Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000403

Physical Location & Seq

Genomic Coordinates Reverse (-)

3091077 .. 3093125

Gene Structure 2049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1047100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGGCAAGCATGCTTTCTTCTCTTGCTTTCGTCATCTGCGTTCTCTGTTTTTTGGCGTCGACTCCGCAGGCCGCCCCTGCTACAGCCCCGTTCCTTTTCCCGGAAGCTCAATCCACCGTCCTCCCCGACCCTTTGACCTTCTTCGCCCCGAACCTCCTCTCATCCCCACTCCCTACCAGCTCATTCTTTCAAAACTTTGTCCTTCACAATGGCGACCAACCGGAGTACATACACCCCTACATCATCAGATCAGCCAACTCTTCCCTGTCTTTCGCTTACCCGTCTTTCTCCTACACCTCTACCGTCGTATCCACATCCCCGTTCGAAGCCGACCTCACCATCTTCGCCTCAAAATCAAACAACAAGGGCCACCATGTAGTCTCCGACTTCAATGATTTGAGTGTGACATTGGACTTTCCCTCCTCCAACCTCCGCTTCTACCTCGTCCGCGGAAGCCCCTTTCTCAGCTGCTTTGTTTATCGCCCCACGGCGGTTTCTTTATCGACTGATCATAAAATTGTCTCGGCAACATCAGCGAGTGGCTCAAATACTAAGTTCATCGTCAAGCTCGACAATGATCAGACATGGCTAGTATACACATCCTCACCAACAGAATTAACTCACAGCGGCTCATCCATGTTAACTCTTCAGGACTTTTCTGGGGTTGTTCGTTTTGCTGTAATGCCGGATCCTGATCCTAAATCTGAGGGAATTCTTGACCACTTCAGTTCTTGTTACCCGGTTTCTGGTGCAGCTGTGCTCAAAAACTCAAATACTTTGTCGTATAAATTTGAAAAGATAGGCGGAGACTTGCTCATGCTAGCTCATCCTCTACATCTCAAGCTTCTGTCCAATGCCACAGTTTTGCAGGATTTTAAGTACAAAAGCATTGACGGGGATTTGATTGGCGTTGTTGGTGATTCGTGGGTGTTAGCACCAAAGCCTATTCCAGTCACTTGGAATTCCGTTGGAGGTGTCAAAAAAGATTCATTCCCTGAAATCATATCTGCGCTTCGTCGAGACGTTGACGCTCTTAGTTCTACACAATCAACTACTTCGTCTTCATATTTTTATGGGAAACTGGTTGCTAGAGCGGCAAGGCTGGCTCTGATCGCCGAGGAGGTAGCTTTCCCTGATGTGATACCCGTGATTAGGACATTCTTAAGGGAGAAAATTGAGCCGTGGTTGGATGGTACTTTTAGCGGCAACGGATTTTTGCACGATAAAAAATGGGGTGGACTTGTTACGAAATCCTCCACAGCTGATCCAGGTGCTGATTTTGGGTTTGGTCTTTATTCCGATCACCATTTTCATCTGGGGTACTTTATATATGGAATTTCCGTGTTAGCAAAGATTGATCCTGCATGGGGGGAGAAGTACAAGCCTCAAGCTTACTCGCTCGTGGAGGATTTTCTCAACAAGGACAAGCAATCGAATCCAAATTATCCGCGTCTGAGAACCTTTGATCTTTATAAATTGCACTCGTGGGCGGGAGGGTTGACAGAATTCGCAAACGGGAGGAATCAAGAGAGCACGAGTGAGGCGGTTAATGCTTACTATGCAGCTTCTCTGATGGGAAAAGCCTATAACGACGCGCAACTTGAGGCCACCGGTTCACTGCTTACTACATTGGAAATTCAGGCAGCTCAAATGTGGTGGCACGTGAGAGAGGGAGGAGAGAGTCAAATTTACCAGCAAGATTTCGTGAAGGAAAACCGAGTTGTGGGAATTTTATGGGCGACCAAGAGAGACAGCGGGCTTTGGTTTGCGACTGCGGAGGCAAGAGAAATCAGACTTGGAATCCAAGTACTGCCTGTTTTGCCAGTTACTGAGGTATTGTTCTCGGACGCGGGTTTCGCCAGTGAGCTAGTGAACTGGGCATTACCGGCGCTCAGCAGGACGGGAGTCACGGAAGGATGGAAGGGATTTGTATTTGCATTACAAGGGATGTTCGACAAAGAAGGCGCTTTGGAGAAGATTAGAAGCTTGAAAGAATTCGACGACGGGAACTCCCTCAGTAATCTCTTATGGTGGATTCACAGTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0000746 