MD06G1052300.v1.1

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000860
Physical Location & Seq
Reverse (-)
7542395 .. 7543510
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1052300.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGATATTTAATTCGACCACCAACTTTCTTTTAATAGACTCCATCTTGAGCATTTGGTTTCAATCATTCACACATACACTCACTCTCTCGCACATCTCCAATATTTCCCAAAGTCAGGAAATGCCAGAACAAACGGCAGCTTCCACTATGTCCAATCCCACAGTCGATAATCCCGATCCCTACAAGTTCCTCAACCTTGCATCCAATCCGGACGGAACAATCACACGCTTGACCAAATTTCCCGACAGCCCAGCCACCTGCGATCCCAACCTTCCCACCCCATTTTTCACCAAAGACGTCCCCATCAACCAATCAAACAACACCTGGGTCCGAATATTCCTGCCCCGCCACCTGCTCGACCACTCCTCCGCAACCCCGCCCGCCAAGCTACCGCTCGTGGTGTACTTCCACGGCGGCGGGTTCATACTGCTTAGCGCAGCCTCAACCATAATGGACGACTTCTGCGTCAACTTGGCTACCCAAGTCCCCGTGATCATCGCATCGGTGGAGTACAGGCTGGCTCCGGAGCACCGGCTGCCCGCTGCTTACGAGGACGCGGTGGAAGCGCTGCACTTTATCAAAACAGCCCCAGACAACTGGCTAAGGGACTATGCTGACTTGTCGAATTGTTACCTCATGGGCAGCAGCGCCGGTGGCAACATAGCCTACCATGCGGGACTTCGTGCGGCTGCGGAAGCTGACGATTTTCACCGGTTGAAGATCCGAGGGTTGATATTGCATCAACCGTTCTTCAGTGGGGCCCAGAGAAGCAGATCTGAGTCAAGGTTGGAGAACAACCCCACGTTGCCGCTGAGTAGTAGCGATCTGATGTGGGAACTGTCGTTGCCCATGGGCGTTGACCGCGACCATGAGTATTGCAATCCAATGGTGGGGGATGGTTTGAGTGAGAAGTTGGATCAATTGAAGGCAGCTGGATTCAGGGTTTTGGTGGCTGGATCCGATGGAGATTTACTGATTGATCGTCAGATGGAGGTGGCGAAGATGTTGAAGGAAAATGGGGTCAAAGTGGTGGAACATTTTGGTGAAGGAGGTTATCATGGGGTTATTGACAAGGATCTGTTTGCGGTTGTAAAAAGTTTCACATCTTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

372

Amino Acids

40.9

Weight (kDa)

