Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000403

Physical Location & Seq

Genomic Coordinates Reverse (-)

3449024 .. 3451054

Gene Structure 2031 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1047200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGCTCTCTCCTCTTGCTCTCGTCGTCTGCGTACTGTGTTTTTCGGCATTGATTCCGCAGGCTACCCCCGCCCCGTTCCTCTTCCCGGAAGCTCAGTCCACCATCCTCCCCGACCCTTCAACCTTCTTCGCCTCGAACCTCCTCTCATCCCCGCTCCCCACCAACTCCTTCTTCCAAAACTTTGTCCTCGGCAATGGCGATCAACCTGAATACATCCACCCCTACACCATCAGATCAGCCAACTCTTCTCTATCTCTTGCTTACCCGTCTTTCTCCTACTCCTCTACCGTCGTATCCACAACCCCGTTCGAAGCCGACCTCACCATCGCCTCAACTTCAAATAGCAACGGTCACCATGTAGTGTCCGACTTCAATGATTTGAGTGTGACATTGGACTTTCCCTCCTCCAACCTCCGCTTCTACCTGGTTCGAGGAAGCCCCTTTCTCACCTGCTTTGTTTATCGTCCCATGTCGGTTTCTATATCGACCGATCATACGATTGTCTCGGCATCCTCAGCGAGTGGCTCGAGCACCAAGTTCATCGTCAAGCTCGACAATGATCACACATGGCTAATATATACATCCTCACCAACTGAATTTACTGACAGCGACTCATCCACGTTAACTCTTAAAGAATTTTCTGGGATTTTTCGTGTTGCTGTAATGCCGGATCCTGATCCTAAATCTGAGAAAGTTCTCGACCACTTCAGTTCTTGTTACCCGATTTCTGGTGCAGCTGTGCTCGAAAACTCAAATACTTTGTCGTATAAGTTCGGAAAGATAGGTGGAGACTTGCTCATGCTAGCTCATCCTCTACATCTCAAGCTTCTGTCCAACGCCACAATTTTGCAAGATTTTAAGTACAAAAGCATCGACGGGGATTTGATTGGCGTCGTTGGTGATTCGTGGGTGTTAGCGTCGAAGCCTATTCCAGTCACTTGGAATTCCGTTGGAGGTGTCAAAAAGGATTCGTTTTCAGAGATCATATCTGCACTTCGTCGAGATGTTGACGCTCTTAGTTCATCAGAATCAACTACCACGTCTTCGTACTTTTACGGGAAACTAGTTGCTAGAGCTGCGAGGCTGGCTCTGATCGCCGAGGAGGTAGCCTGTCCTGATGTGATCCCTGCGATTAGGACATTCTTGAGCAATAAAATCGAGCCGTGGTTGGATGGTACTTTTAGTGGCAACGGATTCTTGCATGATAAAACATGGGGTGGACTTGTTACCAAATCCTCCGCAACTGATCCAGCTGCAGATTTCGGGTTTGGTCTTTATTCAGATCACCATTTTCATCTGGGGTACTTTATATATGCAATTTCCGTGTTAGCAAAGATTGATCCTGCATGGGGAGAGAAGTACAAGCCTCAAGCTTACTTGCTCGTGGAGGATTTTCTCAACAAGGACAAGAAATTGAATCCAAATTACACGCGTCTGAGATGCTTTGATCTGTATAAGTTGCACTCGTGGGCGGCAGGGTTGACAGAATTCGCAGACGGGAGGAATCAAGAGAGTACGAGTGAGGCAGTTAATGCGTACTATGCAGCCGCTCTGATGGGGAAAGCGTATAAAGATGCGCAACTTGAGGCCACCGGTTCGCTGCTTACTGCATTAGAAATTCAGGCAGCTCAAATGTGGTGGCATGTGAGAGAGGGAGGAGAGAATCAAATTTACGAGCAAGATTTCGCGAAGGAGAATCGGGTTGTGGGAGTTTTATGGTCGACCAAGAGAGAGAGCGGGCTTTGGTTTGCGACTGCGGAGGCAAAAGAAATCAGACTTGGAATCCAAATGGTGCCTGTTTTGCCAGTTACTGAGGTATTGTTCGCTGATGTCGGGTTTGCTAGTGAGCTAGTGAACTGGGCATCGCCGGCGCTCAGCAGGACGGGAGTCACGGAAGCATGGAAAGGATTTGTGTATGCATTACAAGGGATGTACGACAAAGAAGGCGCTTTGGAGAAGATTAGAAGCTTGGAAGAATTCGACGATGGAAACTCCCGCAGTAATCTCTTATGGTGGATTCACAGTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0000746 