GO:0000747 GO:0000920 GO:0000935 GO:0000936 GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005937 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006076 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0009986 GO:0009987 GO:0010383 GO:0015629 GO:0015926 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016998 GO:0019953 GO:0022411 GO:0022414 GO:0030246 GO:0030247 GO:0030427 GO:0030428 GO:0030994 GO:0031505 GO:0032153 GO:0032155 GO:0042973 GO:0042995 GO:0043170 GO:0043187 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043332 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044277 GO:0044347 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044457 GO:0044463 GO:0044464 GO:0044703 GO:0044764 GO:0045229 GO:0051273 GO:0051275 GO:0051286 GO:0051301 GO:0051704 GO:0070871 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0071853 GO:0120025 GO:0120038 GO:1901575 GO:1904541 GO:1990819

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_81

Protein Analysis

683

Amino Acids

75.18

Weight (kDa)

5.76

Isoelectric Point (pI)

38.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_81	PF03639	54 - 319	2.1e-52	Glycosyl hydrolase family 81 N-terminal domain
Glyco_hydro81C	PF17652	327 - 678	6.7e-101	Glycosyl hydrolase family 81 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18310 AT5G15870
fragaria_vesca	FvH4_3g44967 FvH4_6g45580 FvH4_6g47860 FvH4_6g47870 FvH4_6g53160
malus_domestica	MD09G1047100.v1.1 MD09G1081400.v1.1 MD09G1081500.v1.1 MD09G1081600.v1.1 MD09G1081700.v1.1 MD15G1128600.v1.1 MD17G1047200.v1.1 MD17G1048700.v1.1 MD17G1071200.v1.1 MD17G1072700.v1.1
prunus_persica	Prupe.3G242000_v2.0.a1 Prupe.3G242100_v2.0.a1 Prupe.3G261700_v2.0.a1 Prupe.8G191700_v2.0.a1 Prupe.8G194600_v2.0.a1 Prupe.8G259500_v2.0.a1
pyrus_communis	pycom09g00810 pycom09g00820 pycom09g00830 pycom15g11570 pycom17g04350 pycom17g07070 pycom17g07350
