pycom04g18660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001007
Physical Location & Seq
Reverse (-)
20619310 .. 20621104
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18660.2

Sequence Viewer

Length: 1221 bp
ATGGATAGTTCTCGTGAAGATCAACGTTCTGAAGAACGTCACTTGGAAGAGGACGAGGGTGATGCTGGTACATCTCAACGGAGGCGTGGACCCTCAATTCCTAAATGGACGAAACAGCTTTGTTCGTTTGATTCGTCCGGAAAATGCATTAGTGATAATGCTAGTGCTTTTTCAAAACATGTAGCAGCGGAGGTTAGGGACTATAGAAATCTGCCGTTGGAGAAAGATTGGCGTAAGGTTACGGAAGAAGATAAGGAAGCTTTCTGGAATAGAATAAAGGGAAATATTTTTTTTGAAGATGCAGATATAGTAAAAATGCCTTTGATCCGTCATATGACGCTTAAGATTGCAGAGCATGCGCACAAAGAGTTTAGAAATAAGTTAAAAAAAAAGTATTATACTAAAAGAGCAGAAGAGGTGCGCAGCGAGCCTCCTCCTGATGTGATTCCTGCCCAGTGGGATGATTTGTTGGCATATTGGAATAGAGACAAAACAAAGGAGGTTGCTGAAAAAAATAAGATTAATCGGGGATTAAAATCAATGAACCATACTACTGGTACAAAATCGTTTGCAAGAGTGCGGGAAGAATATCGGAAGAAGCATGGGAAAGAAGCAGATCCTGTAACCTTTTTTCGTGAATGTCATACACGAAAAGATAAAACATGGATTGATGAAACATCGGAGCATACAGGGTCAACAATGGAGAGCAACCTGCAAACATTGATTGAGTCGGGCCAAGACGATAATGAAGATCTTAGGACCCAAGTTTATGTTGATACTATGGGTCCTGAGAGGTATAATAGAGTCAGAGGATACGGTCATGGTGTGACTCCTGATATGGTGTCTTATGCATCTTCTTCATCTTCTACATCAATTTCCTCCAGGAGATCATCTAAGAGTTCAATGGCGTTGCTGATGACTCAAAATAATGAGTTGAAAATGAGGGACGAAAATAATAATAAACGGATTTCGGAACTAGAGAACAAGCAAAATCAGTTGTTTGCATGGTTTTTTCAAAGATTTCAGCCACAACCACCGTATAGCCCGGGAACCCAACAATCAGGCCACAGTCAACCTCCTCAACAGTCGGGCCAAAGTCAGCCTCCCCCTGCATATCCGTATGCTGGCCATAATCAGCAACCTCCATATCCTGTGTATCTGATGCCTGCGCAGCCCATCCGCCCATGCCATACATGCAGCAGCCACTTTGTCAAGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

407

Amino Acids

47.03

Weight (kDa)

