Endo-1,3(4)-beta-glucanase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 677 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 413, 872 |
| AccBSI | CCGCTC | 1 cut(s) | 1031 |
| AccI | GTMKAC | 1 cut(s) | 788 |
| AccII | CGCG | 1 cut(s) | 503 |
| AclWI | GGATC | 3 cut(s) | 1551, 2044, 2057 |
| AcsI | RAATTY | 3 cut(s) | 1526, 1815, 1999 |
| AcuI | CTGAAG | 4 cut(s) | 244, 385, 830, 1158 |
| AcyI | GRCGYC | 3 cut(s) | 5, 414, 1064 |
| AfaI | GTAC | 5 cut(s) | 216, 324, 566, 605, 899 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 460, 749, 1387, 1959 |
| AflIII | ACRYGT | 1 cut(s) | 1701 |
| AgsI | TTSAA | 6 cut(s) | 62, 895, 952, 1461, 1655, 1997 |
| AjnI | CCWGG | 2 cut(s) | 694, 1380 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 431, 463, 1794, 1826 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1806, 1838 |
| AluBI | AGCT | 9 cut(s) | 71, 95, 258, 341, 850, 1375, 1412, 1667, 1869 |
| AluI | AGCT | 9 cut(s) | 71, 95, 258, 341, 850, 1375, 1412, 1667, 1869 |
| Alw21I | GWGCWC | 2 cut(s) | 1556, 1689 |
| Alw26I | GTCTC | 5 cut(s) | 397, 581, 629, 1605, 1758 |
| Alw44I | GTGCAC | 1 cut(s) | 1685 |
| AlwI | GGATC | 3 cut(s) | 1551, 2044, 2057 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 701 |
| AoxI | GGCC | 1 cut(s) | 39 |
| ApaLI | GTGCAC | 1 cut(s) | 1685 |
| ApeKI | GCWGC | 4 cut(s) | 1450, 1586, 1646, 1664 |
| ApoI | RAATTY | 3 cut(s) | 1526, 1815, 1999 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1289, 1321 |
| AseI | ATTAAT | 1 cut(s) | 987 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1232 |
| AspLEI | GCGC | 1 cut(s) | 505 |
| AspS9I | GGNCC | 1 cut(s) | 40 |
| AsuC2I | CCSGG | 1 cut(s) | 743 |
| AsuHPI | GGTGA | 7 cut(s) | 141, 523, 619, 764, 926, 1316, 1615 |
| AsuII | TTCGAA | 1 cut(s) | 1037 |
| AsuNHI | GCTAGC | 1 cut(s) | 826 |
| BaeGI | GKGCMC | 1 cut(s) | 1689 |
| BamHI | GGATCC | 1 cut(s) | 2049 |
| Bbv12I | GWGCWC | 2 cut(s) | 1556, 1689 |
| BbvI | GCAGC | 4 cut(s) | 1462, 1573, 1658, 1676 |
| BccI | CCATC | 5 cut(s) | 518, 905, 1529, 1588, 2012 |
| BceAI | ACGGC | 1 cut(s) | 1095 |
| BciT130I | CCWGG | 2 cut(s) | 696, 1382 |
| BclI | TGATCA | 2 cut(s) | 648, 1321 |
| BcnI | CCSGG | 1 cut(s) | 743 |
| BcoDI | GTCTC | 5 cut(s) | 397, 581, 629, 1605, 1758 |
| BfaI | CTAG | 4 cut(s) | 72, 719, 827, 1911 |
| BfmI | CTRYAG | 1 cut(s) | 253 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1115 |
| BisI | GCNGC | 6 cut(s) | 1029, 1116, 1451, 1587, 1647, 1665 |
| BlsI | GCNGC | 6 cut(s) | 1030, 1117, 1452, 1588, 1648, 1666 |
