Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000403

Physical Location & Seq

Genomic Coordinates Reverse (-)

9304095 .. 9306149

Gene Structure 2055 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1128600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2055 bp

ATGCTCTGTTCTCTTGCTCTCTTCGTCTGCATAGTCTGTTTTTCCGCTTTGATTCCGCAGGCCTCCCCCGCCCCATTCCTCTTCCCGGAAGCTCAATCCACCGTCCTCCCCGACCCTTCAACCTTCTTCGCCCCAAAACTTCTCTCTACTCCACTCCCCACCAACTCTTTCTTCCAAAACTTTGTCCTCAACAATGGCGACCAACCCGAGTACATCCATCCCTACACCATCAAATCATCCAACTCCTCCCTATCTATCACATATCCGTCTTTCTCCTACACATCTACCGTCATATCCACAGCCCCATTCGAACCCGACCTCACCATATCTGCCTCGAAAACCACCACTGGAAAAGCTCCAAACAACGACCACCATGTAATCTCTGACTTCAATGATCTCAGTGTGACATTGGACTTTCCCTCATCCAACCTCCGCTTCTTCCTTGTCCGCGGCAGCCCCTTTCTTACCTGTTTTCTTTATCGCCCCTCTGCGCTTACTATATCAACTGCTCATACAATTGTCTCATCATCATCAGCTAGTAGCTCAAAAACAAAGTTCATCATCAAGCTCGACAACAATCAGACATGGCTAATATACACATCCATTCCAACTGATTTATCTGACAGCGGATCATCCACACTAAATTTTAAAGCTTTTTCCGGGATTATTCGATTTGCTGTAATACCGGATCCTGACCCAAAATCAGAGGCAATTCTCGATCACTTCAGTTCTTGTTACCCAGTTTCTGGTGCAGCTGTGCTCCAAAACACCAATACTTTGACATATAAATGGGAAAAGGAAGGTGGAGATTTGCTTATGCTTGCTCATCCTCTCCATATTAAGCTTCTGTCCAATGCTACTGTTTTGCAAGATTTTAGATATAAAAGCATTGACGGCGATTTAGTTGGTGTTGTTGGCGGTTCGTGGATGTTAGCATCGAAGCCTATTCCAGTCACTTGGAATTCTGTAAGGGGTGTCCAAAAAGATTCGTTCCCCGAAATCATATCTGCACTTCGTCGAGATGTTGACGCTCTTAGTTCAGCAGAAGCTTCTACTACATCTTCATATTTTTACGGGAAATTGGTTGCTAGAGCAGCAAGGCTGGCTGTGATAGCCGAGGAGGTAGCTTGTCCTGATGTGACCCCGGCGATCAGGACATTCTTGGCGGAGAAAATAGAGCCATGGTTGGATGGCAGCTTTAGTGGCAACGGATTTTTGCATGATAAAAAATGGGGTGGACTTGTTACCAAGCAAGGATTAACTGATCAGGGTGCCGATTTCGGGTTTGGCGTTTATGCTGATCACCATTACCATCTGGGGTACTTTATATATGGAATTTCCGTGTTAGCAAAGATTGATCCTCCATGGAGGCAGAAGTACAAGTCTCAAGCTTACTCGCTCGTGGAGGATTTTCTCAACAAGGATGAACGATCGAATCCAAATTACCCGCGTCTGAGATCCTTTGATCTCTATAAATTGCACTCGTGGGCAGGTGGGTTGACAGAATTCGCAGACGGGAGGAATCAAGAGAGCACGAGCGAGGCGGTCAATGCTTACTACGCAGCTGCTTTGATGGGAAAAGCCTATAAAGATGCGCAACTTGAGGCCACCGGATCGCTACTCACTGCCTTGGAAATACAGGCAGCTCAGATGTGGTGGCACGTAAGAGAGGGCGGAGAGAATCGAATTTACCAGGAAGCTTTCGCAAAGGAAAATCGAGTAGTGGGGGTTTTATGGGCGACCAAGAGAGACACCGGACTTTGGTTCGCTACCGCGGAGGCGAGAGAAATTAGACTCGGAATTCAAATGCTGCCCGTTTTGCCAATCACCGAGGTGTTGTTCTCCGATGCAGGGTATGCCAACGAGCTCGTGAGTTGGGCATTGCCAGCGCTCAATAAAACAGAAGTCACGGAAGGATGGAAGGGATTTGTGCATGCGTTACAAGGGATTTACGACACACAAGGCGCTTTGAAGAAGATTAGGAGCTTGAATAGTTTCGACGACGGAAACTCCCGGAGTAATCTTTTGTGGTGGATTTACAGTCAGTCACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0000746 GO:0000747 GO:0000920 GO:0000935 GO:0000936 GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005937 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006076 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0009986 GO:0009987 GO:0010383 GO:0015629 GO:0015926 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016998 GO:0019953 GO:0022411 GO:0022414 GO:0030246 GO:0030247 GO:0030427 GO:0030428 GO:0030994 GO:0031505 GO:0032153 GO:0032155 GO:0042973 GO:0042995 GO:0043170 GO:0043187 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043332 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044277 GO:0044347 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044457 GO:0044463 GO:0044464 GO:0044703 GO:0044764 GO:0045229 GO:0051273 GO:0051275 GO:0051286 GO:0051301 GO:0051704 GO:0070871 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0071853 GO:0120025 GO:0120038 GO:1901575 GO:1904541 GO:1990819

