MD03G1064000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001007
Physical Location & Seq
Reverse (-)
5129969 .. 5131002
1034 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1064000.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGGTTTTGAGACAAGGAATAAGGTACGAGGCTATGGTCATGGGGTGATACCAGAGATGGTGTCATATGCAAGCTCTTCAGGTTCCAGGTCATCCAGGAGTTCATCCAAAGGTGCATTTGCCAAGGTGGTAGCTGAGAACAATGAATTGAGGAGGAGGGGGGAAGAAGCCGCCAGAAAGTTAGCAGAGGCAACGGTTAAGCTCGAGAAAACAAAGACCTCGCAGCAGCAGGCAATGAATCAGCAGATGCTGATGAATCAACATCAAATAATGTTGTGGCTTCACAGCATTCGGCCACCACCCAGCCTTCAGGCATCACAGCATTCGGCCCCAATGCAGCCACCTGCGCCTGTATATCCCATGCAACATATGCCTTTTCAGCCCCAGTATCCAATGTCAGTATATCGACCCCAGATGACTTCTCCAGGTGGCGCACATATTTCTCAAGGTGGTTTCAATGGTGGATTATCATCGGGTTTCTTATCTGGGTTGATGATGGGTAGTTTTGATGAGGATGTGATGAGGTATTTGGAAGCACATGGGTTGGCAGAAGGTTTTGGATCACAGGGAGCATCGGCAGAAGGCGTTGGTTCGCAAGGAGGGTCGGCAGAAGGCGTTGGTTCGCAAGGAGGGTCGGCAGAAGGCTTTGGAGCGCAGGGTGGTTTGGGGGAAGGCTCGGGATCTATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

231

Amino Acids

24.21

Weight (kDa)

