Rroxscaffold_3G00225360
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000076
Physical Location & Seq
Forward (+)
8631324 .. 8632058
735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225360.1

Sequence Viewer

Length: 723 bp
ATGCAGCAAGAGTTTGCAGTGGAGGAGAGCAGAGGCAATCAGCTTCCTGGAGAGAGGTTCTGCCCCATGGAGGACGAACTAGTTCTCTTCTACCTTAAGCCCATGTTGAGTGGAGAGAAGGTGCCCGGTAGAAACCGCGTGGTGTTCGACTGCGACCTCTACGGTCACCAAGAACCTTGGGAGATATGGGAGGCCTACAAGACCAGAAGACTAAACGACTTGAGGCTCAACAAGGACATCTACTTCTTCACCCAACACAAGACGATGAGTTCCATAGACACACGCATACGCCGGACTGTTGGCAATGGCACCTGGAAGGGCGACGACTCCGGTAAGCCAGTAAAATCTTTGGAGACTGGTCGTGTGAAAAGACTTACTTACAAGAATGAAGCATCAGAGCACAACGATTGTTGGATCTTGTATGAGTTCTACCTTGATCGATCACTCAGAGACGAGAAACAAAAGGTGAAAGACTATGTTCTTTGTCTGCTAAGAAAAAATGGTGAACCCAAAACCAAGATAAAAAAGAAGAGAAAGCAACGAGAAGAGGAAGAGGTTCTTGAAAATAATTGTGCCTGTGATGATGGAGAGAACACAAACAAGGAGCAAGAAGAGTTGCTTGAGCCTCAAGCGAAGCGACAACGATATGTTCCATCCATTGATAATGCACTAGTACCAATGCCATCAGAAGAACCCTCAGAAGCTGAAGCAATTGACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

240

Amino Acids

28.32

Weight (kDa)

5.53

Isoelectric Point (pI)

