | 404 |
| Cfr13I | GGNCC | 1 cut(s) | 388 |
| ClaI | ATCGAT | 1 cut(s) | 116 |
| Csp6I | GTAC | 2 cut(s) | 418, 790 |
| CviAII | CATG | 6 cut(s) | 265, 374, 467, 658, 821, 892 |
| CviJI | RGCY | 9 cut(s) | 408, 485, 533, 595, 606, 678, 886, 908, 961 |
| CviKI_1 | RGCY | 9 cut(s) | 408, 485, 533, 595, 606, 678, 886, 908, 961 |
| CviQI | GTAC | 2 cut(s) | 418, 790 |
| DdeI | CTNAG | 1 cut(s) | 704 |
| DpnI | GATC | 3 cut(s) | 738, 845, 1006 |
| DpnII | GATC | 3 cut(s) | 736, 843, 1004 |
| Eam1104I | CTCTTC | 3 cut(s) | 398, 764, 1006 |
| EarI | CTCTTC | 3 cut(s) | 398, 764, 1006 |
| Eco130I | CCWWGG | 3 cut(s) | 264, 480, 940 |
| Eco47I | GGWCC | 1 cut(s) | 388 |
| Eco57I | CTGAAG | 1 cut(s) | 839 |
| EcoRII | CCWGG | 1 cut(s) | 912 |
| EcoT14I | CCWWGG | 3 cut(s) | 264, 480, 940 |
| EcoT22I | ATGCAT | 1 cut(s) | 645 |
| ErhI | CCWWGG | 3 cut(s) | 264, 480, 940 |
| FaeI | CATG | 6 cut(s) | 268, 377, 470, 661, 824, 895 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 274, 306 |
| FaqI | GGGAC | 2 cut(s) | 260, 913 |
| FatI | CATG | 6 cut(s) | 264, 373, 466, 657, 820, 891 |
| FbaI | TGATCA | 1 cut(s) | 843 |
| Fnu4HI | GCNGC | 4 cut(s) | 622, 652, 733, 906 |
| FokI | GGATG | 4 cut(s) | 348, 492, 630, 678 |
| Fsp4HI | GCNGC | 4 cut(s) | 622, 652, 733, 906 |
| FspBI | CTAG | 1 cut(s) | 878 |
| GlaI | GCGC | 4 cut(s) | 403, 424, 625, 655 |
| GluI | GCNGC | 4 cut(s) | 622, 652, 733, 906 |
| GsaI | CCCAGC | 1 cut(s) | 891 |
| GsuI | CTGGAG | 1 cut(s) | 474 |
| HapII | CCGG | 2 cut(s) | 386, 405 |
| HhaI | GCGC | 4 cut(s) | 404, 425, 626, 656 |
| Hin1II | CATG | 6 cut(s) | 268, 377, 470, 661, 824, 895 |
| Hin6I | GCGC | 4 cut(s) | 402, 423, 624, 654 |
| HinP1I | GCGC | 4 cut(s) | 402, 423, 624, 654 |
| HincII | GTYRAC | 1 cut(s) | 446 |
| HindII | GTYRAC | 1 cut(s) | 446 |
| HinfI | GANTC | 2 cut(s) | 230, 502 |
| HpaII | CCGG | 2 cut(s) | 386, 405 |
| HphI | GGTGA | 4 cut(s) | 344, 388, 767, 991 |
| Hpy166II | GTNNAC | 4 cut(s) | 379, 438, 446, 790 |
| Hpy188I | TCNGA | 4 cut(s) | 229, 617, 670, 741 |
| Hpy188III | TCNNGA | 5 cut(s) | 467, 637, 857, 871, 1008 |
| Hpy8I | GTNNAC | 4 cut(s) | 379, 438, 446, 790 |
| HpyAV | CCTTC | 2 cut(s) | 520, 962 |
| HpyCH4III | ACNGT | 1 cut(s) | 802 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 830, 914 |
| HpyF3I | CTNAG | 1 cut(s) | 704 |
| Hsp92II | CATG | 6 cut(s) | 268, 377, 470, 661, 824, 895 |
| HspAI | GCGC | 4 cut(s) | 402, 423, 624, 654 |
| KroI | GCCGGC | 1 cut(s) | 404 |
| KroNI | GCCGGC | 1 cut(s) | 406 |
| Ksp22I | TGATCA | 1 cut(s) | 843 |
| Kzo9I | GATC | 3 cut(s) | 736, 843, 1004 |
| LmnI | GCTCC | 2 cut(s) | 345, 490 |
| Lsp1109I | GCAGC | 3 cut(s) | 638, 744, 917 |
| LweI | GCATC | 8 cut(s) | 19, 327, 608, 630, 652, 728, 876, 926 |
| MaeI | CTAG | 1 cut(s) | 878 |
| MaeIII | GTNAC | 3 cut(s) | 350, 806, 997 |
| MalI | GATC | 3 cut(s) | 738, 845, 1006 |
| MbiI | CCGCTC | 1 cut(s) | 251 |
| MboI | GATC | 3 cut(s) | 736, 843, 1004 |
