Rorug04G0020600

Lysosomal beta glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000625
Physical Location & Seq
Forward (+)
3029004 .. 3037480
8477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0020600.1

Sequence Viewer

Length: 3450 bp
ATGCAAGTCCCCAATTCTGAAGAAACCGCCCCCGTAAACCCCCAAACCCCTAAAGACCCCGAAACCTCCGATTCCAATTCGGCAGTGGACGACGATGACAGTTGCGCTGCAGACGACGAGTCGTCGGTTCTCGATGTCTCCGGCAAAAGCTTGGATTTTTCGATTGGGGAGAATTCCGGCGACGGCGTCGGGGCTTTGTATTTGTACAAAAATGTGTATAATTTATTGCCCAAGTCCGTCGGGAGGCTGAAGCGGCTGAGGACGTTGAAGTTTTTCGGGAATGAGATTAATTTGTTCTCTTCGTCGGAGTTTGGGAACTTGGTGGGATTGGAATGCTTGCAGCTCCGGCTATCGTCGCCGGCGTTTGATGGTCTGCCATTGCATAAATTTAAAGGCTTGAAGGAGCTGGAGCTTTCGAAAGTGCCTTCTCGGTCTTCGGCTATACCGATACTGAGTGAGATTGCTAGGCTCAACTGTCTCACCAAGCTCTCTGTTTGTTACTTTTCTATAAGATACCTTCCTCCTGAAATTGGCTGCTTAAGCAAGTTGGAATACCTAGATCTTTCATTCAATAAGATGAAGAGTTTGCCAGCTGAAATTAGTAATTTAAATGCCTTGGTATCGTTAAAAGTTGCCAATAATAAATTATCAGAACTACCTTTGGCTTTGTCCTCGTTGAAAATGTTGGAGAACTTGGACCTGTCGCACAACAGGTTGACATCATTGGGGTCCCTTGATCTTAGCTTGATGCGAAACCTCCAGAATTTAAATCTTCAGCACAACATGCTTCCCATCTACTGTCAAATACCTTCATGGATATGTTGCAATTTGGAAGGTAATGGAAAAGATATGTCCAACGACGATTTTAGCAGTACCTCAGTTGAAATGGATGTATATGAAACCACCATTCAGAAGAATGATGAAAATCGTTTTCACACAGGCTTTCATCATAGCTCACCGAGTATAGTTGGGCCTTCATCTAACAGTAGGTGTTTCACAACTCGGAGATCAGCTGGCCGATGGAAGCGACAGTTCTTGCAGAGGAGAGCTCGTCAAGAACGCTTAAATAACAGCAGGAAGTCGAAAGGTCTGGATCTTCCTAAGCTGCACATGAAGGAGGATGAAGACTGCAAACCAGGCAATATAGATGCTACTTTTGAATCATATCATGAAAGTGCACTGGACATTATAAATTTGGATGATGATAATGATAAAAGTTTACTTTCTGGAGAAGATGAAGGTGTAAACGTCTCCCATGCTGCACATCATGACATGTGTTCGAAGAAGGAGGAGCTTTCTGTAAGAAGTTGCTCATCTCTTAGGGTTAACTCTACTCTAGTAGATAATGGTGATAAAAAGGATTGTTACGAGAGTGATGCATCGTCAACTTGCAATCAAGAGGTTACTGGTGAGCACGATGACGTGGCATATTCAGGAAAATCCAAATGCAGTTCAAAGTCCAAGAGGCCACGAGATGGGGATCTTGATAATAGGAATCTTCAGGATCCCAAACGTTGGAAGTGTGGAGATTGTAGTTCAAGCCTATCTTGCAGAAACATGTCTTGCAAATATAGTAATATGTCATTTTGCAGTGCTGAGGACCATCTTCCAGATGGCTTTTATGATGCAGGACGTGACAGGCCCTTCATGCCACTAGAGAGTTATGAGCAAATTTTCCATCTCGACTCACGTGAAGTTATTCTTGTGGACAGGCTAAGGGATAAAGAGTTGGATGGAATTTTGTGCTCTGCCCGAGATATGGTGTCTCAATTGAAGCAGTTAAATGGTCTAAATACAGATAGAGATCGGGATGATGAGCTACAGATTGCATTATATCTTGCTCTATTTGTATCAGATCATTTTGGAGGGACTGATAGGGCGGCTCTTGTTGAAAGGAGACGAAGAGCTGGTGGTGGTTCAATATCCAGGAAGCCTTTTGTATGCACCTGCTCAATTAGGAACTCTGAATCTATTAGTTTGAACCCCAAACAGTCTCCGGAGACTGTTGAAGATATTGCCTTTTCTGATATCTGTGAGAAATCTCTCCGTTCTATCAAGGCCAGACAAAAATCCGTTGTAGTACCTATAGGAACATTGCAGTTTGGTGTTTGTAGGCATAGAGCATTGCTACTGAAGTACCTATGTGATCGGATGGAACCTCCAATCCCTTGTGAGCTTGTCAGAGGATACCTGGATTTCATGCCACATGCTTGGAATATTATATCAGTCAAGAAAAATGACTCAACTAAGAGAGGTGACTCGATTAAGGGAGGTGACTTGGTTGAGAGAGATGACTCACGGATTCGCATGGTTGTTGATGCATGTCGTCCACATGATGTAAGAGAGGAGACAGATCCTGAATATTATTGCAGGTATATTCCTCTCAGTCGGGCCAGGGTTTCTTTCTCTGGAAGCAGCCCTGCTGATGTTTGTTTGTTTCCTTCATTGTCCTCTTCGGATGAAACTCAAACTGAATCCGGTAGTTCTCTCATTCGGTGTAAATATGGGTCGGCTGACGCAGCAGCAAAGATGCGTACTCTGGAGGTATATGGGACCTCAGTGGATGATATTAGGAACTTTGAATACAGCTGCTTGGGTGAAGTTAGAATTTTAGGTGCTTTGCAACACCCCTGCATAGTAGAGATGTATGGACACAGTATATCATCCAAGTGGGCTCCTTCTATGGATGATAATTCTGGGCACCGTATATTGCAATCTGTAATTTTCATGGAGTATATAAACGGGGGTTCCTTACAGGGTTACATAGAAAAGCTATCTAAAGCAGGTGAGAAGCATGTCCCTGTGGAGTTAGCTTTAAGCATCGCTAAGAATGTTGCATGTGCACTGGTGGAGTTGCACTCAAAACACATTATTCATCGTGACATAAAAAGTGCAAACATTTTGATTGATATCGATAGGAAGACAGCTGATGGAACACCTGTTGTGAAGCTCTGTGATTTTGATAGGGCAGTGCCTCTTAGCTCGTACTTGCATACATGCTGTATTGCCCATGTTGGGATACCTCCTCCTGATGTTTGTGTTGGAACACCTCGCTGGATGGCTCCAGAGGTTCTGCGGGCAATGCATAAACAAAATATTTATGGATTGGAAGTTGATATCTGGTCGTTTGGATGCCTTCTTTTGGAGATGTTGACTTTGCAGATTCCTTACTTGGGATTATCTGAATTAGAGATTCATGACTGTCTATCGATGGGAAAACGACCCAAGTTAACAGATAACTTGGAGGCGCTTCGGCCATTGGAGAAGGCTGCAATGGCTCAATCTGCTGAAGAGCTTGAGCAAACAGAAGCTGACTTAGATGTACTAAGATTTCTGGTTGACTTGTTCTGCCAATGTACTGAGGAGAATCCACAAAGTCGTCCAACGGCTGACAGCCTCTACAAATTGTTGCTCGAACGCAGCACCCAGTTTACAAGTTCAAAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

1149

Amino Acids

128.28

Weight (kDa)

5.66

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 119 - 259 1.7e-12 Leucine-rich repeat region
LRR_8 PF13855 160 - 216 5.9e-06 Leucine rich repeat
EDR1_CTR1_ARMC3_pept PF14381 676 - 743 1.7e-11 EDR1/CTR1/ARMC3, peptidase-like
Pkinase PF00069 862 - 1134 1.1e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 863 - 1135 2.4e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000625)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47010 AT3G47040 AT3G47050
fragaria_vesca FvH4_1g28352 FvH4_3g26960 FvH4_7g05180
malus_domestica MD02G1089300.v1.1 MD02G1259900.v1.1 MD14G1004400.v1.1
prunus_persica Prupe.2G065700_v2.0.a1 Prupe.2G092700_v2.0.a1 Prupe.7G007100_v2.0.a1 Prupe.7G007700_v2.0.a1
pyrus_communis pycom02g22100 pycom07g04670 pycom11g06820 pycom11g15190 pycom14g00200 pycom14g00230
rosa_chinensis RchiOBHm_Chr5g0049431 RchiOBHm_Chr5g0049441 RchiOBHm_Chr5g0079371 RchiOBHm_Chr6g0274311
rosa_laevigata RLG00000006403 RLG00000008403 RLG00000028847 RLG00000034618
rosa_multiflora Rmu_co8385859.1_g000001 Rmu_sc0000376.1_g000001 Rmu_sc0001307.1_g000017 Rmu_sc0001307.1_g000019 Rmu_sc0001900.1_g000031 Rmu_sc0002912.1_g000028 Rmu_sc0006084.1_g000018 Rmu_sc0006888.1_g000063 Rmu_sc0014052.1_g000002 Rmu_sc0014052.1_g000004 Rmu_sc0022931.1_g000004
