MD02G1315300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001007
Physical Location & Seq
Forward (+)
37013145 .. 37016328
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1315300.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGAAATTTGACCCTATTTCAAAACCTTCGGGCATCTCCTTCTCCTTCACCTTCACCTTCGCTTCCCTTGGAACTGCGCCGCTGCTCCTTCCCTTGGAACCCTCGGGCATCTCTTTTCCAGCCTCGCCGCCGCTGCTCTTCATCTCCTTACAAGCTGTGGATGAACAATTCGAGGAGCAATCTGAGCATTCCGAAAGTGGAATGATGCTTCAATTGAGAACTCAACGCAAAACTCGTGGGCCTGGCAAAATAAAATGGGCCAGCAAAGATGGGAGAGGAAAAGAATATTGTGAATTTGGTCCGACTGGGAAATGCATTAGTGCCAATAGTGTCAACTTTTCTAAGTTGATTGCGTCTGAGGTCAAGGATCCCAGAAATCTTCCTTTGAAGACTGATTGGCGATTGATTGATGAAGATGTCAAGGAAGTTTTCTGGAATACAATACGTGACACTATTGCTTTTCGTGATGAAGATCTGGTTAAAATGCCCCTCATCCGCCTTATGACGCTTTCTATTGCCGAGCATGCACATAAAGAATATAGGAATTATTTGAAGAAAATCTTCTACAGTCGCAGACAAGGGGAGGATCGCTTACAGTGTCCTCCTAATGTGGATCCCGGCCAGTGGGTGGAGATGGTGCGATATTGGGATAATCCAAAAACGATGGAGAAGGCTGAAAAAAATAGGATCAATCGTCAAACCAAAACTATGAACCACACAACAGGATCAAAGACATTTGTCAGAGTTCGGGAAGAATATAGGAAGGAGCATGGAGAAGAACCAGATCCTATTGCTTTCTTTCGCATGACCCATTCACGGAAAGATAGTACATGGGTTGATGAAACGGCTCAACAAAAAGGGGCATCTATGGAGAGTGTCGTTCAATCTAAGGTTGAGGATGGAGGAGAAGACACTCCTGAACTTCGAACTCAAGTCTATGTCGAGGAGATGGGTCCTGAGAATAGGAATAGAGTTCGAGGTTATGGACATGGGGTGACACCTGTGATGGTGCCGTATGCTGCTACTGGTTCATCAAAGAGATCATCGAGTAGTCGAGAAGCCATTGTGCAGGCTGAGAACCTTAAATTGAGGAGGAATGAAGAGGCTCGTACTACAGAGTTAGATGAGTTAAAGACCCAGCTTCAGCAGATACAGGCGACGCAGCAGCAGATGAACCAGCAGCAGCAGATGATGATGATGATGTTTATGCAAGGTATGCAGCAGCCACAGACTTACCTGCCATGCCCCCTGGCACCACAGCATTCGGAGAGAGAGGGAGGGAGAAGGGGGGAGAGCAGTCGTTATCTTCAGCAAATACAGGCAGCGCAGCAGCAGATGAACCAGCAGCAGATGATGATGATGATGTTTAGGCAGGGTATGCAGCAGCCACAGACTTACCCGCACAGCCCTCAGGCATCACAGCATTCAGAGAGAGAGGGAGGGAGAAGGAAGGAGAGCAGTCGCAAAAAGATGAGAAGGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

496

Amino Acids

57.26

Weight (kDa)

9.35

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 206 - 334 1.5e-29 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1245
AccB1I GGYRCC 2 cut(s) 1009, 1252
AccB7I CCANNNNNTGG 1 cut(s) 628
AciI CCGC 5 cut(s) 80, 128, 131, 496, 1400
AclWI GGATC 8 cut(s) 362, 375, 594, 608, 621, 695, 733, 779
AcoI YGGCCR 1 cut(s) 619
AcsI RAATTY 2 cut(s) 5, 293
AcuI CTGAAG 2 cut(s) 1127, 1292
AfaI GTAC 2 cut(s) 829, 1111
AfiI CCNNNNNNNGG 4 cut(s) 94, 624, 628, 816
AgsI TTSAA 5 cut(s) 21, 212, 388, 553, 884
AjnI CCWGG 2 cut(s) 241, 1248
AluBI AGCT 2 cut(s) 155, 1141
AluI AGCT 2 cut(s) 155, 1141
AlwI GGATC 8 cut(s) 362, 375, 594, 608, 621, 695, 733, 779
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 3 cut(s) 239, 258, 619
ApoI RAATTY 2 cut(s) 5, 293
Asp700I GAANNNNTTC 1 cut(s) 560
AspLEI GCGC 2 cut(s) 79, 1327
AspS9I GGNCC 4 cut(s) 239, 258, 299, 953
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 3 cut(s) 40, 46, 1006
AsuII TTCGAA 1 cut(s) 925
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 2 cut(s) 299, 953
