Rh5BG335900

xylan 1,4-beta-xylosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000625
Physical Location & Seq
Reverse (-)
46829080 .. 46836000
6921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG335900.1

Sequence Viewer

Length: 2031 bp
ATGAAAAGGATAAATTCTTCAGTCCTGTTAGTGGGACTTGCGTGCCTATGTTGCTGGGCGGCCATGACAGAAGCAAGGTTATCTAGACAGCACAGGAAACTAGAAGATAATGAATACATGGCATACAAAGACCCTAGGCAGCCAATCGAGACTCGAATAAATGACCTTATGGATCGAATGACACTAGACGAGAAGGTTGCTCAAATGGCGCAGCTGGACCGTGGTAGTGTCACGGAGGAGCTTATGAAGGAACACAACATAGGAAGTGTACTGAATGGGGGAGAGAGTTTGCCAAGAGTTGGACTTACTGCGTATGAAGACATCGACATGATCAACAAGTTTCAAAATTGGTCACTTCAAAGCCGGCTTGCAATCCCTATTATTTATGGCATTGATGCCGTTCATGGCCACAATAATGTCTACAAGGCCACATTGTTCCCACATAATGTTGGACTTGGAGTTACCAGGGATCCAGATCTTATTAAGAGGATAGGTGCGGCAACTGCCGCTGAAGTTAAAGCTACCGGCATTCAATATGCATTTTCACCATGTGTTGCGGTCTGCAGAGATCCAAGATGGGGTAGGTGTTATGAAAGTTTTAGCGAGGATCCCTCCATTGTAAGACAAATGACTGAAATAATAGCCGGATTGCAAGGCGAAGTCCCAGCTGGACAGAAAGGCCATCCTTACGTTGGTGGAAAGGAGAAGGTAGCTGGCTGTGCAAAGCACTATGTGGGAGATGGTGGCACCATCAAGGGAATCAATGAGAACAACACTGCCATTGATCGGAATGAACTGTTGAAGATCCACATGCCTGGTTACTTGGACTCTATTGACAAGGGTGTGTGCAGTATCATGGTTTCATACTCTAGCTTGAATGGAGTCAAGATGCACGCTAACCATGACCTTGTCACCAACTATCTCAAGGACACCTTGAAGTTCAAGGGTTTTGTCATCTCCGACTGGCAGGGTCTTGACAAAATTACCTATCCGTTCCATTCGAATTACACATACTCGGTTCTTGCTGGTGTTACAGCTGGTATTGACATGTTCATACTTCCCTTCAACTATACAGAGTTCCATGATACTGTACTCGACCTTCACTTTAAAAACCTAATATCCGACAGCCGCATTGATGATGCAGTGTATAGGATACTGAGGATTAAGTTCATAATGGGTCTGTTCGACAACCCCATGGCTGATTATACCTTCGTTGACCGGCTTGGATGCCAGGCTCATCGAGATCTGGCAAGGGAGGCAGTAAGGAAGTCACTTGTTCTTTTGAAGAACGGAAAAAAACCAAACACACCATTCCTGCCTCTCCCTAAAAAGGCCAATAGGATCCTTGTTGCCGGAACTCATGCAAACAATTTGGGATATCAATGTGGTGGTTGGAGTATCACTTGGCAAGGAGTAAGTGGCAATAACAACACTGTTGGAACCACGATTCTAGGTGGCGTCATAGCTACAGTTTACGCGACAACAGAAGTTGTGCACAGTTTAAATCCAGATCCTGATTTTATCAAGGCAAGCAACATTGACTATGCCATTGTTGCTGTTGGTGAACCCCCTTACGCAGAGACGAAAGGGGATAATTTGAATTTGACCATTCCAGAACCAGGCCCAAGCATCATTAGCAATGTCTGCAGCGTAGTCCAGTGTGTTGTTATTGTTGTCTCTGGCCGTCCTCTTGTGATTGAGCCCTATCTTCCAATGATGGATGCCCTTGTGGCTGCATGGCTACCTGGAACTGAAGGCCAAGGAGTTGCTGATGTTCTTTTCGGGGATTATGGATTCACTGGAAAGCTTGCTAGGACTTGGTTCAAGAGAGTAGACCAGCTCCCTATGAATGTTGGTGACAAAAATTATGATCCCCTCTTCCCCTTTGGTTTTGGAATCACAACTGAACCACATATGAGTTATTTAAAAAGTGCATCTGTTGGGAGAAAACTAAGGACCAGAAAGCTAGTGTTGTTGTGCAAGATGGTATTACTCATCACCCTCCAAATATGTGGTTGCTTCCGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

