Rh7CG442500

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000860
Physical Location & Seq
Reverse (-)
57990603 .. 58023488
32886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG442500.1

Sequence Viewer

Length: 621 bp
ATGACAGACAGAGTCTACCTGTCTTCCAAGGTTGCCACGAGCGGCGCCGCCGCTGCCAACCCTACAGCCGTACCGCCACCATCGCCACTAACCCGAGTTCACCGTCACACCGCGGCCGCAATGGGAGTTCGTGGGTCAAGTCCAAGCCGGGTACTGCTGATGTGTTATCCTGGTGGTTCGGTTCGGATGATGAAGAAGACTCGCAGAAGAAGAAATGGTCTGGAGGTAGACTTGATCTGCGCCTGCAAGCAACATCAGAAGTTCTATGCTGGTAAGTGTCACAGCACAGATGGACATGGAGGAAGAAGAACCCATATTCCTTTAGGGGCACAATCCAAGATAGATGGTGAGTTTATTTGGGCTGCTGCTGTTCGCATTCAAGCATCCAAGGTCAGCAACAAATTCAAGTCAAGAATTAAGGTGTCCTTAAAAACCTGTGGGGTGCACCTTGTAATACCACCCAATGAAGACGTTTGTATGAAGCTATCCCATGCAGAGAACCTCAACAATGAATTTCCATCTGACCTTTACCAGTTATGTCAGTATACCGAAGATGTAAACATAGTTCTTCCAGGTGACAGCCTGGTGACCGCTACTAGGGGGTGGACTATTAGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

206

Amino Acids

22.51

Weight (kDa)