rosa_roxburghii Rroxscaffold_1G00003570 Rroxscaffold_1G00032240 Rroxscaffold_1G00032250 Rroxscaffold_2G00103090 Rroxscaffold_4G00289090 Rroxscaffold_5G00336970
rosa_rugosa Rorug03G0185900 Rorug04G0020500 Rorug04G0020600 Rorug04G0020600 Rorug04G0020700.1 Rorug04G0020800.1 Rorug04G0020900 Rorug04G0020900 Rorug04G0020900 Rorug05G0248500 Rorug05G0248500 Rorug05G0248500 Rorug05G0248600 Rorug05G0465800
rosa_samantha Rh3CG363400 Rh4DG330500 Rh4DG330600 Rh5AG245600 Rh5AG516800 Rh5AG517600 Rh5AG519000 Rh5AG519500 Rh5BG335900 Rh5BG336000 Rh5BG540700 Rh5BG542700 Rh5DG348200 Rh5DG348300 Rh5DG551900 Rh6BG185500 Rh7DG265300
rosa_wichuraiana Rw1G018240 Rw4G028270 Rw5G030690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1190
AarI CACCTGC 2 cut(s) 1955, 2774
Acc36I ACCTGC 3 cut(s) 1955, 2361, 2774
AccB1I GGYRCC 1 cut(s) 2700
AccB7I CCANNNNNTGG 2 cut(s) 1475, 1515
AccIII TCCGGA 1 cut(s) 1996
AciI CCGC 4 cut(s) 27, 253, 1880, 3076
AclI AACGTT 1 cut(s) 1513
AclWI GGATC 5 cut(s) 1101, 1488, 1499, 1512, 2348
AcoI YGGCCR 2 cut(s) 1015, 3254
AcsI RAATTY 7 cut(s) 172, 386, 763, 1192, 1671, 1737, 2607
AcuI CTGAAG 6 cut(s) 39, 269, 758, 1484, 2153, 3309
AcvI CACGTG 1 cut(s) 1691
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 8 cut(s) 206, 874, 2082, 2138, 2536, 2987, 3324, 3358
AfiI CCNNNNNNNGG 8 cut(s) 243, 530, 1475, 1515, 1759, 3015, 3142, 3173
AflII CTTAAG 1 cut(s) 538
AflIII ACRYGT 2 cut(s) 1272, 1557
AhdI GACNNNNNGTC 2 cut(s) 118, 121
AjiI CACGTC 2 cut(s) 1423, 1634
AjnI CCWGG 4 cut(s) 1135, 1925, 2190, 2393
AloI GAACNNNNNNTCC 2 cut(s) 2148, 2180
Alw21I GWGCWC 5 cut(s) 1051, 1180, 1416, 1748, 2845
Alw26I GTCTC 8 cut(s) 142, 482, 1255, 1770, 1891, 1994, 1998, 2342
Alw44I GTGCAC 2 cut(s) 1176, 2841
AlwI GGATC 5 cut(s) 1101, 1488, 1499, 1512, 2348
AlwNI CAGNNNCTG 2 cut(s) 2357, 3311
Ama87I CYCGRG 1 cut(s) 1752
Aor13HI TCCGGA 1 cut(s) 1996
AoxI GGCC 7 cut(s) 971, 1015, 1466, 1640, 2058, 2391, 3254
ApaLI GTGCAC 2 cut(s) 1176, 2841
ApoI RAATTY 7 cut(s) 172, 386, 763, 1192, 1671, 1737, 2607
AseI ATTAAT 1 cut(s) 288
Asp700I GAANNNNTTC 2 cut(s) 272, 1698
AspLEI GCGC 2 cut(s) 107, 3250
AspS9I GGNCC 7 cut(s) 697, 729, 971, 1600, 1641, 2391, 2553
AsuHPI GGTGA 8 cut(s) 472, 948, 1361, 1421, 2267, 2285, 2609, 2798
AsuII TTCGAA 2 cut(s) 416, 1280
AvaI CYCGRG 1 cut(s) 1752
AvaII GGWCC 4 cut(s) 697, 729, 1600, 2553
BaeGI GKGCMC 3 cut(s) 1180, 2703, 2845
BaeI ACNNNNGTAYC 2 cut(s) 2120, 2153
BamHI GGATCC 1 cut(s) 1504
BanI GGYRCC 1 cut(s) 2700
BanII GRGCYC 2 cut(s) 1051, 2677
BauI CACGAG 1 cut(s) 1470