676

Amino Acids

74.7

Weight (kDa)

7.83

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 61 - 388 1.4e-64 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 425 - 634 1.8e-36 Glycosyl hydrolase family 3 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000625)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47010 AT3G47040 AT3G47050
fragaria_vesca FvH4_1g28352 FvH4_3g26960 FvH4_7g05180
malus_domestica MD02G1089300.v1.1 MD02G1259900.v1.1 MD14G1004400.v1.1
prunus_persica Prupe.2G065700_v2.0.a1 Prupe.2G092700_v2.0.a1 Prupe.7G007100_v2.0.a1 Prupe.7G007700_v2.0.a1
pyrus_communis pycom02g22100 pycom07g04670 pycom11g06820 pycom11g15190 pycom14g00200 pycom14g00230
rosa_chinensis RchiOBHm_Chr5g0049431 RchiOBHm_Chr5g0049441 RchiOBHm_Chr5g0079371 RchiOBHm_Chr6g0274311
rosa_laevigata RLG00000006403 RLG00000008403 RLG00000028847 RLG00000034618
rosa_multiflora Rmu_co8385859.1_g000001 Rmu_sc0000376.1_g000001 Rmu_sc0001307.1_g000017 Rmu_sc0001307.1_g000019 Rmu_sc0001900.1_g000031 Rmu_sc0002912.1_g000028 Rmu_sc0006084.1_g000018 Rmu_sc0006888.1_g000063 Rmu_sc0014052.1_g000002 Rmu_sc0014052.1_g000004 Rmu_sc0022931.1_g000004
rosa_roxburghii Rroxscaffold_1G00003570 Rroxscaffold_1G00032240 Rroxscaffold_1G00032250 Rroxscaffold_2G00103090 Rroxscaffold_4G00289090 Rroxscaffold_5G00336970
rosa_rugosa Rorug03G0185900 Rorug04G0020500 Rorug04G0020600 Rorug04G0020600 Rorug04G0020700.1 Rorug04G0020800.1 Rorug04G0020900 Rorug04G0020900 Rorug04G0020900 Rorug05G0248500 Rorug05G0248500 Rorug05G0248500 Rorug05G0248600 Rorug05G0465800
rosa_samantha Rh3CG363400 Rh4DG330500 Rh4DG330600 Rh5AG245600 Rh5AG516800 Rh5AG517600 Rh5AG519000 Rh5AG519500 Rh5BG335900 Rh5BG336000 Rh5BG540700 Rh5BG542700 Rh5DG348200 Rh5DG348300 Rh5DG551900 Rh6BG185500 Rh7DG265300
rosa_wichuraiana Rw1G018240 Rw4G028270 Rw5G030690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 299
AccI GTMKAC 2 cut(s) 420, 1833
AccII CGCG 1 cut(s) 1478
AciI CCGC 6 cut(s) 59, 497, 507, 557, 1129, 2023
AcoI YGGCCR 3 cut(s) 60, 406, 1681
AcsI RAATTY 2 cut(s) 13, 1600
AcuI CTGAAG 2 cut(s) 531, 1773
AcyI GRCGYC 1 cut(s) 1458
AdeI CACNNNGTG 2 cut(s) 551, 733
AfaI GTAC 2 cut(s) 270, 1092
AfiI CCNNNNNNNGG 7 cut(s) 31, 299, 578, 692, 786, 1330, 1619
AflIII ACRYGT 1 cut(s) 1047
