Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000625

Physical Location & Seq

Genomic Coordinates Reverse (-)

7067867 .. 7071218

Gene Structure 3352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1089300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 930 bp

ATGAAGCGAATGACCCTCGCCGAAAAGATTGGTCAAATGACGCAGATCGAGCAAACTGTTGCAACCCCTGATGGAATGTTTCTTGGAGGCCAAGAAATTGCGGTGAAAAGGCTATCAAGGGTTTCAGGACAAGGGTTACAGGAATTTAGAAATGAGGTGGTGCTGATCGCCGAAAAGATTGGTCAAATGACGCAGATCAAGCAAACTGTTGCAACCCCTGATGTCATGAACAAGTATTTTATTGGGAGTGTGCTTAGTGGTGGTGGGAATGTACCAGCCCCAAAAGCTTCAGCTGAGGCATGGGTCAATCTGGTGAATGGCCTCCAAAAGGGATCTCTATCTACCCGTCTTGGGATTCCTATGATCTATGGGATTGATGCGGTTCACGGCCACAACAACGTCTACAATGCTACTATTTTCCCTCATAATGTTGGACTAGATCCTAATCTTGTTAAGAGAATTGGGGAAGCAACTGCCCTAGAAGTCAGGGCAACCGGAATTCCTTATGTCTTTGCCCCATGTATTGCGGTCTGCAGAGATCCGAGATGGGGTAGGTGCTACGAAAGCTATAGTGAAGACCATAAGATTGTTCAAGCAATGACTGAGATAATACCTGGTCTGCAAGGAGATATGCCTCCCACTGCTCGAAAGGGGGCTCCCTATGTTTCTACTGCAAATTATGGTGCTTACTCGAGTTGGAATGGAAACAAAAGGCATGCAAATAAAGAACTTGTAACCGGATACCTTAAGGACAAGCTACGACGTTTCAGGGGTTTTGTCATATCAGATTGGGAGGGTATTGACAGGATTACATCTCCTCCCAAAGCTAACTATTCATATTCAGTTCAAGCTGGAGTTGGTGCTGGAATCGACATGGTTAGTCTAATTAAAAGTGTAACTTACCGAATGTATTTTTTTGGTGAATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005623 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0042597 GO:0043170 GO:0044042 GO:0044238 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

310

Amino Acids

33.92

Weight (kDa)

9.28

Isoelectric Point (pI)

