Rw5G036050

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000444
Physical Location & Seq
Forward (+)
63146309 .. 63149347
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036050.1

Sequence Viewer

Length: 2499 bp
ATGACATCTTTGGAGGGTTTTGAGCCCATAGCTATTTCCCCTCAGAAACAAGACCCAGGATGGAAACACTGTCAACTTTACATTAAAACTGGGGACCCAAAAGTTGAGTTAAAGAAGTGTTTATATTGTGGCAAAGTGTTTCAGGGTGGAGGGATTAATAGGTTTAAATCTCACCTTGCTGGTAGAAAAGGAAATGGTCCCATTTGTGATCAGGTTCCTCCTCATGTTCGAGTTTCCATGTTGCAGTGCTTAGATGAAAAAATTGCATCTTTAAGGCACAGAAAGAGTCAAATGGCTGTGAACCAGAGTCGTAGTTTTAGTGAACTAGACAATGTGTTTGCTGAAAATGAAGTTGGGGGGCCCAATTTGATGAATCAAGAGGAAGATGTGGGAATGAGCGTTGATAATGGGATTAGGGTTGGTGATAATCTGACTGATGTGCCTCTTGAGATTGATGATTGTTTTAGGAGTGATGGTCTAGAGGGATTGACTACTGTGCCTCTTGCAGCAGTTGACTCGTATAAGGGTAATGCTCTAGGGAGAAAAGCTGGAGTACCGAATCACAGTTTTTCGGAAAATCGAGAGGTGGGTGTTGATAGACGAGTCGGGTGGAGAGGCGGAACTTCTTTTGTAGCTACTACTGCTGGTTTCGGTTCTGGTTCTGCTAATGCTACTAAATTGAGTTATGTCCCAGCTCCTATTCGTAGAAGATCAGTTAGTCATCCCAATTCATATTTGTTTGAAAATGAAACAGATGTTGGCACCAGTAATACAAATGGGAAGAACAGGAAGAGGGTAAGAGATGAAAATGGATCAATGGTTGCTAATGCTGGCACTATTGGCAATTATGGTGAAGTTGAAAAGGCTCCTCTGGGTGCCGTGAAGAACTCTGTTTATTTTCAGCCAATGATTGATGCAATTGCTTCTGGGGGAATGGAGAGTAAACCACCCTCGTATCATGATCTTCAGGGTTGGATATTAAATAATGTGGTTGAAGAAGTAAAGAATGATATTCATCAGCACATAAATACTTGGGAAAGGAATGGTTGCTCTCTTCTAGTAAACCAATTGAACTCCGAAAAGGGTAGAATATTGCTAAATTTCTCAGTGTACAGTCCCGAAGGAACAACATATTTGAAATCTGTGGATGCTTCCTCATTTATAAATTCTCCAGATGCTTTGTATGAGATTCTTAAGCAAGTGTTGGAAGAAGTTGGAGTTAGGCGTGTTTTGCAAGTAATTACAAATAGTAAAGAGCACTATGTTGTTGCTGGGAAGAGGTTGATGGATACGTTTCCTACGCTGTACTGGTCTCCCTGTGCTGCTGCTTGCATAAATCTGATACTTGAGGATTTTGGAAAAGTTGAATTTATCTATAAACACGTCGTCATTCTGAATATGATGCGGAGGTATACTTTTGGAAATGACATTGTGAAGGTGGGAGTAACTCGGTTTGCTACAGACTTTATGACTTTGAAACAAATGGCTGACCTTAAATTCAATTTGCAGACTATGGTTACTTCAAAAGAATGGGAAGGGTGCCCTTATTCTCAAACATCAGAAGGATCAGCAATGCTGGATGTATTAAGTAATCACACATTTTGGTCTTCGTGCATTATGATCACCCAATTAACAAATCCGCTCCTGCGAGTTTTGAGAATTGTTGGTAGCCAGAAGAAGGCAGCAATGGGATATGTATTTGGAGGAATGTATCGAGCAAAAGAAACAATCAAGAGAGAACTTGTCAAGAAAGAAGAATATATGGCTTACTGGAATATTATAGATTACAGGTGGCAAAAATTATGGGATCTTCCTCTTCATGCTGCTGGTTTTTACCTTAATCCCAAGTTTTTCTATAGCATCAAGGGAAAAATGCACAAGGTGATTATGTCAAAAATGCTTGACTGCATAGTGAAATTGGTTCCAGATTTAAAAGTTCAAGACGAAATTAGCAAAGAGATAAACTGGTACAAAAATGCTGTTGGAGATCTGGGGAGGAATTTGGCCATTAGAGCTAGAGACACTCTGCTTCCAGCTGAATGGTGGTCTACATATGGCGGTGGTTGCCCAAATATGGCACGTTTTGCAGTTCATATTCTCAGTCAAACTTGCAGCTTAATTCAGTGCAAGGAGAATCAGATTCCTTTTGACCAACTGCACAAGACAAGGAACAGCTTAGAGCATCAACGGCTTAGTGATTTTGTCTTTGTTCAGTACAACTTGCAACTAAGACAGATGGTTCATAAGAACAAAGAGCATGAGTATGTGGATCCTATATCATTTGACAACACTAGCACTGTTGAAGACTGGATAACAGAGACTGAAATGTACCTAGAAGACCATGAAAATACTGATTGGAAGGCACTTGAACCGCCCTCTTATAATTCAATGCTTTTAGAACTTTCAGTTGATGAAGCTGAAGACTTGGGTTCAGGGTTTGATGATAACGAGATTTTCTTAAACGGATTGCAATTGGTTAAAGAAGAAGGCAGAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

832

Amino Acids

93.86

Weight (kDa)

6.25

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 317 - 460 3.5e-50 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 674 - 741 6.1e-07 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1163, 2382
AccB1I GGYRCC 3 cut(s) 761, 875, 1539
AccBSI CCGCTC 1 cut(s) 1642
AccI GTMKAC 2 cut(s) 1412, 2048
AciI CCGC 5 cut(s) 618, 1405, 1640, 2058, 2372
AclWI GGATC 5 cut(s) 820, 1573, 1815, 2264, 2277
AcoI YGGCCR 1 cut(s) 2004
AcsI RAATTY 5 cut(s) 1099, 1165, 1367, 1496, 1999
AcuI CTGAAG 2 cut(s) 950, 2439
AdeI CACNNNGTG 1 cut(s) 1882
AfaI GTAC 6 cut(s) 555, 1112, 1307, 1970, 2216, 2330
AfiI CCNNNNNNNGG 2 cut(s) 1678, 2074
AflII CTTAAG 1 cut(s) 1193
AflIII ACRYGT 1 cut(s) 1381
AjiI CACGTC 1 cut(s) 1384
AjnI CCWGG 1 cut(s) 55
AjuI GAANNNNNNNTTGG 4 cut(s) 336, 368, 741, 773
AluBI AGCT 9 cut(s) 32, 548, 635, 695, 2015, 2036, 2115, 2175, 2417
AluI AGCT 9 cut(s) 32, 548, 635, 695, 2015, 2036, 2115, 2175, 2417
Alw21I GWGCWC 1 cut(s) 1260
Alw26I GTCTC 3 cut(s) 1317, 2013, 2312
AlwI GGATC 5 cut(s) 820, 1573, 1815, 2264, 2277
AlwNI CAGNNNCTG 1 cut(s) 2321
AoxI GGCC 2 cut(s) 359, 2004
ApaI GGGCCC 1 cut(s) 363
ApeKI GCWGC 6 cut(s) 506, 1322, 1325, 1682, 1823, 2112
ApoI RAATTY 5 cut(s) 1099, 1165, 1367, 1496, 1999
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
AseI ATTAAT 1 cut(s) 156
AspS9I GGNCC 4 cut(s) 94, 197, 359, 360
AsuHPI GGTGA 5 cut(s) 164, 434, 863, 1615, 1894
AvaII GGWCC 2 cut(s) 94, 197
BaeGI GKGCMC 2 cut(s) 363, 1544
BaeI ACNNNNGTAYC 2 cut(s) 938, 971
BalI TGGCCA 1 cut(s) 2006
BamHI GGATCC 1 cut(s) 2269
BanI GGYRCC 3 cut(s) 761, 875, 1539
BanII GRGCYC 2 cut(s) 27, 363
BbsI GAAGAC 4 cut(s) 1599, 2310, 2343, 2427
Bbv12I GWGCWC 1 cut(s) 1260
BbvI GCAGC 6 cut(s) 518, 1309, 1312, 1694, 1810, 2124
BccI CCATC 4 cut(s) 54, 467, 1279, 2230
BceAI ACGGC 2 cut(s) 863, 2204
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BciT130I CCWGG 1 cut(s) 57
