pycom02g22730

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000444
Physical Location & Seq
Forward (+)
20920163 .. 20924358
4196 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22730.4

Sequence Viewer

Length: 2304 bp
ATGCAGCACAAGGATAATCAAGGTTCAGTTCCACCTCGTGCTTCTGATCCTGGCTGGGCCCATGGCATTATGGTGAATGGAGGTCGTCAGAAGATTAAGTGCAAATACTGCCACAAAGTAATGCTTGGGGGTGGAATTTCCAGACTAAAACAGCATCTGGCAGGGGAAAGGGGGAATGTAGCTCCATGCGAGGAAGTACCTGAAGAAGTTAAAATGCAGATTCAACACCACCTAGGTTTTAAAGTACTTGAGAAGCTTAAAAAGCAGAAAGGATTAAGCACTAGCAGGGATTCCGCATCCTACCAGTATAGGGAAGAAGACAATGAAGATGGAATGAGAACTCAAAAGAAAGTTTCCACACGAGGCACGCGAAGGAGAAAGGGAAAAGAGGCTACAGAAGGAATTTCCAATAAGAAACACAAAAAGCATTCTTTTCCTGCTGCAACAGCTAATATCGCTCAGCCCTTGCATCAGAGTTTTGCTTCTCAAGATATGATAGATCAAGCTGATTTGGCTGTTGCAAGATTTATGTATGAATCTGGTATACCATTCGCTGCTGCAAATTCTCATTTTTATCAACAGATGGCTGATGCCATTGCTGCTGTCGGTCCTGGCTATAAGATGCCTTCGTATAATGCTTTGAGGGGAAAATTGTTGAACCACAGTGTTCATGATGCAGAAGAATATGGAGAGGAACTGAGAAGATCTTGGGATGTGACTGGGTGTTCAGTCATGGTTGATAGGTGGGTGGATAAAGGTGGTCATGCAGTTATCAACTTCTTTGTATACTGCCCCAAGGGCACTGTGTTCCTCAAATCTGCAGATGCGTCAGACATCTCAGAATGTCCAGAAGCACTTCTTAATTTATTTGACGGTGTTGTTCAAGAAGTTGGGCCTCAGAAGATTGTCAATTTTGTGACAGATGCTTCTCCCAGTTGTAAAGCTGCTAGAAAGCTTTTAATGGAGAAATATAAGACCTTTTTCTGCAGTACCTGCGGAGCACATGGTGTTGACTTAATGCTTGAGGAAATTGGGAAAATGAATGAAGTTAAAGAGGTCTTGGCAAAGGCAAAAAAAGTAACTCGATTTGTATACAACAGTGCTTGGATGCTTAATCTGGTAAGGAAGAAAACAGGTGGAAGAGAGATTGTCCAGCTTGCAACCACAAGGTTTGCTTCCGTCTTCCTTACGCTGCAGAATATTGTAGCTCTGAAGCACCAGCTCCAACAAATATTTACCACTGCTGCTTGGATGCAGTCAAATTGCTCTAAGCAAAGTGCTGGACTAGAGGTAGCAGAGATTGTTGCAGACCAACTTTTCTGGTCACTGTGTGATCAAACTCTGAAGGTGACAAAGCACCTACTCTCGGTTTTTCATCTTATGGATTGTGAGGAGAAACCATCTATTGGTTACTTATACGATGCAATTGAAAAGGCAAAGAAAAGTGTAATTGTGGCATTTGACAATAATGAACCTGATTACTTGCCATATTTGAAGGTTATTGATCACGTTTGGCAAGAGGAGCTCCATAGCCCACTTCATGCGGCTGCATGCTATCTCAACCCGTCTATATTTTATAGCCCTAGCTTTTCTACTAACAAAGTCATCCAGAAGGGATTGCTTGACTGTATTGAAACCATAGAGCCTAACTTAACAGCTCAAGTTGTGATTACAAGCAACATAAATTTCTATGAGGAAGCTGTAGGGGATTTTGGTCGACCCGTGGCTTTACGATGTCGAGAATCATTGGCCCCAGCTACTTGGTGGTCCCTGTATGGAGCTGATTACCCTGATCTCCAACGGTTAGCTGTCAGGATACTAAGTCAAACTTGCAGCATTATCCGGTCTAAGAGGAGTGGGAGCATATTTGAACGTATGTATTCAAAGAAAGGGAACCGATTGGAGCAACAGAGGTTGAACGACCTTGTATTTGTTCATCATAACTTGCACCTTCAACATATGTGTTCTGAAGCAAGTAAAGGCGTTTATACAAGAGGTACCCTGGATCCTATATGTTTAGAAGCTATGGATTCCAACATTGAAGATTGGGTGGAAGACCTGGGAGATACTGGCAGCGATGAGTTAAGGTGGATGGATGTAACGGTCCCTGGTGAGTGGAAAGTTATGAATCATAGAGTACAGAGTACCGACGATTGTAATGACAGTACAGATGACAGAGGTAGTGATGATGGGAGAGAATATAATTCTGGGGTGAAAAAGGGAGGGAAAGGGGAGTTCTCACTTGGTACAAGACTTGATGGATCAACCTGGCTTACGAATGTAATGAAGGTCTCCAGCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