BbrPI CACGTG 1 cut(s) 1691
BbsI GAAGAC 3 cut(s) 426, 1131, 2927
Bbv12I GWGCWC 5 cut(s) 1051, 1180, 1416, 1748, 2845
BbvCI CCTCAGC 2 cut(s) 257, 1596
BceAI ACGGC 2 cut(s) 199, 3402
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 4 cut(s) 1137, 1927, 2192, 2395
BciVI GTATCC 2 cut(s) 2180, 3012
BcoDI GTCTC 8 cut(s) 142, 482, 1255, 1770, 1891, 1994, 1998, 2342
BfaI CTAG 4 cut(s) 465, 557, 1337, 1655
BfmI CTRYAG 3 cut(s) 108, 1820, 2085
BfoI RGCGCY 1 cut(s) 3251
BfrI CTTAAG 1 cut(s) 538
BfuAI ACCTGC 3 cut(s) 1955, 2361, 2774
BfuI GTATCC 2 cut(s) 2180, 3012
BglII AGATCT 1 cut(s) 559
Bme1390I CCNGG 4 cut(s) 1137, 1927, 2192, 2395
Bme18I GGWCC 4 cut(s) 697, 729, 1600, 2553
BmeRI GACNNNNNGTC 2 cut(s) 118, 121
BmeT110I CYCGRG 1 cut(s) 1752
BmgBI CACGTC 2 cut(s) 1423, 1634
BmgT120I GGNCC 7 cut(s) 697, 729, 971, 1600, 1641, 2391, 2553
BmiI GGNNCC 9 cut(s) 730, 731, 1506, 2157, 2554, 2676, 2702, 2749, 3063
BmrFI CCNGG 4 cut(s) 1137, 1927, 2192, 2395
BmrI ACTGGG 1 cut(s) 3421
BmsI GCATC 9 cut(s) 738, 1138, 1366, 1388, 1615, 2308, 2520, 2829, 3122
BmuI ACTGGG 1 cut(s) 3421
BpiI GAAGAC 3 cut(s) 426, 1131, 2927
BplI GAGNNNNNCTC 4 cut(s) 1033, 1065, 2843, 2875
BpmI CTGGAG 5 cut(s) 428, 743, 1248, 2561, 3048
Bpu10I CCTNAGC 4 cut(s) 257, 1101, 1596, 1715
Bpu14I TTCGAA 2 cut(s) 416, 1280
BpuEI CTTGAG 1 cut(s) 3317
Bsa29I ATCGAT 2 cut(s) 2913, 3209
BsaAI YACGTR 1 cut(s) 1691
BsaBI GATNNNNATC 4 cut(s) 924, 1479, 1803, 2909
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 615, 2394
BsaWI WCCGGW 2 cut(s) 1996, 2477
BsaXI ACNNNNNCTCC 2 cut(s) 1036, 1066
Bsc4I CCNNNNNNNGG 8 cut(s) 243, 530, 1475, 1515, 1759, 3015, 3142, 3173
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 4 cut(s) 1185, 1411, 2850, 3427
Bse3DI GCAATG 5 cut(s) 377, 2093, 2123, 3087, 3279
Bse8I GATNNNNATC 4 cut(s) 924, 1479, 1803, 2909
BseAI TCCGGA 1 cut(s) 1996
BseBI CCWGG 4 cut(s) 1137, 1927, 2192, 2395
BseCI ATCGAT 2 cut(s) 2913, 3209
BseDI CCNNGG 2 cut(s) 615, 2394
BseJI GATNNNNATC 4 cut(s) 924, 1479, 1803, 2909
BseLI CCNNNNNNNGG 8 cut(s) 243, 530, 1475, 1515, 1759, 3015, 3142, 3173
BseMI GCAATG 5 cut(s) 377, 2093, 2123, 3087, 3279
BseMII CTCAG 7 cut(s) 248, 443, 891, 1587, 2398, 2571, 3351
BseNI ACTGG 4 cut(s) 1185, 1411, 2850, 3427
BseRI GAGGAG 5 cut(s) 1057, 1304, 2360, 3015, 3377
BseSI GKGCMC 3 cut(s) 1180, 2703, 2845
BsgI GTGCAG 2 cut(s) 1091, 1245
BshFI GGCC 7 cut(s) 973, 1017, 1468, 1642, 2060, 2393, 3256
BshNI GGYRCC 1 cut(s) 2700
BshVI ATCGAT 2 cut(s) 2913, 3209
BsiHKAI GWGCWC 5 cut(s) 1051, 1180, 1416, 1748, 2845
