Rorug04G0020900

Lysosomal beta glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000625
Physical Location & Seq
Reverse (-)
3051967 .. 3060733
8767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0020900.1

Sequence Viewer

Length: 2982 bp
ATGCCTATCAGGAAAATGAAGGAGAGCTCAGAGCAACACCTTGTGATCAAAACCCATTTACAGAACACAGCAAACCCAGTTCAGAAGGCACCCAAGACTGGCCAAAATGGCAAAGGCCCACCAACCCCAGAACCTCAAAACCCCAAAACTCAGAACCAAACTTCACCTCCAACTAAGAACAGAGGAAGGAGAAGGGGGAGAGGTGGTCGGAAGTCTGATCAAGGTGATGTTTTTATGCGGCCAAGTTCAAGGCACTGCACGGTGGCTCATATTCCAGCCTCACCAAACTTGGCTGGAGGAGGTGCTTTAGTTAGTAGTACTACAAATGAGTCATTGGAAAATGGTGGAAATTCGTGTGCCATGGAGATGGGTTTTCCCACTTCCAGCAAGTCATTGAGCTTTGCACCTAGGCCTGGCTATGGACAAGCTGGGATAAAGTGTGTTGTAAAGGCCAACCATTTCTTCGCAGAGTTACCAGACAAGGACTTGAACCATTATGATGTTTGCATTGCCCCCGAAGTTACATCGAGAGTTGTGAACAGAGCTATTATGGCCGAACTGGTGAGATTGTACAGAGAATCTGACCTAGGAATGAGACTGCCCGCTTTTGATGGTAGAAAGAGTCTGTATACTGCTGGTGAGCTACCCTTTGTTTGGAAGGAGTTTAACATCAAACTTACTGATGAAGAAGAACGAATCGATGGCCGAAAAAGAGAAAGGGAATATAAAGTGGTGATTAAATTTGTTGCTCGAGCGAACATGTATCATTTGAGCCAGTTTCTAGCCGGTAAATGTGCTGATGCTCCCCAGGAGGCCCTCCAAATTCTTGACATTGTTTTAAGAGAGCTCTCATCAAAAAGGTACTGTCCCATCGGGAGATCCTTCTTTTCACCTAATATCCGAACACCACAACGGCTTGGTGAGGGGTTGGAGTCATGGTGTGGGTTTTACCAAAGTATAAGGCCTACCCAAATGGGTCTTTCCCTCAATATTGATATGGCTTCAGCTGCATTTATTGAGCCTCTTCCTGTAATAGAGTTTGTAGCCCAGCTTCTGGGAAAAGATGTACTATCTAGGACATTGTCTGATGCTGACCGTGTAAAGATTAAGAAGGCTCTTAGAGGGGTGAAAGTTGAAGTTACCCACAGAGGGAGTGTTCGAAGAAAGTATCGAGTATCGGGACTGACATCTCAACCTACAAGAGAGCTTGTGTTTCCTGTTGATGAGAACTCGACGATGAAGTCAGTAATTGAATACTTCCAGGAGATGTATGGATTCACAATTCAACATGGACATCTTCCTTGCCTTCAAGTTGGCGGAAATCAGAAGAAGGCAAATTATTTACCCATGGAGGCCTGCAAAATTGTTGAGGGGCAACGATATACAAAAAGGTTGAATGAAAAACAAATTACTGCTCTCTTAAAAGTTACATGTCAAAGGCCTAGGGATCGTGAAAATGACATCCTCCAGACCGTTCAACAGAATGCTTATGACCAAGACCCTTATGCTAATGAGTTTGGGATCAAAATCAGTGAAAAGCTCGCTTCAGTTGAGGCTCGAATTCTTCCAGCCCCTTGGCTGAAATATCATGAAACAGGAAAAGAAAAGAATTGTTTGCCTCAAGTAGGTCAGTGGAATATGATGAACAAGAAAATGATCAATGGAATGACAGTTAGCCGGTGGGCTTGTATTAACTTCTCACGGAGTGTGCAGGAGAGTGTTGCTCGTGGGTTTTGTAGTGAACTTGGTCAGATGTGTCAAGTCTCTGGCATGGAGTTTAATCCAGAACCTGTAATTCCAATCTACAATGCCAGGCCTGAGCAAGTAGAGAAAGCTTTGAAACATGTATATCATGCATCCATGAACAAAACAAAAGGAAAAGAATTAGAGCTCTTATTGGCTATTTTGCCTGACAACAACGGGTCCCTGTATGGTGATATCAAGCGAATATGTGAAACAGATCTTGGATTAATCTCGCAATGCTGTCTTACAAAGCATGTTTTCAAGATTAGCAAACAGTATTTGGCTAATGTGTCTCTTAAGATAAATGTTAAGATGGGTGGCAGAAACACTGTTCTTTTGGATGCTATTAGCTGCAGGATACCTTTAGTCAGTGATATACCAACCATAATTTTCGGAGCAGACGTGACTCATCCGGAGAATGGAGAAGATACCAGTCCCTCGATTGCTGCGGTAGTGGCTTCCCAGGATTGGCCGGAAGTGACCAAATATGCTGGGTTAGTTTGTGCTCAAGCTCACAGACAGGAACTGATACAAGATTTGTACAAGACATGGCAGGACCCTGTTCGTGGCACAGTCAGTGGCGGTATGATCCGGGATCTTCTGGTTTCCTTTAGGAAAGCAACAGGGCAGAAGCCTCTCAGGATTATATTTTACAGGGATGGCGTAAGTGAAGGGCAATTTTATCAGGTCTTACTTTATGAGCTTGATGCGATTAGGAAGGCGTGTGCTTCTCTGGAACCCAACTATCAACCGCCGGTGACTTTCATTGTAGTACAGAAGCGGCACCACACACGATTATTTGCTAATAACCATAGGGACAGAAGCAGTGTAGACAAGAGTGGCAACATATTGCCTGGCACTGTAGTTGATACCAAGATATGCCATCCTACAGAATTTGATTTCTATCTCTGCAGCCATGCTGGTATTCAGGGGACAAGTCGGCCAGCTCACTACCATGTTCTCTGGGATGAGAATAATTTTACCCCTGATGGAATCCAGTCGTTGACAAACAATCTTTGCTACACATATGCAAGGTGCACGCGCTCTGTGTCTGTTGTACCTCCGGCATATTATGCACATTTGGCTGCTTTTCGTGCCCGATTCTATATGGAGCCAGATCTGCAGGATAATAGCTCTATTGGTCATGCTGCTAAGGGCACACGGGCTGCAGGAGAGTCTGGAGTCCGGCCGTTGCCAGCTTTAAAAGAGAATGTGAAGAGAGTAATGTTTTATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

993

Amino Acids

111.3

Weight (kDa)

9.23

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 146 - 280 1.6e-33 N-terminal domain of argonaute
ArgoL1 PF08699 290 - 340 5e-19 Argonaute linker 1 domain
PAZ PF02170 364 - 473 2.5e-28 PAZ domain
ArgoL2 PF16488 483 - 529 1e-16 Argonaute linker 2 domain
ArgoMid PF16487 540 - 617 1.7e-14 Mid domain of argonaute
Piwi PF02171 632 - 951 1.6e-116 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000625)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47010 AT3G47040 AT3G47050
fragaria_vesca FvH4_1g28352 FvH4_3g26960 FvH4_7g05180
malus_domestica MD02G1089300.v1.1 MD02G1259900.v1.1 MD14G1004400.v1.1
prunus_persica Prupe.2G065700_v2.0.a1 Prupe.2G092700_v2.0.a1 Prupe.7G007100_v2.0.a1 Prupe.7G007700_v2.0.a1
pyrus_communis pycom02g22100 pycom07g04670 pycom11g06820 pycom11g15190 pycom14g00200 pycom14g00230
rosa_chinensis RchiOBHm_Chr5g0049431 RchiOBHm_Chr5g0049441 RchiOBHm_Chr5g0079371 RchiOBHm_Chr6g0274311
rosa_laevigata RLG00000006403 RLG00000008403 RLG00000028847 RLG00000034618
rosa_multiflora Rmu_co8385859.1_g000001 Rmu_sc0000376.1_g000001 Rmu_sc0001307.1_g000017 Rmu_sc0001307.1_g000019 Rmu_sc0001900.1_g000031 Rmu_sc0002912.1_g000028 Rmu_sc0006084.1_g000018 Rmu_sc0006888.1_g000063 Rmu_sc0014052.1_g000002 Rmu_sc0014052.1_g000004 Rmu_sc0022931.1_g000004
rosa_roxburghii Rroxscaffold_1G00003570 Rroxscaffold_1G00032240 Rroxscaffold_1G00032250 Rroxscaffold_2G00103090 Rroxscaffold_4G00289090 Rroxscaffold_5G00336970
rosa_rugosa Rorug03G0185900 Rorug04G0020500 Rorug04G0020600 Rorug04G0020600 Rorug04G0020700.1 Rorug04G0020800.1 Rorug04G0020900 Rorug04G0020900 Rorug04G0020900 Rorug05G0248500 Rorug05G0248500 Rorug05G0248500 Rorug05G0248600 Rorug05G0465800
rosa_samantha Rh3CG363400 Rh4DG330500 Rh4DG330600 Rh5AG245600 Rh5AG516800 Rh5AG517600 Rh5AG519000 Rh5AG519500 Rh5BG335900 Rh5BG336000 Rh5BG540700 Rh5BG542700 Rh5DG348200 Rh5DG348300 Rh5DG551900 Rh6BG185500 Rh7DG265300