rosa_chinensis	RchiOBHm_Chr1g0366281 RchiOBHm_Chr2g0163531 RchiOBHm_Chr2g0163581 RchiOBHm_Chr2g0167061 RchiOBHm_Chr2g0167071 RchiOBHm_Chr3g0490821 RchiOBHm_Chr3g0490841 RchiOBHm_Chr3g0492161 RchiOBHm_Chr3g0492171
rosa_laevigata	RLG00000021408 RLG00000021693 RLG00000021694 RLG00000022819 RLG00000022827
rosa_multiflora	Rmu_co8304887.1_g000001 Rmu_co8334235.1_g000001 Rmu_co8488335.1_g000001 Rmu_sc0003484.1_g000001 Rmu_sc0003484.1_g000004 Rmu_sc0010966.1_g000013 Rmu_sc0012462.1_g000001 Rmu_sc0019504.1_g000002 Rmu_sc0019504.1_g000004 Rmu_sc0022540.1_g000001 Rmu_sc0023760.1_g000001 Rmu_sc0038280.1_g000002
rosa_roxburghii	Rroxscaffold_2G00077500 Rroxscaffold_2G00083750 Rroxscaffold_2G00083760 Rroxscaffold_2G00087160 Rroxscaffold_2G00132530 Rroxscaffold_4G00290030 Rroxscaffold_4G00290040 Rroxscaffold_4G00290280
rosa_rugosa	Rorug01G0328400 Rorug02G0265200 Rorug02G0265900 Rorug02G0266000 Rorug02G0266800.1 Rorug02G0266900.1 Rorug02G0267500 Rorug02G0267600 Rorug02G0268300 Rorug02G0268800 Rorug02G0268900 Rorug02G0503600 Rorug02G0528200 Rorug02G0528300 Rorug03G0247200 Rorug03G0247300
rosa_samantha	Rh1BG298400 Rh1DG329800 Rh2AG570500 Rh2AG570700 Rh2AG595000 Rh2AG595100 Rh2AG595200 Rh2CG552500 Rh2CG552700 Rh2CG577100 Rh2CG577200 Rh2DG592500 Rh2DG617800 Rh2DG617900 Rh3CG333000 Rh3DG335000
rosa_wichuraiana	Rw2G047230 Rw2G049530 Rw2G049540 Rw3G026510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1473
AccBSI	CCGCTC	1 cut(s)	1094
AccI	GTMKAC	2 cut(s)	59, 594
AccII	CGCG	4 cut(s)	450, 1450, 1595, 1850
AclWI	GGATC	5 cut(s)	683, 689, 696, 1259, 1352
AcsI	RAATTY	9 cut(s)	711, 788, 961, 1335, 1505, 1635, 1686, 1728, 1994
AcuI	CTGAAG	2 cut(s)	632, 709
AcvI	CACGTG	1 cut(s)	1663
AcyI	GRCGYC	1 cut(s)	56
AfaI	GTAC	6 cut(s)	227, 881, 1195, 1322, 1379, 1809
AfiI	CCNNNNNNNGG	6 cut(s)	100, 490, 746, 1152, 1367, 1723
AflII	CTTAAG	1 cut(s)	1162
AgeI	ACCGGT	1 cut(s)	1610
AgsI	TTSAA	4 cut(s)	191, 391, 794, 1990
AjnI	CCWGG	1 cut(s)	1267
AjuI	GAANNNNNNNTTGG	4 cut(s)	419, 451, 1779, 1811
Alw21I	GWGCWC	2 cut(s)	762, 1535
Alw26I	GTCTC	5 cut(s)	385, 526, 801, 1014, 1743
AlwI	GGATC	5 cut(s)	683, 689, 696, 1259, 1352
AlwNI	CAGNNNCTG	2 cut(s)	1274, 1829
AoxI	GGCC	3 cut(s)	69, 367, 1605