9.87

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 3 cut(s) 15, 228, 545
AccII CGCG 1 cut(s) 113
AciI CCGC 8 cut(s) 42, 48, 51, 74, 111, 113, 117, 591
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 2 cut(s) 401, 512
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 72, 153
AfiI CCNNNNNNNGG 1 cut(s) 597
AgsI TTSAA 2 cut(s) 380, 406
AjnI CCWGG 3 cut(s) 169, 571, 582
AloI GAACNNNNNNTCC 2 cut(s) 301, 333
AluBI AGCT 1 cut(s) 484
AluI AGCT 1 cut(s) 484
Alw21I GWGCWC 1 cut(s) 447
Alw44I GTGCAC 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 1 cut(s) 114
ApaLI GTGCAC 1 cut(s) 443
ApeKI GCWGC 3 cut(s) 53, 362, 365
ApoI RAATTY 2 cut(s) 401, 512
AspLEI GCGC 2 cut(s) 47, 242
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 4 cut(s) 92, 359, 587, 598
AvaI CYCGRG 1 cut(s) 93
BaeGI GKGCMC 2 cut(s) 331, 447
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BanI GGYRCC 1 cut(s) 44
BauI CACGAG 1 cut(s) 37
BbsI GAAGAC 3 cut(s) 15, 203, 474
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 3 cut(s) 40, 349, 352
BccI CCATC 4 cut(s) 88, 284, 338, 526
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 3 cut(s) 171, 573, 584
BcnI CCSGG 1 cut(s) 149
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 48
BisI GCNGC 8 cut(s) 43, 48, 51, 54, 114, 117, 363, 366
BlsI GCNGC 8 cut(s) 44, 49, 52, 55, 115, 118, 364, 367
Bme1390I CCNGG 4 cut(s) 149, 171, 573, 584
BmeT110I CYCGRG 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 4 cut(s) 149, 171, 573, 584
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 3 cut(s) 15, 203, 474
BpmI CTGGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 149
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 3 cut(s) 27, 111, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 597
Bse1I ACTGG 1 cut(s) 532
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 3 cut(s) 171, 573, 584
BseDI CCNNGG 3 cut(s) 27, 111, 387
BseGI GGATG 2 cut(s) 192, 383
BseLI CCNNNNNNNGG 1 cut(s) 597
BseMI GCAATG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 532
BseSI GKGCMC 2 cut(s) 331, 447
BseX3I CGGCCG 1 cut(s) 114
BseXI GCAGC 3 cut(s) 40, 349, 352
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 116
BshNI GGYRCC 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 597
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 331, 447
Bsp143I GATC 1 cut(s) 234
BspACI CCGC 8 cut(s) 42, 48, 51, 74, 111, 113, 117, 591
BspANI GGCC 1 cut(s) 116
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 1 cut(s) 532
BssECI CCNNGG 3 cut(s) 27, 111, 387
BssMI GATC 1 cut(s) 234
BssNAI GTATAC 1 cut(s) 546
BssNI GRCGYC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 37
BssT1I CCWWGG 2 cut(s) 27, 387
Bst1107I GTATAC 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 37
Bst2UI CCWGG 3 cut(s) 171, 573, 584
Bst4CI ACNGT 1 cut(s) 104
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 244, 248
BstDSI CCRYGG 1 cut(s) 111
BstEII GGTNACC 1 cut(s) 586
BstF5I GGATG 2 cut(s) 192, 383
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 2 cut(s) 47, 242
BstKTI GATC 1 cut(s) 237
BstMBI GATC 1 cut(s) 234
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 2 cut(s) 53, 82
BstNI CCWGG 3 cut(s) 171, 573, 584
BstPI GGTNACC 1 cut(s) 586
BstSCI CCNGG 4 cut(s) 147, 169, 571, 582
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 2 cut(s) 331, 447
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 40, 349, 352
BstV2I GAAGAC 3 cut(s) 15, 203, 474
BstZ17I GTATAC 1 cut(s) 546
BstZI CGGCCG 1 cut(s) 114
BsuRI GGCC 1 cut(s) 116
BtgI CCRYGG 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 192, 383
Cac8I GCNNGC 2 cut(s) 244, 248
CciNI GCGGCCGC 1 cut(s) 114
CfoI GCGC 2 cut(s) 47, 242
Cfr42I CCGCGG 1 cut(s) 114
Csp6I GTAC 2 cut(s) 71, 152
CviAII CATG 2 cut(s) 296, 491
CviJI RGCY 7 cut(s) 68, 116, 147, 362, 484, 582, 617
CviKI_1 RGCY 7 cut(s) 68, 116, 147, 362, 484, 582, 617
CviQI GTAC 2 cut(s) 71, 152
DinI GGCGCC 1 cut(s) 46
DpnI GATC 1 cut(s) 236
DpnII GATC 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
EclXI CGGCCG 1 cut(s) 114
Eco130I CCWWGG 2 cut(s) 27, 387
