Prupe.2G286700_v2.0.a1

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000860
Physical Location & Seq
Forward (+)
28268887 .. 28270521
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G286700.1

Sequence Viewer

Length: 993 bp
ATGTCAGGTGCAGGCGAAGGTGAAGCAGAAGACATCTCCACCGTTGATAACTATCAGCGCCTCCAGATTGCACCCAACCCTGACGGCACCATCACCCGCTTGGCCAAATTCCCAGAAAGCCCACCCACGTCCGATCCCAAGCTTCCCACCCCAGTTCTCTCCAAAGACATCCCCATCAACGAATCAAAAAACACCTGGGTTCGAATCTATTTGCCCCGCCACGCACTGGATCATTCCTCCTCCTCCAAGTTACCTCTCGTCGTTTTTTACCATGGTGGAGGCTTCATTTTATTCAGTGCAGCCTCAACCATATTCCACGATTACTGCGTCGGCTTCGCCACTGATGTTCCCGTCATCATCGCATCGGTCGAGTACAGGCTCGCTCCAGAGCATCGCCTGCCAGCGGCCTATGAGGATGCAACGGAAGCGCTGCACTGGATCAAAACCACCCCAGATGATTGGTTGAGAGACCATGCTGACCTTTCAAATACTTTCCTCATGGGCATCAGCGCGGGGGGTAACATAGCCTACCACGCAGCACTTCACGCCGACGTTGACCGTCTCCATCCGTTGAAGATCCATGGGCTGATATTACAGCAACCGTTTTTCAGTGGGACCCAGAGGAGTGGCTCCGAGTTGAGGTTGGCGAACAACCCCGCGTTCCCACTGAGCTGTTCGGATCTGATGTGGGACTTGTCGTTGCCTATTGGGGCTAGTCGTGACCACGAGTATTGCAATCCGACGGTAGGGGACGGTTGTAAGAAGTTGGATCGAATGGCGGTTGGGTGGAGAGTTTTGGTGACTGGCTGGGATGGGGACCCATTGATCGACCGTCAGATTGAGGTGGCCAAGATGTTGGAGGAAAATGGTGTACACGTGGTGTGCCATTTTGCTGAAGGAGGTTACCATGGGGTTGATATTATTGACGCCTCCAAGGCCGAGGCACTGTTTGTGGTTGTTAAGAATTTCATATTTTCTCAATCGGCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

331

Amino Acids

36.2

Weight (kDa)