BciVI GTATCC 1 cut(s) 1282
BclI TGATCA 2 cut(s) 208, 1620
BcoDI GTCTC 3 cut(s) 1317, 2013, 2312
BfaI CTAG 7 cut(s) 326, 479, 536, 1058, 2016, 2292, 2333
BfmI CTRYAG 2 cut(s) 1458, 1855
BfrI CTTAAG 1 cut(s) 1193
BfuI GTATCC 1 cut(s) 1282
BglII AGATCT 1 cut(s) 1987
BisI GCNGC 6 cut(s) 507, 1323, 1326, 1683, 1824, 2113
BlsI GCNGC 6 cut(s) 508, 1324, 1327, 1684, 1825, 2114
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 2 cut(s) 94, 197
BmgBI CACGTC 1 cut(s) 1384
BmgT120I GGNCC 4 cut(s) 94, 197, 359, 360
BmrFI CCNGG 1 cut(s) 57
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 7 cut(s) 275, 904, 1138, 1165, 1392, 1869, 2191
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 4 cut(s) 1599, 2310, 2343, 2427
BpmI CTGGAG 2 cut(s) 570, 1155
BpuEI CTTGAG 2 cut(s) 467, 1367
BsaBI GATNNNNATC 1 cut(s) 1014
BsaI GGTCTC 1 cut(s) 1317
BsaJI CCNNGG 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 2 cut(s) 1678, 2074
Bse1I ACTGG 6 cut(s) 94, 765, 1313, 1775, 1970, 2312
Bse3DI GCAATG 2 cut(s) 1578, 1692
Bse8I GATNNNNATC 1 cut(s) 1014
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 4 cut(s) 65, 721, 1153, 1585
BseJI GATNNNNATC 1 cut(s) 1014
BseLI CCNNNNNNNGG 2 cut(s) 1678, 2074
BseMI GCAATG 2 cut(s) 1578, 1692
BseMII CTCAG 3 cut(s) 56, 1119, 2113
BseNI ACTGG 6 cut(s) 94, 765, 1313, 1775, 1970, 2312
BseRI GAGGAG 2 cut(s) 210, 858
BseSI GKGCMC 2 cut(s) 363, 1544
BseXI GCAGC 6 cut(s) 518, 1309, 1312, 1694, 1810, 2124
BseYI CCCAGC 2 cut(s) 691, 1271
BsgI GTGCAG 1 cut(s) 2141
BshFI GGCC 2 cut(s) 361, 2006
BshNI GGYRCC 3 cut(s) 761, 875, 1539
BsiHKAI GWGCWC 1 cut(s) 1260
BslFI GGGAC 4 cut(s) 107, 183, 674, 1101
BslI CCNNNNNNNGG 2 cut(s) 1678, 2074
BsmAI GTCTC 3 cut(s) 1317, 2013, 2312
BsmFI GGGAC 4 cut(s) 107, 183, 674, 1101
BsnI GGCC 2 cut(s) 361, 2006
Bso31I GGTCTC 1 cut(s) 1317
Bsp120I GGGCCC 1 cut(s) 359
Bsp1286I GDGCHC 4 cut(s) 27, 363, 1260, 1544
Bsp1407I TGTACA 1 cut(s) 1110
Bsp143I GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
BspACI CCGC 5 cut(s) 618, 1405, 1640, 2058, 2372
BspANI GGCC 2 cut(s) 361, 2006
BspCNI CTCAG 3 cut(s) 55, 1118, 2112
BspHI TCATGA 1 cut(s) 958
BspPI GGATC 5 cut(s) 820, 1573, 1815, 2264, 2277
BspT107I GGYRCC 3 cut(s) 761, 875, 1539
BspTI CTTAAG 1 cut(s) 1193
BspTNI GGTCTC 1 cut(s) 1317
BsrBI CCGCTC 1 cut(s) 1642
BsrDI GCAATG 2 cut(s) 1578, 1692
BsrGI TGTACA 1 cut(s) 1110