5.31

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 128 - 230 2.5e-13 BD-FAE
Abhydrolase_3 PF07859 133 - 355 1.4e-51 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 266, 360
AasI GACNNNNNNGTC 1 cut(s) 464
AatII GACGTC 1 cut(s) 300
Acc36I ACCTGC 2 cut(s) 266, 360
AccBSI CCGCTC 1 cut(s) 394
AccII CGCG 2 cut(s) 557, 864
AccIII TCCGGA 2 cut(s) 208, 523
AclWI GGATC 7 cut(s) 170, 257, 715, 924, 951, 964, 1083
AcsI RAATTY 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 736
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 2 cut(s) 404, 512
AfeI AGCGCT 1 cut(s) 567
AfiI CCNNNNNNNGG 2 cut(s) 115, 890
AgeI ACCGGT 1 cut(s) 711
AgsI TTSAA 4 cut(s) 62, 718, 925, 1009
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 4 cut(s) 140, 388, 698, 932
AluI AGCT 4 cut(s) 140, 388, 698, 932
Alw21I GWGCWC 1 cut(s) 531
AlwI GGATC 7 cut(s) 170, 257, 715, 924, 951, 964, 1083
Aor13HI TCCGGA 2 cut(s) 208, 523
Aor51HI AGCGCT 1 cut(s) 567
AoxI GGCC 1 cut(s) 759
ApaI GGGCCC 1 cut(s) 763
ApeKI GCWGC 9 cut(s) 137, 437, 535, 542, 568, 642, 645, 689, 929
ApoI RAATTY 1 cut(s) 236
AsiGI ACCGGT 1 cut(s) 711
AspLEI GCGC 3 cut(s) 437, 568, 650
AspS9I GGNCC 3 cut(s) 328, 759, 760
AsuHPI GGTGA 3 cut(s) 280, 701, 1055
AvaII GGWCC 1 cut(s) 328
BaeGI GKGCMC 1 cut(s) 763
BamHI GGATCC 1 cut(s) 956
BanII GRGCYC 1 cut(s) 763
BauI CACGAG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 9 cut(s) 149, 449, 522, 529, 555, 654, 657, 676, 941
BccI CCATC 5 cut(s) 50, 311, 890, 956, 982
BceAI ACGGC 2 cut(s) 150, 427
BciT130I CCWGG 1 cut(s) 325
BclI TGATCA 1 cut(s) 492
BfoI RGCGCY 2 cut(s) 569, 651
BfuAI ACCTGC 2 cut(s) 266, 360
BglII AGATCT 1 cut(s) 773
BlpI GCTNAGC 1 cut(s) 431
Bme1390I CCNGG 1 cut(s) 325
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 3 cut(s) 328, 759, 760
BmiI GGNNCC 5 cut(s) 329, 522, 760, 761, 958
BmrFI CCNGG 1 cut(s) 325
BmsI GCATC 3 cut(s) 209, 509, 748
Bpu10I CCTNAGC 1 cut(s) 602
Bpu1102I GCTNAGC 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 4 cut(s) 324, 409, 724, 849
BsaWI WCCGGW 3 cut(s) 208, 523, 711
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 890
Bse118I RCCGGY 3 cut(s) 531, 650, 711
Bse1I ACTGG 1 cut(s) 602
BseAI TCCGGA 2 cut(s) 208, 523
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 4 cut(s) 324, 409, 724, 849
BseGI GGATG 2 cut(s) 200, 901
BseLI CCNNNNNNNGG 2 cut(s) 115, 890
BseMII CTCAG 2 cut(s) 768, 803
BseNI ACTGG 1 cut(s) 602
BseRI GAGGAG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 763
BseXI GCAGC 9 cut(s) 149, 449, 522, 529, 555, 654, 657, 676, 941
BseYI CCCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 554
Bsh1236I CGCG 2 cut(s) 557, 864
BshFI GGCC 1 cut(s) 761
BshTI ACCGGT 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 531
BsiSI CCGG 5 cut(s) 209, 524, 532, 651, 712
BslFI GGGAC 4 cut(s) 284, 470, 620, 690
BslI CCNNNNNNNGG 2 cut(s) 115, 890
BsmFI GGGAC 4 cut(s) 284, 470, 620, 690
BsnI GGCC 1 cut(s) 761
Bsp120I GGGCCC 1 cut(s) 759
Bsp1286I GDGCHC 2 cut(s) 531, 763
Bsp13I TCCGGA 2 cut(s) 208, 523
Bsp1720I GCTNAGC 1 cut(s) 431
