Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000403

Physical Location & Seq

Genomic Coordinates Forward (+)

5841334 .. 5843547

Gene Structure 2214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1072700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2214 bp

ATGTTGAAAAAAGTGAGAAGAAAAGTCAAAACCTTAATCACCAAGAGTTTCAAAAAACGCAAGAAAGATCCCTGTAAACCGGCACCCCCTCCTCCATCTTCATTTCCATCACCACCCCCTCCCGAAGAACCCCAAACTATGTCGCCACCGCCGCAGCAAACTCTCAGGCCACCCGCCACCGTCGGCCCATTCCTTTTCCCCCAAACCCAATCCACCGTGCTCCCCGACCCTTCCAACTTCTTCTCCCCAAACCTCCTCTCCTCCCCTCTCCCCACCAACTCCTTCTTCCAAAACTTCGCCCTCAAAAACGGCGATCAACCCGAGTACTTCCACCCCTACGCCGTTAAATCCTCCTCCGCCGCCCTAACCATCTGCTACCCATCTCGCTTCTCCACCTCCGCCTTCATATATCAAGTCTTCATTGCCGATCTCGCCATCTCCGCGGCCCAAATGCCCGATTCCCCACAACCCCACAAAATCTCCTCCTTCAACGACCTCAGCATCACATTGGATTTCCCCTCCTCCAACCTCCACTTCTTCCTCGTCCGCGGCAGCCCATTCATCACCTGCTCTGTTTCCAACTCCACCTCCCTTTCAATCTCCACCATCCACGCAATCCTCTCGTGTGAATCAAACGGCTCAAAGACCAAATACACCATCAAGCTCAACAGCAACCAGACATGGCTCGTATACACTTCCTCCCCGATCGATTTGGCAACCAGCGGCCTTTCATTGATCACCTCCAATGGGTTTTCTGGGATTGTTCGGATTGCGATATTGCCGGATTCGAACCCGAAATTCGAGGCGAGCCTTGACCGGTTCAGTTCTTGCTACCCGGTTTCCGGAGAAGCGGTTTTCACAAAGCCGTTTGTTTTGGAGTACAGATGGGAGAAGAAAGGATGGGGCGATTTGCTTATGCTTGCTCATCCTCTGCACCTCCAGCTTCTCTCCGGCGAGGATTCTCACGTCACTGTTTTGGAGGATTTCAAGTTCAAGAGCATTGATGGGGACCTTGTTGGTGTTGTTGGCGATTCATGGGCGCTGAAACCCGACCCTGTTTCGATCACCTGGCATTCGAGTCAAGGAGTTAGGGAACATTCGTATGAGGAAATTGTTTCTGCGCTTGTTAAAGATGTTGGGGATCTGAATTCGAGTGGGATAGCTACAATGTCTTCGTATTTTTATGGGAAATTGATCGCGAGGGCGGCGAGGCTGGCTTTGATTGCTGAGGAGGTGGCTTATCTGGATGTGATTCCGGCGATTAGGAAGTTCTTGAAGGAAACGATTGAGCCGTGGTTGGACGGAACTTTCCCGGGAAATGGTTTCTTGTATGATAAGATTTGGGGTGGCATTGTCACGAAACAAGGGGCAACTGATTCGGGTGCAGATTTCGGGTTTGGAGTGTACAATGATCACCATTATCATCTGGGCTACTTTGTTTATGGGATTTCAGTGCTTGCGAAAATTGATCCTGCTTGGGGAAGGAAGTATCGGGCTAAAGCGTATTCGCTCGCTTTTGATTATATGACGGTAGGCAGGCGATCACATTCGCATTATCCGCGCTTGAGGTGCTTTGATTTTTACAAATTGCACTCGTGGGCAGGCGGGTTAACTGAGTTTGCGGATGGTCGTAATCAGGAGAGCACGAGTGAGGCAGTGAATGCCTACTATTCAGCTGCTTTGTTGGGATTAGCTTATGGAGACACGCATCTTGTGGCCACTGGATCACTGCTTGCAGCTTTGGAGATTCAGGCGGCGCAAATGTGGTGGCACGTAAAAGAGGGAGGTAACATGTACGAGGAGGATTTCACAAGGGAAAATCGTGTGGTTGGAATGGTATGGAATAACAAGAGGGACAGCGGACTTTGGTTCGCTCCCCCGGAGTGGAAAGAGTGCAGGCTTGGAATTCAGTTGCTACCGCTATTGCCGATCACCGAGGCCTTGTTTTCAGATGTTGGCTTTGTTAAGGATCTTGTGAAATGGACACTACCGGCTTTGAGTAGGGAGGGAGTTGGGGAAGGATGGAAAGGATTTGTCTATTCCTTGGAAGGGATTTACGACAAAGAACTCGCTTTGGGGAAGATAAGGAATTTGAATGGTTACGATGATGGTAACTCGCTTACTAATCTTCTGTGGTGGATCCACAGCAGAGGAGAGGCAGGAGCGGAATTCGGACTTGGACACAACGTTTGTTGGTCTAGGCACTATCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0000746 