BsrI ACTGG 6 cut(s) 94, 765, 1313, 1775, 1970, 2312
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
BssNAI GTATAC 1 cut(s) 1413
Bst1107I GTATAC 1 cut(s) 1413
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 5 cut(s) 71, 496, 566, 1115, 2299
Bst6I CTCTTC 4 cut(s) 785, 1059, 1271, 1821
BstAFI CTTAAG 1 cut(s) 1193
BstAPI GCANNNNNTGC 1 cut(s) 2084
BstAUI TGTACA 1 cut(s) 1110
BstC8I GCNNGC 2 cut(s) 832, 1330
BstDEI CTNAG 7 cut(s) 42, 250, 1105, 2099, 2176, 2192, 2228
BstF5I GGATG 4 cut(s) 65, 721, 1153, 1585
BstKTI GATC 9 cut(s) 211, 713, 815, 964, 1568, 1623, 1810, 1990, 2272
BstMAI GTCTC 3 cut(s) 1317, 2013, 2312
BstMBI GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
BstMWI GCNNNNNNNGC 7 cut(s) 641, 840, 1231, 2012, 2064, 2084, 2188
BstNI CCWGG 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 2 cut(s) 1458, 1855
BstSLI GKGCMC 2 cut(s) 363, 1544
BstV1I GCAGC 6 cut(s) 518, 1309, 1312, 1694, 1810, 2124
BstV2I GAAGAC 4 cut(s) 1599, 2310, 2343, 2427
BstX2I RGATCY 3 cut(s) 1807, 1987, 2269
BstXI CCANNNNNNTGG 1 cut(s) 2040
BstYI RGATCY 3 cut(s) 1807, 1987, 2269
BstZ17I GTATAC 1 cut(s) 1413
BsuI GTATCC 1 cut(s) 1282
BsuRI GGCC 2 cut(s) 361, 2006
BtrI CACGTC 1 cut(s) 1384
BtsCI GGATG 4 cut(s) 65, 721, 1153, 1585
BtsI GCAGTG 1 cut(s) 251
BtsIMutI CAGTG 5 cut(s) 67, 251, 1113, 2129, 2295
Cac8I GCNNGC 2 cut(s) 832, 1330
CaiI CAGNNNCTG 1 cut(s) 2321
CciI TCATGA 1 cut(s) 958
Cfr13I GGNCC 4 cut(s) 94, 197, 359, 360
Csp6I GTAC 6 cut(s) 554, 1111, 1306, 1969, 2215, 2329
CviAII CATG 6 cut(s) 224, 238, 959, 1820, 2258, 2342
CviQI GTAC 6 cut(s) 554, 1111, 1306, 1969, 2215, 2329
DdeI CTNAG 7 cut(s) 42, 250, 1105, 2099, 2176, 2192, 2228
DpnI GATC 9 cut(s) 210, 712, 814, 963, 1567, 1622, 1809, 1989, 2271
DpnII GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
DraI TTTAAA 2 cut(s) 166, 1932
DraIII CACNNNGTG 1 cut(s) 1882
EaeI YGGCCR 1 cut(s) 2004
Eam1104I CTCTTC 4 cut(s) 785, 1059, 1271, 1821
EarI CTCTTC 4 cut(s) 785, 1059, 1271, 1821
EciI GGCGGA 1 cut(s) 633
Eco24I GRGCYC 2 cut(s) 27, 363
Eco31I GGTCTC 1 cut(s) 1317
Eco47I GGWCC 2 cut(s) 94, 197
Eco57I CTGAAG 2 cut(s) 950, 2439
EcoO109I RGGNCCY 2 cut(s) 94, 359
EcoRII CCWGG 1 cut(s) 55
EcoT38I GRGCYC 2 cut(s) 27, 363
FaeI CATG 6 cut(s) 227, 241, 962, 1823, 2261, 2345
FaqI GGGAC 4 cut(s) 107, 183, 674, 1101
FatI CATG 6 cut(s) 223, 237, 958, 1819, 2257, 2341
FauNDI CATATG 1 cut(s) 2053
