Rroxscaffold_5G00353980

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000557
Physical Location & Seq
Forward (+)
32143055 .. 32147930
4876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353980.1

Sequence Viewer

Length: 828 bp
ATGTTTGAAGTTCGACGGTTAAGGATAATCGTAGTGTTTGTGTTCGAGGACGGGGATGTCCGAAGTTCAATGGTTAAGGATAACCAAAACTTGAGGTTGATTGTTTTATTGAGTGTTTGTAGGACCCAAGTGGGGTGTCGTGATTTGATGATTGTGTTAATGTGTTTCCTCGTGATTCCCTTTGATTTGGAGTGTGATCCTTGCCAATTAAAGGGTATTAGTCACCCCAATGAACCCAGAGCCAAGCTTGTGGTTGATCATTCTTGCAGGCATGCTTGCGACGCAGGGCATTGCATATATATAGGAGTGGTCTACTTGGTGATGATCACAGCATCGCCCAATCTAGAGGTTCACGAGCTGGATGAGCTCCCGGAGCGGTGGCGGAGGTTCAAGCTGGCTTGGCTTTGTAAGCAGCTGCCGTCGCATAATTCCGGGGCTTTGATTCGGATTCTCAATGCCCAGAAGAAGTGGATGAGTCAAGAGGACGCCGAGAACTCTGGTATTGTTAACTATAGAAGGTTCTTCAACAATGGCTTGCTTGAGGCTAGGATAGACTCCACGAGAGGAGACATCACGCCTTCCTTGGTCTGGTTTGAAGTCAACTATGGCCTTCTTATGCTCTTCGGGAAGATAGAGATTGTTGAGTGCATAAAAGAGATGGGACAGACGGTGCTTCCCATTTTCTATAATGTGGATCCATCAAATATACGGAAACAAACGGGAAGCTTTGCAGAAGCTTTTGATGCACATGAAGAACACTTCAAGGATAACTTAGAAAAAGTGCAAAGGTGGAGAGCTGCTTTGACTGAAGTGGCCAATCTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

275

Amino Acids

31.68

Weight (kDa)