rosa_wichuraiana Rw1G018240 Rw4G028270 Rw5G030690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1240
AccB1I GGYRCC 2 cut(s) 88, 2526
AccB7I CCANNNNNTGG 1 cut(s) 1054
AccI GTMKAC 2 cut(s) 629, 2574
AccII CGCG 1 cut(s) 2785
AccIII TCCGGA 1 cut(s) 2155
AciI CCGC 7 cut(s) 238, 603, 1317, 2192, 2325, 2495, 2524
AclWI GGATC 5 cut(s) 873, 1455, 1529, 2326, 2346
AcoI YGGCCR 7 cut(s) 100, 239, 552, 703, 2213, 2684, 2930
AcsI RAATTY 5 cut(s) 349, 740, 822, 1560, 2636
AcuI CTGAAG 2 cut(s) 987, 1530
AdeI CACNNNGTG 2 cut(s) 43, 1706
AfaI GTAC 7 cut(s) 319, 572, 863, 1068, 2285, 2517, 2802
AflII CTTAAG 1 cut(s) 2039
AflIII ACRYGT 3 cut(s) 759, 1430, 1843
AjiI CACGTC 1 cut(s) 2146
AjnI CCWGG 6 cut(s) 412, 807, 1260, 1811, 2205, 2596
AjuI GAANNNNNNNTTGG 4 cut(s) 446, 478, 1947, 1979
Alw21I GWGCWC 5 cut(s) 29, 849, 1893, 2251, 2783
Alw26I GTCTC 3 cut(s) 589, 1768, 2040
Alw44I GTGCAC 1 cut(s) 2779
AlwI GGATC 5 cut(s) 873, 1455, 1529, 2326, 2346
AlwNI CAGNNNCTG 3 cut(s) 1054, 1790, 2269
Ama87I CYCGRG 1 cut(s) 750
Aor13HI TCCGGA 1 cut(s) 2155
ApaLI GTGCAC 1 cut(s) 2779
ApeKI GCWGC 7 cut(s) 1007, 2094, 2189, 2655, 2828, 2891, 2909
ApoI RAATTY 5 cut(s) 349, 740, 822, 1560, 2636
AseI ATTAAT 1 cut(s) 1970
AspA2I CCTAGG 3 cut(s) 407, 586, 1442
AspLEI GCGC 1 cut(s) 2787
AspS9I GGNCC 4 cut(s) 116, 814, 1923, 2299
AsuC2I CCSGG 1 cut(s) 2336
AsuII TTCGAA 1 cut(s) 1159
AvaI CYCGRG 1 cut(s) 750
AvaII GGWCC 2 cut(s) 1923, 2299
AvrII CCTAGG 3 cut(s) 407, 586, 1442
BaeGI GKGCMC 3 cut(s) 2783, 2842, 2903
BalI TGGCCA 1 cut(s) 102
BanI GGYRCC 2 cut(s) 88, 2526
BanII GRGCYC 3 cut(s) 29, 849, 1893
BarI GAAGNNNNNNTAC 2 cut(s) 1035, 1067
BauI CACGAG 1 cut(s) 1725
Bbv12I GWGCWC 5 cut(s) 29, 849, 1893, 2251, 2783
BbvI GCAGC 7 cut(s) 994, 2081, 2176, 2667, 2815, 2878, 2896
BccI CCATC 8 cut(s) 361, 605, 695, 878, 2050, 2396, 2634, 2726
BceAI ACGGC 2 cut(s) 929, 2917
BciT130I CCWGG 6 cut(s) 414, 809, 1262, 1813, 2207, 2598
BciVI GTATCC 1 cut(s) 2094
BclI TGATCA 3 cut(s) 45, 217, 1656
BcnI CCSGG 1 cut(s) 2336
BcoDI GTCTC 3 cut(s) 589, 1768, 2040
BfaI CTAG 5 cut(s) 408, 587, 782, 1074, 1443
BfmI CTRYAG 6 cut(s) 2095, 2604, 2631, 2653, 2864, 2910
BfrI CTTAAG 1 cut(s) 2039
BfuI GTATCC 1 cut(s) 2094
BglI GCCNNNNNGGC 1 cut(s) 108
BglII AGATCT 2 cut(s) 1960, 2860
BisI GCNGC 9 cut(s) 239, 1008, 2095, 2190, 2525, 2656, 2829, 2892, 2910
BlnI CCTAGG 3 cut(s) 407, 586, 1442
BlsI GCNGC 9 cut(s) 240, 1009, 2096, 2191, 2526, 2657, 2830, 2893, 2911
