pycom17g27190

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000677
Physical Location & Seq
Forward (+)
25489974 .. 25490334
361 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 201 bp
ATGGAGTTTTGCCGGAAGAAGCTGCCGCACTTCATGGTGCCCAAAACGGTGGTGTTTAAGGATGAGCTTCCCAAGACTTCCACCGGAAAGATTCAGAAGTTTTTGCTGAGGGAGATTGCCAGAGGGATTGCGCTGTCTTCCTTATTTTCTCCTCCGCCGCCGCCGGCGTCGCTGACGACGAATAGGATGTGTATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

67

Amino Acids

7.43

Weight (kDa)

10.15

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 2 - 30 1.7e-06 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 37
AciI CCGC 4 cut(s) 26, 155, 158, 161
AcyI GRCGYC 1 cut(s) 167
AluBI AGCT 2 cut(s) 22, 67
AluI AGCT 2 cut(s) 22, 67
ApeKI GCWGC 1 cut(s) 22
AspLEI GCGC 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 42
BanI GGYRCC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 129
BbvCI CCTCAGC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 9
BisI GCNGC 4 cut(s) 23, 26, 158, 161
BlsI GCNGC 4 cut(s) 24, 27, 159, 162
BmiI GGNNCC 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 129
Bpu10I CCTNAGC 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 83
Bse118I RCCGGY 1 cut(s) 163
BseGI GGATG 2 cut(s) 67, 192
BseMII CTCAG 1 cut(s) 98
BseRI GAGGAG 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 42
BseXI GCAGC 1 cut(s) 9
BshNI GGYRCC 1 cut(s) 37
BsiSI CCGG 3 cut(s) 13, 84, 164
Bsp1286I GDGCHC 1 cut(s) 42
BspACI CCGC 4 cut(s) 26, 155, 158, 161
BspCNI CTCAG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 163
BssNI GRCGYC 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 49
BstACI GRCGYC 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 1 cut(s) 107
BstF5I GGATG 2 cut(s) 67, 192
BstHHI GCGC 1 cut(s) 133
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 42
BstV1I GCAGC 1 cut(s) 9
BstV2I GAAGAC 1 cut(s) 129
BstXI CCANNNNNNTGG 1 cut(s) 49
BtsCI GGATG 2 cut(s) 67, 192
Cac8I GCNNGC 1 cut(s) 165
CfoI GCGC 1 cut(s) 133
Cfr10I RCCGGY 1 cut(s) 163
CseI GACGC 1 cut(s) 156
CviAII CATG 1 cut(s) 34
CviJI RGCY 3 cut(s) 22, 67, 197
CviKI_1 RGCY 3 cut(s) 22, 67, 197
DdeI CTNAG 1 cut(s) 107
EciI GGCGGA 1 cut(s) 144
FaeI CATG 1 cut(s) 37
FaiI YATR 2 cut(s) 35, 194
FatI CATG 1 cut(s) 33
Fnu4HI GCNGC 4 cut(s) 23, 26, 158, 161
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 4 cut(s) 23, 26, 158, 161
GlaI GCGC 1 cut(s) 132
GluI GCNGC 4 cut(s) 23, 26, 158, 161
HapII CCGG 3 cut(s) 13, 84, 164
HgaI GACGC 1 cut(s) 156
HhaI GCGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 167
Hin1II CATG 1 cut(s) 37
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HinfI GANTC 1 cut(s) 91
HpaII CCGG 3 cut(s) 13, 84, 164
Hpy188I TCNGA 1 cut(s) 96
Hpy99I CGWCG 2 cut(s) 172, 181
HpyCH4III ACNGT 1 cut(s) 49
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 1 cut(s) 107
Hsp92I GRCGYC 1 cut(s) 167
Hsp92II CATG 1 cut(s) 37
HspAI GCGC 1 cut(s) 131
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
LpnPI CCDG 4 cut(s) 26, 97, 133, 177
Lsp1109I GCAGC 1 cut(s) 9
MboII GAAGA 2 cut(s) 28, 129
MhlI GDGCHC 1 cut(s) 42
MnlI CCTC 3 cut(s) 102, 116, 162
MreI CGCCGGCG 1 cut(s) 163
MroNI GCCGGC 1 cut(s) 163
MseI TTAA 1 cut(s) 57
MspI CCGG 3 cut(s) 13, 84, 164
MwoI GCNNNNNNNGC 1 cut(s) 169
NaeI GCCGGC 1 cut(s) 165
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 176
PdiI GCCGGC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 91
PkrI GCNGC 4 cut(s) 24, 27, 159, 162
PspN4I GGNNCC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 4 cut(s) 23, 26, 158, 161
SduI GDGCHC 1 cut(s) 42
SetI ASST 2 cut(s) 24, 69
SgeI CNNG 6 cut(s) 25, 46, 85, 96, 132, 176
SgrAI CRCCGGYG 1 cut(s) 163
SsiI CCGC 4 cut(s) 26, 155, 158, 161
TaaI ACNGT 1 cut(s) 49
TauI GCSGC 3 cut(s) 28, 160, 163
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TseI GCWGC 1 cut(s) 22
TspDTI ATGAA 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.