Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000403

Physical Location & Seq

Genomic Coordinates Forward (+)

3548010 .. 3550094

Gene Structure 2085 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1048700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGAATGAATATGCAAAAACAAACATGAAAATCAACATGCTTTCTTCTCTTGCTCTCGTCGTCTGCGCACTCTGTTTTTCGGCGTTGATTCCGCTGGCTACCCCCGCCCCATTTCTCTTCCCGGAAGCTCAGTCCACCATCCTCCCCGACCCTTCCACCTTCTTCGCCCCGAACCTCCTCTCATCCCCGCTCCCCACCAACTCCTTCTTCCAAAACTTTGTCCTCAATAATGGCGACCAACCTGAATACATCCACCCCTACACCATCAGATCAGCCAACTCTTCCCTATCTCTCGCTTACCCGTCTTTCTCCTACAACTCTACTGCCGTATCCACAACCCCGTTCGAAGCTGACCTCACCATCTTCGCGTCCAATTCAAACAAGAACGGCCACCATGTAGTCTCCGACTTCAACGATTTGAGTGTGACATTGGACTTTCCCTCCTCCAACCTCCGCTTCTACCTGGTTCGGGGAACCCCCTTTCTCACCTGCTTTGTTTATCGCCCCACGTCGGTTTCTTTATCGACCAATCATAAGATTGTCTCGGCATCCTCAGCGAGTGGCTCAAACACCAAGTTCATCGTCAAGCTCGACAATTATCAGACATGGCTAGTATACACATCCTCCCCAACTGAATTAATTGACAGCGACTCATCCACGTTATCTTTAAAAAAAAATTCTGGGATTATTCGTGTTGCTGTAGTGCCGGATCCTGATCCTAAATCTGAGAAAGTTCTCGACCACTTCAGTTCTTGTTACCCGATTTCTGGTGCAGCTGTGCTCGAAAACTCAAATACTTTGTCGTACAAATTTGAAAAGAAAGGTGGAGATTTGCTTGTGCTAGCTCATCCTCTCCATCTCAAGCTTCTGTCCAATGCTACTGTTTTGCAAAATTTTAAGTACAAAAGCATCGACGGTGATTTGGTTGGCATTGTTGGTGATTTGTGGCTGTTAGCACCGAAGCCTATTCCAGTCACTTGGAATTCCGTTGGAGGTGTAAAAAAGGATGCGATTTCTGAGATCAAATCTGCTATTCGTCGAGATGTTGACGCCCTTAGTTCAACAGAATCAAGTACCAAATCTTCATACTTTTACGGGAAATTAGTTGCTAGAGCGGCGAGGCTGGCTCTGATTGCCGAGGAGGTAGCTTGTTCTGATGTGATCCCTGCGATTAGGACATACTTGAGCGATAAAATTGAGCCGTGGTTGGACGGTGCTTTTAGCGGCAACGGATTTTTGTATGACAAAACATGGGGTGGAATTATTACCAAATCCTCCAGAACTGATCCAACTGCTGATTTCGGGTTTGGTATTTATAACGATCACCATTTTCATCTGGGGTACTTTATATATGGGATTTCCGTGTTAGCAAAGATTGATCCTATATGGGGAGAGAAGTACAAGCCTCAAGCATACTCGCTCGTGGAGGATTTTCTGAACAAGGGCAAGCAATCGAATCCAAATTACGCTCGTCTGAGATGCTTTGATCTTTATAAAATGCACTCGTGGGCGGGAGGGTTGACAGAATTCCCAGACGGGAGGAATCAAGAGAGCACGAGTGAGGCAGTTAATGCCTACTATGCAGCTGCTCTAATGGGGAAAGTTTATAAAGATGCGCAACTTGAGGCCACCGGTTCGCTGCTTACTGCATTAGAAATTCAGGCAGCTCAAATGTGGTGGCATGTGAGAGAGGGAGGAAAGAATCAAATTTACAAGAAAGGTTTCGCGAAGAAAAATCGGGTTGTGGGAATTTTATGGGCCATCAAGAGAGACAGCAAGCTTTGGTTTGCGACTGCAAAGGCAAAAGAAGCCAGACTTGGAATCCAAATGCTGCCTATTTTGCCAGTTACTGAGGTACTGTTCTCTGATGAGGGGTTTGCTAGTGAGCTAGTGAACTGGGCATTACCGGCGTTCAGGAGGAAGGGAGTCACGGACAGATGGAAAGGATTTGGGTATGCATTACAAGGGATGTACGACAAAGAACGCGCTTTGGAGAAGATTAGAAGCTTGAAAGATTTCGACGATGGAAACTCCCTTAGTAATCTGTTATGGTGGATTCACAGTAGATCAACTTCTGTGGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0000746 