| MhlI | GDGCHC | 1 cut(s) | 782 |
| MluCI | AATT | 3 cut(s) | 79, 93, 269 |
| MlyI | GAGTC | 1 cut(s) | 511 |
| Mph1103I | ATGCAT | 1 cut(s) | 645 |
| MroNI | GCCGGC | 1 cut(s) | 404 |
| MroXI | GAANNNNTTC | 1 cut(s) | 211 |
| MseI | TTAA | 1 cut(s) | 96 |
| MslI | CAYNNNNRTG | 4 cut(s) | 161, 164, 785, 819 |
| MspI | CCGG | 2 cut(s) | 386, 405 |
| MspR9I | CCNGG | 1 cut(s) | 914 |
| MvaI | CCWGG | 1 cut(s) | 914 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 830, 914 |
| NaeI | GCCGGC | 1 cut(s) | 406 |
| NcoI | CCATGG | 1 cut(s) | 264 |
| NdeII | GATC | 3 cut(s) | 736, 843, 1004 |
| NgoMIV | GCCGGC | 1 cut(s) | 404 |
| NlaIII | CATG | 6 cut(s) | 268, 377, 470, 661, 824, 895 |
| NlaIV | GGNNCC | 2 cut(s) | 486, 781 |
| NmuCI | GTSAC | 3 cut(s) | 350, 806, 997 |
| NsiI | ATGCAT | 1 cut(s) | 645 |
| NspI | RCATGY | 1 cut(s) | 895 |
| OliI | CACNNNNGTG | 1 cut(s) | 785 |
| PagI | TCATGA | 1 cut(s) | 466 |
| PaqCI | CACCTGC | 1 cut(s) | 407 |
| PdiI | GCCGGC | 1 cut(s) | 406 |
| PdmI | GAANNNNTTC | 1 cut(s) | 211 |
| PfeI | GAWTC | 1 cut(s) | 230 |
| PkrI | GCNGC | 4 cut(s) | 623, 653, 734, 907 |
| PleI | GAGTC | 1 cut(s) | 510 |
| PpsI | GAGTC | 1 cut(s) | 510 |
| Psp6I | CCWGG | 1 cut(s) | 912 |
| PspFI | CCCAGC | 1 cut(s) | 887 |
| PspGI | CCWGG | 1 cut(s) | 912 |
| PspN4I | GGNNCC | 2 cut(s) | 486, 781 |
| PspPI | GGNCC | 1 cut(s) | 388 |
| PstI | CTGCAG | 1 cut(s) | 907 |
| RsaI | GTAC | 2 cut(s) | 419, 791 |
| RsaNI | GTAC | 2 cut(s) | 418, 790 |
| RseI | CAYNNNNRTG | 4 cut(s) | 161, 164, 785, 819 |
| SaqAI | TTAA | 1 cut(s) | 96 |
| SatI | GCNGC | 4 cut(s) | 622, 652, 733, 906 |
| Sau3AI | GATC | 3 cut(s) | 736, 843, 1004 |
| Sau96I | GGNCC | 1 cut(s) | 388 |
| SchI | GAGTC | 1 cut(s) | 511 |
| ScrFI | CCNGG | 1 cut(s) | 914 |
| SduI | GDGCHC | 1 cut(s) | 782 |
| SfaNI | GCATC | 8 cut(s) | 19, 327, 608, 630, 652, 728, 876, 926 |
| SfcI | CTRYAG | 1 cut(s) | 903 |
| SinI | GGWCC | 1 cut(s) | 388 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 161, 164, 785, 819 |
| SmlI | CTYRAG | 1 cut(s) | 921 |
| SmoI | CTYRAG | 1 cut(s) | 921 |
| Sse9I | AATT | 3 cut(s) | 79, 93, 269 |
| SsiI | CCGC | 3 cut(s) | 249, 621, 824 |
| SspI | AATATT | 1 cut(s) | 61 |
| SspMI | CTAG | 1 cut(s) | 878 |
| StyD4I | CCNGG | 1 cut(s) | 912 |
| StyI | CCWWGG | 3 cut(s) | 264, 480, 940 |
| TaaI | ACNGT | 1 cut(s) | 802 |
| TaqI | TCGA | 3 cut(s) | 116, 572, 933 |
| TasI | AATT | 3 cut(s) | 79, 93, 269 |
| TauI | GCSGC | 1 cut(s) | 624 |
| TfiI | GAWTC | 1 cut(s) | 230 |
| Tru1I | TTAA | 1 cut(s) | 96 |
| Tru9I | TTAA | 1 cut(s) | 96 |
| TseFI | GTSAC | 3 cut(s) | 350, 806, 997 |
| TseI | GCWGC | 3 cut(s) | 651, 732, 905 |
| Tsp45I | GTSAC | 3 cut(s) | 350, 806, 997 |
| TspDTI | ATGAA | 2 cut(s) | 455, 809 |
| VpaK11BI | GGWCC | 1 cut(s) | 388 |
| XapI | RAATTY | 1 cut(s) | 269 |
| XceI | RCATGY | 1 cut(s) | 895 |
| XmnI | GAANNNNTTC | 1 cut(s) | 211 |
| XspI | CTAG | 1 cut(s) | 878 |
| Zsp2I | ATGCAT | 1 cut(s) | 645 |