pycom01g15810

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000444
Physical Location & Seq
Forward (+)
15565490 .. 15566718
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15810.2

Sequence Viewer

Length: 642 bp
ATGCATAGTTATATTGTCTCGGGTACATTTGATTGCATCGAGAGATTGGTTCCTGATACAAATGTCCAAGATAAAATCATCAAAGAGCTAAATTTGTACAAGAGTGCTGCTGGAGATTTTCGGAGGAAGATGGCAATTAGGGCTAGAGACACTCTGCTTCCTGCTGAATGGTGGTCAACATATGGAGGAGGTTGCCCAAATTTGACACGCTTGGCAATCCGAATTCTCAGTCAAACTTGCAGTTCGATTGGCTGCAGGCGAAATGAAATTCCTTTTGGGAAAGCACACAACACAAGGAACTGCCTTGAGCGTCAACGTGTCAGTGACCTCATCTTTGTTCATTATAACTTGCGACTGAAACAAATGGTTGGTAAGAACATCGGACAAGATGTTATGGATCCCATATCATTTGAAAACATTAGCATGACCGAAGACTGGGTTACCAGGAAGGACATGTGCTTAGATGACAATGGGAGTTCCGACTGGATGGAACTCGATTCAAACTCTGTAAGCACAATGCTTCTAGGGCCGTCAAATGATGATGCCGATGACTTGGGCTCTGGGTTTGACGATTATGAGATCTTTAGTAGAGCGAAAGACGGTGAAGAGGAAAATGTTGAAGACAACGTAGAAAATCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

214

Amino Acids

24.23

Weight (kDa)