GO:0000747 GO:0000920 GO:0000935 GO:0000936 GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005937 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006076 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0009986 GO:0009987 GO:0010383 GO:0015629 GO:0015926 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016998 GO:0019953 GO:0022411 GO:0022414 GO:0030246 GO:0030247 GO:0030427 GO:0030428 GO:0030994 GO:0031505 GO:0032153 GO:0032155 GO:0042973 GO:0042995 GO:0043170 GO:0043187 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043332 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044277 GO:0044347 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044457 GO:0044463 GO:0044464 GO:0044703 GO:0044764 GO:0045229 GO:0051273 GO:0051275 GO:0051286 GO:0051301 GO:0051704 GO:0070871 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0071853 GO:0120025 GO:0120038 GO:1901575 GO:1904541 GO:1990819

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_81

Protein Analysis

695

Amino Acids

76.89

Weight (kDa)

8.64

Isoelectric Point (pI)

34.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_81	PF03639	61 - 326	1.5e-53	Glycosyl hydrolase family 81 N-terminal domain
Glyco_hydro81C	PF17652	333 - 685	7.2e-104	Glycosyl hydrolase family 81 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18310 AT5G15870
fragaria_vesca	FvH4_3g44967 FvH4_6g45580 FvH4_6g47860 FvH4_6g47870 FvH4_6g53160
malus_domestica	MD09G1047100.v1.1 MD09G1081400.v1.1 MD09G1081500.v1.1 MD09G1081600.v1.1 MD09G1081700.v1.1 MD15G1128600.v1.1 MD17G1047200.v1.1 MD17G1048700.v1.1 MD17G1071200.v1.1 MD17G1072700.v1.1
prunus_persica	Prupe.3G242000_v2.0.a1 Prupe.3G242100_v2.0.a1 Prupe.3G261700_v2.0.a1 Prupe.8G191700_v2.0.a1 Prupe.8G194600_v2.0.a1 Prupe.8G259500_v2.0.a1
