pycom06g04390

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000860
Physical Location & Seq
Reverse (-)
5656255 .. 5657180
926 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g04390.1

Sequence Viewer

Length: 885 bp
ATGCCAGAACAAACGGCAGCTTCCACTATGTCCAATCCCACCGTCGATAATTCCGATCCCTACAAGTTCCTTAAACTTGCATCCAATCCGGACGGCACAATCACACGCTTGACCAAATTTCCCAGCAGCCCAGCCACTTGCGATCCCAATCTTCCCACCCCAATTTTCACCAAAGACGTCCCCATCAACCAATCAAACAACACCTGGGTCCGAATATTCCTGCCCCGCCACCTGCTCGACCACTCCTCTGCAACCCCGCCCGCCAAGCTACCGCTCGTGGTGTACTTCCACGGCGGCGGGTTCATACAGCTCAGCGCAGCCTCAACCATAATGGACAACTTCTACGTAAACTTGGCTACCCAAGTCCCCGTGATCATCGCATCGGTGGAGTACAGGCTGGCTCCGGAGCACCGGCTGCCCGCTGCGTACGAGGACGCGGTGGAAGCGCTGCACTTTATCGAAACAGCCCCAGACCACTGGCTAAGGGACTATGCTGACTTGTGGAATTGTTACCTCATGGGCAGCAGCGCCGGTGGCAACATAGCCTACCATGCGGGACTTCGTGCGGCTGCGGAAGCTGACGATTTTCACCGGTTGAAGATCCGAGGGTTGATATTGCATCAACCGTTCTTCAATGGGGCCCAGAGAAGCAGATCTGAGTCAAGGTTGGAGAACAACCCCGCGTTGCCGCTGAGTAGCAGCGATCTGATGTGGGAACTGTCGTTGCCCGTGGGCGTTGACCGTGACCATGAGTATAGCAATCCAATGGTGGGGGATGGTTTGAGTGAGAGGTTGGATCAATTGAAGGCGGCTGGATTCAGGGTTTTGGGAAGTGGCGAAGATGTTGAAAGAAAATGGGGTCGAAGTGGTGGAGCATTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

295

Amino Acids

32.41

Weight (kDa)