8.8

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 360, 422, 1170
Acc36I ACCTGC 1 cut(s) 720
AccIII TCCGGA 1 cut(s) 137
AciI CCGC 3 cut(s) 188, 580, 1180
AclI AACGTT 1 cut(s) 25
AclWI GGATC 2 cut(s) 319, 611
AcoI YGGCCR 1 cut(s) 1126
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 2 cut(s) 70, 559
AfiI CCNNNNNNNGG 1 cut(s) 1124
AflII CTTAAG 1 cut(s) 341
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 5 cut(s) 174, 296, 903, 937, 1016
AjnI CCWGG 1 cut(s) 881
AluBI AGCT 2 cut(s) 118, 260
AluI AGCT 2 cut(s) 118, 260
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 2 cut(s) 319, 611
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 1045
Aor13HI TCCGGA 1 cut(s) 137
AoxI GGCC 4 cut(s) 733, 1063, 1090, 1126
ApeKI GCWGC 5 cut(s) 185, 423, 1171, 1197, 1200
AseI ATTAAT 1 cut(s) 522
AspLEI GCGC 3 cut(s) 361, 423, 1171
AspS9I GGNCC 5 cut(s) 89, 733, 759, 785, 1090
AsuC2I CCSGG 2 cut(s) 1046, 1047
AsuHPI GGTGA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 1045
AvaII GGWCC 3 cut(s) 89, 759, 785
BalI TGGCCA 1 cut(s) 1128
BauI CACGAG 1 cut(s) 12
BbvI GCAGC 5 cut(s) 197, 435, 1183, 1209, 1212
BccI CCATC 1 cut(s) 1184
BceAI ACGGC 1 cut(s) 199
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BciT130I CCWGG 1 cut(s) 883
BciVI GTATCC 1 cut(s) 806
BcnI CCSGG 2 cut(s) 1046, 1047
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 3 cut(s) 162, 977, 1219
BfmI CTRYAG 1 cut(s) 202
BfrI CTTAAG 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 720
BfuI GTATCC 1 cut(s) 806
BglII AGATCT 1 cut(s) 751
BisI GCNGC 5 cut(s) 186, 424, 1172, 1198, 1201
BlsI GCNGC 5 cut(s) 187, 425, 1173, 1199, 1202
Bme1390I CCNGG 3 cut(s) 883, 1046, 1047
Bme18I GGWCC 3 cut(s) 89, 759, 785
BmeT110I CYCGRG 1 cut(s) 1045
BmgT120I GGNCC 5 cut(s) 89, 733, 759, 785, 1090
BmiI GGNNCC 4 cut(s) 91, 761, 786, 1051
BmrFI CCNGG 3 cut(s) 883, 1046, 1047
BmrI ACTGGG 1 cut(s) 448
BmsI GCATC 4 cut(s) 52, 289, 860, 1152
BmuI ACTGGG 1 cut(s) 448
BpmI CTGGAG 1 cut(s) 865
BpuMI CCSGG 2 cut(s) 1046, 1047
BsaBI GATNNNNATC 1 cut(s) 535
BsaJI CCNNGG 1 cut(s) 1045
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 1 cut(s) 1124
Bse1I ACTGG 2 cut(s) 454, 559
Bse8I GATNNNNATC 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 137
BseBI CCWGG 1 cut(s) 883
BseDI CCNNGG 1 cut(s) 1045
BseGI GGATG 2 cut(s) 466, 1176
BseJI GATNNNNATC 1 cut(s) 535
BseLI CCNNNNNNNGG 1 cut(s) 1124
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 2 cut(s) 454, 559
BseRI GAGGAG 2 cut(s) 423, 1068
BseXI GCAGC 5 cut(s) 197, 435, 1183, 1209, 1212
BshFI GGCC 4 cut(s) 735, 1065, 1092, 1128
BsiHKCI CYCGRG 1 cut(s) 1045
BsiSI CCGG 2 cut(s) 138, 1046
BslFI GGGAC 2 cut(s) 212, 959
BslI CCNNNNNNNGG 1 cut(s) 1124
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 2 cut(s) 212, 959
BsnI GGCC 4 cut(s) 735, 1065, 1092, 1128
BsoBI CYCGRG 1 cut(s) 1045
Bsp13I TCCGGA 1 cut(s) 137
Bsp143I GATC 5 cut(s) 19, 324, 616, 751, 887
BspACI CCGC 3 cut(s) 188, 580, 1180
BspANI GGCC 4 cut(s) 735, 1065, 1092, 1128
BspCNI CTCAG 1 cut(s) 781
BspEI TCCGGA 1 cut(s) 137
BspLI GGNNCC 4 cut(s) 91, 761, 786, 1051
BspMI ACCTGC 1 cut(s) 720
BspPI GGATC 2 cut(s) 319, 611
BspTI CTTAAG 1 cut(s) 341
BsrI ACTGG 2 cut(s) 454, 559
BssECI CCNNGG 1 cut(s) 1045
BssMI GATC 5 cut(s) 19, 324, 616, 751, 887
BssSI CACGAG 1 cut(s) 12
Bst2BI CACGAG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 883