ApeKI	GCWGC	4 cut(s)	468, 752, 1562, 1643
ApoI	RAATTY	9 cut(s)	711, 788, 961, 1335, 1505, 1635, 1686, 1728, 1994
ArsI	GACNNNNNNTTYG	4 cut(s)	764, 796, 1838, 1870
AsiGI	ACCGGT	1 cut(s)	1610
Asp700I	GAANNNNTTC	1 cut(s)	1994
AspLEI	GCGC	4 cut(s)	1012, 1597, 1891, 1967
AspS9I	GGNCC	1 cut(s)	367
AsuC2I	CCSGG	2 cut(s)	101, 740
AsuHPI	GGTGA	4 cut(s)	328, 597, 929, 1295
AsuII	TTCGAA	1 cut(s)	324
AsuNHI	GCTAGC	1 cut(s)	820
BaeI	ACNNNNGTAYC	2 cut(s)	291, 324
BamHI	GGATCC	1 cut(s)	688
BauI	CACGAG	3 cut(s)	1400, 1483, 1534
BbrPI	CACGTG	1 cut(s)	1663
BbsI	GAAGAC	1 cut(s)	1053
Bbv12I	GWGCWC	2 cut(s)	762, 1535
BbvI	GCAGC	4 cut(s)	455, 764, 1574, 1655
BccI	CCATC	4 cut(s)	347, 1184, 1567, 1911
BceAI	ACGGC	2 cut(s)	504, 1165
BciT130I	CCWGG	1 cut(s)	1269
BciVI	GTATCC	1 cut(s)	319
BclI	TGATCA	2 cut(s)	508, 577
BcnI	CCSGG	2 cut(s)	101, 740
BcoDI	GTCTC	5 cut(s)	385, 526, 801, 1014, 1743
BfaI	CTAG	4 cut(s)	590, 821, 1089, 1868
BfmI	CTRYAG	1 cut(s)	81
BfoI	RGCGCY	2 cut(s)	1892, 1968
BfrI	CTTAAG	1 cut(s)	1162
BfuI	GTATCC	1 cut(s)	319
BisI	GCNGC	8 cut(s)	72, 469, 628, 753, 1095, 1204, 1563, 1644
BlpI	GCTNAGC	1 cut(s)	1892
BlsI	GCNGC	8 cut(s)	73, 470, 629, 754, 1096, 1205, 1564, 1645
BmcAI	AGTACT	1 cut(s)	1809
Bme1390I	CCNGG	3 cut(s)	101, 740, 1269
BmgT120I	GGNCC	1 cut(s)	367
BmiI	GGNNCC	1 cut(s)	690
BmrFI	CCNGG	3 cut(s)	101, 740, 1269
BmrI	ACTGGG	1 cut(s)	1885
BmtI	GCTAGC	1 cut(s)	824
BmuI	ACTGGG	1 cut(s)	1885
BoxI	GACNNNNGTC	1 cut(s)	1904
BpiI	GAAGAC	1 cut(s)	1053
BplI	GAGNNNNNCTC	2 cut(s)	798, 830
Bpu1102I	GCTNAGC	1 cut(s)	1892
Bpu14I	TTCGAA	1 cut(s)	324
BpuEI	CTTGAG	3 cut(s)	824, 1371, 1622
BpuMI	CCSGG	2 cut(s)	101, 740
BsaAI	YACGTR	1 cut(s)	1663
BsaBI	GATNNNNATC	1 cut(s)	693
BsaHI	GRCGYC	1 cut(s)	56
BsaJI	CCNNGG	4 cut(s)	448, 486, 1116, 1181
BsaWI	WCCGGW	2 cut(s)	220, 1610
BsaXI	ACNNNNNCTCC	4 cut(s)	404, 434, 1994, 2024
Bsc4I	CCNNNNNNNGG	6 cut(s)	100, 490, 746, 1152, 1367, 1723
Bse118I	RCCGGY	2 cut(s)	1610, 1885
Bse1I	ACTGG	5 cut(s)	950, 1086, 1823, 1860, 1880
Bse8I	GATNNNNATC	1 cut(s)	693
BseBI	CCWGG	1 cut(s)	1269
BseDI	CCNNGG	4 cut(s)	448, 486, 1116, 1181
BseGI	GGATG	8 cut(s)	161, 314, 599, 632, 826, 1195, 1922, 1953