GO:0000747 GO:0000920 GO:0000935 GO:0000936 GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005937 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006076 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0009986 GO:0009987 GO:0010383 GO:0015629 GO:0015926 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016998 GO:0019953 GO:0022411 GO:0022414 GO:0030246 GO:0030247 GO:0030427 GO:0030428 GO:0030994 GO:0031505 GO:0032153 GO:0032155 GO:0042973 GO:0042995 GO:0043170 GO:0043187 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043332 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044277 GO:0044347 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044457 GO:0044463 GO:0044464 GO:0044703 GO:0044764 GO:0045229 GO:0051273 GO:0051275 GO:0051286 GO:0051301 GO:0051704 GO:0070871 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0071853 GO:0120025 GO:0120038 GO:1901575 GO:1904541 GO:1990819

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_81

Protein Analysis

738

Amino Acids

81.95

Weight (kDa)

6.88

Isoelectric Point (pI)

43.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_81	PF03639	86 - 357	3.9e-64	Glycosyl hydrolase family 81 N-terminal domain
Glyco_hydro81C	PF17652	364 - 714	6.4e-104	Glycosyl hydrolase family 81 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18310 AT5G15870
fragaria_vesca	FvH4_3g44967 FvH4_6g45580 FvH4_6g47860 FvH4_6g47870 FvH4_6g53160
malus_domestica	MD09G1047100.v1.1 MD09G1081400.v1.1 MD09G1081500.v1.1 MD09G1081600.v1.1 MD09G1081700.v1.1 MD15G1128600.v1.1 MD17G1047200.v1.1 MD17G1048700.v1.1 MD17G1071200.v1.1 MD17G1072700.v1.1
prunus_persica	Prupe.3G242000_v2.0.a1 Prupe.3G242100_v2.0.a1 Prupe.3G261700_v2.0.a1 Prupe.8G191700_v2.0.a1 Prupe.8G194600_v2.0.a1 Prupe.8G259500_v2.0.a1
pyrus_communis	pycom09g00810 pycom09g00820 pycom09g00830 pycom15g11570 pycom17g04350 pycom17g07070 pycom17g07350
