Rroxscaffold_5G00348740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000860
Physical Location & Seq
Reverse (-)
21166077 .. 21177727
11651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348740.1

Sequence Viewer

Length: 546 bp
ATGTGGGATGGCATGCATAATGAGGTGAATGGGCAGAAGGCACATGAAGAAGGTGATGCATGGCAAAACCTCCGCAATCAAACTAGTATTTTCAATCCAGTCCTTCCTTTTTGTGTTAAGATCCTCAAGAACCTCAACCACACCAATTATGATCATATGGTCCTAAATTCAACCCATCTTGCCCATGAATCTGAAGCGGTGGACCCAAAGGCGGGGCACTTCTTTGTGCGGTTTGCTAGCCTCTGTGCTACAGCTAAGCCCAATCCCAATTTCAATTTCATCCTCCAAAATGACGGACGAGTCTACCCGTTTTCCAAGGTCGCCACAAACGGCGTCGCCGCTGCCAACACTCTCGCCGTACCGCCGTTGTCGCCATTAGCCCGAGTTCACCGTCACACGCTGCACTGCAATGGGAGTTCGTGGGTCGAGCCCAAGTCGAGTGCCGCCGATGGGTTATCCTGGTGGTCCGGTTCGGATGATGAAGAAGACTTGCGAGAAGAAGAAATGGTCGGAGGTAATATTAGTTTCAAGAAGGCTTCGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.07

Weight (kDa)