AjnI CCWGG 5 cut(s) 464, 814, 1230, 1618, 1744
Alw21I GWGCWC 1 cut(s) 1497
Alw26I GTCTC 3 cut(s) 143, 1574, 1681
Alw44I GTGCAC 1 cut(s) 1493
AlwNI CAGNNNCTG 1 cut(s) 1514
AoxI GGCC 8 cut(s) 60, 406, 426, 679, 1332, 1621, 1681, 1756
ApaLI GTGCAC 1 cut(s) 1493
ApeKI GCWGC 4 cut(s) 139, 211, 1647, 1733
ApoI RAATTY 2 cut(s) 13, 1600
AspA2I CCTAGG 1 cut(s) 134
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 3 cut(s) 217, 1622, 1956
AsuHPI GGTGA 5 cut(s) 537, 904, 1574, 1868, 1990
AsuII TTCGAA 1 cut(s) 1001
AvaII GGWCC 2 cut(s) 217, 1956
AvrII CCTAGG 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 1497
BalI TGGCCA 1 cut(s) 408
BamHI GGATCC 3 cut(s) 469, 607, 1341
BanI GGYRCC 1 cut(s) 746
BanII GRGCYC 1 cut(s) 1704
BarI GAAGNNNNNNTAC 2 cut(s) 1083, 1115
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 1497
BbvI GCAGC 4 cut(s) 151, 223, 1659, 1720
BccI CCATC 6 cut(s) 570, 690, 734, 758, 1711, 1978
BceAI ACGGC 2 cut(s) 383, 1668
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 5 cut(s) 466, 816, 1232, 1620, 1746
BciVI GTATCC 1 cut(s) 1146
BclI TGATCA 1 cut(s) 330
BcoDI GTCTC 3 cut(s) 143, 1574, 1681
BfaI CTAG 8 cut(s) 84, 101, 135, 185, 870, 1451, 1812, 1967
BfmI CTRYAG 3 cut(s) 562, 1467, 1645
BfuI GTATCC 1 cut(s) 1146
BglI GCCNNNNNGGC 1 cut(s) 1730
BglII AGATCT 2 cut(s) 475, 1243
BisI GCNGC 8 cut(s) 60, 140, 212, 498, 507, 1129, 1648, 1734
BlnI CCTAGG 1 cut(s) 134
BlsI GCNGC 8 cut(s) 61, 141, 213, 499, 508, 1130, 1649, 1735
Bme1390I CCNGG 5 cut(s) 466, 816, 1232, 1620, 1746
Bme18I GGWCC 2 cut(s) 217, 1956
BmgT120I GGNCC 3 cut(s) 217, 1622, 1956
BmiI GGNNCC 5 cut(s) 471, 609, 748, 1343, 1441
BmrFI CCNGG 5 cut(s) 466, 816, 1232, 1620, 1746
BmsI GCATC 7 cut(s) 385, 879, 1129, 1217, 1638, 1711, 1943
BpiI GAAGAC 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 596, 628
Bpu14I TTCGAA 1 cut(s) 1001
BpuEI CTTGAG 1 cut(s) 908
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 1458
BsaJI CCNNGG 5 cut(s) 134, 220, 465, 1194, 1759
Bsc4I CCNNNNNNNGG 7 cut(s) 31, 299, 578, 692, 786, 1330, 1619
Bse118I RCCGGY 3 cut(s) 363, 524, 1218
Bse1I ACTGG 3 cut(s) 968, 1657, 1804
Bse3DI GCAATG 1 cut(s) 1645
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 5 cut(s) 466, 816, 1232, 1620, 1746
BseDI CCNNGG 5 cut(s) 134, 220, 465, 1194, 1759