BsiHKCI CYCGRG 1 cut(s) 1752
BsiSI CCGG 6 cut(s) 141, 177, 346, 359, 1997, 2478
BslFI GGGAC 4 cut(s) 715, 1882, 2566, 2783
BslI CCNNNNNNNGG 8 cut(s) 243, 530, 1475, 1515, 1759, 3015, 3142, 3173
BsmAI GTCTC 8 cut(s) 142, 482, 1255, 1770, 1891, 1994, 1998, 2342
BsmBI CGTCTC 2 cut(s) 1255, 1891
BsmFI GGGAC 4 cut(s) 715, 1882, 2566, 2783
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 7 cut(s) 973, 1017, 1468, 1642, 2060, 2393, 3256
BsoBI CYCGRG 1 cut(s) 1752
Bsp119I TTCGAA 2 cut(s) 416, 1280
Bsp1286I GDGCHC 7 cut(s) 1051, 1180, 1416, 1748, 2677, 2703, 2845
Bsp13I TCCGGA 1 cut(s) 1996
Bsp1407I TGTACA 1 cut(s) 204
BspACI CCGC 4 cut(s) 27, 253, 1880, 3076
BspANI GGCC 7 cut(s) 973, 1017, 1468, 1642, 2060, 2393, 3256
BspCNI CTCAG 7 cut(s) 249, 444, 890, 1588, 2397, 2570, 3352
BspDI ATCGAT 2 cut(s) 2913, 3209
BspEI TCCGGA 1 cut(s) 1996
BspHI TCATGA 3 cut(s) 1168, 1267, 3196
BspLI GGNNCC 9 cut(s) 730, 731, 1506, 2157, 2554, 2676, 2702, 2749, 3063
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 3 cut(s) 1955, 2361, 2774
BspPI GGATC 5 cut(s) 1101, 1488, 1499, 1512, 2348
BspQI GCTCTTC 2 cut(s) 1897, 3285
BspT104I TTCGAA 2 cut(s) 416, 1280
BspT107I GGYRCC 1 cut(s) 2700
BspTI CTTAAG 1 cut(s) 538
BsrDI GCAATG 5 cut(s) 377, 2093, 2123, 3087, 3279
BsrFI RCCGGY 1 cut(s) 358
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 4 cut(s) 1185, 1411, 2850, 3427
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 2 cut(s) 615, 2394
BssNI GRCGYC 1 cut(s) 186
BssSI CACGAG 1 cut(s) 1470
BssT1I CCWWGG 1 cut(s) 615
Bst2BI CACGAG 1 cut(s) 1470
Bst2UI CCWGG 4 cut(s) 1137, 1927, 2192, 2395
Bst6I CTCTTC 5 cut(s) 304, 575, 1897, 2458, 3285
BstACI GRCGYC 1 cut(s) 186
BstAFI CTTAAG 1 cut(s) 538
BstAPI GCANNNNNTGC 1 cut(s) 784
BstAUI TGTACA 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 1691
BstBI TTCGAA 2 cut(s) 416, 1280
BstC8I GCNNGC 5 cut(s) 338, 360, 591, 1015, 3078
BstH2I RGCGCY 1 cut(s) 3251
BstHHI GCGC 2 cut(s) 107, 3250
BstMAI GTCTC 8 cut(s) 142, 482, 1255, 1770, 1891, 1994, 1998, 2342
BstNI CCWGG 4 cut(s) 1137, 1927, 2192, 2395
BstNSI RCATGY 8 cut(s) 787, 1276, 1561, 2210, 2325, 2798, 2841, 3000
BstSCI CCNGG 4 cut(s) 1135, 1925, 2190, 2393
BstSFI CTRYAG 3 cut(s) 108, 1820, 2085
BstSLI GKGCMC 3 cut(s) 1180, 2703, 2845
BstV2I GAAGAC 3 cut(s) 426, 1131, 2927
BstX2I RGATCY 5 cut(s) 559, 1093, 1480, 1504, 2353
BstXI CCANNNNNNTGG 1 cut(s) 2211
BstYI RGATCY 5 cut(s) 559, 1093, 1480, 1504, 2353
Bsu15I ATCGAT 2 cut(s) 2913, 3209
BsuI GTATCC 2 cut(s) 2180, 3012
BsuRI GGCC 7 cut(s) 973, 1017, 1468, 1642, 2060, 2393, 3256