5.66

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 88 - 191 3e-13 BD-FAE
Abhydrolase_3 PF07859 93 - 286 1.6e-40 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 240
AatII GACGTC 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 240
AccBSI CCGCTC 1 cut(s) 274
AccII CGCG 2 cut(s) 437, 683
AccIII TCCGGA 2 cut(s) 88, 403
AclWI GGATC 4 cut(s) 50, 137, 595, 804
AcsI RAATTY 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 3 cut(s) 284, 392, 428
AfeI AGCGCT 1 cut(s) 447
AfiI CCNNNNNNNGG 1 cut(s) 770
AgeI ACCGGT 1 cut(s) 591
AgsI TTSAA 4 cut(s) 598, 634, 805, 848
AjnI CCWGG 1 cut(s) 203
AluBI AGCT 4 cut(s) 20, 268, 310, 578
AluI AGCT 4 cut(s) 20, 268, 310, 578
Alw21I GWGCWC 1 cut(s) 411
AlwI GGATC 4 cut(s) 50, 137, 595, 804
Aor13HI TCCGGA 2 cut(s) 88, 403
Aor51HI AGCGCT 1 cut(s) 447
AoxI GGCC 1 cut(s) 639
ApaI GGGCCC 1 cut(s) 643
ApoI RAATTY 1 cut(s) 116
AsiGI ACCGGT 1 cut(s) 591
AspLEI GCGC 3 cut(s) 317, 448, 530
AspS9I GGNCC 3 cut(s) 208, 639, 640
AsuHPI GGTGA 2 cut(s) 160, 581
AvaII GGWCC 1 cut(s) 208
BaeGI GKGCMC 1 cut(s) 643
BanII GRGCYC 1 cut(s) 643
BauI CACGAG 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 411
BccI CCATC 2 cut(s) 191, 770
BceAI ACGGC 3 cut(s) 30, 109, 307
BciT130I CCWGG 1 cut(s) 205
BclI TGATCA 1 cut(s) 372
BfoI RGCGCY 2 cut(s) 449, 531
BfuAI ACCTGC 1 cut(s) 240
BglII AGATCT 1 cut(s) 653
BlpI GCTNAGC 1 cut(s) 311
Bme1390I CCNGG 1 cut(s) 205
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 3 cut(s) 208, 639, 640
BmiI GGNNCC 4 cut(s) 209, 402, 640, 641
BmrFI CCNGG 1 cut(s) 205
BmsI GCATC 3 cut(s) 89, 389, 628
Bpu10I CCTNAGC 1 cut(s) 482
Bpu1102I GCTNAGC 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 204, 289, 604, 729
BsaWI WCCGGW 3 cut(s) 88, 403, 591
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse118I RCCGGY 3 cut(s) 411, 530, 591
Bse1I ACTGG 1 cut(s) 482
Bse8I GATNNNNATC 1 cut(s) 147
BseAI TCCGGA 2 cut(s) 88, 403
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 4 cut(s) 204, 289, 604, 729
BseGI GGATG 2 cut(s) 80, 781
BseJI GATNNNNATC 1 cut(s) 147
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMII CTCAG 3 cut(s) 325, 648, 683
BseNI ACTGG 1 cut(s) 482
BseRI GAGGAG 1 cut(s) 235
BseSI GKGCMC 1 cut(s) 643
BseYI CCCAGC 2 cut(s) 122, 130
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 2 cut(s) 437, 683
BshFI GGCC 1 cut(s) 641
BshTI ACCGGT 1 cut(s) 591
BsiHKAI GWGCWC 1 cut(s) 411
BsiSI CCGG 5 cut(s) 89, 404, 412, 531, 592
BsiWI CGTACG 1 cut(s) 426
BslFI GGGAC 4 cut(s) 164, 350, 500, 570
BslI CCNNNNNNNGG 1 cut(s) 770
BsmFI GGGAC 4 cut(s) 164, 350, 500, 570
BsnI GGCC 1 cut(s) 641
Bsp120I GGGCCC 1 cut(s) 639
Bsp1286I GDGCHC 2 cut(s) 411, 643
Bsp13I TCCGGA 2 cut(s) 88, 403
Bsp143I GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
Bsp1720I GCTNAGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 3 cut(s) 324, 649, 684
BspEI TCCGGA 2 cut(s) 88, 403
BspFNI CGCG 2 cut(s) 437, 683
BspLI GGNNCC 4 cut(s) 209, 402, 640, 641