| Bme1390I | CCNGG | 3 cut(s) | 696, 743, 1382 |
| BmgT120I | GGNCC | 1 cut(s) | 40 |
| BmiI | GGNNCC | 1 cut(s) | 2051 |
| BmrFI | CCNGG | 3 cut(s) | 696, 743, 1382 |
| BmsI | GCATC | 6 cut(s) | 360, 1016, 1180, 1643, 1918, 1969 |
| BmtI | GCTAGC | 1 cut(s) | 830 |
| BpmI | CTGGAG | 3 cut(s) | 311, 1533, 1773 |
| Bpu10I | CCTNAGC | 1 cut(s) | 347 |
| Bpu14I | TTCGAA | 1 cut(s) | 1037 |
| BpuMI | CCSGG | 1 cut(s) | 743 |
| BsaHI | GRCGYC | 3 cut(s) | 5, 414, 1064 |
| BsaI | GGTCTC | 1 cut(s) | 629 |
| BsaJI | CCNNGG | 5 cut(s) | 409, 1110, 1202, 1690, 1953 |
| BsaWI | WCCGGW | 1 cut(s) | 749 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 416, 446, 526, 556, 596, 626 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 460, 749, 1387, 1959 |
| Bse118I | RCCGGY | 1 cut(s) | 1900 |
| Bse1I | ACTGG | 3 cut(s) | 968, 1774, 1887 |
| Bse3DI | GCAATG | 3 cut(s) | 490, 1023, 1637 |
| BseBI | CCWGG | 2 cut(s) | 696, 1382 |
| BseDI | CCNNGG | 5 cut(s) | 409, 1110, 1202, 1690, 1953 |
| BseGI | GGATG | 4 cut(s) | 275, 426, 832, 1195 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 460, 749, 1387, 1959 |
| BseMI | GCAATG | 3 cut(s) | 490, 1023, 1637 |
| BseMII | CTCAG | 3 cut(s) | 58, 1374, 1836 |
| BseNI | ACTGG | 3 cut(s) | 968, 1774, 1887 |
| BseRI | GAGGAG | 4 cut(s) | 161, 385, 424, 1776 |
| BseSI | GKGCMC | 1 cut(s) | 1689 |
| BseXI | GCAGC | 4 cut(s) | 1462, 1573, 1658, 1676 |
| BseYI | CCCAGC | 1 cut(s) | 1223 |
| Bsh1236I | CGCG | 1 cut(s) | 503 |
| BshFI | GGCC | 1 cut(s) | 41 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1556, 1689 |
| BsiSI | CCGG | 5 cut(s) | 98, 669, 743, 750, 1901 |
| BslFI | GGGAC | 2 cut(s) | 122, 1228 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 460, 749, 1387, 1959 |
| BsmAI | GTCTC | 5 cut(s) | 397, 581, 629, 1605, 1758 |
| BsmFI | GGGAC | 2 cut(s) | 122, 1228 |
| BsmI | GAATGC | 3 cut(s) | 979, 1501, 1576 |
| BsnI | GGCC | 1 cut(s) | 41 |
| Bso31I | GGTCTC | 1 cut(s) | 629 |
| Bsp119I | TTCGAA | 1 cut(s) | 1037 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1556, 1689 |
| Bsp143I | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| Bsp19I | CCATGG | 1 cut(s) | 1202 |
| BspANI | GGCC | 1 cut(s) | 41 |
| BspCNI | CTCAG | 3 cut(s) | 57, 1373, 1837 |
| BspFNI | CGCG | 1 cut(s) | 503 |
| BspHI | TCATGA | 1 cut(s) | 1434 |
| BspLI | GGNNCC | 1 cut(s) | 2051 |
| BspOI | GCTAGC | 1 cut(s) | 830 |
| BspPI | GGATC | 3 cut(s) | 1551, 2044, 2057 |
| BspT104I | TTCGAA | 1 cut(s) | 1037 |
| BspTNI | GGTCTC | 1 cut(s) | 629 |
| BsrBI | CCGCTC | 1 cut(s) | 1031 |