AxyI CCTNAGG 1 cut(s) 1410
BamHI GGATCC 2 cut(s) 367, 613
BanI GGYRCC 2 cut(s) 1009, 1252
BauI CACGAG 1 cut(s) 234
BbsI GAAGAC 2 cut(s) 395, 915
BccI CCATC 6 cut(s) 263, 628, 658, 893, 943, 1000
BceAI ACGGC 2 cut(s) 861, 997
BciT130I CCWGG 2 cut(s) 243, 1250
BcnI CCSGG 1 cut(s) 618
BfmI CTRYAG 2 cut(s) 565, 1113
BfuAI ACCTGC 1 cut(s) 1245
BglII AGATCT 1 cut(s) 472
Bme1390I CCNGG 3 cut(s) 243, 618, 1250
Bme18I GGWCC 2 cut(s) 299, 953
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 4 cut(s) 239, 258, 299, 953
BmiI GGNNCC 6 cut(s) 99, 369, 615, 954, 1011, 1254
BmrFI CCNGG 3 cut(s) 243, 618, 1250
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 5 cut(s) 42, 117, 195, 872, 1424
BmuI ACTGGG 1 cut(s) 315
BoxI GACNNNNGTC 1 cut(s) 737
BpiI GAAGAC 2 cut(s) 395, 915
Bpu14I TTCGAA 1 cut(s) 925
BpuEI CTTGAG 1 cut(s) 915
BpuMI CCSGG 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 446
BsaBI GATNNNNATC 1 cut(s) 471
BsaJI CCNNGG 4 cut(s) 67, 93, 102, 1248
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 624, 628, 816
Bse1I ACTGG 3 cut(s) 310, 622, 1030
Bse21I CCTNAGG 1 cut(s) 1410
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 2 cut(s) 243, 1250
BseDI CCNNGG 4 cut(s) 67, 93, 102, 1248
BseGI GGATG 3 cut(s) 166, 492, 904
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 4 cut(s) 94, 624, 628, 816
BseMII CTCAG 5 cut(s) 174, 348, 948, 1065, 1424
BseNI ACTGG 3 cut(s) 310, 622, 1030
BseRI GAGGAG 4 cut(s) 188, 918, 959, 1105
BseYI CCCAGC 1 cut(s) 1137
BsgI GTGCAG 1 cut(s) 1088
BshFI GGCC 3 cut(s) 241, 260, 621
BshNI GGYRCC 2 cut(s) 1009, 1252
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 1 cut(s) 618
BslI CCNNNNNNNGG 4 cut(s) 94, 624, 628, 816
BsmI GAATGC 3 cut(s) 187, 1261, 1423
BsnI GGCC 3 cut(s) 241, 260, 621
BsoBI CYCGRG 1 cut(s) 103
Bsp119I TTCGAA 1 cut(s) 925
Bsp143I GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
BspACI CCGC 5 cut(s) 80, 128, 131, 496, 1400
BspANI GGCC 3 cut(s) 241, 260, 621
BspCNI CTCAG 5 cut(s) 175, 349, 949, 1066, 1423
BspLI GGNNCC 6 cut(s) 99, 369, 615, 954, 1011, 1254
BspMI ACCTGC 1 cut(s) 1245
BspPI GGATC 8 cut(s) 362, 375, 594, 608, 621, 695, 733, 779
BspQI GCTCTTC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 925
BspT107I GGYRCC 2 cut(s) 1009, 1252
BsrI ACTGG 3 cut(s) 310, 622, 1030
BssECI CCNNGG 4 cut(s) 67, 93, 102, 1248
BssMI GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
BssSI CACGAG 1 cut(s) 234
BssT1I CCWWGG 2 cut(s) 67, 93
Bst2BI CACGAG 1 cut(s) 234
Bst2UI CCWGG 2 cut(s) 243, 1250
Bst4CI ACNGT 2 cut(s) 569, 597
Bst6I CTCTTC 2 cut(s) 143, 1095
BstAPI GCANNNNNTGC 2 cut(s) 1216, 1378
BstBAI YACGTR 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 925
BstC8I GCNNGC 3 cut(s) 262, 525, 1071
BstDEI CTNAG 7 cut(s) 183, 342, 357, 888, 957, 1074, 1410
BstF5I GGATG 3 cut(s) 166, 492, 904
BstHHI GCGC 2 cut(s) 79, 1327