6.26

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 212 - 273 1.8e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10320 AT1G10320
fragaria_vesca FvH4_1g01270 FvH4_5g28020
malus_domestica MD05G1262900.v1.1 MD05G1263000.v1.1 MD15G1442200.v1.1 MD15G1442300.v1.1
prunus_persica Prupe.1G585000_v2.0.a1
pyrus_communis pycom05g24740 pycom15g39000
rosa_chinensis RchiOBHm_Chr1g0376021 RchiOBHm_Chr2g0152801 RchiOBHm_Chr6g0272511 RchiOBHm_Chr6g0272531 RchiOBHm_Chr6g0289931 RchiOBHm_Chr6g0289941 RchiOBHm_Chr6g0302241 RchiOBHm_Chr7g0221381 RchiOBHm_Chr7g0221391 RchiOBHm_Chr7g0221411
rosa_laevigata RLG00000000942 RLG00000002158 RLG00000013669 RLG00000013670
rosa_multiflora Rmu_co8352637.1_g000001 Rmu_co8463189.1_g000001 Rmu_sc0000092.1_g000005 Rmu_sc0001575.1_g000019 Rmu_sc0001579.1_g000007 Rmu_sc0002847.1_g000017 Rmu_sc0009283.1_g000001 Rmu_sc0018146.1_g000002 Rmu_sc0026927.1_g000002 Rmu_sc0026927.1_g000003 Rmu_sc0031601.1_g000001
rosa_roxburghii Rroxscaffold_3G00238370 Rroxscaffold_5G00353980 Rroxscaffold_6G00399580 Rroxscaffold_7G00181850 Rroxscaffold_7G00196190 Rroxscaffold_7G00212980
rosa_rugosa Rorug02G0202700 Rorug06G0053300 Rorug06G0073400 Rorug06G0208900 Rorug07G0202300 Rorug07G0202300 Rorug07G0202300
rosa_samantha Rh1AG059200 Rh1AG066100 Rh1AG285800 Rh3AG222700 Rh3DG354600 Rh5BG508200 Rh5BG539300 Rh5BG541200 Rh5CG264600 Rh5CG531900 Rh5CG562800 Rh5CG563900 Rh5DG285300 Rh6AG094100 Rh6AG186000 Rh6AG186100 Rh6AG254700 Rh6BG189200 Rh6BG258000 Rh6CG186900 Rh6CG187000 Rh6DG178300 Rh6DG178700 Rh6DG349500 Rh7AG341800 Rh7AG341900 Rh7AG458600 Rh7BG332500 Rh7CG359600 Rh7DG340200 Rh7DG340300
rosa_wichuraiana Rw2G036370 Rw6G016030 Rw7G029050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 312
AciI CCGC 2 cut(s) 376, 382
AclWI GGATC 3 cut(s) 191, 689, 702
AcoI YGGCCR 1 cut(s) 813
AcuI CTGAAG 1 cut(s) 828
AcyI GRCGYC 1 cut(s) 486
AfiI CCNNNNNNNGG 3 cut(s) 132, 211, 588
AgsI TTSAA 6 cut(s) 8, 69, 391, 526, 596, 763
AluBI AGCT 8 cut(s) 247, 358, 367, 394, 415, 726, 737, 797
AluI AGCT 8 cut(s) 247, 358, 367, 394, 415, 726, 737, 797
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 561
AlwI GGATC 3 cut(s) 191, 689, 702
AoxI GGCC 2 cut(s) 607, 813
ApeKI GCWGC 3 cut(s) 412, 415, 797
AspS9I GGNCC 1 cut(s) 123
AsuC2I CCSGG 2 cut(s) 371, 433
AsuHPI GGTGA 2 cut(s) 215, 331
AvaII GGWCC 1 cut(s) 123
BalI TGGCCA 1 cut(s) 815
BamHI GGATCC 1 cut(s) 694
BanII GRGCYC 1 cut(s) 369
BauI CACGAG 3 cut(s) 170, 353, 559
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 402, 424, 784
BccI CCATC 2 cut(s) 652, 706
BceAI ACGGC 1 cut(s) 403
BclI TGATCA 2 cut(s) 256, 324
BcnI CCSGG 2 cut(s) 371, 433
BcoDI GTCTC 1 cut(s) 561
BfaI CTAG 2 cut(s) 344, 546
BfmI CTRYAG 1 cut(s) 511
BisI GCNGC 3 cut(s) 413, 416, 798
BlsI GCNGC 3 cut(s) 414, 417, 799
Bme1390I CCNGG 2 cut(s) 371, 433
Bme18I GGWCC 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 123
BmiI GGNNCC 2 cut(s) 125, 696
BmrFI CCNGG 2 cut(s) 371, 433
BmsI GCATC 2 cut(s) 341, 733
BpuEI CTTGAG 2 cut(s) 112, 560
BpuMI CCSGG 2 cut(s) 371, 433
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 432, 582
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 211, 588
Bse3DI GCAATG 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 432, 582
BseGI GGATG 3 cut(s) 61, 367, 477