FbaI TGATCA 2 cut(s) 208, 1620
FblI GTMKAC 2 cut(s) 1412, 2048
Fnu4HI GCNGC 6 cut(s) 507, 1323, 1326, 1683, 1824, 2113
FokI GGATG 4 cut(s) 72, 708, 1160, 1592
FriOI GRGCYC 2 cut(s) 27, 363
Fsp4HI GCNGC 6 cut(s) 507, 1323, 1326, 1683, 1824, 2113
FspBI CTAG 7 cut(s) 326, 479, 536, 1058, 2016, 2292, 2333
GluI GCNGC 6 cut(s) 507, 1323, 1326, 1683, 1824, 2113
GsaI CCCAGC 2 cut(s) 695, 1275
GsuI CTGGAG 2 cut(s) 570, 1155
HaeIII GGCC 2 cut(s) 361, 2006
Hin1II CATG 6 cut(s) 227, 241, 962, 1823, 2261, 2345
HincII GTYRAC 2 cut(s) 74, 514
HindII GTYRAC 2 cut(s) 74, 514
HinfI GANTC 9 cut(s) 286, 307, 373, 515, 559, 603, 1189, 2134, 2140
HphI GGTGA 5 cut(s) 164, 434, 863, 1615, 1894
Hpy166II GTNNAC 9 cut(s) 74, 301, 323, 514, 944, 1063, 1111, 1413, 2049
Hpy188I TCNGA 8 cut(s) 45, 432, 574, 1078, 1341, 1395, 1561, 2139
Hpy8I GTNNAC 9 cut(s) 74, 301, 323, 514, 944, 1063, 1111, 1413, 2049
Hpy99I CGWCG 1 cut(s) 1388
HpyAV CCTTC 7 cut(s) 1115, 1429, 1529, 1556, 1672, 2353, 2480
HpyCH4III ACNGT 5 cut(s) 71, 496, 566, 1115, 2299
HpyCH4IV ACGT 3 cut(s) 1292, 1383, 2080
HpyF10VI GCNNNNNNNGC 7 cut(s) 641, 840, 1231, 2012, 2064, 2084, 2188
HpyF3I CTNAG 7 cut(s) 42, 250, 1105, 2099, 2176, 2192, 2228
HpySE526I ACGT 3 cut(s) 1292, 1383, 2080
Hsp92II CATG 6 cut(s) 227, 241, 962, 1823, 2261, 2345
KflI GGGWCCC 1 cut(s) 94
Ksp22I TGATCA 2 cut(s) 208, 1620
Kzo9I GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
LmnI GCTCC 3 cut(s) 700, 871, 1647
Lsp1109I GCAGC 6 cut(s) 518, 1309, 1312, 1694, 1810, 2124
LweI GCATC 7 cut(s) 275, 904, 1138, 1165, 1392, 1869, 2191
MaeI CTAG 7 cut(s) 326, 479, 536, 1058, 2016, 2292, 2333
MaeII ACGT 3 cut(s) 1292, 1383, 2080
MaeIII GTNAC 2 cut(s) 1444, 1516
MalI GATC 9 cut(s) 210, 712, 814, 963, 1567, 1622, 1809, 1989, 2271
MbiI CCGCTC 1 cut(s) 1642
MboI GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
MfeI CAATTG 3 cut(s) 918, 1067, 2471
MflI RGATCY 3 cut(s) 1807, 1987, 2269
MhlI GDGCHC 4 cut(s) 27, 363, 1260, 1544
MlsI TGGCCA 1 cut(s) 2006
MluNI TGGCCA 1 cut(s) 2006
MlyI GAGTC 4 cut(s) 295, 316, 509, 612
MmeI TCCRAC 4 cut(s) 953, 1185, 1195, 1963
Mox20I TGGCCA 1 cut(s) 2006
MscI TGGCCA 1 cut(s) 2006
MslI CAYNNNNRTG 1 cut(s) 2262
Msp20I TGGCCA 1 cut(s) 2006
MspA1I CMGCKG 1 cut(s) 2036
MspCI CTTAAG 1 cut(s) 1193
MspR9I CCNGG 1 cut(s) 57
MunI CAATTG 3 cut(s) 918, 1067, 2471
