Rw1G036300

Carboxylesterase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000860
Physical Location & Seq
Reverse (-)
64211700 .. 64214737
3038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G036300.1

Sequence Viewer

Length: 933 bp
ATGTCAAATGAAAGGGCAGCAGCGGATTCCGATCCCAACTCCGGCGTTGATCCTTACCAGCGCCTCCAGATAGCACGCAATCCCGACGGCAGCATCACGCGCTTGTTCAAATGCCCAGACGCCCCGCCCTCATCTGATCCCAAGCTTCCCACTCCAGTTCTCACCAAAGACATCCCCATCAACCAATCAAATAACACCACTTCGACTGTTCCTCCCCCGCCATGTACTGGACCAAATCTCACCAACTACCAAGCTCCCTCTCGTCGTTTATTACCATGGTGGAGGACTGGAGGCTTCATATTACTTAGCGCAGCCTCAACCATTTTCCATGATTTCTGTTTCAACTTTTCAGCCGGCGTCCCCGTGATCGTCGCATCTGTCGAGTACAGGTTGGCTCCTGAGCACCGGCTGCCGGCGGCTTATGATGATATAGTGGAGGCGCTTCACTGGATCAAAACCTGCCAAGATGATTGGTTGAGAGACTACGCTGACCTCTCCAATAGTTTCCTTATGGGATCCAGTGCTGGGGGTAACATAGCCTACAATGCAGGACTTCGTGCAGCTGCTGAGGGCATTGACCTTCTCCACCCGTTGAAGATTAGAGGGCTGATATTGGTGCAACCGTATTTCGGGGGGAATCGAAGGACTAGTTCAGAGTTGACGTCTCATGTGGGACTTTATTGCAATCCAGTGGCGGGTGGCGATTCTAATGGCTTTGATCAAGTGAAAGCACTTGGGTGGAAAATTTTGGTGAGTGGCTCCGATGGAGACCCGCTGATCGATCGTCAGATTGAGCTGGTGAAGATGTTGGAGGCAAAAGGTGTACAAGTGGTGAGTCAATTTATCGAAGGAGGTTATCATGGGGCTCAAGAGGTTGAACCCTCCAAAGCCAAGGATGTGTTTGTGCTTGTTAAACATTTCATATTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

310

Amino Acids

33.73

Weight (kDa)