BseJI	GATNNNNATC	1 cut(s)	693
BseLI	CCNNNNNNNGG	6 cut(s)	100, 490, 746, 1152, 1367, 1723
BseMII	CTCAG	6 cut(s)	478, 696, 1445, 1821, 1906, 2029
BseNI	ACTGG	5 cut(s)	950, 1086, 1823, 1860, 1880
BseRI	GAGGAG	4 cut(s)	146, 412, 1133, 1689
BseXI	GCAGC	4 cut(s)	455, 764, 1574, 1655
BsgI	GTGCAG	1 cut(s)	771
Bsh1236I	CGCG	4 cut(s)	450, 1450, 1595, 1850
BshFI	GGCC	3 cut(s)	71, 369, 1607
BshTI	ACCGGT	1 cut(s)	1610
BsiHKAI	GWGCWC	2 cut(s)	762, 1535
BsiSI	CCGG	6 cut(s)	101, 221, 686, 740, 1611, 1886
BslI	CCNNNNNNNGG	6 cut(s)	100, 490, 746, 1152, 1367, 1723
BsmAI	GTCTC	5 cut(s)	385, 526, 801, 1014, 1743
BsmBI	CGTCTC	1 cut(s)	1014
BsnI	GGCC	3 cut(s)	71, 369, 1607
Bsp119I	TTCGAA	1 cut(s)	324
Bsp1286I	GDGCHC	2 cut(s)	762, 1535
Bsp1720I	GCTNAGC	1 cut(s)	1892
BspANI	GGCC	3 cut(s)	71, 369, 1607
BspCNI	CTCAG	6 cut(s)	477, 697, 1446, 1822, 1905, 2028
BspFNI	CGCG	4 cut(s)	450, 1450, 1595, 1850
BspLI	GGNNCC	1 cut(s)	690
BspOI	GCTAGC	1 cut(s)	824
BspPI	GGATC	5 cut(s)	683, 689, 696, 1259, 1352
BspT104I	TTCGAA	1 cut(s)	324
BspTI	CTTAAG	1 cut(s)	1162
BsrBI	CCGCTC	1 cut(s)	1094
BsrFI	RCCGGY	2 cut(s)	1610, 1885
BsrI	ACTGG	5 cut(s)	950, 1086, 1823, 1860, 1880
BssAI	RCCGGY	2 cut(s)	1610, 1885
BssECI	CCNNGG	4 cut(s)	448, 486, 1116, 1181
BssNAI	GTATAC	1 cut(s)	595
BssNI	GRCGYC	1 cut(s)	56
BssSI	CACGAG	3 cut(s)	1400, 1483, 1534
Bst1107I	GTATAC	1 cut(s)	595
Bst2BI	CACGAG	3 cut(s)	1400, 1483, 1534
Bst2UI	CCWGG	1 cut(s)	1269
Bst4CI	ACNGT	4 cut(s)	118, 304, 862, 2042
Bst6I	CTCTTC	2 cut(s)	265, 651
BstACI	GRCGYC	1 cut(s)	56
BstAFI	CTTAAG	1 cut(s)	1162
BstBAI	YACGTR	1 cut(s)	1663
BstBI	TTCGAA	1 cut(s)	324
BstC8I	GCNNGC	6 cut(s)	7, 11, 69, 822, 1104, 1757
BstDEI	CTNAG	8 cut(s)	464, 552, 705, 1034, 1454, 1830, 1892, 2015
BstDSI	CCRYGG	3 cut(s)	448, 486, 1181
BstF5I	GGATG	8 cut(s)	161, 314, 599, 632, 826, 1195, 1922, 1953
BstFNI	CGCG	4 cut(s)	450, 1450, 1595, 1850
BstH2I	RGCGCY	2 cut(s)	1892, 1968
BstHHI	GCGC	4 cut(s)	1012, 1597, 1891, 1967
BstMAI	GTCTC	5 cut(s)	385, 526, 801, 1014, 1743
BstNI	CCWGG	1 cut(s)	1269
BstNSI	RCATGY	1 cut(s)	13
BstPAI	GACNNNNGTC	1 cut(s)	1904
BstSCI	CCNGG	3 cut(s)	99, 738, 1267
BstSFI	CTRYAG	1 cut(s)	81
BstUI	CGCG	4 cut(s)	450, 1450, 1595, 1850