rosa_chinensis	RchiOBHm_Chr1g0366281 RchiOBHm_Chr2g0163531 RchiOBHm_Chr2g0163581 RchiOBHm_Chr2g0167061 RchiOBHm_Chr2g0167071 RchiOBHm_Chr3g0490821 RchiOBHm_Chr3g0490841 RchiOBHm_Chr3g0492161 RchiOBHm_Chr3g0492171
rosa_laevigata	RLG00000021408 RLG00000021693 RLG00000021694 RLG00000022819 RLG00000022827
rosa_multiflora	Rmu_co8304887.1_g000001 Rmu_co8334235.1_g000001 Rmu_co8488335.1_g000001 Rmu_sc0003484.1_g000001 Rmu_sc0003484.1_g000004 Rmu_sc0010966.1_g000013 Rmu_sc0012462.1_g000001 Rmu_sc0019504.1_g000002 Rmu_sc0019504.1_g000004 Rmu_sc0022540.1_g000001 Rmu_sc0023760.1_g000001 Rmu_sc0038280.1_g000002
rosa_roxburghii	Rroxscaffold_2G00077500 Rroxscaffold_2G00083750 Rroxscaffold_2G00083760 Rroxscaffold_2G00087160 Rroxscaffold_2G00132530 Rroxscaffold_4G00290030 Rroxscaffold_4G00290040 Rroxscaffold_4G00290280
rosa_rugosa	Rorug01G0328400 Rorug02G0265200 Rorug02G0265900 Rorug02G0266000 Rorug02G0266800.1 Rorug02G0266900.1 Rorug02G0267500 Rorug02G0267600 Rorug02G0268300 Rorug02G0268800 Rorug02G0268900 Rorug02G0503600 Rorug02G0528200 Rorug02G0528300 Rorug03G0247200 Rorug03G0247300
rosa_samantha	Rh1BG298400 Rh1DG329800 Rh2AG570500 Rh2AG570700 Rh2AG595000 Rh2AG595100 Rh2AG595200 Rh2CG552500 Rh2CG552700 Rh2CG577100 Rh2CG577200 Rh2DG592500 Rh2DG617800 Rh2DG617900 Rh3CG333000 Rh3DG335000
rosa_wichuraiana	Rw2G047230 Rw2G049530 Rw2G049540 Rw3G026510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	575
Acc36I	ACCTGC	1 cut(s)	575
AccB1I	GGYRCC	1 cut(s)	82
AccBSI	CCGCTC	1 cut(s)	2165
AccI	GTMKAC	1 cut(s)	690
AccII	CGCG	4 cut(s)	443, 549, 1199, 1561
AccIII	TCCGGA	1 cut(s)	842
AclI	AACGTT	1 cut(s)	2187
AclWI	GGATC	7 cut(s)	62, 1149, 1463, 1732, 1977, 2134, 2147
AcoI	YGGCCR	1 cut(s)	1716
AcsI	RAATTY	5 cut(s)	797, 1147, 1905, 2089, 2168
AfaI	GTAC	4 cut(s)	326, 881, 1406, 1796
AfiI	CCNNNNNNNGG	6 cut(s)	747, 842, 1319, 1478, 1884, 2049
AflIII	ACRYGT	1 cut(s)	1791
AgeI	ACCGGT	1 cut(s)	816
AgsI	TTSAA	8 cut(s)	7, 52, 490, 597, 988, 994, 1276, 2095
AjiI	CACGTC	1 cut(s)	967
AjnI	CCWGG	1 cut(s)	1067
AjuI	GAANNNNNNNTTGG	6 cut(s)	269, 301, 518, 550, 2160, 2192
AloI	GAACNNNNNNTCC	2 cut(s)	974, 1006
AluBI	AGCT	6 cut(s)	664, 943, 1163, 1676, 1694, 1739
AluI	AGCT	6 cut(s)	664, 943, 1163, 1676, 1694, 1739
Alw21I	GWGCWC	2 cut(s)	222, 1646
Alw26I	GTCTC	1 cut(s)	1695