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_81

Protein Analysis

685

Amino Acids

75.58

Weight (kDa)

5.91

Isoelectric Point (pI)

36.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_81	PF03639	49 - 319	1.6e-56	Glycosyl hydrolase family 81 N-terminal domain
Glyco_hydro81C	PF17652	327 - 678	2.4e-103	Glycosyl hydrolase family 81 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18310 AT5G15870
fragaria_vesca	FvH4_3g44967 FvH4_6g45580 FvH4_6g47860 FvH4_6g47870 FvH4_6g53160
malus_domestica	MD09G1047100.v1.1 MD09G1081400.v1.1 MD09G1081500.v1.1 MD09G1081600.v1.1 MD09G1081700.v1.1 MD15G1128600.v1.1 MD17G1047200.v1.1 MD17G1048700.v1.1 MD17G1071200.v1.1 MD17G1072700.v1.1
prunus_persica	Prupe.3G242000_v2.0.a1 Prupe.3G242100_v2.0.a1 Prupe.3G261700_v2.0.a1 Prupe.8G191700_v2.0.a1 Prupe.8G194600_v2.0.a1 Prupe.8G259500_v2.0.a1
pyrus_communis	pycom09g00810 pycom09g00820 pycom09g00830 pycom15g11570 pycom17g04350 pycom17g07070 pycom17g07350
rosa_chinensis	RchiOBHm_Chr1g0366281 RchiOBHm_Chr2g0163531 RchiOBHm_Chr2g0163581 RchiOBHm_Chr2g0167061 RchiOBHm_Chr2g0167071 RchiOBHm_Chr3g0490821 RchiOBHm_Chr3g0490841 RchiOBHm_Chr3g0492161 RchiOBHm_Chr3g0492171
rosa_laevigata	RLG00000021408 RLG00000021693 RLG00000021694 RLG00000022819 RLG00000022827
rosa_multiflora	Rmu_co8304887.1_g000001 Rmu_co8334235.1_g000001 Rmu_co8488335.1_g000001 Rmu_sc0003484.1_g000001 Rmu_sc0003484.1_g000004 Rmu_sc0010966.1_g000013 Rmu_sc0012462.1_g000001 Rmu_sc0019504.1_g000002 Rmu_sc0019504.1_g000004 Rmu_sc0022540.1_g000001 Rmu_sc0023760.1_g000001 Rmu_sc0038280.1_g000002
rosa_roxburghii	Rroxscaffold_2G00077500 Rroxscaffold_2G00083750 Rroxscaffold_2G00083760 Rroxscaffold_2G00087160 Rroxscaffold_2G00132530 Rroxscaffold_4G00290030 Rroxscaffold_4G00290040 Rroxscaffold_4G00290280
rosa_rugosa	Rorug01G0328400 Rorug02G0265200 Rorug02G0265900 Rorug02G0266000 Rorug02G0266800.1 Rorug02G0266900.1 Rorug02G0267500 Rorug02G0267600 Rorug02G0268300 Rorug02G0268800 Rorug02G0268900 Rorug02G0503600 Rorug02G0528200 Rorug02G0528300 Rorug03G0247200 Rorug03G0247300
rosa_samantha	Rh1BG298400 Rh1DG329800 Rh2AG570500 Rh2AG570700 Rh2AG595000 Rh2AG595100 Rh2AG595200 Rh2CG552500 Rh2CG552700 Rh2CG577100 Rh2CG577200 Rh2DG592500 Rh2DG617800 Rh2DG617900 Rh3CG333000 Rh3DG335000