5.8

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 96 - 289 1e-32 alpha/beta hydrolase fold
BD-FAE PF20434 123 - 192 9.5e-09 BD-FAE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 467
AccB7I CCANNNNNTGG 1 cut(s) 227
AccII CGCG 1 cut(s) 100
AciI CCGC 6 cut(s) 23, 125, 218, 416, 695, 773
AclWI GGATC 6 cut(s) 26, 44, 131, 458, 510, 523
AcsI RAATTY 1 cut(s) 744
AcyI GRCGYC 3 cut(s) 120, 357, 662
AfaI GTAC 3 cut(s) 226, 386, 825
AfiI CCNNNNNNNGG 6 cut(s) 41, 227, 412, 525, 629, 695
AgsI TTSAA 4 cut(s) 109, 343, 595, 878
AhlI ACTAGT 1 cut(s) 647
AleI CACNNNNGTG 1 cut(s) 736
AluBI AGCT 4 cut(s) 145, 254, 563, 796
AluI AGCT 4 cut(s) 145, 254, 563, 796
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 3 cut(s) 474, 669, 762
AlwI GGATC 6 cut(s) 26, 44, 131, 458, 510, 523
AlwNI CAGNNNCTG 1 cut(s) 566
ApeKI GCWGC 7 cut(s) 17, 20, 90, 311, 409, 560, 563
ApoI RAATTY 1 cut(s) 744
AspLEI GCGC 4 cut(s) 63, 102, 311, 442
AspS9I GGNCC 1 cut(s) 230
AsuHPI GGTGA 5 cut(s) 154, 232, 763, 811, 844
AvaII GGWCC 1 cut(s) 230
BamHI GGATCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 868
Bbv12I GWGCWC 1 cut(s) 405
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 7 cut(s) 29, 32, 102, 323, 396, 550, 572
BccI CCATC 2 cut(s) 185, 758
BceAI ACGGC 1 cut(s) 103
BclI TGATCA 1 cut(s) 718
BcoDI GTCTC 3 cut(s) 474, 669, 762
BcuI ACTAGT 1 cut(s) 647
BfaI CTAG 1 cut(s) 648
BfoI RGCGCY 2 cut(s) 64, 443
BfuAI ACCTGC 1 cut(s) 467
BisI GCNGC 8 cut(s) 18, 21, 91, 312, 410, 417, 561, 564
BlsI GCNGC 8 cut(s) 19, 22, 92, 313, 411, 418, 562, 565
Bme18I GGWCC 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 3 cut(s) 396, 517, 760
BmsI GCATC 2 cut(s) 102, 383
BpmI CTGGAG 3 cut(s) 50, 138, 309
Bpu10I CCTNAGC 2 cut(s) 399, 567
BpuEI CTTGAG 1 cut(s) 852
Bsa29I ATCGAT 1 cut(s) 780
BsaBI GATNNNNATC 1 cut(s) 30
BsaHI GRCGYC 3 cut(s) 120, 357, 662
BsaI GGTCTC 1 cut(s) 762
BsaJI CCNNGG 2 cut(s) 275, 891
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 227, 412, 525, 629, 695
Bse118I RCCGGY 3 cut(s) 353, 405, 412
Bse1I ACTGG 6 cut(s) 155, 232, 292, 452, 519, 689
Bse8I GATNNNNATC 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 275, 891
BseGI GGATG 2 cut(s) 171, 901
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 6 cut(s) 41, 227, 412, 525, 629, 695
BseMII CTCAG 2 cut(s) 390, 558
BseNI ACTGG 6 cut(s) 155, 232, 292, 452, 519, 689
BseXI GCAGC 7 cut(s) 29, 32, 102, 323, 396, 550, 572
BseYI CCCAGC 1 cut(s) 524
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 784
BshVI ATCGAT 1 cut(s) 780
BsiEI CGRYCG 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 4 cut(s) 42, 354, 406, 413
BslFI GGGAC 2 cut(s) 344, 687
BslI CCNNNNNNNGG 6 cut(s) 41, 227, 412, 525, 629, 695
BsmAI GTCTC 3 cut(s) 474, 669, 762
BsmBI CGTCTC 1 cut(s) 669
BsmFI GGGAC 2 cut(s) 344, 687
Bso31I GGTCTC 1 cut(s) 762
Bsp1286I GDGCHC 2 cut(s) 405, 868
Bsp1407I TGTACA 1 cut(s) 823
Bsp143I GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 6 cut(s) 23, 125, 218, 416, 695, 773
BspCNI CTCAG 2 cut(s) 391, 559
BspDI ATCGAT 1 cut(s) 780
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 3 cut(s) 396, 517, 760
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 6 cut(s) 26, 44, 131, 458, 510, 523