BstKTI GATC 8 cut(s) 370, 475, 589, 616, 690, 728, 787, 1043
BstMBI GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
BstMWI GCNNNNNNNGC 5 cut(s) 133, 184, 524, 1216, 1378
BstNI CCWGG 2 cut(s) 243, 1250
BstNSI RCATGY 1 cut(s) 527
BstPAI GACNNNNGTC 1 cut(s) 737
BstSCI CCNGG 3 cut(s) 241, 616, 1248
BstSFI CTRYAG 2 cut(s) 565, 1113
BstV2I GAAGAC 2 cut(s) 395, 915
BstX2I RGATCY 4 cut(s) 367, 472, 613, 784
BstYI RGATCY 4 cut(s) 367, 472, 613, 784
Bsu36I CCTNAGG 1 cut(s) 1410
BsuRI GGCC 3 cut(s) 241, 260, 621
BtsCI GGATG 3 cut(s) 166, 492, 904
BtsIMutI CAGTG 2 cut(s) 602, 629
BveI ACCTGC 1 cut(s) 1245
Cac8I GCNNGC 3 cut(s) 262, 525, 1071
CfoI GCGC 2 cut(s) 79, 1327
Cfr13I GGNCC 4 cut(s) 239, 258, 299, 953
CseI GACGC 3 cut(s) 342, 514, 1168
Csp6I GTAC 2 cut(s) 828, 1110
CspCI CAANNNNNGTGG 2 cut(s) 217, 252
CviAII CATG 6 cut(s) 524, 770, 805, 831, 989, 1242
CviQI GTAC 2 cut(s) 828, 1110
DdeI CTNAG 7 cut(s) 183, 342, 357, 888, 957, 1074, 1410
DpnI GATC 8 cut(s) 369, 474, 588, 615, 689, 727, 786, 1042
DpnII GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
EaeI YGGCCR 1 cut(s) 619
Eam1104I CTCTTC 2 cut(s) 143, 1095
EarI CTCTTC 2 cut(s) 143, 1095
EciI GGCGGA 1 cut(s) 485
Eco130I CCWWGG 2 cut(s) 67, 93
Eco47I GGWCC 2 cut(s) 299, 953
Eco57I CTGAAG 2 cut(s) 1127, 1292
Eco81I CCTNAGG 1 cut(s) 1410
Eco88I CYCGRG 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 953
EcoRII CCWGG 2 cut(s) 241, 1248
EcoT14I CCWWGG 2 cut(s) 67, 93
EcoT22I ATGCAT 1 cut(s) 317
ErhI CCWWGG 2 cut(s) 67, 93
FaeI CATG 6 cut(s) 527, 773, 808, 834, 992, 1245
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FatI CATG 6 cut(s) 523, 769, 804, 830, 988, 1241
FauI CCCGC 1 cut(s) 1407
FokI GGATG 3 cut(s) 173, 479, 911
GlaI GCGC 2 cut(s) 78, 1326
GsaI CCCAGC 1 cut(s) 1141
HaeIII GGCC 3 cut(s) 241, 260, 621
HapII CCGG 1 cut(s) 618
HgaI GACGC 3 cut(s) 342, 514, 1168
HhaI GCGC 2 cut(s) 79, 1327
Hin1II CATG 6 cut(s) 527, 773, 808, 834, 992, 1245
Hin6I GCGC 2 cut(s) 77, 1325
HinP1I GCGC 2 cut(s) 77, 1325
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HpaII CCGG 1 cut(s) 618
HphI GGTGA 3 cut(s) 40, 46, 1006
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 7 cut(s) 184, 193, 303, 358, 743, 1267, 1429
Hpy188III TCNNGA 6 cut(s) 433, 464, 749, 917, 956, 1055
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 1162
HpyCH4III ACNGT 2 cut(s) 569, 597
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 6 cut(s) 315, 527, 1069, 1210, 1219, 1381
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 184, 524, 1216, 1378
HpyF3I CTNAG 7 cut(s) 183, 342, 357, 888, 957, 1074, 1410
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 6 cut(s) 527, 773, 808, 834, 992, 1245
HspAI GCGC 2 cut(s) 77, 1325
Kzo9I GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 3 cut(s) 90, 175, 766
LweI GCATC 5 cut(s) 42, 117, 195, 872, 1424