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 7 cut(s) 641, 840, 1231, 2012, 2064, 2084, 2188
NdeI CATATG 1 cut(s) 2053
NdeII GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
NlaIII CATG 6 cut(s) 227, 241, 962, 1823, 2261, 2345
PagI TCATGA 1 cut(s) 958
PfeI GAWTC 5 cut(s) 373, 559, 1189, 2134, 2140
PkrI GCNGC 6 cut(s) 508, 1324, 1327, 1684, 1825, 2114
PleI GAGTC 4 cut(s) 294, 315, 509, 611
PpsI GAGTC 4 cut(s) 294, 315, 509, 611
PpuMI RGGWCCY 1 cut(s) 94
PshBI ATTAAT 1 cut(s) 156
PsiI TTATAA 2 cut(s) 1163, 2382
Psp5II RGGWCCY 1 cut(s) 94
Psp6I CCWGG 1 cut(s) 55
PspFI CCCAGC 2 cut(s) 691, 1271
PspGI CCWGG 1 cut(s) 55
PspOMI GGGCCC 1 cut(s) 359
PspPI GGNCC 4 cut(s) 94, 197, 359, 360
PspPPI RGGWCCY 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 2321
PsuI RGATCY 3 cut(s) 1807, 1987, 2269
PvuII CAGCTG 1 cut(s) 2036
RsaI GTAC 6 cut(s) 555, 1112, 1307, 1970, 2216, 2330
RsaNI GTAC 6 cut(s) 554, 1111, 1306, 1969, 2215, 2329
RseI CAYNNNNRTG 1 cut(s) 2262
SatI GCNGC 6 cut(s) 507, 1323, 1326, 1683, 1824, 2113
Sau3AI GATC 9 cut(s) 208, 710, 812, 961, 1565, 1620, 1807, 1987, 2269
Sau96I GGNCC 4 cut(s) 94, 197, 359, 360
SchI GAGTC 4 cut(s) 295, 316, 509, 612
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 4 cut(s) 27, 363, 1260, 1544
SfaNI GCATC 7 cut(s) 275, 904, 1138, 1165, 1392, 1869, 2191
SfcI CTRYAG 2 cut(s) 1458, 1855
SinI GGWCC 2 cut(s) 94, 197
SmiMI CAYNNNNRTG 1 cut(s) 2262
SmlI CTYRAG 3 cut(s) 446, 1193, 1346
SmoI CTYRAG 3 cut(s) 446, 1193, 1346
SsiI CCGC 5 cut(s) 618, 1405, 1640, 2058, 2372
SspI AATATT 2 cut(s) 1092, 1777
SspMI CTAG 7 cut(s) 326, 479, 536, 1058, 2016, 2292, 2333
StyD4I CCNGG 1 cut(s) 55
TaaI ACNGT 5 cut(s) 71, 496, 566, 1115, 2299
TaiI ACGT 3 cut(s) 1295, 1386, 2083
TaqI TCGA 3 cut(s) 229, 580, 1714
TatI WGTACW 3 cut(s) 1110, 1305, 2214
TfiI GAWTC 5 cut(s) 373, 559, 1189, 2134, 2140
TscAI CASTG 5 cut(s) 74, 251, 1113, 2129, 2302
TseI GCWGC 6 cut(s) 506, 1322, 1325, 1682, 1823, 2112
TspGWI ACGGA 1 cut(s) 2478
TspRI CASTG 5 cut(s) 74, 251, 1113, 2129, 2302
Vha464I CTTAAG 1 cut(s) 1193
VpaK11BI GGWCC 2 cut(s) 94, 197
VspI ATTAAT 1 cut(s) 156
XapI RAATTY 5 cut(s) 1099, 1165, 1367, 1496, 1999
XbaI TCTAGA 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 2040
XmiI GTMKAC 2 cut(s) 1412, 2048
XspI CTAG 7 cut(s) 326, 479, 536, 1058, 2016, 2292, 2333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.