Bst4CI ACNGT 4 cut(s) 818, 1038, 1070, 1086
Bst6I CTCTTC 2 cut(s) 42, 408
BstAFI CTTAAG 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 4 cut(s) 357, 428, 1126, 1167
BstDEI CTNAG 3 cut(s) 755, 789, 894
BstF5I GGATG 2 cut(s) 466, 1176
BstHHI GCGC 3 cut(s) 361, 423, 1171
BstKTI GATC 5 cut(s) 22, 327, 619, 754, 890
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 5 cut(s) 19, 324, 616, 751, 887
BstMWI GCNNNNNNNGC 4 cut(s) 356, 427, 1171, 1194
BstNI CCWGG 1 cut(s) 883
BstNSI RCATGY 3 cut(s) 182, 359, 1197
BstSCI CCNGG 3 cut(s) 881, 1044, 1045
BstSFI CTRYAG 1 cut(s) 202
BstV1I GCAGC 5 cut(s) 197, 435, 1183, 1209, 1212
BstX2I RGATCY 2 cut(s) 616, 751
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 2 cut(s) 616, 751
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 4 cut(s) 735, 1065, 1092, 1128
BtsCI GGATG 2 cut(s) 466, 1176
BtsIMutI CAGTG 1 cut(s) 461
BveI ACCTGC 1 cut(s) 720
Cac8I GCNNGC 4 cut(s) 357, 428, 1126, 1167
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 3 cut(s) 361, 423, 1171
Cfr13I GGNCC 5 cut(s) 89, 733, 759, 785, 1090
Cfr9I CCCGGG 1 cut(s) 1045
CseI GACGC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 69, 558
CviAII CATG 8 cut(s) 179, 356, 602, 663, 821, 1005, 1185, 1194
CviQI GTAC 2 cut(s) 69, 558
DdeI CTNAG 3 cut(s) 755, 789, 894
DpnI GATC 5 cut(s) 21, 326, 618, 753, 889
DpnII GATC 5 cut(s) 19, 324, 616, 751, 887
EaeI YGGCCR 1 cut(s) 1126
Eam1104I CTCTTC 2 cut(s) 42, 408
EarI CTCTTC 2 cut(s) 42, 408
EciI GGCGGA 1 cut(s) 1169
Eco47I GGWCC 3 cut(s) 89, 759, 785
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 1045
EcoO109I RGGNCCY 2 cut(s) 759, 785
EcoRII CCWGG 1 cut(s) 881
EcoT22I ATGCAT 2 cut(s) 149, 853
FaeI CATG 8 cut(s) 182, 359, 605, 666, 824, 1008, 1188, 1197
FaqI GGGAC 2 cut(s) 212, 959
FatI CATG 8 cut(s) 178, 355, 601, 662, 820, 1004, 1184, 1193
FauI CCCGC 1 cut(s) 573
FauNDI CATATG 1 cut(s) 333
Fnu4HI GCNGC 5 cut(s) 186, 424, 1172, 1198, 1201
FokI GGATG 2 cut(s) 473, 1163
Fsp4HI GCNGC 5 cut(s) 186, 424, 1172, 1198, 1201
FspAI RTGCGCAY 1 cut(s) 360
FspBI CTAG 3 cut(s) 162, 977, 1219
FspI TGCGCA 3 cut(s) 360, 422, 1170
GlaI GCGC 3 cut(s) 360, 422, 1170
GluI GCNGC 5 cut(s) 186, 424, 1172, 1198, 1201
GsuI CTGGAG 1 cut(s) 865
HaeIII GGCC 4 cut(s) 735, 1065, 1092, 1128
HapII CCGG 2 cut(s) 138, 1046
HgaI GACGC 1 cut(s) 346
HhaI GCGC 3 cut(s) 361, 423, 1171
Hin1II CATG 8 cut(s) 182, 359, 605, 666, 824, 1008, 1188, 1197
Hin6I GCGC 3 cut(s) 359, 421, 1169
HinP1I GCGC 3 cut(s) 359, 421, 1169
HincII GTYRAC 2 cut(s) 696, 1073
HindII GTYRAC 2 cut(s) 696, 1073
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 6 cut(s) 131, 445, 728, 804, 829, 919
HpaII CCGG 2 cut(s) 138, 1046
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 3 cut(s) 89, 696, 1073
Hpy188I TCNGA 6 cut(s) 31, 594, 682, 809, 973, 1161
Hpy188III TCNNGA 7 cut(s) 14, 138, 265, 437, 635, 788, 833
Hpy8I GTNNAC 3 cut(s) 89, 696, 1073
HpyCH4III ACNGT 4 cut(s) 818, 1038, 1070, 1086
HpyCH4IV ACGT 2 cut(s) 25, 37
HpyCH4V TGCA 9 cut(s) 147, 302, 350, 572, 715, 851, 1004, 1112, 1197
HpyF10VI GCNNNNNNNGC 4 cut(s) 356, 427, 1171, 1194
HpyF3I CTNAG 3 cut(s) 755, 789, 894
HpySE526I ACGT 2 cut(s) 25, 37
Hsp92II CATG 8 cut(s) 182, 359, 605, 666, 824, 1008, 1188, 1197
HspAI GCGC 3 cut(s) 359, 421, 1169
Kpn2I TCCGGA 1 cut(s) 137
Kzo9I GATC 5 cut(s) 19, 324, 616, 751, 887