BseLI CCNNNNNNNGG 3 cut(s) 132, 211, 588
BseMI GCAATG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 3 cut(s) 402, 424, 784
BshFI GGCC 2 cut(s) 609, 815
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 2 cut(s) 371, 432
BslFI GGGAC 1 cut(s) 675
BslI CCNNNNNNNGG 3 cut(s) 132, 211, 588
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 675
BsnI GGCC 2 cut(s) 609, 815
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 4 cut(s) 196, 256, 324, 694
BspACI CCGC 2 cut(s) 376, 382
BspANI GGCC 2 cut(s) 609, 815
BspLI GGNNCC 2 cut(s) 125, 696
BspPI GGATC 3 cut(s) 191, 689, 702
BspQI GCTCTTC 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 376
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 2 cut(s) 432, 582
BssMI GATC 4 cut(s) 196, 256, 324, 694
BssNI GRCGYC 1 cut(s) 486
BssSI CACGAG 3 cut(s) 170, 353, 559
BssT1I CCWWGG 1 cut(s) 582
Bst2BI CACGAG 3 cut(s) 170, 353, 559
Bst4CI ACNGT 2 cut(s) 18, 670
Bst6I CTCTTC 1 cut(s) 626
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 5 cut(s) 269, 273, 277, 396, 536
BstDEI CTNAG 1 cut(s) 772
BstF5I GGATG 3 cut(s) 61, 367, 477
BstKTI GATC 4 cut(s) 199, 259, 327, 697
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 4 cut(s) 196, 256, 324, 694
BstMWI GCNNNNNNNGC 7 cut(s) 281, 364, 373, 400, 409, 421, 743
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 2 cut(s) 369, 431
BstSFI CTRYAG 1 cut(s) 511
BstV1I GCAGC 3 cut(s) 402, 424, 784
BstX2I RGATCY 1 cut(s) 694
BstXI CCANNNNNNTGG 1 cut(s) 250
BstYI RGATCY 1 cut(s) 694
BsuRI GGCC 2 cut(s) 609, 815
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 3 cut(s) 61, 367, 477
Cac8I GCNNGC 5 cut(s) 269, 273, 277, 396, 536
Cfr13I GGNCC 1 cut(s) 123
CseI GACGC 2 cut(s) 290, 494
CviAII CATG 2 cut(s) 272, 749
DdeI CTNAG 1 cut(s) 772
DpnI GATC 4 cut(s) 198, 258, 326, 696
DpnII GATC 4 cut(s) 196, 256, 324, 694
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
EaeI YGGCCR 1 cut(s) 813
Eam1104I CTCTTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 626
EciI GGCGGA 1 cut(s) 397
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 582
Eco24I GRGCYC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 828
EcoICRI GAGCTC 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 582
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 2 cut(s) 275, 752
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 1 cut(s) 675
FatI CATG 2 cut(s) 271, 748
FbaI TGATCA 2 cut(s) 256, 324
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 3 cut(s) 413, 416, 798
FokI GGATG 3 cut(s) 68, 374, 484
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 3 cut(s) 413, 416, 798
FspBI CTAG 2 cut(s) 344, 546
GluI GCNGC 3 cut(s) 413, 416, 798
HaeIII GGCC 2 cut(s) 609, 815
HapII CCGG 2 cut(s) 371, 432
HgaI GACGC 2 cut(s) 290, 494
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 2 cut(s) 275, 752
HincII GTYRAC 2 cut(s) 508, 601
HindII GTYRAC 2 cut(s) 508, 601
HindIII AAGCTT 3 cut(s) 245, 724, 735
HinfI GANTC 5 cut(s) 175, 442, 448, 475, 554
HpaI GTTAAC 1 cut(s) 508
HpaII CCGG 2 cut(s) 371, 432
HphI GGTGA 2 cut(s) 215, 331
Hpy166II GTNNAC 4 cut(s) 313, 352, 508, 601
Hpy188I TCNGA 2 cut(s) 62, 447
Hpy188III TCNNGA 6 cut(s) 140, 172, 344, 353, 479, 625
Hpy8I GTNNAC 4 cut(s) 313, 352, 508, 601
