pycom02g22100

Lysosomal beta

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000625
Physical Location & Seq
Forward (+)
20231739 .. 20232793
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22100.1

Sequence Viewer

Length: 360 bp
ATGGCTTGGGTTTCGTTACCAGTGGTGGGTTTGTTTTTGGTTTGGTGGTGGATGGGTTTGGCTACGGTCAAGGCTGATTACATCATGTACAAAGATCCAACCCAACCAGCGGCTGCTCGAGTTGGGGACCTTCTTAGCAGGATGACTCTTGAAGAGAAGATTGGTCAGATGGTGCAGATTGATAGGAGTGTTGCCTCTATTGATGCTATGAAAACTTACTTCATTGGTAGTGTATTGAGCGGTGGTGGCAGTGCACCACTTCCAGAAGCTAGTGCTGAAGACTGGGTTAACATGATTAATGGATTTCAGAAGGGAGCTCTATCTAGTCGTTTGGGCATTCCGATCGATCTACGGCATTGA

Protein Analysis

120

Amino Acids

13.01

Weight (kDa)

5.69

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000625)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47010 AT3G47040 AT3G47050
fragaria_vesca FvH4_1g28352 FvH4_3g26960 FvH4_7g05180
malus_domestica MD02G1089300.v1.1 MD02G1259900.v1.1 MD14G1004400.v1.1
prunus_persica Prupe.2G065700_v2.0.a1 Prupe.2G092700_v2.0.a1 Prupe.7G007100_v2.0.a1 Prupe.7G007700_v2.0.a1
pyrus_communis pycom02g22100 pycom07g04670 pycom11g06820 pycom11g15190 pycom14g00200 pycom14g00230
rosa_chinensis RchiOBHm_Chr5g0049431 RchiOBHm_Chr5g0049441 RchiOBHm_Chr5g0079371 RchiOBHm_Chr6g0274311
rosa_laevigata RLG00000006403 RLG00000008403 RLG00000028847 RLG00000034618
rosa_multiflora Rmu_co8385859.1_g000001 Rmu_sc0000376.1_g000001 Rmu_sc0001307.1_g000017 Rmu_sc0001307.1_g000019 Rmu_sc0001900.1_g000031 Rmu_sc0002912.1_g000028 Rmu_sc0006084.1_g000018 Rmu_sc0006888.1_g000063 Rmu_sc0014052.1_g000002 Rmu_sc0014052.1_g000004 Rmu_sc0022931.1_g000004
rosa_roxburghii Rroxscaffold_1G00003570 Rroxscaffold_1G00032240 Rroxscaffold_1G00032250 Rroxscaffold_2G00103090 Rroxscaffold_4G00289090 Rroxscaffold_5G00336970
rosa_rugosa Rorug03G0185900 Rorug04G0020500 Rorug04G0020600 Rorug04G0020600 Rorug04G0020700.1 Rorug04G0020800.1 Rorug04G0020900 Rorug04G0020900 Rorug04G0020900 Rorug05G0248500 Rorug05G0248500 Rorug05G0248500 Rorug05G0248600 Rorug05G0465800
rosa_samantha Rh3CG363400 Rh4DG330500 Rh4DG330600 Rh5AG245600 Rh5AG516800 Rh5AG517600 Rh5AG519000 Rh5AG519500 Rh5BG335900 Rh5BG336000 Rh5BG540700 Rh5BG542700 Rh5DG348200 Rh5DG348300 Rh5DG551900 Rh6BG185500 Rh7DG265300
rosa_wichuraiana Rw1G018240 Rw4G028270 Rw5G030690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 240
AciI CCGC 2 cut(s) 110, 240
AclWI GGATC 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 26, 109
AgsI TTSAA 1 cut(s) 152
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AluBI AGCT 2 cut(s) 269, 317
AluI AGCT 2 cut(s) 269, 317
Alw21I GWGCWC 2 cut(s) 256, 319
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 113
Ama87I CYCGRG 1 cut(s) 117
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 1 cut(s) 113
AseI ATTAAT 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 2 cut(s) 256, 319
BbvI GCAGC 1 cut(s) 100
BccI CCATC 2 cut(s) 46, 163
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BfaI CTAG 2 cut(s) 270, 324
BisI GCNGC 2 cut(s) 111, 114
BlsI GCNGC 2 cut(s) 112, 115
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 1 cut(s) 128
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 193
BmuI ACTGGG 1 cut(s) 292
BpiI GAAGAC 1 cut(s) 285
Bsa29I ATCGAT 1 cut(s) 345
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 109
Bse1I ACTGG 2 cut(s) 20, 287
BseCI ATCGAT 1 cut(s) 345
BseGI GGATG 2 cut(s) 57, 147
BseLI CCNNNNNNNGG 2 cut(s) 26, 109
BseNI ACTGG 2 cut(s) 20, 287
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 1 cut(s) 100
BsgI GTGCAG 1 cut(s) 194
Bsh1285I CGRYCG 1 cut(s) 345
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 2 cut(s) 256, 319
BsiHKCI CYCGRG 1 cut(s) 117
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 26, 109
BsmFI GGGAC 1 cut(s) 140
BsmI GAATGC 1 cut(s) 336
BsoBI CYCGRG 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 256, 319
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 3 cut(s) 94, 342, 346