AlwI	GGATC	7 cut(s)	62, 1149, 1463, 1732, 1977, 2134, 2147
Ama87I	CYCGRG	2 cut(s)	320, 1312
Aor13HI	TCCGGA	1 cut(s)	842
AoxI	GGCC	6 cut(s)	167, 184, 444, 724, 1716, 1938
ApeKI	GCWGC	4 cut(s)	154, 552, 1676, 1736
ApoI	RAATTY	5 cut(s)	797, 1147, 1905, 2089, 2168
ArsI	GACNNNNNNTTYG	2 cut(s)	2172, 2204
AsiGI	ACCGGT	1 cut(s)	816
AspLEI	GCGC	4 cut(s)	1042, 1123, 1563, 1759
AspS9I	GGNCC	3 cut(s)	185, 445, 1009
AsuC2I	CCSGG	4 cut(s)	836, 1313, 1314, 1880
AsuHPI	GGTGA	7 cut(s)	31, 102, 556, 730, 1057, 1406, 1924
AsuII	TTCGAA	1 cut(s)	788
AvaI	CYCGRG	2 cut(s)	320, 1312
AvaII	GGWCC	1 cut(s)	1009
BalI	TGGCCA	1 cut(s)	1718
BamHI	GGATCC	1 cut(s)	2139
BanI	GGYRCC	1 cut(s)	82
BauI	CACGAG	3 cut(s)	622, 1594, 1645
BbsI	GAAGAC	2 cut(s)	409, 1164
Bbv12I	GWGCWC	2 cut(s)	222, 1646
BbvCI	CCTCAGC	2 cut(s)	497, 1227
BbvI	GCAGC	4 cut(s)	166, 564, 1663, 1748
BceAI	ACGGC	5 cut(s)	325, 326, 652, 850, 1276
BcgI	CGANNNNNNTGC	4 cut(s)	603, 637, 1359, 1393
BciT130I	CCWGG	1 cut(s)	1069
BclI	TGATCA	2 cut(s)	735, 1411
BcnI	CCSGG	4 cut(s)	836, 1313, 1314, 1880
BcoDI	GTCTC	1 cut(s)	1695
BfaI	CTAG	1 cut(s)	2199
BfoI	RGCGCY	1 cut(s)	1043
BfuAI	ACCTGC	1 cut(s)	575
BmcAI	AGTACT	1 cut(s)	326
Bme1390I	CCNGG	5 cut(s)	836, 1069, 1313, 1314, 1880
Bme18I	GGWCC	1 cut(s)	1009
BmeT110I	CYCGRG	2 cut(s)	320, 1312
BmgBI	CACGTC	1 cut(s)	967
BmgT120I	GGNCC	3 cut(s)	185, 445, 1009
BmiI	GGNNCC	3 cut(s)	84, 1010, 2141
BmrFI	CCNGG	5 cut(s)	836, 1069, 1313, 1314, 1880
BmsI	GCATC	2 cut(s)	510, 1717
BpiI	GAAGAC	2 cut(s)	409, 1164
BpmI	CTGGAG	1 cut(s)	923
Bpu10I	CCTNAGC	2 cut(s)	497, 1227
Bpu14I	TTCGAA	1 cut(s)	788
BpuEI	CTTGAG	1 cut(s)	1585
BpuMI	CCSGG	4 cut(s)	836, 1313, 1314, 1880
Bsa29I	ATCGAT	1 cut(s)	708
BsaAI	YACGTR	1 cut(s)	1774
BsaJI	CCNNGG	7 cut(s)	441, 547, 1292, 1312, 1878, 1935, 2043
BsaWI	WCCGGW	2 cut(s)	816, 842
Bsc4I	CCNNNNNNNGG	6 cut(s)	747, 842, 1319, 1478, 1884, 2049
Bse118I	RCCGGY	3 cut(s)	79, 816, 1990
Bse1I	ACTGG	1 cut(s)	1726
Bse3DI	GCAATG	1 cut(s)	420
BseAI	TCCGGA	1 cut(s)	842
BseBI	CCWGG	1 cut(s)	1069
BseCI	ATCGAT	1 cut(s)	708
BseDI	CCNNGG	7 cut(s)	441, 547, 1292, 1312, 1878, 1935, 2043
BseGI	GGATG	6 cut(s)	606, 905, 925, 1252, 1630, 2027
BseLI	CCNNNNNNNGG	6 cut(s)	747, 842, 1319, 1478, 1884, 2049
BseMI	GCAATG	1 cut(s)	420
BseMII	CTCAG	4 cut(s)	178, 511, 1218, 1605