BspMI ACCTGC 1 cut(s) 240
BspPI GGATC 4 cut(s) 50, 137, 595, 804
BsrBI CCGCTC 1 cut(s) 274
BsrFI RCCGGY 3 cut(s) 411, 530, 591
BsrI ACTGG 1 cut(s) 482
BssAI RCCGGY 3 cut(s) 411, 530, 591
BssECI CCNNGG 4 cut(s) 204, 289, 604, 729
BssMI GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 275
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 1 cut(s) 205
Bst4CI ACNGT 4 cut(s) 43, 627, 720, 743
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 415
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 261, 399, 420
BstDEI CTNAG 4 cut(s) 311, 482, 657, 692
BstDSI CCRYGG 2 cut(s) 289, 729
BstF5I GGATG 2 cut(s) 80, 781
BstFNI CGCG 2 cut(s) 437, 683
BstH2I RGCGCY 2 cut(s) 449, 531
BstHHI GCGC 3 cut(s) 317, 448, 530
BstKTI GATC 7 cut(s) 58, 145, 375, 603, 656, 706, 799
BstMBI GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
BstMWI GCNNNNNNNGC 6 cut(s) 265, 415, 443, 534, 551, 575
BstNI CCWGG 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 203
BstSLI GKGCMC 1 cut(s) 643
BstSNI TACGTA 1 cut(s) 346
BstUI CGCG 2 cut(s) 437, 683
BstX2I RGATCY 2 cut(s) 600, 653
BstXI CCANNNNNNTGG 1 cut(s) 477
BstYI RGATCY 2 cut(s) 600, 653
BsuRI GGCC 1 cut(s) 641
BtgI CCRYGG 2 cut(s) 289, 729
BtgZI GCGATG 1 cut(s) 361
BtsCI GGATG 2 cut(s) 80, 781
BtsIMutI CAGTG 1 cut(s) 475
BveI ACCTGC 1 cut(s) 240
Cac8I GCNNGC 3 cut(s) 261, 399, 420
CfoI GCGC 3 cut(s) 317, 448, 530
Cfr10I RCCGGY 3 cut(s) 411, 530, 591
Cfr13I GGNCC 3 cut(s) 208, 639, 640
CseI GACGC 1 cut(s) 443
Csp6I GTAC 3 cut(s) 283, 391, 427
CspAI ACCGGT 1 cut(s) 591
CviAII CATG 3 cut(s) 517, 551, 749
CviQI GTAC 3 cut(s) 283, 391, 427
DdeI CTNAG 4 cut(s) 311, 482, 657, 692
DpnI GATC 7 cut(s) 57, 144, 374, 602, 655, 705, 798
DpnII GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
Eco105I TACGTA 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 208
Eco47III AGCGCT 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 203
EcoT38I GRGCYC 1 cut(s) 643
FaeI CATG 3 cut(s) 520, 554, 752
FaiI YATR 9 cut(s) 29, 305, 329, 492, 518, 542, 552, 750, 756
FaqI GGGAC 4 cut(s) 164, 350, 500, 570
FatI CATG 3 cut(s) 516, 550, 748
FauI CCCGC 7 cut(s) 233, 264, 268, 290, 427, 547, 688
FbaI TGATCA 1 cut(s) 372
FokI GGATG 2 cut(s) 67, 788
FriOI GRGCYC 1 cut(s) 643
GlaI GCGC 3 cut(s) 316, 447, 529
GsaI CCCAGC 2 cut(s) 126, 134
HaeII RGCGCY 2 cut(s) 449, 531
HaeIII GGCC 1 cut(s) 641
HapII CCGG 5 cut(s) 89, 404, 412, 531, 592
HgaI GACGC 1 cut(s) 443
HhaI GCGC 3 cut(s) 317, 448, 530
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 520, 554, 752
Hin6I GCGC 3 cut(s) 315, 446, 528
HinP1I GCGC 3 cut(s) 315, 446, 528
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 2 cut(s) 659, 816
HpaII CCGG 5 cut(s) 89, 404, 412, 531, 592
HphI GGTGA 2 cut(s) 160, 581
Hpy166II GTNNAC 3 cut(s) 283, 349, 739
Hpy188I TCNGA 5 cut(s) 55, 212, 605, 658, 708
Hpy188III TCNNGA 2 cut(s) 89, 404
Hpy8I GTNNAC 3 cut(s) 283, 349, 739
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 799