BstV1I	GCAGC	4 cut(s)	455, 764, 1574, 1655
BstV2I	GAAGAC	1 cut(s)	1053
BstX2I	RGATCY	1 cut(s)	688
BstXI	CCANNNNNNTGG	1 cut(s)	957
BstYI	RGATCY	1 cut(s)	688
BstZ17I	GTATAC	1 cut(s)	595
BsuI	GTATCC	1 cut(s)	319
BsuRI	GGCC	3 cut(s)	71, 369, 1607
BtgI	CCRYGG	3 cut(s)	448, 486, 1181
BtsCI	GGATG	8 cut(s)	161, 314, 599, 632, 826, 1195, 1922, 1953
BtsI	GCAGTG	1 cut(s)	1616
BtsIMutI	CAGTG	2 cut(s)	1616, 1867
Cac8I	GCNNGC	6 cut(s)	7, 11, 69, 822, 1104, 1757
CaiI	CAGNNNCTG	2 cut(s)	1274, 1829
CfoI	GCGC	4 cut(s)	1012, 1597, 1891, 1967
Cfr10I	RCCGGY	2 cut(s)	1610, 1885
Cfr13I	GGNCC	1 cut(s)	367
Cfr42I	CCGCGG	1 cut(s)	451
CseI	GACGC	5 cut(s)	45, 1037, 1439, 1601, 1856
Csp6I	GTAC	6 cut(s)	226, 880, 1194, 1321, 1378, 1808
CspAI	ACCGGT	1 cut(s)	1610
CspCI	CAANNNNNGTGG	2 cut(s)	1637, 1672
CviAII	CATG	6 cut(s)	10, 374, 585, 637, 817, 1365
CviQI	GTAC	6 cut(s)	226, 880, 1194, 1321, 1378, 1808
DdeI	CTNAG	8 cut(s)	464, 552, 705, 1034, 1454, 1830, 1892, 2015
Eam1104I	CTCTTC	2 cut(s)	265, 651
EarI	CTCTTC	2 cut(s)	265, 651
EciI	GGCGGA	1 cut(s)	819
Eco57I	CTGAAG	2 cut(s)	632, 709
Eco72I	CACGTG	1 cut(s)	1663
EcoRI	GAATTC	4 cut(s)	711, 961, 1505, 1994
EcoRII	CCWGG	1 cut(s)	1267
Esp3I	CGTCTC	1 cut(s)	1014
FaeI	CATG	6 cut(s)	13, 377, 588, 640, 820, 1368
FatI	CATG	6 cut(s)	9, 373, 584, 636, 816, 1364
FauI	CCCGC	3 cut(s)	1483, 1748, 1843
FbaI	TGATCA	2 cut(s)	508, 577
FblI	GTMKAC	2 cut(s)	59, 594
Fnu4HI	GCNGC	8 cut(s)	72, 469, 628, 753, 1095, 1204, 1563, 1644
FokI	GGATG	8 cut(s)	148, 301, 586, 619, 813, 1202, 1929, 1960
Fsp4HI	GCNGC	8 cut(s)	72, 469, 628, 753, 1095, 1204, 1563, 1644
FspBI	CTAG	4 cut(s)	590, 821, 1089, 1868
GlaI	GCGC	4 cut(s)	1011, 1596, 1890, 1966
GluI	GCNGC	8 cut(s)	72, 469, 628, 753, 1095, 1204, 1563, 1644
HaeII	RGCGCY	2 cut(s)	1892, 1968
HaeIII	GGCC	3 cut(s)	71, 369, 1607
HapII	CCGG	6 cut(s)	101, 221, 686, 740, 1611, 1886
HgaI	GACGC	5 cut(s)	45, 1037, 1439, 1601, 1856
HhaI	GCGC	4 cut(s)	1012, 1597, 1891, 1967
Hin1I	GRCGYC	1 cut(s)	56
Hin1II	CATG	6 cut(s)	13, 377, 588, 640, 820, 1368
Hin6I	GCGC	4 cut(s)	1010, 1595, 1889, 1965
HinP1I	GCGC	4 cut(s)	1010, 1595, 1889, 1965
HincII	GTYRAC	4 cut(s)	60, 642, 1027, 1500