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 7 cut(s) 414, 809, 1262, 1813, 2207, 2336, 2598
Bme18I GGWCC 2 cut(s) 1923, 2299
BmeT110I CYCGRG 1 cut(s) 750
BmgBI CACGTC 1 cut(s) 2146
BmgT120I GGNCC 4 cut(s) 116, 814, 1923, 2299
BmiI GGNNCC 7 cut(s) 90, 1924, 1925, 2301, 2481, 2528, 2856
BmrFI CCNGG 7 cut(s) 414, 809, 1262, 1813, 2207, 2336, 2598
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 5 cut(s) 790, 1078, 1865, 2074, 2440
BmuI ACTGGG 1 cut(s) 71
BplI GAGNNNNNCTC 2 cut(s) 1708, 1740
BpmI CTGGAG 3 cut(s) 315, 1451, 2943
Bpu10I CCTNAGC 2 cut(s) 1818, 2895
Bpu14I TTCGAA 1 cut(s) 1159
BpuEI CTTGAG 2 cut(s) 1605, 2235
BpuMI CCSGG 1 cut(s) 2336
Bsa29I ATCGAT 1 cut(s) 699
BsaBI GATNNNNATC 2 cut(s) 1526, 2646
BsaJI CCNNGG 8 cut(s) 360, 407, 586, 807, 1347, 1442, 1574, 2205
BsaWI WCCGGW 1 cut(s) 2155
BsaXI ACNNNNNCTCC 8 cut(s) 151, 181, 190, 220, 923, 953, 2130, 2160
Bse118I RCCGGY 3 cut(s) 785, 1677, 2497
Bse1I ACTGG 6 cut(s) 77, 103, 564, 775, 2175, 2740
Bse3DI GCAATG 2 cut(s) 507, 1985
Bse8I GATNNNNATC 2 cut(s) 1526, 2646
BseAI TCCGGA 1 cut(s) 2155
BseBI CCWGG 6 cut(s) 414, 809, 1262, 1813, 2207, 2598
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 8 cut(s) 360, 407, 586, 807, 1347, 1442, 1574, 2205
BseGI GGATG 7 cut(s) 1461, 1856, 2089, 2152, 2407, 2626, 2716
BseJI GATNNNNATC 2 cut(s) 1526, 2646
BseMI GCAATG 2 cut(s) 507, 1985
BseMII CTCAG 4 cut(s) 42, 164, 1809, 2395
BseNI ACTGG 6 cut(s) 77, 103, 564, 775, 2175, 2740
BseRI GAGGAG 1 cut(s) 312
BseSI GKGCMC 3 cut(s) 2783, 2842, 2903
BseX3I CGGCCG 1 cut(s) 2930
BseXI GCAGC 7 cut(s) 994, 2081, 2176, 2667, 2815, 2878, 2896
BseYI CCCAGC 3 cut(s) 428, 1047, 2234
BsgI GTGCAG 2 cut(s) 241, 1730
Bsh1236I CGCG 1 cut(s) 2785
Bsh1285I CGRYCG 1 cut(s) 2933
BshNI GGYRCC 2 cut(s) 88, 2526
BshVI ATCGAT 1 cut(s) 699
BsiEI CGRYCG 1 cut(s) 2933
BsiHKAI GWGCWC 5 cut(s) 29, 849, 1893, 2251, 2783
BsiHKCI CYCGRG 1 cut(s) 750
BsiSI CCGG 8 cut(s) 786, 1678, 2156, 2216, 2335, 2498, 2807, 2929
BslFI GGGAC 6 cut(s) 852, 1194, 1909, 2163, 2573, 2689
BsmAI GTCTC 3 cut(s) 589, 1768, 2040
BsmFI GGGAC 6 cut(s) 852, 1194, 1909, 2163, 2573, 2689
BsmI GAATGC 1 cut(s) 1489
BsoBI CYCGRG 1 cut(s) 750
Bsp119I TTCGAA 1 cut(s) 1159
Bsp1286I GDGCHC 7 cut(s) 29, 849, 1893, 2251, 2783, 2842, 2903
Bsp13I TCCGGA 1 cut(s) 2155
Bsp1407I TGTACA 2 cut(s) 570, 2283
Bsp19I CCATGG 2 cut(s) 360, 1347
BspACI CCGC 7 cut(s) 238, 603, 1317, 2192, 2325, 2495, 2524
BspCNI CTCAG 4 cut(s) 41, 163, 1810, 2394
BspDI ATCGAT 1 cut(s) 699
BspEI TCCGGA 1 cut(s) 2155
BspFNI CGCG 1 cut(s) 2785
BspHI TCATGA 1 cut(s) 1588