BseGI GGATG 3 cut(s) 682, 1232, 1726
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 7 cut(s) 31, 299, 578, 692, 786, 1330, 1619
BseMI GCAATG 1 cut(s) 1645
BseMII CTCAG 1 cut(s) 1148
BseNI ACTGG 3 cut(s) 968, 1657, 1804
BseRI GAGGAG 1 cut(s) 251
BseSI GKGCMC 1 cut(s) 1497
BseXI GCAGC 4 cut(s) 151, 223, 1659, 1720
BseYI CCCAGC 2 cut(s) 54, 664
BsgI GTGCAG 1 cut(s) 868
Bsh1236I CGCG 1 cut(s) 1478
BshFI GGCC 8 cut(s) 62, 408, 428, 681, 1334, 1623, 1683, 1758
BshNI GGYRCC 1 cut(s) 746
BsiHKAI GWGCWC 1 cut(s) 1497
BsiSI CCGG 5 cut(s) 364, 525, 645, 1219, 1353
BslFI GGGAC 2 cut(s) 48, 647
BslI CCNNNNNNNGG 7 cut(s) 31, 299, 578, 692, 786, 1330, 1619
BsmAI GTCTC 3 cut(s) 143, 1574, 1681
BsmBI CGTCTC 1 cut(s) 1574
BsmFI GGGAC 2 cut(s) 48, 647
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 8 cut(s) 62, 408, 428, 681, 1334, 1623, 1683, 1758
Bsp119I TTCGAA 1 cut(s) 1001
Bsp1286I GDGCHC 2 cut(s) 1497, 1704
Bsp19I CCATGG 1 cut(s) 1194
BspACI CCGC 6 cut(s) 59, 497, 507, 557, 1129, 2023
BspANI GGCC 8 cut(s) 62, 408, 428, 681, 1334, 1623, 1683, 1758
BspCNI CTCAG 1 cut(s) 1149
BspFNI CGCG 1 cut(s) 1478
BspLI GGNNCC 5 cut(s) 471, 609, 748, 1343, 1441
BspMAI CTGCAG 2 cut(s) 566, 1649
BspT104I TTCGAA 1 cut(s) 1001
BspT107I GGYRCC 1 cut(s) 746
BsrDI GCAATG 1 cut(s) 1645
BsrFI RCCGGY 3 cut(s) 363, 524, 1218
BsrI ACTGG 3 cut(s) 968, 1657, 1804
BssAI RCCGGY 3 cut(s) 363, 524, 1218
BssECI CCNNGG 5 cut(s) 134, 220, 465, 1194, 1759
BssNI GRCGYC 1 cut(s) 1458
BssT1I CCWWGG 3 cut(s) 134, 1194, 1759
Bst2UI CCWGG 5 cut(s) 466, 816, 1232, 1620, 1746
Bst4CI ACNGT 6 cut(s) 221, 798, 1090, 1435, 1471, 1499
Bst6I CTCTTC 1 cut(s) 1883
BstACI GRCGYC 1 cut(s) 1458
BstAPI GCANNNNNTGC 1 cut(s) 1644
BstBI TTCGAA 1 cut(s) 1001
BstC8I GCNNGC 7 cut(s) 43, 365, 369, 715, 894, 1531, 1809
BstDEI CTNAG 2 cut(s) 1157, 1952
BstDSI CCRYGG 2 cut(s) 220, 1194
BstF5I GGATG 3 cut(s) 682, 1232, 1726
BstFNI CGCG 1 cut(s) 1478
BstHHI GCGC 1 cut(s) 211
BstMAI GTCTC 3 cut(s) 143, 1574, 1681
BstNI CCWGG 5 cut(s) 466, 816, 1232, 1620, 1746
BstNSI RCATGY 2 cut(s) 814, 1051
BstSCI CCNGG 5 cut(s) 464, 814, 1230, 1618, 1744
BstSFI CTRYAG 3 cut(s) 562, 1467, 1645
BstSLI GKGCMC 1 cut(s) 1497
BstUI CGCG 1 cut(s) 1478
BstV1I GCAGC 4 cut(s) 151, 223, 1659, 1720
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 8 cut(s) 469, 475, 568, 607, 804, 1243, 1341, 1510