GO:0000747 GO:0000920 GO:0000935 GO:0000936 GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005937 GO:0005975 GO:0005976 GO:0006073 GO:0006074 GO:0006076 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0009986 GO:0009987 GO:0010383 GO:0015629 GO:0015926 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016998 GO:0019953 GO:0022411 GO:0022414 GO:0030246 GO:0030247 GO:0030427 GO:0030428 GO:0030994 GO:0031505 GO:0032153 GO:0032155 GO:0042973 GO:0042995 GO:0043170 GO:0043187 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043332 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044277 GO:0044347 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044457 GO:0044463 GO:0044464 GO:0044703 GO:0044764 GO:0045229 GO:0051273 GO:0051275 GO:0051286 GO:0051301 GO:0051704 GO:0070871 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:0071853 GO:0120025 GO:0120038 GO:1901575 GO:1904541 GO:1990819

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_81

Protein Analysis

677

Amino Acids

74.43

Weight (kDa)

5.28

Isoelectric Point (pI)

38.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_81	PF03639	48 - 313	6.6e-55	Glycosyl hydrolase family 81 N-terminal domain
Glyco_hydro81C	PF17652	320 - 672	4.7e-103	Glycosyl hydrolase family 81 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000403)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18310 AT5G15870
fragaria_vesca	FvH4_3g44967 FvH4_6g45580 FvH4_6g47860 FvH4_6g47870 FvH4_6g53160
malus_domestica	MD09G1047100.v1.1 MD09G1081400.v1.1 MD09G1081500.v1.1 MD09G1081600.v1.1 MD09G1081700.v1.1 MD15G1128600.v1.1 MD17G1047200.v1.1 MD17G1048700.v1.1 MD17G1071200.v1.1 MD17G1072700.v1.1
prunus_persica	Prupe.3G242000_v2.0.a1 Prupe.3G242100_v2.0.a1 Prupe.3G261700_v2.0.a1 Prupe.8G191700_v2.0.a1 Prupe.8G194600_v2.0.a1 Prupe.8G259500_v2.0.a1
pyrus_communis	pycom09g00810 pycom09g00820 pycom09g00830 pycom15g11570 pycom17g04350 pycom17g07070 pycom17g07350
rosa_chinensis	RchiOBHm_Chr1g0366281 RchiOBHm_Chr2g0163531 RchiOBHm_Chr2g0163581 RchiOBHm_Chr2g0167061 RchiOBHm_Chr2g0167071 RchiOBHm_Chr3g0490821 RchiOBHm_Chr3g0490841 RchiOBHm_Chr3g0492161 RchiOBHm_Chr3g0492171