BspLI GGNNCC 7 cut(s) 90, 1924, 1925, 2301, 2481, 2528, 2856
BspMAI CTGCAG 4 cut(s) 2099, 2657, 2868, 2914
BspPI GGATC 5 cut(s) 873, 1455, 1529, 2326, 2346
BspT104I TTCGAA 1 cut(s) 1159
BspT107I GGYRCC 2 cut(s) 88, 2526
BspTI CTTAAG 1 cut(s) 2039
BsrDI GCAATG 2 cut(s) 507, 1985
BsrFI RCCGGY 3 cut(s) 785, 1677, 2497
BsrGI TGTACA 2 cut(s) 570, 2283
BsrI ACTGG 6 cut(s) 77, 103, 564, 775, 2175, 2740
BssAI RCCGGY 3 cut(s) 785, 1677, 2497
BssECI CCNNGG 8 cut(s) 360, 407, 586, 807, 1347, 1442, 1574, 2205
BssNAI GTATAC 1 cut(s) 630
BssSI CACGAG 1 cut(s) 1725
BssT1I CCWWGG 6 cut(s) 360, 407, 586, 1347, 1442, 1574
Bst1107I GTATAC 1 cut(s) 630
Bst2BI CACGAG 1 cut(s) 1725
Bst2UI CCWGG 6 cut(s) 414, 809, 1262, 1813, 2207, 2598
Bst4CI ACNGT 9 cut(s) 262, 866, 1097, 1474, 1672, 2019, 2074, 2317, 2605
Bst6I CTCTTC 2 cut(s) 1029, 2954
BstAFI CTTAAG 1 cut(s) 2039
BstAPI GCANNNNNTGC 1 cut(s) 2816
BstAUI TGTACA 2 cut(s) 570, 2283
BstBI TTCGAA 1 cut(s) 1159
BstC8I GCNNGC 6 cut(s) 603, 1357, 1542, 2688, 2783, 2940
BstDEI CTNAG 7 cut(s) 28, 150, 174, 1118, 1818, 2381, 2895
BstDSI CCRYGG 2 cut(s) 360, 1347
BstENI CCTNNNNNAGG 1 cut(s) 1623
BstF5I GGATG 7 cut(s) 1461, 1856, 2089, 2152, 2407, 2626, 2716
BstFNI CGCG 1 cut(s) 2785
BstHHI GCGC 1 cut(s) 2787
BstMAI GTCTC 3 cut(s) 589, 1768, 2040
BstMCI CGRYCG 1 cut(s) 2933
BstMWI GCNNNNNNNGC 8 cut(s) 108, 551, 1007, 2198, 2816, 2825, 2837, 2863
BstNI CCWGG 6 cut(s) 414, 809, 1262, 1813, 2207, 2598
BstNSI RCATGY 4 cut(s) 763, 1434, 1847, 2000
BstSCI CCNGG 7 cut(s) 412, 807, 1260, 1811, 2205, 2334, 2596
BstSFI CTRYAG 6 cut(s) 2095, 2604, 2631, 2653, 2864, 2910
BstSLI GKGCMC 3 cut(s) 2783, 2842, 2903
BstUI CGCG 1 cut(s) 2785
BstV1I GCAGC 7 cut(s) 994, 2081, 2176, 2667, 2815, 2878, 2896
BstX2I RGATCY 4 cut(s) 878, 1960, 2338, 2860
BstXI CCANNNNNNTGG 2 cut(s) 367, 1575
BstYI RGATCY 4 cut(s) 878, 1960, 2338, 2860
BstZ17I GTATAC 1 cut(s) 630
BstZI CGGCCG 1 cut(s) 2930
Bsu15I ATCGAT 1 cut(s) 699
BsuI GTATCC 1 cut(s) 2094
BsuTUI ATCGAT 1 cut(s) 699
BtgI CCRYGG 2 cut(s) 360, 1347
BtrI CACGTC 1 cut(s) 2146
BtsCI GGATG 7 cut(s) 1461, 1856, 2089, 2152, 2407, 2626, 2716
BtsI GCAGTG 2 cut(s) 253, 2575
BtsIMutI CAGTG 8 cut(s) 253, 1537, 1637, 2070, 2119, 2326, 2575, 2601
Cac8I GCNNGC 6 cut(s) 603, 1357, 1542, 2688, 2783, 2940
CaiI CAGNNNCTG 3 cut(s) 1054, 1790, 2269
CciI TCATGA 1 cut(s) 1588
CfoI GCGC 1 cut(s) 2787
Cfr10I RCCGGY 3 cut(s) 785, 1677, 2497
Cfr13I GGNCC 4 cut(s) 116, 814, 1923, 2299
ClaI ATCGAT 1 cut(s) 699
Csp6I GTAC 7 cut(s) 318, 571, 862, 1067, 2284, 2516, 2801
CviQI GTAC 7 cut(s) 318, 571, 862, 1067, 2284, 2516, 2801