rosa_wichuraiana	Rw2G047230 Rw2G049530 Rw2G049540 Rw3G026510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1481
Acc16I	TGCGCA	1 cut(s)	1596
Acc36I	ACCTGC	1 cut(s)	1481
AccB1I	GGYRCC	1 cut(s)	1271
AccB7I	CCANNNNNTGG	1 cut(s)	746
AccII	CGCG	3 cut(s)	450, 1450, 1775
AclWI	GGATC	6 cut(s)	637, 683, 696, 1352, 1452, 1621
AcsI	RAATTY	6 cut(s)	643, 961, 1335, 1505, 1686, 1800
AcuI	CTGAAG	1 cut(s)	709
AfaI	GTAC	3 cut(s)	212, 1322, 1379
AfeI	AGCGCT	1 cut(s)	1890
AfiI	CCNNNNNNNGG	5 cut(s)	85, 746, 1281, 1761, 1851
AgsI	TTSAA	5 cut(s)	120, 391, 1805, 1972, 1990
AjnI	CCWGG	1 cut(s)	1692
AjuI	GAANNNNNNNTTGG	4 cut(s)	155, 187, 419, 451
AleI	CACNNNNGTG	1 cut(s)	1832
Alw21I	GWGCWC	3 cut(s)	762, 1535, 1869
Alw26I	GTCTC	3 cut(s)	526, 1389, 1743
AlwI	GGATC	6 cut(s)	637, 683, 696, 1352, 1452, 1621
AlwNI	CAGNNNCTG	1 cut(s)	746
Ama87I	CYCGRG	1 cut(s)	206
Aor51HI	AGCGCT	1 cut(s)	1890
AoxI	GGCC	2 cut(s)	60, 1605
ApeKI	GCWGC	8 cut(s)	453, 752, 1094, 1194, 1562, 1565, 1643, 1810
ApoI	RAATTY	6 cut(s)	643, 961, 1335, 1505, 1686, 1800
Asp700I	GAANNNNTTC	1 cut(s)	1994
AspLEI	GCGC	4 cut(s)	493, 1597, 1891, 1967
AsuC2I	CCSGG	4 cut(s)	86, 661, 1145, 2014
AsuHPI	GGTGA	3 cut(s)	313, 1295, 1819
AsuII	TTCGAA	1 cut(s)	309
AvaI	CYCGRG	1 cut(s)	206
BamHI	GGATCC	1 cut(s)	688
BanI	GGYRCC	1 cut(s)	1271
BanII	GRGCYC	1 cut(s)	1869
BauI	CACGAG	4 cut(s)	1400, 1483, 1534, 1868
Bbv12I	GWGCWC	3 cut(s)	762, 1535, 1869
BbvI	GCAGC	8 cut(s)	465, 764, 1106, 1206, 1552, 1574, 1655, 1797
BccI	CCATC	6 cut(s)	225, 236, 1184, 1320, 1567, 1911
BceAI	ACGGC	1 cut(s)	910
BciT130I	CCWGG	1 cut(s)	1694
BclI	TGATCA	2 cut(s)	1264, 1300
BcnI	CCSGG	4 cut(s)	86, 661, 1145, 2014
BcoDI	GTCTC	3 cut(s)	526, 1389, 1743
BfaI	CTAG	2 cut(s)	537, 1089
BfoI	RGCGCY	2 cut(s)	1892, 1968
BfuAI	ACCTGC	1 cut(s)	1481
BisI	GCNGC	9 cut(s)	451, 454, 753, 1095, 1195, 1563, 1566, 1644, 1811
BlsI	GCNGC	9 cut(s)	452, 455, 754, 1096, 1196, 1564, 1567, 1645, 1812
Bme1390I	CCNGG	5 cut(s)	86, 661, 1145, 1694, 2014
BmeT110I	CYCGRG	1 cut(s)	206
BmiI	GGNNCC	2 cut(s)	690, 1273
BmrFI	CCNGG	5 cut(s)	86, 661, 1145, 1694, 2014
BmrI	ACTGGG	1 cut(s)	734
BmsI	GCATC	3 cut(s)	944, 1582, 1837
BmuI	ACTGGG	1 cut(s)	734
Bpu14I	TTCGAA	1 cut(s)	309