59.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 16 - 144 8.1e-21 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53610 AT1G53620
fragaria_vesca FvH4_3g16890 FvH4_3g16900 FvH4_3g16910 FvH4_3g17012 FvH4_3g18170 FvH4_3g18460 FvH4_3g18930 FvH4_3g18940 FvH4_3g25560 FvH4_4g12410 FvH4_5g05980 FvH4_5g24860 FvH4_5g25360 FvH4_5g25510 FvH4_5g27470 FvH4_5g27620 FvH4_5g27760 FvH4_5g29460 FvH4_6g04191 FvH4_6g39190 FvH4_6g39200 FvH4_6g39580 FvH4_6g39600 FvH4_7g11470
malus_domestica MD00G1154700.v1.1 MD00G1154800.v1.1 MD00G1155000.v1.1 MD03G1255000.v1.1 MD03G1255100.v1.1 MD03G1255200.v1.1 MD03G1255300.v1.1 MD06G1030300.v1.1 MD11G1219300.v1.1 MD11G1264900.v1.1
prunus_persica Prupe.1G575600_v2.0.a1 Prupe.3G065000_v2.0.a1 Prupe.3G111400_v2.0.a1 Prupe.4G151300_v2.0.a1 Prupe.4G151900_v2.0.a1 Prupe.4G153700_v2.0.a1 Prupe.4G154000_v2.0.a1 Prupe.4G230600_v2.0.a1 Prupe.4G230900_v2.0.a1 Prupe.4G231000_v2.0.a1 Prupe.4G246100_v2.0.a1 Prupe.4G255500_v2.0.a1 Prupe.6G133600_v2.0.a1 Prupe.6G134000_v2.0.a1 Prupe.I005300_v2.0.a1
pyrus_communis pycom03g14030 pycom03g15340
rosa_chinensis RchiOBHm_Chr3g0452871 RchiOBHm_Chr5g0056721 RchiOBHm_Chr6g0271921 RchiOBHm_Chr7g0211251 RchiOBHm_Chr7g0231191
rosa_laevigata RLG00000000989 RLG00000001070 RLG00000001076 RLG00000001103 RLG00000001116 RLG00000001118 RLG00000001936 RLG00000001949 RLG00000002610 RLG00000002710 RLG00000003013 RLG00000013562 RLG00000013567 RLG00000018805 RLG00000020667 RLG00000020716 RLG00000020717 RLG00000025564 RLG00000028522 RLG00000030016 RLG00000033070 RLG00000033077 RLG00000033081 RLG00000033086 RLG00000033235 RLG00000033291 RLG00000033337 RLG00000033338 RLG00000033340 RLG00000033345 RLG00000033346 RLG00000033874 RLG00000033885 RLG00000034356 RLG00000034411 RLG00000034414
rosa_multiflora Rmu_co8273153.1_g000001 Rmu_co8288493.1_g000001 Rmu_co8293081.1_g000001 Rmu_co8429197.1_g000001 Rmu_sc0000055.1_g000007 Rmu_sc0000218.1_g000011 Rmu_sc0000533.1_g000061 Rmu_sc0000755.1_g000007 Rmu_sc0000758.1_g000005 Rmu_sc0000898.1_g000011 Rmu_sc0000898.1_g000025 Rmu_sc0001561.1_g000008 Rmu_sc0001571.1_g000004 Rmu_sc0002292.1_g000005 Rmu_sc0002292.1_g000006 Rmu_sc0002308.1_g000010 Rmu_sc0002449.1_g000010 Rmu_sc0002449.1_g000011 Rmu_sc0002536.1_g000004 Rmu_sc0002536.1_g000005 Rmu_sc0002536.1_g000015 Rmu_sc0003238.1_g000014 Rmu_sc0003545.1_g000015 Rmu_sc0003720.1_g000009 Rmu_sc0003878.1_g000040 Rmu_sc0003878.1_g000041 Rmu_sc0003878.1_g000047 Rmu_sc0003878.1_g000048 Rmu_sc0003972.1_g000001 Rmu_sc0004322.1_g000008 Rmu_sc0004379.1_g000014 Rmu_sc0005478.1_g000002 Rmu_sc0006240.1_g000008 Rmu_sc0006847.1_g000022 Rmu_sc0007753.1_g000022 Rmu_sc0007782.1_g000001 Rmu_sc0008328.1_g000002 Rmu_sc0008526.1_g000005 Rmu_sc0008835.1_g000013 Rmu_sc0014005.1_g000009 Rmu_sc0014478.1_g000004 Rmu_sc0015060.1_g000021 Rmu_sc0016364.1_g000001 Rmu_sc0016727.1_g000004 Rmu_sc0020600.1_g000008 Rmu_sc0022743.1_g000003 Rmu_sc0032116.1_g000002 Rmu_sc0033519.1_g000005 Rmu_sc0042823.1_g000001 Rmu_ssc0000134.1_g000011