rosa_laevigata	RLG00000021408 RLG00000021693 RLG00000021694 RLG00000022819 RLG00000022827
rosa_multiflora	Rmu_co8304887.1_g000001 Rmu_co8334235.1_g000001 Rmu_co8488335.1_g000001 Rmu_sc0003484.1_g000001 Rmu_sc0003484.1_g000004 Rmu_sc0010966.1_g000013 Rmu_sc0012462.1_g000001 Rmu_sc0019504.1_g000002 Rmu_sc0019504.1_g000004 Rmu_sc0022540.1_g000001 Rmu_sc0023760.1_g000001 Rmu_sc0038280.1_g000002
rosa_roxburghii	Rroxscaffold_2G00077500 Rroxscaffold_2G00083750 Rroxscaffold_2G00083760 Rroxscaffold_2G00087160 Rroxscaffold_2G00132530 Rroxscaffold_4G00290030 Rroxscaffold_4G00290040 Rroxscaffold_4G00290280
rosa_rugosa	Rorug01G0328400 Rorug02G0265200 Rorug02G0265900 Rorug02G0266000 Rorug02G0266800.1 Rorug02G0266900.1 Rorug02G0267500 Rorug02G0267600 Rorug02G0268300 Rorug02G0268800 Rorug02G0268900 Rorug02G0503600 Rorug02G0528200 Rorug02G0528300 Rorug03G0247200 Rorug03G0247300
rosa_samantha	Rh1BG298400 Rh1DG329800 Rh2AG570500 Rh2AG570700 Rh2AG595000 Rh2AG595100 Rh2AG595200 Rh2CG552500 Rh2CG552700 Rh2CG577100 Rh2CG577200 Rh2DG592500 Rh2DG617800 Rh2DG617900 Rh3CG333000 Rh3DG335000
rosa_wichuraiana	Rw2G047230 Rw2G049530 Rw2G049540 Rw3G026510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	458
Acc16I	TGCGCA	1 cut(s)	1578
Acc36I	ACCTGC	1 cut(s)	458
AccB1I	GGYRCC	1 cut(s)	1792
AccBSI	CCGCTC	3 cut(s)	154, 1550, 1737
AccI	GTMKAC	1 cut(s)	1721
AccII	CGCG	2 cut(s)	1432, 1688
AclWI	GGATC	6 cut(s)	665, 671, 678, 1117, 1241, 1334
AcsI	RAATTY	7 cut(s)	596, 635, 943, 1487, 1617, 1668, 1976
AcuI	CTGAAG	1 cut(s)	691
AcyI	GRCGYC	1 cut(s)	891
AfaI	GTAC	9 cut(s)	33, 863, 1049, 1177, 1304, 1361, 1516, 1538, 1934
AfiI	CCNNNNNNNGG	3 cut(s)	85, 728, 1349
AflIII	ACRYGT	1 cut(s)	1430
AgeI	ACCGGT	1 cut(s)	1592
AgsI	TTSAA	4 cut(s)	120, 339, 373, 1417
AhlI	ACTAGT	1 cut(s)	1063
AjiI	CACGTC	1 cut(s)	1041
AjnI	CCWGG	1 cut(s)	423
AjuI	GAANNNNNNNTTGG	6 cut(s)	155, 187, 401, 433, 1761, 1793
Alw21I	GWGCWC	2 cut(s)	533, 744
Alw26I	GTCTC	2 cut(s)	508, 783
AlwI	GGATC	6 cut(s)	665, 671, 678, 1117, 1241, 1334
AlwNI	CAGNNNCTG	1 cut(s)	1811
Ama87I	CYCGRG	1 cut(s)	526
AoxI	GGCC	1 cut(s)	1587
ApeKI	GCWGC	6 cut(s)	734, 1076, 1253, 1544, 1600, 1625
ApoI	RAATTY	7 cut(s)	596, 635, 943, 1487, 1617, 1668, 1976