BseNI	ACTGG	1 cut(s)	1726
BseRI	GAGGAG	9 cut(s)	81, 245, 250, 343, 472, 511, 1244, 1814, 2166
BseXI	GCAGC	4 cut(s)	166, 564, 1663, 1748
BsgI	GTGCAG	3 cut(s)	917, 1404, 1915
Bsh1236I	CGCG	4 cut(s)	443, 549, 1199, 1561
Bsh1285I	CGRYCG	1 cut(s)	708
BshFI	GGCC	6 cut(s)	169, 186, 446, 726, 1718, 1940
BshNI	GGYRCC	1 cut(s)	82
BshTI	ACCGGT	1 cut(s)	816
BshVI	ATCGAT	1 cut(s)	708
BsiEI	CGRYCG	1 cut(s)	708
BsiHKAI	GWGCWC	2 cut(s)	222, 1646
BsiHKCI	CYCGRG	2 cut(s)	320, 1312
BslFI	GGGAC	2 cut(s)	1022, 1868
BslI	CCNNNNNNNGG	6 cut(s)	747, 842, 1319, 1478, 1884, 2049
BsmAI	GTCTC	1 cut(s)	1695
BsmFI	GGGAC	2 cut(s)	1022, 1868
BsmI	GAATGC	2 cut(s)	1072, 1666
BsnI	GGCC	6 cut(s)	169, 186, 446, 726, 1718, 1940
BsoBI	CYCGRG	2 cut(s)	320, 1312
Bsp119I	TTCGAA	1 cut(s)	788
Bsp1286I	GDGCHC	2 cut(s)	222, 1646
Bsp13I	TCCGGA	1 cut(s)	842
Bsp1407I	TGTACA	1 cut(s)	1404
Bsp68I	TCGCGA	1 cut(s)	1199
BspANI	GGCC	6 cut(s)	169, 186, 446, 726, 1718, 1940
BspCNI	CTCAG	4 cut(s)	177, 510, 1219, 1606
BspDI	ATCGAT	1 cut(s)	708
BspEI	TCCGGA	1 cut(s)	842
BspFNI	CGCG	4 cut(s)	443, 549, 1199, 1561
BspLI	GGNNCC	3 cut(s)	84, 1010, 2141
BspMI	ACCTGC	1 cut(s)	575
BspPI	GGATC	7 cut(s)	62, 1149, 1463, 1732, 1977, 2134, 2147
BspT104I	TTCGAA	1 cut(s)	788
BspT107I	GGYRCC	1 cut(s)	82
BsrBI	CCGCTC	1 cut(s)	2165
BsrDI	GCAATG	1 cut(s)	420
BsrFI	RCCGGY	3 cut(s)	79, 816, 1990
BsrGI	TGTACA	1 cut(s)	1404
BsrI	ACTGG	1 cut(s)	1726
BssAI	RCCGGY	3 cut(s)	79, 816, 1990
BssECI	CCNNGG	7 cut(s)	441, 547, 1292, 1312, 1878, 1935, 2043
BssNAI	GTATAC	1 cut(s)	691
BssSI	CACGAG	3 cut(s)	622, 1594, 1645
BssT1I	CCWWGG	1 cut(s)	2043
Bst1107I	GTATAC	1 cut(s)	691
Bst2BI	CACGAG	3 cut(s)	622, 1594, 1645
Bst2UI	CCWGG	1 cut(s)	1069
Bst4CI	ACNGT	4 cut(s)	181, 217, 973, 1531
BstAPI	GCANNNNNTGC	1 cut(s)	1661
BstAUI	TGTACA	1 cut(s)	1404
BstBAI	YACGTR	1 cut(s)	1774
BstBI	TTCGAA	1 cut(s)	788
BstC8I	GCNNGC	9 cut(s)	808, 921, 1215, 1458, 1512, 1538, 1603, 1734, 1898
BstDEI	CTNAG	4 cut(s)	164, 497, 1227, 1614
BstDSI	CCRYGG	3 cut(s)	441, 547, 1292
BstF5I	GGATG	6 cut(s)	606, 905, 925, 1252, 1630, 2027
BstFNI	CGCG	4 cut(s)	443, 549, 1199, 1561
BstH2I	RGCGCY	1 cut(s)	1043
BstHHI	GCGC	4 cut(s)	1042, 1123, 1563, 1759
BstMAI	GTCTC	1 cut(s)	1695
BstMCI	CGRYCG	1 cut(s)	708