768

Amino Acids

85.94

Weight (kDa)

7.55

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22220 AT3G22220 AT3G22220 AT3G22220 AT4G15020 AT4G15020
fragaria_vesca FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_6g39601 FvH4_7g22891 FvH4_7g22891 FvH4_7g22891
malus_domestica MD01G1137200.v1.1 MD02G1265900.v1.1 MD07G1202100.v1.1 MD09G1137900.v1.1 MD09G1138100.v1.1 MD17G1128400.v1.1
prunus_persica Prupe.2G058600_v2.0.a1 Prupe.2G058600_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1 Prupe.3G186900_v2.0.a1
pyrus_communis pycom01g15800 pycom01g15810 pycom01g15840 pycom02g22730 pycom07g18920 pycom07g18930 pycom09g05900 pycom17g12060
rosa_chinensis RchiOBHm_Chr1g0368051 RchiOBHm_Chr2g0153631 RchiOBHm_Chr2g0153641 RchiOBHm_Chr7g0237231
rosa_laevigata RLG00000001022 RLG00000020718
rosa_multiflora Rmu_sc0002117.1_g000006 Rmu_sc0004952.1_g000008 Rmu_sc0005823.1_g000001 Rmu_sc0013273.1_g000001 Rmu_sc0028071.1_g000001
rosa_roxburghii Rroxscaffold_1G00016080 Rroxscaffold_2G00094830 Rroxscaffold_4G00288900
rosa_rugosa Rorug01G0338600 Rorug01G0338700 Rorug02G0441100 Rorug02G0441200 Rorug04G0167900 Rorug07G0273200 Rorug07G0273300 Rorug07G0297100 Rorug07G0297200 Rorug07G0297300
rosa_samantha Rh1AG102500 Rh1AG102600 Rh1AG102700 Rh1AG345600 Rh1BG307900 Rh1CG323500 Rh1DG340000 Rh2AG504100 Rh2BG514800 Rh2CG490100 Rh2DG526900 Rh7AG451500 Rh7AG451700 Rh7AG459100 Rh7AG459200 Rh7BG400700 Rh7BG400800 Rh7BG421300 Rh7BG421400 Rh7BG423400 Rh7BG423500 Rh7BG423800 Rh7BG423900 Rh7CG471000 Rh7CG471100 Rh7CG471200
rosa_wichuraiana Rw1G030760 Rw2G041430 Rw5G036050 Rw7G038020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1001
Acc65I GGTACC 1 cut(s) 1997
AccB1I GGYRCC 1 cut(s) 1997
AccB7I CCANNNNNTGG 1 cut(s) 1406
AccI GTMKAC 4 cut(s) 544, 786, 1092, 1717
AccII CGCG 1 cut(s) 370
AciI CCGC 3 cut(s) 294, 996, 1544
AclWI GGATC 4 cut(s) 41, 2000, 2013, 2269
AcsI RAATTY 4 cut(s) 135, 402, 562, 1684
AcuI CTGAAG 4 cut(s) 222, 1232, 1364, 1989
AdeI CACNNNGTG 3 cut(s) 38, 1007, 1331
AfaI GTAC 8 cut(s) 198, 246, 991, 1999, 2139, 2146, 2167, 2248
