MD03G1145300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001007
Physical Location & Seq
Forward (+)
15680104 .. 15680722
619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1145300.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGTAGCCTCAGAGGTTAAGGATCCAAGACATATTCCACTGAAGAAGGATTGGCGTGGGGTTCCTGCAAATGCTAAGGAACTTATGTGGGAAAGAATTAAGGGTAATATCTTGTTTCTTGATGAAGATCAAGATAGAATGCCAATTATTTGCAACATGACTCTCCATGTTGCGGATCATGCATACAAGGAATATCGTAATTGGTTGAAAGCAGAGTATTACACCAAAAGGCAAGAGCATGAGCGCTCAGAACCTCCCCCTGGTGTGGATGGTGGTCAGTGGGCCGAGATGTTAGCGTATTGGGATGATCCTAAAACAAAGGAGGTGGCAGAGAAAAACAAGATTAATCGTGTGTCAAAAAAGATGAACCATACTACTGGGTCAAAAACGTTTGCTCGGGTTCGTGGGGAGCTTGTAAGTGTTGCTATATTCTTTTATTTGAAGTGTTTTAATTTTTTTTATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

156

Amino Acids

18.34

Weight (kDa)

8.98

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 93 - 138 4.2e-11 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 466
AciI CCGC 1 cut(s) 173
AclI AACGTT 1 cut(s) 389
AclWI GGATC 4 cut(s) 17, 30, 183, 302
AcuI CTGAAG 1 cut(s) 62
AfeI AGCGCT 1 cut(s) 245
AfiI CCNNNNNNNGG 3 cut(s) 172, 260, 265
AgsI TTSAA 2 cut(s) 208, 442
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 1 cut(s) 412
AluI AGCT 1 cut(s) 412
AlwI GGATC 4 cut(s) 17, 30, 183, 302
Ama87I CYCGRG 1 cut(s) 396
Aor51HI AGCGCT 1 cut(s) 245
AoxI GGCC 1 cut(s) 282
AseI ATTAAT 1 cut(s) 345
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 282
AvaI CYCGRG 1 cut(s) 396
BamHI GGATCC 1 cut(s) 22
BccI CCATC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 261
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 24, 63
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 387
BmuI ACTGGG 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 259
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 260, 265
Bse1I ACTGG 1 cut(s) 382
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 2 cut(s) 274, 310
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 3 cut(s) 172, 260, 265
BseMII CTCAG 2 cut(s) 24, 261
BseNI ACTGG 1 cut(s) 382
BshFI GGCC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 396
BslI CCNNNNNNNGG 3 cut(s) 172, 260, 265
BsmI GAATGC 1 cut(s) 144
BsnI GGCC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 396
Bsp143I GATC 4 cut(s) 22, 127, 175, 307
BspACI CCGC 1 cut(s) 173
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 2 cut(s) 23, 260
BspLI GGNNCC 2 cut(s) 24, 63
BspPI GGATC 4 cut(s) 17, 30, 183, 302
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 4 cut(s) 22, 127, 175, 307
Bst2UI CCWGG 1 cut(s) 261
BstDEI CTNAG 3 cut(s) 10, 75, 247
BstF5I GGATG 2 cut(s) 274, 310
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 4 cut(s) 25, 130, 178, 310
BstMBI GATC 4 cut(s) 22, 127, 175, 307
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstX2I RGATCY 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 377
BstYI RGATCY 1 cut(s) 22
BsuRI GGCC 1 cut(s) 284
BtsCI GGATG 2 cut(s) 274, 310
BtsIMutI CAGTG 2 cut(s) 38, 284
CfoI GCGC 1 cut(s) 246
Cfr13I GGNCC 1 cut(s) 282
CspCI CAANNNNNGTGG 2 cut(s) 306, 341
CviAII CATG 4 cut(s) 157, 167, 179, 239
CviJI RGCY 3 cut(s) 8, 284, 412
CviKI_1 RGCY 3 cut(s) 8, 284, 412
DdeI CTNAG 3 cut(s) 10, 75, 247
DpnI GATC 4 cut(s) 24, 129, 177, 309
DpnII GATC 4 cut(s) 22, 127, 175, 307
Eco47III AGCGCT 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 62
Eco88I CYCGRG 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 259
EcoT22I ATGCAT 1 cut(s) 184
FaeI CATG 4 cut(s) 160, 170, 182, 242
FatI CATG 4 cut(s) 156, 166, 178, 238
FokI GGATG 2 cut(s) 281, 317
GlaI GCGC 1 cut(s) 245
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 1 cut(s) 284
HhaI GCGC 1 cut(s) 246
Hin1II CATG 4 cut(s) 160, 170, 182, 242
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HinfI GANTC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 13, 250
Hpy188III TCNNGA 2 cut(s) 119, 131
HpyAV CCTTC 1 cut(s) 40
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 3 cut(s) 68, 153, 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 3 cut(s) 10, 75, 247
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 4 cut(s) 160, 170, 182, 242
HspAI GCGC 1 cut(s) 244
Kzo9I GATC 4 cut(s) 22, 127, 175, 307
LmnI GCTCC 1 cut(s) 409
LpnPI CCDG 4 cut(s) 78, 246, 273, 363
MaeII ACGT 1 cut(s) 389
MalI GATC 4 cut(s) 24, 129, 177, 309
MboI GATC 4 cut(s) 22, 127, 175, 307
MboII GAAGA 2 cut(s) 55, 137
MflI RGATCY 1 cut(s) 22
MluCI AATT 4 cut(s) 96, 144, 199, 451
MlyI GAGTC 1 cut(s) 154
MnlI CCTC 4 cut(s) 7, 19, 264, 316
Mph1103I ATGCAT 1 cut(s) 184
MseI TTAA 4 cut(s) 18, 99, 345, 450
MspR9I CCNGG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 144
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 4 cut(s) 22, 127, 175, 307
NlaIII CATG 4 cut(s) 160, 170, 182, 242
NlaIV GGNNCC 2 cut(s) 24, 63
NmeAIII GCCGAG 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 184
PctI GAATGC 1 cut(s) 144
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 345
PsiI TTATAA 1 cut(s) 466
Psp1406I AACGTT 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 24, 63
PspPI GGNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 22
SaqAI TTAA 4 cut(s) 18, 99, 345, 450
Sau3AI GATC 4 cut(s) 22, 127, 175, 307
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 1 cut(s) 261
SetI ASST 5 cut(s) 18, 256, 327, 392, 414
Sse9I AATT 4 cut(s) 96, 144, 199, 451
SsiI CCGC 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 259
TaiI ACGT 1 cut(s) 392
TasI AATT 4 cut(s) 96, 144, 199, 451
Tru1I TTAA 4 cut(s) 18, 99, 345, 450
Tru9I TTAA 4 cut(s) 18, 99, 345, 450
TscAI CASTG 2 cut(s) 45, 284
TspDTI ATGAA 2 cut(s) 138, 380
TspRI CASTG 2 cut(s) 45, 284
VspI ATTAAT 1 cut(s) 345
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.