5.89

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 303
AciI CCGC 7 cut(s) 73, 197, 212, 229, 339, 362, 444
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 1 cut(s) 166
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 3 cut(s) 211, 212, 450
AgsI TTSAA 4 cut(s) 94, 171, 274, 529
AhlI ACTAGT 1 cut(s) 83
AjnI CCWGG 1 cut(s) 458
AjuI GAANNNNNNNTTGG 4 cut(s) 254, 260, 286, 292
AluBI AGCT 1 cut(s) 254
AluI AGCT 1 cut(s) 254
AlwI GGATC 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 341, 400
ApoI RAATTY 1 cut(s) 166
AspS9I GGNCC 3 cut(s) 160, 202, 465
AsuHPI GGTGA 3 cut(s) 37, 65, 380
AsuNHI GCTAGC 1 cut(s) 236
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 3 cut(s) 160, 202, 465
BaeGI GKGCMC 1 cut(s) 219
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 328, 387
BccI CCATC 3 cut(s) 2, 183, 443
BceAI ACGGC 3 cut(s) 341, 346, 349
BciT130I CCWGG 1 cut(s) 460
BclI TGATCA 1 cut(s) 151
BcuI ACTAGT 1 cut(s) 83
BfaI CTAG 3 cut(s) 84, 237, 544
BfmI CTRYAG 1 cut(s) 249
BisI GCNGC 4 cut(s) 339, 342, 401, 444
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 4 cut(s) 340, 343, 402, 445
Bme1390I CCNGG 1 cut(s) 460
Bme18I GGWCC 3 cut(s) 160, 202, 465
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 3 cut(s) 160, 202, 465
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 460
BmsI GCATC 1 cut(s) 46
BmtI GCTAGC 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 467
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 212, 450
Bse1I ACTGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 3 cut(s) 13, 279, 481
BseLI CCNNNNNNNGG 3 cut(s) 211, 212, 450
BseMI GCAATG 1 cut(s) 415
BseNI ACTGG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 2 cut(s) 328, 387
BsgI GTGCAG 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 3 cut(s) 211, 212, 450
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 219, 432
Bsp143I GATC 2 cut(s) 120, 151
Bsp1720I GCTNAGC 1 cut(s) 255
BspACI CCGC 7 cut(s) 73, 197, 212, 229, 339, 362, 444
BspLI GGNNCC 1 cut(s) 204
BspOI GCTAGC 1 cut(s) 240
BspPI GGATC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 2 cut(s) 120, 151
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 1 cut(s) 392
BstACI GRCGYC 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 14, 238
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 3 cut(s) 13, 279, 481
BstKTI GATC 2 cut(s) 123, 154
BstMBI GATC 2 cut(s) 120, 151
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 328, 387
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BtsCI GGATG 3 cut(s) 13, 279, 481
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 14, 238
Cfr13I GGNCC 3 cut(s) 160, 202, 465
CseI GACGC 1 cut(s) 322
Csp6I GTAC 1 cut(s) 359
CviAII CATG 4 cut(s) 13, 44, 60, 185
CviJI RGCY 6 cut(s) 240, 254, 259, 380, 430, 536
CviKI_1 RGCY 6 cut(s) 240, 254, 259, 380, 430, 536
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 1 cut(s) 255
DpnI GATC 2 cut(s) 122, 153
DpnII GATC 2 cut(s) 120, 151
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 432
Eco47I GGWCC 3 cut(s) 160, 202, 465
Eco57I CTGAAG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 458
EcoT14I CCWWGG 1 cut(s) 315
EcoT22I ATGCAT 2 cut(s) 18, 61
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 4 cut(s) 16, 47, 63, 188
FaiI YATR 8 cut(s) 14, 18, 45, 61, 150, 156, 158, 186
FatI CATG 4 cut(s) 12, 43, 59, 184
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 156
FbaI TGATCA 1 cut(s) 151
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 4 cut(s) 339, 342, 401, 444
FokI GGATG 3 cut(s) 20, 266, 488
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 4 cut(s) 339, 342, 401, 444
FspBI CTAG 3 cut(s) 84, 237, 544
GluI GCNGC 4 cut(s) 339, 342, 401, 444
HapII CCGG 1 cut(s) 468
HgaI GACGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 4 cut(s) 16, 47, 63, 188
HinfI GANTC 2 cut(s) 188, 300
HpaII CCGG 1 cut(s) 468
HphI GGTGA 3 cut(s) 37, 65, 380
Hpy166II GTNNAC 3 cut(s) 202, 304, 388
Hpy188I TCNGA 4 cut(s) 193, 475, 512, 541
Hpy188III TCNNGA 2 cut(s) 127, 529
Hpy8I GTNNAC 3 cut(s) 202, 304, 388
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 4 cut(s) 31, 44, 113, 526
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4V TGCA 4 cut(s) 16, 59, 403, 408
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpyF3I CTNAG 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 4 cut(s) 16, 47, 63, 188
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 120, 151
LpnPI CCDG 4 cut(s) 111, 445, 472, 481
Lsp1109I GCAGC 2 cut(s) 328, 387
LweI GCATC 1 cut(s) 46
MaeI CTAG 3 cut(s) 84, 237, 544
MaeIII GTNAC 1 cut(s) 392
MalI GATC 2 cut(s) 122, 153
MboI GATC 2 cut(s) 120, 151
MboII GAAGA 5 cut(s) 59, 494, 497, 509, 512
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 2 cut(s) 219, 432
MluCI AATT 4 cut(s) 145, 166, 268, 274
MlyI GAGTC 1 cut(s) 309
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 7 cut(s) 16, 80, 134, 143, 251, 293, 506
Mph1103I ATGCAT 2 cut(s) 18, 61
MseI TTAA 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 1 cut(s) 460
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 1 cut(s) 370
NdeI CATATG 1 cut(s) 156
NdeII GATC 2 cut(s) 120, 151
NheI GCTAGC 1 cut(s) 236
NlaIII CATG 4 cut(s) 16, 47, 63, 188
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 392
NsiI ATGCAT 2 cut(s) 18, 61
NspI RCATGY 1 cut(s) 16
PaeI GCATGC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 4 cut(s) 340, 343, 402, 445
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 458
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 3 cut(s) 160, 202, 465
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 4 cut(s) 339, 342, 401, 444
Sau3AI GATC 2 cut(s) 120, 151
Sau96I GGNCC 3 cut(s) 160, 202, 465
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 2 cut(s) 219, 432
SetI ASST 7 cut(s) 27, 55, 72, 135, 256, 321, 517
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 3 cut(s) 160, 202, 465
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 83
SphI GCATGC 1 cut(s) 16
Sse9I AATT 4 cut(s) 145, 166, 268, 274
SsiI CCGC 7 cut(s) 73, 197, 212, 229, 339, 362, 444
SspI AATATT 1 cut(s) 520
SspMI CTAG 3 cut(s) 84, 237, 544
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 392
TaqI TCGA 2 cut(s) 426, 437
TasI AATT 4 cut(s) 145, 166, 268, 274
TauI GCSGC 2 cut(s) 341, 446
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 410
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 2 cut(s) 341, 400
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 4 cut(s) 60, 201, 268, 495
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 1 cut(s) 410
VpaK11BI GGWCC 3 cut(s) 160, 202, 465
XapI RAATTY 1 cut(s) 166
XceI RCATGY 1 cut(s) 16
XmiI GTMKAC 1 cut(s) 303
XspI CTAG 3 cut(s) 84, 237, 544
Zsp2I ATGCAT 2 cut(s) 18, 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.