AT5G62180

AtCXE20 AtCXE20 (Arabidopsis thaliana carboxyesterase 20)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000860
Physical Location & Seq
Reverse (-)
24978718 .. 24980075
1358 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62180.1

Sequence Viewer

Length: 984 bp
ATGTCCGAACCAAGTCCAATCGCTGATCCTTATGCTTATCTTAACATCGTAAATAATCCAGATGGTTCAATCACTCGCGACCTCAGCAACTTCCCCTGCACCGCAGCTACACCTGATCCTTCCCCACTAAACCCCGCCGTTTCAAAAGATCTTCCCGTTAACCAATTAAAGTCAACGTGGCTCCGTCTCTACCTTCCCTCCTCCGCCGTCAACGAGGGTAACGTTTCGTCTCAGAAACTCCCCATTGTTGTGTATTACCATGGCGGAGGATTCATCCTCTGCAGCGTCGATATGCAACTCTTCCACGATTTCTGCTCCGAGGTGGCTCGTGATCTCAACGCCATCGTCGTATCTCCTTCGTACCGATTAGCTCCAGAGCATCGATTACCGGCGGCGTATGACGACGGAGTTGAGGCGTTGGATTGGATTAAAACCTCCGACGATGAGTGGATCAAATCTCACGCCGATTTCTCAAATGTGTTTCTTATGGGAACTAGCGCCGGAGGGAATTTGGCTTACAACGTCGGACTAAGATCCGTCGATTCCGTCTCCGATCTGAGTCCGTTACAGATCCGTGGATTGATTCTACACCATCCATTCTTCGGCGGAGAAGAGCGAAGTGAATCTGAGATTAGACTTATGAACGATCAGGTTTGTCCTCCCATAGTCACCGATGTAATGTGGGATCTGTCTCTTCCCGTCGGCGTTGACCGAGATCATGAGTATTCGAATCCGACGGTTGGAGATGGATCGGAGAAGTTGGAAAAAATCGGACGGTTGAGATGGAAAGTGATGATGATTGGAGGAGAGGATGATCCGATGATAGATCTGCAGAAAGATGTGGCAAAGCTGATGAAGAAGAAAGGAGTAGAAGTGGTGGAGCATTATACCGGCGGCCATGTTCACGGTGCTGAAATCAGAGATCCTTCTAAACGTAAAACTCTGTTTCTTTCCATCAAAAACTTCATATTCTCTGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

327

Amino Acids

36.18

Weight (kDa)

