Rroxscaffold_2G00099520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001007
Physical Location & Seq
Reverse (-)
21225155 .. 21226111
957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099520.1

Sequence Viewer

Length: 681 bp
ATGTGTGGAGTGTACAAGATAGAGAGTTGGAGCTGTTTCCAGCCGACTTCGATTAGCGCCGCCCGCTTCACCGACTTTGCCCTCAACCATTCTCGTGCCACCAAGAGCCGTGATCAAGAAAACTTTGGTGCCGTAAAGCTCACCGCGAGCATATGTTGCGTTGATGGGACCCGAGAAACGTCACGTAGTTTGCGCTATGGACTAGGTGTAATACCCGATATGGTACCTTATCTTCAAATTGATGGTAGCTCATCATCACACAGATCACATGGAAGACACTATGCTCACTTGCCGTCACAATTTATGGTATTAGAGTCCAAGTACCAACAACAACAAGAGCAAGCCCAAGTGCAACAAGTTCAAACTCAGGAACAACTCTTGCAAACCCAAAGAGAATTGGCAATGTTAAAGAAAATGATACAATCCCAGCCATCTACTCTATCTTCACAACAATCTTCTCAGCCTATGCAGCAACCATCCTTCCAAATGCCTTCTTATTCTCCTCAATTGTACCGGCCTCAACCACAGATCTATCCTCAAATGCCTTATCAGCTGCAACATGCATATCCTACCACCGGACTAACCGGATTGCTTATGGGAGATACAAGTGTCCTAGAAACTGATTTGTTGAACCAACTTAGAGCTTCATCGAATCAAAGCTCTCGTGGATCCGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

25.5

Weight (kDa)

8.34

Isoelectric Point (pI)