5.36

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 226
AccII CGCG 2 cut(s) 512, 659
AciI CCGC 6 cut(s) 97, 217, 404, 512, 657, 779
AclWI GGATC 6 cut(s) 128, 237, 446, 571, 687, 777
AcoI YGGCCR 2 cut(s) 102, 846
AcsI RAATTY 2 cut(s) 107, 964
AcuI CTGAAG 1 cut(s) 915
AcvI CACGTG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 927
AdeI CACNNNGTG 1 cut(s) 880
AfaI GTAC 2 cut(s) 374, 873
AfeI AGCGCT 1 cut(s) 429
AfiI CCNNNNNNNGG 4 cut(s) 226, 403, 639, 746
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 2 cut(s) 486, 574
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 2 cut(s) 142, 672
AluI AGCT 2 cut(s) 142, 672
Alw26I GTCTC 2 cut(s) 462, 566
AlwI GGATC 6 cut(s) 128, 237, 446, 571, 687, 777
Aor51HI AGCGCT 1 cut(s) 429
AoxI GGCC 4 cut(s) 102, 405, 846, 936
ApeKI GCWGC 3 cut(s) 299, 430, 536
ApoI RAATTY 2 cut(s) 107, 964
AspLEI GCGC 3 cut(s) 60, 430, 512
AspS9I GGNCC 2 cut(s) 615, 817
AsuHPI GGTGA 3 cut(s) 32, 85, 811
AsuII TTCGAA 1 cut(s) 202
AvaII GGWCC 2 cut(s) 615, 817
BalI TGGCCA 2 cut(s) 104, 848
BanI GGYRCC 1 cut(s) 86
BauI CACGAG 1 cut(s) 725
BbrPI CACGTG 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 36
BbvI GCAGC 3 cut(s) 311, 417, 548
BccI CCATC 4 cut(s) 98, 182, 573, 806
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 196
BcoDI GTCTC 2 cut(s) 462, 566
BfaI CTAG 2 cut(s) 714, 987
BfoI RGCGCY 2 cut(s) 61, 431
BglI GCCNNNNNGGC 1 cut(s) 935
BisI GCNGC 4 cut(s) 300, 405, 431, 537
BlsI GCNGC 4 cut(s) 301, 406, 432, 538
Bme1390I CCNGG 1 cut(s) 196
Bme18I GGWCC 2 cut(s) 615, 817
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 615, 817
BmiI GGNNCC 6 cut(s) 88, 616, 617, 631, 818, 819
BmrFI CCNGG 1 cut(s) 196
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 4 cut(s) 371, 400, 406, 513
BmuI ACTGGG 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 36
BpmI CTGGAG 2 cut(s) 47, 369
Bpu14I TTCGAA 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 877
BsaBI GATNNNNATC 1 cut(s) 51
BsaHI GRCGYC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 6 cut(s) 195, 271, 580, 907, 933, 939
Bsc4I CCNNNNNNNGG 4 cut(s) 226, 403, 639, 746
Bse1I ACTGG 4 cut(s) 152, 231, 440, 808
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 6 cut(s) 195, 271, 580, 907, 933, 939
BseGI GGATG 4 cut(s) 168, 421, 565, 817
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 4 cut(s) 226, 403, 639, 746
BseMII CTCAG 1 cut(s) 659
BseNI ACTGG 4 cut(s) 152, 231, 440, 808
BseRI GAGGAG 3 cut(s) 229, 232, 637
BseXI GCAGC 3 cut(s) 311, 417, 548
BseYI CCCAGC 1 cut(s) 807
BsgI GTGCAG 3 cut(s) 30, 318, 416
Bsh1236I CGCG 2 cut(s) 512, 659
Bsh1285I CGRYCG 2 cut(s) 369, 832
BshFI GGCC 4 cut(s) 104, 407, 848, 938
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 369, 832
BslFI GGGAC 4 cut(s) 628, 704, 764, 830
BslI CCNNNNNNNGG 4 cut(s) 226, 403, 639, 746
BsmAI GTCTC 2 cut(s) 462, 566
BsmBI CGTCTC 1 cut(s) 566
BsmFI GGGAC 4 cut(s) 628, 704, 764, 830
BsnI GGCC 4 cut(s) 104, 407, 848, 938
Bso31I GGTCTC 1 cut(s) 462
Bsp119I TTCGAA 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 871
Bsp143I GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
Bsp19I CCATGG 3 cut(s) 271, 580, 907
BspACI CCGC 6 cut(s) 97, 217, 404, 512, 657, 779
BspANI GGCC 4 cut(s) 104, 407, 848, 938
BspCNI CTCAG 1 cut(s) 660
BspFNI CGCG 2 cut(s) 512, 659
BspLI GGNNCC 6 cut(s) 88, 616, 617, 631, 818, 819
BspPI GGATC 6 cut(s) 128, 237, 446, 571, 687, 777
BspT104I TTCGAA 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 462
BsrGI TGTACA 1 cut(s) 871