BpuEI	CTTGAG	2 cut(s)	1371, 1622
BpuMI	CCSGG	4 cut(s)	86, 661, 1145, 2014
BsaAI	YACGTR	1 cut(s)	1663
BsaJI	CCNNGG	8 cut(s)	448, 1116, 1143, 1181, 1364, 1629, 1773, 1830
BsaWI	WCCGGW	3 cut(s)	685, 1610, 1754
BsaXI	ACNNNNNCTCC	2 cut(s)	1994, 2024
Bsc4I	CCNNNNNNNGG	5 cut(s)	85, 746, 1281, 1761, 1851
Bse1I	ACTGG	3 cut(s)	352, 740, 950
Bse3DI	GCAATG	1 cut(s)	1880
BseBI	CCWGG	1 cut(s)	1694
BseDI	CCNNGG	8 cut(s)	448, 1116, 1143, 1181, 1364, 1629, 1773, 1830
BseLI	CCNNNNNNNGG	5 cut(s)	85, 746, 1281, 1761, 1851
BseMI	GCAATG	1 cut(s)	1880
BseMII	CTCAG	3 cut(s)	412, 1445, 1661
BseNI	ACTGG	3 cut(s)	352, 740, 950
BseRI	GAGGAG	2 cut(s)	235, 1133
BseXI	GCAGC	8 cut(s)	465, 764, 1106, 1206, 1552, 1574, 1655, 1797
BsgI	GTGCAG	2 cut(s)	771, 993
Bsh1236I	CGCG	3 cut(s)	450, 1450, 1775
Bsh1285I	CGRYCG	1 cut(s)	1433
BshFI	GGCC	2 cut(s)	62, 1607
BshNI	GGYRCC	1 cut(s)	1271
BsiEI	CGRYCG	1 cut(s)	1433
BsiHKAI	GWGCWC	3 cut(s)	762, 1535, 1869
BsiHKCI	CYCGRG	1 cut(s)	206
BsiSI	CCGG	7 cut(s)	86, 660, 686, 1145, 1611, 1755, 2014
BslI	CCNNNNNNNGG	5 cut(s)	85, 746, 1281, 1761, 1851
BsmAI	GTCTC	3 cut(s)	526, 1389, 1743
BsnI	GGCC	2 cut(s)	62, 1607
BsoBI	CYCGRG	1 cut(s)	206
Bsp119I	TTCGAA	1 cut(s)	309
Bsp1286I	GDGCHC	3 cut(s)	762, 1535, 1869
Bsp19I	CCATGG	2 cut(s)	1181, 1364
BspANI	GGCC	2 cut(s)	62, 1607
BspCNI	CTCAG	3 cut(s)	411, 1446, 1660
BspFNI	CGCG	3 cut(s)	450, 1450, 1775
BspLI	GGNNCC	2 cut(s)	690, 1273
BspMI	ACCTGC	1 cut(s)	1481
BspPI	GGATC	6 cut(s)	637, 683, 696, 1352, 1452, 1621
BspT104I	TTCGAA	1 cut(s)	309
BspT107I	GGYRCC	1 cut(s)	1271
BsrDI	GCAATG	1 cut(s)	1880
BsrI	ACTGG	3 cut(s)	352, 740, 950
BssECI	CCNNGG	8 cut(s)	448, 1116, 1143, 1181, 1364, 1629, 1773, 1830
BssSI	CACGAG	4 cut(s)	1400, 1483, 1534, 1868
BssT1I	CCWWGG	3 cut(s)	1181, 1364, 1629
Bst2BI	CACGAG	4 cut(s)	1400, 1483, 1534, 1868
Bst2UI	CCWGG	1 cut(s)	1694
Bst4CI	ACNGT	5 cut(s)	103, 289, 862, 2042, 2052
Bst6I	CTCTTC	2 cut(s)	26, 86
BstAPI	GCANNNNNTGC	1 cut(s)	1856
BstBAI	YACGTR	1 cut(s)	1663
BstBI	TTCGAA	1 cut(s)	309
BstC8I	GCNNGC	5 cut(s)	60, 822, 1104, 1887, 1935
BstDEI	CTNAG	4 cut(s)	398, 1034, 1454, 1647
BstDSI	CCRYGG	4 cut(s)	448, 1181, 1364, 1773
BstFNI	CGCG	3 cut(s)	450, 1450, 1775
BstH2I	RGCGCY	2 cut(s)	1892, 1968
BstHHI	GCGC	4 cut(s)	493, 1597, 1891, 1967