68.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 223
AccB1I GGYRCC 2 cut(s) 128, 223
AccII CGCG 1 cut(s) 146
AciI CCGC 3 cut(s) 60, 64, 144
AclWI GGATC 2 cut(s) 663, 676
AfaI GTAC 4 cut(s) 14, 225, 323, 512
AfiI CCNNNNNNNGG 1 cut(s) 575
AgsI TTSAA 3 cut(s) 236, 362, 631
AluBI AGCT 6 cut(s) 33, 139, 249, 553, 644, 660
AluI AGCT 6 cut(s) 33, 139, 249, 553, 644, 660
AlwI GGATC 2 cut(s) 663, 676
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 1 cut(s) 515
ApeKI GCWGC 2 cut(s) 469, 553
Asp718I GGTACC 1 cut(s) 223
AspLEI GCGC 2 cut(s) 59, 195
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 61, 133
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 1 cut(s) 168
BaeI ACNNNNGTAYC 2 cut(s) 594, 627
BamHI GGATCC 1 cut(s) 668
BanI GGYRCC 2 cut(s) 128, 223
BarI GAAGNNNNNNTAC 2 cut(s) 216, 248
BauI CACGAG 2 cut(s) 93, 663
BbsI GAAGAC 1 cut(s) 280
BbvI GCAGC 2 cut(s) 481, 540
BccI CCATC 4 cut(s) 158, 236, 439, 484
BceAI ACGGC 3 cut(s) 93, 116, 277
BclI TGATCA 1 cut(s) 112
BfaI CTAG 2 cut(s) 203, 614
BfoI RGCGCY 1 cut(s) 60
BglII AGATCT 1 cut(s) 528
BisI GCNGC 3 cut(s) 60, 470, 554
BlsI GCNGC 3 cut(s) 61, 471, 555
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 5 cut(s) 130, 169, 170, 225, 670
BpiI GAAGAC 1 cut(s) 280
BsaAI YACGTR 1 cut(s) 185
BsaWI WCCGGW 2 cut(s) 575, 584
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 408
BseGI GGATG 1 cut(s) 476
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 380, 473
BseRI GAGGAG 1 cut(s) 492
BseXI GCAGC 2 cut(s) 481, 540
BseYI CCCAGC 1 cut(s) 426
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 1 cut(s) 517
BshNI GGYRCC 2 cut(s) 128, 223
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 3 cut(s) 514, 576, 585
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 1 cut(s) 517
BsoBI CYCGRG 1 cut(s) 171
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 4 cut(s) 112, 263, 528, 668
BspACI CCGC 3 cut(s) 60, 64, 144
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 2 cut(s) 379, 472
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 5 cut(s) 130, 169, 170, 225, 670
BspPI GGATC 2 cut(s) 663, 676
BspT107I GGYRCC 2 cut(s) 128, 223
BsrDI GCAATG 1 cut(s) 408
BsrFI RCCGGY 1 cut(s) 513
BsrGI TGTACA 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 513
BssMI GATC 4 cut(s) 112, 263, 528, 668
BssSI CACGAG 2 cut(s) 93, 663
Bst2BI CACGAG 2 cut(s) 93, 663
BstAPI GCANNNNNTGC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 12
BstBAI YACGTR 1 cut(s) 185
BstC8I GCNNGC 3 cut(s) 64, 148, 342
BstDEI CTNAG 3 cut(s) 366, 459, 638
BstF5I GGATG 1 cut(s) 476
BstFNI CGCG 1 cut(s) 146
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 195
BstKTI GATC 4 cut(s) 115, 266, 531, 671
BstMBI GATC 4 cut(s) 112, 263, 528, 668
BstMWI GCNNNNNNNGC 4 cut(s) 63, 156, 469, 550
BstNSI RCATGY 1 cut(s) 563
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 481, 540
BstV2I GAAGAC 1 cut(s) 280
BstX2I RGATCY 2 cut(s) 528, 668
BstYI RGATCY 2 cut(s) 528, 668
BsuRI GGCC 1 cut(s) 517
BtsCI GGATG 1 cut(s) 476
Cac8I GCNNGC 3 cut(s) 64, 148, 342
CfoI GCGC 2 cut(s) 59, 195
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 1 cut(s) 168
Csp6I GTAC 4 cut(s) 13, 224, 322, 511
CviAII CATG 2 cut(s) 269, 560
CviQI GTAC 4 cut(s) 13, 224, 322, 511
DdeI CTNAG 3 cut(s) 366, 459, 638
DpnI GATC 4 cut(s) 114, 265, 530, 670
DpnII GATC 4 cut(s) 112, 263, 528, 668
Eco47I GGWCC 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 171
EcoO109I RGGNCCY 1 cut(s) 168
EcoT22I ATGCAT 1 cut(s) 565
FaeI CATG 2 cut(s) 272, 563
FaqI GGGAC 1 cut(s) 181
FatI CATG 2 cut(s) 268, 559
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 1 cut(s) 152
FbaI TGATCA 1 cut(s) 112
Fnu4HI GCNGC 3 cut(s) 60, 470, 554
FokI GGATG 1 cut(s) 463
Fsp4HI GCNGC 3 cut(s) 60, 470, 554
FspBI CTAG 2 cut(s) 203, 614
GlaI GCGC 2 cut(s) 58, 194
GluI GCNGC 3 cut(s) 60, 470, 554
GsaI CCCAGC 1 cut(s) 430
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 1 cut(s) 517
HapII CCGG 3 cut(s) 514, 576, 585
HhaI GCGC 2 cut(s) 59, 195
Hin1II CATG 2 cut(s) 272, 563
Hin6I GCGC 2 cut(s) 57, 193
HinP1I GCGC 2 cut(s) 57, 193
HinfI GANTC 2 cut(s) 314, 652
HpaII CCGG 3 cut(s) 514, 576, 585
HphI GGTGA 2 cut(s) 61, 133
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 1 cut(s) 673
Hpy188III TCNNGA 2 cut(s) 116, 368
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 490, 501
HpyCH4IV ACGT 2 cut(s) 179, 184
HpyCH4V TGCA 5 cut(s) 352, 382, 469, 556, 563
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 156, 469, 550
HpyF3I CTNAG 3 cut(s) 366, 459, 638
HpySE526I ACGT 2 cut(s) 179, 184
Hsp92II CATG 2 cut(s) 272, 563
HspAI GCGC 2 cut(s) 57, 193
KflI GGGWCCC 1 cut(s) 168
KpnI GGTACC 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 4 cut(s) 112, 263, 528, 668
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 6 cut(s) 53, 353, 440, 527, 589, 598
Lsp1109I GCAGC 2 cut(s) 481, 540
MaeI CTAG 2 cut(s) 203, 614
MaeII ACGT 2 cut(s) 179, 184
MaeIII GTNAC 2 cut(s) 180, 294
MalI GATC 4 cut(s) 114, 265, 530, 670
MboI GATC 4 cut(s) 112, 263, 528, 668
MboII GAAGA 4 cut(s) 224, 285, 435, 447
MfeI CAATTG 1 cut(s) 506
MflI RGATCY 2 cut(s) 528, 668
MluCI AATT 4 cut(s) 237, 299, 395, 506
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 8
MnlI CCTC 4 cut(s) 92, 513, 528, 546
Mph1103I ATGCAT 1 cut(s) 565
MseI TTAA 1 cut(s) 407
MslI CAYNNNNRTG 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 3 cut(s) 514, 576, 585
MunI CAATTG 1 cut(s) 506
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 4 cut(s) 63, 156, 469, 550
NdeI CATATG 1 cut(s) 152
NdeII GATC 4 cut(s) 112, 263, 528, 668
NlaIII CATG 2 cut(s) 272, 563
NlaIV GGNNCC 5 cut(s) 130, 169, 170, 225, 670
NmuCI GTSAC 2 cut(s) 180, 294
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 563
PfeI GAWTC 1 cut(s) 652
PkrI GCNGC 3 cut(s) 61, 471, 555
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 185
PpuMI RGGWCCY 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 426
PspN4I GGNNCC 5 cut(s) 130, 169, 170, 225, 670
PspPI GGNCC 1 cut(s) 168
PspPPI RGGWCCY 1 cut(s) 168
PsuI RGATCY 2 cut(s) 528, 668
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 4 cut(s) 14, 225, 323, 512
RsaNI GTAC 4 cut(s) 13, 224, 322, 511
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 3 cut(s) 60, 470, 554
Sau3AI GATC 4 cut(s) 112, 263, 528, 668
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 323
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 93
Sse9I AATT 4 cut(s) 237, 299, 395, 506
SsiI CCGC 3 cut(s) 60, 64, 144
SspMI CTAG 2 cut(s) 203, 614
TaiI ACGT 2 cut(s) 182, 187
TaqI TCGA 2 cut(s) 50, 650
TasI AATT 4 cut(s) 237, 299, 395, 506
TatI WGTACW 1 cut(s) 12
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 1 cut(s) 652
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TseFI GTSAC 2 cut(s) 180, 294
TseI GCWGC 2 cut(s) 469, 553
Tsp45I GTSAC 2 cut(s) 180, 294
TspDTI ATGAA 1 cut(s) 636
VpaK11BI GGWCC 1 cut(s) 168
XceI RCATGY 1 cut(s) 563
XspI CTAG 2 cut(s) 203, 614
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.