BstXI CCANNNNNNTGG 2 cut(s) 815, 2012
BstYI RGATCY 8 cut(s) 469, 475, 568, 607, 804, 1243, 1341, 1510
BsuI GTATCC 1 cut(s) 1146
BsuRI GGCC 8 cut(s) 62, 408, 428, 681, 1334, 1623, 1683, 1758
BtgI CCRYGG 2 cut(s) 220, 1194
BtsCI GGATG 3 cut(s) 682, 1232, 1726
BtsI GCAGTG 2 cut(s) 774, 1149
BtsIMutI CAGTG 5 cut(s) 774, 1149, 1431, 1664, 1797
Cac8I GCNNGC 7 cut(s) 43, 365, 369, 715, 894, 1531, 1809
CaiI CAGNNNCTG 1 cut(s) 1514
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 3 cut(s) 363, 524, 1218
Cfr13I GGNCC 3 cut(s) 217, 1622, 1956
CseI GACGC 1 cut(s) 1447
Csp6I GTAC 2 cut(s) 269, 1091
CviQI GTAC 2 cut(s) 269, 1091
DdeI CTNAG 2 cut(s) 1157, 1952
DraI TTTAAA 3 cut(s) 1108, 1503, 1926
DraIII CACNNNGTG 2 cut(s) 551, 733
EaeI YGGCCR 3 cut(s) 60, 406, 1681
Eam1104I CTCTTC 1 cut(s) 1883
EarI CTCTTC 1 cut(s) 1883
Eco130I CCWWGG 3 cut(s) 134, 1194, 1759
Eco24I GRGCYC 1 cut(s) 1704
Eco32I GATATC 1 cut(s) 1379
Eco47I GGWCC 2 cut(s) 217, 1956
Eco57I CTGAAG 2 cut(s) 531, 1773
EcoRII CCWGG 5 cut(s) 464, 814, 1230, 1618, 1744
EcoRV GATATC 1 cut(s) 1379
EcoT14I CCWWGG 3 cut(s) 134, 1194, 1759
EcoT22I ATGCAT 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 1704
ErhI CCWWGG 3 cut(s) 134, 1194, 1759
Esp3I CGTCTC 1 cut(s) 1574
FalI AAGNNNNNCTT 2 cut(s) 917, 949
FaqI GGGAC 2 cut(s) 48, 647
FauNDI CATATG 1 cut(s) 1914
FbaI TGATCA 1 cut(s) 330
FblI GTMKAC 2 cut(s) 420, 1833
Fnu4HI GCNGC 8 cut(s) 60, 140, 212, 498, 507, 1129, 1648, 1734
FokI GGATG 3 cut(s) 669, 1239, 1733
FriOI GRGCYC 1 cut(s) 1704
Fsp4HI GCNGC 8 cut(s) 60, 140, 212, 498, 507, 1129, 1648, 1734
FspBI CTAG 8 cut(s) 84, 101, 135, 185, 870, 1451, 1812, 1967
GlaI GCGC 1 cut(s) 210
GluI GCNGC 8 cut(s) 60, 140, 212, 498, 507, 1129, 1648, 1734
GsaI CCCAGC 2 cut(s) 58, 668
HaeIII GGCC 8 cut(s) 62, 408, 428, 681, 1334, 1623, 1683, 1758
HapII CCGG 5 cut(s) 364, 525, 645, 1219, 1353
HgaI GACGC 1 cut(s) 1447
HhaI GCGC 1 cut(s) 211
Hin1I GRCGYC 1 cut(s) 1458
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HindIII AAGCTT 1 cut(s) 1805
HinfI GANTC 7 cut(s) 151, 759, 827, 882, 1447, 1794, 1896
HpaII CCGG 5 cut(s) 364, 525, 645, 1219, 1353
HphI GGTGA 5 cut(s) 537, 904, 1574, 1868, 1990
Hpy166II GTNNAC 7 cut(s) 269, 421, 1216, 1474, 1495, 1565, 1834
Hpy188I TCNGA 3 cut(s) 789, 961, 1123