rosa_roxburghii Rroxscaffold_1G00029360 Rroxscaffold_1G00034880 Rroxscaffold_1G00034930 Rroxscaffold_1G00034970 Rroxscaffold_1G00042100 Rroxscaffold_1G00049130 Rroxscaffold_1G00049660 Rroxscaffold_1G00051410 Rroxscaffold_2G00094840 Rroxscaffold_2G00094850 Rroxscaffold_2G00095300 Rroxscaffold_3G00224990 Rroxscaffold_3G00225050 Rroxscaffold_3G00225080 Rroxscaffold_3G00225360 Rroxscaffold_3G00235370 Rroxscaffold_3G00243820 Rroxscaffold_3G00243860 Rroxscaffold_3G00243920 Rroxscaffold_3G00244920 Rroxscaffold_4G00305330 Rroxscaffold_5G00373100 Rroxscaffold_6G00407840 Rroxscaffold_6G00422000 Rroxscaffold_6G00429880 Rroxscaffold_7G00186180
rosa_rugosa Rorug01G0120600 Rorug01G0127200 Rorug01G0128500 Rorug01G0206000 Rorug02G0251400 Rorug02G0420800 Rorug02G0436800 Rorug02G0441000 Rorug02G0643100 Rorug03G0339200 Rorug04G0168000 Rorug04G0168100 Rorug05G0104500 Rorug05G0105300 Rorug05G0105700 Rorug05G0105800 Rorug05G0118900 Rorug05G0175700 Rorug05G0228200 Rorug05G0229000 Rorug05G0229500 Rorug06G0064300 Rorug07G0124700 Rorug07G0156100 Rorug07G0156700 Rorug07G0158100 Rorug07G0219600 Rorug07G0273400 Rorug07G0291800 Rorug07G0294300 Rorug07G0297400 Rorug07G0297800
rosa_samantha Rh1BG189400 Rh1CG103600 Rh1CG142100 Rh1CG208100 Rh1DG209200 Rh2CG296600 Rh2CG301600 Rh2CG485100 Rh2CG489800 Rh2CG490000 Rh2DG333700 Rh2DG526600 Rh2DG526800 Rh3BG048500 Rh3CG047100 Rh3DG048100 Rh5AG198800 Rh5AG211300 Rh5AG213900 Rh5AG217700 Rh5AG221800 Rh5AG225800 Rh5AG262500 Rh5AG309600 Rh5BG210200 Rh5CG216300 Rh5CG217100 Rh5CG217500 Rh5CG233900 Rh5CG236800 Rh5CG239600 Rh5CG241200 Rh5CG244900 Rh5CG245100 Rh5CG254600 Rh5CG299700 Rh5CG343400 Rh5CG343600 Rh5CG406800 Rh5DG215100 Rh5DG215200 Rh5DG220900 Rh5DG222800 Rh5DG226600 Rh5DG226700 Rh5DG226800 Rh5DG231600 Rh5DG273600 Rh5DG327700 Rh5DG328300 Rh6BG185200 Rh6DG174700 Rh7AG257100 Rh7AG285100 Rh7AG293000 Rh7AG294700 Rh7AG296700 Rh7AG297200 Rh7AG298400 Rh7AG306700 Rh7AG362600 Rh7AG379100 Rh7AG449400 Rh7AG451800 Rh7AG453400 Rh7AG453500 Rh7AG459000 Rh7BG251000 Rh7BG275900 Rh7BG276100 Rh7BG276800 Rh7BG285400 Rh7BG353800 Rh7BG400900 Rh7BG418600 Rh7BG421100 Rh7BG424000 Rh7BG424300 Rh7BG424400 Rh7DG288700
rosa_wichuraiana Rw0G000390 Rw0G010750 Rw0G015930 Rw0G020680 Rw0G023760 Rw1G008930 Rw1G012360 Rw1G019040 Rw2G025060 Rw2G041400 Rw2G041420 Rw3G003610 Rw5G018050 Rw5G018130 Rw5G019340 Rw5G019550 Rw5G020700 Rw5G024510 Rw5G036060 Rw6G015630 Rw7G021960 Rw7G024340 Rw7G024900 Rw7G024940 Rw7G025030 Rw7G025280 Rw7G030800 Rw7G032090 Rw7G037610 Rw7G038010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 121, 308
AccII CGCG 1 cut(s) 138
AciI CCGC 1 cut(s) 136
AclWI GGATC 1 cut(s) 422
AfaI GTAC 1 cut(s) 675
AfiI CCNNNNNNNGG 1 cut(s) 70
AflII CTTAAG 1 cut(s) 95
AgsI TTSAA 1 cut(s) 563
AhlI ACTAGT 2 cut(s) 79, 670
AjnI CCWGG 2 cut(s) 46, 311
AluBI AGCT 2 cut(s) 43, 704
AluI AGCT 2 cut(s) 43, 704
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 2 cut(s) 347, 444
AlwI GGATC 1 cut(s) 422
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 4
Asp700I GAANNNNTTC 2 cut(s) 81, 555
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 4 cut(s) 158, 241, 478, 515
BaeGI GKGCMC 1 cut(s) 126
BanI GGYRCC 2 cut(s) 121, 308
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 578, 661, 691