31.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	56 - 210	1.8e-28	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3	PF00933	229 - 295	8.1e-07	Glycosyl hydrolase family 3 N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47010 AT3G47040 AT3G47050
fragaria_vesca	FvH4_1g28352 FvH4_3g26960 FvH4_7g05180
malus_domestica	MD02G1089300.v1.1 MD02G1259900.v1.1 MD14G1004400.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G092700_v2.0.a1 Prupe.7G007100_v2.0.a1 Prupe.7G007700_v2.0.a1
pyrus_communis	pycom02g22100 pycom07g04670 pycom11g06820 pycom11g15190 pycom14g00200 pycom14g00230
rosa_chinensis	RchiOBHm_Chr5g0049431 RchiOBHm_Chr5g0049441 RchiOBHm_Chr5g0079371 RchiOBHm_Chr6g0274311
rosa_laevigata	RLG00000006403 RLG00000008403 RLG00000028847 RLG00000034618
rosa_multiflora	Rmu_co8385859.1_g000001 Rmu_sc0000376.1_g000001 Rmu_sc0001307.1_g000017 Rmu_sc0001307.1_g000019 Rmu_sc0001900.1_g000031 Rmu_sc0002912.1_g000028 Rmu_sc0006084.1_g000018 Rmu_sc0006888.1_g000063 Rmu_sc0014052.1_g000002 Rmu_sc0014052.1_g000004 Rmu_sc0022931.1_g000004
rosa_roxburghii	Rroxscaffold_1G00003570 Rroxscaffold_1G00032240 Rroxscaffold_1G00032250 Rroxscaffold_2G00103090 Rroxscaffold_4G00289090 Rroxscaffold_5G00336970
rosa_rugosa	Rorug03G0185900 Rorug04G0020500 Rorug04G0020600 Rorug04G0020600 Rorug04G0020700.1 Rorug04G0020800.1 Rorug04G0020900 Rorug04G0020900 Rorug04G0020900 Rorug05G0248500 Rorug05G0248500 Rorug05G0248500 Rorug05G0248600 Rorug05G0465800
rosa_samantha	Rh3CG363400 Rh4DG330500 Rh4DG330600 Rh5AG245600 Rh5AG516800 Rh5AG517600 Rh5AG519000 Rh5AG519500 Rh5BG335900 Rh5BG336000 Rh5BG540700 Rh5BG542700 Rh5DG348200 Rh5DG348300 Rh5DG551900 Rh6BG185500 Rh7DG265300
rosa_wichuraiana	Rw1G018240 Rw4G028270 Rw5G030690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	402
AciI	CCGC	3 cut(s)	101, 380, 527
AclWI	GGATC	3 cut(s)	340, 434, 533
AcoI	YGGCCR	1 cut(s)	388
AcsI	RAATTY	3 cut(s)	143, 498, 923
AcuI	CTGAAG	1 cut(s)	273
AfaI	GTAC	1 cut(s)	273
AfiI	CCNNNNNNNGG	3 cut(s)	328, 351, 548
AflII	CTTAAG	1 cut(s)	746
AgsI	TTSAA	2 cut(s)	593, 848
AjnI	CCWGG	1 cut(s)	613
AluBI	AGCT	6 cut(s)	287, 293, 567, 757, 827, 851
AluI	AGCT	6 cut(s)	287, 293, 567, 757, 827, 851
AlwI	GGATC	3 cut(s)	340, 434, 533
Ama87I	CYCGRG	1 cut(s)	691
AoxI	GGCC	3 cut(s)	88, 319, 388
ApoI	RAATTY	3 cut(s)	143, 498, 923
AsuHPI	GGTGA	2 cut(s)	115, 325
AvaI	CYCGRG	1 cut(s)	691
BanII	GRGCYC	1 cut(s)	658
BbsI	GAAGAC	1 cut(s)	582
BbvCI	CCTCAGC	1 cut(s)	294
BccI	CCATC	2 cut(s)	65, 540
BceAI	ACGGC	1 cut(s)	403
BciT130I	CCWGG	1 cut(s)	615
BciVI	GTATCC	1 cut(s)	734
BfaI	CTAG	2 cut(s)	437, 479
BfmI	CTRYAG	2 cut(s)	532, 568
BfrI	CTTAAG	1 cut(s)	746
BfuI	GTATCC	1 cut(s)	734
Bme1390I	CCNGG	1 cut(s)	615
BmeT110I	CYCGRG	1 cut(s)	691
BmiI	GGNNCC	1 cut(s)	657
BmrFI	CCNGG	1 cut(s)	615
BmsI	GCATC	1 cut(s)	367