AfiI CCNNNNNNNGG 3 cut(s) 310, 1366, 1406
AjnI CCWGG 6 cut(s) 49, 610, 2001, 2058, 2107, 2267
Alw21I GWGCWC 2 cut(s) 1003, 1527
Alw26I GTCTC 1 cut(s) 2296
AlwI GGATC 4 cut(s) 41, 2000, 2013, 2269
AlwNI CAGNNNCTG 2 cut(s) 157, 993
AoxI GGCC 3 cut(s) 57, 893, 1749
ApaI GGGCCC 1 cut(s) 61
ApoI RAATTY 4 cut(s) 135, 402, 562, 1684
ArsI GACNNNNNNTTYG 2 cut(s) 1913, 1945
Asp700I GAANNNNTTC 1 cut(s) 855
Asp718I GGTACC 1 cut(s) 1997
AspA2I CCTAGG 1 cut(s) 232
AspS9I GGNCC 7 cut(s) 57, 58, 608, 893, 1750, 1767, 2104
AsuHPI GGTGA 4 cut(s) 85, 1360, 2123, 2224
AvaII GGWCC 3 cut(s) 608, 1767, 2104
AvrII CCTAGG 1 cut(s) 232
BaeGI GKGCMC 2 cut(s) 61, 803
BamHI GGATCC 1 cut(s) 2005
BanI GGYRCC 1 cut(s) 1997
BanII GRGCYC 2 cut(s) 61, 1527
BauI CACGAG 2 cut(s) 36, 360
BbsI GAAGAC 3 cut(s) 324, 1174, 2061
Bbv12I GWGCWC 2 cut(s) 1003, 1527
BccI CCATC 6 cut(s) 323, 577, 1408, 2086, 2183, 2252
BciT130I CCWGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
BciVI GTATCC 1 cut(s) 1809
BclI TGATCA 2 cut(s) 1333, 1504
BcoDI GTCTC 1 cut(s) 2296
BfaI CTAG 6 cut(s) 233, 282, 948, 1286, 1584, 2302
BfmI CTRYAG 5 cut(s) 393, 819, 985, 1193, 1701
BfuAI ACCTGC 1 cut(s) 1001
BfuI GTATCC 1 cut(s) 1809
BglI GCCNNNNNGGC 1 cut(s) 798
BglII AGATCT 1 cut(s) 704
BlnI CCTAGG 1 cut(s) 232
BlpI GCTNAGC 1 cut(s) 459
BmcAI AGTACT 1 cut(s) 246
Bme1390I CCNGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
Bme18I GGWCC 3 cut(s) 608, 1767, 2104
BmgT120I GGNCC 7 cut(s) 57, 58, 608, 893, 1750, 1767, 2104
BmiI GGNNCC 7 cut(s) 59, 1752, 1769, 1895, 1999, 2007, 2106
BmrFI CCNGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
BmrI ACTGGG 2 cut(s) 729, 927
BmuI ACTGGG 2 cut(s) 729, 927
BpiI GAAGAC 3 cut(s) 324, 1174, 2061
BpmI CTGGAG 1 cut(s) 2278
Bpu1102I GCTNAGC 1 cut(s) 459
BpuEI CTTGAG 4 cut(s) 269, 471, 1043, 1644
BsaI GGTCTC 1 cut(s) 2296
BsaJI CCNNGG 7 cut(s) 61, 232, 795, 1722, 2001, 2059, 2107
BsaWI WCCGGW 1 cut(s) 1842
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 1366, 1406
Bse1I ACTGG 4 cut(s) 304, 724, 933, 2074
Bse3DI GCAATG 1 cut(s) 594
BseBI CCWGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
BseDI CCNNGG 7 cut(s) 61, 232, 795, 1722, 2001, 2059, 2107
BseGI GGATG 7 cut(s) 296, 718, 1113, 1257, 1605, 2097, 2101
BseLI CCNNNNNNNGG 3 cut(s) 310, 1366, 1406