Hpy99I CGWCG 3 cut(s) 18, 284, 424
HpyAV CCTTC 3 cut(s) 510, 588, 620
HpyCH4III ACNGT 2 cut(s) 18, 670
HpyCH4V TGCA 6 cut(s) 267, 294, 648, 731, 746, 784
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 364, 373, 400, 409, 421, 743
HpyF3I CTNAG 1 cut(s) 772
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 2 cut(s) 275, 752
Ksp22I TGATCA 2 cut(s) 256, 324
KspAI GTTAAC 1 cut(s) 508
Kzo9I GATC 4 cut(s) 196, 256, 324, 694
LguI GCTCTTC 1 cut(s) 626
LmnI GCTCC 2 cut(s) 372, 373
Lsp1109I GCAGC 3 cut(s) 402, 424, 784
LweI GCATC 2 cut(s) 341, 733
MaeI CTAG 2 cut(s) 344, 546
MaeIII GTNAC 1 cut(s) 221
MalI GATC 4 cut(s) 198, 258, 326, 696
MbiI CCGCTC 1 cut(s) 376
MboI GATC 4 cut(s) 196, 256, 324, 694
MboII GAAGA 5 cut(s) 475, 514, 613, 640, 764
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 1 cut(s) 369
MlsI TGGCCA 1 cut(s) 815
MluCI AATT 2 cut(s) 206, 427
MluNI TGGCCA 1 cut(s) 815
MlyI GAGTC 2 cut(s) 484, 548
MnlI CCTC 8 cut(s) 40, 87, 179, 340, 378, 475, 535, 557
Mox20I TGGCCA 1 cut(s) 815
MscI TGGCCA 1 cut(s) 815
MseI TTAA 5 cut(s) 20, 75, 158, 209, 507
MslI CAYNNNNRTG 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 815
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 2 cut(s) 371, 432
MspR9I CCNGG 2 cut(s) 371, 433
MwoI GCNNNNNNNGC 7 cut(s) 281, 364, 373, 400, 409, 421, 743
NciI CCSGG 2 cut(s) 371, 433
NdeII GATC 4 cut(s) 196, 256, 324, 694
NlaIII CATG 2 cut(s) 275, 752
NlaIV GGNNCC 2 cut(s) 125, 696
NmeAIII GCCGAG 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 221
NspI RCATGY 1 cut(s) 275
PaeI GCATGC 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 626
PfeI GAWTC 3 cut(s) 175, 442, 448
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 3 cut(s) 414, 417, 799
PleI GAGTC 2 cut(s) 483, 548
PpsI GAGTC 2 cut(s) 483, 548
PpuMI RGGWCCY 1 cut(s) 123
Psp124BI GAGCTC 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 123
PspN4I GGNNCC 2 cut(s) 125, 696
PspPI GGNCC 1 cut(s) 123
PspPPI RGGWCCY 1 cut(s) 123
PsuI RGATCY 1 cut(s) 694
PvuII CAGCTG 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 1 cut(s) 369
SapI GCTCTTC 1 cut(s) 626
SaqAI TTAA 5 cut(s) 20, 75, 158, 209, 507
SatI GCNGC 3 cut(s) 413, 416, 798
Sau3AI GATC 4 cut(s) 196, 256, 324, 694
Sau96I GGNCC 1 cut(s) 123
SchI GAGTC 2 cut(s) 484, 548
ScrFI CCNGG 2 cut(s) 371, 433
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 2 cut(s) 341, 733
SfcI CTRYAG 1 cut(s) 511
SinI GGWCC 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 2 cut(s) 91, 539
SmoI CTYRAG 2 cut(s) 91, 539
SphI GCATGC 1 cut(s) 275
Sse9I AATT 2 cut(s) 206, 427
SsiI CCGC 2 cut(s) 376, 382
SspMI CTAG 2 cut(s) 344, 546
SstI GAGCTC 1 cut(s) 369
StyD4I CCNGG 2 cut(s) 369, 431
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 2 cut(s) 18, 670
TaqI TCGA 2 cut(s) 13, 45
TasI AATT 2 cut(s) 206, 427
TfiI GAWTC 3 cut(s) 175, 442, 448
Tru1I TTAA 5 cut(s) 20, 75, 158, 209, 507
Tru9I TTAA 5 cut(s) 20, 75, 158, 209, 507
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 3 cut(s) 412, 415, 797
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 246, 765
TspGWI ACGGA 1 cut(s) 724
VpaK11BI GGWCC 1 cut(s) 123
XbaI TCTAGA 1 cut(s) 343
XceI RCATGY 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 2 cut(s) 344, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.