BsuTUI ATCGAT 2 cut(s) 2913, 3209
BtgZI GCGATG 1 cut(s) 2806
BtrI CACGTC 2 cut(s) 1423, 1634
BtsI GCAGTG 3 cut(s) 90, 1597, 2976
BtsIMutI CAGTG 6 cut(s) 90, 1178, 1597, 2565, 2843, 2976
BveI ACCTGC 3 cut(s) 1955, 2361, 2774
Cac8I GCNNGC 5 cut(s) 338, 360, 591, 1015, 3078
CaiI CAGNNNCTG 2 cut(s) 2357, 3311
CciI TCATGA 3 cut(s) 1168, 1267, 3196
CfoI GCGC 2 cut(s) 107, 3250
Cfr10I RCCGGY 1 cut(s) 358
Cfr13I GGNCC 7 cut(s) 697, 729, 971, 1600, 1641, 2391, 2553
ClaI ATCGAT 2 cut(s) 2913, 3209
CseI GACGC 2 cut(s) 175, 2525
Csp6I GTAC 8 cut(s) 205, 873, 2081, 2137, 2535, 2986, 3323, 3357
CviQI GTAC 8 cut(s) 205, 873, 2081, 2137, 2535, 2986, 3323, 3357
DraI TTTAAA 3 cut(s) 391, 609, 768
DriI GACNNNNNGTC 2 cut(s) 118, 121
EaeI YGGCCR 2 cut(s) 1015, 3254
Eam1104I CTCTTC 5 cut(s) 304, 575, 1897, 2458, 3285
Eam1105I GACNNNNNGTC 2 cut(s) 118, 121
EarI CTCTTC 5 cut(s) 304, 575, 1897, 2458, 3285
Ecl136II GAGCTC 1 cut(s) 1049
Eco130I CCWWGG 1 cut(s) 615
Eco24I GRGCYC 2 cut(s) 1051, 2677
Eco32I GATATC 3 cut(s) 2029, 2911, 3118
Eco47I GGWCC 4 cut(s) 697, 729, 1600, 2553
Eco53kI GAGCTC 1 cut(s) 1049
Eco57I CTGAAG 6 cut(s) 39, 269, 758, 1484, 2153, 3309
Eco72I CACGTG 1 cut(s) 1691
Eco88I CYCGRG 1 cut(s) 1752
EcoICRI GAGCTC 1 cut(s) 1049
EcoO109I RGGNCCY 3 cut(s) 729, 1641, 2553
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 4 cut(s) 1135, 1925, 2190, 2393
EcoRV GATATC 3 cut(s) 2029, 2911, 3118
EcoT14I CCWWGG 1 cut(s) 615
EcoT22I ATGCAT 3 cut(s) 1381, 2323, 3087
EcoT38I GRGCYC 2 cut(s) 1051, 2677
ErhI CCWWGG 1 cut(s) 615
Esp3I CGTCTC 2 cut(s) 1255, 1891
FalI AAGNNNNNCTT 4 cut(s) 1531, 1563, 1686, 1718
FaqI GGGAC 4 cut(s) 715, 1882, 2566, 2783
FauI CCCGC 1 cut(s) 3069
FriOI GRGCYC 2 cut(s) 1051, 2677
FspBI CTAG 4 cut(s) 465, 557, 1337, 1655
GlaI GCGC 2 cut(s) 106, 3249
GsuI CTGGAG 5 cut(s) 428, 743, 1248, 2561, 3048
HaeII RGCGCY 1 cut(s) 3251
HaeIII GGCC 7 cut(s) 973, 1017, 1468, 1642, 2060, 2393, 3256
HapII CCGG 6 cut(s) 141, 177, 346, 359, 1997, 2478
HgaI GACGC 2 cut(s) 175, 2525
HhaI GCGC 2 cut(s) 107, 3250
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 2 cut(s) 105, 3248
HinP1I GCGC 2 cut(s) 105, 3248
HincII GTYRAC 6 cut(s) 717, 1327, 1386, 3153, 3231, 3340
HindII GTYRAC 6 cut(s) 717, 1327, 1386, 3153, 3231, 3340
HindIII AAGCTT 1 cut(s) 148
HpaI GTTAAC 2 cut(s) 1327, 3231
HpaII CCGG 6 cut(s) 141, 177, 346, 359, 1997, 2478
HphI GGTGA 8 cut(s) 472, 948, 1361, 1421, 2267, 2285, 2609, 2798
Hpy99I CGWCG 9 cut(s) 95, 119, 127, 185, 191, 242, 307, 358, 863