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 4 cut(s) 473, 689, 852, 911
BseNI ACTGG 4 cut(s) 304, 724, 933, 2074
BseRI GAGGAG 3 cut(s) 1406, 1535, 1867
BseSI GKGCMC 2 cut(s) 61, 803
BseYI CCCAGC 2 cut(s) 54, 1753
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 59, 895, 1751
BshNI GGYRCC 1 cut(s) 1997
BsiHKAI GWGCWC 2 cut(s) 1003, 1527
BsiSI CCGG 1 cut(s) 1843
BslFI GGGAC 2 cut(s) 1753, 2090
BslI CCNNNNNNNGG 3 cut(s) 310, 1366, 1406
BsmAI GTCTC 1 cut(s) 2296
BsmFI GGGAC 2 cut(s) 1753, 2090
BsmI GAATGC 1 cut(s) 427
BsnI GGCC 3 cut(s) 59, 895, 1751
Bso31I GGTCTC 1 cut(s) 2296
Bsp120I GGGCCC 1 cut(s) 57
Bsp1286I GDGCHC 4 cut(s) 61, 803, 1003, 1527
Bsp143I GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
Bsp1720I GCTNAGC 1 cut(s) 459
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 3 cut(s) 294, 996, 1544
BspANI GGCC 3 cut(s) 59, 895, 1751
BspCNI CTCAG 4 cut(s) 472, 690, 851, 910
BspFNI CGCG 1 cut(s) 370
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 7 cut(s) 59, 1752, 1769, 1895, 1999, 2007, 2106
BspMAI CTGCAG 3 cut(s) 823, 989, 1197
BspMI ACCTGC 1 cut(s) 1001
BspPI GGATC 4 cut(s) 41, 2000, 2013, 2269
BspT107I GGYRCC 1 cut(s) 1997
BspTNI GGTCTC 1 cut(s) 2296
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 4 cut(s) 304, 724, 933, 2074
BssECI CCNNGG 7 cut(s) 61, 232, 795, 1722, 2001, 2059, 2107
BssMI GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
BssNAI GTATAC 3 cut(s) 545, 787, 1093
BssSI CACGAG 2 cut(s) 36, 360
BssT1I CCWWGG 3 cut(s) 61, 232, 795
Bst1107I GTATAC 3 cut(s) 545, 787, 1093
Bst2BI CACGAG 2 cut(s) 36, 360
Bst2UI CCWGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
Bst4CI ACNGT 9 cut(s) 665, 805, 875, 1100, 1329, 1628, 1803, 2104, 2165
Bst6I CTCTTC 1 cut(s) 1135
BstAPI GCANNNNNTGC 2 cut(s) 108, 993
BstC8I GCNNGC 3 cut(s) 368, 1158, 1552
BstDEI CTNAG 7 cut(s) 459, 698, 838, 897, 1269, 1820, 1848
BstDSI CCRYGG 2 cut(s) 61, 1722
BstF5I GGATG 7 cut(s) 296, 718, 1113, 1257, 1605, 2097, 2101
BstFNI CGCG 1 cut(s) 370
BstKTI GATC 8 cut(s) 49, 502, 707, 1336, 1507, 1795, 2008, 2264
BstMAI GTCTC 1 cut(s) 2296
BstMBI GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
BstMWI GCNNNNNNNGC 9 cut(s) 108, 262, 446, 455, 512, 599, 798, 993, 1522
BstNI CCWGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
BstNSI RCATGY 1 cut(s) 1554