DdeI CTNAG 7 cut(s) 28, 150, 174, 1118, 1818, 2381, 2895
DraI TTTAAA 1 cut(s) 2946
DraIII CACNNNGTG 2 cut(s) 43, 1706
DrdI GACNNNNNNGTC 1 cut(s) 1240
DseDI GACNNNNNNGTC 1 cut(s) 1240
EaeI YGGCCR 7 cut(s) 100, 239, 552, 703, 2213, 2684, 2930
EagI CGGCCG 1 cut(s) 2930
Eam1104I CTCTTC 2 cut(s) 1029, 2954
EarI CTCTTC 2 cut(s) 1029, 2954
EciI GGCGGA 1 cut(s) 1332
Ecl136II GAGCTC 3 cut(s) 27, 847, 1891
EclXI CGGCCG 1 cut(s) 2930
Eco130I CCWWGG 6 cut(s) 360, 407, 586, 1347, 1442, 1574
Eco147I AGGCCT 5 cut(s) 412, 964, 1355, 1441, 1816
Eco24I GRGCYC 3 cut(s) 29, 849, 1893
Eco32I GATATC 1 cut(s) 1939
Eco47I GGWCC 2 cut(s) 1923, 2299
Eco52I CGGCCG 1 cut(s) 2930
Eco53kI GAGCTC 3 cut(s) 27, 847, 1891
Eco57I CTGAAG 2 cut(s) 987, 1530
Eco88I CYCGRG 1 cut(s) 750
EcoICRI GAGCTC 3 cut(s) 27, 847, 1891
EcoNI CCTNNNNNAGG 1 cut(s) 1623
EcoO109I RGGNCCY 3 cut(s) 814, 1923, 2299
EcoRI GAATTC 1 cut(s) 1560
EcoRII CCWGG 6 cut(s) 412, 807, 1260, 1811, 2205, 2596
EcoRV GATATC 1 cut(s) 1939
EcoT14I CCWWGG 6 cut(s) 360, 407, 586, 1347, 1442, 1574
EcoT22I ATGCAT 1 cut(s) 1858
EcoT38I GRGCYC 3 cut(s) 29, 849, 1893
ErhI CCWWGG 6 cut(s) 360, 407, 586, 1347, 1442, 1574
FaqI GGGAC 6 cut(s) 852, 1194, 1909, 2163, 2573, 2689
FauI CCCGC 1 cut(s) 610
FauNDI CATATG 1 cut(s) 2770
FbaI TGATCA 3 cut(s) 45, 217, 1656
FblI GTMKAC 2 cut(s) 629, 2574
Fnu4HI GCNGC 9 cut(s) 239, 1008, 2095, 2190, 2525, 2656, 2829, 2892, 2910
FokI GGATG 7 cut(s) 1448, 1843, 2096, 2139, 2414, 2613, 2723
FriOI GRGCYC 3 cut(s) 29, 849, 1893
Fsp4HI GCNGC 9 cut(s) 239, 1008, 2095, 2190, 2525, 2656, 2829, 2892, 2910
FspBI CTAG 5 cut(s) 408, 587, 782, 1074, 1443
GlaI GCGC 1 cut(s) 2786
GluI GCNGC 9 cut(s) 239, 1008, 2095, 2190, 2525, 2656, 2829, 2892, 2910
GsaI CCCAGC 3 cut(s) 432, 1051, 2238
GsuI CTGGAG 3 cut(s) 315, 1451, 2943
HapII CCGG 8 cut(s) 786, 1678, 2156, 2216, 2335, 2498, 2807, 2929
HhaI GCGC 1 cut(s) 2787
Hin6I GCGC 1 cut(s) 2785
HinP1I GCGC 1 cut(s) 2785
HincII GTYRAC 1 cut(s) 2748
HindII GTYRAC 1 cut(s) 2748
HindIII AAGCTT 1 cut(s) 1833
HpaII CCGG 8 cut(s) 786, 1678, 2156, 2216, 2335, 2498, 2807, 2929
Hpy166II GTNNAC 6 cut(s) 538, 630, 1742, 2575, 2748, 2781
Hpy8I GTNNAC 6 cut(s) 538, 630, 1742, 2575, 2748, 2781
Hpy99I CGWCG 1 cut(s) 1237
HpyCH4III ACNGT 9 cut(s) 262, 866, 1097, 1474, 1672, 2019, 2074, 2317, 2605
HpyCH4IV ACGT 1 cut(s) 2145
HpyF10VI GCNNNNNNNGC 8 cut(s) 108, 551, 1007, 2198, 2816, 2825, 2837, 2863
HpyF3I CTNAG 7 cut(s) 28, 150, 174, 1118, 1818, 2381, 2895
HpySE526I ACGT 1 cut(s) 2145
HspAI GCGC 1 cut(s) 2785
KflI GGGWCCC 1 cut(s) 1923