pyrus_communis	pycom09g00810 pycom09g00820 pycom09g00830 pycom15g11570 pycom17g04350 pycom17g07070 pycom17g07350
rosa_chinensis	RchiOBHm_Chr1g0366281 RchiOBHm_Chr2g0163531 RchiOBHm_Chr2g0163581 RchiOBHm_Chr2g0167061 RchiOBHm_Chr2g0167071 RchiOBHm_Chr3g0490821 RchiOBHm_Chr3g0490841 RchiOBHm_Chr3g0492161 RchiOBHm_Chr3g0492171
rosa_laevigata	RLG00000021408 RLG00000021693 RLG00000021694 RLG00000022819 RLG00000022827
rosa_multiflora	Rmu_co8304887.1_g000001 Rmu_co8334235.1_g000001 Rmu_co8488335.1_g000001 Rmu_sc0003484.1_g000001 Rmu_sc0003484.1_g000004 Rmu_sc0010966.1_g000013 Rmu_sc0012462.1_g000001 Rmu_sc0019504.1_g000002 Rmu_sc0019504.1_g000004 Rmu_sc0022540.1_g000001 Rmu_sc0023760.1_g000001 Rmu_sc0038280.1_g000002
rosa_roxburghii	Rroxscaffold_2G00077500 Rroxscaffold_2G00083750 Rroxscaffold_2G00083760 Rroxscaffold_2G00087160 Rroxscaffold_2G00132530 Rroxscaffold_4G00290030 Rroxscaffold_4G00290040 Rroxscaffold_4G00290280
rosa_rugosa	Rorug01G0328400 Rorug02G0265200 Rorug02G0265900 Rorug02G0266000 Rorug02G0266800.1 Rorug02G0266900.1 Rorug02G0267500 Rorug02G0267600 Rorug02G0268300 Rorug02G0268800 Rorug02G0268900 Rorug02G0503600 Rorug02G0528200 Rorug02G0528300 Rorug03G0247200 Rorug03G0247300
rosa_samantha	Rh1BG298400 Rh1DG329800 Rh2AG570500 Rh2AG570700 Rh2AG595000 Rh2AG595100 Rh2AG595200 Rh2CG552500 Rh2CG552700 Rh2CG577100 Rh2CG577200 Rh2DG592500 Rh2DG617800 Rh2DG617900 Rh3CG333000 Rh3DG335000
rosa_wichuraiana	Rw2G047230 Rw2G049530 Rw2G049540 Rw3G026510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1317, 1494, 1608
AarI	CACCTGC	1 cut(s)	497
Acc16I	TGCGCA	2 cut(s)	67, 1617
Acc36I	ACCTGC	1 cut(s)	497
AccBSI	CCGCTC	2 cut(s)	190, 1115
AccI	GTMKAC	1 cut(s)	615
AccII	CGCG	3 cut(s)	368, 1727, 1986
AciI	CCGC	7 cut(s)	92, 105, 188, 454, 1115, 1224, 1511
AclWI	GGATC	6 cut(s)	704, 710, 717, 1156, 1280, 1373
AcoI	YGGCCR	1 cut(s)	388
AcsI	RAATTY	8 cut(s)	676, 809, 892, 982, 1526, 1656, 1707, 1749
AcuI	CTGAAG	1 cut(s)	730
AcyI	GRCGYC	1 cut(s)	1050
AfaI	GTAC	7 cut(s)	806, 902, 1075, 1343, 1400, 1857, 1973
AfiI	CCNNNNNNNGG	4 cut(s)	469, 511, 767, 1388
AgeI	ACCGGT	1 cut(s)	1631
AgsI	TTSAA	5 cut(s)	378, 412, 815, 1062, 2011
AjiI	CACGTC	1 cut(s)	510