Eco52I CGGCCG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 93
Eco91I GGTNACC 1 cut(s) 586
EcoO65I GGTNACC 1 cut(s) 586
EcoRII CCWGG 3 cut(s) 169, 571, 582
EcoT14I CCWWGG 2 cut(s) 27, 387
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 2 cut(s) 27, 387
FaeI CATG 2 cut(s) 299, 494
FaiI YATR 8 cut(s) 267, 297, 315, 479, 492, 538, 546, 563
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FatI CATG 2 cut(s) 295, 490
FblI GTMKAC 3 cut(s) 15, 228, 545
Fnu4HI GCNGC 8 cut(s) 43, 48, 51, 54, 114, 117, 363, 366
FokI GGATG 2 cut(s) 199, 370
Fsp4HI GCNGC 8 cut(s) 43, 48, 51, 54, 114, 117, 363, 366
FspBI CTAG 1 cut(s) 597
GlaI GCGC 2 cut(s) 46, 241
GluI GCNGC 8 cut(s) 43, 48, 51, 54, 114, 117, 363, 366
GsuI CTGGAG 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 1 cut(s) 116
HapII CCGG 1 cut(s) 148
HhaI GCGC 2 cut(s) 47, 242
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 2 cut(s) 299, 494
Hin6I GCGC 2 cut(s) 45, 240
HinP1I GCGC 2 cut(s) 45, 240
HinfI GANTC 2 cut(s) 12, 199
HpaII CCGG 1 cut(s) 148
HphI GGTGA 4 cut(s) 92, 359, 587, 598
Hpy166II GTNNAC 7 cut(s) 16, 100, 229, 445, 546, 559, 606
Hpy188I TCNGA 3 cut(s) 186, 258, 523
Hpy188III TCNNGA 2 cut(s) 221, 411
Hpy8I GTNNAC 7 cut(s) 16, 100, 229, 445, 546, 559, 606
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 3 cut(s) 246, 445, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 82
HpySE526I ACGT 1 cut(s) 471
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 2 cut(s) 299, 494
HspAI GCGC 2 cut(s) 45, 240
KasI GGCGCC 1 cut(s) 44
KspI CCGCGG 1 cut(s) 114
Kzo9I GATC 1 cut(s) 234
Lsp1109I GCAGC 3 cut(s) 40, 349, 352
LweI GCATC 1 cut(s) 392
MaeI CTAG 1 cut(s) 597
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 4 cut(s) 104, 278, 575, 586
MalI GATC 1 cut(s) 236
MbiI CCGCTC 1 cut(s) 42
MboI GATC 1 cut(s) 234
MhlI GDGCHC 2 cut(s) 331, 447
MluCI AATT 3 cut(s) 401, 414, 512
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 2 cut(s) 21, 193
MnlI CCTC 3 cut(s) 217, 293, 512
MseI TTAA 2 cut(s) 417, 428
MspA1I CMGCKG 2 cut(s) 53, 113
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 4 cut(s) 149, 171, 573, 584
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 3 cut(s) 171, 573, 584
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 53, 82
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 149
NdeII GATC 1 cut(s) 234
NlaIII CATG 2 cut(s) 299, 494
NlaIV GGNNCC 1 cut(s) 46
NmuCI GTSAC 4 cut(s) 104, 278, 575, 586
NotI GCGGCCGC 1 cut(s) 114
PctI GAATGC 1 cut(s) 375
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 8 cut(s) 44, 49, 52, 55, 115, 118, 364, 367
PleI GAGTC 2 cut(s) 20, 193
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 2 cut(s) 20, 193
Psp6I CCWGG 3 cut(s) 169, 571, 582
PspEI GGTNACC 1 cut(s) 586
PspGI CCWGG 3 cut(s) 169, 571, 582
PspN4I GGNNCC 1 cut(s) 46
PsrI GAACNNNNNNTAC 2 cut(s) 549, 581
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 2 cut(s) 72, 153
RsaNI GTAC 2 cut(s) 71, 152
SacII CCGCGG 1 cut(s) 114
SaqAI TTAA 2 cut(s) 417, 428
SatI GCNGC 8 cut(s) 43, 48, 51, 54, 114, 117, 363, 366
Sau3AI GATC 1 cut(s) 234
SchI GAGTC 2 cut(s) 21, 193
ScrFI CCNGG 4 cut(s) 149, 171, 573, 584
SduI GDGCHC 2 cut(s) 331, 447
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 63
SfoI GGCGCC 1 cut(s) 46
Sfr303I CCGCGG 1 cut(s) 114
SgrBI CCGCGG 1 cut(s) 114
Sse9I AATT 3 cut(s) 401, 414, 512
SsiI CCGC 8 cut(s) 42, 48, 51, 74, 111, 113, 117, 591
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 597
StyD4I CCNGG 4 cut(s) 147, 169, 571, 582
StyI CCWWGG 2 cut(s) 27, 387
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 474
TasI AATT 3 cut(s) 401, 414, 512
TauI GCSGC 5 cut(s) 45, 50, 53, 116, 119
Tru1I TTAA 2 cut(s) 417, 428
Tru9I TTAA 2 cut(s) 417, 428
TseFI GTSAC 4 cut(s) 104, 278, 575, 586
TseI GCWGC 3 cut(s) 53, 362, 365
Tsp45I GTSAC 4 cut(s) 104, 278, 575, 586
TspDTI ATGAA 4 cut(s) 206, 480, 494, 525
Tth111I GACNNNGTC 1 cut(s) 11
VneI GTGCAC 1 cut(s) 443
XapI RAATTY 2 cut(s) 401, 512
XmiI GTMKAC 3 cut(s) 15, 228, 545
XspI CTAG 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.