ArsI	GACNNNNNNTTYG	2 cut(s)	746, 778
AsiGI	ACCGGT	1 cut(s)	1592
AspLEI	GCGC	3 cut(s)	1579, 1873, 1949
AsuC2I	CCSGG	1 cut(s)	86
AsuHPI	GGTGA	6 cut(s)	313, 344, 439, 579, 911, 1277
AsuII	TTCGAA	1 cut(s)	309
AsuNHI	GCTAGC	1 cut(s)	802
AvaI	CYCGRG	1 cut(s)	526
BaeI	ACNNNNGTAYC	2 cut(s)	276, 309
BamHI	GGATCC	1 cut(s)	670
BanI	GGYRCC	1 cut(s)	1792
BauI	CACGAG	2 cut(s)	1382, 1465
BbsI	GAAGAC	1 cut(s)	1035
Bbv12I	GWGCWC	2 cut(s)	533, 744
BbvCI	CCTCAGC	1 cut(s)	514
BbvI	GCAGC	6 cut(s)	746, 1063, 1240, 1556, 1587, 1637
BccI	CCATC	6 cut(s)	110, 236, 332, 1166, 1549, 1979
BceAI	ACGGC	1 cut(s)	1147
BcgI	CGANNNNNNTGC	2 cut(s)	1138, 1172
BciT130I	CCWGG	1 cut(s)	425
BciVI	GTATCC	1 cut(s)	304
BclI	TGATCA	1 cut(s)	559
BcnI	CCSGG	1 cut(s)	86
BcoDI	GTCTC	2 cut(s)	508, 783
BcuI	ACTAGT	1 cut(s)	1063
BfaI	CTAG	5 cut(s)	803, 1064, 1071, 1842, 1850
BfmI	CTRYAG	1 cut(s)	1254
BfoI	RGCGCY	2 cut(s)	1874, 1950
BfuAI	ACCTGC	1 cut(s)	458
BfuI	GTATCC	1 cut(s)	304
BisI	GCNGC	8 cut(s)	735, 1077, 1254, 1473, 1545, 1548, 1601, 1626
BlpI	GCTNAGC	1 cut(s)	1874
BlsI	GCNGC	8 cut(s)	736, 1078, 1255, 1474, 1546, 1549, 1602, 1627
Bme1390I	CCNGG	2 cut(s)	86, 425
BmeT110I	CYCGRG	1 cut(s)	526
BmgBI	CACGTC	1 cut(s)	1041
BmiI	GGNNCC	2 cut(s)	672, 1794
BmrFI	CCNGG	2 cut(s)	86, 425
BmrI	ACTGGG	1 cut(s)	1867
BmsI	GCATC	5 cut(s)	518, 879, 1430, 1564, 1871
BmtI	GCTAGC	1 cut(s)	806
BmuI	ACTGGG	1 cut(s)	1867
BoxI	GACNNNNGTC	1 cut(s)	1886
BpiI	GAAGAC	1 cut(s)	1035
BplI	GAGNNNNNCTC	4 cut(s)	780, 812, 1072, 1104
Bpu10I	CCTNAGC	1 cut(s)	514
Bpu1102I	GCTNAGC	1 cut(s)	1874
Bpu14I	TTCGAA	1 cut(s)	309
BpuEI	CTTGAG	4 cut(s)	806, 1165, 1353, 1604
BpuMI	CCSGG	1 cut(s)	86
BsaBI	GATNNNNATC	1 cut(s)	675
BsaHI	GRCGYC	1 cut(s)	891
BsaJI	CCNNGG	3 cut(s)	187, 1098, 1163
BsaWI	WCCGGW	1 cut(s)	1592
BsaXI	ACNNNNNCTCC	2 cut(s)	386, 416
Bsc4I	CCNNNNNNNGG	3 cut(s)	85, 728, 1349
Bse118I	RCCGGY	2 cut(s)	1592, 1867
Bse1I	ACTGG	3 cut(s)	932, 1805, 1862
Bse3DI	GCAATG	1 cut(s)	199
Bse8I	GATNNNNATC	1 cut(s)	675
BseBI	CCWGG	1 cut(s)	425
BseDI	CCNNGG	3 cut(s)	187, 1098, 1163
BseGI	GGATG	9 cut(s)	102, 146, 213, 509, 581, 614, 808, 1177, 1935
BseJI	GATNNNNATC	1 cut(s)	675
BseLI	CCNNNNNNNGG	3 cut(s)	85, 728, 1349
BseMI	GCAATG	1 cut(s)	199