7.08

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 1 cut(s) 352
AciI CCGC 1 cut(s) 171
AclWI GGATC 2 cut(s) 568, 688
AcoI YGGCCR 1 cut(s) 293
AcuI CTGAAG 2 cut(s) 63, 293
AfaI GTAC 1 cut(s) 27
AgsI TTSAA 2 cut(s) 457, 689
AjnI CCWGG 3 cut(s) 86, 95, 424
AluBI AGCT 3 cut(s) 75, 134, 202
AluI AGCT 3 cut(s) 75, 134, 202
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 2 cut(s) 568, 688
AlwNI CAGNNNCTG 1 cut(s) 250
Ama87I CYCGRG 2 cut(s) 203, 676
AoxI GGCC 2 cut(s) 293, 327
ApeKI GCWGC 3 cut(s) 223, 226, 337
AspLEI GCGC 3 cut(s) 349, 434, 655
AspS9I GGNCC 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 2 cut(s) 203, 676
BbvI GCAGC 3 cut(s) 235, 238, 349
BccI CCATC 2 cut(s) 52, 490
BciT130I CCWGG 3 cut(s) 88, 97, 426
BciVI GTATCC 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 5
BfuAI ACCTGC 1 cut(s) 352
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 4 cut(s) 171, 224, 227, 338
BlsI GCNGC 4 cut(s) 172, 225, 228, 339
Bme1390I CCNGG 3 cut(s) 88, 97, 426
BmeT110I CYCGRG 2 cut(s) 203, 676
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 2 cut(s) 85, 330
BmrFI CCNGG 3 cut(s) 88, 97, 426
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 3 cut(s) 237, 323, 581
BmuI ACTGGG 1 cut(s) 379
BpmI CTGGAG 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 429
BsaBI GATNNNNATC 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 469
BseBI CCWGG 3 cut(s) 88, 97, 426
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 3 cut(s) 92, 104, 520
BseJI GATNNNNATC 1 cut(s) 469
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 2 cut(s) 166, 169
BseXI GCAGC 3 cut(s) 235, 238, 349
BseYI CCCAGC 1 cut(s) 302
BshFI GGCC 2 cut(s) 295, 329
BsiHKCI CYCGRG 2 cut(s) 203, 676
BsmAI GTCTC 1 cut(s) 5
BsmI GAATGC 2 cut(s) 288, 322
BsnI GGCC 2 cut(s) 295, 329
BsoBI CYCGRG 2 cut(s) 203, 676
Bsp143I GATC 2 cut(s) 560, 680
BspACI CCGC 1 cut(s) 171
BspANI GGCC 2 cut(s) 295, 329
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 85, 330
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 2 cut(s) 568, 688
BspQI GCTCTTC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 1 cut(s) 385
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 2 cut(s) 560, 680
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 3 cut(s) 88, 97, 426
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 370
BstC8I GCNNGC 2 cut(s) 73, 231
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 3 cut(s) 92, 104, 520
BstHHI GCGC 3 cut(s) 349, 434, 655
BstKTI GATC 2 cut(s) 563, 683
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 2 cut(s) 560, 680
BstMWI GCNNNNNNNGC 3 cut(s) 346, 370, 379
BstNI CCWGG 3 cut(s) 88, 97, 426
BstSCI CCNGG 3 cut(s) 86, 95, 424
BstV1I GCAGC 3 cut(s) 235, 238, 349
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 2 cut(s) 295, 329
BtsCI GGATG 3 cut(s) 92, 104, 520
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 2 cut(s) 73, 231
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 3 cut(s) 349, 434, 655
Cfr13I GGNCC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 26
CviAII CATG 3 cut(s) 41, 361, 539
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 562, 682
DpnII GATC 2 cut(s) 560, 680
EaeI YGGCCR 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 123
Eco57I CTGAAG 2 cut(s) 63, 293
Eco88I CYCGRG 2 cut(s) 203, 676
EcoRII CCWGG 3 cut(s) 86, 95, 424
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 3 cut(s) 44, 364, 542
FatI CATG 3 cut(s) 40, 360, 538
FauNDI CATATG 2 cut(s) 67, 369
Fnu4HI GCNGC 4 cut(s) 171, 224, 227, 338
FokI GGATG 3 cut(s) 79, 91, 527
Fsp4HI GCNGC 4 cut(s) 171, 224, 227, 338
GlaI GCGC 3 cut(s) 348, 433, 654
GluI GCNGC 4 cut(s) 171, 224, 227, 338
GsaI CCCAGC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 2 cut(s) 295, 329
HhaI GCGC 3 cut(s) 349, 434, 655
Hin1II CATG 3 cut(s) 44, 364, 542
Hin6I GCGC 3 cut(s) 347, 432, 653
HinP1I GCGC 3 cut(s) 347, 432, 653
HinfI GANTC 2 cut(s) 238, 256
HphI GGTGA 1 cut(s) 58
Hpy188III TCNNGA 2 cut(s) 205, 678
HpyAV CCTTC 6 cut(s) 317, 545, 575, 605, 635, 665
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 4 cut(s) 71, 116, 337, 364
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 370, 379
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 3 cut(s) 44, 364, 542
HspAI GCGC 3 cut(s) 347, 432, 653
Kzo9I GATC 2 cut(s) 560, 680
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 569, 650
Lsp1109I GCAGC 3 cut(s) 235, 238, 349
LweI GCATC 3 cut(s) 237, 323, 581
MalI GATC 2 cut(s) 562, 682
MboI GATC 2 cut(s) 560, 680
MboII GAAGA 2 cut(s) 69, 176
MflI RGATCY 1 cut(s) 680
MluCI AATT 1 cut(s) 146
MseI TTAA 1 cut(s) 198
MspR9I CCNGG 3 cut(s) 88, 97, 426
Mva1269I GAATGC 2 cut(s) 288, 322
MvaI CCWGG 3 cut(s) 88, 97, 426
MwoI GCNNNNNNNGC 3 cut(s) 346, 370, 379
NdeI CATATG 2 cut(s) 67, 369
NdeII GATC 2 cut(s) 560, 680
NlaIII CATG 3 cut(s) 44, 364, 542
NlaIV GGNNCC 2 cut(s) 85, 330
PaeR7I CTCGAG 1 cut(s) 203
PaqCI CACCTGC 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 82
PctI GAATGC 2 cut(s) 288, 322
PfeI GAWTC 2 cut(s) 238, 256
PfoI TCCNGGA 1 cut(s) 95
PkrI GCNGC 4 cut(s) 172, 225, 228, 339
Psp6I CCWGG 3 cut(s) 86, 95, 424
PspFI CCCAGC 1 cut(s) 302
PspGI CCWGG 3 cut(s) 86, 95, 424
PspN4I GGNNCC 2 cut(s) 85, 330
PspPI GGNCC 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 1 cut(s) 680
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 4 cut(s) 171, 224, 227, 338
Sau3AI GATC 2 cut(s) 560, 680
Sau96I GGNCC 1 cut(s) 328
ScrFI CCNGG 3 cut(s) 88, 97, 426
SfaNI GCATC 3 cut(s) 237, 323, 581
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 2 cut(s) 203, 444
SmoI CTYRAG 2 cut(s) 203, 444
Sse9I AATT 1 cut(s) 146
SsiI CCGC 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 86, 95, 424
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 204, 406
TasI AATT 1 cut(s) 146
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 2 cut(s) 238, 256
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseI GCWGC 3 cut(s) 223, 226, 337
TspDTI ATGAA 4 cut(s) 93, 159, 251, 269
XhoI CTCGAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.