4.77

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AclWI GGATC 2 cut(s) 392, 405
AcsI RAATTY 4 cut(s) 91, 199, 222, 267
AfaI GTAC 2 cut(s) 25, 98
AfiI CCNNNNNNNGG 1 cut(s) 435
AflIII ACRYGT 2 cut(s) 316, 453
AgsI TTSAA 3 cut(s) 413, 501, 620
AjnI CCWGG 1 cut(s) 443
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 1 cut(s) 88
AluI AGCT 1 cut(s) 88
Alw26I GTCTC 2 cut(s) 22, 141
AlwI GGATC 2 cut(s) 392, 405
Ama87I CYCGRG 1 cut(s) 19
AoxI GGCC 1 cut(s) 527
ApeKI GCWGC 2 cut(s) 107, 252
ApoI RAATTY 4 cut(s) 91, 199, 222, 267
ArsI GACNNNNNNTTYG 4 cut(s) 214, 246, 317, 349
AspS9I GGNCC 1 cut(s) 527
AsuHPI GGTGA 1 cut(s) 614
AvaI CYCGRG 1 cut(s) 19
BaeI ACNNNNGTAYC 2 cut(s) 48, 81
BamHI GGATCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 560
BbsI GAAGAC 2 cut(s) 438, 627
BbvI GCAGC 2 cut(s) 94, 239
BccI CCATC 2 cut(s) 124, 481
BceAI ACGGC 1 cut(s) 514
BciT130I CCWGG 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 22, 141
BfaI CTAG 2 cut(s) 144, 524
BfmI CTRYAG 1 cut(s) 253
BglII AGATCT 1 cut(s) 579
BisI GCNGC 2 cut(s) 108, 253
BlsI GCNGC 2 cut(s) 109, 254
Bme1390I CCNGG 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 2 cut(s) 51, 399
BmrFI CCNGG 1 cut(s) 445
BmrI ACTGGG 1 cut(s) 445
BmsI GCATC 2 cut(s) 45, 532
BmuI ACTGGG 1 cut(s) 445
BpiI GAAGAC 2 cut(s) 438, 627
BpmI CTGGAG 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 326
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 2 cut(s) 440, 488
BseBI CCWGG 1 cut(s) 445
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 435
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 2 cut(s) 440, 488
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 2 cut(s) 94, 239
BshFI GGCC 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 435
BsmAI GTCTC 2 cut(s) 22, 141
BsnI GGCC 1 cut(s) 529
BsoBI CYCGRG 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 560
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 2 cut(s) 397, 579
BspANI GGCC 1 cut(s) 529
BspCNI CTCAG 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 51, 399
BspMAI CTGCAG 1 cut(s) 257
BspPI GGATC 2 cut(s) 392, 405
BsrGI TGTACA 1 cut(s) 96
BsrI ACTGG 2 cut(s) 440, 488
BssMI GATC 2 cut(s) 397, 579
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 1 cut(s) 602
Bst6I CTCTTC 1 cut(s) 600
BstAUI TGTACA 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 2 cut(s) 227, 460
BstEII GGTNACC 1 cut(s) 439
BstF5I GGATG 1 cut(s) 492
BstKTI GATC 2 cut(s) 400, 582
BstMAI GTCTC 2 cut(s) 22, 141
BstMBI GATC 2 cut(s) 397, 579
BstMWI GCNNNNNNNGC 2 cut(s) 140, 526
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 1 cut(s) 457
BstPI GGTNACC 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 253
BstV1I GCAGC 2 cut(s) 94, 239
BstV2I GAAGAC 2 cut(s) 438, 627
BstX2I RGATCY 2 cut(s) 397, 579
BstYI RGATCY 2 cut(s) 397, 579
BsuRI GGCC 1 cut(s) 529
BtsCI GGATG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 527
CseI GACGC 1 cut(s) 299
Csp6I GTAC 2 cut(s) 24, 97
CviAII CATG 2 cut(s) 424, 454
CviJI RGCY 5 cut(s) 88, 143, 252, 529, 558
CviKI_1 RGCY 5 cut(s) 88, 143, 252, 529, 558
CviQI GTAC 2 cut(s) 24, 97
DdeI CTNAG 2 cut(s) 227, 460
DpnI GATC 2 cut(s) 399, 581
DpnII GATC 2 cut(s) 397, 579
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
Eco24I GRGCYC 1 cut(s) 560
Eco88I CYCGRG 1 cut(s) 19
Eco91I GGTNACC 1 cut(s) 439
EcoO65I GGTNACC 1 cut(s) 439
EcoRI GAATTC 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 443
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 560
FaeI CATG 2 cut(s) 427, 457
FatI CATG 2 cut(s) 423, 453
FauNDI CATATG 1 cut(s) 181
Fnu4HI GCNGC 2 cut(s) 108, 253
FokI GGATG 1 cut(s) 499
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 2 cut(s) 108, 253
FspBI CTAG 2 cut(s) 144, 524
GluI GCNGC 2 cut(s) 108, 253
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 1 cut(s) 529
HgaI GACGC 1 cut(s) 299
Hin1II CATG 2 cut(s) 427, 457
HincII GTYRAC 2 cut(s) 177, 314
HindII GTYRAC 2 cut(s) 177, 314
HinfI GANTC 1 cut(s) 497
HphI GGTGA 1 cut(s) 614
Hpy166II GTNNAC 2 cut(s) 177, 314
Hpy188I TCNGA 4 cut(s) 123, 221, 383, 481
Hpy188III TCNNGA 2 cut(s) 40, 53
Hpy8I GTNNAC 2 cut(s) 177, 314
HpyAV CCTTC 1 cut(s) 442
HpyCH4III ACNGT 1 cut(s) 602
HpyCH4IV ACGT 2 cut(s) 316, 627
HpyCH4V TGCA 4 cut(s) 4, 36, 240, 255
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 526
HpyF3I CTNAG 2 cut(s) 227, 460
HpySE526I ACGT 2 cut(s) 316, 627
Hsp92II CATG 2 cut(s) 427, 457
Kzo9I GATC 2 cut(s) 397, 579
LpnPI CCDG 9 cut(s) 66, 96, 174, 241, 421, 430, 457, 469, 546
Lsp1109I GCAGC 2 cut(s) 94, 239
LweI GCATC 2 cut(s) 45, 532
MaeI CTAG 2 cut(s) 144, 524
MaeII ACGT 2 cut(s) 316, 627
MaeIII GTNAC 2 cut(s) 323, 439
MalI GATC 2 cut(s) 399, 581
MboI GATC 2 cut(s) 397, 579
MboII GAAGA 4 cut(s) 139, 443, 617, 632
MflI RGATCY 2 cut(s) 397, 579
MhlI GDGCHC 1 cut(s) 560
MluCI AATT 5 cut(s) 91, 135, 199, 222, 267
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 5 cut(s) 117, 179, 182, 338, 601
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 2 cut(s) 140, 526
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 397, 579
NlaIII CATG 2 cut(s) 427, 457
NlaIV GGNNCC 2 cut(s) 51, 399
NmuCI GTSAC 1 cut(s) 323
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 457
PciI ACATGT 1 cut(s) 453
PfeI GAWTC 1 cut(s) 497
PkrI GCNGC 2 cut(s) 109, 254
PscI ACATGT 1 cut(s) 453
PsiI TTATAA 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 443
PspEI GGTNACC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 2 cut(s) 51, 399
PspPI GGNCC 1 cut(s) 527
PstI CTGCAG 1 cut(s) 257
PsuI RGATCY 2 cut(s) 397, 579
RsaI GTAC 2 cut(s) 25, 98
RsaNI GTAC 2 cut(s) 24, 97
RseI CAYNNNNRTG 1 cut(s) 422
SatI GCNGC 2 cut(s) 108, 253
Sau3AI GATC 2 cut(s) 397, 579
Sau96I GGNCC 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 1 cut(s) 560
SetI ASST 5 cut(s) 90, 193, 319, 330, 630
SfaNI GCATC 2 cut(s) 45, 532
SfcI CTRYAG 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 5 cut(s) 91, 135, 199, 222, 267
SspMI CTAG 2 cut(s) 144, 524
StyD4I CCNGG 1 cut(s) 443
TaaI ACNGT 1 cut(s) 602
TaiI ACGT 2 cut(s) 319, 630
TaqI TCGA 3 cut(s) 39, 245, 495
TaqII GACCGA 1 cut(s) 443
TasI AATT 5 cut(s) 91, 135, 199, 222, 267
TatI WGTACW 1 cut(s) 96
TfiI GAWTC 1 cut(s) 497
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 2 cut(s) 107, 252
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 2 cut(s) 279, 329
TspRI CASTG 1 cut(s) 328
XapI RAATTY 4 cut(s) 91, 199, 222, 267
XceI RCATGY 1 cut(s) 457
XspI CTAG 2 cut(s) 144, 524
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.