Belongs to the glycosyl hydrolase 3 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 377, 906 |
| AccB1I | GGYRCC | 1 cut(s) | 107 |
| AccIII | TCCGGA | 1 cut(s) | 42 |
| AciI | CCGC | 2 cut(s) | 155, 207 |
| AcsI | RAATTY | 1 cut(s) | 394 |
| AfaI | GTAC | 1 cut(s) | 1038 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 42, 260, 350, 731, 775 |
| AgeI | ACCGGT | 1 cut(s) | 925 |
| AgsI | TTSAA | 4 cut(s) | 194, 392, 506, 950 |
| AjnI | CCWGG | 4 cut(s) | 103, 254, 446, 859 |
| AleI | CACNNNNGTG | 1 cut(s) | 776 |
| AluBI | AGCT | 6 cut(s) | 239, 571, 715, 750, 922, 955 |
| AluI | AGCT | 6 cut(s) | 239, 571, 715, 750, 922, 955 |
| Alw26I | GTCTC | 1 cut(s) | 824 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 758 |
| Aor13HI | TCCGGA | 1 cut(s) | 42 |
| AoxI | GGCC | 1 cut(s) | 201 |
| ApeKI | GCWGC | 1 cut(s) | 848 |
| ApoI | RAATTY | 1 cut(s) | 394 |
| AsiGI | ACCGGT | 1 cut(s) | 925 |
| AspLEI | GCGC | 2 cut(s) | 28, 103 |
| AspS9I | GGNCC | 2 cut(s) | 201, 734 |
| AsuC2I | CCSGG | 1 cut(s) | 37 |
| AsuHPI | GGTGA | 4 cut(s) | 188, 686, 790, 940 |
| AvaII | GGWCC | 1 cut(s) | 734 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 428, 461, 639, 672 |
| BanI | GGYRCC | 1 cut(s) | 107 |
| BanII | GRGCYC | 1 cut(s) | 255 |
| BbsI | GAAGAC | 2 cut(s) | 294, 802 |
| BbvI | GCAGC | 1 cut(s) | 835 |
| BccI | CCATC | 3 cut(s) | 568, 628, 826 |
| BciT130I | CCWGG | 4 cut(s) | 105, 256, 448, 861 |
| BcnI | CCSGG | 1 cut(s) | 37 |
| BcoDI | GTCTC | 1 cut(s) | 824 |
| BfaI | CTAG | 1 cut(s) | 635 |
| BfmI | CTRYAG | 1 cut(s) | 807 |
| BglII | AGATCT | 1 cut(s) | 524 |
| BisI | GCNGC | 1 cut(s) | 849 |
| BlsI | GCNGC | 1 cut(s) | 850 |
| Bme1390I | CCNGG | 5 cut(s) | 37, 105, 256, 448, 861 |
| Bme18I | GGWCC | 1 cut(s) | 734 |
| BmgT120I | GGNCC | 2 cut(s) | 201, 734 |
| BmiI | GGNNCC | 3 cut(s) | 41, 109, 736 |
| BmrFI | CCNGG | 5 cut(s) | 37, 105, 256, 448, 861 |
| BmsI | GCATC | 5 cut(s) | 58, 142, 257, 448, 826 |
| BpiI | GAAGAC | 2 cut(s) | 294, 802 |
| BpuMI | CCSGG | 1 cut(s) | 37 |
| Bsa29I | ATCGAT | 1 cut(s) | 317 |
| BsaAI | YACGTR | 1 cut(s) | 641 |
| BsaJI | CCNNGG | 2 cut(s) | 254, 349 |
| BsaWI | WCCGGW | 2 cut(s) | 42, 925 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 211, 241 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 42, 260, 350, 731, 775 |
| Bse118I | RCCGGY | 1 cut(s) | 925 |
| BseAI | TCCGGA | 1 cut(s) | 42 |
| BseBI | CCWGG | 4 cut(s) | 105, 256, 448, 861 |
| BseCI | ATCGAT | 1 cut(s) | 317 |
| BseDI | CCNNGG | 2 cut(s) | 254, 349 |
| BseGI | GGATG | 4 cut(s) | 409, 841, 970, 980 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 42, 260, 350, 731, 775 |
| BseMII | CTCAG | 3 cut(s) | 339, 467, 765 |