BstSCI CCNGG 6 cut(s) 49, 610, 2001, 2058, 2107, 2267
BstSFI CTRYAG 5 cut(s) 393, 819, 985, 1193, 1701
BstSLI GKGCMC 2 cut(s) 61, 803
BstUI CGCG 1 cut(s) 370
BstV2I GAAGAC 3 cut(s) 324, 1174, 2061
BstX2I RGATCY 2 cut(s) 704, 2005
BstXI CCANNNNNNTGG 1 cut(s) 1761
BstYI RGATCY 2 cut(s) 704, 2005
BstZ17I GTATAC 3 cut(s) 545, 787, 1093
BsuI GTATCC 1 cut(s) 1809
BsuRI GGCC 3 cut(s) 59, 895, 1751
BtgI CCRYGG 2 cut(s) 61, 1722
BtgZI GCGATG 1 cut(s) 2091
BtsCI GGATG 7 cut(s) 296, 718, 1113, 1257, 1605, 2097, 2101
BtsI GCAGTG 1 cut(s) 1239
BtsIMutI CAGTG 5 cut(s) 670, 801, 1105, 1239, 1325
BveI ACCTGC 1 cut(s) 1001
Cac8I GCNNGC 3 cut(s) 368, 1158, 1552
CaiI CAGNNNCTG 2 cut(s) 157, 993
CciI TCATGA 1 cut(s) 670
Cfr13I GGNCC 7 cut(s) 57, 58, 608, 893, 1750, 1767, 2104
CseI GACGC 1 cut(s) 816
Csp6I GTAC 8 cut(s) 197, 245, 990, 1998, 2138, 2145, 2166, 2247
CspCI CAANNNNNGTGG 2 cut(s) 1153, 1188
CviAII CATG 8 cut(s) 62, 186, 671, 733, 764, 1004, 1541, 1551
CviQI GTAC 8 cut(s) 197, 245, 990, 1998, 2138, 2145, 2166, 2247
DdeI CTNAG 7 cut(s) 459, 698, 838, 897, 1269, 1820, 1848
DpnI GATC 8 cut(s) 48, 501, 706, 1335, 1506, 1794, 2007, 2263
DpnII GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
DraI TTTAAA 1 cut(s) 241
DraIII CACNNNGTG 3 cut(s) 38, 1007, 1331
Eam1104I CTCTTC 1 cut(s) 1135
EarI CTCTTC 1 cut(s) 1135
Ecl136II GAGCTC 1 cut(s) 1525
Eco130I CCWWGG 3 cut(s) 61, 232, 795
Eco24I GRGCYC 2 cut(s) 61, 1527
Eco31I GGTCTC 1 cut(s) 2296
Eco47I GGWCC 3 cut(s) 608, 1767, 2104
Eco53kI GAGCTC 1 cut(s) 1525
Eco57I CTGAAG 4 cut(s) 222, 1232, 1364, 1989
EcoICRI GAGCTC 1 cut(s) 1525
EcoRII CCWGG 6 cut(s) 49, 610, 2001, 2058, 2107, 2267
EcoT14I CCWWGG 3 cut(s) 61, 232, 795
EcoT38I GRGCYC 2 cut(s) 61, 1527
ErhI CCWWGG 3 cut(s) 61, 232, 795
FaeI CATG 8 cut(s) 65, 189, 674, 736, 767, 1007, 1544, 1554
FalI AAGNNNNNCTT 8 cut(s) 108, 140, 843, 875, 1159, 1191, 1813, 1845
FaqI GGGAC 2 cut(s) 1753, 2090
FatI CATG 8 cut(s) 61, 185, 670, 732, 763, 1003, 1540, 1550
FauNDI CATATG 1 cut(s) 1959
FbaI TGATCA 2 cut(s) 1333, 1504
FblI GTMKAC 4 cut(s) 544, 786, 1092, 1717
FokI GGATG 7 cut(s) 283, 725, 1120, 1264, 1592, 2104, 2108
FriOI GRGCYC 2 cut(s) 61, 1527
FspBI CTAG 6 cut(s) 233, 282, 948, 1286, 1584, 2302
GsaI CCCAGC 2 cut(s) 58, 1757
GsuI CTGGAG 1 cut(s) 2278
HaeIII GGCC 3 cut(s) 59, 895, 1751
HapII CCGG 1 cut(s) 1843