AjnI	CCWGG	1 cut(s)	462
AjuI	GAANNNNNNNTTGG	6 cut(s)	191, 223, 440, 472, 1800, 1832
Alw21I	GWGCWC	2 cut(s)	783, 1556
Alw26I	GTCTC	3 cut(s)	406, 547, 1764
AlwI	GGATC	6 cut(s)	704, 710, 717, 1156, 1280, 1373
AlwNI	CAGNNNCTG	1 cut(s)	1850
AoxI	GGCC	3 cut(s)	388, 1626, 1758
ApeKI	GCWGC	6 cut(s)	773, 1583, 1586, 1639, 1664, 1831
ApoI	RAATTY	8 cut(s)	676, 809, 892, 982, 1526, 1656, 1707, 1749
ArsI	GACNNNNNNTTYG	2 cut(s)	785, 817
AseI	ATTAAT	1 cut(s)	638
AsiGI	ACCGGT	1 cut(s)	1631
Asp700I	GAANNNNTTC	2 cut(s)	1721, 2015
AspLEI	GCGC	3 cut(s)	68, 1618, 1988
AspS9I	GGNCC	1 cut(s)	1758
AsuC2I	CCSGG	1 cut(s)	122
AsuHPI	GGTGA	5 cut(s)	349, 478, 929, 950, 1316
AsuII	TTCGAA	1 cut(s)	345
AsuNHI	GCTAGC	1 cut(s)	841
BaeI	ACNNNNGTAYC	2 cut(s)	312, 345
BamHI	GGATCC	1 cut(s)	709
BarI	GAAGNNNNNNTAC	3 cut(s)	1066, 1098, 2056
BauI	CACGAG	3 cut(s)	1421, 1504, 1555
Bbv12I	GWGCWC	2 cut(s)	783, 1556
BbvCI	CCTCAGC	1 cut(s)	553
BbvI	GCAGC	6 cut(s)	785, 1573, 1595, 1626, 1676, 1818
BccI	CCATC	7 cut(s)	146, 272, 368, 864, 1769, 1932, 2018
BceAI	ACGGC	3 cut(s)	311, 403, 1186
BciT130I	CCWGG	1 cut(s)	464
BciVI	GTATCC	1 cut(s)	340
BcnI	CCSGG	1 cut(s)	122
BcoDI	GTCTC	3 cut(s)	406, 547, 1764
BfaI	CTAG	5 cut(s)	611, 842, 1110, 1881, 1889
BfmI	CTRYAG	1 cut(s)	699
BfuAI	ACCTGC	1 cut(s)	497
BfuI	GTATCC	1 cut(s)	340
BisI	GCNGC	8 cut(s)	774, 1116, 1225, 1584, 1587, 1640, 1665, 1832
BlsI	GCNGC	8 cut(s)	775, 1117, 1226, 1585, 1588, 1641, 1666, 1833
Bme1390I	CCNGG	2 cut(s)	122, 464
BmgBI	CACGTC	1 cut(s)	510
BmgT120I	GGNCC	1 cut(s)	1758
BmiI	GGNNCC	2 cut(s)	475, 711
BmrFI	CCNGG	2 cut(s)	122, 464
BmrI	ACTGGG	1 cut(s)	1906
BmsI	GCATC	5 cut(s)	557, 918, 997, 1469, 1603
BmtI	GCTAGC	1 cut(s)	845
BmuI	ACTGGG	1 cut(s)	1906
BplI	GAGNNNNNCTC	2 cut(s)	1111, 1143
BpmI	CTGGAG	1 cut(s)	1261
Bpu10I	CCTNAGC	1 cut(s)	553
Bpu14I	TTCGAA	1 cut(s)	345
BpuEI	CTTGAG	4 cut(s)	845, 1204, 1392, 1643
BpuMI	CCSGG	1 cut(s)	122
BsaBI	GATNNNNATC	1 cut(s)	714
BsaHI	GRCGYC	1 cut(s)	1050
BsaJI	CCNNGG	2 cut(s)	1137, 1202
BsaWI	WCCGGW	1 cut(s)	1631
BsaXI	ACNNNNNCTCC	4 cut(s)	425, 455, 608, 638
Bsc4I	CCNNNNNNNGG	4 cut(s)	469, 511, 767, 1388
Bse118I	RCCGGY	2 cut(s)	1631, 1906
