MD03G1034200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001007
Physical Location & Seq
Forward (+)
2726178 .. 2728205
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1034200.v1.1.491

Sequence Viewer

Length: 378 bp
ATGGTAACGGTTCAACTTGAGCAGTCAAAGACCTCACAACAGCAGGCGATGAACCAGCATATCATGATGCAGCAACACCAAATATTAATGTTGCTTATTCCACAAATGCAACGGCCACCACTCAGCCCTCAAGGATCACAACATTCGGCCCCTAGTCAGCCTGTGCCTTCATATCCCATGGGTCAGATGCCTTTTCAGTCGCAGTTTCCAATGCCAATATATCGACCTCAGATGGCTTCTTCCGGTGACTCACATATTTCTGCAGGTGTTTTCAATGGTAAATTATCCTGGGGTTTGTTTTCCAGATTGTTGTTGGGTAGTTTTGATGGGGATGTGATGAGGTATTTGGGTGCACATGGCTCGGGTTCTATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

13.83

Weight (kDa)

8.23

Isoelectric Point (pI)

68.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 254
AclWI GGATC 1 cut(s) 142
AcoI YGGCCR 1 cut(s) 113
AgsI TTSAA 3 cut(s) 14, 274, 374
AjnI CCWGG 1 cut(s) 287
Alw21I GWGCWC 1 cut(s) 355
Alw44I GTGCAC 1 cut(s) 351
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 361
AoxI GGCC 2 cut(s) 113, 147
ApaLI GTGCAC 1 cut(s) 351
ApeKI GCWGC 1 cut(s) 70
AseI ATTAAT 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 257
AvaI CYCGRG 1 cut(s) 361
BaeGI GKGCMC 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 355
BbvI GCAGC 1 cut(s) 82
BccI CCATC 2 cut(s) 226, 320
BceAI ACGGC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BciT130I CCWGG 1 cut(s) 289
BfaI CTAG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 261
BfuAI ACCTGC 1 cut(s) 254
BisI GCNGC 1 cut(s) 71
BlsI GCNGC 1 cut(s) 72
Bme1390I CCNGG 1 cut(s) 289
BmeT110I CYCGRG 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 2 cut(s) 57, 177
BpuEI CTTGAG 2 cut(s) 38, 114
BsaJI CCNNGG 2 cut(s) 177, 288
BsaWI WCCGGW 1 cut(s) 242
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 177, 288
BseGI GGATG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 136, 242
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 1 cut(s) 82
BshFI GGCC 2 cut(s) 115, 149
BsiHKAI GWGCWC 1 cut(s) 355
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 1 cut(s) 243
BsnI GGCC 2 cut(s) 115, 149
BsoBI CYCGRG 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 1 cut(s) 134
Bsp19I CCATGG 1 cut(s) 177
BspANI GGCC 2 cut(s) 115, 149
BspCNI CTCAG 2 cut(s) 135, 241
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 150
BspMAI CTGCAG 1 cut(s) 265
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 177, 288
BssMI GATC 1 cut(s) 134
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 2 cut(s) 122, 228
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 337
BstKTI GATC 1 cut(s) 137
BstMBI GATC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 287
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 82
BsuRI GGCC 2 cut(s) 115, 149
BtgI CCRYGG 1 cut(s) 177
BtgZI GCGATG 1 cut(s) 62
BtsCI GGATG 1 cut(s) 337
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 1 cut(s) 45
CciI TCATGA 1 cut(s) 63
Cfr13I GGNCC 1 cut(s) 148
CviAII CATG 3 cut(s) 64, 178, 356
CviJI RGCY 6 cut(s) 115, 126, 149, 160, 236, 360
CviKI_1 RGCY 6 cut(s) 115, 126, 149, 160, 236, 360
DdeI CTNAG 2 cut(s) 122, 228
DpnI GATC 1 cut(s) 136
DpnII GATC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 3 cut(s) 67, 181, 359
FaiI YATR 7 cut(s) 60, 65, 172, 179, 220, 255, 357
FatI CATG 3 cut(s) 63, 177, 355
Fnu4HI GCNGC 1 cut(s) 71
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 71
FspBI CTAG 1 cut(s) 153
GluI GCNGC 1 cut(s) 71
HaeIII GGCC 2 cut(s) 115, 149
HapII CCGG 1 cut(s) 243
Hin1II CATG 3 cut(s) 67, 181, 359
HinfI GANTC 1 cut(s) 248
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 1 cut(s) 353
Hpy188I TCNGA 2 cut(s) 186, 231
Hpy188III TCNNGA 2 cut(s) 64, 303
Hpy8I GTNNAC 1 cut(s) 353
HpyAV CCTTC 1 cut(s) 177
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 4 cut(s) 70, 109, 263, 353
HpyF3I CTNAG 2 cut(s) 122, 228
Hsp92II CATG 3 cut(s) 67, 181, 359
Kzo9I GATC 1 cut(s) 134
LpnPI CCDG 8 cut(s) 29, 68, 174, 249, 256, 274, 301, 316
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 2 cut(s) 57, 177
MaeI CTAG 1 cut(s) 153
MaeIII GTNAC 2 cut(s) 4, 245
MalI GATC 1 cut(s) 136
MboI GATC 1 cut(s) 134
MboII GAAGA 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 1 cut(s) 281
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 4 cut(s) 43, 138, 237, 333
MseI TTAA 1 cut(s) 86
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 289
MvaI CCWGG 1 cut(s) 289
NcoI CCATGG 1 cut(s) 177
NdeII GATC 1 cut(s) 134
NlaIII CATG 3 cut(s) 67, 181, 359
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 1 cut(s) 245
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 1 cut(s) 254
PkrI GCNGC 1 cut(s) 72
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PshBI ATTAAT 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 148
PstI CTGCAG 1 cut(s) 265
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 1 cut(s) 71
Sau3AI GATC 1 cut(s) 134
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 1 cut(s) 355
SetI ASST 4 cut(s) 35, 229, 268, 344
SfaNI GCATC 2 cut(s) 57, 177
SfcI CTRYAG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 17, 129
SmoI CTYRAG 2 cut(s) 17, 129
Sse9I AATT 1 cut(s) 281
SspI AATATT 1 cut(s) 84
SspMI CTAG 1 cut(s) 153
StyD4I CCNGG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 1 cut(s) 223
TasI AATT 1 cut(s) 281
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 65, 159
VneI GTGCAC 1 cut(s) 351
VspI ATTAAT 1 cut(s) 86
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.