| BsrDI | GCAATG | 3 cut(s) | 490, 1023, 1637 |
| BsrFI | RCCGGY | 1 cut(s) | 1900 |
| BsrI | ACTGG | 3 cut(s) | 968, 1774, 1887 |
| BssAI | RCCGGY | 1 cut(s) | 1900 |
| BssECI | CCNNGG | 5 cut(s) | 409, 1110, 1202, 1690, 1953 |
| BssMI | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| BssNAI | GTATAC | 1 cut(s) | 789 |
| BssNI | GRCGYC | 3 cut(s) | 5, 414, 1064 |
| BssT1I | CCWWGG | 4 cut(s) | 1110, 1202, 1690, 1953 |
| Bst1107I | GTATAC | 1 cut(s) | 789 |
| Bst2UI | CCWGG | 2 cut(s) | 696, 1382 |
| Bst4CI | ACNGT | 3 cut(s) | 133, 731, 880 |
| Bst6I | CTCTTC | 3 cut(s) | 767, 843, 1134 |
| BstACI | GRCGYC | 3 cut(s) | 5, 414, 1064 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1571 |
| BstBI | TTCGAA | 1 cut(s) | 1037 |
| BstC8I | GCNNGC | 5 cut(s) | 103, 828, 1125, 1410, 1644 |
| BstDEI | CTNAG | 4 cut(s) | 44, 347, 1360, 1845 |
| BstDSI | CCRYGG | 1 cut(s) | 1202 |
| BstEII | GGTNACC | 1 cut(s) | 914 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 458 |
| BstF5I | GGATG | 4 cut(s) | 275, 426, 832, 1195 |
| BstFNI | CGCG | 1 cut(s) | 503 |
| BstHHI | GCGC | 1 cut(s) | 505 |
| BstKTI | GATC | 7 cut(s) | 651, 1023, 1324, 1489, 1546, 1737, 2052 |
| BstMAI | GTCTC | 5 cut(s) | 397, 581, 629, 1605, 1758 |
| BstMBI | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 314, 1115, 1124, 1133, 1571 |
| BstNI | CCWGG | 2 cut(s) | 696, 1382 |
| BstNSI | RCATGY | 3 cut(s) | 483, 1685, 1705 |
| BstPI | GGTNACC | 1 cut(s) | 914 |
| BstSCI | CCNGG | 3 cut(s) | 694, 741, 1380 |
| BstSFI | CTRYAG | 1 cut(s) | 253 |
| BstSLI | GKGCMC | 1 cut(s) | 1689 |
| BstUI | CGCG | 1 cut(s) | 503 |
| BstV1I | GCAGC | 4 cut(s) | 1462, 1573, 1658, 1676 |
| BstX2I | RGATCY | 1 cut(s) | 2049 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 975 |
| BstYI | RGATCY | 1 cut(s) | 2049 |
| BstZ17I | GTATAC | 1 cut(s) | 789 |
| BsuRI | GGCC | 1 cut(s) | 41 |
| BtgI | CCRYGG | 1 cut(s) | 1202 |
| BtsCI | GGATG | 4 cut(s) | 275, 426, 832, 1195 |
| BtsI | GCAGTG | 2 cut(s) | 1287, 1572 |
| BtsIMutI | CAGTG | 8 cut(s) | 409, 644, 876, 1287, 1368, 1572, 1767, 1842 |
| Cac8I | GCNNGC | 5 cut(s) | 103, 828, 1125, 1410, 1644 |
| CaiI | CAGNNNCTG | 1 cut(s) | 701 |
| CciI | TCATGA | 1 cut(s) | 1434 |
| CfoI | GCGC | 1 cut(s) | 505 |
| Cfr10I | RCCGGY | 1 cut(s) | 1900 |
| Cfr13I | GGNCC | 1 cut(s) | 40 |
| CseI | GACGC | 2 cut(s) | 403, 1072 |
| Csp6I | GTAC | 5 cut(s) | 215, 323, 565, 604, 898 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1344, 1379, 1658, 1693 |
| CviQI | GTAC | 5 cut(s) | 215, 323, 565, 604, 898 |