5.11

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 64 - 172 4e-06 Carboxylesterase family
BD-FAE PF20434 76 - 179 5.9e-13 BD-FAE
Say1_Mug180 PF10340 81 - 178 5.6e-06 Steryl acetyl hydrolase
Abhydrolase_3 PF07859 83 - 304 2.7e-55 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 78
AciI CCGC 7 cut(s) 102, 135, 204, 264, 392, 606, 894
AclI AACGTT 1 cut(s) 222
AclWI GGATC 9 cut(s) 20, 110, 458, 528, 565, 693, 757, 809, 917
AcoI YGGCCR 1 cut(s) 895
AcsI RAATTY 1 cut(s) 508
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 2 cut(s) 602, 740
AgsI TTSAA 2 cut(s) 69, 144
AluBI AGCT 3 cut(s) 107, 371, 850
AluI AGCT 3 cut(s) 107, 371, 850
Alw26I GTCTC 4 cut(s) 191, 234, 553, 696
AlwI GGATC 9 cut(s) 20, 110, 458, 528, 565, 693, 757, 809, 917
AoxI GGCC 1 cut(s) 895
ApeKI GCWGC 2 cut(s) 104, 282
ApoI RAATTY 1 cut(s) 508
AspLEI GCGC 1 cut(s) 500
AsuHPI GGTGA 1 cut(s) 661
AsuII TTCGAA 1 cut(s) 728
BauI CACGAG 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 116, 294
BccI CCATC 6 cut(s) 56, 350, 600, 740, 777, 962
BceAI ACGGC 2 cut(s) 122, 191
BcoDI GTCTC 4 cut(s) 191, 234, 553, 696
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 2 cut(s) 280, 830
BfoI RGCGCY 1 cut(s) 501
BglII AGATCT 2 cut(s) 148, 826
BisI GCNGC 4 cut(s) 105, 283, 393, 895
BlsI GCNGC 4 cut(s) 106, 284, 394, 896
BmiI GGNNCC 1 cut(s) 182
BmsI GCATC 1 cut(s) 388
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 728
Bsa29I ATCGAT 1 cut(s) 382
BsaJI CCNNGG 3 cut(s) 259, 318, 574
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 858, 888
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 740
Bse118I RCCGGY 2 cut(s) 388, 890
BseCI ATCGAT 1 cut(s) 382
BseDI CCNNGG 3 cut(s) 259, 318, 574
BseGI GGATG 3 cut(s) 273, 592, 817
BseLI CCNNNNNNNGG 2 cut(s) 602, 740
BseMII CTCAG 4 cut(s) 97, 245, 548, 618
BseRI GAGGAG 2 cut(s) 190, 819
BseXI GCAGC 2 cut(s) 116, 294
BsgI GTGCAG 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 897
BshVI ATCGAT 1 cut(s) 382
BsiSI CCGG 3 cut(s) 389, 501, 891
BslI CCNNNNNNNGG 2 cut(s) 602, 740
BsmAI GTCTC 4 cut(s) 191, 234, 553, 696
BsmBI CGTCTC 3 cut(s) 191, 234, 553
BsnI GGCC 1 cut(s) 897
Bsp119I TTCGAA 1 cut(s) 728
Bsp19I CCATGG 1 cut(s) 259
Bsp68I TCGCGA 1 cut(s) 78
BspACI CCGC 7 cut(s) 102, 135, 204, 264, 392, 606, 894
BspANI GGCC 1 cut(s) 897
BspCNI CTCAG 4 cut(s) 96, 244, 549, 619
BspDI ATCGAT 1 cut(s) 382
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 1 cut(s) 182
BspMAI CTGCAG 2 cut(s) 284, 834
BspPI GGATC 9 cut(s) 20, 110, 458, 528, 565, 693, 757, 809, 917
BspQI GCTCTTC 1 cut(s) 606
BspT104I TTCGAA 1 cut(s) 728
BsrFI RCCGGY 2 cut(s) 388, 890
BssAI RCCGGY 2 cut(s) 388, 890
BssECI CCNNGG 3 cut(s) 259, 318, 574
BssSI CACGAG 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 259
Bst2BI CACGAG 1 cut(s) 327
Bst4CI ACNGT 3 cut(s) 739, 777, 908
Bst6I CTCTTC 3 cut(s) 305, 606, 699
BstBI TTCGAA 1 cut(s) 728
BstDEI CTNAG 5 cut(s) 83, 231, 530, 557, 627
BstDSI CCRYGG 2 cut(s) 259, 574
BstF5I GGATG 3 cut(s) 273, 592, 817
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 1 cut(s) 500
BstMAI GTCTC 4 cut(s) 191, 234, 553, 696
BstMWI GCNNNNNNNGC 1 cut(s) 84
BstSFI CTRYAG 2 cut(s) 280, 830
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 2 cut(s) 116, 294
BstX2I RGATCY 6 cut(s) 148, 533, 570, 685, 826, 922
BstYI RGATCY 6 cut(s) 148, 533, 570, 685, 826, 922
Bsu15I ATCGAT 1 cut(s) 382
BsuRI GGCC 1 cut(s) 897
BsuTUI ATCGAT 1 cut(s) 382
BtgI CCRYGG 2 cut(s) 259, 574
BtsCI GGATG 3 cut(s) 273, 592, 817
BtuMI TCGCGA 1 cut(s) 78
CciI TCATGA 1 cut(s) 718
CfoI GCGC 1 cut(s) 500
Cfr10I RCCGGY 2 cut(s) 388, 890
ClaI ATCGAT 1 cut(s) 382
CseI GACGC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 361
CviAII CATG 3 cut(s) 260, 719, 899
CviJI RGCY 7 cut(s) 107, 181, 326, 371, 515, 850, 897
CviKI_1 RGCY 7 cut(s) 107, 181, 326, 371, 515, 850, 897
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 5 cut(s) 83, 231, 530, 557, 627