Hpy8I GTNNAC 7 cut(s) 269, 421, 1216, 1474, 1495, 1565, 1834
HpyAV CCTTC 7 cut(s) 187, 241, 700, 1072, 1109, 1219, 1748
HpyCH4III ACNGT 6 cut(s) 221, 798, 1090, 1435, 1471, 1499
HpyCH4IV ACGT 1 cut(s) 690
HpyF3I CTNAG 2 cut(s) 1157, 1952
HpySE526I ACGT 1 cut(s) 690
Hsp92I GRCGYC 1 cut(s) 1458
HspAI GCGC 1 cut(s) 209
KroI GCCGGC 1 cut(s) 363
KroNI GCCGGC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 330
LmnI GCTCC 2 cut(s) 238, 1845
Lsp1109I GCAGC 4 cut(s) 151, 223, 1659, 1720
LweI GCATC 7 cut(s) 385, 879, 1129, 1217, 1638, 1711, 1943
MaeI CTAG 8 cut(s) 84, 101, 135, 185, 870, 1451, 1812, 1967
MaeII ACGT 1 cut(s) 690
MaeIII GTNAC 8 cut(s) 229, 351, 460, 818, 910, 1030, 1269, 1856
MboII GAAGA 7 cut(s) 9, 116, 329, 814, 1297, 1700, 1870
MflI RGATCY 8 cut(s) 469, 475, 568, 607, 804, 1243, 1341, 1510
MhlI GDGCHC 2 cut(s) 1497, 1704
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 8 cut(s) 13, 346, 981, 1003, 1369, 1594, 1600, 1864
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 3 cut(s) 145, 821, 891
MmeI TCCRAC 6 cut(s) 280, 430, 984, 1146, 1373, 1417
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 541
MroNI GCCGGC 1 cut(s) 363
MscI TGGCCA 1 cut(s) 408
MseI TTAA 6 cut(s) 483, 516, 1107, 1164, 1502, 1925
MslI CAYNNNNRTG 2 cut(s) 326, 414
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 4 cut(s) 214, 509, 668, 1037
MspI CCGG 5 cut(s) 364, 525, 645, 1219, 1353
MspR9I CCNGG 5 cut(s) 466, 816, 1232, 1620, 1746
Mva1269I GAATGC 1 cut(s) 528
MvaI CCWGG 5 cut(s) 466, 816, 1232, 1620, 1746
MvnI CGCG 1 cut(s) 1478
NaeI GCCGGC 1 cut(s) 365
NcoI CCATGG 1 cut(s) 1194
NdeI CATATG 1 cut(s) 1914
NgoMIV GCCGGC 1 cut(s) 363
NlaIV GGNNCC 5 cut(s) 471, 609, 748, 1343, 1441
NmuCI GTSAC 5 cut(s) 229, 351, 910, 1269, 1856
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 2 cut(s) 814, 1051
NspV TTCGAA 1 cut(s) 1001
PciI ACATGT 1 cut(s) 1047
PctI GAATGC 1 cut(s) 528
PdiI GCCGGC 1 cut(s) 365
PfeI GAWTC 4 cut(s) 759, 1447, 1794, 1896
PflFI GACNNNGTC 1 cut(s) 908
PflMI CCANNNNNTGG 1 cut(s) 299
PkrI GCNGC 8 cut(s) 61, 141, 213, 499, 508, 1130, 1649, 1735
PleI GAGTC 3 cut(s) 145, 821, 890
PpsI GAGTC 3 cut(s) 145, 821, 890
PscI ACATGT 1 cut(s) 1047
Psp6I CCWGG 5 cut(s) 464, 814, 1230, 1618, 1744
PspFI CCCAGC 2 cut(s) 54, 664
