Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000625

Physical Location & Seq

Genomic Coordinates Reverse (-)

483738 .. 485575

Gene Structure 1838 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1004400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 636 bp

ATGGCTAGGAATCCCTTCTTGTTGGGGTTTCTTTTAGTTTTCTGCATAGCTATTGCAGAGGCAGAATACATTAAATATAAAGATCCCAAACAACCATTGAATTCTCGAATCAAGGATCTACTGAGCCGGATGACGTTGGAGGAAAAGATTGGCCAAATGGTGCAGATAGACCGCAGCGTTGCTTCGCCTGAGGTGATGAAGAAGCACTTCATTGGAAGTATATTAAGAGGGGTTGTGAATGTTCCGCCACAAAAGGATTCTGCAGAAACTTGGATTAACATGGTGAATGATCTTCAGAAGGGTTCTTTATCAACTCGGCTTGGAATTCCGTTTATTTTTGGTATCGATGCTGTTCATGGCCACAATAATGTTTACAAAGCAACAATCTTTCCTCACAATGTTGGCCTTGGAGCAACCAGGCAAGTAGATGTCTGCAGAGATCCAAGATGGGGTCGATGTTATGAAAGCTACAGGGTGGCTGATGTTTTATTTGGTGACTACGGCTTCACTGGCAAGCTTTCTAGAACTTGGTTCAAAACGGTTGATCAACTACCTATGAATGTTGGGGATGCACATTATGACCCTCTCTTCCCCTTTGGATTTGGGCTCACTACAACACCCAAAAAATTCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0042597 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

212

Amino Acids

23.86

Weight (kDa)

9.24

Isoelectric Point (pI)