HgaI GACGC 1 cut(s) 816
Hin1II CATG 8 cut(s) 65, 189, 674, 736, 767, 1007, 1544, 1554
HincII GTYRAC 2 cut(s) 1012, 1718
HindII GTYRAC 2 cut(s) 1012, 1718
HindIII AAGCTT 2 cut(s) 254, 953
HinfI GANTC 6 cut(s) 220, 290, 536, 1742, 2030, 2128
HpaII CCGG 1 cut(s) 1843
HphI GGTGA 4 cut(s) 85, 1360, 2123, 2224
Hpy166II GTNNAC 5 cut(s) 545, 787, 1012, 1093, 1718
Hpy188I TCNGA 9 cut(s) 46, 90, 474, 832, 841, 900, 1212, 1344, 1969
Hpy188III TCNNGA 8 cut(s) 141, 488, 671, 848, 884, 1609, 1739, 1813
Hpy8I GTNNAC 5 cut(s) 545, 787, 1012, 1093, 1718
Hpy99I CGWCG 1 cut(s) 2153
HpyAV CCTTC 8 cut(s) 366, 392, 636, 1339, 1489, 1606, 1961, 2281
HpyCH4III ACNGT 9 cut(s) 665, 805, 875, 1100, 1329, 1628, 1803, 2104, 2165
HpyCH4IV ACGT 2 cut(s) 1509, 1873
HpyF10VI GCNNNNNNNGC 9 cut(s) 108, 262, 446, 455, 512, 599, 798, 993, 1522
HpyF3I CTNAG 7 cut(s) 459, 698, 838, 897, 1269, 1820, 1848
HpySE526I ACGT 2 cut(s) 1509, 1873
Hsp92II CATG 8 cut(s) 65, 189, 674, 736, 767, 1007, 1544, 1554
KpnI GGTACC 1 cut(s) 2001
Ksp22I TGATCA 2 cut(s) 1333, 1504
Kzo9I GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
LmnI GCTCC 8 cut(s) 187, 998, 1227, 1522, 1530, 1778, 1860, 1903
MaeI CTAG 6 cut(s) 233, 282, 948, 1286, 1584, 2302
MaeII ACGT 2 cut(s) 1509, 1873
MaeIII GTNAC 7 cut(s) 715, 916, 1078, 1323, 1348, 1409, 2098
MalI GATC 8 cut(s) 48, 501, 706, 1335, 1506, 1794, 2007, 2263
MboI GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
MfeI CAATTG 1 cut(s) 1425
MflI RGATCY 2 cut(s) 704, 2005
MhlI GDGCHC 4 cut(s) 61, 803, 1003, 1527
MmeI TCCRAC 3 cut(s) 1249, 1822, 2058
MroXI GAANNNNTTC 1 cut(s) 855
MslI CAYNNNNRTG 1 cut(s) 71
MspI CCGG 1 cut(s) 1843
MspR9I CCNGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
MunI CAATTG 1 cut(s) 1425
Mva1269I GAATGC 1 cut(s) 427
MvaI CCWGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 9 cut(s) 108, 262, 446, 455, 512, 599, 798, 993, 1522
NcoI CCATGG 1 cut(s) 61
NdeI CATATG 1 cut(s) 1959
NdeII GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
NlaIII CATG 8 cut(s) 65, 189, 674, 736, 767, 1007, 1544, 1554
NlaIV GGNNCC 7 cut(s) 59, 1752, 1769, 1895, 1999, 2007, 2106
NmuCI GTSAC 4 cut(s) 715, 916, 1323, 1348
NspI RCATGY 1 cut(s) 1554
PaeI GCATGC 1 cut(s) 1554
PagI TCATGA 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PctI GAATGC 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 855