EaeI YGGCCR 1 cut(s) 895
Eam1104I CTCTTC 3 cut(s) 305, 606, 699
EarI CTCTTC 3 cut(s) 305, 606, 699
EciI GGCGGA 3 cut(s) 193, 279, 621
Eco130I CCWWGG 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
Esp3I CGTCTC 3 cut(s) 191, 234, 553
FaeI CATG 3 cut(s) 263, 722, 902
FatI CATG 3 cut(s) 259, 718, 898
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 4 cut(s) 105, 283, 393, 895
FokI GGATG 3 cut(s) 260, 579, 824
Fsp4HI GCNGC 4 cut(s) 105, 283, 393, 895
FspBI CTAG 1 cut(s) 495
GlaI GCGC 1 cut(s) 499
GluI GCNGC 4 cut(s) 105, 283, 393, 895
GsuI CTGGAG 1 cut(s) 357
HaeII RGCGCY 1 cut(s) 501
HaeIII GGCC 1 cut(s) 897
HapII CCGG 3 cut(s) 389, 501, 891
HgaI GACGC 1 cut(s) 274
HhaI GCGC 1 cut(s) 500
Hin1II CATG 3 cut(s) 263, 722, 902
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HincII GTYRAC 4 cut(s) 160, 174, 211, 709
HindII GTYRAC 4 cut(s) 160, 174, 211, 709
HinfI GANTC 6 cut(s) 270, 542, 559, 583, 623, 730
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 3 cut(s) 389, 501, 891
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 5 cut(s) 160, 174, 211, 709, 904
Hpy188III TCNNGA 5 cut(s) 59, 77, 329, 374, 719
Hpy8I GTNNAC 5 cut(s) 160, 174, 211, 709, 904
Hpy99I CGWCG 8 cut(s) 290, 350, 407, 443, 527, 542, 704, 739
HpyAV CCTTC 4 cut(s) 129, 203, 366, 936
HpyCH4III ACNGT 3 cut(s) 739, 777, 908
HpyCH4IV ACGT 4 cut(s) 176, 222, 522, 934
HpyCH4V TGCA 4 cut(s) 99, 282, 295, 832
HpyF10VI GCNNNNNNNGC 1 cut(s) 84
HpyF3I CTNAG 5 cut(s) 83, 231, 530, 557, 627
HpySE526I ACGT 4 cut(s) 176, 222, 522, 934
Hsp92II CATG 3 cut(s) 263, 722, 902
HspAI GCGC 1 cut(s) 498
KspAI GTTAAC 1 cut(s) 160
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 4 cut(s) 186, 320, 376, 880
LpnPI CCDG 8 cut(s) 72, 109, 126, 387, 402, 514, 635, 904
Lsp1109I GCAGC 2 cut(s) 116, 294
LweI GCATC 1 cut(s) 388
MaeI CTAG 1 cut(s) 495
MaeII ACGT 4 cut(s) 176, 222, 522, 934
MaeIII GTNAC 3 cut(s) 218, 564, 667
MboII GAAGA 7 cut(s) 143, 292, 592, 623, 686, 868, 871
MflI RGATCY 6 cut(s) 148, 533, 570, 685, 826, 922
MluCI AATT 2 cut(s) 164, 508
MlyI GAGTC 1 cut(s) 568
MmeI TCCRAC 6 cut(s) 399, 462, 505, 721, 741, 758
MseI TTAA 4 cut(s) 42, 159, 167, 429
MslI CAYNNNNRTG 1 cut(s) 248
MspI CCGG 3 cut(s) 389, 501, 891
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 84
NcoI CCATGG 1 cut(s) 259
NlaIII CATG 3 cut(s) 263, 722, 902
NlaIV GGNNCC 1 cut(s) 182
NmuCI GTSAC 1 cut(s) 667
NruI TCGCGA 1 cut(s) 78
NspV TTCGAA 1 cut(s) 728
PagI TCATGA 1 cut(s) 718
PciSI GCTCTTC 1 cut(s) 606
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 345
PfeI GAWTC 5 cut(s) 270, 542, 583, 623, 730
PkrI GCNGC 4 cut(s) 106, 284, 394, 896
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
Psp1406I AACGTT 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 182
PstI CTGCAG 2 cut(s) 284, 834
PsuI RGATCY 6 cut(s) 148, 533, 570, 685, 826, 922
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 4 cut(s) 42, 159, 167, 429
SatI GCNGC 4 cut(s) 105, 283, 393, 895
SchI GAGTC 1 cut(s) 568
SfaNI GCATC 1 cut(s) 388
SfcI CTRYAG 2 cut(s) 280, 830
SfuI TTCGAA 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 2 cut(s) 164, 508
SsiI CCGC 7 cut(s) 102, 135, 204, 264, 392, 606, 894
SspMI CTAG 1 cut(s) 495
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 3 cut(s) 739, 777, 908
TaiI ACGT 4 cut(s) 179, 225, 525, 937
TaqI TCGA 4 cut(s) 288, 382, 540, 728
TaqII GACCGA 1 cut(s) 726
TasI AATT 2 cut(s) 164, 508
TauI GCSGC 2 cut(s) 395, 897
TfiI GAWTC 5 cut(s) 270, 542, 583, 623, 730
Tru1I TTAA 4 cut(s) 42, 159, 167, 429
Tru9I TTAA 4 cut(s) 42, 159, 167, 429
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 2 cut(s) 104, 282
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 4 cut(s) 262, 656, 869, 955
TspGWI ACGGA 6 cut(s) 173, 420, 526, 535, 552, 563
XapI RAATTY 1 cut(s) 508
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.