30.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	45 - 147	1.1e-14	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	158 - 204	1.8e-07	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47010 AT3G47040 AT3G47050
fragaria_vesca	FvH4_1g28352 FvH4_3g26960 FvH4_7g05180
malus_domestica	MD02G1089300.v1.1 MD02G1259900.v1.1 MD14G1004400.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G092700_v2.0.a1 Prupe.7G007100_v2.0.a1 Prupe.7G007700_v2.0.a1
pyrus_communis	pycom02g22100 pycom07g04670 pycom11g06820 pycom11g15190 pycom14g00200 pycom14g00230
rosa_chinensis	RchiOBHm_Chr5g0049431 RchiOBHm_Chr5g0049441 RchiOBHm_Chr5g0079371 RchiOBHm_Chr6g0274311
rosa_laevigata	RLG00000006403 RLG00000008403 RLG00000028847 RLG00000034618
rosa_multiflora	Rmu_co8385859.1_g000001 Rmu_sc0000376.1_g000001 Rmu_sc0001307.1_g000017 Rmu_sc0001307.1_g000019 Rmu_sc0001900.1_g000031 Rmu_sc0002912.1_g000028 Rmu_sc0006084.1_g000018 Rmu_sc0006888.1_g000063 Rmu_sc0014052.1_g000002 Rmu_sc0014052.1_g000004 Rmu_sc0022931.1_g000004
rosa_roxburghii	Rroxscaffold_1G00003570 Rroxscaffold_1G00032240 Rroxscaffold_1G00032250 Rroxscaffold_2G00103090 Rroxscaffold_4G00289090 Rroxscaffold_5G00336970
rosa_rugosa	Rorug03G0185900 Rorug04G0020500 Rorug04G0020600 Rorug04G0020600 Rorug04G0020700.1 Rorug04G0020800.1 Rorug04G0020900 Rorug04G0020900 Rorug04G0020900 Rorug05G0248500 Rorug05G0248500 Rorug05G0248500 Rorug05G0248600 Rorug05G0465800
rosa_samantha	Rh3CG363400 Rh4DG330500 Rh4DG330600 Rh5AG245600 Rh5AG516800 Rh5AG517600 Rh5AG519000 Rh5AG519500 Rh5BG335900 Rh5BG336000 Rh5BG540700 Rh5BG542700 Rh5DG348200 Rh5DG348300 Rh5DG551900 Rh6BG185500 Rh7DG265300
rosa_wichuraiana	Rw1G018240 Rw4G028270 Rw5G030690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	172, 245
AclWI	GGATC	3 cut(s)	77, 123, 434
AcoI	YGGCCR	2 cut(s)	151, 358
AcsI	RAATTY	3 cut(s)	100, 324, 626
AcuI	CTGAAG	1 cut(s)	278
AfiI	CCNNNNNNNGG	1 cut(s)	449
AgsI	TTSAA	3 cut(s)	100, 535, 631
AjnI	CCWGG	1 cut(s)	416
AluBI	AGCT	3 cut(s)	50, 468, 517
AluI	AGCT	3 cut(s)	50, 468, 517
AlwI	GGATC	3 cut(s)	77, 123, 434
AoxI	GGCC	3 cut(s)	151, 358, 403
ApeKI	GCWGC	1 cut(s)	174
ApoI	RAATTY	3 cut(s)	100, 324, 626
Asp700I	GAANNNNTTC	2 cut(s)	14, 206
AsuHPI	GGTGA	3 cut(s)	205, 295, 506
AxyI	CCTNAGG	1 cut(s)	189
BalI	TGGCCA	2 cut(s)	153, 360
BanII	GRGCYC	1 cut(s)	609
BbvI	GCAGC	1 cut(s)	186
BccI	CCATC	1 cut(s)	441
BceAI	ACGGC	1 cut(s)	517
BciT130I	CCWGG	1 cut(s)	418
BclI	TGATCA	1 cut(s)	544
BfaI	CTAG	3 cut(s)	6, 522, 634
BfmI	CTRYAG	3 cut(s)	261, 433, 469
BisI	GCNGC	1 cut(s)	175
BlsI	GCNGC	1 cut(s)	176
Bme1390I	CCNGG	1 cut(s)	418
BmrFI	CCNGG	1 cut(s)	418
BmsI	GCATC	2 cut(s)	337, 559
Bsa29I	ATCGAT	1 cut(s)	345