| BseXI | GCAGC | 1 cut(s) | 835 |
| BshFI | GGCC | 1 cut(s) | 203 |
| BshNI | GGYRCC | 1 cut(s) | 107 |
| BshTI | ACCGGT | 1 cut(s) | 925 |
| BshVI | ATCGAT | 1 cut(s) | 317 |
| BsiSI | CCGG | 3 cut(s) | 37, 43, 926 |
| BslFI | GGGAC | 2 cut(s) | 276, 495 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 42, 260, 350, 731, 775 |
| BsmAI | GTCTC | 1 cut(s) | 824 |
| BsmFI | GGGAC | 2 cut(s) | 276, 495 |
| BsnI | GGCC | 1 cut(s) | 203 |
| Bsp1286I | GDGCHC | 1 cut(s) | 255 |
| Bsp13I | TCCGGA | 1 cut(s) | 42 |
| Bsp143I | GATC | 1 cut(s) | 524 |
| BspACI | CCGC | 2 cut(s) | 155, 207 |
| BspANI | GGCC | 1 cut(s) | 203 |
| BspCNI | CTCAG | 3 cut(s) | 338, 468, 764 |
| BspDI | ATCGAT | 1 cut(s) | 317 |
| BspEI | TCCGGA | 1 cut(s) | 42 |
| BspLI | GGNNCC | 3 cut(s) | 41, 109, 736 |
| BspT107I | GGYRCC | 1 cut(s) | 107 |
| BsrFI | RCCGGY | 1 cut(s) | 925 |
| BssAI | RCCGGY | 1 cut(s) | 925 |
| BssECI | CCNNGG | 2 cut(s) | 254, 349 |
| BssMI | GATC | 1 cut(s) | 524 |
| BssT1I | CCWWGG | 1 cut(s) | 349 |
| Bst2UI | CCWGG | 4 cut(s) | 105, 256, 448, 861 |
| Bst4CI | ACNGT | 2 cut(s) | 128, 808 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 665 |
| BstBAI | YACGTR | 1 cut(s) | 641 |
| BstC8I | GCNNGC | 3 cut(s) | 205, 332, 920 |
| BstDEI | CTNAG | 3 cut(s) | 325, 476, 751 |
| BstF5I | GGATG | 4 cut(s) | 409, 841, 970, 980 |
| BstHHI | GCGC | 2 cut(s) | 28, 103 |
| BstKTI | GATC | 1 cut(s) | 527 |
| BstMAI | GTCTC | 1 cut(s) | 824 |
| BstMBI | GATC | 1 cut(s) | 524 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 107, 161, 340, 665, 845 |
| BstNI | CCWGG | 4 cut(s) | 105, 256, 448, 861 |
| BstNSI | RCATGY | 1 cut(s) | 1020 |
| BstSCI | CCNGG | 5 cut(s) | 35, 103, 254, 446, 859 |
| BstSFI | CTRYAG | 1 cut(s) | 807 |
| BstV1I | GCAGC | 1 cut(s) | 835 |
| BstV2I | GAAGAC | 2 cut(s) | 294, 802 |
| BstX2I | RGATCY | 1 cut(s) | 524 |
| BstYI | RGATCY | 1 cut(s) | 524 |
| Bsu15I | ATCGAT | 1 cut(s) | 317 |
| BsuRI | GGCC | 1 cut(s) | 203 |
| BsuTUI | ATCGAT | 1 cut(s) | 317 |
| BtsCI | GGATG | 4 cut(s) | 409, 841, 970, 980 |
| BtsIMutI | CAGTG | 2 cut(s) | 133, 291 |
| Cac8I | GCNNGC | 3 cut(s) | 205, 332, 920 |
| CaiI | CAGNNNCTG | 1 cut(s) | 758 |
| CfoI | GCGC | 2 cut(s) | 28, 103 |
| Cfr10I | RCCGGY | 1 cut(s) | 925 |
| Cfr13I | GGNCC | 2 cut(s) | 201, 734 |
| ClaI | ATCGAT | 1 cut(s) | 317 |
| Csp6I | GTAC | 1 cut(s) | 1037 |
| CspAI | ACCGGT | 1 cut(s) | 925 |
| CviAII | CATG | 5 cut(s) | 77, 356, 428, 933, 1017 |
| CviQI | GTAC | 1 cut(s) | 1037 |
| DdeI | CTNAG | 3 cut(s) | 325, 476, 751 |
| DpnI | GATC | 1 cut(s) | 526 |
| DpnII | GATC | 1 cut(s) | 524 |
| DraI | TTTAAA | 1 cut(s) | 940 |
| Eco130I | CCWWGG | 1 cut(s) | 349 |