Bsp19I CCATGG 1 cut(s) 849
BspANI GGCC 1 cut(s) 761
BspCNI CTCAG 2 cut(s) 769, 804
BspEI TCCGGA 2 cut(s) 208, 523
BspFNI CGCG 2 cut(s) 557, 864
BspLI GGNNCC 5 cut(s) 329, 522, 760, 761, 958
BspMI ACCTGC 2 cut(s) 266, 360
BspPI GGATC 7 cut(s) 170, 257, 715, 924, 951, 964, 1083
BsrBI CCGCTC 1 cut(s) 394
BsrFI RCCGGY 3 cut(s) 531, 650, 711
BsrI ACTGG 1 cut(s) 602
BssAI RCCGGY 3 cut(s) 531, 650, 711
BssECI CCNNGG 4 cut(s) 324, 409, 724, 849
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 1 cut(s) 849
Bst2BI CACGAG 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 3 cut(s) 163, 747, 840
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 535
BstC8I GCNNGC 3 cut(s) 381, 519, 540
BstDEI CTNAG 4 cut(s) 431, 602, 777, 812
BstDSI CCRYGG 2 cut(s) 409, 849
BstF5I GGATG 2 cut(s) 200, 901
BstFNI CGCG 2 cut(s) 557, 864
BstH2I RGCGCY 2 cut(s) 569, 651
BstHHI GCGC 3 cut(s) 437, 568, 650
BstMWI GCNNNNNNNGC 7 cut(s) 385, 535, 563, 654, 671, 695, 861
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 323
BstSLI GKGCMC 1 cut(s) 763
BstUI CGCG 2 cut(s) 557, 864
BstV1I GCAGC 9 cut(s) 149, 449, 522, 529, 555, 654, 657, 676, 941
BstX2I RGATCY 4 cut(s) 720, 773, 956, 1075
BstXI CCANNNNNNTGG 1 cut(s) 597
BstYI RGATCY 4 cut(s) 720, 773, 956, 1075
BsuRI GGCC 1 cut(s) 761
BtgI CCRYGG 2 cut(s) 409, 849
BtgZI GCGATG 1 cut(s) 481
BtsCI GGATG 2 cut(s) 200, 901
BtsIMutI CAGTG 1 cut(s) 760
BveI ACCTGC 2 cut(s) 266, 360
Cac8I GCNNGC 3 cut(s) 381, 519, 540
CfoI GCGC 3 cut(s) 437, 568, 650
Cfr10I RCCGGY 3 cut(s) 531, 650, 711
Cfr13I GGNCC 3 cut(s) 328, 759, 760
CseI GACGC 2 cut(s) 454, 563
Csp6I GTAC 2 cut(s) 403, 511
CspAI ACCGGT 1 cut(s) 711
CviAII CATG 5 cut(s) 637, 671, 850, 869, 1058
CviQI GTAC 2 cut(s) 403, 511
DdeI CTNAG 4 cut(s) 431, 602, 777, 812
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 849
Eco24I GRGCYC 1 cut(s) 763
Eco47I GGWCC 1 cut(s) 328
Eco47III AGCGCT 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 759
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 849
EcoT38I GRGCYC 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 849
FaeI CATG 5 cut(s) 640, 674, 853, 872, 1061
FaqI GGGAC 4 cut(s) 284, 470, 620, 690
FatI CATG 5 cut(s) 636, 670, 849, 868, 1057
FauI CCCGC 6 cut(s) 353, 384, 388, 410, 547, 667
FbaI TGATCA 1 cut(s) 492
FokI GGATG 2 cut(s) 187, 908
FriOI GRGCYC 1 cut(s) 763
GlaI GCGC 3 cut(s) 436, 567, 649
GsaI CCCAGC 1 cut(s) 254
HaeII RGCGCY 2 cut(s) 569, 651
HaeIII GGCC 1 cut(s) 761
HapII CCGG 5 cut(s) 209, 524, 532, 651, 712
HgaI GACGC 2 cut(s) 454, 563
HhaI GCGC 3 cut(s) 437, 568, 650
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 5 cut(s) 640, 674, 853, 872, 1061
Hin6I GCGC 3 cut(s) 435, 566, 648
HinP1I GCGC 3 cut(s) 435, 566, 648
HincII GTYRAC 2 cut(s) 469, 859
HindII GTYRAC 2 cut(s) 469, 859
HinfI GANTC 3 cut(s) 38, 779, 936
HpaII CCGG 5 cut(s) 209, 524, 532, 651, 712
HphI GGTGA 3 cut(s) 280, 701, 1055
Hpy166II GTNNAC 3 cut(s) 403, 469, 859
Hpy188I TCNGA 6 cut(s) 332, 725, 778, 828, 961, 987
Hpy188III TCNNGA 6 cut(s) 46, 116, 173, 209, 242, 524