BsrI ACTGG 4 cut(s) 152, 231, 440, 808
BssECI CCNNGG 6 cut(s) 195, 271, 580, 907, 933, 939
BssMI GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
BssNI GRCGYC 1 cut(s) 927
BssSI CACGAG 1 cut(s) 725
BssT1I CCWWGG 4 cut(s) 271, 580, 907, 933
Bst2BI CACGAG 1 cut(s) 725
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 7 cut(s) 43, 560, 603, 745, 755, 833, 948
BstACI GRCGYC 1 cut(s) 927
BstAPI GCANNNNNTGC 1 cut(s) 397
BstAUI TGTACA 1 cut(s) 871
BstBAI YACGTR 1 cut(s) 877
BstBI TTCGAA 1 cut(s) 202
BstC8I GCNNGC 4 cut(s) 13, 381, 398, 402
BstDEI CTNAG 1 cut(s) 668
BstDSI CCRYGG 3 cut(s) 271, 580, 907
BstEII GGTNACC 1 cut(s) 902
BstF5I GGATG 4 cut(s) 168, 421, 565, 817
BstFNI CGCG 2 cut(s) 512, 659
BstH2I RGCGCY 2 cut(s) 61, 431
BstHHI GCGC 3 cut(s) 60, 430, 512
BstKTI GATC 7 cut(s) 136, 232, 441, 579, 682, 772, 828
BstMAI GTCTC 2 cut(s) 462, 566
BstMBI GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
BstMCI CGRYCG 2 cut(s) 369, 832
BstMWI GCNNNNNNNGC 5 cut(s) 397, 425, 533, 545, 935
BstNI CCWGG 1 cut(s) 196
BstPI GGTNACC 1 cut(s) 902
BstSCI CCNGG 1 cut(s) 194
BstUI CGCG 2 cut(s) 512, 659
BstV1I GCAGC 3 cut(s) 311, 417, 548
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 2 cut(s) 576, 679
BstXI CCANNNNNNTGG 3 cut(s) 459, 626, 856
BstYI RGATCY 2 cut(s) 576, 679
BsuRI GGCC 4 cut(s) 104, 407, 848, 938
BtgI CCRYGG 3 cut(s) 271, 580, 907
BtgZI GCGATG 2 cut(s) 343, 377
BtrI CACGTC 1 cut(s) 129
BtsCI GGATG 4 cut(s) 168, 421, 565, 817
BtsIMutI CAGTG 7 cut(s) 224, 301, 339, 433, 616, 665, 944
Cac8I GCNNGC 4 cut(s) 13, 381, 398, 402
CfoI GCGC 3 cut(s) 60, 430, 512
Cfr13I GGNCC 2 cut(s) 615, 817
CseI GACGC 2 cut(s) 316, 935
Csp6I GTAC 2 cut(s) 373, 872
CspCI CAANNNNNGTGG 2 cut(s) 713, 748
CviAII CATG 5 cut(s) 272, 473, 499, 581, 908
CviQI GTAC 2 cut(s) 373, 872
DdeI CTNAG 1 cut(s) 668
DpnI GATC 7 cut(s) 135, 231, 440, 578, 681, 771, 827
DpnII GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
DraIII CACNNNGTG 1 cut(s) 880
EaeI YGGCCR 2 cut(s) 102, 846
Eco130I CCWWGG 4 cut(s) 271, 580, 907, 933
Eco31I GGTCTC 1 cut(s) 462
Eco47I GGWCC 2 cut(s) 615, 817
Eco47III AGCGCT 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 915
Eco72I CACGTG 1 cut(s) 877
Eco91I GGTNACC 1 cut(s) 902
EcoO109I RGGNCCY 2 cut(s) 615, 817
EcoO65I GGTNACC 1 cut(s) 902
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 4 cut(s) 271, 580, 907, 933
ErhI CCWWGG 4 cut(s) 271, 580, 907, 933
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 5 cut(s) 275, 476, 502, 584, 911
FaiI YATR 9 cut(s) 273, 311, 411, 474, 500, 524, 582, 909, 971
FaqI GGGAC 4 cut(s) 628, 704, 764, 830
FatI CATG 5 cut(s) 271, 472, 498, 580, 907
FauI CCCGC 4 cut(s) 104, 224, 505, 664
Fnu4HI GCNGC 4 cut(s) 300, 405, 431, 537
FokI GGATG 4 cut(s) 155, 428, 552, 824
Fsp4HI GCNGC 4 cut(s) 300, 405, 431, 537
FspBI CTAG 2 cut(s) 714, 987
GlaI GCGC 3 cut(s) 59, 429, 511
GluI GCNGC 4 cut(s) 300, 405, 431, 537
GsaI CCCAGC 1 cut(s) 811
GsuI CTGGAG 2 cut(s) 47, 369
HaeII RGCGCY 2 cut(s) 61, 431
HaeIII GGCC 4 cut(s) 104, 407, 848, 938
HgaI GACGC 2 cut(s) 316, 935
HhaI GCGC 3 cut(s) 60, 430, 512
Hin1I GRCGYC 1 cut(s) 927
Hin1II CATG 5 cut(s) 275, 476, 502, 584, 911
Hin6I GCGC 3 cut(s) 58, 428, 510
HinP1I GCGC 3 cut(s) 58, 428, 510
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 2 cut(s) 182, 204
HphI GGTGA 3 cut(s) 32, 85, 811
Hpy166II GTNNAC 3 cut(s) 556, 872, 874
Hpy188I TCNGA 6 cut(s) 133, 634, 679, 684, 741, 837
Hpy188III TCNNGA 3 cut(s) 64, 386, 719
Hpy8I GTNNAC 3 cut(s) 556, 872, 874
Hpy99I CGWCG 4 cut(s) 263, 332, 554, 745