Bse1I	ACTGG	3 cut(s)	971, 1844, 1901
Bse8I	GATNNNNATC	1 cut(s)	714
BseBI	CCWGG	1 cut(s)	464
BseDI	CCNNGG	2 cut(s)	1137, 1202
BseGI	GGATG	9 cut(s)	138, 182, 249, 548, 620, 653, 847, 1012, 1974
BseJI	GATNNNNATC	1 cut(s)	714
BseLI	CCNNNNNNNGG	4 cut(s)	469, 511, 767, 1388
BseMII	CTCAG	6 cut(s)	143, 567, 717, 1008, 1466, 1842
BseNI	ACTGG	3 cut(s)	971, 1844, 1901
BseRI	GAGGAG	3 cut(s)	167, 433, 1154
BseXI	GCAGC	6 cut(s)	785, 1573, 1595, 1626, 1676, 1818
BsgI	GTGCAG	1 cut(s)	792
Bsh1236I	CGCG	3 cut(s)	368, 1727, 1986
BshFI	GGCC	3 cut(s)	390, 1628, 1760
BshTI	ACCGGT	1 cut(s)	1631
BsiHKAI	GWGCWC	2 cut(s)	783, 1556
BsiSI	CCGG	4 cut(s)	122, 707, 1632, 1907
BslI	CCNNNNNNNGG	4 cut(s)	469, 511, 767, 1388
BsmAI	GTCTC	3 cut(s)	406, 547, 1764
BsnI	GGCC	3 cut(s)	390, 1628, 1760
Bsp119I	TTCGAA	1 cut(s)	345
Bsp1286I	GDGCHC	2 cut(s)	783, 1556
Bsp68I	TCGCGA	1 cut(s)	1727
BspACI	CCGC	7 cut(s)	92, 105, 188, 454, 1115, 1224, 1511
BspANI	GGCC	3 cut(s)	390, 1628, 1760
BspCNI	CTCAG	6 cut(s)	142, 566, 718, 1009, 1467, 1843
BspFNI	CGCG	3 cut(s)	368, 1727, 1986
BspLI	GGNNCC	2 cut(s)	475, 711
BspMI	ACCTGC	1 cut(s)	497
BspOI	GCTAGC	1 cut(s)	845
BspPI	GGATC	6 cut(s)	704, 710, 717, 1156, 1280, 1373
BspT104I	TTCGAA	1 cut(s)	345
BsrBI	CCGCTC	2 cut(s)	190, 1115
BsrFI	RCCGGY	2 cut(s)	1631, 1906
BsrI	ACTGG	3 cut(s)	971, 1844, 1901
BssAI	RCCGGY	2 cut(s)	1631, 1906
BssECI	CCNNGG	2 cut(s)	1137, 1202
BssNAI	GTATAC	1 cut(s)	616
BssNI	GRCGYC	1 cut(s)	1050
BssSI	CACGAG	3 cut(s)	1421, 1504, 1555
Bst1107I	GTATAC	1 cut(s)	616
Bst2BI	CACGAG	3 cut(s)	1421, 1504, 1555
Bst2UI	CCWGG	1 cut(s)	464
Bst4CI	ACNGT	5 cut(s)	883, 917, 1214, 1860, 2063
Bst6I	CTCTTC	2 cut(s)	122, 286
BstACI	GRCGYC	1 cut(s)	1050
BstAPI	GCANNNNNTGC	1 cut(s)	1571
BstBI	TTCGAA	1 cut(s)	345
BstC8I	GCNNGC	5 cut(s)	96, 843, 1125, 1448, 1778
BstDEI	CTNAG	8 cut(s)	129, 553, 726, 1017, 1055, 1475, 1851, 2036
BstDSI	CCRYGG	1 cut(s)	1202
BstF5I	GGATG	9 cut(s)	138, 182, 249, 548, 620, 653, 847, 1012, 1974
BstFNI	CGCG	3 cut(s)	368, 1727, 1986
BstHHI	GCGC	3 cut(s)	68, 1618, 1988
BstMAI	GTCTC	3 cut(s)	406, 547, 1764
BstNI	CCWGG	1 cut(s)	464
BstNSI	RCATGY	2 cut(s)	40, 1685