Kpn2I TCCGGA 1 cut(s) 2155
Ksp22I TGATCA 3 cut(s) 45, 217, 1656
LmnI GCTCC 3 cut(s) 808, 2138, 2854
Lsp1109I GCAGC 7 cut(s) 994, 2081, 2176, 2667, 2815, 2878, 2896
LweI GCATC 5 cut(s) 790, 1078, 1865, 2074, 2440
MaeI CTAG 5 cut(s) 408, 587, 782, 1074, 1443
MaeII ACGT 1 cut(s) 2145
MaeIII GTNAC 7 cut(s) 471, 520, 1138, 1426, 2146, 2221, 2500
MflI RGATCY 4 cut(s) 878, 1960, 2338, 2860
MhlI GDGCHC 7 cut(s) 29, 849, 1893, 2251, 2783, 2842, 2903
MlsI TGGCCA 1 cut(s) 102
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 6 cut(s) 338, 631, 941, 2143, 2927, 2934
MmeI TCCRAC 3 cut(s) 188, 194, 909
Mox20I TGGCCA 1 cut(s) 102
Mph1103I ATGCAT 1 cut(s) 1858
MroI TCCGGA 1 cut(s) 2155
MscI TGGCCA 1 cut(s) 102
MslI CAYNNNNRTG 3 cut(s) 365, 498, 2697
Msp20I TGGCCA 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 1007
MspCI CTTAAG 1 cut(s) 2039
MspI CCGG 8 cut(s) 786, 1678, 2156, 2216, 2335, 2498, 2807, 2929
MspR9I CCNGG 7 cut(s) 414, 809, 1262, 1813, 2207, 2336, 2598
Mva1269I GAATGC 1 cut(s) 1489
MvaI CCWGG 6 cut(s) 414, 809, 1262, 1813, 2207, 2598
MvnI CGCG 1 cut(s) 2785
MwoI GCNNNNNNNGC 8 cut(s) 108, 551, 1007, 2198, 2816, 2825, 2837, 2863
NciI CCSGG 1 cut(s) 2336
NcoI CCATGG 2 cut(s) 360, 1347
NdeI CATATG 1 cut(s) 2770
NlaIV GGNNCC 7 cut(s) 90, 1924, 1925, 2301, 2481, 2528, 2856
NmuCI GTSAC 3 cut(s) 2146, 2221, 2500
NsiI ATGCAT 1 cut(s) 1858
NspI RCATGY 4 cut(s) 763, 1434, 1847, 2000
NspV TTCGAA 1 cut(s) 1159
PaeR7I CTCGAG 1 cut(s) 750
PagI TCATGA 1 cut(s) 1588
PceI AGGCCT 5 cut(s) 412, 964, 1355, 1441, 1816
PciI ACATGT 3 cut(s) 759, 1430, 1843
PcsI WCGNNNNNNNCGW 1 cut(s) 2142
PctI GAATGC 1 cut(s) 1489
PfeI GAWTC 5 cut(s) 578, 696, 1275, 2736, 2844
PflFI GACNNNGTC 1 cut(s) 1081
PflMI CCANNNNNTGG 1 cut(s) 1054
PfoI TCCNGGA 2 cut(s) 1260, 2334
PkrI GCNGC 9 cut(s) 240, 1009, 2096, 2191, 2526, 2657, 2830, 2893, 2911
PleI GAGTC 6 cut(s) 337, 630, 940, 2143, 2926, 2933
PpsI GAGTC 6 cut(s) 337, 630, 940, 2143, 2926, 2933
PpuMI RGGWCCY 2 cut(s) 1923, 2299
PscI ACATGT 3 cut(s) 759, 1430, 1843
PshBI ATTAAT 1 cut(s) 1970
Psp124BI GAGCTC 3 cut(s) 29, 849, 1893
Psp5II RGGWCCY 2 cut(s) 1923, 2299
Psp6I CCWGG 6 cut(s) 412, 807, 1260, 1811, 2205, 2596
PspFI CCCAGC 3 cut(s) 428, 1047, 2234
PspGI CCWGG 6 cut(s) 412, 807, 1260, 1811, 2205, 2596
PspN4I GGNNCC 7 cut(s) 90, 1924, 1925, 2301, 2481, 2528, 2856
PspPI GGNCC 4 cut(s) 116, 814, 1923, 2299
PspPPI RGGWCCY 2 cut(s) 1923, 2299
PspXI VCTCGAGB 1 cut(s) 750
PstI CTGCAG 4 cut(s) 2099, 2657, 2868, 2914
PstNI CAGNNNCTG 3 cut(s) 1054, 1790, 2269
PsuI RGATCY 4 cut(s) 878, 1960, 2338, 2860