BsaJI	CCNNGG	1 cut(s)	406
Bsc4I	CCNNNNNNNGG	1 cut(s)	449
Bse1I	ACTGG	1 cut(s)	514
Bse21I	CCTNAGG	1 cut(s)	189
BseBI	CCWGG	1 cut(s)	418
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	1 cut(s)	406
BseGI	GGATG	2 cut(s)	135, 574
BseLI	CCNNNNNNNGG	1 cut(s)	449
BseMII	CTCAG	2 cut(s)	113, 180
BseNI	ACTGG	1 cut(s)	514
BseXI	GCAGC	1 cut(s)	186
BsgI	GTGCAG	1 cut(s)	182
BshFI	GGCC	3 cut(s)	153, 360, 405
BshVI	ATCGAT	1 cut(s)	345
BsiSI	CCGG	1 cut(s)	127
BslI	CCNNNNNNNGG	1 cut(s)	449
BsnI	GGCC	3 cut(s)	153, 360, 405
Bsp1286I	GDGCHC	1 cut(s)	609
Bsp143I	GATC	5 cut(s)	82, 115, 289, 439, 544
BspACI	CCGC	2 cut(s)	172, 245
BspANI	GGCC	3 cut(s)	153, 360, 405
BspCNI	CTCAG	2 cut(s)	114, 181
BspDI	ATCGAT	1 cut(s)	345
BspMAI	CTGCAG	2 cut(s)	265, 437
BspPI	GGATC	3 cut(s)	77, 123, 434
BsrI	ACTGG	1 cut(s)	514
BssECI	CCNNGG	1 cut(s)	406
BssMI	GATC	5 cut(s)	82, 115, 289, 439, 544
BssT1I	CCWWGG	1 cut(s)	406
Bst2UI	CCWGG	1 cut(s)	418
Bst4CI	ACNGT	1 cut(s)	541
Bst6I	CTCTTC	1 cut(s)	593
BstC8I	GCNNGC	1 cut(s)	515
BstDEI	CTNAG	2 cut(s)	122, 189
BstF5I	GGATG	2 cut(s)	135, 574
BstKTI	GATC	5 cut(s)	85, 118, 292, 442, 547
BstMBI	GATC	5 cut(s)	82, 115, 289, 439, 544
BstMWI	GCNNNNNNNGC	1 cut(s)	510
BstNI	CCWGG	1 cut(s)	418
BstSCI	CCNGG	1 cut(s)	416
BstSFI	CTRYAG	3 cut(s)	261, 433, 469
BstV1I	GCAGC	1 cut(s)	186
BstX2I	RGATCY	3 cut(s)	82, 115, 439
BstYI	RGATCY	3 cut(s)	82, 115, 439
Bsu15I	ATCGAT	1 cut(s)	345
Bsu36I	CCTNAGG	1 cut(s)	189
BsuRI	GGCC	3 cut(s)	153, 360, 405
BsuTUI	ATCGAT	1 cut(s)	345
BtsCI	GGATG	2 cut(s)	135, 574
BtsIMutI	CAGTG	1 cut(s)	507
Cac8I	GCNNGC	1 cut(s)	515
ClaI	ATCGAT	1 cut(s)	345
CviAII	CATG	2 cut(s)	280, 356
DdeI	CTNAG	2 cut(s)	122, 189
DpnI	GATC	5 cut(s)	84, 117, 291, 441, 546
DpnII	GATC	5 cut(s)	82, 115, 289, 439, 544
EaeI	YGGCCR	2 cut(s)	151, 358
Eam1104I	CTCTTC	1 cut(s)	593
EarI	CTCTTC	1 cut(s)	593
EciI	GGCGGA	1 cut(s)	234
Eco130I	CCWWGG	1 cut(s)	406
Eco24I	GRGCYC	1 cut(s)	609
Eco57I	CTGAAG	1 cut(s)	278
Eco81I	CCTNAGG	1 cut(s)	189
EcoRI	GAATTC	2 cut(s)	100, 324
EcoRII	CCWGG	1 cut(s)	416
EcoT14I	CCWWGG	1 cut(s)	406
EcoT38I	GRGCYC	1 cut(s)	609
ErhI	CCWWGG	1 cut(s)	406
FaeI	CATG	2 cut(s)	283, 359
FaiI	YATR	8 cut(s)	47, 78, 221, 281, 357, 462, 557, 579
FalI	AAGNNNNNCTT	2 cut(s)	191, 223
FatI	CATG	2 cut(s)	279, 355
FbaI	TGATCA	1 cut(s)	544
Fnu4HI	GCNGC	1 cut(s)	175
FokI	GGATG	2 cut(s)	142, 581
FriOI	GRGCYC	1 cut(s)	609
Fsp4HI	GCNGC	1 cut(s)	175
FspBI	CTAG	3 cut(s)	6, 522, 634
GluI	GCNGC	1 cut(s)	175
HaeIII	GGCC	3 cut(s)	153, 360, 405
HapII	CCGG	1 cut(s)	127
Hin1II	CATG	2 cut(s)	283, 359
HindIII	AAGCTT	1 cut(s)	515
HinfI	GANTC	3 cut(s)	10, 108, 257
HpaII	CCGG	1 cut(s)	127
HphI	GGTGA	3 cut(s)	205, 295, 506
Hpy166II	GTNNAC	1 cut(s)	373