BseMII	CTCAG	6 cut(s)	107, 528, 678, 1427, 1803, 1888
BseNI	ACTGG	3 cut(s)	932, 1805, 1862
BseRI	GAGGAG	5 cut(s)	131, 271, 394, 1115, 1671
BseXI	GCAGC	6 cut(s)	746, 1063, 1240, 1556, 1587, 1637
BsgI	GTGCAG	2 cut(s)	753, 975
Bsh1236I	CGCG	2 cut(s)	1432, 1688
Bsh1285I	CGRYCG	1 cut(s)	489
BshFI	GGCC	1 cut(s)	1589
BshNI	GGYRCC	1 cut(s)	1792
BshTI	ACCGGT	1 cut(s)	1592
BsiEI	CGRYCG	1 cut(s)	489
BsiHKAI	GWGCWC	2 cut(s)	533, 744
BsiHKCI	CYCGRG	1 cut(s)	526
BsiSI	CCGG	4 cut(s)	86, 668, 1593, 1868
BslFI	GGGAC	1 cut(s)	450
BslI	CCNNNNNNNGG	3 cut(s)	85, 728, 1349
BsmAI	GTCTC	2 cut(s)	508, 783
BsmFI	GGGAC	1 cut(s)	450
BsnI	GGCC	1 cut(s)	1589
BsoBI	CYCGRG	1 cut(s)	526
Bsp119I	TTCGAA	1 cut(s)	309
Bsp1286I	GDGCHC	2 cut(s)	533, 744
Bsp1720I	GCTNAGC	1 cut(s)	1874
Bsp68I	TCGCGA	1 cut(s)	1688
BspANI	GGCC	1 cut(s)	1589
BspCNI	CTCAG	6 cut(s)	106, 527, 679, 1428, 1804, 1887
BspFNI	CGCG	2 cut(s)	1432, 1688
BspLI	GGNNCC	2 cut(s)	672, 1794
BspMAI	CTGCAG	1 cut(s)	1258
BspMI	ACCTGC	1 cut(s)	458
BspOI	GCTAGC	1 cut(s)	806
BspPI	GGATC	6 cut(s)	665, 671, 678, 1117, 1241, 1334
BspT104I	TTCGAA	1 cut(s)	309
BspT107I	GGYRCC	1 cut(s)	1792
BsrBI	CCGCTC	3 cut(s)	154, 1550, 1737
BsrDI	GCAATG	1 cut(s)	199
BsrFI	RCCGGY	2 cut(s)	1592, 1867
BsrI	ACTGG	3 cut(s)	932, 1805, 1862
BssAI	RCCGGY	2 cut(s)	1592, 1867
BssECI	CCNNGG	3 cut(s)	187, 1098, 1163
BssNI	GRCGYC	1 cut(s)	891
BssSI	CACGAG	2 cut(s)	1382, 1465
Bst2BI	CACGAG	2 cut(s)	1382, 1465
Bst2UI	CCWGG	1 cut(s)	425
Bst4CI	ACNGT	4 cut(s)	36, 289, 350, 2024
Bst6I	CTCTTC	2 cut(s)	86, 250
BstACI	GRCGYC	1 cut(s)	891
BstAPI	GCANNNNNTGC	1 cut(s)	1532
BstBI	TTCGAA	1 cut(s)	309
BstC8I	GCNNGC	5 cut(s)	60, 804, 1086, 1739, 1869
BstDEI	CTNAG	7 cut(s)	93, 514, 687, 1016, 1436, 1812, 1874
BstDSI	CCRYGG	1 cut(s)	1163
BstEII	GGTNACC	1 cut(s)	350
BstF5I	GGATG	9 cut(s)	102, 146, 213, 509, 581, 614, 808, 1177, 1935
BstFNI	CGCG	2 cut(s)	1432, 1688
BstH2I	RGCGCY	2 cut(s)	1874, 1950
BstHHI	GCGC	3 cut(s)	1579, 1873, 1949
BstMAI	GTCTC	2 cut(s)	508, 783
BstMCI	CGRYCG	1 cut(s)	489
BstMWI	GCNNNNNNNGC	9 cut(s)	68, 515, 1076, 1085, 1094, 1532, 1541, 1801, 1868
BstNI	CCWGG	1 cut(s)	425
BstNSI	RCATGY	1 cut(s)	1646
BstPAI	GACNNNNGTC	1 cut(s)	1886
BstPI	GGTNACC	1 cut(s)	350
BstSCI	CCNGG	2 cut(s)	84, 423
BstSFI	CTRYAG	1 cut(s)	1254