BstNI	CCWGG	1 cut(s)	1069
BstNSI	RCATGY	1 cut(s)	1795
BstSCI	CCNGG	5 cut(s)	834, 1067, 1311, 1312, 1878
BstUI	CGCG	4 cut(s)	443, 549, 1199, 1561
BstV1I	GCAGC	4 cut(s)	166, 564, 1663, 1748
BstV2I	GAAGAC	2 cut(s)	409, 1164
BstX2I	RGATCY	4 cut(s)	67, 1141, 1969, 2139
BstYI	RGATCY	4 cut(s)	67, 1141, 1969, 2139
BstZ17I	GTATAC	1 cut(s)	691
Bsu15I	ATCGAT	1 cut(s)	708
BsuRI	GGCC	6 cut(s)	169, 186, 446, 726, 1718, 1940
BsuTUI	ATCGAT	1 cut(s)	708
BtgI	CCRYGG	3 cut(s)	441, 547, 1292
BtrI	CACGTC	1 cut(s)	967
BtsCI	GGATG	6 cut(s)	606, 905, 925, 1252, 1630, 2027
BtsI	GCAGTG	2 cut(s)	1662, 1727
BtsIMutI	CAGTG	5 cut(s)	969, 1458, 1662, 1719, 1727
BtuMI	TCGCGA	1 cut(s)	1199
BveI	ACCTGC	1 cut(s)	575
Cac8I	GCNNGC	9 cut(s)	808, 921, 1215, 1458, 1512, 1538, 1603, 1734, 1898
CfoI	GCGC	4 cut(s)	1042, 1123, 1563, 1759
Cfr10I	RCCGGY	3 cut(s)	79, 816, 1990
Cfr13I	GGNCC	3 cut(s)	185, 445, 1009
Cfr42I	CCGCGG	2 cut(s)	444, 550
Cfr9I	CCCGGG	1 cut(s)	1312
ClaI	ATCGAT	1 cut(s)	708
Csp6I	GTAC	4 cut(s)	325, 880, 1405, 1795
CspAI	ACCGGT	1 cut(s)	816
CspCI	CAANNNNNGTGG	2 cut(s)	1748, 1783
CviAII	CATG	3 cut(s)	681, 1035, 1792
CviQI	GTAC	4 cut(s)	325, 880, 1405, 1795
DdeI	CTNAG	4 cut(s)	164, 497, 1227, 1614
EaeI	YGGCCR	1 cut(s)	1716
EciI	GGCGGA	2 cut(s)	346, 388
Eco130I	CCWWGG	1 cut(s)	2043
Eco147I	AGGCCT	1 cut(s)	1940
Eco47I	GGWCC	1 cut(s)	1009
Eco88I	CYCGRG	2 cut(s)	320, 1312
EcoO109I	RGGNCCY	1 cut(s)	1009
EcoRI	GAATTC	3 cut(s)	1147, 1905, 2168
EcoRII	CCWGG	1 cut(s)	1067
EcoT14I	CCWWGG	1 cut(s)	2043
ErhI	CCWWGG	1 cut(s)	2043
FaeI	CATG	3 cut(s)	684, 1038, 1795
FaqI	GGGAC	2 cut(s)	1022, 1868
FatI	CATG	3 cut(s)	680, 1034, 1791
FauI	CCCGC	2 cut(s)	181, 1598
FbaI	TGATCA	2 cut(s)	735, 1411
FblI	GTMKAC	1 cut(s)	690
FokI	GGATG	6 cut(s)	593, 912, 912, 1259, 1637, 2034
FspBI	CTAG	1 cut(s)	2199
GlaI	GCGC	4 cut(s)	1041, 1122, 1562, 1758
GsuI	CTGGAG	1 cut(s)	923
HaeII	RGCGCY	1 cut(s)	1043
HaeIII	GGCC	6 cut(s)	169, 186, 446, 726, 1718, 1940
HhaI	GCGC	4 cut(s)	1042, 1123, 1563, 1759
Hin1II	CATG	3 cut(s)	684, 1038, 1795
Hin6I	GCGC	4 cut(s)	1040, 1121, 1561, 1757
HinP1I	GCGC	4 cut(s)	1040, 1121, 1561, 1757
HincII	GTYRAC	1 cut(s)	1611
HindII	GTYRAC	1 cut(s)	1611
HinfI	GANTC	9 cut(s)	458, 629, 785, 959, 1031, 1078, 1252, 1376, 1747
HpaI	GTTAAC	1 cut(s)	1611