BstMAI	GTCTC	3 cut(s)	526, 1389, 1743
BstMCI	CGRYCG	1 cut(s)	1433
BstNI	CCWGG	1 cut(s)	1694
BstNSI	RCATGY	1 cut(s)	1937
BstSCI	CCNGG	5 cut(s)	84, 659, 1143, 1692, 2012
BstUI	CGCG	3 cut(s)	450, 1450, 1775
BstV1I	GCAGC	8 cut(s)	465, 764, 1106, 1206, 1552, 1574, 1655, 1797
BstX2I	RGATCY	2 cut(s)	688, 1457
BstXI	CCANNNNNNTGG	1 cut(s)	957
BstYI	RGATCY	2 cut(s)	688, 1457
BsuRI	GGCC	2 cut(s)	62, 1607
BtgI	CCRYGG	4 cut(s)	448, 1181, 1364, 1773
BtsI	GCAGTG	1 cut(s)	1623
BtsIMutI	CAGTG	3 cut(s)	345, 406, 1623
BveI	ACCTGC	1 cut(s)	1481
Cac8I	GCNNGC	5 cut(s)	60, 822, 1104, 1887, 1935
CaiI	CAGNNNCTG	1 cut(s)	746
CfoI	GCGC	4 cut(s)	493, 1597, 1891, 1967
Cfr42I	CCGCGG	2 cut(s)	451, 1776
CseI	GACGC	2 cut(s)	1037, 1439
Csp6I	GTAC	3 cut(s)	211, 1321, 1378
CviAII	CATG	6 cut(s)	374, 585, 1182, 1220, 1365, 1934
CviQI	GTAC	3 cut(s)	211, 1321, 1378
DdeI	CTNAG	4 cut(s)	398, 1034, 1454, 1647
DraI	TTTAAA	1 cut(s)	649
Eam1104I	CTCTTC	2 cut(s)	26, 86
EarI	CTCTTC	2 cut(s)	26, 86
EciI	GGCGGA	2 cut(s)	1181, 1689
Ecl136II	GAGCTC	1 cut(s)	1867
Eco130I	CCWWGG	3 cut(s)	1181, 1364, 1629
Eco147I	AGGCCT	1 cut(s)	62
Eco24I	GRGCYC	1 cut(s)	1869
Eco47III	AGCGCT	1 cut(s)	1890
Eco53kI	GAGCTC	1 cut(s)	1867
Eco57I	CTGAAG	1 cut(s)	709
Eco88I	CYCGRG	1 cut(s)	206
EcoICRI	GAGCTC	1 cut(s)	1867
EcoRI	GAATTC	3 cut(s)	961, 1505, 1800
EcoRII	CCWGG	1 cut(s)	1692
EcoT14I	CCWWGG	3 cut(s)	1181, 1364, 1629
EcoT38I	GRGCYC	1 cut(s)	1869
ErhI	CCWWGG	3 cut(s)	1181, 1364, 1629
FaeI	CATG	6 cut(s)	377, 588, 1185, 1223, 1368, 1937
FatI	CATG	6 cut(s)	373, 584, 1181, 1219, 1364, 1933
FauI	CCCGC	2 cut(s)	76, 1455
FbaI	TGATCA	2 cut(s)	1264, 1300
Fnu4HI	GCNGC	9 cut(s)	451, 454, 753, 1095, 1195, 1563, 1566, 1644, 1811
FriOI	GRGCYC	1 cut(s)	1869
Fsp4HI	GCNGC	9 cut(s)	451, 454, 753, 1095, 1195, 1563, 1566, 1644, 1811
FspBI	CTAG	2 cut(s)	537, 1089
FspI	TGCGCA	1 cut(s)	1596
GlaI	GCGC	4 cut(s)	492, 1596, 1890, 1966
GluI	GCNGC	9 cut(s)	451, 454, 753, 1095, 1195, 1563, 1566, 1644, 1811
HaeII	RGCGCY	2 cut(s)	1892, 1968
HaeIII	GGCC	2 cut(s)	62, 1607
HapII	CCGG	7 cut(s)	86, 660, 686, 1145, 1611, 1755, 2014
HgaI	GACGC	2 cut(s)	1037, 1439
HhaI	GCGC	4 cut(s)	493, 1597, 1891, 1967
Hin1II	CATG	6 cut(s)	377, 588, 1185, 1223, 1368, 1937