HpyAV CCTTC 2 cut(s) 11, 890
HpyCH4III ACNGT 7 cut(s) 43, 560, 603, 745, 755, 833, 948
HpyCH4IV ACGT 3 cut(s) 128, 552, 876
HpyCH4V TGCA 6 cut(s) 11, 71, 299, 419, 433, 735
HpyF10VI GCNNNNNNNGC 5 cut(s) 397, 425, 533, 545, 935
HpyF3I CTNAG 1 cut(s) 668
HpySE526I ACGT 3 cut(s) 128, 552, 876
Hsp92I GRCGYC 1 cut(s) 927
Hsp92II CATG 5 cut(s) 275, 476, 502, 584, 911
HspAI GCGC 3 cut(s) 58, 428, 510
KflI GGGWCCC 2 cut(s) 615, 817
Kzo9I GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
LmnI GCTCC 2 cut(s) 388, 635
Lsp1109I GCAGC 3 cut(s) 311, 417, 548
LweI GCATC 4 cut(s) 371, 400, 406, 513
MaeI CTAG 2 cut(s) 714, 987
MaeII ACGT 3 cut(s) 128, 552, 876
MaeIII GTNAC 5 cut(s) 249, 518, 719, 799, 902
MalI GATC 7 cut(s) 135, 231, 440, 578, 681, 771, 827
MboI GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
MboII GAAGA 2 cut(s) 41, 586
MflI RGATCY 2 cut(s) 576, 679
MlsI TGGCCA 2 cut(s) 104, 848
MluCI AATT 2 cut(s) 107, 964
MluNI TGGCCA 2 cut(s) 104, 848
MmeI TCCRAC 3 cut(s) 747, 764, 837
Mox20I TGGCCA 2 cut(s) 104, 848
MscI TGGCCA 2 cut(s) 104, 848
MseI TTAA 1 cut(s) 960
Msp20I TGGCCA 2 cut(s) 104, 848
MspA1I CMGCKG 1 cut(s) 404
MspR9I CCNGG 1 cut(s) 196
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 2 cut(s) 512, 659
MwoI GCNNNNNNNGC 5 cut(s) 397, 425, 533, 545, 935
NcoI CCATGG 3 cut(s) 271, 580, 907
NdeII GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
NlaIII CATG 5 cut(s) 275, 476, 502, 584, 911
NlaIV GGNNCC 6 cut(s) 88, 616, 617, 631, 818, 819
NmeAIII GCCGAG 1 cut(s) 964
NmuCI GTSAC 2 cut(s) 719, 799
NspV TTCGAA 1 cut(s) 202
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 366
PfeI GAWTC 2 cut(s) 182, 204
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 4 cut(s) 301, 406, 432, 538
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
Ppu21I YACGTR 1 cut(s) 877
PpuMI RGGWCCY 2 cut(s) 615, 817
Psp5II RGGWCCY 2 cut(s) 615, 817
Psp6I CCWGG 1 cut(s) 194
PspCI CACGTG 1 cut(s) 877
PspEI GGTNACC 1 cut(s) 902
PspFI CCCAGC 1 cut(s) 807
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 6 cut(s) 88, 616, 617, 631, 818, 819
PspPI GGNCC 2 cut(s) 615, 817
PspPPI RGGWCCY 2 cut(s) 615, 817
PsuI RGATCY 2 cut(s) 576, 679
RsaI GTAC 2 cut(s) 374, 873
RsaNI GTAC 2 cut(s) 373, 872
SaqAI TTAA 1 cut(s) 960
SatI GCNGC 4 cut(s) 300, 405, 431, 537
Sau3AI GATC 7 cut(s) 133, 229, 438, 576, 679, 769, 825
Sau96I GGNCC 2 cut(s) 615, 817
ScrFI CCNGG 1 cut(s) 196
SfaNI GCATC 4 cut(s) 371, 400, 406, 513
SfuI TTCGAA 1 cut(s) 202
SinI GGWCC 2 cut(s) 615, 817
Sse9I AATT 2 cut(s) 107, 964
SsiI CCGC 6 cut(s) 97, 217, 404, 512, 657, 779
SspMI CTAG 2 cut(s) 714, 987
StyD4I CCNGG 1 cut(s) 194
StyI CCWWGG 4 cut(s) 271, 580, 907, 933
TaaI ACNGT 7 cut(s) 43, 560, 603, 745, 755, 833, 948
TaiI ACGT 3 cut(s) 131, 555, 879
TaqI TCGA 4 cut(s) 202, 369, 772, 828
TaqII GACCGA 1 cut(s) 355
TasI AATT 2 cut(s) 107, 964
TatI WGTACW 2 cut(s) 372, 871
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 2 cut(s) 182, 204
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 7 cut(s) 231, 301, 346, 440, 616, 672, 951
TseFI GTSAC 2 cut(s) 719, 799
TseI GCWGC 3 cut(s) 299, 430, 536
Tsp45I GTSAC 2 cut(s) 719, 799
TspDTI ATGAA 2 cut(s) 274, 958
TspGWI ACGGA 2 cut(s) 437, 558
TspRI CASTG 7 cut(s) 231, 301, 346, 440, 616, 672, 951
Van91I CCANNNNNTGG 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 615, 817
XapI RAATTY 2 cut(s) 107, 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XspI CTAG 2 cut(s) 714, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.