| DdeI | CTNAG | 4 cut(s) | 44, 347, 1360, 1845 |
| DpnI | GATC | 7 cut(s) | 650, 1022, 1323, 1488, 1545, 1736, 2051 |
| DpnII | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 413, 872 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 413, 872 |
| Eam1104I | CTCTTC | 3 cut(s) | 767, 843, 1134 |
| EarI | CTCTTC | 3 cut(s) | 767, 843, 1134 |
| EciI | GGCGGA | 1 cut(s) | 346 |
| Eco130I | CCWWGG | 4 cut(s) | 1110, 1202, 1690, 1953 |
| Eco31I | GGTCTC | 1 cut(s) | 629 |
| Eco57I | CTGAAG | 4 cut(s) | 244, 385, 830, 1158 |
| Eco91I | GGTNACC | 1 cut(s) | 914 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 458 |
| EcoO65I | GGTNACC | 1 cut(s) | 914 |
| EcoRI | GAATTC | 1 cut(s) | 1815 |
| EcoRII | CCWGG | 2 cut(s) | 694, 1380 |
| EcoT14I | CCWWGG | 4 cut(s) | 1110, 1202, 1690, 1953 |
| EcoT22I | ATGCAT | 2 cut(s) | 1009, 1933 |
| ErhI | CCWWGG | 4 cut(s) | 1110, 1202, 1690, 1953 |
| FaqI | GGGAC | 2 cut(s) | 122, 1228 |
| FbaI | TGATCA | 2 cut(s) | 648, 1321 |
| FblI | GTMKAC | 1 cut(s) | 788 |
| Fnu4HI | GCNGC | 6 cut(s) | 1029, 1116, 1451, 1587, 1647, 1665 |
| FokI | GGATG | 4 cut(s) | 262, 413, 819, 1202 |
| Fsp4HI | GCNGC | 6 cut(s) | 1029, 1116, 1451, 1587, 1647, 1665 |
| FspBI | CTAG | 4 cut(s) | 72, 719, 827, 1911 |
| GlaI | GCGC | 1 cut(s) | 504 |
| GluI | GCNGC | 6 cut(s) | 1029, 1116, 1451, 1587, 1647, 1665 |
| GsaI | CCCAGC | 1 cut(s) | 1227 |
| GsuI | CTGGAG | 3 cut(s) | 311, 1533, 1773 |
| HaeIII | GGCC | 1 cut(s) | 41 |
| HapII | CCGG | 5 cut(s) | 98, 669, 743, 750, 1901 |
| HgaI | GACGC | 2 cut(s) | 403, 1072 |
| HhaI | GCGC | 1 cut(s) | 505 |
| Hin1I | GRCGYC | 3 cut(s) | 5, 414, 1064 |
| Hin6I | GCGC | 1 cut(s) | 503 |
| HinP1I | GCGC | 1 cut(s) | 503 |
| HincII | GTYRAC | 1 cut(s) | 1521 |
| HindII | GTYRAC | 1 cut(s) | 1521 |
| HindIII | AAGCTT | 2 cut(s) | 1373, 1410 |
| HinfI | GANTC | 6 cut(s) | 264, 659, 938, 1162, 1457, 1793 |
| HpaI | GTTAAC | 1 cut(s) | 1521 |
| HpaII | CCGG | 5 cut(s) | 98, 669, 743, 750, 1901 |
| HphI | GGTGA | 7 cut(s) | 141, 523, 619, 764, 926, 1316, 1615 |
| Hpy166II | GTNNAC | 6 cut(s) | 567, 777, 789, 1259, 1521, 1687 |
| Hpy188I | TCNGA | 5 cut(s) | 263, 477, 658, 703, 1830 |
| Hpy188III | TCNNGA | 7 cut(s) | 328, 422, 719, 884, 1435, 1547, 1790 |
| Hpy8I | GTNNAC | 6 cut(s) | 567, 777, 789, 1259, 1521, 1687 |
| Hpy99I | CGWCG | 3 cut(s) | 10, 233, 1066 |
| HpyAV | CCTTC | 9 cut(s) | 64, 68, 121, 156, 409, 651, 706, 1238, 1952 |
| HpyCH4III | ACNGT | 3 cut(s) | 133, 731, 880 |
| HpyCH4IV | ACGT | 2 cut(s) | 231, 1713 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 314, 1115, 1124, 1133, 1571 |