BstUI	CGCG	2 cut(s)	1432, 1688
BstV1I	GCAGC	6 cut(s)	746, 1063, 1240, 1556, 1587, 1637
BstV2I	GAAGAC	1 cut(s)	1035
BstX2I	RGATCY	1 cut(s)	670
BstXI	CCANNNNNNTGG	1 cut(s)	939
BstYI	RGATCY	1 cut(s)	670
BsuI	GTATCC	1 cut(s)	304
BsuRI	GGCC	1 cut(s)	1589
BtgI	CCRYGG	1 cut(s)	1163
BtgZI	GCGATG	2 cut(s)	310, 1848
BtrI	CACGTC	1 cut(s)	1041
BtsCI	GGATG	9 cut(s)	102, 146, 213, 509, 581, 614, 808, 1177, 1935
BtuMI	TCGCGA	1 cut(s)	1688
BveI	ACCTGC	1 cut(s)	458
Cac8I	GCNNGC	5 cut(s)	60, 804, 1086, 1739, 1869
CaiI	CAGNNNCTG	1 cut(s)	1811
CfoI	GCGC	3 cut(s)	1579, 1873, 1949
Cfr10I	RCCGGY	2 cut(s)	1592, 1867
CseI	GACGC	4 cut(s)	880, 906, 1019, 1421
CsiI	ACCWGGT	1 cut(s)	423
Csp6I	GTAC	9 cut(s)	32, 862, 1048, 1176, 1303, 1360, 1515, 1537, 1933
CspAI	ACCGGT	1 cut(s)	1592
CspCI	CAANNNNNGTGG	2 cut(s)	1619, 1654
CviAII	CATG	9 cut(s)	356, 469, 567, 799, 1202, 1212, 1347, 1643, 1899
CviQI	GTAC	9 cut(s)	32, 862, 1048, 1176, 1303, 1360, 1515, 1537, 1933
DdeI	CTNAG	7 cut(s)	93, 514, 687, 1016, 1436, 1812, 1874
Eam1104I	CTCTTC	2 cut(s)	86, 250
EarI	CTCTTC	2 cut(s)	86, 250
Eco57I	CTGAAG	1 cut(s)	691
Eco88I	CYCGRG	1 cut(s)	526
Eco91I	GGTNACC	1 cut(s)	350
EcoO65I	GGTNACC	1 cut(s)	350
EcoRI	GAATTC	3 cut(s)	943, 1487, 1976
EcoRII	CCWGG	1 cut(s)	423
EcoT22I	ATGCAT	1 cut(s)	1921
FaeI	CATG	9 cut(s)	359, 472, 570, 802, 1205, 1215, 1350, 1646, 1902
FaqI	GGGAC	1 cut(s)	450
FatI	CATG	9 cut(s)	355, 468, 566, 798, 1201, 1211, 1346, 1642, 1898
FauI	CCCGC	4 cut(s)	76, 159, 1730, 2003
FbaI	TGATCA	1 cut(s)	559
FblI	GTMKAC	1 cut(s)	1721
Fnu4HI	GCNGC	8 cut(s)	735, 1077, 1254, 1473, 1545, 1548, 1601, 1626
FokI	GGATG	9 cut(s)	89, 133, 200, 496, 568, 601, 795, 1184, 1942
Fsp4HI	GCNGC	8 cut(s)	735, 1077, 1254, 1473, 1545, 1548, 1601, 1626
FspBI	CTAG	5 cut(s)	803, 1064, 1071, 1842, 1850
FspI	TGCGCA	1 cut(s)	1578
GlaI	GCGC	3 cut(s)	1578, 1872, 1948
GluI	GCNGC	8 cut(s)	735, 1077, 1254, 1473, 1545, 1548, 1601, 1626
HaeII	RGCGCY	2 cut(s)	1874, 1950
HaeIII	GGCC	1 cut(s)	1589
HapII	CCGG	4 cut(s)	86, 668, 1593, 1868
HgaI	GACGC	4 cut(s)	880, 906, 1019, 1421
HhaI	GCGC	3 cut(s)	1579, 1873, 1949
Hin1I	GRCGYC	1 cut(s)	891
Hin1II	CATG	9 cut(s)	359, 472, 570, 802, 1205, 1215, 1350, 1646, 1902
Hin6I	GCGC	3 cut(s)	1577, 1871, 1947
HinP1I	GCGC	3 cut(s)	1577, 1871, 1947