| Eco24I | GRGCYC | 1 cut(s) | 255 |
| Eco32I | GATATC | 1 cut(s) | 789 |
| Eco47I | GGWCC | 1 cut(s) | 734 |
| EcoRII | CCWGG | 4 cut(s) | 103, 254, 446, 859 |
| EcoRV | GATATC | 1 cut(s) | 789 |
| EcoT14I | CCWWGG | 1 cut(s) | 349 |
| EcoT38I | GRGCYC | 1 cut(s) | 255 |
| ErhI | CCWWGG | 1 cut(s) | 349 |
| FaeI | CATG | 5 cut(s) | 80, 359, 431, 936, 1020 |
| FaqI | GGGAC | 2 cut(s) | 276, 495 |
| FatI | CATG | 5 cut(s) | 76, 355, 427, 932, 1016 |
| FauI | CCCGC | 1 cut(s) | 200 |
| FauNDI | CATATG | 1 cut(s) | 685 |
| Fnu4HI | GCNGC | 1 cut(s) | 849 |
| FokI | GGATG | 4 cut(s) | 416, 848, 967, 977 |
| FriOI | GRGCYC | 1 cut(s) | 255 |
| Fsp4HI | GCNGC | 1 cut(s) | 849 |
| FspBI | CTAG | 1 cut(s) | 635 |
| GlaI | GCGC | 2 cut(s) | 27, 102 |
| GluI | GCNGC | 1 cut(s) | 849 |
| HaeIII | GGCC | 1 cut(s) | 203 |
| HapII | CCGG | 3 cut(s) | 37, 43, 926 |
| HhaI | GCGC | 2 cut(s) | 28, 103 |
| Hin1II | CATG | 5 cut(s) | 80, 359, 431, 936, 1020 |
| Hin6I | GCGC | 2 cut(s) | 26, 101 |
| HinP1I | GCGC | 2 cut(s) | 26, 101 |
| HincII | GTYRAC | 1 cut(s) | 7 |
| HindII | GTYRAC | 1 cut(s) | 7 |
| HinfI | GANTC | 7 cut(s) | 46, 90, 211, 415, 721, 765, 781 |
| HpaII | CCGG | 3 cut(s) | 37, 43, 926 |
| HphI | GGTGA | 4 cut(s) | 188, 686, 790, 940 |
| Hpy166II | GTNNAC | 1 cut(s) | 7 |
| Hpy188I | TCNGA | 5 cut(s) | 18, 520, 786, 971, 985 |
| Hpy188III | TCNNGA | 4 cut(s) | 43, 191, 576, 792 |
| Hpy8I | GTNNAC | 1 cut(s) | 7 |
| HpyAV | CCTTC | 3 cut(s) | 573, 671, 863 |
| HpyCH4III | ACNGT | 2 cut(s) | 128, 808 |
| HpyCH4IV | ACGT | 2 cut(s) | 508, 640 |
| HpyCH4V | TGCA | 5 cut(s) | 246, 334, 659, 884, 1020 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 107, 161, 340, 665, 845 |
| HpyF3I | CTNAG | 3 cut(s) | 325, 476, 751 |
| HpySE526I | ACGT | 2 cut(s) | 508, 640 |
| Hsp92II | CATG | 5 cut(s) | 80, 359, 431, 936, 1020 |
| HspAI | GCGC | 2 cut(s) | 26, 101 |
| Kpn2I | TCCGGA | 1 cut(s) | 42 |
| Kzo9I | GATC | 1 cut(s) | 524 |
| LmnI | GCTCC | 4 cut(s) | 45, 232, 712, 960 |
| Lsp1109I | GCAGC | 1 cut(s) | 835 |
| LweI | GCATC | 5 cut(s) | 58, 142, 257, 448, 826 |
| MaeI | CTAG | 1 cut(s) | 635 |
| MaeII | ACGT | 2 cut(s) | 508, 640 |
| MaeIII | GTNAC | 2 cut(s) | 278, 928 |
| MalI | GATC | 1 cut(s) | 526 |
| MboI | GATC | 1 cut(s) | 524 |
| MboII | GAAGA | 3 cut(s) | 299, 396, 807 |
| MflI | RGATCY | 1 cut(s) | 524 |
| MhlI | GDGCHC | 1 cut(s) | 255 |
| MluCI | AATT | 4 cut(s) | 297, 394, 603, 879 |
| MlyI | GAGTC | 1 cut(s) | 99 |
| MmeI | TCCRAC | 3 cut(s) | 567, 949, 1008 |
| MnlI | CCTC | 8 cut(s) | 12, 132, 172, 585, 661, 868, 907, 1008 |
| MroI | TCCGGA | 1 cut(s) | 42 |
| MseI | TTAA | 3 cut(s) | 729, 803, 939 |