| HpyF3I | CTNAG | 4 cut(s) | 44, 347, 1360, 1845 |
| HpySE526I | ACGT | 2 cut(s) | 231, 1713 |
| Hsp92I | GRCGYC | 3 cut(s) | 5, 414, 1064 |
| HspAI | GCGC | 1 cut(s) | 503 |
| Ksp22I | TGATCA | 2 cut(s) | 648, 1321 |
| KspAI | GTTAAC | 1 cut(s) | 1521 |
| Kzo9I | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| LmnI | GCTCC | 4 cut(s) | 100, 1277, 1405, 1789 |
| Lsp1109I | GCAGC | 4 cut(s) | 1462, 1573, 1658, 1676 |
| LweI | GCATC | 6 cut(s) | 360, 1016, 1180, 1643, 1918, 1969 |
| MaeI | CTAG | 4 cut(s) | 72, 719, 827, 1911 |
| MaeII | ACGT | 2 cut(s) | 231, 1713 |
| MaeIII | GTNAC | 7 cut(s) | 404, 415, 874, 914, 970, 1264, 1921 |
| MalI | GATC | 7 cut(s) | 650, 1022, 1323, 1488, 1545, 1736, 2051 |
| MbiI | CCGCTC | 1 cut(s) | 1031 |
| MboI | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| MflI | RGATCY | 1 cut(s) | 2049 |
| MhlI | GDGCHC | 2 cut(s) | 1556, 1689 |
| MluCI | AATT | 5 cut(s) | 890, 1495, 1526, 1815, 1999 |
| MlyI | GAGTC | 2 cut(s) | 932, 1802 |
| MmeI | TCCRAC | 3 cut(s) | 294, 320, 1190 |
| Mph1103I | ATGCAT | 2 cut(s) | 1009, 1933 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1232 |
| MseI | TTAA | 6 cut(s) | 12, 987, 1173, 1394, 1442, 1520 |
| MspA1I | CMGCKG | 2 cut(s) | 341, 1471 |
| MspI | CCGG | 5 cut(s) | 98, 669, 743, 750, 1901 |
| MspR9I | CCNGG | 3 cut(s) | 696, 743, 1382 |
| Mva1269I | GAATGC | 3 cut(s) | 979, 1501, 1576 |
| MvaI | CCWGG | 2 cut(s) | 696, 1382 |
| MvnI | CGCG | 1 cut(s) | 503 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 314, 1115, 1124, 1133, 1571 |
| NciI | CCSGG | 1 cut(s) | 743 |
| NcoI | CCATGG | 1 cut(s) | 1202 |
| NdeII | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| NheI | GCTAGC | 1 cut(s) | 826 |
| NlaIV | GGNNCC | 1 cut(s) | 2051 |
| NmeAIII | GCCGAG | 2 cut(s) | 390, 1562 |
| NmuCI | GTSAC | 5 cut(s) | 404, 415, 874, 914, 970 |
| NsiI | ATGCAT | 2 cut(s) | 1009, 1933 |
| NspI | RCATGY | 3 cut(s) | 483, 1685, 1705 |
| NspV | TTCGAA | 1 cut(s) | 1037 |
| PagI | TCATGA | 1 cut(s) | 1434 |
| PciI | ACATGT | 1 cut(s) | 1701 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 614 |
| PctI | GAATGC | 3 cut(s) | 979, 1501, 1576 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1232 |
| PfeI | GAWTC | 4 cut(s) | 264, 659, 1162, 1457 |
| PkrI | GCNGC | 6 cut(s) | 1030, 1117, 1452, 1588, 1648, 1666 |
| PleI | GAGTC | 2 cut(s) | 932, 1801 |
| PpsI | GAGTC | 2 cut(s) | 932, 1801 |
| PscI | ACATGT | 1 cut(s) | 1701 |
| PshBI | ATTAAT | 1 cut(s) | 987 |
| PsiI | TTATAA | 1 cut(s) | 677 |