BspTNI GGTCTC 1 cut(s) 762
BsrFI RCCGGY 3 cut(s) 353, 405, 412
BsrGI TGTACA 1 cut(s) 823
BsrI ACTGG 6 cut(s) 155, 232, 292, 452, 519, 689
BssAI RCCGGY 3 cut(s) 353, 405, 412
BssECI CCNNGG 2 cut(s) 275, 891
BssMI GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
BssNI GRCGYC 3 cut(s) 120, 357, 662
BssT1I CCWWGG 2 cut(s) 275, 891
Bst4CI ACNGT 2 cut(s) 208, 624
BstACI GRCGYC 3 cut(s) 120, 357, 662
BstAPI GCANNNNNTGC 1 cut(s) 409
BstAUI TGTACA 1 cut(s) 823
BstC8I GCNNGC 3 cut(s) 76, 355, 414
BstDEI CTNAG 3 cut(s) 305, 399, 567
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 171, 901
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 64, 443
BstHHI GCGC 4 cut(s) 63, 102, 311, 442
BstKTI GATC 9 cut(s) 34, 52, 139, 369, 453, 518, 721, 780, 784
BstMAI GTCTC 3 cut(s) 474, 669, 762
BstMBI GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
BstMCI CGRYCG 1 cut(s) 784
BstMWI GCNNNNNNNGC 3 cut(s) 99, 409, 545
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 7 cut(s) 29, 32, 102, 323, 396, 550, 572
BstX2I RGATCY 1 cut(s) 515
BstYI RGATCY 1 cut(s) 515
Bsu15I ATCGAT 1 cut(s) 780
BsuTUI ATCGAT 1 cut(s) 780
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 2 cut(s) 171, 901
BtsIMutI CAGTG 3 cut(s) 445, 526, 696
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 3 cut(s) 76, 355, 414
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 4 cut(s) 63, 102, 311, 442
Cfr10I RCCGGY 3 cut(s) 353, 405, 412
Cfr13I GGNCC 1 cut(s) 230
ClaI ATCGAT 1 cut(s) 780
CseI GACGC 2 cut(s) 128, 346
Csp6I GTAC 3 cut(s) 225, 385, 824
CviAII CATG 5 cut(s) 222, 276, 329, 668, 860
CviQI GTAC 3 cut(s) 225, 385, 824
DdeI CTNAG 3 cut(s) 305, 399, 567
DpnI GATC 9 cut(s) 33, 51, 138, 368, 452, 517, 720, 779, 783
DpnII GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
Eco130I CCWWGG 2 cut(s) 275, 891
Eco24I GRGCYC 1 cut(s) 868
Eco31I GGTCTC 1 cut(s) 762
Eco47I GGWCC 1 cut(s) 230
EcoT14I CCWWGG 2 cut(s) 275, 891
EcoT38I GRGCYC 1 cut(s) 868
ErhI CCWWGG 2 cut(s) 275, 891
Esp3I CGTCTC 1 cut(s) 669
FaeI CATG 5 cut(s) 225, 279, 332, 671, 863
FaqI GGGAC 2 cut(s) 344, 687
FatI CATG 5 cut(s) 221, 275, 328, 667, 859
FauI CCCGC 4 cut(s) 132, 225, 688, 780
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 8 cut(s) 18, 21, 91, 312, 410, 417, 561, 564
FokI GGATG 2 cut(s) 158, 908
FriOI GRGCYC 1 cut(s) 868
Fsp4HI GCNGC 8 cut(s) 18, 21, 91, 312, 410, 417, 561, 564
FspBI CTAG 1 cut(s) 648
GlaI GCGC 4 cut(s) 62, 101, 310, 441
GluI GCNGC 8 cut(s) 18, 21, 91, 312, 410, 417, 561, 564
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 3 cut(s) 50, 138, 309
HaeII RGCGCY 2 cut(s) 64, 443
HapII CCGG 4 cut(s) 42, 354, 406, 413
HgaI GACGC 2 cut(s) 128, 346
HhaI GCGC 4 cut(s) 63, 102, 311, 442
Hin1I GRCGYC 3 cut(s) 120, 357, 662
Hin1II CATG 5 cut(s) 225, 279, 332, 671, 863
Hin6I GCGC 4 cut(s) 61, 100, 309, 440
HinP1I GCGC 4 cut(s) 61, 100, 309, 440
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 4 cut(s) 26, 637, 704, 835
HpaII CCGG 4 cut(s) 42, 354, 406, 413
HphI GGTGA 5 cut(s) 154, 232, 763, 811, 844
Hpy166II GTNNAC 2 cut(s) 660, 824
Hpy188I TCNGA 5 cut(s) 31, 136, 655, 763, 789
Hpy188III TCNNGA 4 cut(s) 67, 83, 398, 869
Hpy8I GTNNAC 2 cut(s) 660, 824
Hpy99I CGWCG 3 cut(s) 89, 267, 374