HincII	GTYRAC	4 cut(s)	624, 1009, 1482, 1722
HindII	GTYRAC	4 cut(s)	624, 1009, 1482, 1722
HindIII	AAGCTT	3 cut(s)	824, 1371, 1966
HpaI	GTTAAC	1 cut(s)	624
HpaII	CCGG	4 cut(s)	86, 668, 1593, 1868
HphI	GGTGA	6 cut(s)	313, 344, 439, 579, 911, 1277
Hpy166II	GTNNAC	7 cut(s)	99, 624, 1009, 1220, 1482, 1722, 1855
Hpy188III	TCNNGA	7 cut(s)	674, 698, 1001, 1115, 1144, 1508, 1687
Hpy8I	GTNNAC	7 cut(s)	99, 624, 1009, 1220, 1482, 1722, 1855
Hpy99I	CGWCG	7 cut(s)	26, 293, 878, 896, 922, 1002, 1985
HpyAV	CCTTC	5 cut(s)	126, 133, 178, 1684, 1937
HpyCH4III	ACNGT	4 cut(s)	36, 289, 350, 2024
HpyCH4IV	ACGT	2 cut(s)	620, 1040
HpyF10VI	GCNNNNNNNGC	9 cut(s)	68, 515, 1076, 1085, 1094, 1532, 1541, 1801, 1868
HpyF3I	CTNAG	7 cut(s)	93, 514, 687, 1016, 1436, 1812, 1874
HpySE526I	ACGT	2 cut(s)	620, 1040
Hsp92I	GRCGYC	1 cut(s)	891
Hsp92II	CATG	9 cut(s)	359, 472, 570, 802, 1205, 1215, 1350, 1646, 1902
HspAI	GCGC	3 cut(s)	1577, 1871, 1947
KroI	GCCGGC	1 cut(s)	1867
KroNI	GCCGGC	1 cut(s)	1869
Ksp22I	TGATCA	1 cut(s)	559
KspAI	GTTAAC	1 cut(s)	624
LmnI	GCTCC	1 cut(s)	159
Lsp1109I	GCAGC	6 cut(s)	746, 1063, 1240, 1556, 1587, 1637
LweI	GCATC	5 cut(s)	518, 879, 1430, 1564, 1871
MabI	ACCWGGT	1 cut(s)	423
MaeI	CTAG	5 cut(s)	803, 1064, 1071, 1842, 1850
MaeII	ACGT	2 cut(s)	620, 1040
MaeIII	GTNAC	7 cut(s)	350, 385, 716, 934, 1225, 1807, 1888
MbiI	CCGCTC	3 cut(s)	154, 1550, 1737
MboII	GAAGA	7 cut(s)	73, 118, 163, 237, 1035, 1969, 1985
MflI	RGATCY	1 cut(s)	670
MhlI	GDGCHC	2 cut(s)	533, 744
MluI	ACGCGT	1 cut(s)	1430
MlyI	GAGTC	2 cut(s)	605, 1896
MmeI	TCCRAC	5 cut(s)	390, 432, 858, 931, 1149
Mph1103I	ATGCAT	1 cut(s)	1921
MreI	CGCCGGCG	1 cut(s)	1867
MroNI	GCCGGC	1 cut(s)	1867
MseI	TTAA	4 cut(s)	623, 630, 858, 1530
MspA1I	CMGCKG	2 cut(s)	737, 1253
MspI	CCGG	4 cut(s)	86, 668, 1593, 1868
MspR9I	CCNGG	2 cut(s)	86, 425
MvaI	CCWGG	1 cut(s)	425
MvnI	CGCG	2 cut(s)	1432, 1688
MwoI	GCNNNNNNNGC	9 cut(s)	68, 515, 1076, 1085, 1094, 1532, 1541, 1801, 1868
NaeI	GCCGGC	1 cut(s)	1869
NciI	CCSGG	1 cut(s)	86
NgoMIV	GCCGGC	1 cut(s)	1867
NheI	GCTAGC	1 cut(s)	802
NlaIII	CATG	9 cut(s)	359, 472, 570, 802, 1205, 1215, 1350, 1646, 1902
NlaIV	GGNNCC	2 cut(s)	672, 1794
NmeAIII	GCCGAG	3 cut(s)	168, 485, 1123
NmuCI	GTSAC	4 cut(s)	350, 385, 934, 1888
