MD02G1259900.v1.1

alpha-1,4-glucosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000625
Physical Location & Seq
Forward (+)
31354108 .. 31354434
327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1259900.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGCGGAACCTGGCCCAAGTGTCATCAGCAATGTGTGTGAAAGTGTCAAGTGCATTGTTATCTTAATAACTGGCAAACCTATTGTAATCGAACCATATATTTCCTCGATTGATGCTCTGGTGGCAGCATGGTTGCCAGGCTCTGAAGGCCAAGGCATTACTGATGTCCTTTTTGGGGATCATGGATTCAGTGGAAAGCTTCCAAGAACATGGTTCAGAACTGTCGATCAACTCCCAATGAATGTTGGTGATTCAAACCATGATCCACTTTTCCCGTTTGGGTTCGGACTGGAAACTGAGTCAGTAAAGGAGCTTGTAACAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_31

Protein Analysis

109

Amino Acids

11.66

Weight (kDa)

4.63

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3_C PF01915 7 - 98 1.3e-22 Glycosyl hydrolase family 3 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000625)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47010 AT3G47040 AT3G47050
fragaria_vesca FvH4_1g28352 FvH4_3g26960 FvH4_7g05180
malus_domestica MD02G1089300.v1.1 MD02G1259900.v1.1 MD14G1004400.v1.1
prunus_persica Prupe.2G065700_v2.0.a1 Prupe.2G092700_v2.0.a1 Prupe.7G007100_v2.0.a1 Prupe.7G007700_v2.0.a1
pyrus_communis pycom02g22100 pycom07g04670 pycom11g06820 pycom11g15190 pycom14g00200 pycom14g00230
rosa_chinensis RchiOBHm_Chr5g0049431 RchiOBHm_Chr5g0049441 RchiOBHm_Chr5g0079371 RchiOBHm_Chr6g0274311
rosa_laevigata RLG00000006403 RLG00000008403 RLG00000028847 RLG00000034618
rosa_multiflora Rmu_co8385859.1_g000001 Rmu_sc0000376.1_g000001 Rmu_sc0001307.1_g000017 Rmu_sc0001307.1_g000019 Rmu_sc0001900.1_g000031 Rmu_sc0002912.1_g000028 Rmu_sc0006084.1_g000018 Rmu_sc0006888.1_g000063 Rmu_sc0014052.1_g000002 Rmu_sc0014052.1_g000004 Rmu_sc0022931.1_g000004
rosa_roxburghii Rroxscaffold_1G00003570 Rroxscaffold_1G00032240 Rroxscaffold_1G00032250 Rroxscaffold_2G00103090 Rroxscaffold_4G00289090 Rroxscaffold_5G00336970
rosa_rugosa Rorug03G0185900 Rorug04G0020500 Rorug04G0020600 Rorug04G0020600 Rorug04G0020700.1 Rorug04G0020800.1 Rorug04G0020900 Rorug04G0020900 Rorug04G0020900 Rorug05G0248500 Rorug05G0248500 Rorug05G0248500 Rorug05G0248600 Rorug05G0465800
rosa_samantha Rh3CG363400 Rh4DG330500 Rh4DG330600 Rh5AG245600 Rh5AG516800 Rh5AG517600 Rh5AG519000 Rh5AG519500 Rh5BG335900 Rh5BG336000 Rh5BG540700 Rh5BG542700 Rh5DG348200 Rh5DG348300 Rh5DG551900 Rh6BG185500 Rh7DG265300
rosa_wichuraiana Rw1G018240 Rw4G028270 Rw5G030690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 186, 257
AcuI CTGAAG 1 cut(s) 165
AfiI CCNNNNNNNGG 1 cut(s) 175
AgsI TTSAA 1 cut(s) 255
AjnI CCWGG 2 cut(s) 10, 136
AluBI AGCT 2 cut(s) 199, 313
AluI AGCT 2 cut(s) 199, 313
AlwI GGATC 2 cut(s) 186, 257
AlwNI CAGNNNCTG 1 cut(s) 143
AoxI GGCC 2 cut(s) 13, 148
ApeKI GCWGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 1 cut(s) 260
BbvI GCAGC 1 cut(s) 137
BciT130I CCWGG 2 cut(s) 12, 138
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 2 cut(s) 12, 138
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 12, 138
BmsI GCATC 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 2 cut(s) 76, 294
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 2 cut(s) 12, 138
BseDI CCNNGG 1 cut(s) 151
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 2 cut(s) 76, 294