BspACI CCGC 2 cut(s) 110, 240
BspDI ATCGAT 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 87
BsrI ACTGG 2 cut(s) 20, 287
BssMI GATC 3 cut(s) 94, 342, 346
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 57, 147
BstKTI GATC 3 cut(s) 97, 345, 349
BstMBI GATC 3 cut(s) 94, 342, 346
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 345
BsuTUI ATCGAT 1 cut(s) 345
BtsCI GGATG 2 cut(s) 57, 147
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 27, 256
CaiI CAGNNNCTG 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 127
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 1 cut(s) 88
CviAII CATG 2 cut(s) 85, 292
CviJI RGCY 6 cut(s) 5, 62, 74, 113, 269, 317
CviKI_1 RGCY 6 cut(s) 5, 62, 74, 113, 269, 317
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 1 cut(s) 134
DpnI GATC 3 cut(s) 96, 344, 348
DpnII GATC 3 cut(s) 94, 342, 346
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Ecl136II GAGCTC 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 297
Eco88I CYCGRG 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 319
FaeI CATG 2 cut(s) 88, 295
FaiI YATR 3 cut(s) 86, 209, 293
FaqI GGGAC 1 cut(s) 140
FatI CATG 2 cut(s) 84, 291
Fnu4HI GCNGC 2 cut(s) 111, 114
FokI GGATG 2 cut(s) 64, 154
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 2 cut(s) 111, 114
FspBI CTAG 2 cut(s) 270, 324
GluI GCNGC 2 cut(s) 111, 114
Hin1II CATG 2 cut(s) 88, 295
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 1 cut(s) 145
HpaI GTTAAC 1 cut(s) 289
Hpy166II GTNNAC 2 cut(s) 254, 289
Hpy188I TCNGA 3 cut(s) 168, 309, 342
Hpy188III TCNNGA 2 cut(s) 149, 263
Hpy8I GTNNAC 2 cut(s) 254, 289
HpyAV CCTTC 2 cut(s) 140, 304
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4V TGCA 2 cut(s) 175, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 134
Hsp92II CATG 2 cut(s) 88, 295
KspAI GTTAAC 1 cut(s) 289
Kzo9I GATC 3 cut(s) 94, 342, 346
LmnI GCTCC 1 cut(s) 314
LpnPI CCDG 5 cut(s) 33, 120, 124, 268, 276
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 1 cut(s) 193
MaeI CTAG 2 cut(s) 270, 324
MaeIII GTNAC 1 cut(s) 15
MalI GATC 3 cut(s) 96, 344, 348
MbiI CCGCTC 1 cut(s) 240
MboI GATC 3 cut(s) 94, 342, 346
MboII GAAGA 3 cut(s) 164, 169, 290
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 2 cut(s) 256, 319
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 1 cut(s) 205
MseI TTAA 2 cut(s) 288, 297
MspA1I CMGCKG 1 cut(s) 110
Mva1269I GAATGC 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 3 cut(s) 94, 342, 346
NlaIII CATG 2 cut(s) 88, 295
NlaIV GGNNCC 1 cut(s) 128
PaeR7I CTCGAG 1 cut(s) 117
PctI GAATGC 1 cut(s) 336
PkrI GCNGC 2 cut(s) 112, 115
Ple19I CGATCG 1 cut(s) 345
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 127
PshBI ATTAAT 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 127
PspPPI RGGWCCY 1 cut(s) 127
PspXI VCTCGAGB 1 cut(s) 117
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 94
PvuI CGATCG 1 cut(s) 345
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SacI GAGCTC 1 cut(s) 319
SaqAI TTAA 2 cut(s) 288, 297
SatI GCNGC 2 cut(s) 111, 114
Sau3AI GATC 3 cut(s) 94, 342, 346
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 1 cut(s) 139
SduI GDGCHC 2 cut(s) 256, 319
SetI ASST 3 cut(s) 132, 271, 319
SfaNI GCATC 1 cut(s) 193
Sfr274I CTCGAG 1 cut(s) 117
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 117
SmoI CTYRAG 1 cut(s) 117
SsiI CCGC 2 cut(s) 110, 240
SspMI CTAG 2 cut(s) 270, 324
SstI GAGCTC 1 cut(s) 319
TaaI ACNGT 1 cut(s) 67
TaqI TCGA 2 cut(s) 118, 345
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 113
Tru1I TTAA 2 cut(s) 288, 297
Tru9I TTAA 2 cut(s) 288, 297
TscAI CASTG 2 cut(s) 27, 256
TseI GCWGC 1 cut(s) 113
TspDTI ATGAA 2 cut(s) 211, 224
TspRI CASTG 2 cut(s) 27, 256
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 127
VspI ATTAAT 1 cut(s) 297
XhoI CTCGAG 1 cut(s) 117
XspI CTAG 2 cut(s) 270, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.