PsyI GACNNNGTC 1 cut(s) 1081
PvuII CAGCTG 1 cut(s) 1007
RsaI GTAC 7 cut(s) 319, 572, 863, 1068, 2285, 2517, 2802
RsaNI GTAC 7 cut(s) 318, 571, 862, 1067, 2284, 2516, 2801
RseI CAYNNNNRTG 3 cut(s) 365, 498, 2697
SacI GAGCTC 3 cut(s) 29, 849, 1893
SatI GCNGC 9 cut(s) 239, 1008, 2095, 2190, 2525, 2656, 2829, 2892, 2910
Sau96I GGNCC 4 cut(s) 116, 814, 1923, 2299
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 6 cut(s) 338, 631, 941, 2143, 2927, 2934
ScrFI CCNGG 7 cut(s) 414, 809, 1262, 1813, 2207, 2336, 2598
SduI GDGCHC 7 cut(s) 29, 849, 1893, 2251, 2783, 2842, 2903
SfaNI GCATC 5 cut(s) 790, 1078, 1865, 2074, 2440
SfcI CTRYAG 6 cut(s) 2095, 2604, 2631, 2653, 2864, 2910
Sfr274I CTCGAG 1 cut(s) 750
SfuI TTCGAA 1 cut(s) 1159
SgrAI CRCCGGYG 1 cut(s) 2497
SinI GGWCC 2 cut(s) 1923, 2299
SlaI CTCGAG 1 cut(s) 750
SmiMI CAYNNNNRTG 3 cut(s) 365, 498, 2697
SmlI CTYRAG 4 cut(s) 750, 1620, 2039, 2250
SmoI CTYRAG 4 cut(s) 750, 1620, 2039, 2250
SseBI AGGCCT 5 cut(s) 412, 964, 1355, 1441, 1816
SsiI CCGC 7 cut(s) 238, 603, 1317, 2192, 2325, 2495, 2524
SspI AATATT 1 cut(s) 991
SspMI CTAG 5 cut(s) 408, 587, 782, 1074, 1443
SstI GAGCTC 3 cut(s) 29, 849, 1893
StuI AGGCCT 5 cut(s) 412, 964, 1355, 1441, 1816
StyD4I CCNGG 7 cut(s) 412, 807, 1260, 1811, 2205, 2334, 2596
StyI CCWWGG 6 cut(s) 360, 407, 586, 1347, 1442, 1574
TaaI ACNGT 9 cut(s) 262, 866, 1097, 1474, 1672, 2019, 2074, 2317, 2605
TaiI ACGT 1 cut(s) 2148
TaqI TCGA 8 cut(s) 527, 699, 751, 1159, 1171, 1232, 1558, 2183
TatI WGTACW 5 cut(s) 317, 570, 1066, 2283, 2515
TauI GCSGC 2 cut(s) 241, 2527
TfiI GAWTC 5 cut(s) 578, 696, 1275, 2736, 2844
TscAI CASTG 8 cut(s) 260, 1537, 1637, 2077, 2119, 2326, 2575, 2608
TseFI GTSAC 3 cut(s) 2146, 2221, 2500
TseI GCWGC 7 cut(s) 1007, 2094, 2189, 2655, 2828, 2891, 2909
Tsp45I GTSAC 3 cut(s) 2146, 2221, 2500
TspDTI ATGAA 8 cut(s) 32, 699, 1253, 1413, 1605, 1658, 1877, 2497
TspGWI ACGGA 1 cut(s) 1717
TspRI CASTG 8 cut(s) 260, 1537, 1637, 2077, 2119, 2326, 2575, 2608
Tth111I GACNNNGTC 1 cut(s) 1081
Van91I CCANNNNNTGG 1 cut(s) 1054
Vha464I CTTAAG 1 cut(s) 2039
VneI GTGCAC 1 cut(s) 2779
VpaK11BI GGWCC 2 cut(s) 1923, 2299
VspI ATTAAT 1 cut(s) 1970
XagI CCTNNNNNAGG 1 cut(s) 1623
XapI RAATTY 5 cut(s) 349, 740, 822, 1560, 2636
XceI RCATGY 4 cut(s) 763, 1434, 1847, 2000
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 1 cut(s) 750
XmaJI CCTAGG 3 cut(s) 407, 586, 1442
XmiI GTMKAC 2 cut(s) 629, 2574
XspI CTAG 5 cut(s) 408, 587, 782, 1074, 1443
ZrmI AGTACT 1 cut(s) 319
Zsp2I ATGCAT 1 cut(s) 1858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.