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 5 cut(s) 197, 435, 1183, 1209, 1212
LweI GCATC 4 cut(s) 52, 289, 860, 1152
MaeI CTAG 3 cut(s) 162, 977, 1219
MaeII ACGT 2 cut(s) 25, 37
MaeIII GTNAC 4 cut(s) 38, 238, 622, 826
MalI GATC 5 cut(s) 21, 326, 618, 753, 889
MboI GATC 5 cut(s) 19, 324, 616, 751, 887
MflI RGATCY 2 cut(s) 616, 751
MlsI TGGCCA 1 cut(s) 1128
MluCI AATT 2 cut(s) 96, 873
MluNI TGGCCA 1 cut(s) 1128
MlyI GAGTC 4 cut(s) 737, 813, 823, 913
MmeI TCCRAC 1 cut(s) 198
Mox20I TGGCCA 1 cut(s) 1128
Mph1103I ATGCAT 2 cut(s) 149, 853
MroI TCCGGA 1 cut(s) 137
MscI TGGCCA 1 cut(s) 1128
MseI TTAA 4 cut(s) 342, 383, 522, 533
Msp20I TGGCCA 1 cut(s) 1128
MspA1I CMGCKG 1 cut(s) 188
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 2 cut(s) 138, 1046
MspR9I CCNGG 3 cut(s) 883, 1046, 1047
MvaI CCWGG 1 cut(s) 883
MwoI GCNNNNNNNGC 4 cut(s) 356, 427, 1171, 1194
NciI CCSGG 2 cut(s) 1046, 1047
NdeI CATATG 1 cut(s) 333
NdeII GATC 5 cut(s) 19, 324, 616, 751, 887
NlaIII CATG 8 cut(s) 182, 359, 605, 666, 824, 1008, 1188, 1197
NlaIV GGNNCC 4 cut(s) 91, 761, 786, 1051
NmuCI GTSAC 2 cut(s) 38, 826
NsbI TGCGCA 3 cut(s) 360, 422, 1170
NsiI ATGCAT 2 cut(s) 149, 853
NspI RCATGY 3 cut(s) 182, 359, 1197
PaeI GCATGC 1 cut(s) 359
PciI ACATGT 1 cut(s) 178
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PfeI GAWTC 2 cut(s) 131, 445
PfoI TCCNGGA 1 cut(s) 881
PkrI GCNGC 5 cut(s) 187, 425, 1173, 1199, 1202
PleI GAGTC 4 cut(s) 736, 812, 823, 913
PpsI GAGTC 4 cut(s) 736, 812, 823, 913
PpuMI RGGWCCY 2 cut(s) 759, 785
PscI ACATGT 1 cut(s) 178
PshBI ATTAAT 1 cut(s) 522
Psp1406I AACGTT 1 cut(s) 25
Psp5II RGGWCCY 2 cut(s) 759, 785
Psp6I CCWGG 1 cut(s) 881
PspGI CCWGG 1 cut(s) 881
PspN4I GGNNCC 4 cut(s) 91, 761, 786, 1051
PspPI GGNCC 5 cut(s) 89, 733, 759, 785, 1090
PspPPI RGGWCCY 2 cut(s) 759, 785
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 2 cut(s) 616, 751
RsaI GTAC 2 cut(s) 70, 559
RsaNI GTAC 2 cut(s) 69, 558
SaqAI TTAA 4 cut(s) 342, 383, 522, 533
SatI GCNGC 5 cut(s) 186, 424, 1172, 1198, 1201
Sau3AI GATC 5 cut(s) 19, 324, 616, 751, 887
Sau96I GGNCC 5 cut(s) 89, 733, 759, 785, 1090
SchI GAGTC 4 cut(s) 737, 813, 823, 913
ScrFI CCNGG 3 cut(s) 883, 1046, 1047
SfaNI GCATC 4 cut(s) 52, 289, 860, 1152
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 3 cut(s) 89, 759, 785
SmaI CCCGGG 1 cut(s) 1047
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SphI GCATGC 1 cut(s) 359
Sse9I AATT 2 cut(s) 96, 873
SsiI CCGC 3 cut(s) 188, 580, 1180
SspI AATATT 1 cut(s) 286
SspMI CTAG 3 cut(s) 162, 977, 1219
StyD4I CCNGG 3 cut(s) 881, 1044, 1045
TaaI ACNGT 4 cut(s) 818, 1038, 1070, 1086
TaiI ACGT 2 cut(s) 28, 40
TasI AATT 2 cut(s) 96, 873
TfiI GAWTC 2 cut(s) 131, 445
Tru1I TTAA 4 cut(s) 342, 383, 522, 533
Tru9I TTAA 4 cut(s) 342, 383, 522, 533
TscAI CASTG 1 cut(s) 461
TseFI GTSAC 2 cut(s) 38, 826
TseI GCWGC 5 cut(s) 185, 423, 1171, 1197, 1200
Tsp45I GTSAC 2 cut(s) 38, 826
TspDTI ATGAA 4 cut(s) 557, 687, 762, 849
TspGWI ACGGA 5 cut(s) 94, 257, 317, 979, 1107
TspMI CCCGGG 1 cut(s) 1045
TspRI CASTG 1 cut(s) 461
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 3 cut(s) 89, 759, 785
VspI ATTAAT 1 cut(s) 522
XceI RCATGY 3 cut(s) 182, 359, 1197
XmaI CCCGGG 1 cut(s) 1045
XspI CTAG 3 cut(s) 162, 977, 1219
Zsp2I ATGCAT 2 cut(s) 149, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.