Hpy8I GTNNAC 3 cut(s) 403, 469, 859
HpyAV CCTTC 4 cut(s) 281, 919, 1003, 1040
HpyCH4III ACNGT 3 cut(s) 163, 747, 840
HpyCH4IV ACGT 2 cut(s) 297, 803
HpyCH4V TGCA 4 cut(s) 200, 571, 739, 879
HpyF10VI GCNNNNNNNGC 7 cut(s) 385, 535, 563, 654, 671, 695, 861
HpyF3I CTNAG 4 cut(s) 431, 602, 777, 812
HpySE526I ACGT 2 cut(s) 297, 803
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 5 cut(s) 640, 674, 853, 872, 1061
HspAI GCGC 3 cut(s) 435, 566, 648
Kpn2I TCCGGA 2 cut(s) 208, 523
Ksp22I TGATCA 1 cut(s) 492
LmnI GCTCC 2 cut(s) 526, 526
Lsp1109I GCAGC 9 cut(s) 149, 449, 522, 529, 555, 654, 657, 676, 941
LweI GCATC 3 cut(s) 209, 509, 748
MaeII ACGT 2 cut(s) 297, 803
MaeIII GTNAC 1 cut(s) 629
MbiI CCGCTC 1 cut(s) 394
MboII GAAGA 5 cut(s) 730, 742, 1012, 1098, 1101
MfeI CAATTG 1 cut(s) 920
MflI RGATCY 4 cut(s) 720, 773, 956, 1075
MhlI GDGCHC 2 cut(s) 531, 763
MluCI AATT 4 cut(s) 10, 236, 625, 920
MlyI GAGTC 2 cut(s) 32, 788
MmeI TCCRAC 2 cut(s) 768, 894
MnlI CCTC 8 cut(s) 200, 376, 451, 544, 644, 719, 985, 1043
MroI TCCGGA 2 cut(s) 208, 523
MseI TTAA 2 cut(s) 9, 32
MslI CAYNNNNRTG 1 cut(s) 1041
MspA1I CMGCKG 3 cut(s) 542, 811, 932
MspI CCGG 5 cut(s) 209, 524, 532, 651, 712
MspR9I CCNGG 1 cut(s) 325
MunI CAATTG 1 cut(s) 920
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 2 cut(s) 557, 864
MwoI GCNNNNNNNGC 7 cut(s) 385, 535, 563, 654, 671, 695, 861
NcoI CCATGG 1 cut(s) 849
NlaIII CATG 5 cut(s) 640, 674, 853, 872, 1061
NlaIV GGNNCC 5 cut(s) 329, 522, 760, 761, 958
PaqCI CACCTGC 2 cut(s) 266, 360
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 462
PfeI GAWTC 1 cut(s) 936
PinAI ACCGGT 1 cut(s) 711
PleI GAGTC 2 cut(s) 32, 787
PpsI GAGTC 2 cut(s) 32, 787
Psp6I CCWGG 1 cut(s) 323
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 5 cut(s) 329, 522, 760, 761, 958
PspOMI GGGCCC 1 cut(s) 759
PspPI GGNCC 3 cut(s) 328, 759, 760
PsuI RGATCY 4 cut(s) 720, 773, 956, 1075
PvuII CAGCTG 1 cut(s) 932
RsaI GTAC 2 cut(s) 404, 512
RsaNI GTAC 2 cut(s) 403, 511
RseI CAYNNNNRTG 1 cut(s) 1041
SaqAI TTAA 2 cut(s) 9, 32
Sau96I GGNCC 3 cut(s) 328, 759, 760
SchI GAGTC 2 cut(s) 32, 788
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 2 cut(s) 531, 763
SfaNI GCATC 3 cut(s) 209, 509, 748
SgrAI CRCCGGYG 1 cut(s) 650
SinI GGWCC 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 1041
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 4 cut(s) 10, 236, 625, 920
SspI AATATT 2 cut(s) 103, 336
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 1 cut(s) 849
TaaI ACNGT 3 cut(s) 163, 747, 840
TaiI ACGT 2 cut(s) 300, 806
TaqI TCGA 4 cut(s) 14, 165, 357, 623
TasI AATT 4 cut(s) 10, 236, 625, 920
TatI WGTACW 2 cut(s) 402, 510
TauI GCSGC 3 cut(s) 417, 689, 811
TfiI GAWTC 1 cut(s) 936
Tru1I TTAA 2 cut(s) 9, 32
Tru9I TTAA 2 cut(s) 9, 32
TscAI CASTG 1 cut(s) 760
TseI GCWGC 9 cut(s) 137, 437, 535, 542, 568, 642, 645, 689, 929
TspDTI ATGAA 2 cut(s) 412, 1101
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 328
XapI RAATTY 1 cut(s) 236
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.