HpyAV CCTTC 3 cut(s) 590, 636, 842
HpyCH4III ACNGT 2 cut(s) 208, 624
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 4 cut(s) 548, 560, 619, 684
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 409, 545
HpyF3I CTNAG 3 cut(s) 305, 399, 567
HpySE526I ACGT 1 cut(s) 662
Hsp92I GRCGYC 3 cut(s) 120, 357, 662
Hsp92II CATG 5 cut(s) 225, 279, 332, 671, 863
HspAI GCGC 4 cut(s) 61, 100, 309, 440
KroI GCCGGC 2 cut(s) 353, 412
KroNI GCCGGC 2 cut(s) 355, 414
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
LmnI GCTCC 3 cut(s) 259, 400, 764
Lsp1109I GCAGC 7 cut(s) 29, 32, 102, 323, 396, 550, 572
LweI GCATC 2 cut(s) 102, 383
MaeI CTAG 1 cut(s) 648
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 1 cut(s) 530
MalI GATC 9 cut(s) 33, 51, 138, 368, 452, 517, 720, 779, 783
MboI GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
MboII GAAGA 2 cut(s) 607, 814
MflI RGATCY 1 cut(s) 515
MhlI GDGCHC 2 cut(s) 405, 868
MluCI AATT 2 cut(s) 744, 839
MlyI GAGTC 1 cut(s) 844
MmeI TCCRAC 1 cut(s) 789
MroNI GCCGGC 2 cut(s) 353, 412
MseI TTAA 2 cut(s) 912, 931
MslI CAYNNNNRTG 1 cut(s) 736
MspA1I CMGCKG 3 cut(s) 23, 563, 775
MspI CCGG 4 cut(s) 42, 354, 406, 413
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 99, 409, 545
NaeI GCCGGC 2 cut(s) 355, 414
NcoI CCATGG 1 cut(s) 275
NdeII GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
NgoMIV GCCGGC 2 cut(s) 353, 412
NlaIII CATG 5 cut(s) 225, 279, 332, 671, 863
NlaIV GGNNCC 3 cut(s) 396, 517, 760
OliI CACNNNNGTG 1 cut(s) 736
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PdiI GCCGGC 2 cut(s) 355, 414
PfeI GAWTC 3 cut(s) 26, 637, 704
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 8 cut(s) 19, 22, 92, 313, 411, 418, 562, 565
Ple19I CGATCG 1 cut(s) 784
PleI GAGTC 1 cut(s) 843
PpsI GAGTC 1 cut(s) 843
PspFI CCCAGC 1 cut(s) 524
PspN4I GGNNCC 3 cut(s) 396, 517, 760
PspPI GGNCC 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 515
PvuI CGATCG 1 cut(s) 784
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 3 cut(s) 226, 386, 825
RsaNI GTAC 3 cut(s) 225, 385, 824
RseI CAYNNNNRTG 1 cut(s) 736
SaqAI TTAA 2 cut(s) 912, 931
SatI GCNGC 8 cut(s) 18, 21, 91, 312, 410, 417, 561, 564
Sau3AI GATC 9 cut(s) 31, 49, 136, 366, 450, 515, 718, 777, 781
Sau96I GGNCC 1 cut(s) 230
SchI GAGTC 1 cut(s) 844
SduI GDGCHC 2 cut(s) 405, 868
SfaNI GCATC 2 cut(s) 102, 383
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 736
SmlI CTYRAG 1 cut(s) 867
SmoI CTYRAG 1 cut(s) 867
SpeI ACTAGT 1 cut(s) 647
Sse9I AATT 2 cut(s) 744, 839
SsiI CCGC 6 cut(s) 23, 125, 218, 416, 695, 773
SspMI CTAG 1 cut(s) 648
StyI CCWWGG 2 cut(s) 275, 891
TaaI ACNGT 2 cut(s) 208, 624
TaiI ACGT 1 cut(s) 665
TaqI TCGA 5 cut(s) 203, 381, 640, 780, 846
TasI AATT 2 cut(s) 744, 839
TatI WGTACW 3 cut(s) 224, 384, 823
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 3 cut(s) 26, 637, 704
Tru1I TTAA 2 cut(s) 912, 931
Tru9I TTAA 2 cut(s) 912, 931
TscAI CASTG 3 cut(s) 452, 526, 696
TseI GCWGC 7 cut(s) 17, 20, 90, 311, 409, 560, 563
TspDTI ATGAA 3 cut(s) 24, 286, 910
TspRI CASTG 3 cut(s) 452, 526, 696
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 230
XapI RAATTY 1 cut(s) 744
XspI CTAG 1 cut(s) 648
ZraI GACGTC 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.