| Psp6I | CCWGG | 2 cut(s) | 694, 1380 |
| PspEI | GGTNACC | 1 cut(s) | 914 |
| PspFI | CCCAGC | 1 cut(s) | 1223 |
| PspGI | CCWGG | 2 cut(s) | 694, 1380 |
| PspN4I | GGNNCC | 1 cut(s) | 2051 |
| PspPI | GGNCC | 1 cut(s) | 40 |
| PstNI | CAGNNNCTG | 1 cut(s) | 701 |
| PsuI | RGATCY | 1 cut(s) | 2049 |
| PvuII | CAGCTG | 1 cut(s) | 341 |
| RsaI | GTAC | 5 cut(s) | 216, 324, 566, 605, 899 |
| RsaNI | GTAC | 5 cut(s) | 215, 323, 565, 604, 898 |
| SaqAI | TTAA | 6 cut(s) | 12, 987, 1173, 1394, 1442, 1520 |
| SatI | GCNGC | 6 cut(s) | 1029, 1116, 1451, 1587, 1647, 1665 |
| Sau3AI | GATC | 7 cut(s) | 648, 1020, 1321, 1486, 1543, 1734, 2049 |
| Sau96I | GGNCC | 1 cut(s) | 40 |
| SchI | GAGTC | 2 cut(s) | 932, 1802 |
| ScrFI | CCNGG | 3 cut(s) | 696, 743, 1382 |
| SduI | GDGCHC | 2 cut(s) | 1556, 1689 |
| SfaNI | GCATC | 6 cut(s) | 360, 1016, 1180, 1643, 1918, 1969 |
| SfcI | CTRYAG | 1 cut(s) | 253 |
| SfuI | TTCGAA | 1 cut(s) | 1037 |
| Sse9I | AATT | 5 cut(s) | 890, 1495, 1526, 1815, 1999 |
| SspI | AATATT | 1 cut(s) | 242 |
| SspMI | CTAG | 4 cut(s) | 72, 719, 827, 1911 |
| StyD4I | CCNGG | 3 cut(s) | 694, 741, 1380 |
| StyI | CCWWGG | 4 cut(s) | 1110, 1202, 1690, 1953 |
| TaaI | ACNGT | 3 cut(s) | 133, 731, 880 |
| TaiI | ACGT | 2 cut(s) | 234, 1716 |
| TaqI | TCGA | 2 cut(s) | 1037, 1061 |
| TasI | AATT | 5 cut(s) | 890, 1495, 1526, 1815, 1999 |
| TatI | WGTACW | 4 cut(s) | 214, 564, 603, 897 |
| TauI | GCSGC | 2 cut(s) | 1031, 1118 |
| TfiI | GAWTC | 4 cut(s) | 264, 659, 1162, 1457 |
| Tru1I | TTAA | 6 cut(s) | 12, 987, 1173, 1394, 1442, 1520 |
| Tru9I | TTAA | 6 cut(s) | 12, 987, 1173, 1394, 1442, 1520 |
| TscAI | CASTG | 8 cut(s) | 409, 651, 883, 1287, 1368, 1572, 1774, 1849 |
| TseFI | GTSAC | 5 cut(s) | 404, 415, 874, 914, 970 |
| TseI | GCWGC | 4 cut(s) | 1450, 1586, 1646, 1664 |
| Tsp45I | GTSAC | 5 cut(s) | 404, 415, 874, 914, 970 |
| TspDTI | ATGAA | 9 cut(s) | 16, 22, 651, 830, 1074, 1257, 1423, 1451, 1494 |
| TspGWI | ACGGA | 1 cut(s) | 310 |
| TspRI | CASTG | 8 cut(s) | 409, 651, 883, 1287, 1368, 1572, 1774, 1849 |
| VneI | GTGCAC | 1 cut(s) | 1685 |
| VspI | ATTAAT | 1 cut(s) | 987 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 458 |
| XapI | RAATTY | 3 cut(s) | 1526, 1815, 1999 |
| XbaI | TCTAGA | 1 cut(s) | 718 |
| XceI | RCATGY | 3 cut(s) | 483, 1685, 1705 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1334 |
| XmiI | GTMKAC | 1 cut(s) | 788 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1232 |
| XspI | CTAG | 4 cut(s) | 72, 719, 827, 1911 |
| Zsp2I | ATGCAT | 2 cut(s) | 1009, 1933 |