Hin6I	GCGC	4 cut(s)	491, 1595, 1889, 1965
HinP1I	GCGC	4 cut(s)	491, 1595, 1889, 1965
HincII	GTYRAC	2 cut(s)	1027, 1500
HindII	GTYRAC	2 cut(s)	1027, 1500
HindIII	AAGCTT	5 cut(s)	651, 842, 1047, 1389, 1698
HinfI	GANTC	6 cut(s)	52, 986, 1435, 1522, 1681, 1794
HpaII	CCGG	7 cut(s)	86, 660, 686, 1145, 1611, 1755, 2014
HphI	GGTGA	3 cut(s)	313, 1295, 1819
Hpy166II	GTNNAC	3 cut(s)	1027, 1238, 1500
Hpy188I	TCNGA	8 cut(s)	385, 582, 622, 706, 1455, 1650, 1799, 1846
Hpy188III	TCNNGA	7 cut(s)	692, 716, 1019, 1133, 1153, 1526, 1870
Hpy8I	GTNNAC	3 cut(s)	1027, 1238, 1500
Hpy99I	CGWCG	3 cut(s)	1020, 2003, 2006
HpyAV	CCTTC	5 cut(s)	126, 133, 794, 1907, 1915
HpyCH4III	ACNGT	5 cut(s)	103, 289, 862, 2042, 2052
HpyCH4IV	ACGT	1 cut(s)	1662
HpyCH4V	TGCA	8 cut(s)	30, 752, 868, 1010, 1219, 1480, 1850, 1933
HpyF3I	CTNAG	4 cut(s)	398, 1034, 1454, 1647
HpySE526I	ACGT	1 cut(s)	1662
Hsp92II	CATG	6 cut(s)	377, 588, 1185, 1223, 1368, 1937
HspAI	GCGC	4 cut(s)	491, 1595, 1889, 1965
Ksp22I	TGATCA	2 cut(s)	1264, 1300
KspI	CCGCGG	2 cut(s)	451, 1776
LmnI	GCTCC	3 cut(s)	361, 765, 1983
Lsp1109I	GCAGC	8 cut(s)	465, 764, 1106, 1206, 1552, 1574, 1655, 1797
LweI	GCATC	3 cut(s)	944, 1582, 1837
MaeI	CTAG	2 cut(s)	537, 1089
MaeII	ACGT	1 cut(s)	1662
MaeIII	GTNAC	8 cut(s)	403, 734, 952, 1138, 1243, 1906, 1938, 2046
MboII	GAAGA	8 cut(s)	13, 73, 118, 163, 430, 1053, 1984, 1987
MfeI	CAATTG	1 cut(s)	516
MflI	RGATCY	2 cut(s)	688, 1457
MhlI	GDGCHC	3 cut(s)	762, 1535, 1869
MlyI	GAGTC	1 cut(s)	1788
MmeI	TCCRAC	4 cut(s)	264, 450, 632, 1167
MroXI	GAANNNNTTC	1 cut(s)	1994
MseI	TTAA	3 cut(s)	648, 840, 1259
MslI	CAYNNNNRTG	2 cut(s)	787, 1832
MspA1I	CMGCKG	5 cut(s)	450, 627, 755, 1565, 1775
MspI	CCGG	7 cut(s)	86, 660, 686, 1145, 1611, 1755, 2014
MspR9I	CCNGG	5 cut(s)	86, 661, 1145, 1694, 2014
MunI	CAATTG	1 cut(s)	516
MvaI	CCWGG	1 cut(s)	1694
MvnI	CGCG	3 cut(s)	450, 1450, 1775
NciI	CCSGG	4 cut(s)	86, 661, 1145, 2014
NcoI	CCATGG	2 cut(s)	1181, 1364
NlaIII	CATG	6 cut(s)	377, 588, 1185, 1223, 1368, 1937
NlaIV	GGNNCC	2 cut(s)	690, 1273
NmeAIII	GCCGAG	1 cut(s)	1141
NmuCI	GTSAC	5 cut(s)	403, 952, 1138, 1906, 2046
NsbI	TGCGCA	1 cut(s)	1596
NspI	RCATGY	1 cut(s)	1937
NspV	TTCGAA	1 cut(s)	309
OliI	CACNNNNGTG	1 cut(s)	1832
PaeI	GCATGC	1 cut(s)	1937
PaqCI	CACCTGC	1 cut(s)	1481