| MslI | CAYNNNNRTG | 2 cut(s) | 360, 776 |
| MspA1I | CMGCKG | 1 cut(s) | 207 |
| MspI | CCGG | 3 cut(s) | 37, 43, 926 |
| MspR9I | CCNGG | 5 cut(s) | 37, 105, 256, 448, 861 |
| MvaI | CCWGG | 4 cut(s) | 105, 256, 448, 861 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 107, 161, 340, 665, 845 |
| NciI | CCSGG | 1 cut(s) | 37 |
| NdeI | CATATG | 1 cut(s) | 685 |
| NdeII | GATC | 1 cut(s) | 524 |
| NlaIII | CATG | 5 cut(s) | 80, 359, 431, 936, 1020 |
| NlaIV | GGNNCC | 3 cut(s) | 41, 109, 736 |
| NmuCI | GTSAC | 1 cut(s) | 928 |
| NspI | RCATGY | 1 cut(s) | 1020 |
| OliI | CACNNNNGTG | 1 cut(s) | 776 |
| PfeI | GAWTC | 6 cut(s) | 46, 211, 415, 721, 765, 781 |
| PfoI | TCCNGGA | 1 cut(s) | 446 |
| PinAI | ACCGGT | 1 cut(s) | 925 |
| PkrI | GCNGC | 1 cut(s) | 850 |
| PleI | GAGTC | 1 cut(s) | 98 |
| PpsI | GAGTC | 1 cut(s) | 98 |
| Ppu21I | YACGTR | 1 cut(s) | 641 |
| PsiI | TTATAA | 2 cut(s) | 377, 906 |
| Psp6I | CCWGG | 4 cut(s) | 103, 254, 446, 859 |
| PspGI | CCWGG | 4 cut(s) | 103, 254, 446, 859 |
| PspN4I | GGNNCC | 3 cut(s) | 41, 109, 736 |
| PspPI | GGNCC | 2 cut(s) | 201, 734 |
| PstNI | CAGNNNCTG | 1 cut(s) | 758 |
| PsuI | RGATCY | 1 cut(s) | 524 |
| RsaI | GTAC | 1 cut(s) | 1038 |
| RsaNI | GTAC | 1 cut(s) | 1037 |
| RseI | CAYNNNNRTG | 2 cut(s) | 360, 776 |
| SaqAI | TTAA | 3 cut(s) | 729, 803, 939 |
| SatI | GCNGC | 1 cut(s) | 849 |
| Sau3AI | GATC | 1 cut(s) | 524 |
| Sau96I | GGNCC | 2 cut(s) | 201, 734 |
| SchI | GAGTC | 1 cut(s) | 99 |
| ScrFI | CCNGG | 5 cut(s) | 37, 105, 256, 448, 861 |
| SduI | GDGCHC | 1 cut(s) | 255 |
| SfaNI | GCATC | 5 cut(s) | 58, 142, 257, 448, 826 |
| SfcI | CTRYAG | 1 cut(s) | 807 |
| SinI | GGWCC | 1 cut(s) | 734 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 360, 776 |
| Sse9I | AATT | 4 cut(s) | 297, 394, 603, 879 |
| SsiI | CCGC | 2 cut(s) | 155, 207 |
| SspMI | CTAG | 1 cut(s) | 635 |
| StyD4I | CCNGG | 5 cut(s) | 35, 103, 254, 446, 859 |
| StyI | CCWWGG | 1 cut(s) | 349 |
| TaaI | ACNGT | 2 cut(s) | 128, 808 |
| TaiI | ACGT | 2 cut(s) | 511, 643 |
| TaqI | TCGA | 3 cut(s) | 317, 623, 912 |
| TasI | AATT | 4 cut(s) | 297, 394, 603, 879 |
| TfiI | GAWTC | 6 cut(s) | 46, 211, 415, 721, 765, 781 |
| Tru1I | TTAA | 3 cut(s) | 729, 803, 939 |
| Tru9I | TTAA | 3 cut(s) | 729, 803, 939 |
| TscAI | CASTG | 2 cut(s) | 133, 298 |
| TseFI | GTSAC | 1 cut(s) | 928 |
| TseI | GCWGC | 1 cut(s) | 848 |
| Tsp45I | GTSAC | 1 cut(s) | 928 |
| TspDTI | ATGAA | 3 cut(s) | 65, 372, 672 |
| TspGWI | ACGGA | 1 cut(s) | 1040 |
| TspRI | CASTG | 2 cut(s) | 133, 298 |
| VpaK11BI | GGWCC | 1 cut(s) | 734 |
| XapI | RAATTY | 1 cut(s) | 394 |
| XceI | RCATGY | 1 cut(s) | 1020 |
| XspI | CTAG | 1 cut(s) | 635 |