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 2 cut(s) 446, 994
MalI GATC 8 cut(s) 369, 474, 588, 615, 689, 727, 786, 1042
MboI GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
MfeI CAATTG 1 cut(s) 212
MflI RGATCY 4 cut(s) 367, 472, 613, 784
MluCI AATT 6 cut(s) 5, 167, 212, 293, 544, 1085
MmeI TCCRAC 1 cut(s) 326
Mph1103I ATGCAT 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 560
MseI TTAA 3 cut(s) 480, 1083, 1130
MspA1I CMGCKG 2 cut(s) 82, 133
MspI CCGG 1 cut(s) 618
MspR9I CCNGG 3 cut(s) 243, 618, 1250
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 3 cut(s) 187, 1261, 1423
MvaI CCWGG 2 cut(s) 243, 1250
MwoI GCNNNNNNNGC 5 cut(s) 133, 184, 524, 1216, 1378
NciI CCSGG 1 cut(s) 618
NdeII GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
NlaIII CATG 6 cut(s) 527, 773, 808, 834, 992, 1245
NlaIV GGNNCC 6 cut(s) 99, 369, 615, 954, 1011, 1254
NmeAIII GCCGAG 1 cut(s) 544
NmuCI GTSAC 2 cut(s) 446, 994
NsiI ATGCAT 1 cut(s) 317
NspI RCATGY 1 cut(s) 527
NspV TTCGAA 1 cut(s) 925
PaeI GCATGC 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PctI GAATGC 3 cut(s) 187, 1261, 1423
PdmI GAANNNNTTC 1 cut(s) 560
PflMI CCANNNNNTGG 1 cut(s) 628
Ppu21I YACGTR 1 cut(s) 446
PpuMI RGGWCCY 1 cut(s) 953
PshAI GACNNNNGTC 1 cut(s) 737
Psp5II RGGWCCY 1 cut(s) 953
Psp6I CCWGG 2 cut(s) 241, 1248
PspFI CCCAGC 1 cut(s) 1137
PspGI CCWGG 2 cut(s) 241, 1248
PspN4I GGNNCC 6 cut(s) 99, 369, 615, 954, 1011, 1254
PspPI GGNCC 4 cut(s) 239, 258, 299, 953
PspPPI RGGWCCY 1 cut(s) 953
PsuI RGATCY 4 cut(s) 367, 472, 613, 784
RsaI GTAC 2 cut(s) 829, 1111
RsaNI GTAC 2 cut(s) 828, 1110
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 3 cut(s) 480, 1083, 1130
Sau3AI GATC 8 cut(s) 367, 472, 586, 613, 687, 725, 784, 1040
Sau96I GGNCC 4 cut(s) 239, 258, 299, 953
ScrFI CCNGG 3 cut(s) 243, 618, 1250
SfaNI GCATC 5 cut(s) 42, 117, 195, 872, 1424
SfcI CTRYAG 2 cut(s) 565, 1113
SfuI TTCGAA 1 cut(s) 925
SinI GGWCC 2 cut(s) 299, 953
SmlI CTYRAG 1 cut(s) 930
SmoI CTYRAG 1 cut(s) 930
SphI GCATGC 1 cut(s) 527
Sse9I AATT 6 cut(s) 5, 167, 212, 293, 544, 1085
SsiI CCGC 5 cut(s) 80, 128, 131, 496, 1400
SspI AATATT 1 cut(s) 287
StyD4I CCNGG 3 cut(s) 241, 616, 1248
StyI CCWWGG 2 cut(s) 67, 93
TaaI ACNGT 2 cut(s) 569, 597
TaiI ACGT 1 cut(s) 448
TaqI TCGA 6 cut(s) 171, 925, 942, 976, 1046, 1054
TasI AATT 6 cut(s) 5, 167, 212, 293, 544, 1085
TatI WGTACW 1 cut(s) 827
TauI GCSGC 3 cut(s) 82, 130, 133
Tru1I TTAA 3 cut(s) 480, 1083, 1130
Tru9I TTAA 3 cut(s) 480, 1083, 1130
TscAI CASTG 2 cut(s) 602, 629
TseFI GTSAC 2 cut(s) 446, 994
Tsp45I GTSAC 2 cut(s) 446, 994
TspGWI ACGGA 1 cut(s) 832
TspRI CASTG 2 cut(s) 602, 629
Van91I CCANNNNNTGG 1 cut(s) 628
VpaK11BI GGWCC 2 cut(s) 299, 953
XapI RAATTY 2 cut(s) 5, 293
XceI RCATGY 1 cut(s) 527
XmnI GAANNNNTTC 1 cut(s) 560
Zsp2I ATGCAT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.