BstSCI	CCNGG	2 cut(s)	120, 462
BstSFI	CTRYAG	1 cut(s)	699
BstUI	CGCG	3 cut(s)	368, 1727, 1986
BstV1I	GCAGC	6 cut(s)	785, 1573, 1595, 1626, 1676, 1818
BstX2I	RGATCY	1 cut(s)	709
BstXI	CCANNNNNNTGG	1 cut(s)	978
BstYI	RGATCY	1 cut(s)	709
BstZ17I	GTATAC	1 cut(s)	616
BsuI	GTATCC	1 cut(s)	340
BsuRI	GGCC	3 cut(s)	390, 1628, 1760
BtgI	CCRYGG	1 cut(s)	1202
BtrI	CACGTC	1 cut(s)	510
BtsCI	GGATG	9 cut(s)	138, 182, 249, 548, 620, 653, 847, 1012, 1974
BtuMI	TCGCGA	1 cut(s)	1727
BveI	ACCTGC	1 cut(s)	497
Cac8I	GCNNGC	5 cut(s)	96, 843, 1125, 1448, 1778
CaiI	CAGNNNCTG	1 cut(s)	1850
CfoI	GCGC	3 cut(s)	68, 1618, 1988
Cfr10I	RCCGGY	2 cut(s)	1631, 1906
Cfr13I	GGNCC	1 cut(s)	1758
CseI	GACGC	2 cut(s)	357, 1058
CsiI	ACCWGGT	1 cut(s)	462
Csp6I	GTAC	7 cut(s)	805, 901, 1074, 1342, 1399, 1856, 1972
CspAI	ACCGGT	1 cut(s)	1631
CspCI	CAANNNNNGTGG	3 cut(s)	1658, 1693, 2058
CviAII	CATG	6 cut(s)	25, 37, 395, 606, 1251, 1682
CviQI	GTAC	7 cut(s)	805, 901, 1074, 1342, 1399, 1856, 1972
DdeI	CTNAG	8 cut(s)	129, 553, 726, 1017, 1055, 1475, 1851, 2036
DraI	TTTAAA	1 cut(s)	669
EaeI	YGGCCR	1 cut(s)	388
Eam1104I	CTCTTC	2 cut(s)	122, 286
EarI	CTCTTC	2 cut(s)	122, 286
Eco57I	CTGAAG	1 cut(s)	730
EcoRI	GAATTC	2 cut(s)	982, 1526
EcoRII	CCWGG	1 cut(s)	462
EcoT22I	ATGCAT	1 cut(s)	1960
FaeI	CATG	6 cut(s)	28, 40, 398, 609, 1254, 1685
FalI	AAGNNNNNCTT	2 cut(s)	1800, 1832
FatI	CATG	6 cut(s)	24, 36, 394, 605, 1250, 1681
FauI	CCCGC	3 cut(s)	112, 195, 1504
FblI	GTMKAC	1 cut(s)	615
Fnu4HI	GCNGC	8 cut(s)	774, 1116, 1225, 1584, 1587, 1640, 1665, 1832
FokI	GGATG	9 cut(s)	125, 169, 236, 535, 607, 640, 834, 1019, 1981
Fsp4HI	GCNGC	8 cut(s)	774, 1116, 1225, 1584, 1587, 1640, 1665, 1832
FspBI	CTAG	5 cut(s)	611, 842, 1110, 1881, 1889
FspI	TGCGCA	2 cut(s)	67, 1617
GlaI	GCGC	3 cut(s)	67, 1617, 1987
GluI	GCNGC	8 cut(s)	774, 1116, 1225, 1584, 1587, 1640, 1665, 1832
GsuI	CTGGAG	1 cut(s)	1261
HaeIII	GGCC	3 cut(s)	390, 1628, 1760
HapII	CCGG	4 cut(s)	122, 707, 1632, 1907
HgaI	GACGC	2 cut(s)	357, 1058
HhaI	GCGC	3 cut(s)	68, 1618, 1988
Hin1I	GRCGYC	1 cut(s)	1050
Hin1II	CATG	6 cut(s)	28, 40, 398, 609, 1254, 1685
Hin6I	GCGC	3 cut(s)	66, 1616, 1986