PfeI GAWTC 6 cut(s) 220, 290, 536, 1742, 2030, 2128
PflMI CCANNNNNTGG 1 cut(s) 1406
Psp124BI GAGCTC 1 cut(s) 1527
Psp6I CCWGG 6 cut(s) 49, 610, 2001, 2058, 2107, 2267
PspFI CCCAGC 2 cut(s) 54, 1753
PspGI CCWGG 6 cut(s) 49, 610, 2001, 2058, 2107, 2267
PspN4I GGNNCC 7 cut(s) 59, 1752, 1769, 1895, 1999, 2007, 2106
PspOMI GGGCCC 1 cut(s) 57
PspPI GGNCC 7 cut(s) 57, 58, 608, 893, 1750, 1767, 2104
PstI CTGCAG 3 cut(s) 823, 989, 1197
PstNI CAGNNNCTG 2 cut(s) 157, 993
PsuI RGATCY 2 cut(s) 704, 2005
RsaI GTAC 8 cut(s) 198, 246, 991, 1999, 2139, 2146, 2167, 2248
RsaNI GTAC 8 cut(s) 197, 245, 990, 1998, 2138, 2145, 2166, 2247
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 1527
SalI GTCGAC 1 cut(s) 1716
Sau3AI GATC 8 cut(s) 46, 499, 704, 1333, 1504, 1792, 2005, 2261
Sau96I GGNCC 7 cut(s) 57, 58, 608, 893, 1750, 1767, 2104
ScaI AGTACT 1 cut(s) 246
ScrFI CCNGG 6 cut(s) 51, 612, 2003, 2060, 2109, 2269
SduI GDGCHC 4 cut(s) 61, 803, 1003, 1527
SfcI CTRYAG 5 cut(s) 393, 819, 985, 1193, 1701
SinI GGWCC 3 cut(s) 608, 1767, 2104
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 4 cut(s) 248, 486, 1022, 1659
SmoI CTYRAG 4 cut(s) 248, 486, 1022, 1659
SphI GCATGC 1 cut(s) 1554
SsiI CCGC 3 cut(s) 294, 996, 1544
SspI AATATT 2 cut(s) 1201, 1233
SspMI CTAG 6 cut(s) 233, 282, 948, 1286, 1584, 2302
SstI GAGCTC 1 cut(s) 1527
StyD4I CCNGG 6 cut(s) 49, 610, 2001, 2058, 2107, 2267
StyI CCWWGG 3 cut(s) 61, 232, 795
TaaI ACNGT 9 cut(s) 665, 805, 875, 1100, 1329, 1628, 1803, 2104, 2165
TaiI ACGT 2 cut(s) 1512, 1876
TaqI TCGA 3 cut(s) 1084, 1717, 1738
TaqII GACCGA 1 cut(s) 596
TatI WGTACW 3 cut(s) 244, 2137, 2165
TauI GCSGC 1 cut(s) 1547
TfiI GAWTC 6 cut(s) 220, 290, 536, 1742, 2030, 2128
TscAI CASTG 5 cut(s) 670, 808, 1105, 1246, 1332
TseFI GTSAC 4 cut(s) 715, 916, 1323, 1348
Tsp45I GTSAC 4 cut(s) 715, 916, 1323, 1348
TspGWI ACGGA 1 cut(s) 1168
TspRI CASTG 5 cut(s) 670, 808, 1105, 1246, 1332
Van91I CCANNNNNTGG 1 cut(s) 1406
VpaK11BI GGWCC 3 cut(s) 608, 1767, 2104
XapI RAATTY 4 cut(s) 135, 402, 562, 1684
XceI RCATGY 1 cut(s) 1554
XcmI CCANNNNNNNNNTGG 1 cut(s) 1761
XmaJI CCTAGG 1 cut(s) 232
XmiI GTMKAC 4 cut(s) 544, 786, 1092, 1717
XmnI GAANNNNTTC 1 cut(s) 855
XspI CTAG 6 cut(s) 233, 282, 948, 1286, 1584, 2302
ZrmI AGTACT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.