NruI	TCGCGA	1 cut(s)	1688
NsbI	TGCGCA	1 cut(s)	1578
NsiI	ATGCAT	1 cut(s)	1921
NspI	RCATGY	1 cut(s)	1646
NspV	TTCGAA	1 cut(s)	309
PaeR7I	CTCGAG	1 cut(s)	526
PaqCI	CACCTGC	1 cut(s)	458
PcsI	WCGNNNNNNNCGW	3 cut(s)	27, 195, 549
PdiI	GCCGGC	1 cut(s)	1869
PfoI	TCCNGGA	1 cut(s)	84
PinAI	ACCGGT	1 cut(s)	1592
PkrI	GCNGC	8 cut(s)	736, 1078, 1255, 1474, 1546, 1549, 1602, 1627
PleI	GAGTC	2 cut(s)	605, 1895
PpsI	GAGTC	2 cut(s)	605, 1895
PshAI	GACNNNNGTC	1 cut(s)	1886
Psp6I	CCWGG	1 cut(s)	423
PspEI	GGTNACC	1 cut(s)	350
PspGI	CCWGG	1 cut(s)	423
PspN4I	GGNNCC	2 cut(s)	672, 1794
PspXI	VCTCGAGB	1 cut(s)	526
PstI	CTGCAG	1 cut(s)	1258
PstNI	CAGNNNCTG	1 cut(s)	1811
PsuI	RGATCY	1 cut(s)	670
PvuII	CAGCTG	2 cut(s)	737, 1253
RruI	TCGCGA	1 cut(s)	1688
RsaI	GTAC	9 cut(s)	33, 863, 1049, 1177, 1304, 1361, 1516, 1538, 1934
RsaNI	GTAC	9 cut(s)	32, 862, 1048, 1176, 1303, 1360, 1515, 1537, 1933
SalI	GTCGAC	1 cut(s)	1720
SaqAI	TTAA	4 cut(s)	623, 630, 858, 1530
SatI	GCNGC	8 cut(s)	735, 1077, 1254, 1473, 1545, 1548, 1601, 1626
SchI	GAGTC	2 cut(s)	605, 1896
ScrFI	CCNGG	2 cut(s)	86, 425
SduI	GDGCHC	2 cut(s)	533, 744
SexAI	ACCWGGT	1 cut(s)	423
SfaNI	GCATC	5 cut(s)	518, 879, 1430, 1564, 1871
SfcI	CTRYAG	1 cut(s)	1254
Sfr274I	CTCGAG	1 cut(s)	526
SfuI	TTCGAA	1 cut(s)	309
SgrAI	CRCCGGYG	1 cut(s)	1867
SlaI	CTCGAG	1 cut(s)	526
SmlI	CTYRAG	5 cut(s)	526, 821, 1144, 1368, 1583
SmoI	CTYRAG	5 cut(s)	526, 821, 1144, 1368, 1583
SpeI	ACTAGT	1 cut(s)	1063
SspMI	CTAG	5 cut(s)	803, 1064, 1071, 1842, 1850
StyD4I	CCNGG	2 cut(s)	84, 423
TaaI	ACNGT	4 cut(s)	36, 289, 350, 2024
TaiI	ACGT	2 cut(s)	623, 1043
TaqII	GACCGA	1 cut(s)	503
TatI	WGTACW	2 cut(s)	861, 1359
TauI	GCSGC	2 cut(s)	1475, 1550
Tru1I	TTAA	4 cut(s)	623, 630, 858, 1530
Tru9I	TTAA	4 cut(s)	623, 630, 858, 1530
TseFI	GTSAC	4 cut(s)	350, 385, 934, 1888
TseI	GCWGC	6 cut(s)	734, 1076, 1253, 1544, 1600, 1625
Tsp45I	GTSAC	4 cut(s)	350, 385, 934, 1888
TspDTI	ATGAA	3 cut(s)	529, 1011, 1283
TspGWI	ACGGA	4 cut(s)	937, 1206, 1312, 1907
XapI	RAATTY	7 cut(s)	596, 635, 943, 1487, 1617, 1668, 1976
XceI	RCATGY	1 cut(s)	1646
XhoI	CTCGAG	1 cut(s)	526
XmiI	GTMKAC	1 cut(s)	1721
XspI	CTAG	5 cut(s)	803, 1064, 1071, 1842, 1850
Zsp2I	ATGCAT	1 cut(s)	1921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.