HinP1I	GCGC	3 cut(s)	66, 1616, 1986
HincII	GTYRAC	2 cut(s)	1048, 1521
HindII	GTYRAC	2 cut(s)	1048, 1521
HindIII	AAGCTT	3 cut(s)	863, 1778, 2005
HinfI	GANTC	9 cut(s)	88, 650, 1067, 1456, 1543, 1702, 1821, 1926, 2056
HpaII	CCGG	4 cut(s)	122, 707, 1632, 1907
HphI	GGTGA	5 cut(s)	349, 478, 929, 950, 1316
Hpy166II	GTNNAC	5 cut(s)	135, 616, 1048, 1521, 1894
Hpy188III	TCNNGA	8 cut(s)	713, 737, 1040, 1278, 1547, 1726, 1765, 1915
Hpy8I	GTNNAC	5 cut(s)	135, 616, 1048, 1521, 1894
Hpy99I	CGWCG	5 cut(s)	62, 514, 917, 1041, 2024
HpyAV	CCTTC	4 cut(s)	162, 169, 214, 1915
HpyCH4III	ACNGT	5 cut(s)	883, 917, 1214, 1860, 2063
HpyCH4IV	ACGT	2 cut(s)	509, 659
HpyCH4V	TGCA	8 cut(s)	14, 773, 889, 1501, 1583, 1649, 1796, 1958
HpyF3I	CTNAG	8 cut(s)	129, 553, 726, 1017, 1055, 1475, 1851, 2036
HpySE526I	ACGT	2 cut(s)	509, 659
Hsp92I	GRCGYC	1 cut(s)	1050
Hsp92II	CATG	6 cut(s)	28, 40, 398, 609, 1254, 1685
HspAI	GCGC	3 cut(s)	66, 1616, 1986
LmnI	GCTCC	1 cut(s)	195
Lsp1109I	GCAGC	6 cut(s)	785, 1573, 1595, 1626, 1676, 1818
LweI	GCATC	5 cut(s)	557, 918, 997, 1469, 1603
MabI	ACCWGGT	1 cut(s)	462
MaeI	CTAG	5 cut(s)	611, 842, 1110, 1881, 1889
MaeII	ACGT	2 cut(s)	509, 659
MaeIII	GTNAC	5 cut(s)	424, 755, 973, 1846, 1927
MbiI	CCGCTC	2 cut(s)	190, 1115
MboII	GAAGA	9 cut(s)	36, 109, 154, 199, 273, 355, 1074, 1741, 2008
MflI	RGATCY	1 cut(s)	709
MhlI	GDGCHC	2 cut(s)	783, 1556
MlyI	GAGTC	2 cut(s)	644, 1935
MmeI	TCCRAC	5 cut(s)	429, 471, 970, 1188, 1313
Mph1103I	ATGCAT	1 cut(s)	1960
MroXI	GAANNNNTTC	2 cut(s)	1721, 2015
MseI	TTAA	5 cut(s)	638, 668, 897, 1569, 2083
MspA1I	CMGCKG	3 cut(s)	94, 776, 1586
MspI	CCGG	4 cut(s)	122, 707, 1632, 1907
MspR9I	CCNGG	2 cut(s)	122, 464
MvaI	CCWGG	1 cut(s)	464
MvnI	CGCG	3 cut(s)	368, 1727, 1986
NciI	CCSGG	1 cut(s)	122
NheI	GCTAGC	1 cut(s)	841
NlaIII	CATG	6 cut(s)	28, 40, 398, 609, 1254, 1685
NlaIV	GGNNCC	2 cut(s)	475, 711
NmeAIII	GCCGAG	2 cut(s)	524, 1162
NmuCI	GTSAC	3 cut(s)	424, 973, 1927
NruI	TCGCGA	1 cut(s)	1727
NsbI	TGCGCA	2 cut(s)	67, 1617
NsiI	ATGCAT	1 cut(s)	1960
NspI	RCATGY	2 cut(s)	40, 1685
NspV	TTCGAA	1 cut(s)	345
PaqCI	CACCTGC	1 cut(s)	497
PcsI	WCGNNNNNNNCGW	1 cut(s)	588