HpyCH4III ACNGT 4 cut(s) 43, 627, 720, 743
HpyCH4IV ACGT 2 cut(s) 177, 345
HpyCH4V TGCA 4 cut(s) 80, 251, 451, 619
HpyF10VI GCNNNNNNNGC 6 cut(s) 265, 415, 443, 534, 551, 575
HpyF3I CTNAG 4 cut(s) 311, 482, 657, 692
HpySE526I ACGT 2 cut(s) 177, 345
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 520, 554, 752
HspAI GCGC 3 cut(s) 315, 446, 528
Kpn2I TCCGGA 2 cut(s) 88, 403
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
LmnI GCTCC 3 cut(s) 406, 406, 872
LweI GCATC 3 cut(s) 89, 389, 628
MaeII ACGT 2 cut(s) 177, 345
MaeIII GTNAC 2 cut(s) 509, 743
MalI GATC 7 cut(s) 57, 144, 374, 602, 655, 705, 798
MbiI CCGCTC 1 cut(s) 274
MboI GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
MboII GAAGA 4 cut(s) 143, 610, 622, 851
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 2 cut(s) 600, 653
MhlI GDGCHC 2 cut(s) 411, 643
MluCI AATT 5 cut(s) 49, 116, 162, 505, 800
MlyI GAGTC 1 cut(s) 668
MmeI TCCRAC 2 cut(s) 648, 774
MnlI CCTC 6 cut(s) 256, 331, 424, 524, 599, 783
MroI TCCGGA 2 cut(s) 88, 403
MseI TTAA 1 cut(s) 72
MspA1I CMGCKG 2 cut(s) 422, 691
MspI CCGG 5 cut(s) 89, 404, 412, 531, 592
MspR9I CCNGG 1 cut(s) 205
MunI CAATTG 1 cut(s) 800
MvaI CCWGG 1 cut(s) 205
MvnI CGCG 2 cut(s) 437, 683
MwoI GCNNNNNNNGC 6 cut(s) 265, 415, 443, 534, 551, 575
NdeII GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
NlaIII CATG 3 cut(s) 520, 554, 752
NlaIV GGNNCC 4 cut(s) 209, 402, 640, 641
NmuCI GTSAC 1 cut(s) 743
PaqCI CACCTGC 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 1 cut(s) 816
Pfl23II CGTACG 1 cut(s) 426
PinAI ACCGGT 1 cut(s) 591
PleI GAGTC 1 cut(s) 667
PpsI GAGTC 1 cut(s) 667
Ppu21I YACGTR 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 203
PspFI CCCAGC 2 cut(s) 122, 130
PspGI CCWGG 1 cut(s) 203
PspLI CGTACG 1 cut(s) 426
PspN4I GGNNCC 4 cut(s) 209, 402, 640, 641
PspOMI GGGCCC 1 cut(s) 639
PspPI GGNCC 3 cut(s) 208, 639, 640
PsuI RGATCY 2 cut(s) 600, 653
RsaI GTAC 3 cut(s) 284, 392, 428
RsaNI GTAC 3 cut(s) 283, 391, 427
SaqAI TTAA 1 cut(s) 72
Sau3AI GATC 7 cut(s) 55, 142, 372, 600, 653, 703, 796
Sau96I GGNCC 3 cut(s) 208, 639, 640
SchI GAGTC 1 cut(s) 668
ScrFI CCNGG 1 cut(s) 205
SduI GDGCHC 2 cut(s) 411, 643
SfaNI GCATC 3 cut(s) 89, 389, 628
SgrAI CRCCGGYG 1 cut(s) 530
SinI GGWCC 1 cut(s) 208
SnaBI TACGTA 1 cut(s) 346
Sse9I AATT 5 cut(s) 49, 116, 162, 505, 800
SspI AATATT 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 203
TaaI ACNGT 4 cut(s) 43, 627, 720, 743
TaiI ACGT 2 cut(s) 180, 348
TaqI TCGA 4 cut(s) 45, 237, 459, 862
TasI AATT 5 cut(s) 49, 116, 162, 505, 800
TatI WGTACW 2 cut(s) 282, 390
TauI GCSGC 4 cut(s) 297, 569, 691, 812
TfiI GAWTC 1 cut(s) 816
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 1 cut(s) 482
TseFI GTSAC 1 cut(s) 743
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 1 cut(s) 292
TspRI CASTG 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 208
XapI RAATTY 1 cut(s) 116
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.