HpyCH4IV ACGT 6 cut(s) 263, 1248, 1422, 1513, 1633, 1690
HpySE526I ACGT 6 cut(s) 263, 1248, 1422, 1513, 1633, 1690
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 2 cut(s) 105, 3248
KflI GGGWCCC 1 cut(s) 729
Kpn2I TCCGGA 1 cut(s) 1996
KroI GCCGGC 1 cut(s) 358
KroNI GCCGGC 1 cut(s) 360
KspAI GTTAAC 2 cut(s) 1327, 3231
LguI GCTCTTC 2 cut(s) 1897, 3285
LmnI GCTCC 6 cut(s) 348, 403, 409, 1291, 2680, 3067
LweI GCATC 9 cut(s) 738, 1138, 1366, 1388, 1615, 2308, 2520, 2829, 3122
MaeI CTAG 4 cut(s) 465, 557, 1337, 1655
MaeII ACGT 6 cut(s) 263, 1248, 1422, 1513, 1633, 1690
MaeIII GTNAC 8 cut(s) 497, 1364, 1402, 1634, 2255, 2273, 2759, 2879
MfeI CAATTG 1 cut(s) 1769
MflI RGATCY 5 cut(s) 559, 1093, 1480, 1504, 2353
MhlI GDGCHC 7 cut(s) 1051, 1180, 1416, 1748, 2677, 2703, 2845
MlyI GAGTC 5 cut(s) 128, 1679, 2234, 2252, 2288
MmeI TCCRAC 8 cut(s) 285, 528, 666, 879, 1496, 1710, 3022, 3407
Mph1103I ATGCAT 3 cut(s) 1381, 2323, 3087
MreI CGCCGGCG 1 cut(s) 358
MroI TCCGGA 1 cut(s) 1996
MroNI GCCGGC 1 cut(s) 358
MroXI GAANNNNTTC 2 cut(s) 272, 1698
MslI CAYNNNNRTG 3 cut(s) 817, 1173, 2668
MspA1I CMGCKG 4 cut(s) 593, 1013, 2589, 2927
MspCI CTTAAG 1 cut(s) 538
MspI CCGG 6 cut(s) 141, 177, 346, 359, 1997, 2478
MspR9I CCNGG 4 cut(s) 1137, 1927, 2192, 2395
MunI CAATTG 1 cut(s) 1769
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 4 cut(s) 1137, 1927, 2192, 2395
NaeI GCCGGC 1 cut(s) 360
NgoMIV GCCGGC 1 cut(s) 358
NlaIV GGNNCC 9 cut(s) 730, 731, 1506, 2157, 2554, 2676, 2702, 2749, 3063
NmuCI GTSAC 4 cut(s) 1634, 2255, 2273, 2879
NsiI ATGCAT 3 cut(s) 1381, 2323, 3087
NspI RCATGY 8 cut(s) 787, 1276, 1561, 2210, 2325, 2798, 2841, 3000
NspV TTCGAA 2 cut(s) 416, 1280
PagI TCATGA 3 cut(s) 1168, 1267, 3196
PaqCI CACCTGC 2 cut(s) 1955, 2774
PciI ACATGT 2 cut(s) 1272, 1557
PciSI GCTCTTC 2 cut(s) 1897, 3285
PcsI WCGNNNNNNNCGW 2 cut(s) 359, 443
PctI GAATGC 1 cut(s) 338
PdiI GCCGGC 1 cut(s) 360
PdmI GAANNNNTTC 2 cut(s) 272, 1698
PfeI GAWTC 9 cut(s) 71, 1160, 1495, 1967, 2302, 2474, 3163, 3193, 3367
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 2 cut(s) 1475, 1515
PfoI TCCNGGA 1 cut(s) 1925
PleI GAGTC 5 cut(s) 127, 1679, 2234, 2252, 2288
PmaCI CACGTG 1 cut(s) 1691
PmlI CACGTG 1 cut(s) 1691
PpsI GAGTC 5 cut(s) 127, 1679, 2234, 2252, 2288
Ppu21I YACGTR 1 cut(s) 1691
PpuMI RGGWCCY 2 cut(s) 729, 2553
PscI ACATGT 2 cut(s) 1272, 1557
PshBI ATTAAT 1 cut(s) 288
PsiI TTATAA 1 cut(s) 1190
Psp124BI GAGCTC 1 cut(s) 1051
Psp1406I AACGTT 1 cut(s) 1513
Psp5II RGGWCCY 2 cut(s) 729, 2553
Psp6I CCWGG 4 cut(s) 1135, 1925, 2190, 2393