PdmI	GAANNNNTTC	2 cut(s)	1721, 2015
PfeI	GAWTC	7 cut(s)	88, 1067, 1456, 1543, 1702, 1821, 2056
PfoI	TCCNGGA	1 cut(s)	120
PinAI	ACCGGT	1 cut(s)	1631
PkrI	GCNGC	8 cut(s)	775, 1117, 1226, 1585, 1588, 1641, 1666, 1833
PleI	GAGTC	2 cut(s)	644, 1934
PpsI	GAGTC	2 cut(s)	644, 1934
PshBI	ATTAAT	1 cut(s)	638
PsiI	TTATAA	3 cut(s)	1317, 1494, 1608
Psp6I	CCWGG	1 cut(s)	462
PspGI	CCWGG	1 cut(s)	462
PspN4I	GGNNCC	2 cut(s)	475, 711
PspPI	GGNCC	1 cut(s)	1758
PstNI	CAGNNNCTG	1 cut(s)	1850
PsuI	RGATCY	1 cut(s)	709
PvuII	CAGCTG	2 cut(s)	776, 1586
RruI	TCGCGA	1 cut(s)	1727
RsaI	GTAC	7 cut(s)	806, 902, 1075, 1343, 1400, 1857, 1973
RsaNI	GTAC	7 cut(s)	805, 901, 1074, 1342, 1399, 1856, 1972
SaqAI	TTAA	5 cut(s)	638, 668, 897, 1569, 2083
SatI	GCNGC	8 cut(s)	774, 1116, 1225, 1584, 1587, 1640, 1665, 1832
Sau96I	GGNCC	1 cut(s)	1758
SchI	GAGTC	2 cut(s)	644, 1935
ScrFI	CCNGG	2 cut(s)	122, 464
SduI	GDGCHC	2 cut(s)	783, 1556
SexAI	ACCWGGT	1 cut(s)	462
SfaNI	GCATC	5 cut(s)	557, 918, 997, 1469, 1603
SfcI	CTRYAG	1 cut(s)	699
SfuI	TTCGAA	1 cut(s)	345
SmlI	CTYRAG	4 cut(s)	860, 1183, 1407, 1622
SmoI	CTYRAG	4 cut(s)	860, 1183, 1407, 1622
SsiI	CCGC	7 cut(s)	92, 105, 188, 454, 1115, 1224, 1511
SspMI	CTAG	5 cut(s)	611, 842, 1110, 1881, 1889
StyD4I	CCNGG	2 cut(s)	120, 462
TaaI	ACNGT	5 cut(s)	883, 917, 1214, 1860, 2063
TaiI	ACGT	2 cut(s)	512, 662
TaqI	TCGA	9 cut(s)	345, 524, 591, 738, 783, 912, 1039, 1454, 2019
TatI	WGTACW	2 cut(s)	900, 1398
TauI	GCSGC	2 cut(s)	1118, 1227
TfiI	GAWTC	7 cut(s)	88, 1067, 1456, 1543, 1702, 1821, 2056
Tru1I	TTAA	5 cut(s)	638, 668, 897, 1569, 2083
Tru9I	TTAA	5 cut(s)	638, 668, 897, 1569, 2083
TseFI	GTSAC	3 cut(s)	424, 973, 1927
TseI	GCWGC	6 cut(s)	773, 1583, 1586, 1639, 1664, 1831
Tsp45I	GTSAC	3 cut(s)	424, 973, 1927
TspDTI	ATGAA	6 cut(s)	17, 21, 41, 568, 1074, 1322
TspGWI	ACGGA	4 cut(s)	976, 1245, 1351, 1946
VspI	ATTAAT	1 cut(s)	638
XapI	RAATTY	8 cut(s)	676, 809, 892, 982, 1526, 1656, 1707, 1749
XceI	RCATGY	2 cut(s)	40, 1685
XmiI	GTMKAC	1 cut(s)	615
XmnI	GAANNNNTTC	2 cut(s)	1721, 2015
XspI	CTAG	5 cut(s)	611, 842, 1110, 1881, 1889
Zsp2I	ATGCAT	1 cut(s)	1960

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.