HindII	GTYRAC	4 cut(s)	60, 642, 1027, 1500
HindIII	AAGCTT	3 cut(s)	842, 1389, 1984
HinfI	GANTC	9 cut(s)	61, 920, 986, 1435, 1522, 1681, 1800, 1905, 2035
HpaI	GTTAAC	1 cut(s)	642
HpaII	CCGG	6 cut(s)	101, 221, 686, 740, 1611, 1886
HphI	GGTGA	4 cut(s)	328, 597, 929, 1295
Hpy166II	GTNNAC	8 cut(s)	60, 595, 642, 1027, 1238, 1500, 1616, 1873
Hpy188III	TCNNGA	6 cut(s)	650, 692, 716, 1019, 1526, 1705
Hpy8I	GTNNAC	8 cut(s)	60, 595, 642, 1027, 1238, 1500, 1616, 1873
Hpy99I	CGWCG	6 cut(s)	61, 308, 1020, 1595, 2003, 2006
HpyAV	CCTTC	6 cut(s)	148, 212, 1702, 1907, 1915, 1955
HpyCH4III	ACNGT	4 cut(s)	118, 304, 862, 2042
HpyCH4IV	ACGT	2 cut(s)	1023, 1662
HpyCH4V	TGCA	7 cut(s)	752, 868, 1219, 1364, 1480, 1562, 1937
HpyF3I	CTNAG	8 cut(s)	464, 552, 705, 1034, 1454, 1830, 1892, 2015
HpySE526I	ACGT	2 cut(s)	1023, 1662
Hsp92I	GRCGYC	1 cut(s)	56
Hsp92II	CATG	6 cut(s)	13, 377, 588, 640, 820, 1368
HspAI	GCGC	4 cut(s)	1010, 1595, 1889, 1965
Ksp22I	TGATCA	2 cut(s)	508, 577
KspAI	GTTAAC	1 cut(s)	642
KspI	CCGCGG	1 cut(s)	451
Lsp1109I	GCAGC	4 cut(s)	455, 764, 1574, 1655
MaeI	CTAG	4 cut(s)	590, 821, 1089, 1868
MaeII	ACGT	2 cut(s)	1023, 1662
MaeIII	GTNAC	6 cut(s)	403, 734, 952, 1243, 1825, 1906
MbiI	CCGCTC	1 cut(s)	1094
MboII	GAAGA	7 cut(s)	9, 133, 252, 334, 638, 1053, 1987
MflI	RGATCY	1 cut(s)	688
MhlI	GDGCHC	2 cut(s)	762, 1535
MlyI	GAGTC	3 cut(s)	55, 1690, 1914
MmeI	TCCRAC	4 cut(s)	408, 450, 949, 1167
MroXI	GAANNNNTTC	1 cut(s)	1994
MseI	TTAA	5 cut(s)	617, 641, 876, 1163, 1548
MspA1I	CMGCKG	6 cut(s)	450, 468, 627, 755, 1262, 1755
MspCI	CTTAAG	1 cut(s)	1162
MspI	CCGG	6 cut(s)	101, 221, 686, 740, 1611, 1886
MspR9I	CCNGG	3 cut(s)	101, 740, 1269
MvaI	CCWGG	1 cut(s)	1269
MvnI	CGCG	4 cut(s)	450, 1450, 1595, 1850
NciI	CCSGG	2 cut(s)	101, 740
NheI	GCTAGC	1 cut(s)	820
NlaIII	CATG	6 cut(s)	13, 377, 588, 640, 820, 1368
NlaIV	GGNNCC	1 cut(s)	690
NmeAIII	GCCGAG	2 cut(s)	503, 1141
NmuCI	GTSAC	3 cut(s)	403, 952, 1906
NspI	RCATGY	1 cut(s)	13
NspV	TTCGAA	1 cut(s)	324
PaeI	GCATGC	1 cut(s)	13
PcsI	WCGNNNNNNNCGW	2 cut(s)	36, 567
PdmI	GAANNNNTTC	1 cut(s)	1994
PfeI	GAWTC	6 cut(s)	920, 986, 1435, 1522, 1800, 2035
PfoI	TCCNGGA	1 cut(s)	99
PinAI	ACCGGT	1 cut(s)	1610