HphI	GGTGA	7 cut(s)	31, 102, 556, 730, 1057, 1406, 1924
Hpy166II	GTNNAC	4 cut(s)	77, 691, 1405, 1611
Hpy188I	TCNGA	4 cut(s)	768, 1146, 1951, 2174
Hpy188III	TCNNGA	8 cut(s)	122, 843, 994, 1198, 1244, 1273, 1357, 1637
Hpy8I	GTNNAC	4 cut(s)	77, 691, 1405, 1611
Hpy99I	CGWCG	1 cut(s)	185
HpyAV	CCTTC	8 cut(s)	240, 292, 412, 496, 1270, 1476, 2012, 2042
HpyCH4III	ACNGT	4 cut(s)	181, 217, 973, 1531
HpyCH4IV	ACGT	3 cut(s)	966, 1773, 2187
HpyCH4V	TGCA	5 cut(s)	934, 1385, 1591, 1736, 1896
HpyF3I	CTNAG	4 cut(s)	164, 497, 1227, 1614
HpySE526I	ACGT	3 cut(s)	966, 1773, 2187
Hsp92II	CATG	3 cut(s)	684, 1038, 1795
HspAI	GCGC	4 cut(s)	1040, 1121, 1561, 1757
Kpn2I	TCCGGA	1 cut(s)	842
Ksp22I	TGATCA	2 cut(s)	735, 1411
KspAI	GTTAAC	1 cut(s)	1611
KspI	CCGCGG	2 cut(s)	444, 550
LmnI	GCTCC	3 cut(s)	225, 1879, 2162
Lsp1109I	GCAGC	4 cut(s)	166, 564, 1663, 1748
LweI	GCATC	2 cut(s)	510, 1717
MaeI	CTAG	1 cut(s)	2199
MaeII	ACGT	3 cut(s)	966, 1773, 2187
MaeIII	GTNAC	5 cut(s)	967, 1354, 1787, 2099, 2111
MbiI	CCGCTC	1 cut(s)	2165
MflI	RGATCY	4 cut(s)	67, 1141, 1969, 2139
MhlI	GDGCHC	2 cut(s)	222, 1646
MlsI	TGGCCA	1 cut(s)	1718
MluCI	AATT	9 cut(s)	797, 1110, 1147, 1190, 1464, 1586, 1905, 2089, 2168
MluNI	TGGCCA	1 cut(s)	1718
MlyI	GAGTC	1 cut(s)	1087
MmeI	TCCRAC	5 cut(s)	258, 549, 603, 1278, 1810
Mox20I	TGGCCA	1 cut(s)	1718
MroI	TCCGGA	1 cut(s)	842
MscI	TGGCCA	1 cut(s)	1718
MseI	TTAA	6 cut(s)	35, 345, 1128, 1610, 1965, 2212
MslI	CAYNNNNRTG	1 cut(s)	1101
Msp20I	TGGCCA	1 cut(s)	1718
MspA1I	CMGCKG	5 cut(s)	443, 549, 723, 1676, 1860
MspR9I	CCNGG	5 cut(s)	836, 1069, 1313, 1314, 1880
Mva1269I	GAATGC	2 cut(s)	1072, 1666
MvaI	CCWGG	1 cut(s)	1069
MvnI	CGCG	4 cut(s)	443, 549, 1199, 1561
NciI	CCSGG	4 cut(s)	836, 1313, 1314, 1880
NlaIII	CATG	3 cut(s)	684, 1038, 1795
NlaIV	GGNNCC	3 cut(s)	84, 1010, 2141
NmuCI	GTSAC	2 cut(s)	967, 1354
NruI	TCGCGA	1 cut(s)	1199
NspI	RCATGY	1 cut(s)	1795
NspV	TTCGAA	1 cut(s)	788
PaqCI	CACCTGC	1 cut(s)	575
PceI	AGGCCT	1 cut(s)	1940
PciI	ACATGT	1 cut(s)	1791
PcsI	WCGNNNNNNNCGW	1 cut(s)	1289
PctI	GAATGC	2 cut(s)	1072, 1666
PfeI	GAWTC	8 cut(s)	458, 629, 785, 959, 1031, 1252, 1376, 1747
PinAI	ACCGGT	1 cut(s)	816
Ple19I	CGATCG	1 cut(s)	708
PleI	GAGTC	1 cut(s)	1086
PpsI	GAGTC	1 cut(s)	1086
Ppu21I	YACGTR	1 cut(s)	1774