Hpy188I	TCNGA	1 cut(s)	297
Hpy188III	TCNNGA	2 cut(s)	105, 522
Hpy8I	GTNNAC	1 cut(s)	373
HpyAV	CCTTC	2 cut(s)	25, 292
HpyCH4III	ACNGT	1 cut(s)	541
HpyCH4IV	ACGT	1 cut(s)	134
HpyCH4V	TGCA	6 cut(s)	45, 56, 163, 263, 435, 572
HpyF10VI	GCNNNNNNNGC	1 cut(s)	510
HpyF3I	CTNAG	2 cut(s)	122, 189
HpySE526I	ACGT	1 cut(s)	134
Hsp92II	CATG	2 cut(s)	283, 359
Ksp22I	TGATCA	1 cut(s)	544
Kzo9I	GATC	5 cut(s)	82, 115, 289, 439, 544
LmnI	GCTCC	1 cut(s)	410
LpnPI	CCDG	6 cut(s)	140, 201, 403, 430, 457, 495
Lsp1109I	GCAGC	1 cut(s)	186
LweI	GCATC	2 cut(s)	337, 559
MaeI	CTAG	3 cut(s)	6, 522, 634
MaeII	ACGT	1 cut(s)	134
MaeIII	GTNAC	1 cut(s)	494
MalI	GATC	5 cut(s)	84, 117, 291, 441, 546
MboI	GATC	5 cut(s)	82, 115, 289, 439, 544
MboII	GAAGA	3 cut(s)	211, 284, 580
MflI	RGATCY	3 cut(s)	82, 115, 439
MhlI	GDGCHC	1 cut(s)	609
MlsI	TGGCCA	2 cut(s)	153, 360
MluCI	AATT	3 cut(s)	100, 324, 626
MluNI	TGGCCA	2 cut(s)	153, 360
MmeI	TCCRAC	1 cut(s)	117
MnlI	CCTC	6 cut(s)	52, 133, 184, 221, 402, 594
Mox20I	TGGCCA	2 cut(s)	153, 360
MroXI	GAANNNNTTC	2 cut(s)	14, 206
MscI	TGGCCA	2 cut(s)	153, 360
MseI	TTAA	3 cut(s)	72, 224, 276
MslI	CAYNNNNRTG	1 cut(s)	366
Msp20I	TGGCCA	2 cut(s)	153, 360
MspI	CCGG	1 cut(s)	127
MspR9I	CCNGG	1 cut(s)	418
MvaI	CCWGG	1 cut(s)	418
MwoI	GCNNNNNNNGC	1 cut(s)	510
NdeII	GATC	5 cut(s)	82, 115, 289, 439, 544
NlaIII	CATG	2 cut(s)	283, 359
NmeAIII	GCCGAG	1 cut(s)	295
NmuCI	GTSAC	1 cut(s)	494
PdmI	GAANNNNTTC	2 cut(s)	14, 206
PfeI	GAWTC	3 cut(s)	10, 108, 257
PkrI	GCNGC	1 cut(s)	176
Psp6I	CCWGG	1 cut(s)	416
PspGI	CCWGG	1 cut(s)	416
PstI	CTGCAG	2 cut(s)	265, 437
PsuI	RGATCY	3 cut(s)	82, 115, 439
RseI	CAYNNNNRTG	1 cut(s)	366
SaqAI	TTAA	3 cut(s)	72, 224, 276
SatI	GCNGC	1 cut(s)	175
Sau3AI	GATC	5 cut(s)	82, 115, 289, 439, 544
ScrFI	CCNGG	1 cut(s)	418
SduI	GDGCHC	1 cut(s)	609
SetI	ASST	6 cut(s)	52, 137, 195, 470, 519, 556
SfaNI	GCATC	2 cut(s)	337, 559
SfcI	CTRYAG	3 cut(s)	261, 433, 469
SmiMI	CAYNNNNRTG	1 cut(s)	366
Sse9I	AATT	3 cut(s)	100, 324, 626
SsiI	CCGC	2 cut(s)	172, 245
SspMI	CTAG	3 cut(s)	6, 522, 634
StyD4I	CCNGG	1 cut(s)	416
StyI	CCWWGG	1 cut(s)	406
TaaI	ACNGT	1 cut(s)	541
TaiI	ACGT	1 cut(s)	137
TaqI	TCGA	3 cut(s)	106, 345, 454
TasI	AATT	3 cut(s)	100, 324, 626
TfiI	GAWTC	3 cut(s)	10, 108, 257
Tru1I	TTAA	3 cut(s)	72, 224, 276
Tru9I	TTAA	3 cut(s)	72, 224, 276
TscAI	CASTG	1 cut(s)	514
TseFI	GTSAC	1 cut(s)	494
TseI	GCWGC	1 cut(s)	174
Tsp45I	GTSAC	1 cut(s)	494
TspDTI	ATGAA	5 cut(s)	199, 212, 344, 477, 572
TspGWI	ACGGA	1 cut(s)	318
TspRI	CASTG	1 cut(s)	514
XapI	RAATTY	3 cut(s)	100, 324, 626
XbaI	TCTAGA	1 cut(s)	521
XmnI	GAANNNNTTC	2 cut(s)	14, 206
XspI	CTAG	3 cut(s)	6, 522, 634

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.