PkrI	GCNGC	8 cut(s)	73, 470, 629, 754, 1096, 1205, 1564, 1645
PleI	GAGTC	3 cut(s)	55, 1689, 1913
PmaCI	CACGTG	1 cut(s)	1663
PmlI	CACGTG	1 cut(s)	1663
PpsI	GAGTC	3 cut(s)	55, 1689, 1913
Ppu21I	YACGTR	1 cut(s)	1663
PshAI	GACNNNNGTC	1 cut(s)	1904
PsiI	TTATAA	1 cut(s)	1473
Psp6I	CCWGG	1 cut(s)	1267
PspCI	CACGTG	1 cut(s)	1663
PspGI	CCWGG	1 cut(s)	1267
PspN4I	GGNNCC	1 cut(s)	690
PspPI	GGNCC	1 cut(s)	367
PstNI	CAGNNNCTG	2 cut(s)	1274, 1829
PsuI	RGATCY	1 cut(s)	688
PvuII	CAGCTG	3 cut(s)	468, 755, 1262
RsaI	GTAC	6 cut(s)	227, 881, 1195, 1322, 1379, 1809
RsaNI	GTAC	6 cut(s)	226, 880, 1194, 1321, 1378, 1808
SacII	CCGCGG	1 cut(s)	451
SalI	GTCGAC	1 cut(s)	58
SaqAI	TTAA	5 cut(s)	617, 641, 876, 1163, 1548
SatI	GCNGC	8 cut(s)	72, 469, 628, 753, 1095, 1204, 1563, 1644
Sau96I	GGNCC	1 cut(s)	367
ScaI	AGTACT	1 cut(s)	1809
SchI	GAGTC	3 cut(s)	55, 1690, 1914
ScrFI	CCNGG	3 cut(s)	101, 740, 1269
SduI	GDGCHC	2 cut(s)	762, 1535
SfcI	CTRYAG	1 cut(s)	81
Sfr303I	CCGCGG	1 cut(s)	451
SfuI	TTCGAA	1 cut(s)	324
SgrBI	CCGCGG	1 cut(s)	451
SmlI	CTYRAG	4 cut(s)	839, 1162, 1386, 1601
SmoI	CTYRAG	4 cut(s)	839, 1162, 1386, 1601
SphI	GCATGC	1 cut(s)	13
SspMI	CTAG	4 cut(s)	590, 821, 1089, 1868
StyD4I	CCNGG	3 cut(s)	99, 738, 1267
TaaI	ACNGT	4 cut(s)	118, 304, 862, 2042
TaiI	ACGT	2 cut(s)	1026, 1665
TaqI	TCGA	8 cut(s)	59, 324, 503, 570, 1018, 1433, 1953, 1998
TatI	WGTACW	4 cut(s)	225, 879, 1377, 1807
TauI	GCSGC	4 cut(s)	74, 630, 1097, 1206
TfiI	GAWTC	6 cut(s)	920, 986, 1435, 1522, 1800, 2035
Tru1I	TTAA	5 cut(s)	617, 641, 876, 1163, 1548
Tru9I	TTAA	5 cut(s)	617, 641, 876, 1163, 1548
TscAI	CASTG	2 cut(s)	1623, 1867
TseFI	GTSAC	3 cut(s)	403, 952, 1906
TseI	GCWGC	4 cut(s)	468, 752, 1562, 1643
Tsp45I	GTSAC	3 cut(s)	403, 952, 1906
TspDTI	ATGAA	4 cut(s)	547, 978, 1053, 1301
TspGWI	ACGGA	4 cut(s)	955, 1224, 1330, 1925
TspRI	CASTG	2 cut(s)	1623, 1867
Vha464I	CTTAAG	1 cut(s)	1162
XapI	RAATTY	9 cut(s)	711, 788, 961, 1335, 1505, 1635, 1686, 1728, 1994
XceI	RCATGY	1 cut(s)	13
XmiI	GTMKAC	2 cut(s)	59, 594
XmnI	GAANNNNTTC	1 cut(s)	1994
XspI	CTAG	4 cut(s)	590, 821, 1089, 1868
ZrmI	AGTACT	1 cut(s)	1809

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.