BseXI GCAGC 1 cut(s) 137
BshFI GGCC 2 cut(s) 15, 150
BslI CCNNNNNNNGG 1 cut(s) 175
BsnI GGCC 2 cut(s) 15, 150
Bsp143I GATC 3 cut(s) 178, 226, 262
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 15, 150
BspCNI CTCAG 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 2 cut(s) 186, 257
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 2 cut(s) 76, 294
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 3 cut(s) 178, 226, 262
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 2 cut(s) 12, 138
Bst4CI ACNGT 1 cut(s) 223
BstDEI CTNAG 1 cut(s) 297
BstKTI GATC 3 cut(s) 181, 229, 265
BstMBI GATC 3 cut(s) 178, 226, 262
BstMWI GCNNNNNNNGC 2 cut(s) 122, 147
BstNI CCWGG 2 cut(s) 12, 138
BstSCI CCNGG 2 cut(s) 10, 136
BstV1I GCAGC 1 cut(s) 137
BstXI CCANNNNNNTGG 1 cut(s) 210
BsuRI GGCC 2 cut(s) 15, 150
BtsIMutI CAGTG 1 cut(s) 196
CaiI CAGNNNCTG 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 14
CviAII CATG 4 cut(s) 129, 182, 210, 260
CviJI RGCY 5 cut(s) 15, 141, 150, 199, 313
CviKI_1 RGCY 5 cut(s) 15, 141, 150, 199, 313
DdeI CTNAG 1 cut(s) 297
DpnI GATC 3 cut(s) 180, 228, 264
DpnII GATC 3 cut(s) 178, 226, 262
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 165
EcoRII CCWGG 2 cut(s) 10, 136
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 132, 185, 213, 263
FaiI YATR 6 cut(s) 97, 99, 130, 183, 211, 261
FatI CATG 4 cut(s) 128, 181, 209, 259
Fnu4HI GCNGC 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 126
GluI GCNGC 1 cut(s) 126
HaeIII GGCC 2 cut(s) 15, 150
Hin1II CATG 4 cut(s) 132, 185, 213, 263
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 3 cut(s) 186, 251, 299
HphI GGTGA 1 cut(s) 260
Hpy188I TCNGA 3 cut(s) 145, 218, 287
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 147
HpyF3I CTNAG 1 cut(s) 297
Hsp92II CATG 4 cut(s) 132, 185, 213, 263
Kzo9I GATC 3 cut(s) 178, 226, 262
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 6 cut(s) 24, 57, 104, 123, 150, 275
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 1 cut(s) 103
MaeIII GTNAC 1 cut(s) 316
MalI GATC 3 cut(s) 180, 228, 264
MboI GATC 3 cut(s) 178, 226, 262
MlyI GAGTC 1 cut(s) 308
MnlI CCTC 1 cut(s) 115
MseI TTAA 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 12, 138
MvaI CCWGG 2 cut(s) 12, 138
MwoI GCNNNNNNNGC 2 cut(s) 122, 147
NdeII GATC 3 cut(s) 178, 226, 262
NlaIII CATG 4 cut(s) 132, 185, 213, 263
NlaIV GGNNCC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 186, 251
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
Psp6I CCWGG 2 cut(s) 10, 136
PspGI CCWGG 2 cut(s) 10, 136
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 14
PstNI CAGNNNCTG 1 cut(s) 143
SaqAI TTAA 1 cut(s) 65
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 3 cut(s) 178, 226, 262
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 2 cut(s) 12, 138
SetI ASST 5 cut(s) 13, 82, 201, 315, 326
SfaNI GCATC 1 cut(s) 103
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 2 cut(s) 10, 136
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 223
TaqI TCGA 3 cut(s) 90, 107, 225
TfiI GAWTC 2 cut(s) 186, 251
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TscAI CASTG 1 cut(s) 196
TseI GCWGC 1 cut(s) 125
TspDTI ATGAA 1 cut(s) 254
TspRI CASTG 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.