PpuMI	RGGWCCY	1 cut(s)	1009
PscI	ACATGT	1 cut(s)	1791
Psp1406I	AACGTT	1 cut(s)	2187
Psp5II	RGGWCCY	1 cut(s)	1009
Psp6I	CCWGG	1 cut(s)	1067
PspGI	CCWGG	1 cut(s)	1067
PspN4I	GGNNCC	3 cut(s)	84, 1010, 2141
PspPI	GGNCC	3 cut(s)	185, 445, 1009
PspPPI	RGGWCCY	1 cut(s)	1009
PsuI	RGATCY	4 cut(s)	67, 1141, 1969, 2139
PvuI	CGATCG	1 cut(s)	708
PvuII	CAGCTG	1 cut(s)	1676
RruI	TCGCGA	1 cut(s)	1199
RsaI	GTAC	4 cut(s)	326, 881, 1406, 1796
RsaNI	GTAC	4 cut(s)	325, 880, 1405, 1795
RseI	CAYNNNNRTG	1 cut(s)	1101
SacII	CCGCGG	2 cut(s)	444, 550
SaqAI	TTAA	6 cut(s)	35, 345, 1128, 1610, 1965, 2212
Sau96I	GGNCC	3 cut(s)	185, 445, 1009
ScaI	AGTACT	1 cut(s)	326
SchI	GAGTC	1 cut(s)	1087
ScrFI	CCNGG	5 cut(s)	836, 1069, 1313, 1314, 1880
SduI	GDGCHC	2 cut(s)	222, 1646
SfaNI	GCATC	2 cut(s)	510, 1717
Sfr303I	CCGCGG	2 cut(s)	444, 550
SfuI	TTCGAA	1 cut(s)	788
SgrBI	CCGCGG	2 cut(s)	444, 550
SinI	GGWCC	1 cut(s)	1009
SmaI	CCCGGG	1 cut(s)	1314
SmiMI	CAYNNNNRTG	1 cut(s)	1101
SmlI	CTYRAG	1 cut(s)	1564
SmoI	CTYRAG	1 cut(s)	1564
Sse9I	AATT	9 cut(s)	797, 1110, 1147, 1190, 1464, 1586, 1905, 2089, 2168
SseBI	AGGCCT	1 cut(s)	1940
SspMI	CTAG	1 cut(s)	2199
StuI	AGGCCT	1 cut(s)	1940
StyD4I	CCNGG	5 cut(s)	834, 1067, 1311, 1312, 1878
StyI	CCWWGG	1 cut(s)	2043
TaaI	ACNGT	4 cut(s)	181, 217, 973, 1531
TaiI	ACGT	3 cut(s)	969, 1776, 2190
TaqI	TCGA	6 cut(s)	708, 788, 801, 1061, 1076, 1151
TasI	AATT	9 cut(s)	797, 1110, 1147, 1190, 1464, 1586, 1905, 2089, 2168
TatI	WGTACW	3 cut(s)	324, 879, 1404
TauI	GCSGC	7 cut(s)	154, 362, 446, 552, 726, 1208, 1757
TfiI	GAWTC	8 cut(s)	458, 629, 785, 959, 1031, 1252, 1376, 1747
Tru1I	TTAA	6 cut(s)	35, 345, 1128, 1610, 1965, 2212
Tru9I	TTAA	6 cut(s)	35, 345, 1128, 1610, 1965, 2212
TscAI	CASTG	5 cut(s)	976, 1458, 1662, 1726, 1734
TseFI	GTSAC	2 cut(s)	967, 1354
TseI	GCWGC	4 cut(s)	154, 552, 1676, 1736
Tsp45I	GTSAC	2 cut(s)	967, 1354
TspDTI	ATGAA	6 cut(s)	90, 394, 409, 550, 720, 1023
TspGWI	ACGGA	1 cut(s)	1317
TspMI	CCCGGG	1 cut(s)	1312
TspRI	CASTG	5 cut(s)	976, 1458, 1662, 1726, 1734
VpaK11BI	GGWCC	1 cut(s)	1009
XapI	RAATTY	5 cut(s)	797, 1147, 1905, 2089, 2168
XceI	RCATGY	1 cut(s)	1795
XmaI	CCCGGG	1 cut(s)	1312
XmiI	GTMKAC	1 cut(s)	690
XspI	CTAG	1 cut(s)	2199
ZrmI	AGTACT	1 cut(s)	326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.