PceI	AGGCCT	1 cut(s)	62
PcsI	WCGNNNNNNNCGW	1 cut(s)	1287
PdmI	GAANNNNTTC	1 cut(s)	1994
PfeI	GAWTC	5 cut(s)	52, 986, 1435, 1522, 1681
PflMI	CCANNNNNTGG	1 cut(s)	746
PfoI	TCCNGGA	3 cut(s)	84, 659, 2012
PkrI	GCNGC	9 cut(s)	452, 455, 754, 1096, 1196, 1564, 1567, 1645, 1812
Ple19I	CGATCG	1 cut(s)	1433
PleI	GAGTC	1 cut(s)	1788
PpsI	GAGTC	1 cut(s)	1788
Ppu21I	YACGTR	1 cut(s)	1663
Psp124BI	GAGCTC	1 cut(s)	1869
Psp6I	CCWGG	1 cut(s)	1692
PspGI	CCWGG	1 cut(s)	1692
PspN4I	GGNNCC	2 cut(s)	690, 1273
PstNI	CAGNNNCTG	1 cut(s)	746
PsuI	RGATCY	2 cut(s)	688, 1457
PvuI	CGATCG	1 cut(s)	1433
PvuII	CAGCTG	2 cut(s)	755, 1565
RsaI	GTAC	3 cut(s)	212, 1322, 1379
RsaNI	GTAC	3 cut(s)	211, 1321, 1378
RseI	CAYNNNNRTG	2 cut(s)	787, 1832
SacI	GAGCTC	1 cut(s)	1869
SacII	CCGCGG	2 cut(s)	451, 1776
SaqAI	TTAA	3 cut(s)	648, 840, 1259
SatI	GCNGC	9 cut(s)	451, 454, 753, 1095, 1195, 1563, 1566, 1644, 1811
SchI	GAGTC	1 cut(s)	1788
ScrFI	CCNGG	5 cut(s)	86, 661, 1145, 1694, 2014
SduI	GDGCHC	3 cut(s)	762, 1535, 1869
SfaNI	GCATC	3 cut(s)	944, 1582, 1837
Sfr303I	CCGCGG	2 cut(s)	451, 1776
SfuI	TTCGAA	1 cut(s)	309
SgrBI	CCGCGG	2 cut(s)	451, 1776
SmiMI	CAYNNNNRTG	2 cut(s)	787, 1832
SmlI	CTYRAG	2 cut(s)	1386, 1601
SmoI	CTYRAG	2 cut(s)	1386, 1601
SphI	GCATGC	1 cut(s)	1937
SseBI	AGGCCT	1 cut(s)	62
SspMI	CTAG	2 cut(s)	537, 1089
SstI	GAGCTC	1 cut(s)	1869
StuI	AGGCCT	1 cut(s)	62
StyD4I	CCNGG	5 cut(s)	84, 659, 1143, 1692, 2012
StyI	CCWWGG	3 cut(s)	1181, 1364, 1629
TaaI	ACNGT	5 cut(s)	103, 289, 862, 2042, 2052
TaiI	ACGT	1 cut(s)	1665
TatI	WGTACW	2 cut(s)	210, 1377
TauI	GCSGC	1 cut(s)	453
TfiI	GAWTC	5 cut(s)	52, 986, 1435, 1522, 1681
Tru1I	TTAA	3 cut(s)	648, 840, 1259
Tru9I	TTAA	3 cut(s)	648, 840, 1259
TscAI	CASTG	3 cut(s)	352, 406, 1630
TseFI	GTSAC	5 cut(s)	403, 952, 1138, 1906, 2046
TseI	GCWGC	8 cut(s)	453, 752, 1094, 1194, 1562, 1565, 1643, 1810
Tsp45I	GTSAC	5 cut(s)	403, 952, 1138, 1906, 2046
TspDTI	ATGAA	3 cut(s)	547, 1053, 1440
TspGWI	ACGGA	5 cut(s)	255, 1224, 1330, 1925, 2019
TspRI	CASTG	3 cut(s)	352, 406, 1630
Van91I	CCANNNNNTGG	1 cut(s)	746
XapI	RAATTY	6 cut(s)	643, 961, 1335, 1505, 1686, 1800
XceI	RCATGY	1 cut(s)	1937
XmnI	GAANNNNTTC	1 cut(s)	1994
XspI	CTAG	2 cut(s)	537, 1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.