PspGI CCWGG 5 cut(s) 464, 814, 1230, 1618, 1744
PspN4I GGNNCC 5 cut(s) 471, 609, 748, 1343, 1441
PspPI GGNCC 3 cut(s) 217, 1622, 1956
PstI CTGCAG 2 cut(s) 566, 1649
PstNI CAGNNNCTG 1 cut(s) 1514
PsuI RGATCY 8 cut(s) 469, 475, 568, 607, 804, 1243, 1341, 1510
PsyI GACNNNGTC 1 cut(s) 908
PvuII CAGCTG 3 cut(s) 214, 668, 1037
RsaI GTAC 2 cut(s) 270, 1092
RsaNI GTAC 2 cut(s) 269, 1091
RseI CAYNNNNRTG 2 cut(s) 326, 414
SaqAI TTAA 6 cut(s) 483, 516, 1107, 1164, 1502, 1925
SatI GCNGC 8 cut(s) 60, 140, 212, 498, 507, 1129, 1648, 1734
Sau96I GGNCC 3 cut(s) 217, 1622, 1956
SchI GAGTC 3 cut(s) 145, 821, 891
ScrFI CCNGG 5 cut(s) 466, 816, 1232, 1620, 1746
SduI GDGCHC 2 cut(s) 1497, 1704
SfaNI GCATC 7 cut(s) 385, 879, 1129, 1217, 1638, 1711, 1943
SfcI CTRYAG 3 cut(s) 562, 1467, 1645
SfuI TTCGAA 1 cut(s) 1001
SinI GGWCC 2 cut(s) 217, 1956
SmiMI CAYNNNNRTG 2 cut(s) 326, 414
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
Sse9I AATT 8 cut(s) 13, 346, 981, 1003, 1369, 1594, 1600, 1864
SsiI CCGC 6 cut(s) 59, 497, 507, 557, 1129, 2023
SspMI CTAG 8 cut(s) 84, 101, 135, 185, 870, 1451, 1812, 1967
StyD4I CCNGG 5 cut(s) 464, 814, 1230, 1618, 1744
StyI CCWWGG 3 cut(s) 134, 1194, 1759
TaaI ACNGT 6 cut(s) 221, 798, 1090, 1435, 1471, 1499
TaiI ACGT 1 cut(s) 693
TaqI TCGA 8 cut(s) 147, 154, 175, 324, 1001, 1095, 1185, 1240
TasI AATT 8 cut(s) 13, 346, 981, 1003, 1369, 1594, 1600, 1864
TatI WGTACW 2 cut(s) 268, 1090
TauI GCSGC 4 cut(s) 62, 500, 509, 1131
TfiI GAWTC 4 cut(s) 759, 1447, 1794, 1896
Tru1I TTAA 6 cut(s) 483, 516, 1107, 1164, 1502, 1925
Tru9I TTAA 6 cut(s) 483, 516, 1107, 1164, 1502, 1925
TscAI CASTG 5 cut(s) 781, 1149, 1438, 1664, 1804
TseFI GTSAC 5 cut(s) 229, 351, 910, 1269, 1856
TseI GCWGC 4 cut(s) 139, 211, 1647, 1733
Tsp45I GTSAC 5 cut(s) 229, 351, 910, 1269, 1856
TspGWI ACGGA 3 cut(s) 248, 981, 1305
TspRI CASTG 5 cut(s) 781, 1149, 1438, 1664, 1804
Tth111I GACNNNGTC 1 cut(s) 908
Van91I CCANNNNNTGG 1 cut(s) 299
VneI GTGCAC 1 cut(s) 1493
VpaK11BI GGWCC 2 cut(s) 217, 1956
XapI RAATTY 2 cut(s) 13, 1600
XbaI TCTAGA 1 cut(s) 83
XceI RCATGY 2 cut(s) 814, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 689
XmaJI CCTAGG 1 cut(s) 134
XmiI GTMKAC 2 cut(s) 420, 1833
XspI CTAG 8 cut(s) 84, 101, 135, 185, 870, 1451, 1812, 1967
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.