BpiI	GAAGAC	1 cut(s)	582
BpmI	CTGGAG	1 cut(s)	873
Bpu10I	CCTNAGC	1 cut(s)	294
BsaBI	GATNNNNATC	2 cut(s)	337, 444
BsaWI	WCCGGW	2 cut(s)	494, 737
BsaXI	ACNNNNNCTCC	2 cut(s)	802, 832
Bsc4I	CCNNNNNNNGG	3 cut(s)	328, 351, 548
Bse3DI	GCAATG	1 cut(s)	603
Bse8I	GATNNNNATC	2 cut(s)	337, 444
BseBI	CCWGG	1 cut(s)	615
BseJI	GATNNNNATC	2 cut(s)	337, 444
BseLI	CCNNNNNNNGG	3 cut(s)	328, 351, 548
BseMI	GCAATG	1 cut(s)	603
BseMII	CTCAG	2 cut(s)	285, 594
BseRI	GAGGAG	1 cut(s)	807
BshFI	GGCC	3 cut(s)	90, 321, 390
BsiHKCI	CYCGRG	1 cut(s)	691
BsiSI	CCGG	2 cut(s)	495, 738
BslI	CCNNNNNNNGG	3 cut(s)	328, 351, 548
BsnI	GGCC	3 cut(s)	90, 321, 390
BsoBI	CYCGRG	1 cut(s)	691
Bsp1286I	GDGCHC	1 cut(s)	658
Bsp143I	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
BspACI	CCGC	3 cut(s)	101, 380, 527
BspANI	GGCC	3 cut(s)	90, 321, 390
BspCNI	CTCAG	2 cut(s)	286, 595
BspHI	TCATGA	1 cut(s)	225
BspLI	GGNNCC	1 cut(s)	657
BspMAI	CTGCAG	1 cut(s)	536
BspPI	GGATC	3 cut(s)	340, 434, 533
BspTI	CTTAAG	1 cut(s)	746
BsrDI	GCAATG	1 cut(s)	603
BssMI	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
Bst2UI	CCWGG	1 cut(s)	615
Bst4CI	ACNGT	2 cut(s)	58, 208
BstAFI	CTTAAG	1 cut(s)	746
BstC8I	GCNNGC	1 cut(s)	717
BstDEI	CTNAG	3 cut(s)	254, 294, 603
BstENI	CCTNNNNNAGG	1 cut(s)	326
BstKTI	GATC	7 cut(s)	48, 168, 198, 335, 366, 442, 541
BstMBI	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
BstMWI	GCNNNNNNNGC	4 cut(s)	49, 199, 284, 564
BstNI	CCWGG	1 cut(s)	615
BstNSI	RCATGY	1 cut(s)	719
BstSCI	CCNGG	1 cut(s)	613
BstSFI	CTRYAG	2 cut(s)	532, 568
BstV2I	GAAGAC	1 cut(s)	582
BstX2I	RGATCY	3 cut(s)	332, 439, 538
BstYI	RGATCY	3 cut(s)	332, 439, 538
BsuI	GTATCC	1 cut(s)	734
BsuRI	GGCC	3 cut(s)	90, 321, 390
BtsI	GCAGTG	1 cut(s)	639
BtsIMutI	CAGTG	1 cut(s)	639
Cac8I	GCNNGC	1 cut(s)	717
CciI	TCATGA	1 cut(s)	225
CseI	GACGC	2 cut(s)	49, 199
CsiI	ACCWGGT	1 cut(s)	613
Csp6I	GTAC	1 cut(s)	272
CviAII	CATG	5 cut(s)	226, 300, 519, 716, 874
CviQI	GTAC	1 cut(s)	272
DdeI	CTNAG	3 cut(s)	254, 294, 603
DpnI	GATC	7 cut(s)	47, 167, 197, 334, 365, 441, 540
DpnII	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
EaeI	YGGCCR	1 cut(s)	388
Eco24I	GRGCYC	1 cut(s)	658
Eco57I	CTGAAG	1 cut(s)	273
Eco88I	CYCGRG	1 cut(s)	691
EcoNI	CCTNNNNNAGG	1 cut(s)	326
EcoRI	GAATTC	1 cut(s)	498
EcoRII	CCWGG	1 cut(s)	613
EcoT38I	GRGCYC	1 cut(s)	658
FaeI	CATG	5 cut(s)	229, 303, 522, 719, 877
FalI	AAGNNNNNCTT	2 cut(s)	883, 915
FatI	CATG	5 cut(s)	225, 299, 518, 715, 873
FblI	GTMKAC	1 cut(s)	402
FriOI	GRGCYC	1 cut(s)	658
FspBI	CTAG	2 cut(s)	437, 479
GsuI	CTGGAG	1 cut(s)	873
HaeIII	GGCC	3 cut(s)	90, 321, 390
HapII	CCGG	2 cut(s)	495, 738
HgaI	GACGC	2 cut(s)	49, 199
Hin1II	CATG	5 cut(s)	229, 303, 522, 719, 877
HindIII	AAGCTT	1 cut(s)	285
HinfI	GANTC	2 cut(s)	355, 867
HpaII	CCGG	2 cut(s)	495, 738
HphI	GGTGA	2 cut(s)	115, 325