PspCI CACGTG 1 cut(s) 1691
PspGI CCWGG 4 cut(s) 1135, 1925, 2190, 2393
PspN4I GGNNCC 9 cut(s) 730, 731, 1506, 2157, 2554, 2676, 2702, 2749, 3063
PspPI GGNCC 7 cut(s) 697, 729, 971, 1600, 1641, 2391, 2553
PspPPI RGGWCCY 2 cut(s) 729, 2553
PsrI GAACNNNNNNTAC 2 cut(s) 2567, 2599
PstI CTGCAG 1 cut(s) 112
PstNI CAGNNNCTG 2 cut(s) 2357, 3311
PsuI RGATCY 5 cut(s) 559, 1093, 1480, 1504, 2353
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 4 cut(s) 593, 1013, 2589, 2927
RsaI GTAC 8 cut(s) 206, 874, 2082, 2138, 2536, 2987, 3324, 3358
RsaNI GTAC 8 cut(s) 205, 873, 2081, 2137, 2535, 2986, 3323, 3357
RseI CAYNNNNRTG 3 cut(s) 817, 1173, 2668
SacI GAGCTC 1 cut(s) 1051
SapI GCTCTTC 2 cut(s) 1897, 3285
Sau96I GGNCC 7 cut(s) 697, 729, 971, 1600, 1641, 2391, 2553
SchI GAGTC 5 cut(s) 128, 1679, 2234, 2252, 2288
ScrFI CCNGG 4 cut(s) 1137, 1927, 2192, 2395
SduI GDGCHC 7 cut(s) 1051, 1180, 1416, 1748, 2677, 2703, 2845
SfaNI GCATC 9 cut(s) 738, 1138, 1366, 1388, 1615, 2308, 2520, 2829, 3122
SfcI CTRYAG 3 cut(s) 108, 1820, 2085
SfuI TTCGAA 2 cut(s) 416, 1280
SgrAI CRCCGGYG 1 cut(s) 358
SinI GGWCC 4 cut(s) 697, 729, 1600, 2553
SmiI ATTTAAAT 2 cut(s) 609, 768
SmiMI CAYNNNNRTG 3 cut(s) 817, 1173, 2668
SmlI CTYRAG 2 cut(s) 538, 3296
SmoI CTYRAG 2 cut(s) 538, 3296
SsiI CCGC 4 cut(s) 27, 253, 1880, 3076
SspI AATATT 3 cut(s) 2218, 2363, 3097
SspMI CTAG 4 cut(s) 465, 557, 1337, 1655
SstI GAGCTC 1 cut(s) 1051
StyD4I CCNGG 4 cut(s) 1135, 1925, 2190, 2393
StyI CCWWGG 1 cut(s) 615
SwaI ATTTAAAT 2 cut(s) 609, 768
TaiI ACGT 6 cut(s) 266, 1251, 1425, 1516, 1636, 1693
TaqII GACCGA 1 cut(s) 420
TatI WGTACW 3 cut(s) 204, 3322, 3356
TauI GCSGC 2 cut(s) 256, 1883
TfiI GAWTC 9 cut(s) 71, 1160, 1495, 1967, 2302, 2474, 3163, 3193, 3367
TscAI CASTG 6 cut(s) 90, 1185, 1597, 2565, 2850, 2976
TseFI GTSAC 4 cut(s) 1634, 2255, 2273, 2879
Tsp45I GTSAC 4 cut(s) 1634, 2255, 2273, 2879
TspGWI ACGGA 4 cut(s) 226, 2036, 2062, 2314
TspRI CASTG 6 cut(s) 90, 1185, 1597, 2565, 2850, 2976
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 2 cut(s) 1475, 1515
Vha464I CTTAAG 1 cut(s) 538
VneI GTGCAC 2 cut(s) 1176, 2841
VpaK11BI GGWCC 4 cut(s) 697, 729, 1600, 2553
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 7 cut(s) 172, 386, 763, 1192, 1671, 1737, 2607
XceI RCATGY 8 cut(s) 787, 1276, 1561, 2210, 2325, 2798, 2841, 3000
XcmI CCANNNNNNNNNTGG 2 cut(s) 82, 1610
XmnI GAANNNNTTC 2 cut(s) 272, 1698
XspI CTAG 4 cut(s) 465, 557, 1337, 1655
Zsp2I ATGCAT 3 cut(s) 1381, 2323, 3087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.