BciT130I CCWGG 2 cut(s) 48, 313
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 347, 444
BcuI ACTAGT 2 cut(s) 79, 670
BfaI CTAG 2 cut(s) 80, 671
BfrI CTTAAG 1 cut(s) 95
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 3 cut(s) 48, 126, 313
BmiI GGNNCC 2 cut(s) 123, 310
BmrFI CCNGG 3 cut(s) 48, 126, 313
BmsI GCATC 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 69
BpuEI CTTGAG 3 cut(s) 241, 612, 641
BpuMI CCSGG 1 cut(s) 126
Bsa29I ATCGAT 1 cut(s) 439
BsaJI CCNNGG 2 cut(s) 66, 176
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse1I ACTGG 2 cut(s) 338, 361
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 2 cut(s) 48, 313
BseCI ATCGAT 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 66, 176
BseGI GGATG 1 cut(s) 653
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 2 cut(s) 460, 711
BseNI ACTGG 2 cut(s) 338, 361
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 2 cut(s) 121, 308
BshVI ATCGAT 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 3 cut(s) 126, 292, 330
BslI CCNNNNNNNGG 1 cut(s) 70
BsmAI GTCTC 2 cut(s) 347, 444
BsmBI CGTCTC 1 cut(s) 444
BsnI GGCC 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 126, 402
Bsp143I GATC 3 cut(s) 414, 436, 440
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 1 cut(s) 136
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 459, 710
BspDI ATCGAT 1 cut(s) 439
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 123, 310
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 2 cut(s) 121, 308
BspTI CTTAAG 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 2 cut(s) 338, 361
BssECI CCNNGG 2 cut(s) 66, 176
BssMI GATC 3 cut(s) 414, 436, 440
BssT1I CCWWGG 2 cut(s) 66, 176
Bst2UI CCWGG 2 cut(s) 48, 313
Bst4CI ACNGT 2 cut(s) 164, 298
Bst6I CTCTTC 5 cut(s) 92, 524, 540, 546, 606
BstAFI CTTAAG 1 cut(s) 95
BstDEI CTNAG 3 cut(s) 446, 491, 697
BstDSI CCRYGG 1 cut(s) 66
BstEII GGTNACC 1 cut(s) 164
BstF5I GGATG 1 cut(s) 653
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 3 cut(s) 417, 439, 443
BstMAI GTCTC 2 cut(s) 347, 444
BstMBI GATC 3 cut(s) 414, 436, 440
BstNI CCWGG 2 cut(s) 48, 313
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 3 cut(s) 46, 124, 311
BstSLI GKGCMC 1 cut(s) 126
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
Bsu15I ATCGAT 1 cut(s) 439
BsuRI GGCC 1 cut(s) 194
BsuTUI ATCGAT 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 1 cut(s) 653
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 24
CaiI CAGNNNCTG 1 cut(s) 704
ClaI ATCGAT 1 cut(s) 439
Csp6I GTAC 1 cut(s) 674
CviAII CATG 2 cut(s) 67, 103
CviJI RGCY 7 cut(s) 43, 100, 194, 226, 337, 625, 704
CviKI_1 RGCY 7 cut(s) 43, 100, 194, 226, 337, 625, 704
CviQI GTAC 1 cut(s) 674
DdeI CTNAG 3 cut(s) 446, 491, 697
DpnI GATC 3 cut(s) 416, 438, 442
DpnII GATC 3 cut(s) 414, 436, 440
Eam1104I CTCTTC 5 cut(s) 92, 524, 540, 546, 606
EarI CTCTTC 5 cut(s) 92, 524, 540, 546, 606
Eco130I CCWWGG 2 cut(s) 66, 176
Eco147I AGGCCT 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 164
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 46, 311
EcoT14I CCWWGG 2 cut(s) 66, 176
ErhI CCWWGG 2 cut(s) 66, 176