Hpy166II	GTNNAC	2 cut(s)	385, 403
Hpy188I	TCNGA	2 cut(s)	543, 787
Hpy188III	TCNNGA	2 cut(s)	126, 226
Hpy8I	GTNNAC	2 cut(s)	385, 403
Hpy99I	CGWCG	1 cut(s)	765
HpyCH4III	ACNGT	2 cut(s)	58, 208
HpyCH4IV	ACGT	2 cut(s)	399, 763
HpyCH4V	TGCA	6 cut(s)	62, 212, 534, 622, 674, 719
HpyF10VI	GCNNNNNNNGC	4 cut(s)	49, 199, 284, 564
HpyF3I	CTNAG	3 cut(s)	254, 294, 603
HpySE526I	ACGT	2 cut(s)	399, 763
Hsp92II	CATG	5 cut(s)	229, 303, 522, 719, 877
Kzo9I	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
LmnI	GCTCC	1 cut(s)	661
LweI	GCATC	1 cut(s)	367
MabI	ACCWGGT	1 cut(s)	613
MaeI	CTAG	2 cut(s)	437, 479
MaeII	ACGT	2 cut(s)	399, 763
MaeIII	GTNAC	3 cut(s)	135, 733, 895
MalI	GATC	7 cut(s)	47, 167, 197, 334, 365, 441, 540
MboI	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
MboII	GAAGA	1 cut(s)	587
MflI	RGATCY	3 cut(s)	332, 439, 538
MhlI	GDGCHC	1 cut(s)	658
MluCI	AATT	7 cut(s)	96, 143, 459, 498, 676, 885, 923
MmeI	TCCRAC	2 cut(s)	412, 677
MnlI	CCTC	9 cut(s)	26, 80, 148, 289, 332, 432, 645, 787, 828
MseI	TTAA	3 cut(s)	453, 747, 888
MspA1I	CMGCKG	1 cut(s)	293
MspCI	CTTAAG	1 cut(s)	746
MspI	CCGG	2 cut(s)	495, 738
MspR9I	CCNGG	1 cut(s)	615
MvaI	CCWGG	1 cut(s)	615
MwoI	GCNNNNNNNGC	4 cut(s)	49, 199, 284, 564
NdeII	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
NlaIII	CATG	5 cut(s)	229, 303, 522, 719, 877
NlaIV	GGNNCC	1 cut(s)	657
NspI	RCATGY	1 cut(s)	719
PaeI	GCATGC	1 cut(s)	719
PaeR7I	CTCGAG	1 cut(s)	691
PagI	TCATGA	1 cut(s)	225
PfeI	GAWTC	2 cut(s)	355, 867
Psp6I	CCWGG	1 cut(s)	613
PspGI	CCWGG	1 cut(s)	613
PspN4I	GGNNCC	1 cut(s)	657
PspXI	VCTCGAGB	1 cut(s)	691
PstI	CTGCAG	1 cut(s)	536
PsuI	RGATCY	3 cut(s)	332, 439, 538
PvuII	CAGCTG	1 cut(s)	293
RsaI	GTAC	1 cut(s)	273
RsaNI	GTAC	1 cut(s)	272
SaqAI	TTAA	3 cut(s)	453, 747, 888
Sau3AI	GATC	7 cut(s)	45, 165, 195, 332, 363, 439, 538
ScrFI	CCNGG	1 cut(s)	615
SduI	GDGCHC	1 cut(s)	658
SexAI	ACCWGGT	1 cut(s)	613
SfaNI	GCATC	1 cut(s)	367
SfcI	CTRYAG	2 cut(s)	532, 568
Sfr274I	CTCGAG	1 cut(s)	691
SlaI	CTCGAG	1 cut(s)	691
SmlI	CTYRAG	2 cut(s)	691, 746
SmoI	CTYRAG	2 cut(s)	691, 746
SphI	GCATGC	1 cut(s)	719
Sse9I	AATT	7 cut(s)	96, 143, 459, 498, 676, 885, 923
SsiI	CCGC	3 cut(s)	101, 380, 527
SspMI	CTAG	2 cut(s)	437, 479
StyD4I	CCNGG	1 cut(s)	613
TaaI	ACNGT	2 cut(s)	58, 208
TaiI	ACGT	2 cut(s)	402, 766
TaqI	TCGA	4 cut(s)	48, 646, 692, 870
TasI	AATT	7 cut(s)	96, 143, 459, 498, 676, 885, 923
TfiI	GAWTC	2 cut(s)	355, 867
Tru1I	TTAA	3 cut(s)	453, 747, 888
Tru9I	TTAA	3 cut(s)	453, 747, 888
TscAI	CASTG	1 cut(s)	646
TspDTI	ATGAA	3 cut(s)	17, 242, 825
TspRI	CASTG	1 cut(s)	646
Vha464I	CTTAAG	1 cut(s)	746
XagI	CCTNNNNNAGG	1 cut(s)	326
XapI	RAATTY	3 cut(s)	143, 498, 923
XceI	RCATGY	1 cut(s)	719
XhoI	CTCGAG	1 cut(s)	691
XmiI	GTMKAC	1 cut(s)	402
XspI	CTAG	2 cut(s)	437, 479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.