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 2 cut(s) 70, 106
FaiI YATR 8 cut(s) 68, 104, 187, 275, 287, 423, 477, 648
FalI AAGNNNNNCTT 6 cut(s) 361, 393, 543, 575, 603, 635
FatI CATG 2 cut(s) 66, 102
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 1 cut(s) 640
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 2 cut(s) 80, 671
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 1 cut(s) 194
HapII CCGG 3 cut(s) 126, 292, 330
Hin1II CATG 2 cut(s) 70, 106
HinfI GANTC 1 cut(s) 326
HpaII CCGG 3 cut(s) 126, 292, 330
HphI GGTGA 4 cut(s) 158, 241, 478, 515
Hpy166II GTNNAC 1 cut(s) 506
Hpy188I TCNGA 4 cut(s) 397, 449, 688, 700
Hpy188III TCNNGA 1 cut(s) 560
Hpy8I GTNNAC 1 cut(s) 506
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 2 cut(s) 112, 310
HpyCH4III ACNGT 2 cut(s) 164, 298
HpyCH4V TGCA 3 cut(s) 4, 17, 668
HpyF3I CTNAG 3 cut(s) 446, 491, 697
Hsp92II CATG 2 cut(s) 70, 106
Kzo9I GATC 3 cut(s) 414, 436, 440
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 401
MaeI CTAG 2 cut(s) 80, 671
MaeIII GTNAC 1 cut(s) 164
MalI GATC 3 cut(s) 416, 438, 442
MboI GATC 3 cut(s) 414, 436, 440
MboII GAAGA 8 cut(s) 79, 219, 238, 541, 557, 563, 623, 701
MfeI CAATTG 1 cut(s) 711
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 2 cut(s) 126, 402
MluCI AATT 2 cut(s) 568, 711
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 392
MroXI GAANNNNTTC 2 cut(s) 81, 555
MseI TTAA 2 cut(s) 96, 721
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 3 cut(s) 126, 292, 330
MspR9I CCNGG 3 cut(s) 48, 126, 313
MunI CAATTG 1 cut(s) 711
MvaI CCWGG 2 cut(s) 48, 313
MvnI CGCG 1 cut(s) 138
NciI CCSGG 1 cut(s) 126
NcoI CCATGG 1 cut(s) 66
NdeII GATC 3 cut(s) 414, 436, 440
NlaIII CATG 2 cut(s) 70, 106
NlaIV GGNNCC 2 cut(s) 123, 310
NmuCI GTSAC 1 cut(s) 164
PceI AGGCCT 1 cut(s) 194
PdmI GAANNNNTTC 2 cut(s) 81, 555
PfoI TCCNGGA 1 cut(s) 46
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 320
PpsI GAGTC 1 cut(s) 320
Psp6I CCWGG 2 cut(s) 46, 311
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 2 cut(s) 46, 311
PspN4I GGNNCC 2 cut(s) 123, 310
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 414
RsaI GTAC 1 cut(s) 675
RsaNI GTAC 1 cut(s) 674
SaqAI TTAA 2 cut(s) 96, 721
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 3 cut(s) 414, 436, 440
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 3 cut(s) 48, 126, 313
SduI GDGCHC 2 cut(s) 126, 402
SfaNI GCATC 1 cut(s) 401
SmlI CTYRAG 4 cut(s) 95, 220, 620, 627
SmoI CTYRAG 4 cut(s) 95, 220, 620, 627
SpeI ACTAGT 2 cut(s) 79, 670
Sse9I AATT 2 cut(s) 568, 711
SseBI AGGCCT 1 cut(s) 194
SsiI CCGC 1 cut(s) 136
SspMI CTAG 2 cut(s) 80, 671
StuI AGGCCT 1 cut(s) 194
StyD4I CCNGG 3 cut(s) 46, 124, 311
StyI CCWWGG 2 cut(s) 66, 176
TaaI ACNGT 2 cut(s) 164, 298
TaqI TCGA 2 cut(s) 147, 439
TasI AATT 2 cut(s) 568, 711
Tru1I TTAA 2 cut(s) 96, 721
Tru9I TTAA 2 cut(s) 96, 721
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 402
TspRI CASTG 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 95
XmnI GAANNNNTTC 2 cut(s) 81, 555
XspI CTAG 2 cut(s) 80, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.